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1.
Microb Pathog ; 161(Pt A): 105275, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34732375

RESUMO

Hemolytic anemia and secondary hypoxia are characteristics of naturally occurring Babesia bigemina infection in cattle. The anemic phase comes with cardiovascular insufficiency due to hypoxia-induced system dysfunction; but to date there is no description of cardiac damage in the infected animals. Therefore, this study was undertaken to investigate hematological parameters, biomarkers of cardiac function and D-dimer in 13 cattle infected with B. bigemina which were unresponsive to standard treatment. The animals were necropsied and the cardiac tissue was examined for histopathologic alterations. A significant parasitemia burden-dependent increase in the cardiac biomarkers and D-dimer level were recorded in the infected cattle compared to the control animals. Thrombocytes count was also significantly lower in the infected animals than the control. Both macroscopic and microscopic hemorrhage, mononuclear infiltrates, and myocardial necrosis were the evident histopathologic findings. These findings suggest that B. bigemina infection can potentially induce cardiac dysfunction in cattle. Furthermore, mechanistic studies should be conducted to understand the mechanisms beyond cardiac complications.


Assuntos
Babesia , Babesiose , Doenças dos Bovinos , Animais , Biomarcadores , Bovinos , Parasitemia
2.
Vet Res Forum ; 12(3): 313-318, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34815842

RESUMO

Gastrointestinal motility disorders can occur as either increased or decreased movements. Studies have shown that herbal ingredients such as essential oils can modify the increase and decrease of gastrointestinal movements of ruminants. Cinnamaldehyde at room temperature is an oily yellow liquid which is obtained from the steam distillation of the oil of cinnamon bark. It bears carminative activity and gastrointestinal, antimicrobial, and vasodilatory effects. This study examined the effects of cinnamaldehyde on the contraction of circular smooth muscles of abomasal fundus and the antrum, duodenum, and ileum of healthy cows using an in vitro approach. The results indicated that cinnamaldehyde had relaxant effects on the basal tonus and contractions caused by barium chloride (BaCl2) and carbachol (CCh) in these tissues dependent upon concentration and the origin of the smooth muscle. These effects were more prominent in the ileal smooth muscle preparations than in other tissues. This substance in the smooth muscle preparations of the abomasal fundus not only had no significant effect on the basal tonus, but also significantly increased the contractions caused by barium chloride at low concentrations. Study of the mechanism of action showed that, similar to verapamil, cinnamaldehyde applied its relaxation effect by blocking the calcium channels. The results showed that cinnamaldehyde possessed a spasmolytic effect mediated through blockage of the calcium channels, which may provide a pharmacological base to its medicinal use for diarrhea and spasms.

3.
Vet Res Forum ; 12(3): 349-355, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34815847

RESUMO

Echocardiography is a non-invasive method of cardiac evaluation in most species. Echocardiographic reference parameters are poorly documented in sheep and not documented in Ghezel sheep. The purpose of this study was to determine reference ranges of normal echocardiographic parameters using two-dimensional and M-mode techniques in Ghezel sheep. In 15 healthy female Ghezel sheep aged between 15 and 18 months, echocardiography was performed in standing position from left and right parasternal approach focused at 3rd - 5th intercostal spaces using a 2.50 - 5.00 MHz phased array transducer. The following parameters were measured in two-dimensional echocardiography: left atrial diameter (LAD), mitral valve annulus (MVA), aortic sinus (AoS), aortic valve (AoV), pulmonary sinus (PuS), and pulmonary valve (PuV); and in M-mode echocardiography: interventricular septum (IVS), left ventricular internal diameter (LVID), left ventricular free wall (LVFW), right ventricle free wall (RVFW), right ventricle internal diameter (RVID). Calculated variables included the ratios LAD/Ao and Pu/AoS, and the fractional shortening (FS), end diastolic volume (EDV), end systolic volume (ESV), ejection fraction (EF), stroke volume (SV) and cardiac output (CO) of the left ventricle. In conclusion, echocardiographic parameters could be reliably assessed in Ghezel sheep and our study provided some normal echocardiographic reference ranges that might be useful in proper identification, visualization, and measurements of cardiac structures. Such findings could be useful to assess and to diagnose the specific heart diseases in sheep practice and also for experimental studies in sheep as an animal model used for research purposes in cardiovascular studies of human.

