Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 27
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Curr Top Med Chem ; 19(20): 1850-1866, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31448711

RESUMO

Notwithstanding substantial improvements in diagnosis and treatment, Heart Failure (HF) remains a major disease burden with high prevalence and poor outcomes worldwide. Natriuretic Peptides (NPs) modulate whole cardiovascular system and exhibit multiple cardio-protective effects, including the counteraction of the Renin-Angiotensin-Aldosterone System (RAAS) and Sympathetic Nervous System (SNS), promotion of vasodilatation and natriuresis, and inhibition of hypertrophy and fibrosis. Novel pharmacological therapies based on NPs may achieve a valuable shift in managing patients with HF from inhibiting RAAS and SNS to a reversal of neurohormonal imbalance. Enhancing NP bioavailability through exogenous NP administration and inhibiting Neutral Endopeptidase (NEP) denotes valuable therapeutic strategies for HF. On the one hand, NEP-resistant NPs may be more specific as therapeutic choices in patients with HF. On the other hand, NEP Inhibitors (NEPIs) combined with RAAS inhibitors have proved to exert beneficial effects and reduce adverse events in patients with HF. Highly effective and potentially safe Angiotensin Receptor Blocker Neprilysin Inhibitors (ARNIs) have been developed after the failure of NEPIs and Vasopeptidase Inhibitors (VPIs) due to lacking efficacy and safety. Therapeutic progress and knowledge basis on the NP system in HF are summarized in the current review.


Assuntos
Inibidores Enzimáticos/farmacologia , Insuficiência Cardíaca/tratamento farmacológico , Peptídeos Natriuréticos/farmacologia , Animais , Inibidores Enzimáticos/metabolismo , Insuficiência Cardíaca/metabolismo , Humanos , Peptídeos Natriuréticos/metabolismo , Neprilisina/antagonistas & inibidores , Neprilisina/metabolismo , Sistema Renina-Angiotensina/efeitos dos fármacos
2.
J Geriatr Cardiol ; 16(6): 458-467, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31308838

RESUMO

Background: Clearance of coronary arterial thrombosis is necessary in patients with acute ST-segment elevation myocardial infarction (STEMI) undergoing urgent percutaneous coronary intervention (PCI). There is currently no highly-recommended method of thrombus removal during interventional procedures. We describe a new method for opening culprit vessels to treat STEMI: intracoronary arterial retrograde thrombolysis (ICART) with PCI. Methods & Results: Eight patients underwent ICART. The guidewire was advanced to the distal coronary artery through the occlusion lesion. Then, we inserted a microcatheter into the distal end of the occluded coronary artery over the guidewire. Urokinase (5-10 wu) mixed with contrast agents was slowly injected into the occluded section of the coronary artery through the microcatheter. The intracoronary thrombus gradually dissolved in 3-17 min, and the effect of thrombolysis was visible in real time. Stents were then implanted according to the characteristics of the recanalized culprit lesion to achieve full revascularization. One patient experienced premature ventricular contraction during vascular revascularization, and no malignant arrhythmias were seen in any patient. No reflow or slow flow was not observed post PCI. Thrombolysis in myocardial infarction flow grade and myocardial blush grade post-primary PCI was 3 in all eight patients. No patients experienced bleeding or stroke. Conclusions: ICART was accurate and effective for treating intracoronary thrombi in patients with STEMI in this preliminary study. ICART was an effective, feasible, and simple approach to the management of STEMI, and no intraprocedural complications occurred in any of the patients. ICART may be a breakthrough in the treatment of acute STEMI.

3.
Biomark Med ; 13(6): 457-466, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30785341

RESUMO

Aim: MiR-335-5p expression might induce endothelial cells (ECs) aging and target inhibit sKlotho. This study aimed to investigate whether oxidative stress evoked miR-335-5p expression and whether miR-335-5p-regulated ECs function through sKlotho. Methods: The expression of miR-335-5p was detected in human umbilical vein endothelial cells treated with H2O2. Subsequently, endothelial function and sKlotho expression were measured in human umbilical vein endothelial cells treated with H2O2 and transfected with miR-335-5p mimics or inhibitor sequences. Vector containing reporting system of sKlotho3'- untranslated region with a miR-335-5p-binding site was constructed. Results: H2O2 stimulation significantly increased miR-335-5p expression. Force overexpression miR-335-5p suppress ECs function and sKlotho expression. MiR-335-5p target regulated sKlotho. Conclusion: MiR-335-5p might serve as a negative factor for endothelial homeostasis and a potential treatment target for atherosclerosis.

