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1.
Artigo em Inglês | MEDLINE | ID: mdl-33389075

RESUMO

PURPOSE: Approximately 60% of patients with melanoma harbor BRAF mutation and targeting BRAF offers enormous advance in the treatment of those patients. Unfortunately, the efficacy of the BRAF inhibitors is usually restricted by the onset of drug resistance. Therefore, better understanding of the adaptive drug resistance mechanisms is essential for the development of alternative therapeutic strategies, and offers more promising measures to promote the short duration of response to BRAF inhibitors. METHODS: The levels of tumor suppressive long noncoding RNA on chromosome 8p12 (TSLNC8) were evaluated by qPCR. The MTT assay, colony formation assay, apoptosis assay, and in vivo xenograft tumor model were performed to assess the functions of TSLNC8 on drug resistance. Western blotting, RNA pull-down, and RNA immunoprecipitation (RIP) assays were applied to investigate the mechanisms of TSLNC8 in melanoma. RESULTS: Herein, our findings demonstrate that TSLNC8 is significantly downregulated in BRAF inhibitor-resistant melanoma tissues and cells. Moreover, downregulation of TSLNC8 in BRAF inhibitor sensitive cells reduces the toxicity response to BRAF inhibitor PLX4720, and inhibits apoptosis of melanoma cells-treated with PLX4720. Further assay elucidates that TSLNC8 can bind with the catalytic subunit of protein phosphatase 1α (PP1α) to regulate its distribution, and Downregulation of TSLNC8 results in PP1α cytoplasmic accumulation, thus re-activating the MAPK signaling. Eventually, the overexpression of TSLNC8 in BRAF inhibitor PLX4720-resistant melanoma cells restores the sensitive to BRAF inhibitor. CONCLUSION: Collectively, our research provides a compelling rationale for resistance to BRAF inhibitor in melanoma, and the patient might benefit from the combinatorial therapy of BRAF inhibitors and lncRNA TSLNC8.

2.
Life Sci ; 268: 118925, 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33358903

RESUMO

AIMS: Osteosarcoma (OS) is an extremely malignant bone cancer with high incidence and rapid progression. This study aims to investigate the role and underlying mechanisms of MALAT1 and miR-485-3p in OS. MATERIALS AND METHODS: qRT-PCR and Western blotting were utilized to measure the levels of miR-485-3p, MALAT1, c-MET, AKT3, p-mTOR, mTOR, glycolysis-related proteins or migration-related proteins. Colony formation and transwell assay were used to test the roles of miR-485-3p, MALAT1, c-MET and AKT3 in cancer cell proliferation, migration and invasion. Dual luciferase assay was used to validate the interactions of miR-485-3p/c-MET, miR-485-3p/AKT3, and MALAT1/miR-485-3p. Glucose uptake assay and measurement of lactate production were employed to determine the glycolysis process. Mouse tumour xenograft model was used to determine the effect of shMALAT1 and miR-485-3p mimics on tumour growth and metastasis in vivo. KEY FINDINGS: miR-485-3p was decreased while c-MET, AKT3, and MALAT1 were increased in human OS tissues and cells. miR-485-3p bound directly to c-MET and AKT3 mRNAs and repressed OS cell glycolysis, proliferation, migration, and invasion through decreasing glycolysis-related proteins and migration-related proteins via inhibiting c-MET and AKT3/mTOR pathway. In addition, MALAT1 interacted with miR-485-3p and disinhibited c-MET and AKT3/mTOR signalling. Knockdown MALAT1 or overexpression of miR-485-3p restrained OS tumour growth and lung metastasis in vivo. SIGNIFICANCE: miR-485-3p suppresses OS glycolysis, proliferation, and metastasis via inhibiting c-MET and AKT3/mTOR signalling and MALAT1 acts as a sponge of miR-485-3p. MALAT1 and miR-485-3p may be the key regulators in OS progression, and potential molecular targets for future OS therapy.

