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1.
Chin Med ; 16(1): 70, 2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34348770

RESUMO

It is well-known that Prof. Tu Youyou won the Nobel Prize in Physiology or Medicine in 2015 due to the research on artemisinin treating malaria, and this can be regarded as the milestone of modernization of Traditional medicine. This first Nobel Prize in Traditional Chinese medicine (TCM) has aroused profound impetus in the investigation of TCM and attracted global attention to the ancient books of TCM. Three new medicines for the treatment of COVID-19 derived from Chinese Classical Formula (, CCF) have been approved in 2021 due to their effectiveness for the treatment of COVID-19. This article introduced the research background of CCF pharmaceutical preparation (CCFPP), explained the ideas for the modernization of CCF and analyzed related issues involved in the development process of CCFPP, including the origin of medicinal materials, processing methods, dosages and the preparation process of CCF Material Reference. The strategy for industrialization was proposed in terms of the evaluation of the pharmaceutical properties, industrialization considerations, and clinical positioning of CCFPP. The key contents and requirements for the development CCFPP were also summarized according to the recently published registration guidance by the Center for Drug Evaluation in China. In addition, the safety issues of CCFPP were described, including the discussion on the non-clinical safety evaluation and analyzation on the international registration of Traditional herbal medicines. This article is aimed to provide references for enterprises, researchers, and relevant personnel of government departments that are engaged in the development of CCF to speed up the developing process of CCFPP.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34306157

RESUMO

Silkworm droppings are the product of mulberry leaves digested by silkworm intestines, which are an important medicinal resource in traditional Chinese medicine (TCM). The contents of total fat, fat acids, crude protein, amino acids, and secondary metabolites of obtained mulberry leaves and silkworm droppings were analyzed by HPLC, GC-MS, and UHPLC-Q-TOF MS. The target genes and enriched pathways related to significantly changed compositions between mulberry leaves and silkworm droppings were analyzed by network pharmacology. High unsaturated C18 : 3 fatty acids were transformed to low unsaturated C18 : 1 from mulberry leaves to silkworm droppings. Only lysine and 17 mini-peptides had significantly higher content in silkworm droppings than in mulberry leaves. There were 36 common target genes or the different compounds between mulberry leaves and silkworm droppings. The main pathways of mulberry leaf were enriched in antivirus and anticancer properties, while the pathways of silkworm droppings were enriched in hormone regulation and signal transduction.

3.
Chin Med ; 16(1): 50, 2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34215302

RESUMO

BACKGROUND: It is essential to identify the chemical components for the quality control methods establishment of Chinese Classical Formula (CCF). However, CCF are complex mixture of several herbal medicines with huge number of different compounds and they are not equal to the combination of chemical components from each herb due to particular formula ratio and preparation techniques. Therefore, it is time-consuming to identify compounds in a CCF by analyzing the LC-MS/MS data one by one, especially for unknown components. METHODS: An ultra-high pressure liquid chromatography-linear ion trap-orbitrap high resolution mass spectrometry (UHPLC-LTQ-Orbitrap-MS/MS) approach was developed to comprehensively profile and characterize multi-components in CCF with Erdong decoction composed of eight herbal medicines as an example. Then the MS data of Erdong decoction was analyzed by MS/MS-based molecular networking and these compounds with similar structures were connected to each other into a cluster in the network map. Then the unknown compounds connected to known compounds in a cluster of the network map were identified due to their similar structures. RESULTS: Based on the clusters of the molecular networking, 113 compounds were rapidly tentative identification from Erdong decoction for the first time in the negative mode, which including steroidal saponins, triterpenoid saponins, flavonoid O-glycosides and flavonoid C-glycosides. In addition, 10 alkaloids were tentatively identified in the positive mode from Nelumbinis folium by comparison with literatures. CONCLUSION: MS/MS-based molecular networking technique is very useful for the rapid identification of components in CCF. In Erdong decoction, this method was very suitable for the identification of major steroidal saponins, triterpenoid saponins, and flavonoid C-glycosides.

