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1.
J Clin Transl Hepatol ; 11(1): 110-117, 2023 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-36406330

RESUMO

Background and Aims: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death and ranks sixth in terms of incident cases worldwide. The purpose of this study was to develop an effective and sensitive method to distinguish liver cancer tissues from normal tissues in HCC patients. Integrin α6 is a promising cell surface target for molecular imaging of HCC, where it is overexpressed and is a prognostic biomarker. We previously identified an integrin α6-targeted peptide CRWYDENAC (RWY) that has been used for positron emission tomography (PET) imaging of HCC in mouse models. Methods: We labeled the integrin α6-targeted RWY peptide with cyanine 7 (Cy7) to form an optical probe (Cy7-RWY) for near infrared fluorescent (NIRF) and photoacoustic (PA) imaging in HCC. Mice transplanted with subcutaneous HCC-LM3 or orthotopic HCC-H22 cells that overexpressed integrin α6 were intravenously injected with Cy7-RWY and its corresponding Cy7-control. NIRF and PA images of mice were collected from 0 to 48 h after injection. Results: Both NIRF and PA signals started to accumulate in the tumor 2 h after injection of Cy7-RWY and peaked at 24 h. Conclusions: Cy7-RWY is a promising optical probe for NIRF and PA imaging of HCC in mice, and has potential clinical application for HCC detection.

2.
Artigo em Inglês | MEDLINE | ID: mdl-36383777

RESUMO

Direct chemical vapor deposition of graphene on semiconductors and insulators provides high feasibility for integration of graphene devices and semiconductor electronics. However, the current methods typically rely on high temperatures (>1000 °C), which can damage the substrates. Here, a growth method for high-quality large-area graphene at 300 °C is introduced. A multizone furnace with gradient temperature control was designed according to a computational fluid dynamics model. The crucial roles of the chamber pressure in the film continuity and hydrogen composition in the graphene defect density at low temperature were revealed. As a result, a uniform graphene film with the Raman ratio ID/IG = 0.08 was obtained. Furthermore, a technique of laminating single-crystal Cu foil as a sacrificial layer on the substrate was proposed to realize transfer-free growth, and a wafer-scale graphene transistor array was demonstrated with good performance consistency, which paves the way for mass fabrication of graphene devices.

3.
Int J Biol Macromol ; 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36403764

RESUMO

The genus Streptomyces comprises the most important chitin decomposers in soil and revealing their chitinolytic machinery is beneficial for the conversion of chitinous wastes. Streptomyces sp. SCUT-3, a chitin-hydrolyzing and a robust feather-degrading bacterium, was isolated previously. The potential chitin-degrading enzymes produced by SCUT-3 were analyzed in the present study. Among these enzymes, three chitinases were successfully expressed in Pichia pastoris at comparatively high yields of 4.8 U/mL (SsExoChi18A), 11.2 U/mL (SsExoChi18B), and 17.8 U/mL (SsEndoChi19). Conserved motifs and constructive 3D structures of these three exo- and endochitinases were also analyzed. These chitinases hydrolyzed colloidal chitin to chitin oligomers. SsExoChi18A showed apparent synergic effects with SsEndoChi19 in colloidal chitin and shrimp shell hydrolysis, with an improvement of 29.3 % and 124.9 %, respectively. Compared with SsExoChi18B and SsEndoChi19, SsExoChi18A exhibited the strongest antifungal effects against four plant pathogens by inhibiting mycelial growth and spore germination. This study provided good candidates for chitinous waste-processing enzymes and antifungal biocontrol agents. These synergic chitin-degrading enzymes of SCUT-3 are good targets for its further genetical modification to construct super chitinous waste-degrading bacteria with strong abilities to hydrolyze both protein and chitin, thereby providing a direction for the future path of the chitinous waste recycling industry.