3.
Bioeng Transl Med ; : e10202, 2020 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-33349797

RESUMO

The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein contains an immunogenic receptor-binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S-RBD vaccine using a dissolvable microneedle skin patch can induce both significant B-cell and significant T-cell responses against S-RBD. Importantly, the outcomes were comparable to that of conventional bolus injection.

4.
Front Cell Dev Biol ; 8: 528155, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33195183

RESUMO

Prostate cancer is the most common malignant tumor of the urinary system. The mechanisms of the initiation and progression of prostate cancer have not been fully elucidated. Increasing evidence suggests that circular RNAs (circRNAs) are involved in cancer pathogenesis. In this study, we aimed to identify differentially expressed circRNAs in prostate cancer tissues and explored the role of circRNAs in the pathogenesis of prostate cancer. By screening a circRNA microarray assay, we found that circ_0088233 was upregulated in prostate cancer tissues compared to adjacent normal tissues, and this upregulation can be verified in 46 pairs of prostate cancer and adjacent normal tissues examined using quantitative reverse transcription-PCR. The level of circ_0088233 correlated with the TNM stage. Knockdown of circ_0088233 reduced cell proliferation, migration, invasion, and induced G1 phase arrest and apoptosis. In addition, miR-185-3p was identified as the downstream target of circ_0088233 using luciferase reporter assays and a biotinylated circ_0088233 probe pull-down assay. The miR-185-3p level showed a negative correlation with the circ_0088233 level in prostate cancer tissues. Overexpression of circ_0088233 blocked the effects of miR-185-3p on cell proliferation, migration, invasion, cell cycle, and apoptosis. In conclusion, circ_0088233 may function as an oncogene and play an oncogenic role by sponging hsa-miR-185-3p. This study increases the understanding of circRNAs in the progression of prostate cancer. These results implicate circ_0088233 as a potential therapeutic target for prostate cancer.

5.
Langmuir ; 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33147955

RESUMO

Liquid-liquid dispersion coupled with droplet formation and mass transfer of surfactants is one of the most typical phenomena in many chemical processes. As in every aspect of this process, the interfacial tension variation caused by the unsaturated adsorption of surfactants on the droplet surface plays an important role. This article focuses on microdroplet formation and the dynamic interfacial behavior of surfactants in the jetting regime. In a capillary embedded step T-junction device, controllable preparation of monodisperse droplets is achieved, and a correlation for predicting droplet sizes is established. A method for measuring the dynamic interfacial tension is provided. Mass transfer coefficients are then calculated for Tween 20 during the droplet formation process by a semiempirical correlation. The results indicate that dynamic interfacial tensions are lower than those obtained when the surfactant is adsorbed to equilibrium. Based on the tip-streaming phenomenon, mass transfer coefficients for Tween 20 can reach up to ∼10-3 m/s, higher than those obtained in coaxial microfluidic devices. All the preliminary results shed light on the nature of droplet formation and will be of significance for application in industrial apparatuses.

6.
ACS Nano ; 14(11): 14715-14730, 2020 11 24.
Artigo em Inglês | MEDLINE | ID: mdl-33156626

RESUMO

Ferroptotic antitumor therapy has been compromised by various intracellular antioxidants, particularly glutathione and thioredoxin. Both are cofactors of glutathione peroxide 4 (GPX4) that act against oxidative stress via catalyzing the reduction of lipid peroxides. It was postulated that tailored polymer micelles could enhance ferroptotic antitumor efficacy via diminishing glutathione and thioredoxin under hypoxia. The aim was to engineer hypoxia-responsive micelles for selective enhancement of ferroptotic cell death in solid tumor. The polymer contains hydrophilic poly(ethylene glycol) (PEG) that is linked by azobenzene linker with nitroimidazole-conjugated polypeptide. The tailored polymer could self-assemble into nanoscale micelles to encapsulate RAS-selective lethal small molecule 3, a covalent GPX4 inhibitor. Under hypoxia, the azobenzene moiety enabled PEG shedding and enhanced micelles uptake in 4T1 cells. Likewise, the nitroimidazole moiety was reduced by the overexpressed nitroreductase with reduced nicotinamide adenine dinucleotide phosphate (NADPH) as the cofactor, resulting in transient depletion of NADPH. This impaired both the glutathione and thioredoxin redox cycle, leading to diminished intracellular glutathione and thioredoxin. The selective potency of ferroptotic micelles in depleting NADPH, glutathione and thioredoxin was further verified in vivo in the 4T1 tumor xenograft mice model. This work highlights the role of hypoxia-responsive polymers in enhancing the potency of ferroptotic inducers against solid tumors without additional side effects to healthy organs.

