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1.
Small ; : e2104186, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34622576

RESUMO

NiS1.23 Se0.77 nanosheets closely attached to the internal surface of hollow mesoporous carbon sphere (HMCS) to form a NiS1.23 Se0.77 nanosheets embedded in HMCS (NSSNs@HMCS) composite as the anode of sodium ion batteries (SIBs) is reported by a facile synthesis route. The anode exhibits a superior reversible capacity (520 mAh g-1 at 0.1 A g-1 ), impressive coulombic efficiency (CE) of up to 95.3%, a high rate capacity (353 mAh g-1 at 5.0 A g-1 ), excellent capacity retention at high current density (95.6%), and high initial coulombic efficiency (ICE) (95.1%). Firstly, the highest ICE for NiS2 /NiSe2 -based anode can be ascribed to ultrathin layered structure of NiS1.23 Se0.77 nanosheet and highly efficient electron transfer between the active material and HMCS. Secondly, the optimized NiS2 /NiSe2 heterostructure at the nanoscale of the inside HMCS is formed after the first discharge/charge cycles, which can provide rich heterojunction interfaces/boundaries of sulfide/selenides to offer faster Na+ pathways, decrease the Na+ diffusion barriers, increase electronic conductivity, and limit the dissolution of polysulfides or polyselenides in the electrolyte. Finally, the hollow structure of the HMCS accommodates the volume expansion, prevents the pulverization and aggregation issues of composite materials, which can also promote outstanding electrochemical performance.

2.
Langmuir ; 37(35): 10461-10468, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34431681

RESUMO

A colloidal nanocrystal cluster (CNC) is a hierarchical nanostructure formed by clustering several nanocrystals into one nano-ensemble, which may exhibit unique optical or catalytic properties different from individual nanocrystals owing to the mutual interactions among neighboring component nanocrystals. However, there is still no universal synthetic route that could be applicable to diverse material compositions with precisely controlled hierarchical structures (i.e., nanocrystal number density, component nanocrystal size, and overall diameter of the CNC) up to now. Herein, a general and novel synthetic strategy was reported for crafting a wide range of inorganic CNCs (i.e., noble metal, semiconductor, and metal oxide) via utilizing amphiphilic star-like poly(4-vinylpyridine)-block-polystyrene diblock copolymers as nanoreactors prepared by sequential atom transfer radical polymerization. The hierarchical structure of rationally designed CNCs could be readily tailored by varying the P4VP molecular weight of star-like nanoreactors and the parameter optimization during the CNC preparation process, which was inaccessible by conventional synthetic methods.

3.
ACS Appl Mater Interfaces ; 13(33): 39806-39818, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34387459

RESUMO

Silver nanomaterials have attracted a great deal of interest due to their broad-spectrum antimicrobial activity. However, it is still challenging to balance the high antibacterial efficiency with low damage to biological cells of silver nanostructures, especially when the diameter decreases to less than 10 nm. Here, we developed a new type of Ag nanohybrid material via a unimolecular micelle template method, which presents amazing antibacterial activities and almost noncytotoxicity. First, water-soluble multiarm star-shaped brushlike copolymer α-CD-g-[(PEO40-g-PAA50)-b-PEO5]18 was precisely synthesized and its micelle behavior in different solvents was revealed. Then, nanocrystal clusters assembled by Ag grains (Ag@Template NCs) were prepared through an in situ redox route using the unimolecular micelle of α-CD-g-[(PEO40-g-PAA50)-b-PEO5]18 as the soft template, AgNO3 as a precursor, and tetrabutylammonium borohydride (TBAB) as the reducing agent. The overall size of the achieved Ag@Template NCs is controlled by the template structure at around 40 nm (Dh in DMF), and the size of the Ag grain can be easily regulated from ∼1 to ∼5 nm by adjusting the feeding ratio of AgNO3/acrylic acid (AA) units in the template from 1:10 to 1:1. Benefitting from the structural design of the template, all Ag@Template NCs prepared here exhibit excellent dispersibility and chemical stability in different aqueous environments (neutral, pH = 5.5, and 0.9% NaCl physiological saline solution), which play a crucial role in the long-term storage and potential application in a complex physiological environment. The antibacterial and cytotoxicity tests indicate that Ag@Template NCs display much better performance than Ag nanoparticles (Ag NPs), which have a comparable overall size of ∼25 nm. The inhibitory capability of Ag@Template NCs to bacteria strongly depends on the grain size. Specifically, the Ag@Template-1 NC assembled by the smallest grains (1.6 ± 0.3 nm) presents the best antibacterial activity. For E. coli (-), the MIC value is as low as 5 µg/mL (0.36 µg/mL of Ag), while for S. aureus (+), the value is around 10 µg/mL (0.72 µg/mL of Ag). The survival rate of L02 cells and lactate dehydrogenase assay together illustrate the low cytotoxicity possessed by the prepared Ag@Template NCs. Therefore, the proposed Ag@Template NC structure successfully resolves the high reactivity, instability, and fast oxidation issues of the ultrasmall Ag nanoparticles, and integrates high antibacterial efficiency and nontoxicity to biological cells into one platform, which implies its broad potential application in biomedicine.