4.
Nutrients ; 14(21)2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36364921

RESUMO

Placental extract has been used for skin care and delaying skin aging. Cow placenta is an abundant resource with a large mass, which has not been harnessed effectively. Cow placenta extract (CPE) has the functions of antioxidation, anti-inflammatory, promoting growth and development, and promoting hair growth. However, little is known about the effect of oral administration of cow placenta extract on skin conditions. Therefore, the present study aimed to investigate the antioxidant capacity of CPE in vitro and in vivo and its protective effect on d-galactose (D-gal) induced skin aging in mice. The results showed that CPE had strong free radical scavenging, reducing and metal chelating activities. CPE can increase the activity of catalase (CAT), glutathione peroxidase (GSH-Px), peroxidase (POD), superoxide dismutase (SOD), and the content of glutathione (GSH), decrease the content of malondialdehyde (MDA). Moreover, CPE can decrease the gene and protein expression of matrix metalloproteinase 1a (MMP-1a) and matrix metalloproteinase 3 (MMP-3) and increase the expression of transforming growth factor-ß (TGF-ß) and tissue inhibitor of metalloproteinase 1 (TIMP-1) of mouse skin. Histopathological analysis showed CPE reduced the collagen damage caused by D-gal, increased collagen synthesis and reduced its degradation to delay skin aging.


Assuntos
Antioxidantes , Envelhecimento da Pele , Animais , Bovinos , Feminino , Camundongos , Gravidez , Antioxidantes/farmacologia , Antioxidantes/metabolismo , Galactose/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Estresse Oxidativo , Placenta/metabolismo , Extratos Vegetais/farmacologia , Superóxido Dismutase/metabolismo , Inibidor Tecidual de Metaloproteinase-1/metabolismo
5.
RSC Adv ; 12(45): 29003-29009, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36320726

RESUMO

The magnetic semiconductor in a two-dimensional system is a major subject for both theoretical and experimental investigations. Here we report the synthesis of a new quaternary manganese chalcogenide KMnCuTe2, which shows layered structure and antiferromagnetic (AFM) semiconducting features. Single crystals of KMnCuTe2 were obtained using a self-flux method and based on single-crystal X-ray diffraction, KMnCuTe2 adopts the ThCr2Si2-type structure composed of edge-sharing tetrahedral layers separated by K+ cations. The Mn and Cu atoms randomly distribute in the centre of tetrahedral units. Attributed to the large radius of Te, KMnCuTe2 has large lattice parameters (a = 4.3115(3) Å and c = 14.9360(20) Å), leading to a long metal-metal distance (3.049 Å) in the tetrahedral layers. Based on the experiments and theoretical calculations, KMnCuTe2 exhibits a G-type AFM interaction with the transition temperature at around 225 K and an indirect semiconducting nature with the band gap of 0.95 eV. The magnetic semiconducting property of KMnCuTe2 is unique in AMnMCh2 systems (A = Li, Na, K, M = Cu, Ag and Ch = S, Se, Te), which could be associated with the large metal-metal distance. Our work not only highlights the role of metal-metal interactions on regulating the properties of ThCr2Si2-type compounds, but also provides a feasible strategy to obtain the layered magnetic semiconductor.

7.
Cell Cycle ; : 1-14, 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-36196460

RESUMO

Abdominal aortic aneurysm (AAA) is a vascular disorder greatly threatening life of the elderly population. Dexmedetomidine (DEX), an α2-adrenergic receptor agonist, has been shown to suppress AAA development. Nevertheless, the signaling pathways that might be mediated by DEX in AAA has not been clarified. Vascular smooth muscle cells (VSMCs) and endothelial cells (ECs) were treated with Angiotensin II (Ang II) to mimic AAA in vitro. BrdU, wound healing, and Transwell assays were utilized for measuring VSMC proliferation and migration. Western blotting was used for evaluating protein levels of contractile VSMC markers, collagens and matrix metalloproteinases (MMPs) in VSMCs as well as apoptosis- and HMGB1/TLR4/NF-κB signaling-related markers in ECs. Cell adhesion molecule expression and monocyte-endothelial adhesion were assessed by immunofluorescence staining and adhesion assays. Flow cytometry was implemented for analyzing EC apoptosis. Hematoxylin-eosin staining and ELISA were used to detect the effect of DEX in vivo. In this study, DEX inhibited Ang II-evoked VSMC phenotype switch and extracellular matrix degradation. DEX suppressed the inflammatory response and apoptosis of ECs induced by Ang II. DEX inhibited HMGB1/TLR4/NF-κB signaling pathway in Ang II-treated ECs. DEX attenuated Ang II-induced AAA and inflammation in mice. Overall, DEX ameliorates Ang II-induced VSMC phenotype switch, and inactivates HMGB1/TLR4/NF-κB signaling pathway to alleviate Ang II-induced EC dysfunction.

