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1.
J Vet Diagn Invest ; 31(6): 879-882, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31585512

RESUMO

A large, ill-defined, firm, multinodular mass involving the pancreas was confirmed on postmortem examination of a 5-y-old, male Rottweiler that died following acute respiratory distress syndrome, after a period of anorexia and lethargy. Histologically, the mass consisted of plump spindle cells admixed with a variable number of macrophages, lymphocytes, plasma cells, and neutrophils. Foci of coagulative necrosis and hemorrhage were also observed. Spindle cells strongly reacted to antibodies against vimentin, α-smooth muscle actin, and calponin, whereas desmin was expressed only mildly and focally. Pan-cytokeratin, KIT, glial fibrillary acidic protein, and S100 protein were nonreactive. Variable numbers of MAC 387-positive cells, CD3+ lymphocytes, and numerous blood vessels were also detected throughout the mass. Histologic and IHC findings were consistent with a diagnosis of inflammatory myofibroblastic tumor of the pancreas.


Assuntos
Doenças do Cão/diagnóstico , Inflamação/veterinária , Neoplasias de Tecido Muscular/veterinária , Neoplasias Pancreáticas/veterinária , Animais , Doenças do Cão/patologia , Cães , Evolução Fatal , Inflamação/diagnóstico , Inflamação/patologia , Masculino , Neoplasias de Tecido Muscular/diagnóstico , Neoplasias de Tecido Muscular/patologia , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/patologia
2.
Vet Ital ; 55(3): 275-278, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31599553

RESUMO

An adult male Eurasian otter, found dead on the roadside, was submitted for post-mortem examination in April 2014 at the Veterinary Pathology Unit of the Faculty of Veterinary Medicine of Teramo, as part of the RECAL [RECovery and post mortem Analysis of Eurasian otters (Lutra lutra) in the National Park of Cilento, Vallo di Diano and Alburni (Salerno, Italy), and surrounding areas] project. Necropsy revealed an abundant hemothorax associated with multifocal, bilateral pulmonary contusions and lacerations, and a severe hemopericardium characterised by the presence of a wide blood clot in the intact pericardial sac. Two small laceration wounds of the left auricle were found at the base, along the atrioventricular groove, and on the outer free wall. Since myocardial and endocardial tissues showed no other gross and histopathological abnormalities, a left atrial appendage rupture resulting from a blunt chest trauma was diagnosed. Blunt traumatic cardiac rupture is a rarely reported, life-threatening condition in humans. To the best of our knowledge, this is the first report on a left atrial appendage rupture due to blunt chest trauma in veterinary literature. The possible occurrence of a cardiac rupture following a blunt thoracic injury should be taken into consideration in veterinary emergency care.


Assuntos
Apêndice Atrial/patologia , Traumatismos Cardíacos/veterinária , Lontras , Traumatismos Torácicos/veterinária , Ferimentos não Penetrantes/veterinária , Animais , Evolução Fatal , Traumatismos Cardíacos/diagnóstico , Traumatismos Cardíacos/patologia , Itália , Masculino , Traumatismos Torácicos/diagnóstico , Traumatismos Torácicos/patologia , Ferimentos não Penetrantes/diagnóstico , Ferimentos não Penetrantes/patologia
3.
Ecotoxicol Environ Saf ; 183: 109566, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31437728