4.
Vet Res Forum ; 12(2): 175-183, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34345383

RESUMO

The present study was conducted to evaluate the effects of different doses of haloperidol (HP) on induction of oxidative stress in blood and liver cell degeneration in comparison with influences of HP pre-treatment on inflammatory process induced by intraperitoneal (IP) administration of lipopolysaccharide (LPS). One hundred twenty male albino Wistar rats were randomly divided into eight groups (15 in each), including: Control group, LPS group, three groups as HP administration in three divided doses (0.50, 1.00 and 2.00 mg kg-1), and three treatment groups that HP was administered in three doses (0.50, 1.00 and 2.00 mg kg-1) prior to LPS administration. Concentrations of malondialdehyde, activities of antioxidant enzymes including glutathione peroxidase, superoxide dismutase and also the levels of tumor necrosis factor-alpha and interleukin 1-beta were measured in blood and serum. In addition to liver histopathological changes evaluation, hepatic silent information regulator of transcription 1 (SIRT1) and phosphorylated-nuclear factor-κB (p-NF-κB) levels were quantitated. Our findings indicated that sole administration of HP (particularly higher doses) can induce oxidative stress in blood and cell degeneration in liver, while it can attenuate inflammatory process induced by LPS administration presumably via SIRT1 up-regulation and preventing the induction of p-NF-κB. The oxidative and degenerative effects of HP and its impact on inflammatory status were completely dose- dependent according to our results. The possible anti-inflammatory effects of HP may affect reparative mechanisms and hepatic cell degeneration. However, the influences of HP on immune system need further investigations and its higher doses should be administered cautiously especially in patients with immune system dysfunctions.

5.
Trop Anim Health Prod ; 53(2): 219, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33751256

RESUMO

Naturally occurring Babesia bigemina infection in cattle is associated with changes in the status of oxidative stress, trace elements, sialic acid, and cholinesterase activity in blood. However, to date there is no description of hepatic damage in the infected animals. More importantly, the majority of the above-mentioned causative factors are synthesized or stored in the liver. Therefore, this study was undertaken to evaluate biomarkers of hepatic function, paraoxonase-1 activity, and lipid profile in 13 cattle infected with B. bigemina which did not respond to standard treatment. The animals were necropsied and the histopathology of the liver and DNA damage of hepatocytes were examined. Blood analysis revealed a significant parasitemia burden-dependent increase in the activities of hepatic enzymes and total bilirubin and a decrease in albumin concentrations in the infected cattle compared to the control ones. Paraoxonase-1 activity was remarkably lower in the infected animals than the control. A significant decrease in the blood concentrations of total cholesterol, low density lipoprotein, and high density lipoprotein and a significant increase in the triglyceride concentration were observed in the infected animals. Severe oxidative damages were also recorded in the haptic tissue evidenced by significant alterations in the activities of antioxidant enzymes, suppression of total antioxidant capacity, and oxidation of biomolecules. Congestion of blood vessels, bile duct hyperplasia, and hepatocyte necrosis were the evident histopathologic findings. Our results revealed significant changes in the indices of liver function in the diseased cattle, leading to the conclusion that the parasite can potentially cause liver dysfunction.


Assuntos
Babesia , Babesiose , Doenças dos Bovinos , Hepatopatias , Animais , Arildialquilfosfatase , Bovinos , Lipídeos , Hepatopatias/veterinária , Estresse Oxidativo
6.
Acta Vet Hung ; 68(2): 147-153, 2020 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-33055306

RESUMO

An 11-year-old Hanoverian gelding used for jumping was evaluated for gait abnormalities and hoof problems in the hindlimbs. Clinical examinations revealed signs consistent with shivers. A thyroid gland enlargement was noticed, baseline serum thyroid hormone (TH) concentrations were low, and a low response to thyrotropin-releasing hormone administration was observed. Hypothyroidism was suspected. The horse was treated with levothyroxine for 1 year. TH concentrations returned to the normal range by week 4 of treatment. Thirty weeks after the initiation of levothyroxine therapy, the gait abnormality improved. Our findings suggest that the assessment of thyroid status and especially of the subclinical thyroid gland disorders in horses affected with shivering, as well as evaluation of the effects of levothyroxine on the improvement of clinical signs could be promising in establishing the aetiopathogenesis and/or treatment of shivering in horses.


