Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Mais filtros

Intervalo de ano de publicação
Biodegradation ; 32(2): 145-163, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33586077


Soil is the recipient of organic pollutants as a consequence of anthropogenic activities. Hydrocarbons are contaminants that pose a risk to human and environmental health. Bioremediation of aging contaminated soils is a challenge due to the low biodegradability of contaminants as a result of their interaction with the soil matrix. The aim of this work was to evaluate the effect of both composting and the addition of mature compost on a soil chronically contaminated with hydrocarbons, focusing mainly on the recovery of soil functions and transformations of the soil matrix as well as microbial community shifts. The initial pollution level was 214 ppm of polycyclic aromatic hydrocarbons (PAHs) and 2500 ppm of aliphatic hydrocarbons (AHs). Composting and compost addition produced changes on soil matrix that promoted the release of PAHs (5.7 and 15 % respectively) but not the net PAH elimination. Interestingly, composting stimulated AHs elimination (about 24 %). The lack of PAHs elimination could be attributed to the insufficient PAHs content to stimulate the microbial degrading capacity, and the preferential consumption of easily absorbed C sources by the bacterial community. Despite the low PAH catabolic potential of the aging soil, metabolic shift was driven by the addition of organic matter, which could be monitored by the ratio of Proteobacteria to Actinobacteria combined with E4/E6 ratio. Regarding the quality of the soil, the nutrients provided by the exogenous organic matter contributed to the recovery of the global functions and species diversity of the soil along with the reduction of phytotoxicity.

Compostagem , Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Biodegradação Ambiental , Humanos , Hidrocarbonetos , Hidrocarbonetos Policíclicos Aromáticos/análise , Solo , Microbiologia do Solo , Poluentes do Solo/análise
Environ Sci Pollut Res Int ; 21(12): 7548-56, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24595755


Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous compounds with carcinogenic and/or mutagenic potential. To address the limitations of individual remediation techniques and to achieve better PAH removal efficiencies, the combination of chemical and biological treatments can be used. The degradation of phenanthrene (chosen as a model of PAH) by persulfate in freshly contaminated soil microcosms was studied to assess its impact on the biodegradation process and on soil properties. Soil microcosms contaminated with 140 mg/kgDRY SOIL of phenanthrene were treated with different persulfate (PS) concentrations 0.86-41.7 g/kgDRY SOIL and incubated for 28 days. Analyses of phenanthrene and persulfate concentrations and soil pH were performed. Cultivable heterotrophic bacterial count was carried out after 28 days of treatment. Genetic diversity analysis of the soil microcosm bacterial community was performed by PCR amplification of bacterial 16S rDNA fragments followed by denaturing gradient gel electrophoresis (DGGE). The addition of PS in low concentrations could be an interesting biostimulatory strategy that managed to shorten the lag phase of the phenanthrene biological elimination, without negative effects on the physicochemical and biological soil properties, improving the remediation treatment.

Biodegradação Ambiental , Fenantrenos/química , Microbiologia do Solo , Poluentes do Solo/química , Argentina , Citratos/química , DNA Ribossômico/análise , Eletroforese em Gel de Gradiente Desnaturante , Concentração de Íons de Hidrogênio , Oxirredução , Fenantrenos/análise , Reação em Cadeia da Polimerase , Citrato de Sódio , Compostos de Sódio/química , Poluentes do Solo/análise , Sulfatos/química
Microb Ecol ; 59(2): 266-76, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19609598


The present study describes the phenanthrene-degrading activity of Sphingomonas paucimobilis 20006FA and its ability to promote the bioavailability of phenanthrene. S. paucimobilis 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). The accumulation of 1-hydroxy-2-naphthoic acid and salicylic acid as major phenanthrene metabolites and the capacity of the strain to grow with sodium salicylate as the sole source of carbon and energy indicated that the S. paucimobilis 20006FA possesses a complete phenanthrene degradation pathway. However, under the studied conditions, the strain was able to mineralize only the 10% of the consumed phenanthrene. Investigations on the cell ability to promote bioavailability of phenanthrene showed that the S. paucimobilis strain 20006FA exhibited low cell hydrophobicity (0.13), a pronounced chemotaxis toward phenanthrene, and it was able to reduce the surface tension of mineral liquid medium supplemented with phenanthrene as sole carbon source. Scanning electron micrographs revealed that: (1) in suspension cultures, cells formed flocks and showed small vesicles on the cell surface and (2) cells were also able to adhere to phenanthrene crystals and to produce biofilms. Clearly, the strain seems to exhibit two different mechanisms to enhance phenanthrene bioavailability: biosurfactant production and adhesion to the phenanthrene crystals.