RESUMO

Polybrominated diphenyl ethers (PBDEs) are brominated flame retardants. Biomonitoring studies have shown widespread presence of PBDEs in humans and their accumulation in food chain cause concern to human health, especially for foetus and infant development. The early-life stages are generally considered more sensitive to exposure to toxic compounds than juvenile or adults. For this reason the aim of this study was to evaluate the effects of the three most environmentally relevant BDE (BDE- 47, 99 and 209) on zebrafish embryos. The fish embryo toxicity (FET) OECD tests on zebrafish were performed followed by histopathogical examination to assess morphological changes. The gene expression of the thyroid stimulating hormone ß (Tshß), the transport proteins transthyretin (Ttr) and thyroxine-binding globulin (Tbg) as well as the enzyme iodothyronine deiodinase 1 (Dio1) was also assessed by Real-time PCR. BDE-47 and BDE-99 showed an increase of the severity of the effects at the lower concentrations while for the BDE-209 the effects were higher to the high concentrations. Although all compounds did not show any acute toxicity for none of the concentrations tested, they reported interesting sub-acute lesions, including yolk and pericardial edema, tail and head malformation, reduced and extremely reduced heart beat rate, blood stasis and spinal curvature, with the highest percentage recorded for BDE-209. Cardiac edema, damage of eye structure and hydrocephaly were confirmed also by histophatological examination. Furthermore, a toxic and dose-dependent liver vacuolization in BDE-209 was observed in all experimental groups. Although no statistically significant difference in gene expression was observed, BDE-209 up-regulated only Dio1 while the other congeners induced Tshß, Ttr, Tbg and Dio1. Overall, this research highlighted that exposure to BDE-47, BDE-99 and BDE-209 at realistic concentrations caused lethal and sub-lethal alterations and impaired genes involved in thyroid hormones homeostasis leading to abnormal development of zebrafish embryos.


Assuntos
Embrião não Mamífero/efeitos dos fármacos , Monitoramento Ambiental/métodos , Retardadores de Chama/toxicidade , Expressão Gênica/efeitos dos fármacos , Éteres Difenil Halogenados/toxicidade , Peixe-Zebra , Adulto , Animais , Relação Dose-Resposta a Droga , Embrião não Mamífero/patologia , Retardadores de Chama/análise , Éteres Difenil Halogenados/análise , Humanos , Hormônios Tireóideos/metabolismo , Peixe-Zebra/genética , Peixe-Zebra/metabolismo
4.
Vet Comp Oncol ; 17(3): 376-384, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31006970

RESUMO

Vasculogenic mimicry (VM) is an alternative type of blood perfusion characterized by formation of non-endothelial cell-lined microcirculatory channels and is responsible for aggressive tumour biology and increased tumour-related mortality. VM-correlated genes are associated with vascular endothelial grown factor receptor 1 (VEGFR1), and hypoxia-related (hypoxia inducible factor 1 α-HIF1α) signalling pathways, whose molecules are client proteins of Hsp90 (heat shock protein 90) and are potential therapeutic targets. This pilot study was aimed to investigate vasculogenic mimicry in a three-dimensional (3D) cell culture system of two aggressive canine osteosarcoma (OSA) cell lines (D22 and D17), and to evaluate the response of these cells to 17-AAG (17-N-allylamino-17-demethoxygeldanamycin) treatment in relation to tubular-like structure formation in vitro. Only D17 cell line formed hollow matrix channels in long-term 3D cultures and assumed endothelial morphology, with cells expressing both Hsp90 and VEGFR1, but lacking expression of endothelial marker CD31. 17-AAG treatment inhibited migration of D17 OSA cells, also decreasing VM markers in vitro and inducing a reduction of HIF1α transcript and protein in this cell line. Taken together, these preliminary data indicate that the biological effects of 17-AAG on D17 3D culture and on HIF1α regulation can provide interesting information to translate these findings from the basic research to clinical approach for the treatment of canine OSA as a model in comparative oncology.


Assuntos
Benzoquinonas/farmacologia , Técnicas de Cultura de Células/veterinária , Doenças do Cão , Neovascularização Patológica , Osteossarcoma/veterinária , Animais , Biomarcadores Tumorais , Técnicas de Cultura de Células/métodos , Linhagem Celular Tumoral , Cães , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/fisiologia , Proteínas de Choque Térmico HSP90/genética , Proteínas de Choque Térmico HSP90/metabolismo , Projetos Piloto , Molécula-1 de Adesão Celular Endotelial a Plaquetas/genética , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo
5.
Vet Sci ; 6(1)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30781786