Assuntos
Marcha , Doenças dos Cavalos/tratamento farmacológico , Hipotireoidismo/veterinária , Coxeadura Animal/tratamento farmacológico , Tremor por Sensação de Frio , Tiroxina/administração & dosagem , Animais , Cavalos , Hipotireoidismo/tratamento farmacológico , Masculino , Resultado do Tratamento
7.
Vet Res Forum ; 11(2): 191-193, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32782750

RESUMO

Various species of Trypanosoma parasites are known to infect several wild and domestic animals worldwide. A 7-year-old Holstein cow from Baneh, Kurdistan province, was examined by a private veterinarian due to anorexia and depression. Physical examination revealed fever, enlarged subscapular lymph node, and pale mucosa. Blood samples were taken for hemato-logical, parasitological, and PCR examination. The large Trypanosoma spp. was microscopically observed in a stained blood smear. Decreased red blood cells (RBCs) count, packed cell volume and hemoglobin concentration were observed through complete blood cell count. Nucleated RBCs were also found in this case. Species-specific PCR assay confirmed T. theileri infection. Treatment was performed subcutaneously with diminazene aceturate. The clinical signs were improved after two days. Two-month follow-up showed no recurrence. In conclusion, T. theileri is characterized by anemia and pyrexia in a cow. To our knowledge, the present case report describes the first molecular evidence of T. theileri in Kurdistan, West of Iran.

8.
Comp Immunol Microbiol Infect Dis ; 71: 101502, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-32505763

RESUMO

Equine theileriosis due to Theileria equi is probably the most widespread and pathogenic disease of equines, which comes with major cardiac and renal complications. This study was undertaken to investigate the biomarkers of cardiac and renal functions in horses infected with T. equi and determine the association between these parameters and the level of parasitemia. Giemsa-stained blood smears from 300 horses with ages of 3-4 years old were examined for detection of T. equi on erythrocytes. Moreover, multiplex PCR was employed for confirmation of the diagnosis in the 28 positive cases. Based on the rate of red blood cell infection, the infected animals were subdivided into horses with low (n = 9), moderate (n = 13) and high (n = 6) parasitemia. The concentrations of urea, creatinine, cystatin-C, cardiac troponin I (cTn-I), homocysteine (Hcy), myocardial fractions of creatine kinase (CK-MB) and d-dimer were determined in control (healthy) horses (n = 20) and the infected animals. The results revealed that both the renal (urea, creatinine and cystatin-C) and the cardiac (cTn-I, Hcy, CK-MB and d-dimer) biomarkers increased in a parasitemia burden-dependent pattern. However, urea, creatinine, cTn-I and d-dimer levels were not significantly influenced in the horses infected with low rate (<1 %) of parasitemia (p> 0.05). Moreover, all of the biomarkers were significantly and positively associated with the parasitemia (R2> 0.5). In conclusion, T. equi infection was related to cardiac and renal complications evidenced by increase in the levels of biomarkers and evaluation of these indices may have promise for early diagnosis of the complications.

9.
Comp Immunol Microbiol Infect Dis ; 71: 101503, 2020 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-32505767

RESUMO

Babesia bigemina infection (also known as Texas fever) is reported as the most prevalent and main causative agent of bovine babesiosis, worldwide. The current study was undertaken to assess indicators of oxidative stress including activities of antioxidant enzymes and total antioxidant capacity (TAC), oxidation status of biomolecules and serum levels of trace elements as well as indicators of inflammation including sialic acid (SA) contents and cholinesterase activity in cattle naturally infected with B. bigemina. An infected group comprised of 20 crossbred Holstein cattle (3-4 years old) were diagnosed to be positive by both microscopy and nested PCR assay. The infected animals were subdivided into two groups according to their parasitemia rates (<20 % and>20 %). Furthermore, 10 healthy cattle were included as the control. The infection caused severe anemia in a parasitemia-burden dependent fashion. The activities of catalase and glucose-6-phosphate dehydrogenase as well as the levels of TAC, zinc, selenium, copper and manganese were significantly decreased as the parasitemia increased, accordingly. However, the activity of superoxide dismutase as well as the levels of malondialdehyde, protein carbonylation, DNA damage and iron, were significantly elevated in a parasitemeia-burden dependent manner. Additionally, glutathione peroxidase activity was significantly elevated with the lower rate of parasitemia, but the higher rate had no significant effect as compared to control. Moreover, total, protein and lipid binding SA contents were significantly increased but the activities of acetylcholinesterase and butyrylcholinesterase were significantly reduced, parasitemia dependently. Conclusively, the infection was remarkably associated with the induction of anemia, oxidative stress and inflammation.