Assuntos
Antibacterianos/efeitos adversos , Antibacterianos/química , Nanopartículas Metálicas/química , Prata/química , Antibacterianos/metabolismo , Boroidretos/química , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Polímeros/química , Compostos de Amônio Quaternário/química , Staphylococcus aureus/efeitos dos fármacos , Propriedades de Superfície , Nanomedicina Teranóstica
4.
Front Oncol ; 11: 727698, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34422671

RESUMO

Background: Long non-coding RNAs (lncRNAs) display regulatory function flexibly in tumor onset and developments. Our study aimed to delve into the roles of lncRNA LINC01569 (LINC01569) in colorectal cancer (CRC) progression to study the potential mechanisms. Methods: The genetic expression profiles of miR-381-3p and LINC01569 were measured by RT-PCR. The subcellular localization of LINC01569 in CRC cells was identified using subcellular fractionation location. Loss-of-function assays were performed to explore the potential effects of LINC01569 on CRC progression. Dual-luciferase reporter analysis was employed to verify the binding connections among LINC01569, miR-381-3p, and RAP2A. Results: LINC01569 expression was distinctly increased in CRC. Curiously, if LINC01569 is removed, CRC cells will not migrate, proliferate, and invade remarkably. Molecular mechanism exploration uncovered that LINC01569 acted as a ceRNA competing with RAP2A to bind with miR-381-3p. Furthermore, rescue experiments corroborated the fact that miR-381-3p suppression reversed the inhibitory actions of LINC01569 knockdown on the expression of RAP2A and CRC progression. Conclusion: Overall, our findings indicate that LINC01569 plays a key role in CRC development by means of aiming at the miR-381-3p/RAP2A axis and can be equivalent to an underlying medicinal target to save CRC patients.

5.
Dalton Trans ; 50(33): 11458-11465, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34346462

RESUMO

Cobalt selenides based on the conversion reaction have been widely applied in lithium-ion batteries (LIBs) due to their high conductivity and high specific capacity. However, effectively suppressing the fast capacity fade caused by the irreversible Se/Co dissolution and serious volume change during the cycling process is still a challenge. Herein, a facile and efficient self-generated sacrificial template method is used to prepare Co0.85Se nanoparticles encapsulated in the inner wall of N-doped carbon matrix nanotubes (Co0.85Se@NCMT). In this strategy, the formation of stable Co-N/C and Se-C as well as enhancing the mechanical strength between active materials and N-doped carbon matrix nanotubes can critically affect the performance through suppressing the dissolution of Se/Co, decreasing energy band, promoting the shuttling of the ions/e- moving and mitigating the volume expansion during the charge-discharge process, which play a key role in improving the structure stability and electrochemical performance. Besides, Co0.85Se nanoparticles encapsulated in the robust carbon matrix inner wall can ensure good electron transfer and prevent the aggregation of nanoparticles, leading to superior electrochemical reversibility. Finally, carbon matrix nanotubes can provide sufficient space to effectively accommodate the volume changes of encapsulated Co0.85Se nanoparticles, thereby improving the cyclic stability. Based on the above advantages, as expected, the electrochemical investigations exhibited that the Co0.85Se@NCMT anode performs a stable reversible capacity of 462.9 mA h g-1 at a large current density of 5 A g-1 and a remarkable capacity retention of 99.5% after 800 cycles, suggesting its promising potential for the anode of LIBs.