7.
Medicine (Baltimore) ; 99(40): e22620, 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33019484

RESUMO

RATIONALE: Low-dose mitotane has been widely used for many decades in patients with advanced adrenocortical carcinoma (ACC), which exhibited good safety profiles compared with the high-dose regimen. The clinical efficacy and toxicity of mitotane are closely related to its plasma concentration, and therapeutic drug monitoring (TDM) is recommended. Until now, no severe adverse drug reaction (ADR) related to the toxic plasma level after a short-term treatment of low-dose mitotane has been published. PATIENT CONCERNS: A 50-year-old Chinese female presented with severe neurological adverse events related to a toxic plasma levels of 42.8 mg/L after 4 months treatment of low-dose mitotane. DIAGNOSES: During the course of therapy, no other medication could cause neurological adverse events. Therefore, we suspected a high sensitivity to the side effect of mitotane related to a toxic plasma level. INTERVENTIONS: Treatment of mitotane was stopped. OUTCOMES: The trough plasma concentration of mitotane decreased to 18.7 mg/mL after one and a half months, and the neurological symptoms gradually improved after drug discontinuance. LESSONS: The present case provides the first report of severe neurological adverse events induced by the short-term use of low-dose mitotane for adjuvant treatment in a patient with ACC, indicating that potentially severe ADR can also occur when using low-dose regimen in the early stage of treatment. TDM and early recognition could result in a favorable outcome.


Assuntos
Neoplasias do Córtex Suprarrenal/patologia , Carcinoma Adrenocortical/tratamento farmacológico , Antineoplásicos Hormonais/toxicidade , Mitotano/toxicidade , Doenças do Sistema Nervoso/induzido quimicamente , Antineoplásicos Hormonais/sangue , Antineoplásicos Hormonais/uso terapêutico , Grupo com Ancestrais do Continente Asiático/etnologia , Monitoramento de Medicamentos , Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos , Feminino , Humanos , Pessoa de Meia-Idade , Mitotano/sangue , Mitotano/uso terapêutico , Síndromes Neurotóxicas , Resultado do Tratamento , Suspensão de Tratamento
8.
Chem Commun (Camb) ; 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33124644

RESUMO

We obtained two high-quality lamellar crystals of cayno-substituted p-phenylene vinylene derivatives and fabricated their transistors. The transistors demonstrated hysteresis-free, low subthreshold swing values of 0.15 and 0.10 V dec-1, and high mobilities of 0.56 and 2.73 cm2 V-1 s-1, respectively. More importantly, the low defect density of 1 per 9.22 × 103 and 1.82 × 104 molecules, respectively, confirmed their high crystal quality.

