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1.
J Forensic Sci ; 65(1): 84-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31454431

RESUMO

Impressions of friction ridges left on a surface are important evidence for identifying an individual and can be used to confirm his or her presence at the scene of an incident. Factors influencing the durability of fingerprints include the physiological characteristics and cleanliness of the individual, environmental factors, and time. The aim of the study was to determine the effect of atmospheric factors, gender, hand cleanliness and time on the width of friction ridge impressions and the amount of material forming the print. The research shows that factors such as gender, ambient conditions, and hand cleanliness affect the width of the ridge impressions and the quantity of material forming the print. The passage of time significantly reduces both the width of the ridge impressions and the quantity of the material forming the print.


Assuntos
Dermatoglifia , Higiene das Mãos , Manejo de Espécimes , Adulto , Escuridão , Exposição Ambiental , Feminino , Humanos , Luz , Masculino , Microscopia , Fatores Sexuais , Creme para a Pele , Temperatura , Fatores de Tempo , Adulto Jovem
2.
Biol Trace Elem Res ; 193(2): 555-563, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31030384

RESUMO

The aim of the study was to compare the mineral content between the peel and the pulp of citrus fruits and to determine which citrus fruit, among orange (Citrus sinensis), pomelo (Citrus maxima), mandarin (Citrus reticulata Blanco), lemon (Citrus limon), key lime (Citrus aurantifolia), and red, yellow, or green grapefruit (Citrus paradisi), is the richest in minerals. The research material consisted of fresh citrus fruits belonging to the genus Citrus L in the family Rutaceae. The fruits were purchased at a supermarket at one time. To prepare laboratory samples, each fruit was cut in half, and one half was homogenized, treating the sample as a whole (peel + flesh), while the other half was peeled and the pulp (F) and peel (P) were homogenized separately. To determine the content of minerals (Na+, K+, Ca+2, Mg+2, Fe+2, Zn+2, Cu+2, Mn+2, and Se+2), the samples were mineralized and analyzed using an Analytik Jena PlasmaQuant PQ 9000 inductively coupled plasma optical emission spectrometer. The content of macro- and micronutrients in the peel of most of the fruits far exceeded their quantity in the pulp. Oranges and pomelos are the fruits richest in iron and copper, so they could be recommended in cases such as hemoglobin production disorders resulting from a deficiency of these elements. Oranges can additionally enrich the body with potassium, phosphorus, and manganese, while lime can be a source of calcium, zinc, sodium, and especially potassium. It should also be noted that all citrus fruits are a very valuable source of potassium, which is needed to ensure the water and electrolyte balance.

3.
Eur J Pharm Sci ; 143: 105169, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31785383

RESUMO

The article describes the preparation and characterization of 3D-printed tablets with bicalutamide obtained using two-material co-extrusion-based fused deposition modeling (FDM). This method is a modification of typical two-material FDM where separate nozzles are used to print from two filaments. In this work we used a ZMorph® 3D printer with DualPro printhead which allows us to co-extrude two filaments through a single nozzle. This approach gives the opportunity to modify tablet properties in a wide range, especially the dissolution rate, by producing dosage forms with a complex design. The great advantage of this method is that switching between immediate dosage form and controlled release does not require any change in the 3D-printer set-up. We checked the accuracy of co-extrusion printing simply by weighing the amounts of soluble and insoluble material in the printed object as well as calculating the volumes of the printed objects from micro computed tomography (µ-CT) images. We printed several tablets with a different design including simple one-material tablets, two- and three-compartment tablets with various internal structure and composition of the printing path. The dissolution tests were conducted in sink and non-sink conditions. We obtained tablets with desired bicalutamide dissolution profiles, i.e. immediate, controlled, and combined. The formation of spatial matrix slows down the dissolution in controlled and combined release bicalutamide tablets what was confirmed by µ-CT analysis before and after dissolution.

4.
Cent European J Urol ; 72(3): 252-257, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31720026

RESUMO

Introduction: The aim of this single centre retrospective study was to analyse the results of second resection (repeat transurethral resection of bladder tumour - reTURBT) after a macroscopically complete resection of T1 urothelial bladder tumour and to identify prognostic factors for absence of residual disease (T0) in the second resection of T1 bladder cancer. Material and methods: Patients with T1 bladder cancer diagnosed in a macroscopically complete initial resection who underwent second resection within 12 weeks were included into the retrospective analysis. Based on the presence or absence of residual disease, patients were grouped for further analysis. Univariate and multivariable logistic regressions were performed to identify potential prognostic factors. Results: Among the 139 patients who met the inclusion criteria, 96 (69.1%) had no residual disease (T0) and 43 (30.9%) had residual disease in the second resection (including muscle invasive bladder cancer in 2.2%). Adjusted odds ratios (OR) of T0 status obtained from the final model were as follows: detrusor muscle presence in the first resection (OR 3.05; 95% CI 1.12-8.35, p = 0.03), immediate post-operative intravesical mitomycin C administration after the first TURBT (OR 2.52, 95% CI 1.12-5.68; p = 0.03) and primary bladder cancer setting (OR 2.45, 95% CI 1.10-5.47; p = 0.03). Conclusions: Our results add evidence regarding the importance of detrusor muscle presence in the first TURBT. Identification of predictors of T0 status at second resection could help design prospective studies assessing the possibility to avoid re-resection in selected patients with T1 bladder cancer without compromising oncological outcomes.