8.
Org Lett ; 24(44): 8104-8108, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36286341

RESUMO

Rugosiformisin A, a skeleton-rearranged abietane-type diterpenoid with a spiro[4.5]decane motif, was isolated from Isodon rugosiformis. Its structure was unambiguously established via NMR spectroscopic analysis and single-crystal X-ray diffraction. A bioinspired asymmetric synthesis of rugosiformisin A was achieved in 15 steps with 2.7% overall yield. The synthesis features an iridium-catalyzed asymmetric polyene cyclization and a semipinacol rearrangement.


Assuntos
Diterpenos , Isodon , Isodon/química , Abietanos/química , Espectroscopia de Ressonância Magnética , Extratos Vegetais/química , Esqueleto , Estrutura Molecular , Diterpenos/química
9.
J Phys Condens Matter ; 34(49)2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36252541

RESUMO

Nodal-line superconductor NaAlSi with a transition temperature (Tc) of 7 K has attracted considerable attention in recent years, whereas its Ge counterpart, NaAlGe, does not superconduct down to the lowest temperature regardless of their similar atomic and electrical structures. To tackle this enigma, we resort to the growth of NaAlGe single crystal and characterize its ground state. Interestingly, when hole doped by oxidation or extracting Na, single-crystalline NaAlGe transforms from a semimetal/semiconductor to a superconductor (Tc=1.8∼3.3 K) with zero resistivity and a diamagnetic shielding fraction over 100%, but without a thermodynamic response in heat capacity. Continuous x-ray diffraction reveals a transient new structure with a largercaxis, which is suggested to have arisen from the minor loss of Na and to be responsible for the emergence of the delicate superconductivity. Our findings place NaAlGe on an equal footing with NaAlSi and provide an alternative for studying the intriguing relationship between superconductivity and nodal-line topology.

10.
Exp Neurol ; 359: 114250, 2022 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-36240882

RESUMO

Orexin is a neuropeptide restrictedly synthesized in the hypothalamus, but extensively modulates the whole brain region activity including prefrontal cortex (PFC), and involved in the pathophysiology of psychiatric disorders. GABAergic interneurons in the mPFC are a promising pharmacological target for developing antidepressant therapies. Here, we examined the effects of the orexin on GABAergic transmission onto pyramidal neurons in the deep layers of the mPFC. We found that bath application of orexin dose-dependently increased the amplitude of evoked IPSCs (eIPSCs). Orexin increased the frequency but not the amplitude of miniature IPSCs (mIPSCs). Ca2+ influx through T-type voltage-gated Ca2+ channels is required for orexin-induced increases in GABA release. We also found orexin increases GABA release probability and the number of releasable vesicles. Orexin depolarizes somatostatin (Sst) interneurons without effects on the firing rate of action potentials (APs) of Sst interneurons. Orexin-induced depolarization of Sst interneurons is independent of extracellular Na+, Ca2+ and T-type Ca2+ channels, but requires inward rectifier K+ channels (Kirs). The present study suggests that orexin enhances GABAergic transmission onto mPFC pyramidal neurons through inhibiting Kirs on Sst interneurons, which further depolarizes interneurons leading to increase in Ca2+ influx via T-type Ca2+ channels. Our results may provide a cellular and molecular mechanism that helps explain the physiological functions of orexin in the brain.

11.
Front Chem ; 10: 1035691, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36300027

RESUMO

The lithium-air (Li-air) battery utilizes infinite oxygen in the air to store or release energy through a semi-open cathode structure and bears an ultra-high theoretical energy density of more than 1,000 Wh/kg. Therefore, it has been denoted as the candidate for next-generation energy storage in versatile fields such as electric vehicles, telecommunications, and special power supply. Among all types of Li-air batteries, an aqueous Li-air battery bears the advantages of a high theoretical energy density of more than 1,700 Wh/kg and does not have the critical pure oxygen atmosphere issues in a non-aqueous lithium-air battery system, which is more promising for the actual application. To date, great achievements have been made in materials' design and cell configurations, but critical challenges still remain in the field of the solid electrolyte separator, its related lithium stripping/plating at the lithium anode, and catholyte design. In this mini-review, we summarized recent progress related to the solid electrolyte in aqueous Li-air batteries focusing on both material and battery device development. Moreover, we proposed a discussion and unique outlook on improving solid electrolyte compatibility and battery performance, thus designing an aqueous Li-air battery with higher energy density and better cycle performance in the future.