5.
Aging Clin Exp Res ; 28(3): 475-82, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26264248

RESUMO

BACKGROUND AND AIMS: Previous studies have confirmed that chronic kidney disease (CKD) influences the cognitive function of adults. However, few studies focused the relationships among the very old. The purpose of this study was to determine whether CKD is associated with cognitive impairment among nonagenarians and centenarians in China. METHOD: This work was conducted as a cross-sectional study. 767 unrelated Chinese nonagenarians and centenarians participated in the study. The 30-item mini-mental state examination (MMSE) was used to assess cognitive function. Estimated glomerular filtration rate (eGFR) was estimated using the Modification of Diet in Renal Disease equation (Chinese version). CKD was defined as eGFR less than 60 mL/min/1.73 m(2). RESULTS: The cohort was 68.2 % female and mean (SD) age was 93.8 (3.5) years. There were 113 (14.7 %) and 577 (75.2 %) prevalent cases of CKD and cognitive impairment, respectively. After adjusting for sociodemographic variables, multivariate logistic regression showed CKD was not significantly associated with cognitive impairment (OR 0.81, 95 % CI 0.49-1.33). When other disease-related variables were adjusted, the result remained substantially unchanged. However, age, gender, education and income may largely or entirely explain the lack of association between CKD and cognitive impairment as measured according to the MMSE. CONCLUSION: Among Chinese nonagenarians and centenarians, CKD was not directly correlated with cognitive impairment.


Assuntos
Disfunção Cognitiva/epidemiologia , Insuficiência Renal Crônica/epidemiologia , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Modelos Logísticos , Masculino , Testes de Estado Mental e Demência
6.
Open Rheumatol J ; 8: 9-12, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25067964

RESUMO

To our knowledge, the possible unveiled interaction between adult-onset Still's disease (AOSD) with autoimmune thyroid disease (AITD) has never been reported although it is well established that systemic autoimmune disease may usually occur in relation to AITD. As increasingly clear links of AITD with other autoimmune disease such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and primary Sjögren's syndrome (pSS) have been reported, and the incidence of AOSD concurrent AITD draws our attention rapidly. In this study, we searched relevant literatures published in the past 30 years to explore that condition.

7.
PLoS One ; 9(2): e89827, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24587064

RESUMO

BACKGROUND: Little is known so far on the lipid profile in polymyositis (PM) patients. Our aim is to identify lipid profiles in untreated patients with early PM, to assess the association between lipid profiles and C-reactive protein (a sensitive marker of inflammation) in these patients. METHODS AND FINDINGS: This work was conducted as a case-control study. Sixty untreated patients with PM and 60 age- and sex-matched healthy controls were included. The duration of PM was less than six months, and none of them had received intermittent or regular corticosteroids or disease-modifying antirheumatic drugs or biological agents prior to the study. Triglyceride (TG), total cholesterol (TC), LDL-cholesterol (LDL-C), and HDL-cholesterol (HDL-C), and C-reactive protein (CRP) were assessed using standard techniques. Thirty patients (50%) had a decreased level of HDL-C and 47% had an increased level of TG. The levels of HDL-C, LDL-C, and TC in PM were significantly lower than in controls (P<0.001, P<0.01, P<0.001, respectively). The level of TG was significantly higher in PM than in controls (P<0.001). The level of very low LDL-cholesterol (VLDL-C), and the ratios of VLDL-C/LDL-C, TC/HDL-C, and LDL-C/HDL-C were significantly higher than in controls (all P<0.001). Serum CRP levels correlated negatively with HDL-C (r = -0.352, P = 0.006) and TC (r = -0.262, P = 0.043). After adjustment for age, gender, smoking, drinking, body mass index, and pulmonary fibrosis/infection, linear regression model demonstrated that CRP is associated with HDL-C among PM patients (P = 0.028). CONCLUSIONS: Dyslipidemia is a common feature in patients with PM that is characterized by a decrease in HDL-C and an increase in TG, suggesting a high risk of atherosclerosis. The Inflammatory condition in PM may account for the metabolism of HDL-C.