10.
Biol Trace Elem Res ; 194(2): 401-409, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31267443

RESUMO

Unlike in human medicine, information on the platelet selenium (Se) concentration and glutathione peroxidase (GPx) activity, as potential biomarkers of Se status, is lacking in the field of veterinary medicine. The aims of this study were to compare the effects of sodium selenite (SeS) and Se nanoparticles (SeN) on platelet Se indices, and compare the results with those of conventional indices of Se status in sheep. Eighteen lambs with marginal Se status were assigned to a control (CON) group and two Se-treated groups administered either a single dose of Se at 0.055 mg/kg BW subcutaneously as SeS or SeN. The Se concentration and GPx activity in plasma, erythrocytes, whole blood, and platelets were assessed at 14 time points during 90 days. In Se-treated groups, Se concentrations and GPx activity increased significantly in plasma, whole blood, erythrocytes, and platelets. However, the responses in SeS group often started and peaked earlier and, in almost all time points, were more pronounced than the SeN group (P < 0.001), indicating that SeS compared to SeN was more biopotent in raising Se biomarkers. Se indices in platelets started and peaked later than those of short-term (in plasma) and earlier than those of long-term indices (in erythrocytes) indicating that platelet indices can serve as intermediate-term biomarkers of Se status. In conclusion, platelet indices of Se are promising proxy for currently used indices of Se status in sheep, regardless of Se source.


Assuntos
Nanopartículas , Selênio , Animais , Eritrócitos , Glutationa Peroxidase , Selênio/farmacologia , Ovinos , Selenito de Sódio/farmacologia
11.
Iran J Parasitol ; 14(2): 347-351, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31543925

RESUMO

A 4-year-old male one-humped camel (Camelus dromedarius) was referred to Veterinary Teaching Hospital of Urmia University, Iran in 2017 with anorexia, weakness, depression and pale mucosa. Decreased red blood cell count, packed cell volume and hemoglobin concentration were detected by complete blood cell count. In Giemsa-stained peripheral blood smears Trypanosoma spp. trypomastigotes scattered between erythrocytic spaces and Mycoplasma-like organisms were observed attached to the surface of erythrocytes. Species-specific PCR assay confirmed T. evansi and Candidatus Mycoplasma haemolamae (CMhl) co-infection. Administration of diminazene aceturate, oxytetracycline 20%, flunixin meglumine and phosphorus-vitamin B12 were not effective in treatment. Hemoplasmosis should be considered as an important differential diagnosis of conditions associated with hemolytic anemia in camel.

12.
Iran J Pharm Res ; 18(1): 320-327, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31089366

RESUMO

Pseudomonas aeruginosa is one of the most common causes of keratitis. The current study was done to evaluate the therapeutic effects of antibacterial combinations with Silver nanoparticles (Ag-NPs) and Ciprofloxacin in experimental Pseudomonas keratitis. Sixty four New Zealand rabbits were prepared. All rabbits were randomly categorized into eight groups (each group containing eight rabbits): Control +, Control -, Ciprofloxacin, Ag-NPs, Ciprofloxacin plus Betamethasone, Ag-NPs plus Betamethasone, Ciprofloxacin plus Ag-NPs, and Ciprofloxacin plus Ag-NPs plus Betamethasone. Twelve hours after bacterial inoculation into the cornea, the eyes were examined daily to evaluate the number of days of ocular discharge and blepharospasm. Also, after 108 and 204 h, first grading of corneal opacity was done and then four rabbits of each groups were euthanized for bacterial count. The results showed that the means of days of blepharospasm, ocular discharge, and bacterial counts (log CFU mL-1) were significantly different in the treatment groups at 108 and 204 h (P <0.0005, ANOVA). According to Tukey's test, Ciprofloxacin plus Ag-NPs plus Betamethasone group was significantly less than Control +, Ag-NPs, and Ag-NPs plus Betamethasone groups for these variables (P < 0.05). The mean rank of opacity scores was significantly different between treatment groups (P = 0.01, Kruskal-Wallis). Mann-Whitney U-test revealed that Ciprofloxacin plus Ag-NPs plus Betamethasone group had significantly better score than Control +, Ag-NPs, and Ag-NPs plus Betamethasone groups (P < 0.05). It seems Ag-NPs can be an appropriate adjuvant for Ciprofloxacin, but due to the results they can't be an alternative for Ciprofloxacin to treat Pseudomonas keratitis.