5.
Clin Genitourin Cancer ; 17(6): e1203-e1211, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31588010

RESUMO

INTRODUCTION: Tumor regression grades (TRGs) quantify the pathologic response to neoadjuvant chemotherapy (NAC). The aim of the study was to investigate the prognostic value of TRGs in combination with the TNM classification in an independent cohort of patients with muscle-invasive bladder cancer (MIBC) treated with NAC followed by radical cystectomy (RC) in a retrospective setting. PATIENTS AND METHODS: Patients treated with a complete course of NAC followed by RC for MIBC between December 2012 and December 2017 were enrolled in the study. TRGs were determined in RC specimens. Data were collected preoperatively, and the follow-up was continued up to August 2018. Kaplan-Meier curves and the Cox proportional hazards model were used to compare survival probabilities between major responders (no MIBC, < ypT2 and ypN0), partial responders (≥ ypT2 or ypN+ and TRG2), and non-responders (≥ ypT2 or ypN+ and TRG3). RESULTS: A group of 70 patients with a median age of 64 years (interquartile range, 58-67 years) was analyzed. There were 36 major responders, 21 partial responders, and 13 non-responders. In comparison with a major response, a partial response was associated with a hazard ratio of 9.44 (95% confidence interval, 1.10-80.89; P = .04) and non- responders showed a hazard ratio of 17.85 (95% confidence interval, 2.18-145.85; P = .007) for death. CONCLUSIONS: The study confirms the prognostic value of the pathologic response to NAC. Determination of TRGs is straightforward, provides valuable information, and could be easily included in the standard pathologic examination of RC surgical specimen. Prospective studies are needed to establish the role of TRG in routine clinical practice.

6.
Cent European J Urol ; 72(2): 100-105, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31482015

RESUMO

Introduction: Even though the survival benefit of neoadjuvant chemotherapy (NAC) in the treatment of muscle invasive bladder cancer (MIBC) is well established, NAC has not been widely used in Poland until recently. The aim of our study was to evaluate the utilization of NAC and its association with survival in MIBC. Material and methods: Patients who underwent radical cystectomy (RC) for MIBC between December 2012 and December 2017 were included in the study. Data were collected in the perioperative period and long-term observation was continued up to August 2018. Kaplan-Meier curves were used to estimate the probability of survival. Results: A sample of 155 patients with a median age of 65 (IQR: 60-69) years was analyzed. In this group, 79 patients (51%) were treated with NAC prior to RC. Patients in the NAC+RC group were younger, more often had a positive smoking history, and had lower preoperative levels of hemoglobin, white blood cells and C-reactive protein. A 90-day complication rate and mortality were similar in both groups and in the entire cohort were equal to 64.5% and 5.2%, respectively. The overall survival (OS) was on average 150 days longer in the RC+NAC group compared to the RC-only group when patients were followed-up for 3 years (95%CI:3 4 - 267; p = 0.011). Conclusions: We demonstrated a high utilization of NAC at our institution. The use of NAC was associated with a better prognosis than RC alone and was not associated with an increased morbidity or mortality. Our results support the use NAC as a safe and effective treatment modality in MIBC.

7.
J Forensic Sci ; 64(3): 711-716, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30577070

RESUMO

The aim of the study was to determine the effect of gender, body mass index, and time on the width of friction ridge impressions in fingerprints and the content of sweat and sebum forming the print. The research showed that the friction ridge impressions of women are significantly wider than those of men, and this tendency persists throughout the existence of the print. The differences may result from the anatomical structure of the skin, as well as from the use of protective products such as creams, which are used more often by women. The width of friction ridge impression is similar in underweight and overweight people, but greater than in those with normal body weight, and the amount of sweat and sebum increases with body weight. The passage of time significantly reduces both the width of the friction ridge impression and the content of sweat and sebum secretions in the print.