RESUMO

Mast cells (MCs) are involved in angiogenesis, tissue remodeling and immunomodulation in several human and animal tumors, although their exact role is still controversial. Since no information is available in canine prostate carcinoma (PC) and normal prostate tissues, the aims of this study were to evaluate the possible correlations between MC distribution, molecular expression and microvessel density (MVD) in normal prostatic tissue and proliferative disorders of the canine prostate. All samples (6 normal, 15 benign prostate hyperplasia-BPH, 8 PC) were stained with Toluidine Blue and immunohistochemically evaluated for tryptase, c-Kit (CD117) and CD31. Mast cell density (MCD) and MVD were quantified by the hot-spot method. MCD was significantly increased in periglandular/peritumoral areas, when compared with intraglandular/intratumoral areas, in all groups (p = 0.03). C-Kit expression was strongly associated with PC (ρ = 0.75 p = 0.03), whereas positive correlation between tryptase and c-Kit expression (ρ = 0.64 p = 0.01) was observed in periglandular areas of BPH. MVD showed a correlation with MCD in BPH (ρ = 0.54 p = 0.04). Our data support the importance of c-Kit in regulating MC proliferation. The predominant location of MCs in peritumoral areas of canine PC was similar to the human counterpart, in which PC cells are supposed to produce substances attracting MCs to the tumor microenvironment.

6.
Vet Comp Oncol ; 17(3): 298-307, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30767361

RESUMO

Nectin-4 is an E-cadherin-based adherens junction protein of normal epithelial cells, as well as a potent mediator of anchorage-independent cancer colony formation. It is considered a tumour-associated histological and serological marker in various human cancers. The transcription factor p63 is a basal cell marker in the normal prostate, involved in cell adhesion, as well as in the formation and survival of circulating tumour cell clusters. The aim of this study was to evaluate Nectin-4 and p63 immunohistochemical expression in 42 canine prostate tissues including 2 normal prostates, 10 benign prostatic hyperplasias (BPHs), 30 prostatic carcinomas (PCs), 1 pulmonary and 1 lymph node metastasis. From normal to neoplastic tissues, Nectin-4 showed a progressive switching from membranous (m-Nectin-4) to cytoplasmic (c-Nectin-4), regardless of the histological subtypes, except for lack of expression in solid PCs. Metastatic cells exhibited both strong membranous and cytoplasmic positivity. c-Nectin-4 expression was significantly (P < 0.0001) increased in PCs/metastasis compared to BPHs cases and a decrease (P < 0.05) of nuclear p63 immunostaining was also detected in the two groups. Furthermore, data showed a significant association (P < 0.05) between p63 and m-Nectin-4 distribution, although their colocalization was detected only in scattered cells by double immunofluorescence. Our results suggest the involvement of m-Nectin-4 in canine prostate tumourigenesis and metastatic potential, while the exact role of c-Nectin-4 expression detectable in primary PCs requires further investigations.


Assuntos
Carcinogênese/metabolismo , Moléculas de Adesão Celular/metabolismo , Doenças do Cão/metabolismo , Regulação Neoplásica da Expressão Gênica/fisiologia , Neoplasias da Próstata/veterinária , Proteínas Supressoras de Tumor/metabolismo , Animais , Moléculas de Adesão Celular/genética , Cães , Masculino , Hiperplasia Prostática/metabolismo , Hiperplasia Prostática/veterinária , Neoplasias da Próstata/classificação , Neoplasias da Próstata/metabolismo , Proteínas Supressoras de Tumor/genética
7.
Parasitol Res ; 118(1): 43-46, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30315356

RESUMO

Despite the wide distribution of wild boar populations in Italy and the increase of its diffusion in urbanized areas, only one case report has described the occurrence of Echinococcus granulosus s.l. in a wild boar from Marche (Central Italy). The present study investigated the presence of E. granulosus sensu lato with an epidemiological survey on wild boars from central Italy that had been killed during hunting season. Seven hundred sixty-five (765) adult wild boars were examined during the 2016-2017 hunting season. Of these animals, 1.0% (8/765) were positive to E. granulosus s.l. with a fertility of 0.3% (2/765), and 2.9% animals (22/765) were positive for the metacestode stage of Taenia hydatigena (Cysticercus tenuicollis), while 0.5% (4/765) showed mixed infection (E. granulosus s.l. + T. hydatigena). Sixteen hydatids were found, of which 12.5% were fertile, 37.5% were sterile, 31.3% were caseous, and 18.8% were calcified. Eight hydatids (two fertile and six sterile cysts) were molecularly characterized by analysis of the mitochondrial gene, cytochrome c oxidase subunit 1 (cox1), and the NADH dehydrogenase subunit 1 (ND1). Hydatids found in wild boars were characterized as E. granulosus sensu stricto (G1 genotype). The present survey represents the first epidemiological study on cystic echinococcosis in wild boar in Italy which highlights the need for more extensive epidemiological investigations to determine the causal factors, economic impact, and public health importance of the disease in this livestock-wildlife setting.