13.
Ann Parasitol ; 64(3): 235-240, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30316220

RESUMO

Dictyocaulus arnfieldi is a lungworm commonly found in equids; however, relatively little is known about it. The aim of the present study was to establish the prevalence of equine lungworms in naturally-infected working and sporting horses, donkeys and mules in rural areas of Urmia, northwest Iran. The fecal samples were collected from 299 working horses, 57 sporting horses, 66 donkeys and 37 mules during the period March 2014 to June 2016. The collected fecal samples were processed within 48 hours following rectal sampling. The larval count/g (LPG) of feces was determined using the Baermann technique. The larvae were identified morphologically, and the arithmetic mean of the LPG was calculated at each sampling point. The overall prevalence of lungworm in all equine species was found to be 19.20%., with specific values of 15.71%, 0%, 31.81% and 24.32% being found in working horses, sporting horses, donkeys and mules, respectively. The working horses, donkeys and mules were also found to be heavily infected with Dictyocaulus arnfieldi and in addition to donkeys and mules, working horses are considered natural hosts for lungworm, and this has effects on both the epidemiology of parasitic infections and performance of the equids.


Assuntos
Infecções por Dictyocaulus , Dictyocaulus , Doenças dos Cavalos , Cavalos , Nematoides , Animais , Dictyocaulus/isolamento & purificação , Equidae , Fezes , Doenças dos Cavalos/parasitologia , Cavalos/parasitologia , Irã (Geográfico) , Inquéritos e Questionários
14.
Trop Anim Health Prod ; 50(8): 1851-1858, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29948777

RESUMO

Lumpy skin disease (LSD) is a devastating viral disease of cattle which has recently spread from Africa into the countries of the Middle East. The aim of the present study was to investigate the relationships among lumpy skin disease viruses (LSDV) isolated from different regions of Iran and the origin and spread of these viruses. In this study, a total of 234 blood samples from clinically affected animals from four provinces in the northwest of Iran were screened for LSDV using polymerase chain reaction (PCR). From 80 positive samples for LSDV detected by PCR, the partial P32 gene (759 bp) of 12 isolates were sequenced and phylogenetically analyzed. LSD viruses were grouped in three subclusters with an overall 97.1-100% nucleotide identity. LSDVs isolated from Gilan showed lowest nucleotide identity with the other LSDVs. Four isolates of LSDV including KO-1, EA-1, EA-3, and WA-3 showed 100% similarity with each other and also with the Neethling strain. Phylogenetic analysis indicated that the identified LSDVs were closely related to each other and had high-sequence homology with other LSDV isolates from Africa. It was concluded that LSD outbreak probably occurred in the northwest of Iran by LSDVs entering the country from Iraq and P32 nucleotide sequence information obtained in the present study is a valuable resource in understanding the genetic nature and molecular epidemiology of local LSDV isolates which can be used for future vaccine development based on the circulating strains in the region.


Assuntos
Doença Nodular Cutânea/epidemiologia , Vírus da Doença Nodular Cutânea/genética , Reação em Cadeia da Polimerase/veterinária , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Análise por Conglomerados , DNA Viral/análise , Surtos de Doenças/veterinária , Genótipo , Irã (Geográfico)/epidemiologia , Filogenia , Homologia de Sequência
15.
Vet Res Forum ; 8(2): 115-120, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28785386