4.
Comput Math Methods Med ; 2021: 6691356, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34122619

RESUMO

Chronic spontaneous urticaria (CSU) is a common skin disease which symptom is local pruritus and pain. In medicine, researchers take a certain point that the brain is the control center of CSU, but in previous experiments, the researchers found that cerebellum also had a certain effect on CSU. In order to find out the influence of CSU in the brain and cerebellum, we collected the brain resting-state fMRI data from 40 healthy controls and 32 CSU patients and used DPABI to preprocess. We calculated the entropy values of five scales by using multiscale entropy (MSE) and the average entropy values of two groups' BOLD signals; 15 regions with significant differences were found which not only had a more detailed impact in the brain but also had an impact in the cerebellum, such as precentral gyrus, lenticular putamen, and vermis of cerebellum. In addition, we found that compared with the healthy controls, the entropy values of CSU patients showed two trends which need further study. The advantage of our experiment is that the multiscale entropy value is used to get more influence regions of CSU in the brain and cerebellum. The results of this paper may provide some help for the pathological study of CSU.


Assuntos
Urticária Crônica/diagnóstico por imagem , Adulto , Idoso , Algoritmos , Encéfalo/diagnóstico por imagem , Estudos de Casos e Controles , Cerebelo/diagnóstico por imagem , Urticária Crônica/sangue , Urticária Crônica/patologia , Biologia Computacional , Entropia , Feminino , Neuroimagem Funcional , Humanos , Interpretação de Imagem Assistida por Computador/métodos , Interpretação de Imagem Assistida por Computador/estatística & dados numéricos , Modelos Lineares , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Oxigênio/sangue
5.
Chemosphere ; 262: 127477, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32799136

RESUMO

Focus on the safety of herbal medicines has mainly been directed towards the presence of intrinsic toxicity, as found in the cases of renal and hepatic dysfunction caused by aristolochic acids. However, contamination from extrinsic hazards may impart an even greater reduction in their safety and efficacy. This study reveals that pesticides were present in the majority (88%) of a comprehensive cross-section (n = 1771) of herbal medicine samples. Alarmingly, more than half (59%) contained pesticides over the European Pharmacopoeia (EP) limit, and 43% of them contained 35 varieties of banned, extremely toxic pesticides, eight of which were detected at levels over 500 times higher than the default Maximum Residue Limit (MRL). DDTs, carbofuran, and mevinphos were confirmed as being among the most risk-inducing pesticides by three different risk assessment methods, reported to produce carcinogenic, genotoxic, reproductive, and developmental effects, in addition to carrying nephrotoxicity and hepatotoxicity. In light of these findings, and withstanding that extrinsic hazards can be controlled unlike intrinsic toxicity, the authors here strongly recommend the application of herbal medicine quality-control measures and solutions to safeguard against a neglected but certainly potentially serious health risk posed to the majority of the global population that consumes herbal medicines.


Assuntos
Contaminação de Medicamentos , Medicamentos de Ervas Chinesas/análise , Praguicidas/análise , Carbofurano , Cromatografia Gasosa , Cromatografia Líquida/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Resíduos de Praguicidas/análise , Medição de Risco , Espectrometria de Massas em Tandem/métodos
6.
J Transl Med ; 18(1): 372, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-32993688

RESUMO

An amendment to this paper has been published and can be accessed via the original article.