Assuntos
Coinfecção/veterinária , Equinococose/veterinária , Echinococcus granulosus/isolamento & purificação , Sus scrofa/parasitologia , Doenças dos Suínos/epidemiologia , Animais , Coinfecção/epidemiologia , Coinfecção/parasitologia , Cistos , Equinococose/epidemiologia , Equinococose/parasitologia , Echinococcus granulosus/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genótipo , Itália/epidemiologia , Proteínas Mitocondriais/genética , Inquéritos e Questionários , Suínos , Doenças dos Suínos/parasitologia
8.
Arch Virol ; 164(1): 291-295, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30291502

RESUMO

The identification of avian poxvirus and avian papillomavirus associated with cutaneous lesions in griffon vultures (Gyps fulvus) by histopathology, electron microscopy and PCR analysis is reported. Sequence analysis of the fpv140 gene revealed 99% identity to two poxviruses obtained from a white-tailed sea eagle (Haliaeetus albicilla) and a common buzzard (Buteo buteo). Partial sequence of the papillomavirus L1 gene showed sequence similarity to papillomavirus LI genes from African grey parrot (Psittacus erithacus) (69% identity), duck (Anas platyrhynchos) (68% identity), and yellow-necked francolin (Francolinus leucoscepus) (66% identity). To date, this is the first identification of avian poxvirus and papillomavirus in griffon vultures and the first evidence of infection of both viruses in live wild birds.


Assuntos
Avipoxvirus/isolamento & purificação , Doenças das Aves/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/veterinária , Infecções por Poxviridae/veterinária , Animais , Avipoxvirus/genética , Doenças das Aves/epidemiologia , Falconiformes , Variação Genética , Itália/epidemiologia , Papillomaviridae/genética , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Filogenia , Infecções por Poxviridae/epidemiologia , Infecções por Poxviridae/virologia
10.
Res Vet Sci ; 105: 195-9, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27033932

RESUMO

Abnormalities in the retinoblastoma (Rb) and p53 tumour suppressor gene have been frequently detected in human and canine cancers, but never investigated in canine prostate cancer, considered a good model for the advanced and aggressive androgen-resistant prostate cancer in men. Therefore, the aim of this study was to evaluate the immunohistochemical expression of Rb and p53 in 6 normal canine prostates, 15 canine prostates with benign prostatic hyperplasia (BPH) and 10 prostatic carcinomas (PCs). In all normal samples, p53 was expressed in low number of epithelial cells, while a greater number of positive cells were observed in BPH and PC. The mean number of positive cells was statistically significantly higher in PCs than normal and hyperplastic prostates. A cytoplasmic or nucleo-cytoplasmic staining was observed in 5 out of 10 PCs. Rb protein was expressed in high number of normal, hyperplastic and neoplastic cells without a statistically significant differences. Considering that Rb is frequently lost in human prostate cancer, we suggest that Rb is not involved in canine prostatic carcinogenesis. On the other hand, the increased expression of p53 that corresponds to genetic defects in the p53 gene may be associated with the malignant growth of canine prostate cancer, conferring an apoptosis-resistant phenotype.


Assuntos
Doenças do Cão/genética , Hiperplasia Prostática/veterinária , Neoplasias da Próstata/veterinária , Proteína do Retinoblastoma/genética , Proteína Supressora de Tumor p53/genética , Animais , Carcinogênese , Doenças do Cão/fisiopatologia , Cães , Masculino , Hiperplasia Prostática/genética , Hiperplasia Prostática/fisiopatologia , Neoplasias da Próstata/genética , Neoplasias da Próstata/fisiopatologia , Proteína do Retinoblastoma/metabolismo , Proteína Supressora de Tumor p53/metabolismo
11.
Oxid Med Cell Longev ; 2015: 940131, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26078824