Assuntos
Dermatoglifia , Adulto , Índice de Massa Corporal , Feminino , Humanos , Modelos Lineares , Masculino , Microscopia , Sebo/fisiologia , Fatores Sexuais , Suor/fisiologia , Fatores de Tempo , Adulto Jovem
8.
J Anim Physiol Anim Nutr (Berl) ; 102(6): 1720-1730, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30225850

RESUMO

The objective of the study was to compare the efficiency of dietary supplementation of two species of yeast, Yarrowia lipolytica (YL) and Saccharomyces cerevisiae (SC), with or without the addition of a probiotic, on growth performance, basic nutrients digestibility and blood profile in piglets. The experiment was conducted on 360 weaned piglets (180 barrows and 180 gilts). Each treatment comprised 60 piglets. The piglets (27-day-old) were assigned to the following dietary treatments: (a) basal control (C) diet, (b) C diet + probiotic (Bacillus licheniformis and Bacillus subtilis-2 × 109  CFU/kg of feed), (c) C diet + 3% Y. lipolytica (YL-2 × 1010  CFU/g), (d) C diet + 3%YL + probiotic, (e) C diet + 3% S. cerevisiae (SC-3 × 1010  CFU/g) and (f) C diet + 3% SC + probiotic. Blood for analysis was collected from a total of 36 piglets at the end of the experiment, that is, 56 days after weaning. During the entire experiment, significantly greater weight gain was observed in the piglets receiving YL. The addition of the probiotic supplement and SC yeast reduced the daily feed intake during day 16-56 and from day 0 to 56 of the experiment. A better feed conversion ratio (FCR) during day 0-56 of the experiment was noted after addition of the probiotic and yeast. The use of YL yeast in diets for piglets had a more beneficial effect on weight gain and FCR than SC. Moreover, the addition of YL modulates lipid metabolism in piglets, as indicated by the reduced levels of TCH, LDL and TG and increased level of HDL in the plasma. The combined use of either YL or SC with a probiotic is not recommended in piglet rearing, as it does not substantially improve growth performance and health in comparison with yeast alone.


Assuntos
Probióticos , Saccharomyces cerevisiae , Suínos/crescimento & desenvolvimento , Yarrowia , Animais , Suplementos Nutricionais , Digestão/fisiologia , Suínos/sangue , Suínos/metabolismo
9.
Folia Med Cracov ; 58(1): 81-95, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30079903

RESUMO

BACKGROUND AND OBJECTIVE: Urological diseases represent a significant health issue worldwide. Presented study aimed at assessing current urological knowledge and confidence in performing urological diagnostic and therapeutic procedures among medical students at Jagiellonian University Medical College in Poland and compare it on different stages of the undergraduate medical education. MATERIAL AND METHODS: We designed an anonymous survey distributed among Polish students from 1st to 6th year of medical studies, before and after clinical urology course. Questions concerned general urological knowledge, prostate diseases, erectile dysfunction, and self-reported practical urological skills. RESULTS: Overall, 437 respondents participated in the survey. Mean total test score in our study group was 50.08%, mean general urological knowledge score was 53.44%, mean prostate diseases knowledge score was 55.43%, mean erectile dysfunction score was 36% and mean practical skills score was 45.83%. Mean total test score increased with consecutive years of studies (R = 0.58; p <0.001). The risk of an above average total test score was significantly influenced by the urology course (OR = 7.95, 95%CI = 1.81-34.84, p = 0.006) and the year of medical studies (4th-6th vs. 1st-3rd) (OR = 5.16, 95%CI = 3.41-7.81, p <0.001). Practical skills score above average was significantly more frequent in the group after the urology course (OR = 6.75, 95%CI = 1.54-29.58, p = 0.011). CONCLUSIONS: Results of this study reveal low mean scores obtained by students, even after completing the urology course, which implies that curriculum requires further development. Urological knowledge and self-assessed practical skills increased with years of medical education. The urology course improved the score obtained in our survey, both in terms of total test score and practical skills.


Assuntos
Competência Clínica/estatística & dados numéricos , Educação Baseada em Competências/estatística & dados numéricos , Educação Médica/organização & administração , Estudantes de Medicina/estatística & dados numéricos , Urologia/educação , Currículo , Avaliação Educacional , Humanos , Masculino , Polônia
10.
Eur J Pharm Biopharm ; 131: 44-47, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30048746

RESUMO

Three-dimensional printing is one of the fastest developing technology within pharmaceutical field. With many advantages this method can be found as a new dosage form manufacturing technique, however low printing efficiency stays as one of the major limitations. Therefore, the preparation of filaments as a feedstock and printing of the final dosage forms in pharmacies may by the direction of development for this method. Thus, simple dosage and dissolution profile modification seems to be essential. This can be done in simple way by addition drug-free filament during printing process. In this work the influence of dual co-extrusion process on the properties of 3D-printed tablets with aripiprazole was evaluated. A ZMorph® 3D printer equipped with DualPro extruder was employed to produce tablets made from Kollicoat® IR aripiprazole-loaded filament and commercially available PLA filament used to modify the release profile. Optical and polarized light microscopy were utilized to evaluate structure of printed objects and X-ray diffraction studies were performed to determine crystallinity of aripiprazole within filament and tablets. Fast dissolution of aripiprazole resulted from its amorphization while prolonged drug release was a result of co-extrusion with PLA filament. Importantly, the drug remained crystalline within the filament and phase transition into disordered system appeared during printing of tablets. Given the high stability of crystalline materials such feature is especially beneficial for long-term storage of feedstock filament.