Assuntos
Biomarcadores/sangue , Proteína C-Reativa/metabolismo , Dislipidemias/etiologia , Polimiosite/sangue , Polimiosite/complicações , Adulto , Fatores Etários , Idoso , Consumo de Bebidas Alcoólicas , Índice de Massa Corporal , Estudos de Casos e Controles , China , Colesterol/sangue , Dislipidemias/sangue , Feminino , Humanos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Fatores Sexuais , Fumar , Triglicerídeos/sangue
8.
J Renin Angiotensin Aldosterone Syst ; 15(1): 88-96, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23435582

RESUMO

OBJECTIVE: This meta-analysis was performed to assess whether arginine supplementation could reduce preeclampsia or eclampsia incidence and improve the outcomes of hypertensive disorders in pregnancy, and to evaluate the safety of L-arginine supplementation. METHODS: The Cochrane Central Register of Controlled Trials (2011), MEDLINE (1980-2011) and Embase (1980-2011) were searched through July 2012, and randomized controlled trials (RCTs) comparing intravenous and/or oral L-arginine supplementation with placebo, or RCTs comparing any treatment with arginine were included. Qualities of RCTs were assessed with the Jadad method. Meta-analyses were performed with fixed- or random-effects models according to heterogeneity of studies. RESULTS: Data from seven RCTs involving 916 patients were enrolled. The meta-analysis showed L-arginine was more effective in reducing preeclampsia or eclampsia incidence (odds ratio 0.384; 95% confidence limits 0.25, 0.58) than the placebo; meanwhile, L-arginine could prolong pregnancy weeks (MD 11.54; 95% CL 5.23, 17.85) than placebo; and its effect on blood pressure was unbalanced (diastolic pressure (MD 4.86; 95% CL 4.19, 5.52) and systolic pressure (MD 3.20; 95% CL -1.54, 7.94)) while the difference in increased neonatal weight (MD 256.24; 95% CL -28.66, 541.13) was not clear. Three of these studies reported some adverse effects, and no teratogenic or lethal effects were noted. CONCLUSION: This study demonstrates L-arginine supplementation is superior to placebo in lowering diastolic pressure and prolonging pregnancy in patients with gestational hypertension with or without proteinuria, but the effect on lowering systolic pressure and increasing neonatal weight was not statistically significant.


Assuntos
Arginina/uso terapêutico , Hipertensão Induzida pela Gravidez/tratamento farmacológico , Peso ao Nascer/efeitos dos fármacos , Suplementos Nutricionais , Feminino , Humanos , Recém-Nascido , Pré-Eclâmpsia/prevenção & controle , Gravidez , Ensaios Clínicos Controlados Aleatórios como Assunto
9.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 30(4): 808-11, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24059061

RESUMO

This study aims to assess the expression of Klotho and insulin-like growth factor-1 (IGF-1) and the association between Klotho and IGF-1 in rats with dementia model. Thirty rats were randomly divided into three groups. Morris water maze was used to investigate the learning and memory functions, and enzyme linked immunosorbent assay was used to analyze the levels of Klotho and IGF-1. Klotho and IGF-1 levels in the model group were lower than those in other 2 groups. Morris water maze test showed that the model group had longer escape latency times and shorter step platform times compared to other groups. Line correlation model demonstrated that Klotho level was positively correlated with IGF-1 level in rats with dementia (P= 0. 029). The levels of Klotho and IGF-1 both reduced at hippocampus in rats with dementia model, suggesting that it may be a close relationship between Klotho and IGF-1 in the pathogenesis of dementia.


Assuntos
Demência/metabolismo , Glucuronidase/metabolismo , Hipocampo/metabolismo , Fator de Crescimento Insulin-Like I/metabolismo , Animais , Feminino , Glucuronidase/genética , Fator de Crescimento Insulin-Like I/genética , Masculino , Aprendizagem em Labirinto , Memória/fisiologia , Ratos , Ratos Wistar
10.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 30(3): 588-91, 2013 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-23865324

RESUMO

The study was aimed to assess the effect of Klotho gene and sinoatrial node pacing channel gene (HCN4 and HCN2) for studying sick sinus syndrome, with Klotho gene under the interference of Plasmid-mediated short hairpin RNA. Twenty-five C57BL/6J mice were divided into four groups, i. e, plasmid shRNA 24h group, plasmid shRNA 12h group, sodium chloride 24h group and sodium chloride 12h group. Plasmid shRNA 50microL (1microg/microL) and sodium chloride 50microl were respectively injected according to mice vena caudalis into those in plasmid shRNA group and sodium chloride group. After 12h or 24h respectively, all mice were executed and their sinoatrial node tissues were cut. The mRNA of Klotho, HCN4 and HCN2 gene were detected by RT-PCR. The results of RT-PCR showed that Klotho, HCN4 and HCN2 mRNA levels were lower compared with those in sodium chloride 12h group after 12h interference interval. The results indicated that there might be the a certain relationship between Klotho gene and sinoatrial node pacing channel gene.