RESUMO

Aged dogs are considered the most suitable spontaneous animal model for studying normal aging and neurodegenerative diseases. Elderly canines naturally develop cognitive dysfunction and neuropathological hallmarks similar to those seen in humans, especially Alzheimer's disease-like pathology. Pet dogs also share similar living conditions and diets to humans. Oxidative damage accumulates in the canine brain during aging, making dogs a valid model for translational antioxidant treatment/prevention studies. Evidence suggests the presence of detective protein quality control systems, involving ubiquitin-proteasome system (UPS) and Heat Shock Proteins (HSPs), in the aged canine brain. Further studies on the canine model are needed to clarify the role of age-related changes in UPS activity and HSP expression in neurodegeneration in order to design novel treatment strategies, such as HSP-based therapies, aimed at improving chaperone defences against proteotoxic stress affecting brain during aging.


Assuntos
Envelhecimento , Encéfalo/metabolismo , Doenças Neurodegenerativas/patologia , Estresse Oxidativo , Animais , Modelos Animais de Doenças , Cães , Proteínas de Choque Térmico/metabolismo , Humanos , Doenças Neurodegenerativas/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Ubiquitina/metabolismo
12.
Vet Dermatol ; 26(4): 270-e59, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25962968

RESUMO

BACKGROUND: Recent investigations have highlighted the controversial role of Wnt/ß-catenin pathway activation in human cutaneous melanoma. Survivin has been proposed as a valid prognostic marker for invasive and metastatic melanomas and lymph node melanoma metastasis in human cutaneous melanoma and is a promising therapeutic target. HYPOTHESIS/OBJECTIVES: Our aim was to investigate the immunohistochemical expression of survivin and ß-catenin in canine cutaneous melanocytic tumours, in order to understand their prognostic significance. METHODS: Twenty-one melanocytic tumours (10 melanocytomas and 11 melanomas) were investigated by immunohistochemistry using specific anti-survivin and anti-ß-catenin antibodies. A semi-quantitative method was used to analyse the results; ß-catenin immunolabelling in neoplastic cells was evaluated as cytoplasmic, membranous or nuclear. The number of survivin-positive cells was counted within ~1000 neoplastic cells. Results were related to histopathological features, evaluated in haematoxylin- and eosin-stained slides, and to the clinical data obtained through a telephone survey with referring veterinarians. RESULTS: Despite a low level of expression in the majority of cases, ß-catenin was found to be correlated strongly with malignant behaviour (P < 0.01). An overexpression of nuclear survivin was statistically related to histological features of malignancy, presence of metastasis and death related to melanoma spread (P < 0.01). CONCLUSIONS AND CLINICAL IMPORTANCE: The low nuclear ß-catenin expression, mainly found in metastatic cases, would indicate that ß-catenin activation may have only limited importance in the development or progression of canine cutaneous melanoma. The correlation of nuclear survivin expression with malignancy would indicate that survivin is possibly a useful prognostic marker and therapeutic target in canine melanoma patients.


Assuntos
Doenças do Cão/patologia , Melanoma/veterinária , Proteínas de Neoplasias/metabolismo , Neoplasias Cutâneas/veterinária , beta Catenina/metabolismo , Animais , Cães , Feminino , Masculino , Melanoma/patologia , Metástase Neoplásica , Estudos Retrospectivos , Pele/patologia , Neoplasias Cutâneas/patologia
13.
Vet Ital ; 50(3): 199-202, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25273962

RESUMO

Adiaspiromycosis is a pulmonary disease caused by the inhalation of the ubiquitous fungus Emmonsia spp., a common soil inhabitant. Information about the replication and dissemination of the fungus from the primary site is lacking. Members of the Family Mustelidae seem to be highly susceptible to this infection, which has been previously reported in otters (Lutra lutra) in Czech Republic/Slovakia, Finland and in the UK. In many cases, Emmonsia­associated lesions have also been reported as incidental findings during necropsies of otherwise healthy animals. A road­killed male Eurasian otter was submitted for the post­mortem examination on 21st December 2009 at the Veterinary Pathology Unit of the Faculty of Veterinary Medicine of Teramo, as part of the RECAL [RECovery and post­mortem Analysis of Eurasian otters (Lutra lutra) in the National Park of Cilento, Vallo di Diano and Alburni (Salerno, Italy), and surrounding areas] project. Histologically, multifocal round structures with a PAS­positive thick tri­laminar wall and a central basophilic granular mass were observed within the alveoli. The adiaspores were surrounded by a severe granulomatous reaction with high number of macrophages, multinucleated giant cells, eosinophils, neutrophils and fibroblasts. Numerous multifocal cholesterol granulomas were observed close to those fungal­induced. To the best of our knowledge, this is the first description of adiaspiromycosis in an Eurasian otter in Italy.