Assuntos
Aripiprazol/química , Química Farmacêutica/métodos , Impressão Tridimensional , Comprimidos , Aripiprazol/administração & dosagem , Cristalização , Solubilidade , Comprimidos com Revestimento Entérico , Difração de Raios X
11.
Res Vet Sci ; 119: 221-227, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30005396

RESUMO

The objective of the study was to compare the efficiency of two species of yeast, Yarrowia lipolytica (YL) and Saccharomyces cerevisiae (SC), with or without a probiotic supplement, added to feed for piglets, on the basis of haematological blood indices and the gut microbiota. A total of 360 piglets (the average 27-d-old) were allotted to dietary treatments: 1) the basal control(C) diet, 2) the C diet + probiotic(P) (a mixture of Bacillus licheniformis and Bacillus subtilis), 3) the C diet +3%YL(Y), 4) the C diet +3%YL + probiotic(YP), 5) the C diet +3%SC(S) and 6) the C diet +3%SC + probiotic(SP). The study showed that YL yeast can be used in compound feeds for piglets interchangeably with SC yeast. The effect of YL on haematological blood parameters and the microbes colonizing the gut proved to be more beneficial than the effect of SC yeast. The combined application of YL or SC with a probiotic had a more favourable effect on the gut microbiota than the use of yeast alone. It should be noted, however, that supplementation of the compound feed with YL in combination with a probiotic reduced the multiplication of coliform bacteria and Escherichia coli in the intestinal contents, while the feed containing SC together with a probiotic did not. The dietary study confirmed that YL in combination with a probiotic is highly suitable for feeding piglets.


Assuntos
Microbioma Gastrointestinal , Probióticos , Suínos/sangue , Suínos/microbiologia , Ração Animal , Animais , Dieta , Suplementos Nutricionais , Saccharomyces cerevisiae , Yarrowia
12.
World J Urol ; 36(2): 231-240, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29127452

RESUMO

OBJECTIVES: To evaluate the concordance rate of lymphovascular invasion (LVI) and variant histology (VH) of transurethral resection (TUR) with radical cystectomy (RC) specimens. Furthermore, to evaluate the value of LVI and VH at TUR for predicting non-organ confined (NOC) disease, lymph node metastasis, and survival outcomes. PATIENTS AND METHODS: Two hundred and sixty-eight patients who underwent TUR and subsequent RC were reviewed. Logistic regression analyses were performed to evaluate the association of LVI and VH with NOC and lymph node metastasis at RC. Cox regression analyses were used to estimate recurrence-free survival (RFS) and cancer-specific survival (CSS). RESULTS: LVI and VH were detected in 13.8 and 11.2% of TUR specimens, and in 30.2 and 25.4% of RC specimens, respectively. The concordance rate between LVI and VH at TUR and subsequent RC was 69.8 and 83.6%, respectively. They were both associated with adverse pathological features such as lymph node metastasis and advanced stage. TUR LVI and VH were both independently associated with lymph node metastasis and TUR VH was independently associated with NOC. On univariable Cox regression analyses, TUR LVI was associated with RFS and CSS while TUR VH was only associated with RFS. Only TUR LVI was independently associated with RFS. CONCLUSION: Detection of LVI is missed in a third of TUR specimens while VH seems more accurately identified. TUR LVI and VH are associated with more advanced disease and LVI predicts disease recurrence. Assessment and reporting of LVI and VH on TUR specimen are important for risk stratification and decision-making.


Assuntos
Carcinoma de Células de Transição/patologia , Neoplasias da Bexiga Urinária/patologia , Idoso , Carcinoma de Células de Transição/mortalidade , Carcinoma de Células de Transição/cirurgia , Tomada de Decisão Clínica , Cistectomia , Cistoscopia , Intervalo Livre de Doença , Feminino , Humanos , Modelos Logísticos , Linfonodos/patologia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Medição de Risco , Neoplasias da Bexiga Urinária/mortalidade , Neoplasias da Bexiga Urinária/cirurgia
13.
Urol Oncol ; 36(4): 158.e1-158.e6, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29288003

