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1.
J Asian Nat Prod Res ; : 1-10, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31791147

RESUMO

Various bioactive polyketides have been found in Aloe barbadensis. However, the polyketide synthases (PKSs), which participate in biosynthesis of polyketides in A. barbadensis remain unknown. In this study, two type III PKSs (AbPKS1 and AbPKS2) were identified from A. barbadensis. AbPKS1 and AbPKS2 were able to utilize malonyl-CoA to yield heptaketides (TW93a and aloesone) and octaketides (SEK4 and SEK4b), respectively. AbPKS1 also exhibited catalytic promiscuity in recognizing CoA thioesters of aromatics to produce unusual polyketides. What Is more, a whole cell biocatalysis system with the capability of producing 26.4 mg/L of SEK4/SEK4b and 2.1 mg/L of aloesone was successfully established.

2.
J Asian Nat Prod Res ; : 1-7, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31738087

RESUMO

Bistachybotrysin K (1), one new phenylspirodrimane dimer with a central 6/7 oxygen heterocycle core, was isolated from the fungus Stachybotrys chartarum CGMCC 3.5365. Its structure was elucidated by extensive spectroscopic data and single-crystal X-ray diffraction. Compound 1 showed significant cytotoxicity against human tumor cell lines HCT116, NCI-H460, BGC823, Daoy, and HepG2 with IC50 values in the range of 1.1-4.7 µM.

3.
J Asian Nat Prod Res ; 21(9): 887-894, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30614271

RESUMO

Three new phenylspirodrimanes derivatives named stachybotrysins H and I (1 and 2) and stachybotrin E (3), together with one known compound stachybotrylactam (4), were isolated from Stachybotrys chartarum CGMCC 3.5365. Their structures were determined by extensive NMR data and mass spectroscopic analysis. Compounds 1 and 2 showed inhibitory effect towards potassium channel Kv1.3 with IC50 values of 13.4 and 10.9 µM, respectively.


Assuntos
Canal de Potássio Kv1.3/antagonistas & inibidores , Compostos de Espiro/química , Stachybotrys/química , Animais , Células CHO , Linhagem Celular , Cricetinae , Cricetulus
4.
J Asian Nat Prod Res ; 20(7): 605-614, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29989425

RESUMO

Glucuronidation is an important and popular metabolic reaction in vivo of drugs. The further evaluation of biological activity and toxicity of glucuronides is necessary in the course of the drug research and development. However, the synthesis of glucuronides is limited by the lack of efficient approach. Herein, we have developed a new glucuronide synthesis method using plant uridine diphosphate-dependent glucuronosyltransferases (UGTs), UGT88D4, UGT88D7, and EpGT8, enabling the convenient preparation for corresponding O-glucuronide metabolites (1a, 2a, 3a, and 3b) in milligram scale of two neurological active agents, IMM-H004 (1) and FLZ (2). Their structures were characterized by spectroscopic data analyses.


Assuntos
Glucuronídeos/síntese química , Glucuronosiltransferase/metabolismo , Plantas/enzimologia , Clonagem Molecular , Glucuronosiltransferase/química , Glucuronosiltransferase/genética , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética , Metais/química , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray , Temperatura Ambiente
5.
Fitoterapia ; 127: 322-327, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29544761

RESUMO

Seven new phenolic bisabolane sesquiterpenoids, (7R,10S)-7,10-epoxysydonic acid (1), (7S,10S)-7,10-epoxysydonic acid (2), (7R,11S)-7,12-epoxysydonic acid (3), (7S,11S)-7,12-epoxysydonic acid (4), 7-deoxy-7,14-didehydro-12-hydroxysydonic acid (5), (Z)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (6), and (E)-7-deoxy-7,8-didehydro-12-hydroxysydonic acid (7), along with five known analogues (8-12), were obtained from the culture of an endophytic fungus Aspergillus sp. xy02 isolated from the leaves of a Thai mangrove Xylocarpus moluccensis. All structures were assigned on the basis of detailed spectroscopic analyses. The absolute configurations of 1-4, being two pairs of epimers, were established by TDDFT-ECD calculations. Compound 12 showed mild antioxidative activity to scavenge DPPH radical with an IC50 of 72.1 µM, whereas 2, 3, 5, 7, 9, 11, and 12 displayed moderate inhibitory activities against Staphylococcus aureus ATCC 25923 with IC50 values ranging from 31.5 to 41.9 µM.