RESUMO

Displacement of the abomasum is a common disease of the gastrointestinal tract in dairy cattle. Abomasal displacement has been associated with abomasal hypomotility. Therefore, it is necessary to identify effective therapeutic agents that stimulate abomasal motility in cattle. Yarrow (Achillea millefolium) is traditionally used as a folk remedy for treatment of human gastrointestinal complaints in the northwest of Iran. This study investigated the effects of A. millefolium extracts on abomasal smooth muscle preparations from healthy cattle. The A. millefolium extracts (3 to3000 mg L-1) contracted the isolated of smooth muscle in a concentration-dependent manner, with an effective threshold concentration of 30 mg L-1 (p < 0.05). The strongest contraction by A. millefolium aqueous extracts in a concentration of 3000 mg L-1 was observed and amounted to 124.90 ± 10.80% of the control treatment. This action was unaffected by pretreatment with hexamethonium and indomethacin, but strongly reduced by verapamil, atropine and 4-DAMP. The inhibiting effect of 4-DAMP and atropine suggesting that the effect of A. millefolium extracts is mediated at least partly by M3 muscarinic acetylcholine receptor. The results suggested that A. millefolium has the potential prokinetic effect that may prevent or alleviate dysfunctions of gastrointestinal motility.

16.
J Trace Elem Med Biol ; 42: 129-141, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28595785

RESUMO

The cardio-protective effects of zinc oxide nanoparticles (Zn NPs) against diabetes-induced cardiopathy were evaluated and compared with zinc sulfate (ZnSO4). A total of 120 Wistar rats were randomly categorized as healthy and diabetic groups. Then, the 2 groups were classified in 5 subgroups. The animals received oral supplementations containing different Zn NP (ie, doses of 1, 3, and 10mg/kg) and ZnSO4 (30mg/kg) concentrations over 8 weeks. Blood and cardiac tissue samples were collected in the different time intervals and subjected to biochemical and histopathological analysis. Zn NPs showed dual effects, as its middle dose played protective role and recovered cardiac damages evidenced by significant reduction of serum cholesterol, HDL-cholesterol, lipoprotein (a), atherogenic index, TNF-α, cardiac MDA, B-type natriuretic peptide and caspase-3 activity. Apoptosis indices and histopathological features also were improved. However, the highest dose was found to be toxic and resulted in aggravation of the injuries. Another interesting finding is the ability of the higher doses of Zn-NPs (3 and 10mg/kg) to elevate cardiac zinc levels above the normal range in healthy animal. ZnSO4 also helped to recuperation of the damages, but the middle dose of Zn NPs was more efficient as compared to ZnSO4. Conclusively, Zn NPs have the potential for Zn delivery in diabetic patients.


Assuntos
Cardiotônicos/uso terapêutico , Diabetes Mellitus Experimental/tratamento farmacológico , Nanopartículas/uso terapêutico , Óxido de Zinco/uso terapêutico , Sulfato de Zinco/uso terapêutico , Animais , Apoptose/efeitos dos fármacos , Aterosclerose/sangue , Aterosclerose/patologia , Cardiotônicos/farmacologia , Caspase 3/sangue , Caspase 3/metabolismo , Colesterol/sangue , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/patologia , Queratina-18/metabolismo , Masculino , Malondialdeído/metabolismo , Nanopartículas/ultraestrutura , Ratos Wistar , Estreptozocina , Fator de Necrose Tumoral alfa/sangue , Difração de Raios X
17.
Vet Res Forum ; 8(1): 23-28, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28473893

RESUMO

The microorganisms have been noted as the main cause of delayed wound healing. The most common pathogen causing the wound infections is Staphylococcus aureus. Silver nanoparticles (AgNPs) show ample antibacterial activities. In the present study, the effect of AgNPs on mouse wounds inoculated with S. aureus was investigated. Sixty male mice (20 to 30 g) were anesthetized, full-thickness skin wounds were made on their back and then the bacterial suspension was added to each wound bed. Treatments were administered on wound bed topically including gentamicin (8 mg kg-1), AgNPs (0.08 mg kg-1, 0.04 mg kg-1 and 0.02 mg kg-1) and normal saline in the control group. Wound healing was monitored macroscopically by taking digital photographs on days 0, 7, 14 and 21 of the experiment. Topical application of gentamicin and AgNPs (0.08 and 0.04 mg kg-1) significantly increased the rate of wound healing more than treatment with AgNPs at a dose of 0.02 mg kg-1and normal saline. The presence of silver nanoparticles in AgNPs groups (especially 0.08 mg kg-1) improved wound appearance better than other groups without silver nanoparticles (gentamicin and control groups) and led to lesser wound scars. According to data analysis, healing rate of treated mice with gentamicin and AgNPs (0.08 mg kg-1) was significantly (p < 0.001) faster than treated mice with other AgNPs doses and normal saline. The results of current study introduced an in vivo nanosilver accelerating effects on the treatment of on S. aureus infected skin wounds.