RESUMO

AIM: To assess the predictive value of TGP on biochemical recurrence (BCR) and its association with clinicopathological outcomes in a large, multicenter cohort of patients with localized prostate cancer (PCa) treated with radical prostatectomy (RP). MATERIALS AND METHODS: Records of 6,041 patients who were treated with RP between 2000 and 2011 for clinically nonmetastatic PCa were, retrospectively, analyzed from prospectively collected datasets. BCR-free survival rates were assessed using univariable and multivariable cox-regression analyses. RESULTS: Median patient age was 61 years (interquartile range [IQR]: 57-66) with a median preoperative prostrate specific antigen of 6ng/ml (IQR: 4-9). Overall, 28% of patients had Gleason score (GS) 6, 0.3% GS 6 + TGP, 33% GS 7 (3 + 4), 0.2% GS 7 (3 + 4) + TGP, 22% GS 7 (4 + 3), 0.2% GS 7 (4 + 3) + TGP, 0.1% GS 8 and 0.4% GS 9 or 10. Median follow-up was 45 months (IQR: 31-57). Harboring a TGP was associated with higher rates of positive surgical margins, lymphovascular invasion, extraprostatic extension, and seminal vesicle invasion than their counterparts within the same GS group as well as in the next higher GS group (all P ≤ 0.05). At 5 years post-RP, BCR estimates were 5% for patients with GS 6, 13% for patients with GS 6 + TGP, 6% for patients with GS 7 (3 + 4), 22% for patients with GS 7 (3 + 4) + TGP, 16% for patients with GS 7 (4 + 3), 41% for patients with GS 7 (4 + 3) + TGP, 38% for patients with GS 8 (4 + 4) and 46% for patients with GS 9 or 10. Patients harboring a TGP had higher BCR rates than the patients in the next higher GS group: GS 6 + TGP vs. GS 7 (3 + 4), HR = 1.6, P = 0.02 and GS 7 (3 + 4)+TGP vs. GS 7 (4 + 3), HR = 1.4, P = 0.03. Patients with a TGP in the GS 7 (4 + 3) group had comparable BCR rates as patients with GS = 8 (P = 0.4) and GS 9 to 10 (P = 0.2). On multivariable analysis that adjusted for the effects of preoperative prostrate specific antigen, nodal involvement, positive surgical margin, extraprostatic disease (pT3a), seminal vesicle invasion (pT3b) and different institution, harboring a TGP showed higher risk of developing BCR within the same GS group and comparable risk of developing BCR with the next higher GS group. CONCLUSION: Patients with TGP at RP have adverse clinicopathological features when compared to their counterparts in the same and the next higher GS group without TGP. Risk of developing BCR increases with the presence of TGP within the same GS group. This risk seems to be comparable between patients with TGP and their counterparts in the next higher GS group without TGP. Knowledge of TGP in RP specimens is likely to improve risk stratification, patient counseling and follow-up scheduling. Further prospective studies that control significant clinical endpoints such as metastasis and mortality are necessary for more significant predictions.


Assuntos
Recidiva Local de Neoplasia/epidemiologia , Próstata/patologia , Prostatectomia , Neoplasias da Próstata/patologia , Idoso , Intervalo Livre de Doença , Seguimentos , Humanos , Calicreínas/sangue , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Recidiva Local de Neoplasia/sangue , Estudos Prospectivos , Próstata/cirurgia , Antígeno Prostático Específico/sangue , Neoplasias da Próstata/sangue , Neoplasias da Próstata/epidemiologia , Neoplasias da Próstata/cirurgia , Estudos Retrospectivos , Resultado do Tratamento
14.
Arch Anim Nutr ; 71(5): 347-361, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28737427

RESUMO

The hypothesis postulating that the antioxidant and immunological effects of dietary methionine (Met) in young turkeys (1-8 weeks of age) can be differentiated by level and source of Met was investigated in this study. A total of 544 female Hybrid Converter turkeys were divided into four groups and fed diets in which Met content was tailored through supplementation with dl-methionine (dl-Met) or dl-methionine hydroxy analogue (MHA) to levels recommended by NRC (1994) (Groups dl-MetL and MHAL) and exceeding them by 50% (Groups dl-MetH and MHAH). Regardless of its source, the increased dietary Met content led to significantly higher body weight gains but had no effect on feed conversion rates. Moreover, an increased Met content lowered lipid peroxide concentrations in breast meat and increased selected indicators of the plasma antioxidant status like uric acid levels, activity of superoxide dismutase (SOD), glutathione (GSH) concentrations, the ferric-reducing ability of plasma (FRAP), increased immunoglobulin A (IgA) plasma levels and decreased interleukin 6 levels. In comparison with dl-Met, MHA decreased the activities of SOD and catalase, and GSH concentrations in plasma. A dosage by source interaction revealed that the lower MHA content was associated with the lowest plasma GSH concentrations, FRAP values and activities of SOD and catalase. The higher dietary MHA level resulted for most parameters similar values, except for a decrease in lipid peroxide concentrations and an increase in plasma IgA levels. It can be concluded that an increased dietary dl-Met and MHA content (about 150% of the recommendations given by NRC 1994) not only increased the growth rate of young turkeys but also improved their antioxidant status. MHA appears to be a less desirable source of dietary Met for young turkeys when the inclusion level of Met matches the current recommendations. Therefore, a further debate is needed to establish the dietary requirements for Met in poultry.