Assuntos
Aspergillus/química , Fenóis/isolamento & purificação , Sesquiterpenos/isolamento & purificação , Antibacterianos/isolamento & purificação , Depuradores de Radicais Livres/isolamento & purificação , Meliaceae/microbiologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Folhas de Planta/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Tailândia
6.
Eur J Med Chem ; 145: 717-725, 2018 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-29353723

RESUMO

Bysspectin A (1), a polyketide-derived octaketide dimer with a novel carbon skeleton, and two new precursor derivatives, bysspectins B and C (2 and 3), were obtained from an organic extract of the endophytic fungus Byssochlamys spectabilis that had been isolated from a leaf tissue of the traditional Chinese medicinal plant Edgeworthia chrysantha, together with a known octaketide, paecilocin A (4). Their structures were determined by HRMS, 1D and 2D NMR spectroscopic analysis. A plausible route for their biosynthetic pathway is proposed. Compounds 1-3 were tested for their antimicrobial activities. Only compound 3 was weakly active against Escherichia coli and Staphyloccocus aureus with MIC values of 32 and 64 µg/mL, respectively. Further, the inhibitory effects on human carboxylesterases (hCE1, hCE2) of compounds 1 and 4 were evaluated. The results demonstrated that bysspectin A (1) was a novel and highly selective inhibitor against hCE2 with the IC50 value of 2.01 µM. Docking simulation also demonstrated that active compound 1 created interaction with the Ser-288 (the catalytic amino-acid in the catalytic cavity) of hCE2 via hydrogen bonding, revealing its highly selective inhibition toward hCE2.


Assuntos
Antibacterianos/farmacologia , Byssochlamys/química , Carboxilesterase/antagonistas & inibidores , Hidrolases de Éster Carboxílico/antagonistas & inibidores , Inibidores Enzimáticos/farmacologia , Escherichia coli/efeitos dos fármacos , Policetídeos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/isolamento & purificação , Biocatálise , Carboxilesterase/metabolismo , Hidrolases de Éster Carboxílico/metabolismo , Dimerização , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Humanos , Testes de Sensibilidade Microbiana , Simulação de Acoplamento Molecular , Estrutura Molecular , Policetídeos/química , Policetídeos/isolamento & purificação , Relação Estrutura-Atividade
7.
J Asian Nat Prod Res ; 20(9): 844-851, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29119831

RESUMO

Two new lanostane triterpenoids (1 and 2), two new ergostane-type steroids (3 and 4) together with two known lanostane triterpenoids (5 and 6) and one known steroid (7) were isolated from the cultured mycelia of Ganoderma capense (CGMCC 5.71). Their structures were determined on the basis of extensive spectroscopic (HRESIMS, 1D NMR, 2D NMR) data analyses. Compound 1 exhibited moderate cytotoxic activity against the human cancer cell line NCI-H1650 with an IC50 value of 22.3 µM, and 7 displayed cytotoxic activity against the human cancer cell line HCT116 with an IC50 value of 17.4 µM. In addition, compounds 2, 3, 5, and 6 displayed weak anti-HIV activity with IC50 values of 23.5, 46.7, 21.6, and 30.1 µM, respectively.


Assuntos
Ganoderma/química , Micélio/química , Esteroides/química , Triterpenos/química , Ganoderma/metabolismo , Estrutura Molecular , Micélio/metabolismo , Esteroides/metabolismo
8.
J Asian Nat Prod Res ; 20(3): 217-226, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28581824

RESUMO

Three new xanthone dimers, named phomoxanthones C-E (1-3), were obtained from the Thai mangrove fungus Phomopsis sp. xy21, together with four known ones. The structures of these compounds were elucidated by the analysis of HRESIMS and extensive NMR spectroscopic data. The absolute configuration of 1 was established by the analysis of single-crystal X-ray diffraction with Cu Kα radiation. Phomoxanthones C (1) and D (2) possess a highly oxidized hexahydroxanthone skeleton.


Assuntos
Ascomicetos/química , Xantonas/isolamento & purificação , Cristalografia por Raios X , Meliaceae/microbiologia , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Rhizophoraceae/microbiologia , Tailândia , Xantonas/química
9.
Zhongguo Zhong Yao Za Zhi ; 42(12): 2323-2328, 2017 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-28822187

RESUMO

The glycosides of 4'-demethylepipodophyllotoxin (DMEP) possess various pharmacological activities; however, the chemical synthesis of these glycosides faces challenges in regioselectivity, stereoselectivity, and the protection and de-protection of functional groups. In this work, a novel glycosyltransferase (GT) gene AbGT5 from Aloe barbadensis was successfully cloned, heterogeneously expressed and purified. Recombinant AbGT5 was able to catalyze the glycosylation of DMEP and the glycosylated product, which was separated from the preparative scale reaction, was characterized as DMEP 4'-O-ß-D-glucoside via MS, 1H-NMR, 13C-NMR, HSQC and HMBC. According to the investigations of enzyme properties, AbGT5 show the highest activity around 20 ℃ in the buffer of pH 9.0, and it was independent of divalent metal ions. Under the optimum conditions, the conversion rate of DMEP can reach 80%. Above all, in this work the enzymatic glycosylation of DMEP was achieved with high efficiency by the novel GT AbGT5.


