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1.
FASEB J ; 36 Suppl 12022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35556612

RESUMO

Ras proteins are among the most widely studied proto-oncogene products. Ras proteins are membrane-anchored GTPases that participate in multiple signaling cascades regulating crucial cellular processes including cell survival, proliferation, and differentiation. Ras mutations and/or deregulated Ras activities frequently lead to inflammation and malignant transformation. Recent studies have also shown that Ras mutations are associated with immuno-resistance by positive regulation of PD-L1 expression. Indoleamine 2,3-dioxygenases 1 (IDO1) is a heme-containing enzyme that catalyzes the rate limiting step of conversion of tryptophan into kynurenine (Kyn). Extensive research in the past has shown that IDO1 and its catalytic product Kyn mediate immune tolerance, thus promoting tumorigenesis in vivo. IDO1 expression is activated by immune cytokines including interferon-γ (IFN-γ)and interleukin-6 (IL-6). However, whether there is a mechanistic link between oncogenic KRas and IDO1 expression and how IDO1 expression contributes to immune invasion of transformed cells with KRas mutations remain unclear. Here we report that oncogenic KRas significantly enhanced IFN-γ-induced IDO1 expression. In H358 lung carcinoma cells, IDO1 expression induced by IFN-γ was at least in part dependent on ERK activation, and treatment with ARS-1620, a covalent KRasG12C inhibitor, suppressed IDO1 expression induced by IFN-Î³αµµα in a concentration-dependent manner. IDO1 expression was also induced by IFN-γ in HCT116 colon carcinoma cells that harbored a KRasG12D mutant allele, but not in HCT116 cells with wild-type KRas, suggesting that IFN-γ induced IDO1 expression requires Ras activation. In addition, KRas downregulation by specific siRNAs decreased IFN-γ-induced IDO1 expression in A549 lung cancer cells. Taken together, our study strongly suggests that Ras/ERK signaling pathway plays an important role in promoting IDO1 expression induced by IFN-γ.

2.
Molecules ; 27(9)2022 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-35566241

RESUMO

Six new diterpenoids, blusamiferoids A-F (1-6), including four pimarane-type diterpenoids, one rosane-type diterpenoid (3), and one rearranged abietane-type diterpenoid (6), were isolated from the dry aerial parts of Blumea balsamifera. Their structures were characterized by spectroscopic and computational methods. In particular, the structures of 1 and 4 were confirmed by X-ray crystallography. Compounds 5 and 6 were found to dose-dependently inhibit the production of TNF-α, IL-6, and nitrite oxide, and compound 5 also downregulated NF-κB phosphorylation in lipopolysaccharide (LPS)-induced RAW 264.7 cells.

3.
JMIR Serious Games ; 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35575761

RESUMO

BACKGROUND: Coronavirus Disease 2019 has spread worldwide and generated tremendous stress on human beings. Unfortunately, it is often hard for distressed individuals to access mental health services under conditions of restricted movement or even lockdown. In this research, we developed an online coloring game and tested its effectiveness as an accessible and engaging instrument for maintaining subjective well-being (SWB) as well as for reducing anxiety during the pandemic. OBJECTIVE: The study first aims to develop an online digital intervention package on a commercial released coloring game. The second aim is to test the effectiveness of difference interventions package for players to maintain SWB and reduce anxiety during the pandemic. METHODS: An evidence-based coloring intervention package was developed and uploaded to an online coloring game covering almost 1.5 million players worldwide in January 2021. Global players participated to color either four rounds of images characterized by awe, pink, nature, and blue or four rounds of irrelevant images. Participants' subjective well-being (SWB), anxiety, and perceived effectiveness of the game in reducing anxiety (subjective effectiveness, SE) were assessed one week before the intervention (T1), after completing pictures in each round (T2-T5), and after the intervention (T6). Independent t-tests were used to examine the general intervention effect and the intervention effect of each round. One-way ANOVA was used to examine whether these outcome variables were influenced by the number of rounds completed. RESULTS: In total, 1390 global players responded and completed at least one assessment. Overall, the general intervention group showed a statistical significantly greater increase in SWB than the general control group (N=164, t=3.59, Cohen's d 0.58[95% CI 0.36 to -1.24], P<0.001). Compared to the control group, the best effectiveness of intervention group showed in the Awe round, in which the increasing of SWB is significant (N=171, t=2.51, Cohen's d 0.39[95% CI 0.10 to -0.82], P=0.013), and players who colored all four pictures had nearly significant improvement in SWB (N=171, F(4,170)=2.34, ŋ2=0.053, P=0.058) and a significant decrease in anxiety(N=171, F(4,170)=3·39, ŋ2=0·075, P=0.011). CONCLUSIONS: These data suggest that the effectiveness of online psychological interventions, such as coloring game, for mental health in the special period. It shows the feasibility of applying existing commercial games embedded scientific psychological interventions that can fill the gap of mental crises and services for a wider group of people during the pandemic. The result would inspire the innovations of against the psychological problems caused by public emergencies, and encourages more games, especially most popular ones, to take more positive action for the common crisis of mankind.