Assuntos
Chrysosporium , Pneumopatias Fúngicas/veterinária , Lontras , Animais , Itália , Pneumopatias Fúngicas/patologia , Masculino
14.
Vet Dermatol ; 25(2): 138-41, e39-40, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24800266

RESUMO

BACKGROUND: Survivin has been identified as one of the most cancer-specific molecules, with a dual function of apoptosis inhibitor and orchestrator of cell division. HYPOTHESIS/OBJECTIVES: Based on our recent results obtained during the study of the role of survivin in epithelial-to-mesenchymal transition, we investigate its potential role in maintenance of stemness in both the normal canine hair follicle and related tumours. METHODS: We performed a simultaneous evaluation, by immunofluorescence, of the expression of survivin and CK15. CK15 was selected as a marker for epidermal and hair follicle stem cells, based on its ability to identify hair follicle stem cells in the normal hair follicle and in canine follicular tumours. In this study, six cases were selected from the cases of hair follicle tumours evaluated in previous studies, based on the highest immunoreactivity for survivin and CK15. Three samples of healthy canine skin were also included as a normal control. RESULTS: A partial co-localization of the molecules was observed in normal hair follicles, as well as in trichoepitheliomas and trichoblastomas. In particular, a different co-expression was observed in relationship to the hair follicle cycle stage. CONCLUSIONS AND CLINICAL IMPORTANCE: These findings suggest that survivin could play an important role in the maintenance of the hair follicle cycle as well as in tumour initiation and maintenance of cancer stem cells.


Assuntos
Inibidores de Cisteína Proteinase/metabolismo , Doenças do Cão/metabolismo , Folículo Piloso/patologia , Proteínas Inibidoras de Apoptose/metabolismo , Neoplasias Cutâneas/veterinária , Células-Tronco/metabolismo , Animais , Biomarcadores/sangue , Inibidores de Cisteína Proteinase/sangue , Doenças do Cão/sangue , Doenças do Cão/diagnóstico , Cães , Transição Epitelial-Mesenquimal/fisiologia , Regulação Neoplásica da Expressão Gênica , Proteínas Inibidoras de Apoptose/sangue , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/metabolismo
15.
Vet Parasitol ; 201(1-2): 158-62, 2014 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-24548702

RESUMO

Aelurostrongylus abstrusus affects the respiratory system of domestic cats worldwide, causing different clinical signs. Recently, Troglostrongylus brevior, a parasite found only in feral and wild animals until 2010, has been regarded as a cause of potentially severe bronchopneumonia in domestic cats. The life cycle of both nematodes involves the same intermediate (i.e. land mollusks) and paratenic hosts (i.e. lizards, birds and small mammals). Biological and epidemiological drivers may cause the spreading of A. abstrusus along with a possible emergence of T. brevior from marginal areas and limited hosts. Given the merit in increasing knowledge on these potentially emerging nematodes, the present paper describes clinical features and the first post mortem gross and histopathological observations in a case of mixed infection by A. abstrusus and T. brevior in a kitten.