Assuntos
Glucuronidase/genética , Canais Disparados por Nucleotídeos Cíclicos Ativados por Hiperpolarização/genética , Plasmídeos/genética , Canais de Potássio/genética , RNA Interferente Pequeno/genética , Nó Sinoatrial/fisiologia , Animais , Canais Disparados por Nucleotídeos Cíclicos Ativados por Hiperpolarização/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Canais de Potássio/metabolismo , Interferência de RNA , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Nó Sinoatrial/metabolismo , Nó Sinoatrial/fisiopatologia
11.
J Aging Health ; 24(8): 1298-319, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23006424

RESUMO

OBJECTIVE: The authors investigated the relationship of activities of daily living (ADL) disability with medical diseases and geriatric conditions among nonagenarians and centenarians. METHOD: Cross-sectional, n = 870 Chinese (age range: 90-108 years). Self-reported medical diseases and geriatric-specific conditions were obtained by face-to-face interviews. Biomedical measurements included systolic/diastolic blood pressure, BMI (body mass index), albumin, fasting glucose, creatinine clearance, hemoglobin, and lipid panel. RESULTS: In bivariate analyses, 4 of 7 geriatric conditions (hearing problems, falls, cognitive impairment, fracture), and 6 of 11 biomedical measurements (fasting glucose, cholesterol, LDL, creatinine clearance, hemoglobin, albumin), but none of 10 medical diseases and no degree of comorbidities, were associated with ADL disability. In four different multivariate logistic regression models, two geriatric conditions (impaired cognition, fracture) were significant in three models, respiratory disease in one model, and fasting glucose in two models. DISCUSSION: This study emphasizes the importance of geriatric conditions and their association with ADL disability among the oldest-old adult population.


Assuntos
Atividades Cotidianas , Envelhecimento/fisiologia , Pessoas com Deficiência/estatística & dados numéricos , Avaliação Geriátrica , Fatores Etários , Idoso de 80 Anos ou mais , China , Estudos Transversais , Feminino , Humanos , Masculino , Pesquisa Qualitativa , Autorrelato
12.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 28(3): 526-30, 2011 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-21774216

RESUMO

The aim of the studies was to investigate klotho gene effect on the endothelial dysfunction of spontaneously hypertensive rats (SHR). In this study, ten SHR and ten normal Wistar rats, all 22 week old, were prepared. After given intraperitoneal anesthesia, the rats' brains, lungs, hearts, kidneys and aortas were removed. The identification was made by means of real-time polymerase chain reaction (Real-time PCR) and Enzyme-Linked Immunosorbent Assay (ELISA). Compared with the normal group, the klotho mRNA and protein in SHR were less than those in the control group with normal corresponding values, while Endothelin-1 (ET-1)'s mRNA and protein were more than those of normal group. The analysis of the correlation of mRNA and protein in heart and aorta revealed that klotho gene was negatively correlated to ET-1. The results showed that klotho significantly decreased in SHR, which might be influenced by hypertension-induced damage on the endothelial function.


Assuntos
Envelhecimento/genética , Endotelina-1/metabolismo , Endotélio Vascular/fisiopatologia , Glucuronidase/genética , Hipertensão/genética , Animais , Endotelina-1/genética , Glucuronidase/metabolismo , Hipertensão/fisiopatologia , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Endogâmicos SHR
13.
Clin Rheumatol ; 30(4): 449-58, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20857158