9.
Huan Jing Ke Xue ; 41(9): 4042-4052, 2020 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124284

RESUMO

Eutrophication of shallow lakes in the middle and lower reaches of the Yangtze River has become an increasingly serious problem. In this study, we investigated the temporal and spatial variations in nutrient loads (nitrogen, N and phosphorus, P) in the Changdang Lake Catchment located to the northwest of Lake Taihu through field sampling and laboratory analysis in 2016-2017. The results show the severity of the N and P pollution in the Changdang Lake catchment. The mean river water concentrations of TN, NO3--N, NH4+-N, TP, Chla, and permanganate index are (3.70±0.76) mg ·L-1, (1.81±0.42) mg ·L-1, (1.03±0.61) mg ·L-1, (0.38±0.31) mg ·L-1, (25.74±37.00) µg ·L-1, and (6.35±0.81) mg ·L-1, respectively. N pollution in the river is more severe in winter and spring than in summer and autumn whereas P pollution in the river is worse in autumn and winter than in spring and summer. Spatially, the magnitude of river N and P pollution follows the order of northern > northwestern > southern > eastern part of the study area. The rivers are in a state of moderate to severe eutrophication. The mean lake water concentrations of TN, NO3--N, NH4+-N, TP, Chla, and permanganate index are (2.25±0.94) mg ·L-1, (0.98±0.47) mg ·L-1, (0.19±0.14) mg ·L-1, (0.11±0.03) mg ·L-1, (18.71±8.76) µg ·L-1, and (4.59±1.09) mg ·L-1, respectively. The water quality in Changdang Lake is categorized as worse than class Ⅲ for TN and TP concentrations, which show decreasing trends from the west to the east to the south of the lake. The lake is in a status of slight to moderate eutrophication. The lake water quality is affected by the combination of sewage discharge and non-point source pollutant losses. The inflow rivers including the Danjinlicao River, Tongji River, and Xuebu River are the dominant pollution sources for Changdang Lake. The Danjinlicao River transports 10-12 times the total N and P loads transported by Tongji and Xuebu rivers. Changes in land use and atmospheric deposition are the driving factors of the deterioration of water quality and eutrophication in the catchment.


Assuntos
Rios , Poluentes Químicos da Água , China , Monitoramento Ambiental , Eutrofização , Lagos , Nitrogênio/análise , Nutrientes , Fósforo/análise , Poluentes Químicos da Água/análise
10.
ACS Omega ; 5(37): 23736-23742, 2020 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-32984692

RESUMO

The selectivity of a chemical reaction is related to the effective utilization of raw materials as well as the cleanliness and economy of the process. Herein, it has been first proposed to synthesize tetraethyl thiuram disulfide (TETD) with sodium bicarbonate as the pH regulator with a reaction selectivity of ∼100%. The existence of a reaction intermediate, a sodium salt of diethyl dithiocarbamoylsulfenic acid (NaEt2DTCS), has been proved by experiments and theoretical calculations. The results indicate that TETD can not only be generated from NaEt2DTCS oxidized by H2O2 directly, but also from the conjugation of NaEt2DTC and NaEt2DTCS generated in the first stage of oxidation meanwhile. Accordingly, an oxidation reaction pathway has been proposed. The reaction selectivity with NaHCO3 or CO2 as the pH regulator has been compared, and the selectivity control mechanism is discussed. At relatively higher pH values with NaHCO3 as the pH regulator, peroxidation could be almost avoided.