Assuntos
Doenças do Gato/patologia , Metastrongyloidea/fisiologia , Infecções por Strongylida/veterinária , Animais , Doenças do Gato/parasitologia , Gatos , Coinfecção , Evolução Fatal , Feminino , Infecções por Strongylida/parasitologia , Infecções por Strongylida/patologia
16.
Res Vet Sci ; 96(1): 143-6, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24374119

RESUMO

This report describes the morphological and immunohistochemical features of intracytoplasmic inclusion bodies found in a 13-year-old Yorkshire dog with a hepatocellular carcinoma and referred for anorexia, lethargy and mild polydipsia. Fine-needle aspirates of the large abdominal mass revealed high number of pleomorphic neoplastic hepatocytes, containing round to polygonal, well-demarcated, hyaline bodies. Same findings were histologically confirmed on multiple biopsies. Immunohistochemically, the inclusion bodies were negative for alpha-1-antitrypsin, carcinoembryonary antigen, fibrinogen, IgG, IgM, cytokeratins 7, 8, 18, 19, 20. By transmission electron microscopy, the cytoplasmic inclusions were composed of granular homogeneous or reticulated electrondense matrix, enclosed within dilated rough endoplasmic reticulum or remnants of its membranes, consistent with proteinaceous material accumulated within neoplastic hepatocytes due to aberrant protein secretion or transport. This is the first detailed characterization of hyaline cytoplasmic inclusion bodies in canine hepatocellular carcinoma.


Assuntos
Carcinoma Hepatocelular/veterinária , Doenças do Cão/patologia , Corpos de Inclusão/patologia , Neoplasias Hepáticas/veterinária , Animais , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Doenças do Cão/diagnóstico por imagem , Cães , Evolução Fatal , Hialina/diagnóstico por imagem , Imuno-Histoquímica/veterinária , Corpos de Inclusão/diagnóstico por imagem , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Masculino , Microscopia Eletrônica de Transmissão/veterinária , Ultrassonografia
17.
J Zoo Wildl Med ; 45(4): 973-8, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25632696

RESUMO

Two boa constrictors (Boa constrictor imperator) presented with paresis of the trunk originating cranial to the cloaca. Radiographs were consistent with proliferative bone lesions involving several vertebrae. Computed tomography (CT) demonstrated the presence of lytic/expansile lesions. Computed tomography-guided biopsies of the lesions were performed without complications. Histology was consistent with bacterial osteomyelitis and osteoarthritis. Gram-negative bacteria (Salmonella sp. and Pseudomonas sp.) were isolated from cultures of the biopsies. Medical treatment with specific antibiotics was attempted for several weeks in both cases without clinical or radiographic improvements. The animals were euthanized, and necropsy confirmed the findings observed upon CT. To the authors' knowledge, this is the first report of the use of CT-guided biopsies to evaluate proliferative vertebral lesions in snakes. In the present report, CT-guided biopsies were easily performed, and both histologic and microbiologic results were consistent with the final diagnosis.


Assuntos
Boidae , Osteomielite/veterinária , Coluna Vertebral/patologia , Tomografia Computadorizada por Raios X/veterinária , Animais , Antibacterianos/uso terapêutico , Biópsia/métodos , Biópsia/veterinária , Feminino , Masculino , Osteomielite/diagnóstico , Osteomielite/tratamento farmacológico , Osteomielite/microbiologia , Osteomielite/patologia , Infecções por Pseudomonas/tratamento farmacológico , Infecções por Pseudomonas/microbiologia , Infecções por Pseudomonas/patologia , Infecções por Pseudomonas/veterinária , Pseudomonas aeruginosa/isolamento & purificação , Salmonelose Animal/tratamento farmacológico , Salmonelose Animal/microbiologia , Salmonelose Animal/patologia , Coluna Vertebral/microbiologia
18.
ScientificWorldJournal ; 2013: 401468, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24288481

RESUMO

The Mexican Goodeid, Zoogoneticus tequila, is considered nearly extinct in the wild and it is maintained in captivity by the nonprofit international "Goodeid Working Group." The unique Italian colony has produced about 180 fish so far. The observable diseases were registered and some fish were submitted, immediately after spontaneous death, to necroscopic and histopathologic exams. Encountered diseases included the following: 7 cases of scoliosis (2 males and 5 females); 2 fish with a similar congenital deviation of ocular axis; 1 adult male with left corneal opacity, presumably of traumatic origin; 1 female fish with a large subocular fluid-filled sac, histologically referable to a lymphatic cyst, similarly to the eye sacs of a Goldfish variety (Carassius auratus) called bubble eye; and 1 female fish with recurrent abdominal distension consequent to distal bowel dilation and thinning, associated with complete mucosal atrophy, and comparable to intestinal pseudo-obstruction syndromes described in humans and various animal species. The absence of infectious or parasitic diseases, as well as the low incidence of diseases potentially related to environmental alterations or nutritional disorders such as spinal deformities, suggests the adequacy of breeding management techniques of Z. tequila for its conservation and reintroduction in to the original habitat in the near future.