6.
Phytother Res ; 35(9): 5214-5226, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34213784

RESUMO

Hawthorn (Crataegus pinnatifida Bunge. var. major) is an edible and medicinal fruit that is very common in food and traditional Chinese medicine. Corosolic acid (CA), a pentacyclic triterpenoid, which is an active component of hawthorn (Crataegus pinnatifida Bunge. var. major), has been exhibiting various pharmacological activities such as antidiabetic, antibacterial, anticancer, antiinflammatory, and antioxidant effects. The study aimed to evaluate the effect of CA on non-alcoholic steatohepatitis (NASH) in mice induced by 60 kcal% high-fat diet (HFD) and carbon tetrachloride (CCl4 ). CA lowered liver index and serum AST, ALT, TG, and TC levels compared to those in the model group. Histological analyses of the liver tissues of mice treated with CA revealed significantly decreased number of lipid droplets and alleviated inflammation and fibrosis. CA inhibited the transcripts of pro-fibrogenic markers (including α-SMA, collagen I, and TIMP-1) and the levels of pro-inflammatory cytokines (including TNF-α, IL-1ß, caspase-1, and IL-6) associated with hepatic fibrosis, and NF-κB translocation and TGF-ß1/Smad2 and AMPK pathways. In addition, CA reduced lipid accumulation via the regulation of AMPK and NF-κB activation in FFA-induced steatotic HepG2 cells. CA also decreased α-SMA, collagen I expressions, and Smad2 phosphorylation, which were reduced by TGF-ß1 treatment in LX2 cells. Our results suggested that CA ameliorated NASH through regulating TGF-ß1/Smad2, NF-κB, and AMPK signaling pathways, and CA could be developed as a potential health functional food or therapeutic agent for NASH patients.


Assuntos
Hepatopatia Gordurosa não Alcoólica , Transdução de Sinais/efeitos dos fármacos , Triterpenos , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Tetracloreto de Carbono , Dieta Hiperlipídica/efeitos adversos , Fígado/metabolismo , Cirrose Hepática , Camundongos , NF-kappa B/metabolismo , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Proteína Smad2 , Fator de Crescimento Transformador beta1/metabolismo , Triterpenos/farmacologia
7.
Genes Genomics ; 43(9): 1023-1033, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34129195

RESUMO

BACKGROUND: Retinoblastoma (RB) is the most prevalent primary intraocular malignancy, which commonly occurs during infant and childhood. OBJECTIVE: Our study aimed to investigate whether microRNA-9 (miR-9) could regulate RB cells and its mechanism. METHODS: qRT-PCR analysis was used to detect the expression of miR-9. In addition, to detect the migration of RB cells, wound healing assay was conducted. Xenograft tumor models in nude mice were also established, in order to assess the effects of miR-9 on tumor growth. qRT-PCR, luciferase reporter assay and western blot analysis were used to detect the target of miR-9. RESULTS: Initially, the expression level of miR-9 was significantly decreased in the RB tissues and blood samples from patients with RB. qRT-PCR, luciferase reporter assay and western blot analysis were used to confirm that PTEN was the target genes of miR-9 and it was negatively regulated by miR-9. When the expression of miR-9 was up-regulated, the cell viability, proliferation, migration and tumor formation were significantly suppressed. Furthermore, the expression level of PTEN was decreased after transfection of miR-9 mimic. Taken together, these results indicated that miR-9 might suppress the cell viability, proliferation, migration and tumor formation in RB by inhibiting PTEN. CONCLUSION: The in vitro and in vivo experiments demonstrated that miR-9 acts as a tumor suppressor function in RB cells and might serve as novel therapeutic targets for the treatment of RB.

8.
J Colloid Interface Sci ; 600: 421-429, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34023703

RESUMO

Multi-stimuli responsive fluorescence probe could pave the way for monitoring more complex environmental changes. Here we prepared multifunctional nanoparticle Fe3O4@SiO2@P(DMAEMA-co-TPEE), which displayed yolk-shell morphology with well-defined polymer brush. With superparamagnetic Fe3O4 component and pH/temperature dual sensitive PDMAEMA polymer brush, the as prepared nanoparticles (YS-NPs) exhibited as multi-stimuli responsive fluorescence probe for real-time visual monitoring of environmental changes such as magnetic field, temperature and pH. Such YS-NPs could also be applied as a sensitive detector for CO2 in aqueous solution. Notably, the solution of YS-NPs showed high colloidal stability during the environmental changes, and surface aggregation-induced emission (S-AIE) was proposed for the aggregation of TPE residue on the surface of YS-NPs.