11.
Transl Pediatr ; 9(4): 551-560, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32953553

RESUMO

Background: Chylothorax is a rare disease, defined as an abnormal accumulation of chylous lymphatic effusion in thoracic cavity, with a high mortality rate in pediatric patients. At present, there are few studies on the treatment of pediatric chylothorax, and conservative treatments like somatostatin (SST) and pleurodesis are performed empirically. SST has been used for treating pediatric chylothorax over 20 years, and povidone-iodine chemical pleurodesis (PICP) is adopted in recent years with a high cure rate, but both the effect are still uncertain. The safety and efficacy of SST and PICP in treating pediatric chylothorax was compared in this study. Methods: From January 2009 to May 2020, 27 pediatric chylothorax patients who accepted life support, SST and PICP treatment were retrospectively reviewed in this study. Their medical history, clinical manifestations, pleural effusion examinations, treatment methods, effects, and complications were screened. PICP and SST was performed in 19 and 8 children, respectively. Each patient was followed up after discharge from the hospital. Results: After admission, chest X-ray and ultrasound diagnosed pleural effusion in 27 patients (18 males and 9 females), including 13 and 6 cases on the left and right side, and 8 cases on both sides. In the pleural effusion, the mean leukocyte count was (9,826±9,482)×106/L, the mean lymphocyte ratio was (84.82±6.58)%, the mean triglyceride content was 7.11±6.63 mmol/L. In PICP and SST group, the mean length of stay was 38.42±19.42 and 35.13±12.72 d (P=0.664), the mean time of thoracic drainage was 24.58±16.34 and 19.63±8.88 d (P=0.440), the mean time of parenteral nutrition was 27.16±18.29 and 25.25±13.52 d (P=0.793), respectively. PICP was performed for 3.16±2.27 times, and SST was used for 14.75±9.08 d with the dosage of 0.5 to 10 µg/kg/hour. After the treatment, all patients in PICP group had mild chest pain and low-grade fever, and 12 cases were observed with transient decrease of oxygen saturation. Eight patients in SST group had nausea and vomit, and 1 child had diarrhea simultaneously. Both were cured by symptomatic treatment, thoracic drainage and discontinuing using SST. Three children were diagnosed with refractory chylothorax and cured by PICP after SST. Pleural effusion in 27 patients was finally absorbed. All of them achieved a clinical cure successfully, and there was no recurrence during discharge follow-up. Conclusions: PICP has a high success rate and few adverse reactions in treating spontaneous chylothorax in pediatric patients, expected to be a new treatment option for pediatric chylothorax after SST.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32885944

RESUMO

A cyano-substituted styrene derivative is synthesized and successfully prepared to lamellate single crystals through precisely controlling the crystal growth conditions. The lamellate single crystals with regular edge and smooth surface display intrinsically ordered stacking and high quality, all of which are of importance for high optoelectronic performance. The single-component light-emitting transistors based on the lamellate crystals offer striking device performance in terms of record external quantum efficiency of 2.02%, exceeding the benchmark value in this field. Such organic light-emitting single crystals provide a versatile platform for designing and engineering their structures and optoelectronic properties toward light-emitting devices.

13.
J Zhejiang Univ Sci B ; 21(9): 749-751, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32893532

RESUMO

Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was found initially in Wuhan, China in early December 2019. The pandemic has spread to 216 countries and regions, infecting more than 23310 000 people and causing over 800 000 deaths globally by Aug. 24, 2020, according to World Health Organization (https://www.who.int/emergencies/diseases/ novel-coronavirus-2019). Fever, cough, and dyspnea are the three common symptoms of the condition, whereas the conventional transmission route for SARS-CoV-2 is through droplets entering the respiratory tract. To date, infection control measures for COVID-19 have been focusing on the involvement of the respiratory system. However, ignoring potential faecal transmission and the gastrointestinal involvement of SARS-CoV-2 may result in mistakes in attempts to control the pandemic.


Assuntos
Betacoronavirus/isolamento & purificação , Infecções por Coronavirus/transmissão , Infecções por Coronavirus/virologia , Fezes/virologia , Gastroenteropatias/virologia , Pneumonia Viral/transmissão , Pneumonia Viral/virologia , Betacoronavirus/genética , China/epidemiologia , Infecções por Coronavirus/epidemiologia , Microbiologia Ambiental , Humanos , Modelos Biológicos , Pandemias , Pneumonia Viral/epidemiologia , RNA Viral/análise , RNA Viral/genética , Eliminação de Partículas Virais
14.
J Clin Lab Anal ; 34(11): e23469, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32822099