4.
Artigo em Inglês | MEDLINE | ID: mdl-35507407

RESUMO

A Gram-stain-negative, non-motile, facultatively anaerobic and non-flagellated marine bacterium, designated JC070T was isolated from the Yongle Blue Hole in the South China Sea. The temperature, pH and NaCl ranges for growth of strain JC070T were 4-37 °C (optimum, 16 °C), pH 6.0-9.0 (optimum, pH 7.0) and 1.0 -6.0% (w/v; optimum, 3.0%). The predominant isoprenoid quinone of strain JC070T was identified as menaquinone-7. The dominant fatty acids (>10%) were iso-C15:0 (59.6%) and iso-C17:0 3-OH (17.2%). The major polar lipids were aminophospholipid, aminolipid, two unknown phospholipids and two unidentified lipids. The genomic DNA G+C content was determined to be 37.0 mol%. Based on the results of polyphasic analysis, a new species, named Marinifilum caeruleilacunae sp. nov., within the genus Marinifilum was proposed. The type strain is JC070T (= JCM 39045T=MCCC 1K03774T).


Assuntos
Flavobacteriaceae , Técnicas de Tipagem Bacteriana , Bacteroidetes , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Filogenia , RNA Ribossômico 16S/genética , Água do Mar/microbiologia , Análise de Sequência de DNA
6.
Cancers (Basel) ; 14(9)2022 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-35565251

RESUMO

This systematic review aims to identify prognostic molecular biomarkers which demonstrate strong evidence and a low risk of bias in predicting the survival of nasopharyngeal carcinoma (NPC) patients. The literature was searched for on PubMed to identify original clinical studies and meta-analyses which reported associations between molecular biomarkers and survival, including ≥150 patients with a survival analysis, and the results were validated in at least one independent cohort, while meta-analyses must include ≥1000 patients with a survival analysis. Seventeen studies fulfilled these criteria-two studies on single nucleotide polymorphisms (SNPs), three studies on methylation biomarkers, two studies on microRNA biomarkers, one study on mutational signature, six studies on gene expression panels, and three meta-analyses on gene expressions. The comparison between the hazard ratios of high-risk and low-risk patients along with a multivariate analysis are used to indicate that these biomarkers have significant independent prognostic values for survival. The biomarkers also indicate a response to certain treatments and whether they could be used as therapeutic targets. This review highlights that patients' genetics, epigenetics, and signatures of cancer and immune cells in the tumor microenvironment (TME) play a vital role in determining their survival.