Assuntos
Nanocompostos , Nanopartículas , Polímeros , Temperatura
9.
J Phys Chem Lett ; 12(13): 3456-3463, 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33792312

RESUMO

The past few years witnessed the rapid development of bottom-up synthesis strategies for preparing various nanostructures (i.e., nanoparticles, nanorods, nanowires, etc.) with distinct morphology-dependent properties. In this study, we reported a facile and efficient synthesis method for preparing anatase titanium dioxide (TiO2) nanorings based on multiarm, starlike amphiphilic polystyrene-b-poly(acrylic acid) (PS-b-PAA) diblock copolymers as nanoreactors which were prepared via a sequential atom-transfer radical polymerization (ATRP) technique followed by the conversion of polystyrene-b-poly(tert-butyl acrylate) (PS-b-PtBA) to PS-b-PAA. The outer PAA block of nanoreactors possessed carboxylic acid groups which could coordinate with a titanium precursor followed by high-temperature calcination to form crystalline TiO2 nanorings. The living nature of ATRP enabled the precise preparation of starlike diblock copolymer nanoreactors with a controlled length of each block (i.e., PtBA and PS), thereby tailoring the inner diameter and wall thickness of the resulting TiO2 nanorings, which were inaccessible to conventional routes.

10.
Bioinformatics ; 2021 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-33830205

RESUMO

SUMMARY: JavaScript-based Circos libraries have been widely implemented to generate interactive Circos plots in web applications. However, these libraries require either local installation, which requires the compilation of extra libraries, or extra data processing procedures to prepare input and configuration for each track of plot, which limits the utility and capability of integration with powerful R packages. In this report, we present interacCircos, an R package for creating interactive Circos plots through the integration of JavaScript-based libraries. interacCircos can simply and flexibly implement 14 track-plot functions and 7 auxiliary functions for presenting large-scale genomic data in interactive Circos plots. AVAILABILITY AND IMPLEMENTATION: InteracCircos and its manual are freely available at https://github.com/mrcuizhe/interacCircos. The online documentation is available at https://mrcuizhe.github.io/interacCircos_documentation/index.html under the GPL license. We thank the teams of BioCircos.js, BioCircos.R, NGCircos and circosJS for sharing the code.

11.
Front Genet ; 12: 658352, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33889181

RESUMO

Single-cell Assay Transposase Accessible Chromatin sequencing (scATAC-seq) has been widely used in profiling genome-wide chromatin accessibility in thousands of individual cells. However, compared with single-cell RNA-seq, the peaks of scATAC-seq are much sparser due to the lower copy numbers (diploid in humans) and the inherent missing signals, which makes it more challenging to classify cell type based on specific expressed gene or other canonical markers. Here, we present svmATAC, a support vector machine (SVM)-based method for accurately identifying cell types in scATAC-seq datasets by enhancing peak signal strength and imputing signals through patterns of co-accessibility. We applied svmATAC to several scATAC-seq data from human immune cells, human hematopoietic system cells, and peripheral blood mononuclear cells. The benchmark results showed that svmATAC is free of literature-based markers and robust across datasets in different libraries and platforms. The source code of svmATAC is available at https://github.com/mrcuizhe/svmATAC under the MIT license.

12.
Chem Commun (Camb) ; 57(19): 2400-2403, 2021 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-33543182

RESUMO

CoMn phosphide encapsulated in a nitrogen-doped graphene core-shell structure was successfully prepared with Prussian Blue complexes as the precursor and P atom doping at a high temperature. The core-shell heterostructure demonstrates an impressive catalytic performance of hydrogen evolution reaction over a broad pH range, i.e., the overpotentials reach 159, 190 and 279 mV at a current density of 20 mA cm-2 in the conditions of pH = 0, 14 and 7, and maintains excellent stability.