7.
Zhongguo Zhong Yao Za Zhi ; 45(1): 119-123, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-32237420

RESUMO

Astragali Radix is commonly used as bulk medicinal materials. Chinese Pharmacopoeia contains about 150 compound preparations of Astragali Radix, but the sample preparation method under the determination of Astragali Radix content in Chinese Pharmacopoeia is tedious and time-consuming, not convenient for the test of a large number of samples. Therefore, it is of great significance to simplify the sample preparation method and improve the practicability of the method for the quality control of Astragali Radix and its preparations. In this study, ultrasonic extraction method was used instead of heated reflux extraction, and solid phase extraction method was used to enrich and prepare the samples. A set of practical quality evaluation method was established for Astragali Radix slices and standard decoction, greatly shortening the sample preparation time and improving the accuracy of the method. The results of Astragali Radix standard decoction analysis showed that the transfer rate of calycosin 7-O-ß-D-glucospyranoside,(96.5±28.7)%, had great variation, which was found to be related to the conversion of mulberry isoflavone glucoside into calycosin 7-O-ß-D-glucospyranoside during the preparation of standard decoction. The transfer rates were(59.4±14.4)% and(101.3±12.3)% for calycosin and astragaloside Ⅳ respectively, which were relatively stable. Therefore, it is suggested that Astragali Radix slices and water decoction preparations should be evaluated by using calycosin and astragaloside Ⅳ as the quality evaluation index. The results provide a scientific and practical method for quality control of Astragali Radix slices and its standard decoction, and also provide scientific evidence for quality evaluation of the preparations.


Assuntos
Astrágalo (Planta)/química , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/normas , Glucosídeos/análise , Raízes de Plantas/química , Controle de Qualidade , Extração em Fase Sólida
8.
Zhongguo Zhong Yao Za Zhi ; 45(4): 884-889, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237489

RESUMO

To establish a content determination method for quality control of the pieces and standard decoction of honey-fried Descurainiae Semen. Standard decoction of honey-fried Descurainiae Semen was prepared with standardized process, and high performance liquid chromatography coupled with diode-array detector(HPLC-DAD) was used to detect its characteristic fingerprint and determine the content of quercetin-3-O-ß-D-glucose-7-O-ß-D-gentiobioside. In addition, the transfer rate, dry extract rate and pH value were calculated. The results showed that the established method had a high accuracy. The content of quercetin-3-O-ß-D-glucose-7-O-ß-D-gentiobioside in 13 batches of standard decoction was 0.03-0.12 mg·mL~(-1); the transfer rate was 13.4%-23.1%; the rate of extracts was 1.9%-5.5%, and the pH was between 5.4-5.9. The similarity coefficients were all greater than 0.85, indicating good homogeneity for the different batches of decoction. There were 7 common peaks in the characteristic chromatogram, one of which was quercetin-3-O-ß-D-glucose-7-O-ß-D-gentiobioside. In this paper, the established content determination and quality evaluation method for Descurainiae Semen pieces and decoction was simple, rapid and reproducible, providing reference for the quality control of honey-fried Descurainiae Semen pieces, standard decoction and its preparations.


Assuntos
Brassicaceae/química , Medicamentos de Ervas Chinesas/normas , Mel , Sementes/química , Cromatografia Líquida de Alta Pressão , Glucosídeos/análise , Controle de Qualidade , Quercetina/análogos & derivados
9.
Zhongguo Zhong Yao Za Zhi ; 45(6): 1363-1367, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32281349

RESUMO

The quality evaluation method for standard decoction of Chinese herbal slices is the basis for the quality evaluation of granules and preparations of classical formula(decoction)of traditional Chinese medicine. This study aimed to establish a method for the determination of quercetin-3-O-glucuronic acid in Nelumbinis Folium(NF)and its standard decoction, so as to provide reference for the quality control of NF and its standard decoction. Fifteen batches of representative NF were collected to prepare standard decoction, and the parameters of dry extract rate, transfer rate of index component, and pH value were calculated. HPLC was used to establish the content determination method for quercetin-3-O-glucuronic acid in NF and its standard decoction. The concentration range of quercetin-3-O-glucuronic acid in the standard decoction of NF was 1.09-3.06 g·L~(-1), while the concentration range of nuciferine was 0.01-0.17 g·L~(-1). The average extraction rate of NF standard decoction was(14.4±2.6)%, the average transfer rate of quercetin-3-O-glucuronic acid was(70.7±18.6)%, and the average transfer rate of nuciferine was(9.6±5.4)%. Compared with Nuciferine, quercetin-3-O-glucuronic acid had a high content and stable transfer rate in standard decoction, and was recommended to be the quality control marker for NF and its standard decoction. This paper establishes a quality evaluation method for NF standard decoction, and can provide reference for the quality control of all preparations derived from NF and its decoction.