12.
Anal Methods ; 14(41): 4085-4094, 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36200386

RESUMO

In REE:NaY(WO4)2 laser crystals, optical properties like laser conversion efficiency are dependent on the doped rare earth element (REE) concentration, which necessitates the importance for accurate determination of the REE concentration in these precious samples. However, in situ microanalysis of these samples by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) is often hampered by the lack of matrix-matched reference materials. In this work, a REE-doped NaY(WO4)2 single crystal (NaYW-500) that has a nominal REE concentration of 500 µg g-1 was synthesized and employed as a candidate reference material. Its homogeneity (1 RSD of elemental concentration or 89Y-normalized signal intensity) was measured by electron probe microanalysis (EPMA) and LA-ICP-MS to be less than 2% for major elements and mainly <3% for REEs, respectively. Then, an LA-ICP-MS analytical method was developed by using 89Y as the internal standard and using NaYW-500 as the external calibrator under the optimal operating conditions. Quantitative determination of the REE concentration in the other two REE:NaY(WO4)2 single crystals NaYW-50 and NaYW-5000 show that these samples can be accurately measured with relative deviations (Dr) of -6.00 to 12.33% and -9.86 to 6.94%, respectively. Further application of the proposed analytical method to quantitative determination of the Ho concentration in a Ho:NaY(WO4)2 laser crystal shows that desirable accuracy was obtained with a Dr of 4.62%. It demonstrates that the proposed method by preparing REE-doped NaY(WO4)2 single crystals for quantitative determination of the REE concentration in NaY(WO4)2 laser crystals is valid and robust.

13.
Front Digit Health ; 4: 1007784, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36274654

RESUMO

We are rapidly approaching a future in which cancer patient digital twins will reach their potential to predict cancer prevention, diagnosis, and treatment in individual patients. This will be realized based on advances in high performance computing, computational modeling, and an expanding repertoire of observational data across multiple scales and modalities. In 2020, the US National Cancer Institute, and the US Department of Energy, through a trans-disciplinary research community at the intersection of advanced computing and cancer research, initiated team science collaborative projects to explore the development and implementation of predictive Cancer Patient Digital Twins. Several diverse pilot projects were launched to provide key insights into important features of this emerging landscape and to determine the requirements for the development and adoption of cancer patient digital twins. Projects included exploring approaches to using a large cohort of digital twins to perform deep phenotyping and plan treatments at the individual level, prototyping self-learning digital twin platforms, using adaptive digital twin approaches to monitor treatment response and resistance, developing methods to integrate and fuse data and observations across multiple scales, and personalizing treatment based on cancer type. Collectively these efforts have yielded increased insights into the opportunities and challenges facing cancer patient digital twin approaches and helped define a path forward. Given the rapidly growing interest in patient digital twins, this manuscript provides a valuable early progress report of several CPDT pilot projects commenced in common, their overall aims, early progress, lessons learned and future directions that will increasingly involve the broader research community.

14.
Opt Express ; 30(21): 38503-38512, 2022 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-36258414

RESUMO

Graphene has unique advantages in ultrabroadband detection. However, nowadays graphene-based photodetectors cannot meet the requirements for practical applications due to their poor performance. Here, we report a graphene-silicon-graphene Schottky junction photodetector assisted by field effect. Two separate graphene sheets are located on both sides of the n-doped silicon to form two opposite lateral series heterojunctions with silicon, and a transparent top gate is designed to modulate the Schottky barrier. Low doping concentration of silicon and negative gate bias can significantly raise the barrier height. Under the combined action of these two measures, the barrier height increases from 0.39 eV to 0.77 eV. Accordingly, the performance of the photodetector has been greatly improved. The photoresponsivity of the optimized device is 2.6 A/W at 792 nm, 1.8 A/W at 1064 nm, and 0.42 A/W at 1550 nm, and the on/off photo-switching ratio reaches 104. Our work provides a feasible solution for the development of graphene-based optoelectronic devices.

15.
Risk Anal ; 2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36307897

RESUMO

As the decision-making basis for "safety" in risk management and risk assessment activities, acceptable risk has always been an important topic of risk-related research. Based on the records in the Science Citation Index Expanded database and Social Sciences Citation Index database via the Web of Science Core Collection, 1124 articles or reviews related to acceptable risk in engineering and operations research and management science were retrieved. These documents, published between 1961 and 2021, covered 3056 authors, 75 countries/territories, 1296 institutions, and 323 journals. In this study, bibliometric data such as annual growth trends were analyzed by using descriptive statistics. Through the co-occurrence maps created by CiteSpace and Gephi, the most productive and influential countries/territories, institutions, and authors as well as their cooperation networks were identified. Further analysis was conducted to determine the core publications and publication sources in this field through co-citation analysis. Insights into focus areas and research topics over time were obtained through keyword co-occurrence analysis. This study provides a macroscopic overview of acceptable risk research and may help researchers better understand this research field and predict its dynamic directions.