Fenantrenos/metabolismo , Microbiologia do Solo , Sphingomonas/metabolismo , Biodegradação Ambiental , Disponibilidade Biológica , Carbono/metabolismo , Meios de Cultura , Interações Hidrofóbicas e Hidrofílicas , Microscopia Eletrônica de Varredura , Naftóis/metabolismo , Ácido Salicílico/metabolismo , Sphingomonas/ultraestrutura , Tensoativos/metabolismo
Biodegradation ; 20(1): 95-107, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18604587


The combined effect of phenanthrene and Cr(VI) on soil microbial activity, community composition and on the efficiency of bioremediation processes has been studied. Biometer flask systems and soil microcosm systems contaminated with 2,000 mg of phenanthrene per kg of dry soil and different Cr(VI) concentrations were investigated. Temperature, soil moisture and oxygen availability were controlled to support bioremediation. Cr(VI) inhibited the phenanthrene mineralization (CO(2) production) and cultivable PAH degrading bacteria at levels of 500-2,600 mg kg(-1). In the bioremediation experiments in soil microcosms the degradation of phenanthrene, the dehydrogenase activity and the increase in PAH degrading bacteria counts were retarded by the presence of Cr(VI) at all studied concentrations (25, 50 and 100 mg kg(-1)). These negative effects did not show a correlation with Cr(VI) concentration. Whereas the presence of Cr(VI) had a negative effect on the phenanthrene elimination rate, co-contamination with phenanthrene reduced the residual Cr(VI) concentration in the water exchangeable Cr(VI) fraction (WEF) in comparison with the soil microcosm contaminated only with Cr(VI). Clear differences were found between the denaturing gradient gel electrophoresis (DGGE) patterns of each soil microcosm, showing that the presence of different Cr(VI) concentrations did modulate the community response to phenanthrene and caused perdurable changes in the structure of the microbial soil community.

Bactérias/metabolismo , Cromo/metabolismo , Fenantrenos/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Biodegradação Ambiental
FEMS Microbiol Ecol ; 53(2): 305-16, 2005 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-16329950


Qualitative and quantitative changes of microbial communities in soil microcosms during bioremediation were determined throughout one year. The soil was contaminated with 0%, 2.5%, 5%, 10% (wt/wt) of petrochemical sludge containing polynuclear aromatic hydrocarbons. We analyzed the hydrocarbon concentration in the microcosms, the number of cultivable bacteria using CFU and most probable number assays, the community structure using denaturing gradient gel electrophoresis, and the metabolic activity of soil using dehydrogenase activity and substrate-induced respiration assays. After one year of treatment, the chemical analysis suggested that the hydrocarbon elimination process was over. The biological analysis, however, showed that the contaminated microcosms suffered under long-term disturbance. The number of heterotrophic bacteria that increased after sludge addition (up to 10(8)-10(9) cells ml(-1)) has not returned to the level of the control soil (2-6 x 10(7) cells ml(-1)). The community structure in the contaminated soils differed considerably from that in the control. The substrate-induced respiration of the contaminated soils was significantly lower (approximately 10-fold) and the dehydrogenase activity was significantly higher (20-40-fold) compared to the control. Changes in the community structure of soils depended on the amount of added sludge. The species, which were predominant in the sludge community, could not be detected in the contaminated soils.

Biodegradação Ambiental , Petróleo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Hidrocarbonetos/análise , Oxirredutases/metabolismo , Dinâmica Populacional , Eliminação de Resíduos
In. AIDIS Argentina. Es tiempo de convertir nuestras acciones en proyectos. Mendoza, AIDIS, 2000. p.12, Ilus, tab.
Monografia em Espanhol | BINACIS | ID: bin-140896
In. AIDIS Argentina. Es tiempo de convertir nuestras acciones en proyectos. Mendoza, AIDIS, 2000. p.10, Ilus, tab.
Monografia em Espanhol | BINACIS | ID: bin-140895
In. AIDIS Argentina. Es tiempo de convertir nuestras acciones en proyectos. Mendoza, AIDIS, 2000. p.13, Ilus, tab.
Monografia em Espanhol | BINACIS | ID: bin-140894


Presenta la aplicación de un esquema de ensayo de tratabilidad en tres niveles para evaluar y diseñar una técnica de bio-remediación para el tratamiento de barros de fondo de separadores API con un alto contenido de hidrocarburos aromáticos polinucleares generados en el polo petroquímico Ensenada. El esquema de trabajo permite definir los requerimientos de adecuación de sitio, el plan de operaciones, el plan de monitoreo y establecer los tiempos de tratamiento. Esto resulta fundamental para poder definir la estructura de costos que sera la que en definitiva permita comparar la bio-remediación con la incineración

Biodegradação Ambiental , Incineração , Hidrocarbonetos