Assuntos
Medicamentos de Ervas Chinesas/química , Flores/química , Nelumbo/química , Controle de Qualidade , Cromatografia Líquida de Alta Pressão , Medicina Tradicional Chinesa
10.
J Ethnopharmacol ; 250: 112460, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31837415

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Honey, an important additive with natural deep eutectic solvent (NADES) characteristics, has been used in traditional medicine for thousands of years. AIM OF THE STUDY: We investigated the quality-improving effects of honey on Astragali Radix (Mikvetch Root) (RA) as an example. MATERIALS AND METHODS: Decoctions of raw RA, fried RA, honey-fried RA, and a man-made- honey-fried RA were prepared and compared in cell-based bioactivity tests, chemical composition tests, as well as a bioavailability test with calycosin-7-O-ß-D-glucoside. RESULTS: The addition of honey increased the concentrations of active compounds and their oral bioavailability, provided protection against acetylation, and consequently increased their bioactivity. These changes were also observed when a pure NADES-mimicking honey was used. CONCLUSION: Our findings provide a potential explanation as to why honey has long been used as traditional medicine additives and rationalize the application of honey and honey-like substance in producing pharmaceuticals.


Assuntos
Astrágalo (Planta) , Mel , Extratos Vegetais/farmacologia , Raízes de Plantas , Acetilação , Animais , Disponibilidade Biológica , Feminino , Células HEK293 , Temperatura Alta , Humanos , Masculino , Medicina Tradicional , Metabolômica , Camundongos Endogâmicos ICR , Extratos Vegetais/farmacocinética , Ratos Sprague-Dawley , Solventes
11.
Front Pharmacol ; 11: 554777, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33390938

RESUMO

Due to the complex nature of traditional medicines, quality control methods need to cover two aspects: compliance of raw materials with quality standards and process control. Astragali radix (AR), the roots of Astragalus mongholicus Bunge, was selected in this study as an example of a widely used traditional medicine in various formulations. Astragaloside IV (AG IV) and calycosin 7-O-ß-D-glucoside (CG) are used as the markers for the quality control of AR and its products in the Chinese Pharmacopoeia. However, in the raw materials, malic acid esters of the CG and acetate esters of the astragaloside are easily decomposed into CG and AG IV during storage and processing of AR to make extracts for various preparations. The thermal stability of the isoflavonoids and astragalosides in decoction was studied. The level of CG and astragalosides (AG I/AG II/AG IV) was strongly affected by prolonged heat during processing, while calycosin was stable in the conditions. Also the major astragalosides in AR could fully converted into AG IV which eventually reaches a stable level under certain conditions. With calycosin and AG IV as marker components, practical, reproducible, and precise methods were established and applied to the quality analysis of AR from its raw materials to its intermediates and products. This study demonstrates that a full chemical profiles analysis of the whole manufacturing process (from "raw materials-intermediates/extracts-final product") is important to identify quality markers (Q-markers) and even to establish proper analysis methods for traditional Chinese medicine products.