13.
Exp Ther Med ; 21(3): 255, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33603862

RESUMO

Retinoblastoma (RB) is one of the most common forms of childhood intraocular cancer. While the occurrence of RB is traditionally associated with dysregulation of the RB1 gene, efforts have been made to assess the role of several other pathways that may result in RB. The Notch signaling pathway has been identified as one of the sentinel pathways in retinal development and has been indicated to serve as a tumor suppressor. However, epigenetic modifications of the Notch signaling pathway, and their consequences on tumor establishment and progression, have received little attention. The present study attempted to elucidate the microRNA (miR)-mediated dysregulation of the Notch signaling pathway and its implications on tumor initiation. Upon recruitment of patients with RB (age, 4-25 months), the levels of miR-34b-5p were determined in tumor and adjacent healthy tissues. Simultaneously, the serum levels of miR-34b-5p were measured in tumor and healthy samples using reverse transcriptase-quantitative PCR (RT-qPCR). Binding of miR-34b-5p to Notch1 and Notch2 were confirmed bioinformatically. In vitro studies were performed in Y79 and Weri-Rb-1 RB cell lines. The cell lines were transfected with miR-34b-5p constructs and miR-34b-5p overexpression was confirmed using RT-qPCR. The impact of miR-34b-5p overexpression on cell growth and cancer stemness markers (Sox-2, Nanog, and CD133) was examined. The expression levels of Notch1 and Notch2 were evaluated in the presence of miR-34b-5p. The rescue of cell growth and cancer stemness phenotypes was evaluated by co-transfection of miR-34b-5p with Notch1 or Notch2. The results of the present study indicated that the expression levels of miR-34b-5p were reduced in patient tissues and serum samples compared with those in healthy tissues and samples. Notch1 and Notch2 expression level was negatively correlated with the expression level of miR-34b-5p. Overexpression of miR-34b-5p resulted in reduced cell proliferation, migration, invasion and cancer stemness compared with the control group. Further in vivo experiments confirmed the inhibitory effects of miR-34b-5p on RB cell proliferation. Upon co-transfection of miR-34b-5p with Notch1 or Notch2, these phenotypes were rescued with reversal of cell growth and tumor sphere formation. Collectively, the results indicated that miR-34b-5p functions as a tumor suppressor in RB via regulating the Notch signaling pathway. Therefore, miR-34b-5p may be explored for its utility as a therapeutic target in RB.

14.
Dig Dis Sci ; 66(5): 1658-1668, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-32524415

RESUMO

BACKGROUND AND AIMS: Information concerning deep radiological healing of perianal fistulas in Chinese patients with CD is limited. The present study aimed to establish the effectiveness of infliximab on CD-related perianal fistulas using magnetic resonance imaging (MRI) and identify predictors of deep radiological remission of fistulas. METHODS: We retrospectively reviewed patients with CD with draining perianal fistulas treated with infliximab and included only those who underwent clinical assessment and MRI before and after infliximab therapy. RESULTS: Among 178 patients who underwent repeated MRI and clinical assessment, 65.2% had complex fistulas. Post-infliximab therapy, 55.1% of patients with perianal fistulizing CD showed clinical remission and 26.4% presented a clinical response; 38.2% had deep radiological remission, and 34.3% had a partial response based on the Ng score; the Van Assche scores decreased obviously compared with baseline. Prolonged infliximab infusion (18 times) presented higher radiological remission rates in patients with CD with complex fistulas. Concomitant treatment with azathioprine increased the fistula healing rate compared with infliximab alone (50% vs. 36.9%, P < 0.001). Younger age at diagnosis of CD, proctitis and requiring perianal surgery were identified as predictors of poor deep radiological remission of fistulas. Eight of ten patients who stopped infliximab and switched to an alternative agent retained a status of fistula healing in the first year of follow-up. CONCLUSIONS: Infliximab induced deep radiological remission of perianal fistulas in Chinese patients with CD. Routine MRI should be used to monitor fistula healing. Patients with younger age at diagnosis of CD, proctitis, and/or requiring perianal surgery should receive combined therapy and careful monitoring.

