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1.
J Agric Food Chem ; 67(38): 10577-10586, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31490682

RESUMO

Root and rhizosphere is important for phosphorus (P) uptake in rice plants. However, little is known about the detailed regulation of irrigation regimes, especially frequently alternate wetting and drying (FAWD), on P usage of rice plants. Here, we found that compared with normal water and P dose, FAWD with a reduced P dose maintained the grain yield in two rice varieties. Compared to rice variety Gaoshan1, rice variety WufengyouT025 displayed a higher grain yield, shoot P content, rhizosphere acid phosphatase activity, abundance of bacteria, and bacterial acid phosphatase gene of rhizosphere. Moreover, the FAWD regime may increase the abundance of bacteria with acid phosphatase activity to release available phosphorus in the rhizosphere, which is associated with rice varieties. Our results suggest that an optimized management of irrigation and phosphorous application can enhance both water and phosphorus use efficiency without sacrificing the yield, which may contribute significantly to sustainable agriculture production.


Assuntos
Irrigação Agrícola/métodos , Produção Agrícola/métodos , Oryza/crescimento & desenvolvimento , Fósforo/metabolismo , Microbiologia do Solo , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Produção Agrícola/instrumentação , Fertilizantes/análise , Microbiota , Oryza/classificação , Oryza/metabolismo , Oryza/microbiologia , Rizosfera , Água/metabolismo
2.
ACS Appl Mater Interfaces ; 11(25): 22628-22636, 2019 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-31244026

RESUMO

Microwave absorption materials (MAMs) with lightweight density and ultrabroad-band microwave absorption performance are urgently needed in advanced MAMs, which are still a big challenge and have been rarely achieved. Here, a new wide bandwidth absorption model was designed, which fuses the electromagnetic resonance loss ability of a periodic porous structure in the low-frequency range and the dielectric loss ability of dielectric materials in the high-frequency range. Based on this model, a lightweight porous cellulose nanofiber (CNF)/carbon nanotube (CNT) foam consisting of a cellular vertical porous architecture with the macropore diameters between 30 and 90 µm and a nanoporous architecture at a scale of 1.7-50 nm was obtained by an ice-template method using CNTs and CNFs as "building blocks". Benefiting from the unique architecture, the effective absorption bandwidth reaches 29.7 GHz, and its specific microwave absorption performance exceeds 80,000 dB·cm-2·g-1, which far surpasses those of the MAMs previously reported, including all CNT-based composites. Moreover, the CNF/CNT foam possesses ultralow density (9.2 mg/cm3) and strong fatigue resistance, all coming from the well-interconnected porous structure and the strong hydrogen bonds among CNF-CNF and CNF-CNT molecular chains.

3.
Molecules ; 19(5): 5913-24, 2014 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-24815310

RESUMO

Hydrogen peroxide (H2O2) and nitric oxide (NO) are key signaling molecules in cells whose levels are increased in response to various stimuli and are involved in plant secondary metabolite synthesis. In this paper, the roles of H2O2 and NO on salvianolic acid B (Sal B) production in salicylic acid (SA)-induced Salvia miltiorrhiza cell cultures were investigated. The results showed that H2O2 could be significantly elicited by SA, even though IMD (an inhibitor of NADPH oxidase) or DMTU (a quencher of H2O2) were employed to inhibit or quench intracellular H2O2. These elicited H2O2 levels significantly increased NO production by 1.6- and 1.46 fold in IMD+SA and DMTU+SA treatments, respectively, and induced 4.58- and 4.85-fold Sal B accumulation, respectively. NO was also markedly elicited by SA, in which L-NNA (an inhibitor of NO synthase) and cPTIO (a quencher of NO) were used to inhibit or quench NO within cells, and the induced NO could significantly enhance H2O2 production by 1.92- and 1.37-fold in L-NNA+SA and cPTIO+SA treatments, respectively, and 3.27- and 1.50-fold for Sal B accumulation, respectively. These results indicate that elicitation of SA for either H2O2 or NO was independent, and the elicited H2O2 or NO could act independently or synergistically to induce Sal B accumulation in SA-elicited cells.