7.
J Colloid Interface Sci ; 622: 539-548, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35526412

RESUMO

Enhancing the interfacial charge carriers transfer efficiency is important for designing photocatalysts with excellent hydrogen evolution performance. In this work, we have successfully constructed a In2O3@InN/ZnIn2S4 ternary heterostructure by embedding InN at the interface of thin-layered ZnIn2S4 and tubular In2O3 derived from metal-organic frameworks (MOFs) nanorods for the first time. The InN can not only adjust the energy band structure of In2O3, but also boost the photogenerated charge carriers transfer at the interface of In2O3 and ZnIn2S4. The optimum photocatalytic hydrogen evolution rate of In2O3@InN/ZnIn2S4 composite reaches 275 µmol/h (50 mg of catalyst) under simulated sunlight irradiation, which is obviously higher than pure In2O3 (12.5 times), ZnIn2S4 (2.5 times) and binary In2O3/ZnIn2S4 (1.8 times) photocatalysts. This work can offer a meaningful strategy to promote the interfacial charge separation in the heterostructure for excellent photocatalytic hydrogen evolution activity.

8.
Ann Surg Oncol ; 2022 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-35368219

RESUMO

PURPOSE: The aim of this study was to define a threshold of minimal clinically important improvement (MCII) for interpreting patient condition following video-assisted thoracoscopic surgery (VATS). METHODS: Patients undergoing VATS were recruited for this multicenter, prospective, observational cohort study. Symptoms were measured using the MD Anderson Symptom Inventory-Lung Cancer Module perioperatively. To define MCIIs, we first identified index symptoms, defined as the most severe symptoms showing the largest reduction from day 1 post-surgery to discharge. MCIIs for each index symptom were then obtained via an anchor-based approach. Symptom recovery was defined as an MCII after post-surgery day 1. Cox regression models were used to identify risk factors for unrecovered index symptoms. RESULTS: Using 366 patients, we identified pain and fatigue as index symptoms after VATS. MCII was defined as a 30% reduction in pain or fatigue. At discharge, 22.6% of patients had not recovered from pain and 22.4% had not recovered from fatigue. Cox models found that risk factors for unrecovered pain were Charlson Comorbidity Index score ≥1 (hazard ratio [HR] 1.36, 95% confidence interval [CI] 1.04-1.77; p = 0.02) and preoperative neoadjuvant therapy (HR 2.78, 95% CI 1.13-6.83; p = 0.02). Malignancy was a risk factor for unrecovered fatigue (HR 1.47, 95% CI 1.02-2.13; p = 0.04). CONCLUSION: Pain and fatigue can be used as index measures for symptom recovery in patients following VATS. A 30% MCII represented meaningful recovery after VATS and could identify patients who may need extensive care after discharge.

9.
Front Cardiovasc Med ; 9: 858066, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35433864

RESUMO

Purpose: This study sought to determine the associations of co-existing hypertension and hyperhomocysteinemia (H-Hcy) with carotid vulnerable plaque features and subsequent vascular events. Methods: Symptomatic patients with carotid atherosclerosis were enrolled and underwent carotid magnetic resonance (MR) vessel wall imaging. The patients were divided into the following groups: co-existing hypertension and H-Hcy group; isolated hypertension group; isolated H-Hcy group; and control group. The morphological and compositional characteristics of carotid plaques were assessed on MR images and compared among different groups. Univariate and multivariate cox regressions were used to calculate the hazard ratio (HR) and corresponding 95% confidence interval (CI) of co-existing hypertension and H-Hcy in predicting subsequent vascular events after at least 1-year followed-up. Results: In total, 217 patients (mean age, 59.4 ± 11.9 years; 154 males) were recruited. Patients in co-existing hypertension and H-Hcy group had a significantly higher prevalence of carotid lipid-rich necrotic core (LRNC) than isolated H-Hcy and control group (73.2 vs. 43.3 vs. 50%, p = 0.015). During the median follow-up time of 12.2 ± 4.3 months, 61 (39.8%) patients experienced vascular events. After adjusting for baseline confounding factors, co-existing hypertension and H-Hcy (HR, 1.82; 95% CI, 1.01-3.27; p = 0.044), presence of carotid LRNC (HR, 2.25; 95% CI, 1.09-4.65; p = 0.029), and combination of co-existing hypertension and H-Hcy and carotid LRNC (HR, 2.39; 95% CI, 1.26-4.43; p = 0.007) were significantly associated with subsequent vascular events. Conclusions: Co-existing hypertension and H-Hcy are associated with carotid vulnerable plaque features, such as LRNC. Combining co-existing hypertension and H-Hcy with carotid vulnerable plaque features has a stronger predictive value for subsequent vascular events than each measurement alone.