15.
BMC Gastroenterol ; 20(1): 421, 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33308166

RESUMO

BACKGROUND: Clinicians aim to prevent progression of Crohn's disease (CD); however, many patients require surgical resection because of cumulative bowel damage. The aim of this study was to evaluate the impact of early intervention on bowel damage in patients with CD using the Lémann Index and to identify bowel resection predictors. METHODS: We analyzed consecutive patients with CD retrospectively. The Lémann Index was determined at the point of inclusion and at follow-up termination. The Paris definition was used to subdivide patients into early and late CD groups. RESULTS: We included 154 patients, comprising 70 with early CD and 84 with late CD. After follow-up for 17.0 months, more patients experienced a decrease in the Lémann Index (61.4% vs. 42.9%), and fewer patients showed an increase in the Lémann Index (20% vs. 35.7%) in the early compared with the late CD group. Infliximab and other therapies reversed bowel damage to a greater extent in early CD patients than in late CD patients. Twenty-two patients underwent intestinal surgery, involving 5 patients in the early CD group and 17 patients in the late CD group. Three independent predictors of bowel resection were identified: baseline Lémann index ≥ 8.99, disease behavior B1, and history of intestinal surgery. CONCLUSIONS: Early intervention within 18 months after CD diagnosis could reverse bowel damage and decrease short-term intestinal resection. Patients with CD with a history of intestinal surgery, and/or a Lémann index > 8.99 should be treated aggressively and monitored carefully to prevent progressive bowel damage.


Assuntos
Doença de Crohn , Doença de Crohn/tratamento farmacológico , Doença de Crohn/cirurgia , Humanos , Infliximab , Intestinos , Estudos Retrospectivos , Índice de Gravidade de Doença
16.
Medicine (Baltimore) ; 99(50): e23557, 2020 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-33327308

RESUMO

Intracerebral hemorrhage (ICH) is the second most common subtype of stroke with higher mortality and morbidity, and it lacks effective prognostic markers. The aim of this research is to construct newly valuable prognostic nomogram incorporating red blood cell distribution width (RDW) for ICH patients.We retrospectively analyzed 953 adult patients with ICH. The impacts of RDW on short-term mortality and functional prognosis were calculated using Akaike information criterion (AIC), Bayesian information criteria (BIC) and the area under the curve (AUC) respectively, which could be used to compare with Glasgow coma scale (GCS) and ICH score. The independent factors of prognosis were identified by univariate and multivariate logistic regression analysis. A nomogram based on RDW for nerve functional prognosis was further constructed and validated. Its clinical value was subsequently explored utilizing decision curve analysis.Cumulative clinical results were retrieved for 235 inpatients from Jan 2012 to June 2017. In 30-day mortality sets, GCS and ICH score had better prognostic performance than RDW (AUC: 0.929 and 0.917 vs 0.764; AIC: 124.101 and 134.188 vs 221.372; BIC: 131.021 and 141.107 vs 228.291). In 30-day functional prognosis sets, the consequences of evaluation systems were inconsistent. GCS was the best parameter for predicting outcome using AIC (262.350 vs 276.392 and 264.756) and BIC (269.269 vs 283.311 and 271.675). However, RDW was higher than GCS and ICH score considering AUC (0.784 vs 0.759 and 0.722). Age, GCS, RDW, platelet distribution width, and surgery were independent prognostic factors by multivariate logistic regression analysis, and those coefficients were used to formulate a nomogram. This nomogram can provide accurate prediction with the concordance index of 0.880 (95% CI, 0.837-0.922) higher than Harrell's concordance index of GCS system 0.759 (95% CI, 0.698-0.819) and RDW 0.784 (95% CI, 0.721-0.847). The calibration plots showed optimal consistency between bootstrap-predicted and the actual observed values of 30-day unfavorable prognosis. Decision curve analysis showed an increased net benefit for utilizing the nomogram.High RDW values are associated with an unfavorable outcome after ICH. The established nomogram incorporating RDW should be considered for a 30-day functional prognosis.


Assuntos
Hemorragia Cerebral/diagnóstico , Índices de Eritrócitos , Eritrócitos/patologia , Nomogramas , Idoso , Biomarcadores/sangue , Hemorragia Cerebral/mortalidade , Hemorragia Cerebral/patologia , Feminino , Escala de Coma de Glasgow , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Curva ROC , Estudos Retrospectivos , Análise de Sobrevida
17.
Adv Sci (Weinh) ; 7(18): 1903045, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32999824