Assuntos
Antioxidantes/metabolismo , Suplementos Nutricionais , Imunidade Inata , Metionina/análogos & derivados , Metionina/administração & dosagem , Perus/imunologia , Perus/metabolismo , Ração Animal/análise , Animais , Dieta/veterinária , Feminino
15.
Arch Anim Nutr ; 70(2): 127-40, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26829577

RESUMO

A total of 490 eight-week-old female Hybrid Converter turkeys (body weight 4.11 ± 0.03 kg) were divided into 5 groups with 7 replicates of 14 birds each. For 8 weeks, basal diets were supplemented with methionine (Met) at following levels (weeks 9-12/weeks 13-16 of age): Group 1 - 0.34/0.29%, Group 2 - 0.39/0.34%, Groups 3 and 4 - 0.45/0.38% and 0.51/0.41%, respectively, Group 5 - 0.58/0.47%. Only in the first feeding phase the body weight gain (BWG) was affected by Met levels with the significantly highest BWG in Group 3. No treatment effects were found for feed conversion ratio, carcass yield, carcass composition and meat colour. The blood superoxide dismutase activity was significantly highest in Groups 2 and 3. The concentrations of reduced glutathione in the liver were linearly increased (p = 0.018), whereas the ratio of reduced glutathione to oxidised glutathione was highest in Group 3 (quadratic contrast, p = 0.004). It can be concluded that turkeys from Group 3 (Met levels age depending 15% and 10% above recommendations by NRC) were characterised by a well-balanced physiological response. Attention should be paid to the immune response of birds to higher dietary Met levels: plasma IgA concentrations decreased, whereas IL-6 and TNF-α levels increased in turkeys fed diets with the highest Met content.


Assuntos
Ração Animal/análise , Dieta/veterinária , Metionina/farmacologia , Perus/crescimento & desenvolvimento , Ganho de Peso/efeitos dos fármacos , Fenômenos Fisiológicos da Nutrição Animal , Animais , Composição Corporal/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Metionina/administração & dosagem , Músculo Esquelético/crescimento & desenvolvimento , Perus/sangue
16.
Przegl Lek ; 70(4): 195-8, 2013.
Artigo em Polonês | MEDLINE | ID: mdl-23991557

RESUMO

Peyronie's disease (lat. induratio penis plastica) is a process of the fibrotic plaques oand other localized fibrotic conditions have been considered to be the result of an abnormal size, pain and improved penile curvature. At early stages intralesional injections may decrease penile curvature and decrease plaque volume although the exact mechanism of action on Peyronie disease is unknown. In serious cases surgery is recommended, based on ultrasound examination, cavernosography and cavernosometry. There are three mail surgical procedures to correct the curvature in Peyronie's disease: Nesbit plication, plaque excision followed by skin grafting, another autograft or synthetic material, and implantation of a penile prosthesis Aim of this study is to present our experience in surgical treatment of severe stadium in Peyronie's disease. Peyronie plaque was excised in 8 man, previously potent with severe satium of the disease. In every case saphenous autograft replacing excised plaque was used. In every case was not intra and postoperative complications. All patients reported satisfactory cosmetic and functional result. The satisfactory result of the treatment of severe stadium Peyronie disease is based on the surgical method. Saphenous graft is effective, safe and successful technique in our knowledge.


Assuntos
Induração Peniana/cirurgia , Idoso , Disfunção Erétil/diagnóstico por imagem , Disfunção Erétil/etiologia , Disfunção Erétil/terapia , Humanos , Masculino , Pessoa de Meia-Idade , Induração Peniana/complicações , Induração Peniana/diagnóstico por imagem , Prótese de Pênis , Pênis/diagnóstico por imagem , Pênis/cirurgia , Transplante de Pele/métodos , Ultrassonografia
17.
Przegl Lek ; 70(1): 6-10, 2013.
Artigo em Polonês | MEDLINE | ID: mdl-23789297

RESUMO

BACKGROUND: The frequency of arterial hypertension (AHT) in children is around 3.2-4.5% nowadays, however 3-4 decades ago it was below 1.5%. In obese children AHT is 3-5 times more frequent than in lean children. AIM: Retrospective evaluation of frequency of AHT and metabolic complications in obese children and adolescents. MATERIAL AND METHODS: One hundred seventy children and adolescents (100 girls) at the mean age of 14.6 years (range: 10-18 years) diagnosed with simple obesity admitted to The Department of Pediatric and Adolescent Endocrinology in Krakow, Poland were assessed in the study. Patients were divided into three groups: 1- obese normotensive (n=43), 2- obese prehypertensive (n=31), 3- obese hypertensive (n=96). Blood pressure (BP) was assessed by Korotkow method. The result was expressed as a mean of 3 independent BP measurements. Biochemical analysis included oral glucose tolerance test with assessments of glucose, insulin and HOMA-IR (the homeostatic index for insulin resistance) as well as lipid profile and uric acid measurements. RESULTS: AHT was found in 96 patients (55.8%), in 53 girls (52%) and 43 boys (61.4%). Prehypertensive values of BP were found in 31 patients (18 girls). Systolic AHT was found in 90 patients (52.9%), diastolic AHT in 56 patients (32.9%), and both systolic and diastolic AHT in 50 patients (29.4%). In group 3. BMI was higher than in group 1. BMI correlated significantly with systolic and diastolic BP. Uric acid levels were higher in group 3. than in group 1., and higher in patients with diastolic AHT. Basal insulin levels were higher in diastolic AHT patients and poststimulatory insulin was higher in systolic AHT patients. HOMA-IR was higher in patients with diastolic AHT than in patients with normal diastolic BP. Lipid profile did not differ between the groups, however the highest percentage of patients with abnormal lipid profile was found in group 2. CONCLUSION: An early endocrine referral in pediatric obese patients is advisable to monitor complications of obesity.


Assuntos
Hipertensão/epidemiologia , Obesidade/epidemiologia , Adolescente , Índice de Massa Corporal , Criança , Comorbidade , Diástole , Feminino , Humanos , Incidência , Resistência à Insulina , Masculino , Estudos Retrospectivos , Sístole
18.
Arch Immunol Ther Exp (Warsz) ; 61(3): 245-53, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23456207

RESUMO

Glucose metabolism disorders influence anticarcinogenic function of natural killer (NK) cells. The aim of this study was to evaluate the number and cytotoxic activity of NK cells in type 2 diabetic (T2D) patients with negative family history of cancer, type 2 diabetic subjects with newly diagnosed untreated colon cancer (T2DCC) and patients without type 2 diabetes with newly diagnosed, untreated colon cancer (CC). Incubation tests were performed in 18 T2D patients, treated with diet and oral antidiabetic agents, 16 T2DCC; cT1-4N0M0 (c-clinical diagnosis based on computed tomography, colonoscopy and histopathology) treated with diet and oral antidiabetic agents and 16 normoglycemic CC; cT1-4N0M0. Control group included 18 metabolically healthy (with normal fasting glucose and normal glucose tolerance) subjects (HS) with negative family history of cancer, matched by age, BMI and waist circumference. Peripheral blood mononuclear cells were isolated by means of gradient centrifugation. The K562 human erythroleukemia cell line served as the standard target for human NK cytotoxicity assay. The T2D revealed an increased number of NK cells (13.56 ± 5.9 vs 9.50 ± 4.8 %; p < 0.05) when compared with HS, yet these cells had a decreased activity (3.3 ± 2.5 vs 9.4 ± 3.6 %; p < 0.01). The CC demonstrated a decreased activity (2.9 ± 1.8 %; p < 0.01) but a similar number (8.82 ± 3.7 %; not significant) of NK cells when compared to HS. The T2DCC NK cells were characterized by trace cytotoxic activity (1.1 ± 0.7 %; p < 0.01) and nearly three times greater amount (21.24 ± 7.5 %; p < 0.01) when compared to T2D. Type 2 diabetes and CC are associated with disadvantageous alterations of NK cells, leading to impairment in their cytotoxic activity. The impaired activity of NK cells in T2D can be involved in the increased carcinogenic risk and can promote a higher incidence of CC.


Assuntos
Neoplasias do Colo/imunologia , Citotoxicidade Imunológica , Diabetes Mellitus Tipo 2/imunologia , Células Matadoras Naturais/imunologia , Administração Oral , Biomarcadores/sangue , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Estudos de Casos e Controles , Técnicas de Cocultura , Neoplasias do Colo/diagnóstico , Neoplasias do Colo/epidemiologia , Colonoscopia , Testes Imunológicos de Citotoxicidade , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/dietoterapia , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/epidemiologia , Feminino , Hemoglobina A Glicada/metabolismo , Humanos , Hipoglicemiantes/administração & dosagem , Incidência , Insulina/sangue , Células K562 , Contagem de Linfócitos , Masculino , Pessoa de Meia-Idade , Polônia/epidemiologia , Tomografia Computadorizada por Raios X
19.
Endokrynol Pol ; 63(3): 212-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22744628

RESUMO

INTRODUCTION: The proper expression of particular glucotransporter (GLUT) isoforms determines a sufficient supply of glucose to tissues. The impairment of cellular glucose transport observed in insulin resistance leads to glucose metabolism disturbances. The aim of this study was the estimation of insulin resistance indicators and the quantitative expression of GLUT-1, GLUT-3 and GLUT-4 on peripheral blood lymphocytes in prediabetic subjects and persons with a positive family history of type 2 diabetes during 24 months of observation. MATERIAL AND METHODS: The study included 25 prediabetic subjects (according to WHO criteria) and 24 normoglycaemic individuals with a positive family history of type 2 diabetes. Twenty three healthy subjects with no family history of type 2 diabetes, matched with BMI, served as a control group. All participants were recommended to perform physical activity for at least 140 minutes per week and to maintain a low calorie diet. The peripheral blood lymphocytes demonstrating expression of GLUT-1, GLUT-3 and GLUT-4 were labelled with the use of indirect immunofluorescence. The expression of GLUT isoforms was investigated by flow cytometry. Cells were stained by using anti-human GLUT antibodies and FITC-conjugated immunoglobulin. Flow cytometry was performed using a FACS Calibur (Becton-Dickinson). Additionally, we determined: fasting plasma glucose (FPG), insulin and C peptide concentrations, HOMA-IR, BMI and WHR. All the tests were performed at baseline, and after 12 and 24 months. RESULTS: At baseline, prediabetics and subjects with a positive family history of type 2 diabetes were characterised by a much higher expression of GLUT-4 compared to control subjects. Twenty four months of lifestyle modification resulted in significant lowering of the expression of GLUT-4 on the surface of PBL in both studied groups, with no differences in the expression of GLUT-1 or GLUT-3. Both prediabetic subjects and individuals with a positive family history of type 2 diabetes revealed no significant differences in determined insulin resistance markers after 24 months of the observation compared to the baseline values. CONCLUSIONS: The estimation of typical GLUT isoforms present on the peripheral blood lymphocytes, as well as the evaluation of insulin resistance indicators, are obviously insufficient for monitoring the metabolic disorders progression in the risk groups of type 2 diabetes. The decrease in GLUT-4 lymphocyte expression may reflect a positive influence of lifestyle modification on a tissue redistribution of this crucial insulin-dependent glucotransporter. The determination of GLUT-4 on the surface of peripheral blood lymphocytes can be a useful tool for the evaluation of the efficacy of therapeutic actions in subjects at high risk of type 2 diabetes.


Assuntos
Diabetes Mellitus Tipo 2/metabolismo , Proteínas Facilitadoras de Transporte de Glucose/metabolismo , Resistência à Insulina/fisiologia , Estado Pré-Diabético/metabolismo , Adulto , Glicemia/genética , Glicemia/metabolismo , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/genética , Feminino , Seguimentos , Predisposição Genética para Doença , Proteínas Facilitadoras de Transporte de Glucose/genética , Proteínas Facilitadoras de Transporte de Glucose/fisiologia , Humanos , Resistência à Insulina/genética , Masculino , Pessoa de Meia-Idade , Linhagem , Estado Pré-Diabético/genética , Fatores de Risco
20.
Arch Immunol Ther Exp (Warsz) ; 59(3): 215-30, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21448680

RESUMO

Diabetes and cancer are diseases which take the size of an epidemic spread across the globe. Those diseases are influenced by many factors, both genetic and environmental. Precise knowledge of the complex relationships and interactions between these two conditions is of great importance for their prevention and treatment. Many epidemiological studies have shown that certain types of cancer, especially gastrointestinal cancers (pancreas, liver, colon) and also the urinary and reproductive system cancers in women are more common in patients with diabetes or related metabolic disorders. There are also studies showing the inverse relationship between diabetes and cancer, or the lack of it, but they are less numerous and relate mainly to prostate cancer or squamous cell carcinoma of the esophagus. Epidemiological studies, however, do not say anything about the mechanisms of these dependencies. For this purpose, molecular research is needed on the metabolism of cells (including tumor cells) and on metabolic dysfunctions that arise due to changes in the cell environment taking place in the sick, as well as in the intensely treated human organism.


Assuntos
Diabetes Mellitus/epidemiologia , Neoplasias Gastrointestinais/epidemiologia , Transtornos do Metabolismo de Glucose/epidemiologia , Glucose/metabolismo , Animais , Diabetes Mellitus/fisiopatologia , Neoplasias Gastrointestinais/fisiopatologia , Transtornos do Metabolismo de Glucose/fisiopatologia , Humanos , Hiperglicemia , Hiperinsulinismo , Inflamação , Risco
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