Assuntos
Ciprinodontiformes/anormalidades , Doenças dos Peixes/patologia , Animais , Cruzamento , Ciprinodontiformes/lesões , Olho/patologia , Feminino , Intestinos/patologia , Masculino , Escoliose/veterinária
19.
Asian Pac J Trop Biomed ; 3(3): 186-90, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23620835

RESUMO

OBJECTIVE: To describe the occurrence of various spinal deformations in a captive-bred wild line of Poecilia wingei (P. wingei). METHODS: Fish belonging to a wild line of P. wingei caught from Laguna de Los Patos, Venezuela, were bred in an aquarium home-breeding system during a period of three years (2006-2009). The spinal curvature was observed to study spinal deformities in P. wingei. RESULTS: Out of a total of 600 fish, 22 showed different types of deformities (scoliosis, lordosis, kyphosis), with a higher incidence in females. Growth, swimming and breeding of deformed fish were generally normal. CONCLUSIONS: Possible causes for spinal curvature in fish are discussed on the basis of the current literature. While it is not possible to determine the exact cause(s) of spinal deformities observed in the present study, traumatic injuries, nutritional imbalances, genetic defects or a combination of these factors can be supposed to be involved in the pathogenesis of such lesions.


Assuntos
Cifose/veterinária , Lordose/veterinária , Poecilia/anormalidades , Escoliose/veterinária , Animais , Feminino , Incidência , Cifose/congênito , Cifose/epidemiologia , Lordose/congênito , Lordose/epidemiologia , Masculino , Poecilia/crescimento & desenvolvimento , Poecilia/fisiologia , Reprodução , Escoliose/congênito , Escoliose/epidemiologia , Natação
20.
J Cell Biochem ; 114(1): 134-43, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22886939

RESUMO

Mesenchymal stem cells (MSCs) are an important cell population in the bone marrow microenvironment. MSCs have the capacity to differentiate in vitro into several mesenchymal tissues including bone, cartilage, fat, tendon, muscle, and marrow stroma. This study was designed to isolate, expand, and characterize the differentiation ability of sheep bone marrow-derived MSCs and to demonstrate the possibility to permanently express a reporter gene. Bone marrow was collected from the iliac crest and mononuclear cells were separated by density gradient centrifugation. Sheep MSCs cell lines were stable characterized as CD44+ and CD34- and then transfected with a green fluorescent protein (GFP) reporter gene. The GFP expression was maintained in about half (46.6%) of cloned blastocysts produced by nuclear transfer of GFP+ sheep MSCs, suggesting the possibility to establish multipotent embryonic cells' lines carrying the fluorescent tag for comparative studies on the differentiation capacity of adult stem cells (MSCs) versus embryonic stem cells. We found that sheep MSCs under appropriate culture conditions could be induced to differentiate into adipocytes, chondrocytes, and osteoblast lineages. Our results confirm the plasticity of sheep MSCs and establish the foundation for the development of a pre-clinical sheep model to test the efficiency and safety of cell replacement therapy.


Assuntos
Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Adipócitos/citologia , Adipócitos/fisiologia , Animais , Antígenos CD34/genética , Blastocisto/citologia , Blastocisto/fisiologia , Células da Medula Óssea/fisiologia , Diferenciação Celular , Linhagem da Célula , Centrifugação com Gradiente de Concentração , Condrócitos/citologia , Condrócitos/fisiologia , Feminino , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Receptores de Hialuronatos/genética , Células-Tronco Mesenquimais/fisiologia , Técnicas de Transferência Nuclear , Oócitos/citologia , Oócitos/fisiologia , Osteoblastos/citologia , Osteoblastos/fisiologia , Ovinos , Carneiro Doméstico , Coloração e Rotulagem/métodos
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