16.
Clin Transl Oncol ; 2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36309619

RESUMO

PURPOSE: Lung cancer (LC) is the most common malignancy in the world. It is well that hypoxia is common in lung cancer, which contributes to lung cancer progression and metastasis [1]. miRNA-27a as a repressor factor is a lowly expression within non-small cell lung cancer (NSCLC). However, the molecular mechanism between miR-27a and hypoxia in lung cancer progression remains poorly understood. This study aims to explore hypoxia promotes epithelial-mesenchymal transition in lung cancer cells via regulating the NRF2/miR­27a/BUB1 pathway. METHODS: We detect the expression of miR-27a after exposure to hypoxia conditions in lung cancer cells via qPCR. Using MTT assay and colony assay to assess the ability of proliferation in lung cancer cells under hypoxia or transfect miR-27a mimics. The capability of migration and invasion was evaluated by wound healing assay and Boyden-chamber assay. The mRNA and protein expression of EMT markers was respectively detected by qPCR and western blot. We detected NRF2 occupancy at the miR-27a promoter by ChIP-Seq analysis. Meanwhile, the luciferase assay verified BUB1 as a direct target of miR-27a. RESULTS: We found hypoxia promotes lung cancer cell proliferation, migration, invasion, and the epithelial-mesenchymal transition (EMT) process by inhibiting the miR-27a expression. miR-27a mimics significantly reduced the promotion effect of hypoxia on the invasion and proliferation of lung cancer cells. NRF2 as regulating the oxidation/anti-oxidation factor was activated under hypoxia conditions. The activation of NRF2 repressed miR-27a expression. On the contrary, the inhibitory effect of hypoxia on miR-27a was reversed when the NFE2L2 gene was silenced. Ectopic expression of NRF2 inhibited miR-27a expression under normoxia. We further validated BUB1 as a direct target of the miR-27a by luciferase assay. CONCLUSION: Hypoxia promotes invasion and epithelial-mesenchymal transition of Lung cancer cells by regulating the NRF2/miR-27a/BUB1 axis.

17.
Artigo em Inglês | MEDLINE | ID: mdl-36272006

RESUMO

Natural nanoparticles (NNP) are ubiquitous in natural water and can interact with other contaminants, causing ecotoxic effects on aquatic nontarget organisms. However, the impact of NNPs on the ecotoxicity of antibiotics remains largely unknown. This work investigated the acute toxicity, chronic effect, and oxidative response and damage in Daphnia magna co-exposed to phenicol antibiotics (chloramphenicol, thiamphenicol) and different concentrations of NNPs (10 mg/L: environmentally relevant concentration; 100 mg/L: a high concentration that caused no apparent immobilization in D. magna). The results showed that the acute toxicity of chloramphenicol was increased by 10 mg/L NNPs but decreased by 100 mg/L NNPs; both concentrations of NNPs increased and decreased acute toxicities of thiamphenicol and chloramphenicol + thiamphenicol treatments, respectively. After long-term exposure, phenicol antibiotics (1 µg/L) and NNP (10 mg/L) mixtures in environmentally relevant concentrations significantly affected the reproduction of D. magna but did not influence their growth. The catalase activity, reduced glutathione level, and malonaldehyde content in D. magna also varied with the NNPs concentrations. Notably, the lowest concentration of thiamphenicol and chloramphenicol + thiamphenicol combined with NNPs significantly increased the malondialdehyde content in D. magna compared with the control, indicating membrane lipid peroxidation occurred in daphnids. This study suggests that the toxic effects of contaminants and NNPs on aquatic organisms should be considered thoroughly to avoid underestimating the hazard of these pollutants in the actual aquatic environment.

18.
J Am Chem Soc ; 144(36): 16292-16297, 2022 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-36054904

RESUMO

A bioinspired synthesis of spirochensilide A from commercially available lanosterol is reported. The synthesis features a directed C-H oxidation, a Wagner-Meerwein-type double methyl migration, a Meinwald rearrangement, and a double-bond isomerization/spiroketal formation cascade. The proposed biosynthetic speculation was modified by this synthetic sequence, which also served as a platform for the synthesis of other lanostanes with migrating methyl groups.


