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Molecules ; 26(2)2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-33435259


Epilobium angustifolium L. is applied as an antiseptic agent in the treatment of skin diseases. However, there is a lack of information on human skin penetration of active ingredients with antioxidative potential. It seems crucial because bacterial infections of skin and subcutaneous tissue are common and partly depend on oxidative stress. Therefore, we evaluated in vitro human skin penetration of fireweed ethanol-water extracts (FEEs) by determining antioxidant activity of these extracts before and after penetration study using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and Folin-Ciocalteu methods. Microbiological tests of extracts were done. The qualitative and quantitative evaluation was performed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC-UV) methods. The in vitro human skin penetration using the Franz diffusion chamber was assessed. The high antioxidant activity of FEEs was found. Gallic acid (GA), chlorogenic acid (ChA), 3,4-dihydroxybenzoic acid (3,4-DHB), 4-hydroxybenzoic acid (4-HB), and caffeic acid (CA) were identified in the extracts. The antibacterial activities were found against Serratia lutea, S. marcescens, Bacillus subtilis, B. pseudomycoides, and B. thuringiensis and next Enterococcus faecalis, E. faecium, Streptococcus pneumoniae, Pseudomonas aeruginosa, and P. fluorescens strains. In vitro penetration studies showed the penetration of some phenolic acids and their accumulation in the skin. Our results confirm the importance of skin penetration studies to guarantee the efficacy of formulations containing E. angustifolium extracts.

Anti-Infecciosos , Antioxidantes , Bactérias/crescimento & desenvolvimento , Bassia scoparia/química , Extratos Vegetais , Absorção Cutânea , Pele/metabolismo , Anti-Infecciosos/química , Anti-Infecciosos/farmacocinética , Anti-Infecciosos/farmacologia , Antioxidantes/química , Antioxidantes/farmacocinética , Antioxidantes/farmacologia , Etanol/química , Humanos , Extratos Vegetais/química , Extratos Vegetais/farmacocinética , Extratos Vegetais/farmacologia , Pele/microbiologia , Água/química
Sci Total Environ ; 709: 136112, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31884294


Microflora of marine waters and sediments play a significant role not only in the course of natural changes and circulation of elements in this environment, but can also be a decisive factor during erosion processes of various submerged objects or structures such as wrecks, sunken objects, pipelines, etc. The bacteria which have a significant influence on corrosion processes include aerobic and anaerobic sulfur bacteria as well as iron bacteria. Particular importance is attributed to sulfide reducing bacteria (SRB) which occur in waters, bottom sediments, creating biofilms on metal surface, and may start a process known as microbial induced corrosion (MIC). The occurrence of these bacteria and their populations indicates potential and intensification of corrosion processes. The aim of the study was to determine the number of microorganisms from various groups in the Baltic Sea sediments, especially those that are active in biological corrosion processes. This data can be used for a study comprehensive of corrosive processes in the marine environment, and as a result, to carry out a real assessment of the risks arising from the release of toxic substances into the environment from conventional and chemical weapons lying on the bottom caused by progressing corrosive processes, including biological corrosion. The article presents the results of research on the presence and accounts of microorganisms in bottom sediment samples taken from the dump sites of chemical and conventional ammunition in the Baltic Sea. Particular attention was paid to halophilic and halotolerant bacteria due to their significant contribution to both corrosion and decomposition processes of CWA and explosives, particularly in the saltwater environment. There were high and quite similar numbers of mesophilic, psychrophilic and halophilic bacteria in the studied samples, while significantly smaller numbers of anaerobic bacteria. The amount of mesophilic bacteria ranges from 6.42*103 to 2.85*107, on average 4.5*106 in 1 g DM, psychrophilic from 5.73*103 in1g DM to 4.11*107 in 1 g DM, on average 6.44*106 in 1 g DM, whereas for halophylic from 4.09*102 to 5.60*107, on average 5.83*106 in1g DM. The numbers of fungi were at a much lower level and ranged from 4.65*101 to 8.08*102, on average 2.63*102 in 1 g DM. mesophilic fungi and from 0 to 1.60*103, on average 3.58*102 in 1 g DM for psychrophilic fungi. The presence of sulfide reducing bacteria (SRB) was also detected in large populations, especially when they were isolated by means of the medium prepared with seawater (from 9.72 to 5.36*102, on average 2.52*102 in 1 g DM), which indicates their adaptation to the saline environment and promotes of biological anaerobic processes, including corrosive processes. No significant amounts of iron bacteria were found, probably due to the small amount of oxygen in the sampling area. The small number of iron bacteria indicates that primarily anaerobic sulfide reducing bacteria (SRB) have the major part in the microbial corrosion phenomena.

Archaea , Bactérias , Países Bálticos , Corrosão , Microbiota , Água do Mar
Open Life Sci ; 14: 537-548, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33817190


The objective of the present study was to compare the effect of rhamnolipids on the microbial biomass content and the activity of dehydrogenases (DHA), acid phosphatase (ACP), alkaline phosphatase (ALP), and urease (URE) in soil contaminated with two types of coal tar creosote: type C and type GX-Plus. The experiment was carried out on samples of sandy clay loam under laboratory conditions. Coal tar creosote was added to soil samples at a dose of 0 and 10 g·kg-1 DM, along with rhamnolipids at a dose of 0, 10, 100, and 1000 mg·kg-1 DM. The humidity of the samples was brought to 60% maximum water holding capacity, and the samples were incubated at 20°C. Microbial and biochemical parameters were determined on days 1, 7, 21, and 63. The obtained results demonstrated that the addition of rhamnolipids did not result in any significant changes in the activity of the determined parameters in the uncontaminated soil. However, it was observed that the application of these biosurfactants, particularly at the dose of 1000 mg·kg-1 DM, largely decreased the effect of coal tar creosote on the determined parameters. Moreover, the microbial biomass and the activity of ALP and URE were found to be the best indicator of bioremediation of soil contaminated with coal tar creosote.