10.
Ann Transl Med ; 10(6): 338, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35434043

RESUMO

Background: Evidence on the effects of regular physical activity (PA) on asthmatic adults is rather weak and inconsistent since previous studies were conducted based on the limited studies, various populations groups and single outcome. A systematic review (SR) and meta-analysis have a high level of evidence to comprehensively evaluate the effects of PA for adults with asthma based on the available data. Our study aims to provide an SR of available evidence regarding the effect of regular PA on asthma in adults. Methods: A comprehensive search strategy was conducted using the PubMed, EMBASE, Cochrane Library, and Web of Science databases from their inception to October 2021. We identifying the eligible studies based on the PIOS principles, namely, populations (adults with asthma), interventions (regular PA), outcomes (quality of life or relapse-related outcomes), study design [randomized controlled trials (RCTs)]. A Bayesian-based meta-analysis was performed to pool available evidence. Quality assessment for individual studies was performed with the Cochrane risk of bias tool and the Newcastle-Ottawa Scale (NOS). Results: A total of 22 publications (18 RCTs and 4 longitudinal studies) were identified, comprised of 85,392 individuals aged between 18 and 75 years old. Overall quality of the included studies was rated as low-to-moderated quality. We found that PA was effective in improving quality of life (QOL) [health related quality of life, (HRQoL) & Asthma Control Questionnaire, (ACQ): standard mean difference (SMD) =-0.80, 95% credible interval (CrI): -1.30 to -0.31; I2=86.9%, Pheterogeneity<0.001], pulmonary function (FEV1/pred) [weighted mean difference (MD) =0.47, 95% CrI: 0.03 to 0.90; I2=74.9%, Pheterogeneity<0.001], and maximal oxygen consumption (VO2max) (MD =1.18, 95% CrI: 0.87 to 1.48; I2=17.0%, Pheterogeneity=0.31). Based on the longitudinal studies, the long-term high-level PA group had a lower risk of developing asthma compared with the low-level PA group [odds ratio (OR) =0.87, 95% CrI: 0.78 to 0.95; I2=27.4%, Pheterogeneity=0.22)]. Discussion: Adults with asthma need to carried out regular PA in accordance with the recommendations, which will improve their QOL and pulmonary function, and moreover, long-term PA appears to be more beneficial for asthmatic patients. The quality and quantity of included studies might affect the interpretation.

11.
Int J Pharm ; 619: 121716, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35367586

RESUMO

In the current times, achieving specific targeted and controllable drug delivery remains one of the major challenges in the treatment of hepatocellular carcinoma (HCC). The present study reported the development of a multiple functional indocyanine green (ICG)-cyclodextrin (CD) system, wherein loaded etoposide (EPS) was used as the model chemotherapeutic drug. In the developed system, ICG segment served as a photosensitizer for photothermal therapy (PTT) and the targeting moiety, which was primarily attributed to the specific retention properties in HCC tissues. The Ex vivo evaluation showed that ICG-CD@EPS exhibited a laser-triggered release profile with the photothermal efficiency and cytotoxicity towards HepG2 cells. In vivo evaluation suggested that ICG could navigate the systems to HCC tissues and retained at the site for 48 h, producing a drug accumulation in HCC. Further, laser irradiation boosted EPS release and local hyperthermia effects in HCC. Thus, the present study explored a novel and specific HCC targeting mechanism, and provided a feasible and controllable strategy for synergistic PTT and chemotherapy treatments for HCC.