Assuntos
Lanosterol , Triterpenos , Lanosterol/química , Oxirredução
19.
Acta Biomater ; 152: 425-439, 2022 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-36113723

RESUMO

Engineering smart nano-therapeutics for re-epithelialisation of chronic wounds facilitates the wound healing process. However, due to excessive oxidative stress damage and persistent inflammation in diabetic wound microenvironment, the migration of stimulating epidermal cells in diabetic wounds represents a significant challenge. Here we synthesised P311-loaded micelles by self-assembly of P311 peptides and diblock copolymer poly (ethylene glycol)-block-poly (propylene sulfide) (PEG-b-PPS, denoted as PEPS) that have unique ability to transform an oxidative wound microenvironment into a proregenerative one while also providing cues for epidermal cell migration. The P311@PEPS showed an accelerated migration of epidermal cells via activation of the Akt signalling pathway, simultaneously suppressing the unfavourable oxidative wound microenvironment by scavenging reactive oxygen species (ROS), ultimately leading to the induction of an environment conducive to cell migration. Furthermore, the micelles were able to bypass the inhibitory effect of ROS on the Akt signalling pathway, thereby promoting epidermal cell migration. Additionally, we observed that diabetic wounds treated with P311@PEPS showed accelerated chronic wound healing, granulation tissue formation, collagen deposition and re-epithelialisation, thereby suggesting the efficacy of P311@PEPS as a promising nanoplatform for the treatment of chronic wounds. STATEMENT OF SIGNIFICANCE: Based on the unique conditions of the diabetic wound microenvironment, a smart drug delivery system with ROS-responsive nanomaterials has been widely investigated to enhance diabetic wound healing. In our previous studies, we observed that P311 promotes epidermal cell migration to induce wound re-epithelialisation. However, the application of P311 suffers from its instability. Herein, we developed a therapeutic platform with P311-loaded micelles (P311@PEPS), which were synthesized by the self-assembly of P311 peptides and diblock copolymer poly (ethylene glycol)-block-poly (propylene sulfide) (PEG-b-PPS, denoted as PEPS). These micelles provide continuous migration signals for epidermal cells by ROS-trigged P311 release. Additionally, P311@PEPS scavenges excess ROS and provides a microenvironment that reduces inflammation, which could protect P311 from enzymatic degradation and improve the bioavailability of P311.


Assuntos
Diabetes Mellitus , Reepitelização , Colágeno/metabolismo , Humanos , Inflamação , Micelas , Estresse Oxidativo , Peptídeos/metabolismo , Peptídeos/farmacologia , Polietilenoglicóis/metabolismo , Polímeros/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Sulfetos
20.
Head Neck ; 44(11): 2417-2427, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35916453

RESUMO

BACKGROUND: The potential role of circFNDC3B in regulating oral tongue squamous cell carcinoma development (OTSCC) remains unknown. METHODS: The level of circFNDC3B in OTSCC tissues or cell lines was measured and its function in vitro and in vivo was analyzed. Interactions among circFNDC3B, miR-1322, and MED1 were verified by luciferase reporter and RNA pull-down assays. RESULTS: The level of circFNDC3B in tissues or cell lines of OTSCC was higher than that in control groups. siRNA-mediated circFNDC3B inhibition resulted in weakened proliferation, migration, and invasion, which was reversed by miR-1322. Overexpression of MED1 in OTSCC cells partially reversed the tumor suppression functions of si-circFNDC3B or miR-1322 mimics in vitro. circFNDC3B overexpression dramatically promoted tumor growth in vivo. circFNDC3B directly bound with miR-1322 and consequently promoted the MED1 expression in OTSCC cells. CONCLUSIONS: The circFNDC3B/miR-1322/MED1 axis participates in OTSCC progression, which may provide novel therapeutic targets for OTSCC.


Assuntos
MicroRNAs , RNA Circular , Carcinoma de Células Escamosas de Cabeça e Pescoço , Neoplasias da Língua , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Fibronectinas/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Subunidade 1 do Complexo Mediador/genética , Subunidade 1 do Complexo Mediador/metabolismo , MicroRNAs/genética , RNA Circular/genética , RNA Interferente Pequeno , Carcinoma de Células Escamosas de Cabeça e Pescoço/genética , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Neoplasias da Língua/genética , Neoplasias da Língua/patologia
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