RESUMO

SnS, is a promising anode material for lithium ion batteries (LIBs) and sodium ion batteries (SIBs), however, undergoes poor cyclic lifespan due to its huge volume changes and bad electroconductivity. Here, a modified CVD method is used to directly grow graphene-like carbon film on the surface of SnS nanosheet arrays which are supported by Co-, N-modified porous carbon fibers (CCF@SnS@G). In the strategy, the SnS nanosheet arrays confined into the integrated carbon matrix containing porous carbon fibers and graphene-like carbon film, perform a greatly improved electrochemical performance. In situ TEM experiments reveal that the vertical graphene-like carbon film can not only protect the SnS nanosheet from destruction well and enhance the conductivity, but also transforms SnS nanosheet into ultrafine nanoparticles to promote the electrochemical kinetics. Systematic electrochemical investigations exhibit that the CCF@SnS@G electrode delivers a stable reversible capacity of 529 mAh g-1 at a high current density of 5 A g-1 for LIBs and 541.4 mAh g-1 at 2 A g-1 for SIBs, suggesting its good potential for anode electrodes.

18.
ACS Appl Mater Interfaces ; 12(37): 42161-42168, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32840349

RESUMO

Silicon quantum dots (SiQDs) are explored for the first time as an efficient photocatalyst for visible-light-regulated reversible addition-fragmentation chain transfer (RAFT) polymerization. The fluorescence quenching confirmed the photoinduced electron transfer (PET) between SiQDs and RAFT reagents. Besides all features of controlled radical polymerization, the SiQDs catalyzed PET-RAFT polymerization also exhibit good temporal control, high chain-end fidelity, and versatility with diverse monomers. Moreover, amino-functionalized SiQDs can be easily coated on the surface of substrates (silicon wafer) owing to the electrostatic interaction, and play a dual role of polymer-substrate connector and photocatalyst for the surface-initiated PET-RAFT polymerization. The SiQD-coated wafer was also proved to be an efficient recycle photocatalyst for PET-RAFT polymerization.

19.
Genome Biol ; 21(1): 189, 2020 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-32746918

RESUMO

Long-read sequencing is promising for the comprehensive discovery of structural variations (SVs). However, it is still non-trivial to achieve high yields and performance simultaneously due to the complex SV signatures implied by noisy long reads. We propose cuteSV, a sensitive, fast, and scalable long-read-based SV detection approach. cuteSV uses tailored methods to collect the signatures of various types of SVs and employs a clustering-and-refinement method to implement sensitive SV detection. Benchmarks on simulated and real long-read sequencing datasets demonstrate that cuteSV has higher yields and scaling performance than state-of-the-art tools. cuteSV is available at https://github.com/tjiangHIT/cuteSV .

20.
Drug Des Devel Ther ; 14: 2959-2975, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801637

RESUMO

Background and Purpose: The traditional Chinese medicine, diosgenin (Dio), has attracted increasing attention because it possesses various therapeutic effects, including anti-tumor, anti-infective and anti-allergic properties. However, the commercial application of Dio is limited by its extremely low aqueous solubility and inferior bioavailability in vivo. Soluplus, a novel excipient, has great solubilization and capacity of crystallization inhibition. The purpose of this study was to prepare Soluplus-mediated Dio amorphous solid dispersions (ASDs) to improve its solubility, bioavailability and stability. Methods: The crystallization inhibition studies were firstly carried out to select excipients using a solvent shift method. According to solubility and dissolution results, the preparation methods and the ratios of drug to excipient were further optimized. The interaction between Dio and Soluplus was characterized by differential scanning calorimetry (DSC), fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), powder X-ray diffraction (PXRD) and molecular docking. The pharmacokinetic study was conducted to explore the potential of Dio ASDs for oral administration. Furthermore, the long-term stability of Dio ASDs was also investigated. Results: Soluplus was preliminarily selected from various excipients because of its potential to improve solubility and stability. The optimized ASDs significantly improved the aqueous solubility of Dio due to its amorphization and the molecular interactions between Dio and Soluplus, as evidenced by dissolution test in vitro, DSC, FT-IR spectroscopy, SEM, PXRD and molecular docking technique. Furthermore, pharmacokinetic studies in rats revealed that the bioavailability of Dio from ASDs was improved about 5 times. In addition, Dio ASDs were stable when stored at 40°C and 75% humidity for 6 months. Conclusion: These results indicated that Dio ASDs, with its high solubility, high bioavailability and high stability, would open a promising way in pharmaceutical applications.

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