12.
Zhongguo Zhong Yao Za Zhi ; 44(5): 865-869, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30989841

RESUMO

The health food industry is an important support for the big health industry and the strategy of healthy China. The Chinese medicine prescription health food has exceeded 60% of the total declared health food. However,the main basis for its function evaluation,the Technical Specification for Inspection and Evaluation of Health Food,was abolished in 2018,and 27 of them were based on modern medical and nutritional theories. Quantitative efficacy evaluation methods in western pharmacology are short of function claims and function evaluation methods reflecting the characteristics of traditional Chinese medicine,which could affect the health food industry to a certain extent. Therefore,the establishment of the evaluation mechanism of Chinese medicine prescription health food which conforms to the positioning of health food and the theory of traditional Chinese medicine is helpful for the healthy development of health food industry. In this paper,this problem was explained from five aspects. First,how to differentiate the positioning of Chinese medicine prescription health food from ordinary food and medicine,and how to embody the characteristics of Chinese medicine. Secondly,the relationship between traditional Chinese medicine prescription health food and Chinese patent medicine. Thirdly,how to scientifically and reasonably determine the raw materials of traditional Chinese medicine prescription health food. Fourthly,how to explain the function claim of traditional Chinese medicine prescription health food,and how to evaluate its function scientifically and reasonably. Fifthly,the functional evaluation of Chinese herbal medicine prescription health food is connected with other national scientific and technological strategies. In this paper,a preliminary analysis of the Chinese medicine prescription health food was conducted from the above five aspects,and some personal views and suggestions were put forward,hoping to provide reference for the competent authorities and researchers.


Assuntos
Indústria Alimentícia , Alimentos , Medicina Tradicional Chinesa , China
13.
Zhongguo Zhong Yao Za Zhi ; 44(5): 880-884, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-30989844

RESUMO

Health food containing Chinese materia medica has many advantages in health preservation and reducing the risk of disease occurrence,which meets people's demands for " great health" and " preventive treatment of disease". However,due to its complex ingredients,diverse quality of raw materials,as well as the vagueness and lack of integrity for existing quality standards,chaos is caused in the health food market,which restricts its healthy development and also poses new challenges to the quality control of healthy food. At present,the total component content or single component content is determined in most functional/marker component examinations. Safety and microbial detection methods fail to cover the contamination range of the raw materials of Chinese materia medica.Therefore,it is impossible to meet the purpose of ensuring authenticity,safety and efficacy. In recent years,a lot of Chinese materia medica extracts have been used as raw materials for food products,but many extracts lack standards. The author believes that the quality control of health food containing Chinese materia medicas should start with the quality control of Chinese materia medica extracts. In this way,product quality is controlled from source to ensure product consistency; secondly,the overall quality control should be strengthened to ensure the authenticity of the products; the scope of safety inspection shall be expanded to fundamentally ensure the safety of products. At the same time,we should strengthen the quality control of whole process and strengthen the overall quality control of raw materials to produce health food of high quality.


Assuntos
Alimentos/normas , Materia Medica/normas , Medicina Tradicional Chinesa , Controle de Qualidade , Projetos de Pesquisa
14.
J Ethnopharmacol ; 237: 47-54, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-30898554

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: The quality control of Traditional Chinese medicine (TCM) is a scientific problem and an industrial issue, which hampers the development of evidence based TCM. The concept of quality markers (Q-markers) is proposed and has been applied to the quality evaluation of TCM based on its clinical efficacy. However, more specific methods are needed to put this idea into practice. The standard decoction is a representative of decoction used in clinical practice and it can be used for the discovery of Q-markers related to the clinical efficacy of TCM. AIM OF THE STUDY: In this study, a systemic strategy was established to discover Q-markers related to the clinical efficacy of TCM Ephedrae Herba (EH), dried stem of Ephedra sinica Stapf. The different processed materials of EH have different clinical applications, though originating from the same medicinal herb. MATERIALS AND METHODS: The standard decoction of each of the processed materials was prepared and a 1HNMR metabolomics approach and total polysaccharide analysis were used to identify potential Q-markers related to the different clinical applications of EH. Correlation analysis was made of the measured biological activity and the holistic chemical profile. RESULTS: The results showed that total polysaccharides and alkaloids were Q-markers for EH preparations. CONCLUSION: This study demonstrates that the standard decoction is a reasonable research objective to explore chemical markers that correlate with the clinical efficacy of TCM.