Assuntos
Benzofuranos/metabolismo , Salvia miltiorrhiza/metabolismo , Transdução de Sinais/genética , Benzofuranos/química , Técnicas de Cultura de Células , Peróxido de Hidrogênio/química , Peróxido de Hidrogênio/metabolismo , Óxido Nítrico/química , Óxido Nítrico/farmacologia , Óxido Nítrico Sintase/metabolismo , Ácido Salicílico/química , Ácido Salicílico/metabolismo , Salvia miltiorrhiza/citologia , Salvia miltiorrhiza/genética
4.
Sheng Wu Gong Cheng Xue Bao ; 29(12): 1836-46, 2013 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-24660631

RESUMO

In order to study the effects of Ca2+ in the biosynthesis of salvianolic acid B (Sal B) induced by salicylic acid (SA) in the young seedlings of Salvia miltiorrhiza, we used confocal laser scanning microscopy and high performance liquid chromatography to measure the change of relative fluorescence intensity of Ca2+ and the contents of Sal B induced by SA before and after the application of extracellular calcium channel inhibitors (VP and LaCl3), intracellular calcium channel inhibitor (LiCl), as well as intracellular calmodulin antagonist (TFP). SA could induce the calcium burst, and the Ca2+ peak could last to 2-3 min in the guard cells of S. miltiorrhiza, which prompted the biosynthesis of Sal B after the Ca2+ burst. Both Vp or LaCl3, and LiCl or TFP could inhibit the burst of Ca2+ and the biosynthesis of Sal B. The above results demonstrated that Ca2+ from the extracellular and the intracellular calcium store regulate the biosynthesis of Sal B elicited by salicylic acid in S. miltiorrhiz young seedlings.


Assuntos
Benzofuranos/metabolismo , Cálcio/metabolismo , Ácido Salicílico/farmacologia , Salvia miltiorrhiza/metabolismo , Plântula/metabolismo , Folhas de Planta/metabolismo , Transdução de Sinais
5.
Zhongguo Zhong Yao Za Zhi ; 38(20): 3424-31, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24490547

RESUMO

OBJECTIVE: To investigate the effect of intracellular and extracellular Ca2+ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. METHOD: Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid (SA), and then use the optimal concentration of SA to investigate the effects of extracellular Ca2+ channel inhibitors Verapamil, LaCl3, intracelluar calmodulin antagonist TFP and intracelluar Ca2+ channel inhibitors LiCl on the biosynthesis of RA and related enzymes. RESULT: SA increased the accumulation of RA and the activities of PAL and TAT, especially the SA of 2 mmol x L(-1) after 24 h. SA improved the accumulation of RA to (40.51 +/- 2.16) mg x g(-1), which was 1.97 times than that of control, and the activities of PAL, TAT were 1.42 times and 1.29 times than those of the control. However, Vp, LaCl3, TFP, LiCl inhibited the effects of SA evidently. CONCLUSION: Ca2+ plays a key role in the regulation of the induction process.


Assuntos
Cálcio/metabolismo , Cinamatos/metabolismo , Depsídeos/metabolismo , Ácido Salicílico/metabolismo , Salvia miltiorrhiza/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Salvia miltiorrhiza/genética , Salvia miltiorrhiza/crescimento & desenvolvimento , Plântula/genética , Plântula/crescimento & desenvolvimento , Plântula/metabolismo
6.
Sheng Wu Gong Cheng Xue Bao ; 28(11): 1359-69, 2012 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-23457788

RESUMO

We studied the influence of the concentration of Ca2+ (0-50 mmol/L) in culture medium on the synthesis of rosmarinic acid (RA) and related enzymes in Salvia miltiorrhiza suspension cultures. Using verpamil (VP, a calcium channel antagonist) and ionophore A23187, we studied the mechanism of secondary metabolites of Salvia miltiorrhiza suspension cultures influenced by the concentration of Ca2+ in the culture medium. The synthesis of intracellular RA in 6-day incubation was significantly dependent on the medium Ca2+ concentration. At the optimal Ca2+ concentration of 10 mmol/L, a maximal RA content of 20.149 mg/g biomass dry weight was reached, which was about 37.3% and 20.4% higher than that at Ca2+ concentrations of 1 and 3 mmol/L, respectively. The variation of the activity of PAL and TAT, two key enzymes of the two branches of RA, could be affected by the concentration of Ca2+ in culture medium. The change of their activity occurred prior to the accumulation of RA, which suggested both of the key enzymes be involved in the synthesis of RA. Meanwhile, the enzymatic action of PAL was more distinct than TAT. The treatment of VP and A23187, respectively, indicated that the influence of RA affected by the concentration of Ca2+ in the culture medium was accomplished by the intracellular Ca2+, and the flow of Ca2+ from the extracellular to the intracellular environment could also participate in this process.


Assuntos
Cálcio/farmacologia , Cinamatos/metabolismo , Depsídeos/metabolismo , Salvia miltiorrhiza/química , Salvia miltiorrhiza/enzimologia , Meios de Cultura , Técnicas de Cultura/métodos , Fenilalanina Amônia-Liase/metabolismo , Salvia miltiorrhiza/crescimento & desenvolvimento , Tirosina Transaminase/metabolismo
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