Assuntos
Glucosídeos/química , Glicosídeos/química , Glicosiltransferases/metabolismo , Podofilotoxina/análogos & derivados , Aloe/enzimologia , Aloe/genética , Glicosilação , Glicosiltransferases/genética , Podofilotoxina/química
10.
J Asian Nat Prod Res ; 19(6): 541-549, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28395517

RESUMO

Five monoterpenoids were isolated from the endophytic fungus Periconia sp. F-31, including three new carene-type monoterpenoids, 2-carene-5,8-diol (1), 2-carene-8,10-diol (2), 2-carene-8-acetamide (3), one new menthene-type monoterpenoid 8-hydroxy-1,7-expoxy-2-menthene (4), and one known monoterpenoid anethofuran (5). The structures of all compounds were elucidated based on a comprehensive spectroscopic data analysis, electronic circular dichroism (ECD), and calculated ECD.


Assuntos
Antineoplásicos/isolamento & purificação , Ascomicetos/química , Monoterpenos/isolamento & purificação , Antineoplásicos/química , Antineoplásicos/farmacologia , Dicroísmo Circular , Cristalografia por Raios X , Ensaios de Seleção de Medicamentos Antitumorais , Estrutura Molecular , Monoterpenos/química , Monoterpenos/farmacologia , Ressonância Magnética Nuclear Biomolecular
11.
J Asian Nat Prod Res ; 19(10): 1028-1035, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28145126

RESUMO

A new steroid glucoside (1), along with nine known steroids (2-10) and four known sorbicillinoids (11-14), were isolated from the endophytic fungus Trichoderma sp. Xy24. Their structures were elucidated on the basis of spectroscopic data analyses and by comparison with reported data. Compounds 3, 5-7, 9, 10, and 13 exhibited significant inhibitory effects on HIV-1 virus with IC50 values ranging 1.9-9.3 µM; compounds 10, 13, and 14 showed potent inhibitory activity on LPS-induced NO production in BV2 microglia cells with inhibitory rates of 108.2, 100, and 75.1% at 10 µM, respectively. In addition, compound 10 displayed moderate cytotoxicity against BCG823 and HePG2 cell lines with IC50 values of 11.1 and 17.7 µM, respectively.


Assuntos
Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Esteroides/isolamento & purificação , Esteroides/farmacologia , Trichoderma/química , Anti-Inflamatórios não Esteroides/farmacologia , Glucosídeos/química , Células HCT116 , HIV-1/efeitos dos fármacos , Células Hep G2 , Humanos , Concentração Inibidora 50 , Microglia/efeitos dos fármacos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Paclitaxel/farmacologia , Esteroides/química
12.
J Asian Nat Prod Res ; 19(7): 651-658, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27835936

RESUMO

Three new sesquiterpenoids trichoacorenols B-C and cyclonerodiol B (1-3), along with three known ones (4-6), were isolated from the mangrove plant endophytic fungus Trichoderma sp. Xy24 using various column chromatography techniques. The structures of these compounds were determined on the basis of extensive spectroscopic data analyses. Compounds 1, 2, 4, and 5 were four acorane sesquiterpenes, 3 and 6 were two monocyclic sesquiterpenediols. Compounds 3 and 5 exhibited significant neural anti-inflammatory activity by inhibiting LPS-induced NO production in BV2 cells with the inhibitory rates of 75.0% and 39.2% at 0.1 µM, respectively, which are more potent than curcumin, a positive control with the inhibitory rate of 21.1% at 0.1 µM.