RESUMO

OBJECTIVE: To investigate the role of COLEC12 in osteosarcoma and observe the relationship between COLEC12 knockdown and the inflammation of osteosarcoma. Then, further explore whether the process is regulated by TLR4. METHOD: GEPIA and TCGA systems were used to predict the potential function of COLEC12. Western blot and RT-PCR were used to analyze the protein expression, or mRNA level, of COLEC12 in different tissue or cell lines. The occurrence and development of osteosarcoma were observed by using COLEC12 knockdown lentivirus. The inflammation indexes of osteosarcoma, in vitro and in vivo, were explored. TLR4 knockdown lentivirus was applied to the relationship between COLEC12 and TLR4. RESULTS: COLEC12 expression in SARC tumor tissue was higher than in normal, and a high expression of COLEC12 in SARC patients had a worse prognostic outcome. Pairwise gene correlation analysis revealed a potential relationship between COLEC12 and TLR4. The COLEC12 expression and mRNA level in the tumor or Saos-2 cells were increased. COLEC12 knockdown lentivirus could inhibit osteosarcoma development, in vivo and vitro, through reducing tumor volume and weight, weakening tumor proliferation, migration, and invasion, and enhancing apoptosis. Furthermore, COLEC12 knockdown could increase inflammation of osteosarcoma, in vivo and in vitro, through inducing myeloperoxidase (MPO), TLR4, NF-κB, and C3, and expression of related inflammatory factors. Finally, TLR4 knockdown lentivirus inhibits the progress of inflammation after COLEC12 regulation, in vivo and vitro. CONCLUSION: COLEC12 may be able to regulate apoptosis and inflammation of osteosarcoma, and TLR4 may be the downstream target factor of COLEC12 in inflammation.

15.
Antimicrob Resist Infect Control ; 9(1): 133, 2020 08 14.
Artigo em Inglês | MEDLINE | ID: mdl-32799915

RESUMO

BACKGROUND: There are no current national estimates of the candidaemia burden in China, and epidemiological candidaemia data from the underdeveloped region of China are lacking. METHODS: A 7-year retrospective study was carried out to analyse the prevalence, species distribution, antifungal susceptibility, risk factors and inpatient mortality of candidaemia among paediatric and adult patients in a regional tertiary teaching hospital in China. RESULTS: During the seven-year study period, a total of 201 inpatients with candidaemia were identified. The median age of the patients was 65 years (range, 1 day to 92 years), and 114 of the patients (56.7%) were male. The mean annual incidence of candidaemia was 0.26 cases per 1000 admissions (0.42 cases per 1000 paediatric admissions vs 0.24 cases per 1000 adult admissions, P < 0.05). Candida albicans was the most common fungal species (81/201, 40.3%) in all patients, Candida glabrata was the most common fungal species (18/35, 51.4%) in paediatric patients. Most isolates were susceptible to flucytosine (99.0%) and amphotericin B (99.0%), and the activity of antifungal agents against Candida species was no significant difference in satisfaction between paediatric and adult patients (P > 0.05). The all-cause mortality rate was 20.4% (paediatric patients: 11.4% vs adult patients:22.3%, P > 0.05). Fewer univariate predictors of poor outcomes were identified for paediatric patients than for adult patients (4 vs 11 predictors). Respiratory dysfunction and septic shock were independent predictors of 30-day mortality for all patients. CONCLUSIONS: The epidemiological data of candidaemia in paediatric and adult patients are only different in the distributions of Candida species and the mean annual incidence of candidaemia. Flucytosine and amphotericin B can be used as first-choice agents when no antifungal susceptibility test results are available.