RESUMO

Studies confirming a possible relationship of polymyositis within thyroid dysfunction, either hypothyroidism or hyperthyroidism, are hardly available. To define the association, identify clinical, laboratory, electromyographic, and pathologic features in polymyositis (PM) patients with hypothyroidism or hyperthyroidism, we conducted a MEDLINE and Chinese biomedicine database search to identify relevant literature published in the past 25 years. Seventeen cases were included. All patients were female (10 hypothyroidism patients, seven hyperthyroidism patients). The mean (SD) age of PM, hypothyroidism, and hyperthyroidism at diagnosis was 54.8 (16.7), 55.5 (16.5), and 32.7 years (10.2), respectively. PM diagnosis can precede or parallel hypothyroidism while PM may occur following the diagnosis of hyperthyroidism. The most common comorbidities were malignant tumors in these disorders, including thymoma, colon cancer, and thyroid cancer. Muscle weakness was described in 100% of patients. Other common manifestations included muscles' atrophy and pain, deep tendon reflexes, polyarthralgia, and dysphagia. Most patients had markedly elevated creatine kinase and the presence of anti-Sjogren's syndrome A (SSA) antibodies was also found in two cases. Malignancy associated with PM may more frequently occur in hypothyroidism than in hyperthyroidism. The abnormalities on electromyography and biopsy did not differ from those findings of PM. Therapy consisting of corticosteroids, thyroid hormone, or anti-thyroid drugs was administrated; however, poor prognosis seemed to be associated with malignant tumors as well as older age and the presence of anti-SSA antibodies. It is reasonable to suggest that those patients should be routinely evaluated for thyroid function, especially in older female and patients suffering from cancers.


Assuntos
Doença de Hashimoto/complicações , Hipertireoidismo/complicações , Hipotireoidismo/complicações , Polimiosite/complicações , Prednisona/administração & dosagem , Adulto , Anti-Inflamatórios/administração & dosagem , Estudos de Coortes , Feminino , Doença de Hashimoto/tratamento farmacológico , Doença de Hashimoto/fisiopatologia , Humanos , Hipotireoidismo/tratamento farmacológico , Hipotireoidismo/fisiopatologia , Pessoa de Meia-Idade , Polimiosite/tratamento farmacológico , Polimiosite/fisiopatologia , Tiroxina/administração & dosagem
14.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 41(6): 1029-33, 2010 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-21265109

RESUMO

OBJECTIVE: To investigate the accuracy of dual source computed tomography (DSCT) coronary angiography in detecton of coronary artery stenoses in patients older than 60 years. METHODS: The study was performed in 102 patients older than 60 years who were suspected of coronary artery stenoses, and without any contraindications to CT Scan and iodnated contrast agents. These patients underwent DSCT, as well as selective coronary angiography (SCA). All the data of DSCT were compared with the results of SCA which were regarded as gold standard to assess the sensitivity, specificity, positive predictive value, negative predictive value and accuracy of DSCT in classifying coronary artery stenoses in old patients. RESULTS: In these 102 patients, DSCT indicated 56 patients with coronary artery stenoses and SCA indicated 52 patients. DSCT correctly classified 51 patients out of 52 patients who had significant coronary artery stenoses. The sensitivity of DSCT was 98.1%, specificity was 90.0%, positive predictive value was 91.1%, negative predictive value was 97.8%, and accuracy was 94.1%. DSCT also assessed and measured 408 lesions in all the patients. The sensitivity of DSCT was 95.8%, specificity was 96.2%, positive predictive value was 88.5%, negative predictive value was 98.7%, and accuracy was 96.1%. CONCLUSION: DSCT appears to be a useful method for the detection of coronary artery stenoses with a high accuracy in elder patients more than 60-year.


Assuntos
Angiografia Coronária/métodos , Estenose Coronária/diagnóstico por imagem , Intensificação de Imagem Radiográfica/métodos , Tomografia Computadorizada por Raios X/métodos , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
15.
Int J Geriatr Psychiatry ; 25(7): 697-703, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20033902

RESUMO

OBJECTIVE: Previous research has suggested that social support and functional status influence the subjective well-being of the elderly. However, few studies have investigated whether these associations influence well-being in the very old. METHODS: In this population-based, cross-sectional study, 1401 persons aged 90 years or over were located in Du Jiang Yan city, Sichuan, China. Among them 732 were willing to participate and provided data on a battery of standardized questionnaire inventories. The 23-item Philadelphia Geriatric Center Morale Scale (PGCMS) was examined as an index of subjective well-being. Functional status was assessed using the physical self-maintenance scale (PSMS) and the instrumental activities of daily living (IADL) scales. The mini-mental state examination (MMSE) was used to measure cognitive function. Social support was assessed using the Family Adaptation, Partnership, Growth, Affection, and Resolve (APGAR) scale and the social support requirement scale (SSRS). RESULTS: The mean age was 93.7 +/- 3.4 years for the participants, of whom 68% were female. PGCMS total scores were significantly correlated with the Family APGAR and Objective Support subscale of the SSRS, as well as with scores on the MMSE and PSMS scales. However, in a multiple regression analysis, only MMSE and Family APGAR scores were independent predictors of PGCMS scores. CONCLUSION: Both social support from family members and cognitive function appear to be key factors associated with quality of life among the very old in China. Further research is needed among very old populations to confirm the importance of these variables and to examine potential cross-cultural differences.