Assuntos
Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/farmacologia , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/farmacologia , Trichoderma/química , Animais , Anti-Inflamatórios/química , Curcumina/farmacologia , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Camundongos , Estrutura Molecular , Óxido Nítrico/biossíntese , Rhizophoraceae/microbiologia , Sesquiterpenos/química
13.
Zhongguo Zhong Yao Za Zhi ; 41(1): 87-91, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-28845646

RESUMO

Various chromatographic techniques, including silica gel column chromatography, Sephadex LH-20, preparative thin-layer chromatography, and preparative HPLC, were employed to isolate the chemical constituents from callus cultures of Dysosma versipellis. Structures of the compounds were elucidated based on UV, IR, MS and NMR spectroscopic data analysis. Totally, seven flavonoid glycosides were isolated from the 95% ethanol extract of the callus cultures and identified as kaempferol-3-O-[6″-(3″'-methoxy)-malonyl]-ß-D-glucopyranoside(1), kaempferol-3-O-(6″-O-acetyl)-ß-D-glucopyranoside(2), kaempferide-3-O-ß-D-glucopyranoside(3), kaempferol-3-O-ß-D-glucopyranoside(4), isoquercitrin(5), quercetin-4'-O-ß-D-glucopyranoside(6) and kaempferol-3-(6″-malonyl)-ß-D-glucopyranoside(7), respectively.All these compounds were isolated from callus cultures of D. versipellis for the first time.Compounds 1, 2, 3, 6 and 7 were firstly obtained from plant materials of D. versipellis, and compound 1 was a new compound.


Assuntos
Berberidaceae/química , Flavonoides/análise , Flavonoides/isolamento & purificação , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray
14.
J Asian Nat Prod Res ; 18(3): 253-9, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26465203

RESUMO

Two new flavonoids (1 and 2), along with 14 known ones (3-16), were isolated from the cultured cells of Glycyrrhiza uralensis. Most of them were prenylated flavonoids. Their structures were elucidated on the basis of spectroscopic data analysis. All compounds showed non-cytotoxicity against five human tumor cell lines. The results suggest that plant cultured cells can yield the secondary metabolites that have not been found in parent plant.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Flavonoides/isolamento & purificação , Glycyrrhiza uralensis/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Células Cultivadas , Ensaios de Seleção de Medicamentos Antitumorais , Flavonoides/química , Flavonoides/farmacologia , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular
15.
Zhongguo Zhong Yao Za Zhi ; 41(24): 4568-4571, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-28936839

RESUMO

Secoisolariciresinol dehydrogenase (SDH) is a key enzyme involved in the biosynthetic pathway of podophyllotoxin.In this study, two SDH candidate genes,SO282 and SO1223, were cloned from callus of Dysosma versipellis by homology-based PCR and rapid amplification of cDNA end (RACE).The SDH candidate genes were expressed in Escherichia coli and the subsequent enzyme assay in vitro showed that recombinant SO282 had the SDH activity. These results pave the way to the follow-up investigation of the biosynthetic of podophyllotoxin.


Assuntos
Berberidaceae/enzimologia , Álcool Oxidorredutases Dependentes de NAD(+) e NADP(+)/genética , Proteínas de Plantas/genética , Berberidaceae/genética , Clonagem Molecular , DNA Complementar , Podofilotoxina/biossíntese
16.
Zhongguo Zhong Yao Za Zhi ; 40(12): 2349-51, 2015 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-26591523

RESUMO

To investigate the secondary metabolites of endophytic fungi Pericinia sp. F-31. Column chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC were used to separate and purify the compounds. Two compounds were isolated from the fermentation broth of Periconia sp. Their structures were identified as 5-(1-hydroxyhexyl) -6-methyl-2H-pyran-2-one (1) and 2-(3-hydroxy-4-methylphenyl) -propanoic acid (2). Compound 1 was a new lactone compound, compound 2 was new natural product, and the NMR data of compound 2 was reported for the first time.


Assuntos
Annona/microbiologia , Ascomicetos/metabolismo , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Endófitos/metabolismo , Lactonas/química , Lactonas/isolamento & purificação , Ascomicetos/química , Ascomicetos/genética , Ascomicetos/isolamento & purificação , Medicamentos de Ervas Chinesas/metabolismo , Endófitos/química , Endófitos/genética , Endófitos/isolamento & purificação , Lactonas/metabolismo , Espectrometria de Massas , Estrutura Molecular
17.
J Asian Nat Prod Res ; 17(6): 683-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26027676

RESUMO

A new 2-arylbenzofuran compound, 5-dehydroxy-moracin U (1), along with 10 known compounds (2-11), were isolated from cell cultures of Morus alba. Their structures were elucidated on the basis of extensive spectroscopic analyses. The anti-inflammatory activity assay of 1-8 showed that 2 and 8 exhibited significant inhibitory effect on LPS-induced NO production with the values of 76.4% and 98.7% at 10(- 5) M, respectively.