17.
Cancer Manag Res ; 12: 6947-6958, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801915

RESUMO

Aim: To investigate the function of Aurora kinase B (AURKB) in gastric cancer (GC). Methods: Immunohistochemistry was used to assay the expression of AURKB in 50 pairs of GC and adjacent tissues, and qRT-PCR was conducted to test AURKB expression in normal gastric epithelial and GC cell lines. Two segments of small interference RNAs (siRNAs) targeting AURKB were synthesized and inserted into GV248 lentivirus vector. After transfected with LV-AURKB-RNAis, CCK8, wound healing, transwell and flow cytometric assays were performed to determine the influence of silencing AURKB on cell proliferation, invasion, migration, cell cycles and apoptosis of GC cells, and the expression of EMT (epithelial-mesenchymal transition)-related markers was demonstrated by Western blots (WB). Results: AURKB was highly expressed in GC and closely associated with lymph node metastasis and advanced stages of GC. Down-regulating AURKB suppressed the proliferation and promoted the apoptosis of GC cells, arrested the cell cycle in G2/M phase, and inhibited the invasion and migration of GC cells. The expression levels of AKT1, mTOR, Myc, MMP2, and VEGFA were decreased, while the expression levels of OCLN and JUP were increased after knocking down of AURKB in both AGC and MKN45 cells. Conclusion: AURKB is overexpressed in GC and closely associated with clinicopathologic characteristics of GC. It is likely that by inhibiting VEGFA/Akt/mTOR and Wnt/ß-catenin/Myc pathways, silenced AURKB could inhibit the invasive and migratory abilities of GC cells. However, because of the small sample size and the absence of in-vivo experiments, these results should be verified by further studies.

19.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(4): 488-493, 2020 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-32691555

RESUMO

Objective: To investigate the effect of phosphatidyl inositol 3-kinase/protein kinase B (PI3K/AKt) signaling pathway on the apoptosis of alveolar macrophages (AM) induced by nano-silica (NS) dust. Methods: After exposure to different concentrations of NS suspension, CCK-8 assay was used to detect the AM viability; the cellular morphology of apoptotic AM was observed under fluorescence microscopy; the apoptosis rate and mitochondrial transmembrane potential of cells were detected by flow cytometry before and after pretreatment with phosphatidyl inositol 3-kinase (PI3K) inhibitor LY294002; Western blot was used to detect the expression of apoptosis-related proteins Bax, Bcl-2, p-PI3K and p-AKt. Resluts: The survival rate of AM was decreased in a time-dose relationship after NS exposure. With LY294002 pretreatment, the mitochondrial transmembrane potential level and the expressions of p-PI3K, p-AKt and Bcl-2 were decreased, the expression of Bax and the apoptosis rate were increased. Conclusion: Our data suggested that the activation of PI3K/AKt signaling pathway played an important role in NS-induced apoptosis in alveolar macrophages.


Assuntos
Apoptose , Macrófagos Alveolares , Fosfatidilinositol 3-Quinase , Proteínas Proto-Oncogênicas c-akt , Transdução de Sinais , Dióxido de Silício , Apoptose/efeitos dos fármacos , Poluentes Ambientais/toxicidade , Humanos , Macrófagos Alveolares/efeitos dos fármacos , Macrófagos Alveolares/enzimologia , Fosfatidilinositol 3-Quinase/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Dióxido de Silício/toxicidade
20.
Huan Jing Ke Xue ; 41(4): 1589-1593, 2020 Apr 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608664

RESUMO

The condensable particle matter (CPM) from coal-fired power plants has attracted significant attention for its potential influence on air quality. The knowledge of CPM emissions from coal-fired power plants is limited. In this study, CPM was collected at the inlet and outlet of wet flue gas desulfurization (WFGD) and the outlet of wet electrostatic precipitator (WESP) using in-direct dilution method. Both mass concentration and water-soluble ions of CPM were analyzed after sampling. The gas precursors were measured at the same time. We showed that gas precursors such as HCl, HNO3, SO3, and NH3 significantly contributed to CPM from coal-fired power plants. As the temperature of flue gas decreased, these gas precursors were observed to form CPM. The major components of CPM were water-soluble ions such as SO42-, Cl-, NO3-, and NH4+. WFGD and WESP could reduce the CPM gas precursors. Therefore, CPM concentrations after WFGD and WESP of the five tested coal-fired power plants were reduced by 27% and 45%, respectively. In addition, the condensation of SO3 increased SO42- concentration but reduced Cl- and NO3- contents. Finally, SO42- was found to be the major water-soluble ion of CPM.

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