18.
J Arthropod Borne Dis ; 9(2): 246-52, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26623436

RESUMO

BACKGROUND: Small ruminants' babesiosis caused by Babesia ovis, is transmitted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. This study was carried out to to estimate the infection rate of B. ovis in sheep and goats by PCR. We have analysed risk factors that might influence infection of sheep and goats with B. ovis. METHODS: A total 402 blood samples were examined microscopically for the presence of Babesia infection. All samples were tested by PCR. During sampling, whole body of each animal and farm dogs was examined for the presence of ticks. RESULTS: Forty-two animals (10.4%) were positive for Babesia spp. upon microscopic examination, whereas 67 animals (16.7%) yielded the specific DNA for B. ovis of which 52 animals were sheep and 15 animals were goats. Twenty-nine farms (72.5%) were found positive for B. ovis. The percentage of positive animals in each location varied from 13 % to 20 %. The relative risk of the presence of ticks in sheep and goats (P< 0.01) and farm dogs (P< 0.01) for PCR- positive results for B. ovis in sheep and goats was found 3.8 and 2.9, respectively. A total of 747 ticks identified as Rhipicephalus bursa, R. sanguineus and R. turanicus on the basis of morphological features. CONCLUSION: Other animal species besides dogs may also be risk factors for babesiosis in sheep and goats. Also, R. bursa may play an important role as a vector of the parasite in Iran.

19.
Biol Trace Elem Res ; 168(1): 74-81, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25900578

RESUMO

Several biomarkers have been used to evaluate selenium (Se) status in livestock. However, there is no report on the potential usefulness of the Se indices of platelets in diagnosis of Se deficiency in large animals. In the current study, Se concentration and glutathione peroxidase (GPx) activity in platelets of 38 healthy and 142 Se-deficient ewes were assessed, and their correlation with plasma Se concentration, plasma GPx activity, whole blood Se concentration, and erythrocyte GPx activity was determined. Receiver operating characteristic (ROC) curve analysis was used to determine the optimal cutoff values of Se concentration and GPx activity of the platelets and to summarize the diagnostic performance of these biomarkers. In Se-deficient ewes, consistent with other indices, Se concentration and GPx activity in platelets were significantly lower than those of the healthy ewes. There was a positive significant correlation between Se concentration and GPx activity in platelets with plasma Se concentration, whole blood Se concentration, and erythrocyte GPx activity. Based on the ROC curve analysis, the best cutoff value to predict inadequate plasma selenium concentration was ≤0.0055 attogram/platelet for the platelet Se concentration, with a sensitivity of 100.0 %, specificity of 92.4 %, and AUC of 0.94. For platelet GPx activity, the cutoff value was ≤203.6 U/g protein with a sensitivity of 97.4 %, specificity of 77.7 %, and AUC of 0.90. The results of this study suggested that the platelet Se concentration and GPx activity can be considered a reliable and valid intermediate-term surrogate parameter in assessment of dietary Se intake in sheep.


Assuntos
Plaquetas/química , Eritrócitos/química , Selênio/sangue , Selênio/deficiência , Animais , Biomarcadores/análise , Feminino , Glutationa Peroxidase/sangue , Humanos , Estado Nutricional , Ovinos
20.
Vet Res Forum ; 6(3): 257-60, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26893818

RESUMO

A 5-day-old female Holstein calf was necropsied because of lethargy, recumbency and anorexia. At necropsy, multiple gross defects were evident in several organs, including unclosed sutures of skull bones, asymmetrical orbits, doming of the skull bones, hydrocephalus, hydranencephaly, cleft palate, brachygnathia, ventricular septal defect, mitral valve dysplasia and rudimentary lungs. On microscopic examination, pulmonary hypoplasia was characterized by reduced number of alveoli, replacement of peri-bronchiolar smooth muscles with connective tissue and small masses of undeveloped cartilage around the small airways. The present report is the first description of the congenital pulmonary hypoplasia accompanied by numerous malformations in Holstein breed.

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