Assuntos
Ephedra , Extratos Vegetais/farmacologia , Caules de Planta , Controle de Qualidade , Células HEK293 , Humanos , Luciferases/fisiologia , Metabolômica , Regiões Promotoras Genéticas , Receptor 2 Toll-Like/fisiologia , Fator de Transcrição AP-1/fisiologia , Resultado do Tratamento
15.
Zhongguo Zhong Yao Za Zhi ; 43(5): 861-867, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676079

RESUMO

Decoction of single medicinal herb is a reference for the standardization of different dosage forms of Chinese medicine and it provides a new direction for solving the problems existing in the quality of Chinese medicinal granules such as no uniform dosage form and no clear quality standard. In this paper, the quality evaluation method of standard decoction of rhubarb was established to provide reference for the quality control of common dosage forms such as clinical decoction and formula granule. 10 batches of representative Rhei Radix et Rhizoma were collected to establish UPLC fingerprints were established. The chemical structures of main peaks were identified with ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and the main components in the decoction were Anthraquinones. The extraction ratio of the standard decoction was (28.1±3.8)% and the transfer rate was (19.9±6.3)%. The method for the quality evaluation of standard decoction of Rhei Radix et Rhizoma was established in this study, providing reference for the quality control method of terminal products from decoction of Rhei Radix et Rhizoma.


Assuntos
Medicamentos de Ervas Chinesas/normas , Controle de Qualidade , Rheum/química , Antraquinonas/química , Cromatografia Líquida de Alta Pressão , Rizoma/química
16.
Zhongguo Zhong Yao Za Zhi ; 43(5): 868-872, 2018 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29676080

RESUMO

To establish the quality control methods for the standard decoction of Forsythiae Fructus. Twelve batches of representative Forsythiae Fructus were collected to prepare standard decoction of Forsythiae Fructus, and then the parameters such as extraction ratio, transfer rate of the index components and pH value of the solution were calculated to evaluate the stability of the process. The simultaneous determination method of target components and fingerprint method were established, and ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used to identify the main common peaks in the fingerprint to clarify the main chemical constituents in the decoction. The similarities of the fingerprints of standard decoction of Forsythiae Fructus were more than 0.9. The average extraction ratio of the standard decoction of Forsythiae Fructus was (15.53±6.27)%, and the transfer rate of forsythiaside A was(38.0±10.2)%. The method for evaluating the quality of standard decoction of Forsythiae Fructus was presented, providing reference for the quality control of products stemmed from the water extract of Forsythiae Fructus, with high similarity and uniform quality.


Assuntos
Medicamentos de Ervas Chinesas/normas , Forsythia/química , Extratos Vegetais/normas , Controle de Qualidade , Cromatografia Líquida de Alta Pressão , Frutas/química , Espectrometria de Massas , Plantas Medicinais/química
17.
Front Pharmacol ; 9: 1377, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30618731

RESUMO

Saponins are a class of important active ingredients. Analysis of saponin-containing herbal medicines is a major challenge for the quality control of medicinal herbs in companies. Taking the medicine Astragali radix (AR) as an example, it has been shown that the existing evaporative light scattering detection (ELSD) methods of astragaloside IV (AG IV) has the disadvantages of time-consuming sample preparation and low sensitivity. The universality of ELSD results in an inapplicable fingerprint with huge signals from primary compounds and smaller signals from saponins. The purpose of this study was to provide a practical and comprehensive method for the quality control of the astragalosides in AR. A simple sample preparation method with sonication extraction and ammonia hydrolyzation was established, which shortens the preparation time from around 2 days to less than 2 h. A UPLC-QDA method with the SIM mode was established for the quantification of AG IV in AR. Methanol extract was subjected to UPLC-QDA for fingerprinting analysis, and the common peaks were assigned simultaneously with the QDA. The results showed that with the newly established method, the preparation time for a set of samples was less than 90 min. The fingerprints can simultaneously detect both saponins and flavonoids in AR. This simple, rapid, and comprehensive UPLC-QDA method is suitable for quality assessment of RA and its products in companies, and also provides references for the quality control of other saponin ingredients without UV absorption.