Environ Sci Pollut Res Int ; 25(28): 28642-28653, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30094671


Tar oil is a complex mixture of hydrocarbon compounds obtained from high-temperature distillation of coal tar. It has been used for over 100 years from now to protect wood and has been applied to wood products, primary utility poles, and railroad ties by pressure methods. Composition of the tar oil depends on the source and typically consists of 85% polycyclic aromatic hydrocarbons (PAHs), 10% phenolic compounds, and 5% heterocyclic compounds. In this research, we performed the laboratory experiment to compare two types of tar oil: C and GX-Plus, and their effects on P-cycling enzymes (phosphatases) in sandy loam and loamy sand. Tar oil was applied to soil samples at the following doses: 2, 10, and 50 g kg-1. Soil without tar oil was used as a control sample. The experiment showed that the contamination of soil with tar oil affects the enzyme activities measured and with this most probably the P-cycle in soil. Phosphomonoesterases were the most sensitive to the contamination of soil with both type of tar oil: typeC and type GX-Plus. Greater changes in the enzymatic activity were observed in the loamy sand. Moreover, the type C tar oil demonstrated higher toxicity for phosphatases than type GX-Plus.

Alcatrão/química , Hidrocarbonetos/química , Monoéster Fosfórico Hidrolases/metabolismo , Poluentes do Solo/química , Solo/química , Alcatrão/análise , Hidrocarbonetos/análise , Monoéster Fosfórico Hidrolases/química , Polônia , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/química , Poluentes do Solo/análise
Antonie Van Leeuwenhoek ; 111(9): 1493-1507, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29460207


This article describes methods of treatment for avian zoonoses, modern antibiotic therapy and drug resistance of selected pathogens, which pose a threat to the population's health. A tabular form has been used to present the current data from the European Union from 2011 to 2017 regarding human morbidity and mortality and the costs incurred by national health systems for the treatment of zoonoses occurring in humans and animals. Moreover, the paper includes descriptions of selected diseases, which indirectly affect birds. Scientists can obtain information regarding the occurrence of particular diseases, their aetiology, epidemiology, incubation period and symptoms caused by dangerous microorganisms and parasites. This information should be of particular interest for people who have frequent contact with birds, such as ornithologists, as well as veterinarians, farm staff, owners of accompanying animals and zoological workers. This paper presents a review used for identification and genetic characterization of bacterial strains isolated from a variety of environmental sources, e.g., bird feathers along with their practical application. We describe the bacterial, viral and fungal serotypes present on avian feathers after the slaughter process. This review also enables us to effectively identify several of the early stages of infectious diseases from heterogeneous avian research material.

Doenças das Aves/microbiologia , Aves , Doenças Transmissíveis/microbiologia , Doenças Transmissíveis/transmissão , Plumas/microbiologia , Zoonoses/transmissão , Animais , Doenças das Aves/transmissão , Doenças Transmissíveis/epidemiologia , Reservatórios de Doenças/microbiologia , União Europeia/estatística & dados numéricos , Humanos , Zoonoses/epidemiologia , Zoonoses/microbiologia
Environ Sci Pollut Res Int ; 21(18): 10765-74, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24875308


This study was aimed at investigating the physicochemical and microbiological changes that took place during the ageing process of spent bleaching earth in the presence of autochthonous microorganisms. Research material included fresh spent bleaching earth (SBE0) and the same material after 3 years of storage at the constant temperature of 20 °C, without aeration and moistening (SBE3). Changes in the chemical composition of analysed waste material were observed during its ageing process point to a spontaneous bioconversion of fat substance towards formation and/or release of free saturated fatty acids C16:0 and C18:0 (14.3 g 100 g(-1) D.M.), triterpenes (8.48 g 100 g(-1) D.M.), cholesterol (3.29 g 100 g(-1) D.M.), small quantities of carbohydrates and esters (0.80 g 100 g(-1) D.M.). This process was accompanied by other changes in physicochemical parameters of the waste material, such as colour, odour and viscosity, decrease in fat content from 28.27 to 24.6 % and that of soluble forms of metals (Mo, Cu, Fe, Zn, Ni, Cr and Mn), ranging from 25 to 75 %, and an increase in pH, from 3.85 to 4.2. At the same time, changes in the microbial consortium were observed.

Ácidos Graxos/química , Consórcios Microbianos , Eliminação de Resíduos/métodos , Microbiologia do Solo , Poluentes do Solo/química , Triterpenos/química , Resíduos , Biodegradação Ambiental , Ácidos Graxos/análise , Ácidos Graxos Monoinsaturados , Concentração de Íons de Hidrogênio , Metais Pesados/análise , Óleos Vegetais/química , Polônia , Óleo de Brassica napus , Poluentes do Solo/análise , Temperatura , Fatores de Tempo , Triterpenos/análise