Assuntos
Nível de Saúde , Apoio Social , Atividades Cotidianas , Idoso de 80 Anos ou mais , Escalas de Graduação Psiquiátrica Breve , China , Estudos Transversais , Avaliação da Deficiência , Feminino , Avaliação Geriátrica , Humanos , Masculino , Moral , Qualidade de Vida , Autoimagem , Inquéritos e Questionários
16.
Artigo em Chinês | MEDLINE | ID: mdl-19334553

RESUMO

This study sought to explore the relationship between the change in ventricular electrical remodeling caused by mechano-electrical feedback and the expression of L-type Ca2+ -channel and/or sarcoplasmic reticulum Ca2+ -ATPase in the rabbits with congestive heart failure (CHF). 138 rabbits were divided into two groups (CHF and control). We measured the ventricular monophasic action potential duration (MAPD) and ventricular effective refractory period (VERP) during ventricular pacing at the stimulus frequency of 220/240/260 bpm in these rabbits. Rapid atrial pacing (260/min) was given for 30 minutes. The MAPD and VERP were measured again. Then ventricular fibrillation was induced by S1S2S3 program stimulation. We extracted the total RNA from the myocardium respectively and detected L-type Ca2+ -channel mRNA and sarcoplasmic reticulum Ca2+ -ATPase mRNA by use of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). In group CHF, with the increasing of preload/afterload, L-type Ca2+ -channel mRNA was up regulated after rapid atrial pacing when compared with that in control groups (P < 0.05). There was no significant change in sarcoplasmic reticulum Ca2+ -ATPase mRNA after rapid atrial pacing when compared with controls (P > or = 0.05). The changes in MAPD90 and VERP were related with the extent of L-type Ca2+ -channel mRNA up regulation. But the changes in MAPD90 and VERP were not significantly related with the extent of sarcoplasmic reticulum Ca2+ -ATPase mRNA up regulation. These findings suggest that Mechano-Electrical Feedback could increase the regional changes of ventricular electrical remodeling in rabbits with CHF and so to predispose them to ventricular arrhythmia. The changes may be related with the up regulation of L-type Ca2+ -channel mRNA, but not with sarcoplasmic reticulum Ca2+ -ATPase mRNA.


Assuntos
Canais de Cálcio Tipo L/metabolismo , Condutividade Elétrica , Insuficiência Cardíaca/fisiopatologia , Remodelação Ventricular , Potenciais de Ação , Animais , Canais de Cálcio Tipo L/genética , Estimulação Cardíaca Artificial , Eletrofisiologia , Feminino , Insuficiência Cardíaca/metabolismo , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Coelhos , Distribuição Aleatória , Período Refratário Eletrofisiológico , Retículo Sarcoplasmático/genética , Retículo Sarcoplasmático/metabolismo
17.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 38(3): 447-50, 512, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17593828

RESUMO

OBJECTIVE: To investigate the feasibility of cultured rat ventricular myocytes taking the place of acutely enzymatic isolated myocardia for study in the pacemaker current electrophysiology. METHODS: By using patch-clamp technique, the whole-cell pacemaker currents were recorded to study the difference of pacemaker current electrophysiology between neonatal rat ventricular myocytes isolated acutely and maintained in cell culture. RESULTS: The cell membrane capacitances (Cm) were significantly decreased in myocardia even upon short term of cell culture. The Cm of myocardia cultured 1-3 day were (43 +/- 3) pF, with decreased 23% compared to acute enzymatic isolated cells C(56+/-7) pF, P<0. 013. It seemed like the Cm increased slightly upon cell cultured time, but no significant difference appearing among various culture stages (P > 0. 05) . No significant difference of pacemaker current electrophysiological character, such as reverse potential, current density, active threshold and V0.5, was detected from different cell group (P>0. 05). CONCLUSION: The cultured myocardium can take place of acute enzymatic isolated myocardium used to study in pacemaker current. The cells cultured no more than one week may be more suitable to this kind of electrophysiological experiment.