Assuntos
Carcinoma Hepatocelular , Hipertermia Induzida , Neoplasias Hepáticas , Nanopartículas , Fotoquimioterapia , Carcinoma Hepatocelular/tratamento farmacológico , Linhagem Celular Tumoral , Humanos , Verde de Indocianina , Neoplasias Hepáticas/tratamento farmacológico , Fototerapia
13.
Phytochemistry ; 199: 113184, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35405148

RESUMO

Five undescribed benzopyran containing meroterpenoids, ganodercins Q-U, two undescribed benzofuran containing meroterpenoids, ganodercins V and W, and two known meroterpenoids were isolated from Ganoderma cochlear. Their structures were elucidated by using HRESIMS, NMR spectroscopy and computational methods. The results of biochemical studies using a palmitic acid (PA) induced insulin resistance (IR) model show that (-)-ganodercin Q, (+)-ganodercins R and W activate phospho-AKT (p-AKT) at 20 µM and improve glucose uptake in a concentration dependent manner. The results of renoprotection studies show that (+)-ganodercin S, cochlearol F, (+)- and (-)-ganodercins V reduce the expression of collagen I.

14.
Breast Cancer Res Treat ; 193(3): 659-667, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35429320

RESUMO

PURPOSE: Breast-conserving surgery (BCS) followed by whole breast radiation therapy (BCS-WBRT) or total mastectomy without WBRT (TM-no-WBRT) is the primary treatment for early stage breast cancer patients. Our study aimed to identify which early stage breast cancer treatment strategies had a subsequent lower incidence rate of mood disorder over a period of 10 years after the primary treatment. METHODS: This retrospective cohort study consisted of newly diagnosed early stage breast cancer patients in Taiwan from 2000 to 2013 using the National Health Insurance Research Database in Taiwan. We used a 1:1 propensity score matching by age to enrol patients into the BCS-WBRT and TM-no-WBRT groups. Statistical analyses were performed to calculate the hazard ratio and cumulative incidence rate. RESULTS: Our study consisted of 876 BCS-WBRT patients and 1949 TM-no-WBRT patients. After propensity score matching, each study group included 876 patients. The results showed that the mood disorder incidence rate was lower in the BCS-WBRT group than in the TM-no-WBRT group. Multivariate Cox regression analysis revealed that the BCS-WBRT group had a decreased risk of developing mood disorder (adjusted hazard ratio 0.69, 95% CI 0.53-0.90, p < 0.01). Furthermore, the Kaplan-Meier analysis showed that the BCS-WBRT group had a lower cumulative incidence rate of mood disorder, especially depression, after undergoing 10 years of primary treatment (p = 0.004). CONCLUSION: Our results indicated that BCS-WBRT was associated with a lower risk of development of mood disorder over a 10-year period compared to TM-no-WBRT in early stage breast cancer patients. Our findings may provide helpful information, along with other clinical data, for breast cancer patients as they choose the type of appropriate surgery for treatment.

15.
Molecules ; 27(7)2022 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-35408527

RESUMO

Metal-organic framework (MOF) films are essential for numerous sensor and device applications. However, metal-organic framework materials have poor machinability due to their predominant powder-like nature, and their presence as the active layer in a device can seriously affect the performance and utility of the device. Herein, active layers of field-effect transistor (FETs) devices and chemiresistor gas sensors with high performance were constructed by loading Cu3(HITP)2 (HITP = 2,3,6,7,10,11-hexaiminotriphenylene) in situ-axial anchoring on oriented nanofiber arrays prepared via electrospinning. The strong interaction between polar groups on the polymer chains and metal ions promotes the nucleation of Cu3(HITP)2, steric hindrance makes particles of Cu3(HITP)2 with uniform size, morphology, and good crystallinity during nucleation by liquid phase epitaxial growth (LPE). Influences of differently-oriented Cu3(HITP)2 NFAs-based FETs on the electrical properties were studied, optimally oriented Cu3(HITP)2 NFAs-based FETs showed good mobility of 5.09 cm2/V·s and on/off ratio of 9.6 × 103. Moreover, excellent gas sensing response characteristics were exhibited in sensing volatile organic compounds (VOCs). Chemiresistor gas sensors with high response value, faster response and recovery are widely suited for VOCs. It brings new inspirations for the design and utilization of electrically conductive MOFs as an active layer for FETs and sensor units for chemiresistor gas sensors.

16.
Med Biol Eng Comput ; 60(6): 1613-1626, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35397109

RESUMO

Gas embolism is a potentially serious complication of hysteroscopic surgery. It is particularly necessary to monitor bubble parameters in hysteroscopic images by computer vision method for helping develop automatic bubble removal devices. In this work, a framework combining a deep edge-aware network and marker-controlled watershed algorithm is presented to extract bubble parameters from hysteroscopy images. The proposed edge-aware network consists of an encoder-decoder architecture for bubble segmentation and a contour branch which is supervised by edge losses. The post-processing method based on marker-controlled watershed algorithm is used to further separate bubble instances and calculate size distribution. Extensive experiments substantiate that the proposed model achieves better performance than some typical segmentation methods. Accuracy, sensitivity, precision, Dice score, and mean intersection over union (mean IoU) obtained for the proposed edge-aware network are observed as 0.859 ± 0.017, 0.868 ± 0.019, 0.955 ± 0.005, 0.862 ± 0.005, and 0.758 ± 0.007, respectively. This work provides a valuable reference for automatic bubble removal devices in hysteroscopic surgery.


Assuntos
Aprendizado Profundo , Redes Neurais de Computação , Algoritmos , Processamento de Imagem Assistida por Computador/métodos
17.
Clin Chim Acta ; 531: 62-67, 2022 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-35292254

RESUMO

BACKGROUND: Visinin-like protein 1 (VILIP-1) appears as a biomarker of neuronal injury. We investigated the correlation of serum VILIP-1 concentrations with severity, early neurologic deterioration (END) and functional outcome of intracerebral hemorrhage (ICH). METHODS: In this prospective and observational study, serum VILIP-1 concentrations were quantified in 106 patients with basal ganglia hemorrhage. Univariate and multivariable logistic regression analyses were used to analyze the relationship between serum VILIP-1 concentrations and END plus worse prognosis (modified Rankin Scale score of 3 or greater) at post-injury 3 months. RESULTS: Serum VILIP-1 concentrations of patients were closely correlated with hematoma volume and National Institutes of Health Stroke Scale score. Serum VILIP-1 concentrations were substantially elevated in patients with END or worse 3-month prognosis, as compared to other remainders. Also, serum VILIP-1 concentrations were independently associated with END and worse 3-month prognosis. Under ROC curve analysis, serum VILIP-1 concentrations exhibited marked accuracy for distinguishing patients with the development of END or worse 3-month prognosis. Its predictive ability was in the range of hematoma volume and National Institutes of Health Stroke Scale score. CONCLUSIONS: Serum VILIP-1 may be a good biomarker for assessing hemorrhagic severity and clinical outcomes after ICH.

18.
Curr Oncol ; 29(3): 2046-2063, 2022 03 17.
Artigo em Inglês | MEDLINE | ID: mdl-35323365

RESUMO

Canadian provinces routinely collect patient-level data for administrative purposes. These real-world data (RWD) can be used to generate real-world evidence (RWE) to inform clinical care and healthcare policy. The CanREValue Collaboration is developing a framework for the use of RWE in cancer drug funding decisions. A Data Working Group (WG) was established to identify data assets across Canada for generating RWE of oncology drugs. The mapping exercise was conducted using an iterative scan with informant surveys and teleconference. Data experts from ten provinces convened for a total of three teleconferences and two in-person meetings from March 2018 to September 2019. Following each meeting, surveys were developed and shared with the data experts which focused on identifying databases and data elements, as well as a feasibility assessment of conducting RWE studies using existing data elements and resources. Survey responses were compiled into an interim data report, which was used for public stakeholder consultation. The feedback from the public consultation was used to update the interim data report. We found that databases required to conduct real-world studies are often held by multiple different data custodians. Ninety-seven databases were identified across Canada. Provinces held on average 9 distinct databases (range: 8-11). An Essential RWD Table was compiled that contains data elements that are necessary, at a minimal, to conduct an RWE study. An Expanded RWD Table that contains a more comprehensive list of potentially relevant data elements was also compiled and the availabilities of these data elements were mapped. While most provinces have data on patient demographics (e.g., age, sex) and cancer-related variables (e.g., morphology, topography), the availability and linkability of data on cancer treatment, clinical characteristics (e.g., morphology and topography), and drug costs vary among provinces. Based on current resources, data availability, and access processes, data experts in most provinces noted that more than 12 months would be required to complete an RWE study. The CanREValue Collaboration's Data WG identified key data holdings, access considerations, as well as gaps in oncology treatment-specific data. This data catalogue can be used to facilitate future oncology-specific RWE analyses across Canada.