Assuntos
Benzofuranos/isolamento & purificação , Morus/química , Anti-Inflamatórios , Benzofuranos/química , Benzofuranos/farmacologia , Lipopolissacarídeos/farmacologia , Estrutura Molecular , Óxido Nítrico/biossíntese , Casca de Planta/química , Extratos Vegetais/química
18.
J Asian Nat Prod Res ; 17(6): 656-61, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26074011

RESUMO

Biotransformations of icariin (1) by cell suspension cultures of Glycyrrhiza uralensis and Morus alba yielded two new metabolites, icaruralins A and B (2 and 3), and one known metabolite, baohuoside I (4). Their structures were determined on the basis of extensive spectroscopic analysis. This is the first report that the cell suspension cultures of G. uralensis and M. alba possess deglycosylation functionality.


Assuntos
Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Glycyrrhiza uralensis/química , Morus/química , Biotransformação , Flavonoides/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química
19.
Zhongguo Zhong Yao Za Zhi ; 40(21): 4212-7, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-27071259

RESUMO

A study on the microbial transformation of glycyrrhetinic acid (GA) was conducted by a fungus, Cunninghamella blakesleeana CGMCC 3.970 systematically. After incubation with the cell cultures of C. blakesleeana CGMCC 3.970 at 25 degrees C for 7 days on a rotary shaker operating at 135 r x min(-1), GA was converted into one major product and five minor products. The products were extracted and purified by solvent extraction, macroporous adsorbent resin, silica gel column chromatography, and semi-preparative RP-HPLC chromatography. Their structures were identified as 3-oxo-15α-hydroxy-18ß-glycyrrhetinic acid(1), 3-oxo-15ß-hydroxy-18ß-glycyrrhetinic acid (2), 7ß,15α-dihydroxy-18ß-glycyrrhetinic acid (3), 3-oxo-7ß, 15α-dihydroxy-18ß-glycyrrhetinic acid (4), 7ß-hydroxy-18ß-glycyrrhetinic acid(5) and 15α-hydroxy-18ß-glycyrrhetinic acid(6) by the analyses of MS, 1H-NMR and 13C-NMR spectroscopic data respectively. Among them, 2 was a new compound. These results suggest that C. blakesleeana CGMCC 3.970 has the capability of selective ketonization and hydroxylation for GA. [Key words] glycyrrhetinic acid; Cunninghamella blakesleeana CGMCC 3. 970; microbial transformation


Assuntos
Cunninghamella/metabolismo , Ácido Glicirretínico/análogos & derivados , Biotransformação , Ácido Glicirretínico/química , Ácido Glicirretínico/metabolismo , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray
20.
Yao Xue Xue Bao ; 49(9): 1279-88, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25518327

RESUMO

The tumor multidrug resistance reversal effect of NPB304, a novel taxane, was studied. MTT assay was used to determine the IC50 of chemotherapy drugs. Western blotting assay was applied to analyze the expression of P-glycoprotein (P-gp). The effect of compounds on the P-gp function and P-gp ATPase activity was determined by rhodamine 123 (Rh123) accumulation assay and analysis kit, respectively. Molecular docking was employed to predict the binding force between compounds and P-gp. Transmembrane transport of NPB304 was analyzed using MDCK II and MDR1-MDCK II cell model. NPB304 displayed multidrug resistance reversal effect on KBV cells and MCF-7/paclitaxel cells, NPB304 collaborative with P-glycoprotein (P-gp) inhibitors verapamil enhanced the reversal activity, specifically, 10 µmol x L(-1) verapamil in combination with paclitaxel reversed resistance by 56.5-fold, while combined with NPB304 increased the reversal fold; NPB304 synergistically increased Rh123 accumulation in the resistant cells when combined with verapamil, and NPB304 at 0-1 µmol x L(-1) enhanced the ATPase activity activated by verapamil was observed. NPB304 existed the hydrophobic interactions with the TM regions of P-gp, and the binding force between NPB304 and the A chain of the TM region was stronger. P-gp ATPase activity assay demonstrated NPB304 at lower concentrations (0-1.5 µmol x L(-1)) could activate the P-gp ATPase, playing a role on inhibition of P-gp function. However, NPB304 did not have an obvious feature of P-gp substrate. NPB304 exerted itself and synergy with verapamil activity on reversing tumor resistance via inhibiting the P-gp function.


Assuntos
Antineoplásicos/farmacologia , Taxoides/farmacologia , Verapamil/farmacologia , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Transporte Biológico , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Sinergismo Farmacológico , Humanos , Células MCF-7 , Rodamina 123
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