18.
J Asian Nat Prod Res ; 20(9): 867-874, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29110534

RESUMO

Two new flavonol glycosides, limocitrin 3-O-ß-D-xylopyranosyl(1→2)-ß-D-glucopyranoside (1) and limocitrin 3-O[2-O-ß-D-xylopyranosyl-6-O-α-L-rhamnopyranosyl]-ß-D-glucopyranoside (2), together with eight known analogs (3-10), were isolated from the fruits of Evodia rutaecarpa. Their structures were elucidated on the basis of spectroscopic analyses and chemical evidences. Meanwhile, Nrf2 inducing abilities of seven isolated compounds were evaluated, and compounds 1, 2, 6, 7, and 8 exhibited moderate effect on Nrf2.


Assuntos
Evodia/química , Flavonóis/farmacologia , Frutas/química , Glicosídeos/química , Flavonóis/química , Regulação da Expressão Gênica/efeitos dos fármacos , Glicosídeos/farmacologia , Células HEK293 , Humanos , Estrutura Molecular , Fator 2 Relacionado a NF-E2/genética , Fator 2 Relacionado a NF-E2/metabolismo
19.
Zhongguo Zhong Yao Za Zhi ; 42(5): 809-816, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994518

RESUMO

Decoction of single medicinal herb is a reference for the standardization of different dosage form of Chinese medicine and it provides a new direction for solving the problems existing in the quality of Chinese medicinal granules such no uniform dosage forms and no clear quality standard. There are few reports on the idea, method and preparation of single herb standard decoction. Our country is in urgent need of that information in order to improve the consistency and stability of traditional Chinese medicine products. Here, Lonicerae Japinicae Flos was selected as an example to elucidate the preparation and quality evaluation of Chinese single herbal medicine decoction. Twelve batches of representative Lonicerae Japinicae Flos were collected, UPLC fingerprints were established, and the chemical structures of main peaks were identified with UPLC-QTOF-MS and standard compounds. The main components in the decoction are organic acids and iridoids. The extract rate of the standard decoction was (34.2±2.9)% and the transfer rate is (78.6±8.4)% in the form of chlorogenic acid, within the range of 75%-125% of mean. This paper established a method for the quality evaluation of standard decoction of Lonicerae Japinicae Flos and provided reference for the quality control method of terminal products from decoction of Lonicerae Japinicae Flos.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Lonicera/química , Ácido Clorogênico , Cromatografia Líquida de Alta Pressão , Flores/química , Extratos Vegetais/química , Extratos Vegetais/normas , Plantas Medicinais/química , Controle de Qualidade
20.
Zhongguo Zhong Yao Za Zhi ; 42(5): 817-822, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994519

RESUMO

The quality of Danshen extract granules on market is largely different from each other mainly due to the heterogeneous quality of raw materials of Salvia miltiorrhiza, various producing procedures and lack of good quality evaluation method. Formula granule and "standard decoction" have the same quality. In this paper, a systematic evaluation method for the quality of Danshen decoction was established from the perspective of "standard decoction", in order to explore the main factors affecting the quality uniformity of Danshen extract granules. Danshen standard decoction was prepared; then the fingerprint method was developed to determine the content of salvianolic acid B; and the main peaks in the fingerprint were identified with UPLC-QTOF/MS to clarify the chemical compositions of Danshen decoction. Three indexes were calculated to evaluate the stability of whole process, including the extraction ratio; transfer rate of index components and pH value. The results showed that the main components of Danshen decoction were phenolic acids, while the extraction rate, the transfer rate of salvianolic acid B and pH value were in a relatively stable level, and the similarity in the fingerprint of standard decoction was high, indicating that the preparation procedure was stable. The level of salvianolic acid B in the standard decoction was in a large range, which was mainly due to the difference in the quality of Salviae Miltiorrhizae Radix et Rhizoma.


Assuntos
Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Salvia miltiorrhiza/química , Benzofuranos/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Extratos Vegetais/química , Extratos Vegetais/normas , Raízes de Plantas/química , Controle de Qualidade , Rizoma/química
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