Assuntos
Antineoplásicos , Neoplasias , Antineoplásicos/uso terapêutico , Canadá , Humanos , Neoplasias/tratamento farmacológico
19.
Heart Vessels ; 37(6): 1085-1096, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35320391

RESUMO

Atherosclerosis (AS) is the basic lesion underlying the occurrence and development of cerebrovascular diseases. Abnormal proliferation of vascular smooth muscle cells (VSMCs) plays a crucial role in AS. We aimed to explore the role of SNHG16 in AS and the molecular mechanism of VSMC involvement in the regulation of AS. The expression levels of SNHG16, miR-30c-5p and SDC2 were detected by qRT-PCR. CCK-8, wound healing and Transwell assays were used to assess ox-LDL-induced VSMC proliferation, migration, and invasion, respectively. Western blot analysis was used to detect SDC2 and MEK/ERK pathway-related protein levels. A dual-luciferase reporter assay confirmed the binding of SNHG16 with miR-30c-5p and miR-30c-5p with SDC2. SNHG16 and SDC2 expression was upregulated in patients with AS and ox-LDL-induced VSMCs, while miR-30c-5p was downregulated. Ox-LDL-induced VSMC proliferation and migration were increased, and the MEK/ERK signalling pathway was activated. MiR-30c-5p was targeted to SNHG16 and SDC2. Downregulating SNHG16 or upregulating miR-30c-5p inhibited ox-LDL-induced VSMC proliferation and migration and inhibited MEK/ERK signalling pathway activation. In contrast, downregulating miR-30c-5p or upregulating SDC2 reversed the effects of downregulating SNHG16 or upregulating miR-30c-5p. Furthermore, downregulating SDC2 inhibited ox-LDL-induced proliferation and migration of VSMCs and inhibited activation of the MEK/ERK signalling pathway, while upregulating lncRNA SNHG16 reversed the effects of downregulating SDC2. Downregulation of SNHG16 inhibited VSMC proliferation and migration in AS by targeting the miR-30c-5p/SDC2 axis. This study provides a possible therapeutic approach to AS.

20.
J Pharm Biomed Anal ; 214: 114722, 2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35325799

RESUMO

Pharmaceutical drugs like Sildenafil are illegally added to functional food such as nutritional supplements and herbal remedies to deliver drugs without a regular prescription to consumers. Rapid screening of illegal additives is desirable for the public security department. The seized samples are often large in number and unknown in composition; methods are needed for qualitative screening of unknown samples. Here, a new approach is presented based on atmospheric pressure solids analysis probe (ASAP) coupled with single-quadrupole mass spectrometer to rapidly screen 42 common illegal additives in six categories of functional food. The ASAP-MS method could be applied to solid or liquid sample analysis with a very simple pre-treatment and no LC chromatographic separation, using a home-built library; the identification of suspicious additives could be obtained rapidly. More importantly, the approach is sensitive enough for complex matrix samples like coffee samples. 21 batches of seized unknown samples were tested by the ASAP-MS, and the positive results were confirmed by LC-MS/MS(QQQ), indicating that the ASAP-MS method is effective and reliable. The ASAP-MS with home-built library is a promising method for rapid screening of illegal additives in functional food, which could be widely used in the grassroots police station that lack professional laboratory environment.


Assuntos
Alimento Funcional , Espectrometria de Massas em Tandem , Pressão Atmosférica , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida
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