Assuntos
Condutividade Elétrica , Fenômenos Eletrofisiológicos , Enzimas/metabolismo , Células Musculares/citologia , Miocárdio/citologia , Marca-Passo Artificial , Função Ventricular , Animais , Animais Recém-Nascidos , Membrana Celular/metabolismo , Separação Celular , Células Cultivadas , Capacitância Elétrica , Estudos de Viabilidade , Feminino , Ventrículos do Coração/citologia , Masculino , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley
18.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 38(3): 484-7, 2007 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-17593838

RESUMO

OBJECTIVE: To describe the social functions of the longevous elderly population in Dujiangyan. METHODS: A questionnaire survey was undertaken in Dujiangyan in 875 elderly people who were over 90 years old. RESULTS: Poor social economic status was prevalent in the longevous elderly population. The elderly people had limited social contacts except from their families. The daily lives of 65. 0% of the longevous elderly were taken care of by their families. More than 81. 8% of the longevous elderly people were financially supported by their families. About 89. 2% of the longevous elderly people had accessed to spiritual supports in difficult circumstances, among 88. 7% came from families. CONCLUSION: Thanks to the family supports, the longevous people in Dujiangyan have maintained good social functions.


Assuntos
Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Avaliação Geriátrica/estatística & dados numéricos , Expectativa de Vida , Comportamento Social , Apoio Social , Idoso de 80 Anos ou mais , China , Redes Comunitárias/estatística & dados numéricos , Escolaridade , Família , Feminino , Habitação/estatística & dados numéricos , Humanos , Renda/estatística & dados numéricos , Masculino , Espiritualidade
19.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 24(1): 136-9, 181, 2007 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-17333908

RESUMO

To assess the changes of sarcolemma Na+/K+ ATPase (CMNKA) and sarcoplasmic reticulum membrane Ca2+ ATPase (SERCA) activities after stem cells transplantation in heart failure. Rabbit was used as heart failure model by intravenously injecting adriamycin. Autologous bone marrow mononuclear cells (BMCs), bone marrow mesenchymal stem cells (MSCs) or skeletal myoblasts (SMs) were introduced into coronary arteies through the root of aorta when two balloons occluding just above sinus of Valsalva. After 4 weeks, left ventricular ejection fraction (LVEF)was evaluated by echocardiography, and the activities of CMNKA and SERCA were measured by colorimeter. In BMCs (n=8)and MSCs (n=8) group, LVEF were significantly improved (P < 0.05). No significant improvement were seen in SMs group (n=6) compared to sham group (n=8). The CMNKA activity in all stem cells groups was significantly increased compared to sham group (P < 0.05). Meanwhile, in comparison with sham group, the incremental tendencies of SERCA activity were seen in stem cells groups. In conclusion, stem cells transplantation could increase the activities of CMNKA and SERCA in heart failure, a possible mechanism to improve heart function.


Assuntos
Insuficiência Cardíaca/terapia , Sarcolema/enzimologia , ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismo , Transplante de Células-Tronco , Animais , Doxorrubicina , Feminino , Insuficiência Cardíaca/induzido quimicamente , Insuficiência Cardíaca/enzimologia , Masculino , Miocárdio/enzimologia , Coelhos , Distribuição Aleatória
20.
Cell Biol Int ; 30(7): 576-82, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16731012

RESUMO

To investigate whether stem cell transplantation affects ventricular electrophysiology in vivo, either autologous bone marrow mesenchymal stem cells or skeletal myoblast cells were transplanted via a catheter into a doxorubicin-treated failing heart. Four weeks after transplantation, electrophysiological investigation showed that transplantation of either cell type prolonged the local activation time and increased the activation time dispersion. In the stem cell transplantation groups, a positive correlation was demonstrated between activation time dispersion and the number of stem cell-derived cells in the pacing site. It is concluded that transplantation of either mesenchymal stem cells or skeletal myoblast cells might exacerbate abnormalities of local ventricular conduction in the doxorubicin-treated failing heart.


Assuntos
Cardiomiopatia Dilatada/fisiopatologia , Transplante de Células-Tronco Mesenquimais , Função Ventricular Esquerda/fisiologia , Animais , Cardiomiopatia Dilatada/induzido quimicamente , Doxorrubicina , Eletrofisiologia , Mioblastos/transplante , Miocárdio/patologia , Coelhos , Transplante Autólogo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA