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1.
Environ Geochem Health ; 41(1): 297-308, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29948539

RESUMO

Using sodium alginate hydrogel as skeleton, in combination with chitosan and magnetic Fe3O4, a new type of magnetic chitosan/sodium alginate gel bead (MCSB) was prepared. Adsorptive removal of Cu(II) from aqueous solutions was studied by using the MCSB as a promising candidate in environmental application. Different kinetics and isotherm models were employed to investigate the adsorption process. Based on Fourier transform infrared spectroscopy, field-emission scanning electron microscope, CHNS/O elements analysis, vibration magnetometer, and various means of characterization, a comprehensive analysis of the adsorption mechanism was conducted. The MCSB had a good magnetic performance with a saturation magnetization of 12.5 emu/g. Elemental analysis proved that the addition of chitosan introduced a considerable amount of nitrogen-rich groups, contributing significantly to copper adsorption onto gel beads. The contact time necessary for adsorption was optimized at 120 min to achieve equilibrium. Experimental data showed that the adsorption process agreed well with the Langmuir isotherm model and the pseudo-second-order kinetics model. The theoretical maximum adsorption capacity of MCSB for Cu(II) could reach as high as 124.53 mg/g. In conclusion, the MCSB in this study is a novel and promising composite adsorbent, which can be applied for practical applications in due course.


Assuntos
Alginatos/química , Quitosana/química , Cobre/isolamento & purificação , Nanosferas/química , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Adsorção , Concentração de Íons de Hidrogênio , Cinética , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/ultraestrutura , Nanosferas/ultraestrutura , Termodinâmica
2.
Cancer Cell Int ; 18: 40, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29568235

RESUMO

Background: Low expression of E2F3a and caspase 8 associated protein 2 (CASP8AP2) are associated with poor prognosis of childhood acute lymphoblastic leukemia (ALL). Methods: Dual-luciferase reporter assay and wild type as well as four mutated types of reporter plasmids were used to demonstrate the activation of E2F3a on CASP8AP2 transcription. The direct binding of E2F3a with the promoter of CASP8AP2 was shown by Chromatin Immunoprecipitation (ChIP). Cell proliferation activity and cell cycle were determined by MTS and flow cytometry in leukemic cells after treating with common chemotherapeutic drugs vincristine and daunorubicin. Results: In this study, we found that up-regulation of E2F3a in leukemic cells led to increased fraction of cells in S and G2/M phase, accelerated proliferation, and enhanced sensitivity to vincristine and daunorubicin. ChIP and luciferase assay indicated that E2F3a could directly bind to two fragments in the wild type of CASP8AP2 promotor (- 206 to - 69 and - 677 to - 507), and activate its transcription activity which was reduced in mutated promotors. The effect of E2F3a on chemotherapeutic sensitivity of leukemic cells could be reversed by down-regulating CASP8AP2. Conclusions: E2F3a could promote transcription and expression of CASP8AP2. The effect of E2F3a on chemotherapeutic sensitivity of ALL cells was implemented by regulating CASP8AP2 expression to a great extent.

3.
AIDS Res Hum Retroviruses ; 32(9): 892-4, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27118612

RESUMO

Polymorphisms of the hepatitis C virus (HCV) core protein and NS3 have been described to be associated with liver cirrhosis (LC) and/or hepatocellular carcinoma in patients with chronic hepatitis C genotype 1b (HCV 1b). Here, we determine whether there is an association between LC and polymorphisms of viral core protein and NS3 in patients in Hubei province, China. A total of 42 patients with chronic HCV 1b (21 patients with LC and 21 with chronic hepatitis [CH]) were enrolled, amino acid sequence of the core protein and N-terminus of NS3 were obtained by direct sequencing and compared with the prototype strain HCV-J. No significant difference of amino acid polymorphisms was observed between isolates from LC and CH patients in the core protein. However, in the N-terminus of NS3, amino acid polymorphisms at positions A1072T (Ala 1072 chronic Thr 1072 cirrhosis), I1074V (Ile 1074 chronic Val 1074 cirrhosis), and T1098N/I (Thr 1098 chronic Asn or Ile 1098 cirrhosis) correlated significantly with LC. These findings indicate that the polymorphisms of HCV at these sites may be the risk factors for the development of LC in patients with chronic HCV 1b.


Assuntos
Hepacivirus/classificação , Hepacivirus/genética , Hepatite C Crônica/complicações , Hepatite C Crônica/virologia , Cirrose Hepática/virologia , Polimorfismo Genético , Proteínas não Estruturais Virais/genética , China , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Sequência de DNA , Proteínas do Core Viral/genética
4.
Ying Yong Sheng Tai Xue Bao ; 27(7): 2197-2204, 2016 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-29737127

RESUMO

With secondary forest in the montane region of eastern Liaoning Province as research object, this paper analyzed the spatial distribution and scale effect of Gleason richness index, Simpson dominance index, Shannon diversity index and Pielou evenness index in a 4 hm2 plot. The results showed that spatial distributions of the four diversity indices showed higher spatial heterogeneity. Variance of the four diversity indices varied with increasing scale. Coefficients of variation of the four diversity indices decreased with increasing scale. The four diversity indices of the tree layer were higher than those of the shrub layer, and the variation tendency varied with increasing scale. The results indicated that sampling scale should be taken into account when studying species diversity in the montane region of eastern Liaoning Province.


Assuntos
Biodiversidade , Florestas , China , Dispersão Vegetal , Análise Espacial , Árvores
5.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 44(4): 578-83, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-24059112

RESUMO

OBJECTIVE: To evaluate the correlation between image quality and thoracic diameters, anteroposterior diameter (AP) and right-left diameter (RL) for the optimization of reducing radiation dose in coronary artery imaging with Dual-source CT. METHODS: 118 patients underwent CT coronary artery imaging (CTCA) were divided into two groups (A and B) according to body mass index (BMI). There were 58 patients in group A with BMI values > or = 25 kg/m2 and < 30 kg/m2; and 60 patients in group B with BMI values < 25 kg/m2. Scan parameters: collimation 64 X 0.6 mm, reconstruction slice thickness 0.75 mm, reconstructin interval 0.7 mm, FOV 150-180 mm, the pitch adjusted according to heart rate automatically. CT imagings were obtained with tube voltage 100 kV, tube current 330 mAs (group A) and 100 kV, tube current 220 mAs (group B). The contrast-noise ratio (CNR), signal to noise ratio (SNR), as well as blind correlation test score values were calculated to establish the relationship among BMI, AP, RL and CNR, SNR. RESULTS: RL of five levels in groups of A, B were correlated to CNRa (P < 0.05). In group A and B (except iii, iv level of Group A), AP were associated with CNRa (P < 0.05). In addition to ii, iv level of group A, v level of group B, BMI were associated with CNRa (P < 0.05). The correlation coefficient of CNRa and RL of group A except ii level was greater than that of CNRa and AP. The correlation coefficient of CNRa, SNRa and RL of group B was greater than that of CNRa, SNRa and AP. Except iii level, the correlation coefficient of CNRa, SNRa and AP of group A was greater than that of CNRa, SNRa and BMI. Except ii level, the correlation coefficient of CNRa and AP of group B was greater than that of CNRa and BMI. CONCLUSION: Thoracic diameter of RL can be used as a guider to select the appropriate scan protocol in the coronary imaging acqusition with dual-source CT.


Assuntos
Angiografia Coronária , Doses de Radiação , Intensificação de Imagem Radiográfica , Tórax/anatomia & histologia , Tomografia Computadorizada Espiral , Adulto , Idoso , Idoso de 80 Anos ou mais , Carga Corporal (Radioterapia) , Índice de Massa Corporal , Doença da Artéria Coronariana/complicações , Doença da Artéria Coronariana/diagnóstico por imagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
6.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(4): 588-91, 596, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22997902

RESUMO

OBJECTIVE: To investigate the value of split-bolus 2-phase with virtual non-enhanced scan in CT urography. METHODS: Eighty (80) patients with urinary obstruction were divided into two groups, group A was traditional CT urography scan, group B was split-bolus 2-phase with virtual non-enhanced scan. The scan sequence of group A included conventional scan first, then enhanced scan in arterial phase, parenchyma phase and excretion delayed phase after the injection of 100 mL contrast agent. The scan sequence of group B included conventional scan and enhanced scan following two bolus injection of contrast; the first injection of contrast was 40 mL, the second injection was 60 mL with 15-30 minutes delay, then enhanced scan was conducted in arterial and parenchymal phase, the parenchymal phase scan was performed with dual energy scanning sequence. The image quality of urinary collecting system, the detection of urinary obstructive lesion, and radiation dose were compared between the two groups. RESULTS: Between the two imaging methods, the imaging quality of urinary system did not show significant difference, but radiation dose was statistically significant difference. The radiation dose of split-bolus 2-phase with virtual non-enhanced scan was only 55% of traditional CTU scan. CONCLUSION: Split-bolus2 phase with virtual non-enhanced scan could obtain the same image quality as tranditional CTU, while reducing the approximately half the radiation dose.


Assuntos
Doses de Radiação , Tomografia Computadorizada Espiral/métodos , Cálculos Urinários/diagnóstico por imagem , Urografia/métodos , Adulto , Idoso , Meios de Contraste , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Interpretação de Imagem Radiográfica Assistida por Computador , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Obstrução Ureteral/diagnóstico por imagem , Doenças Urológicas/diagnóstico por imagem , Adulto Jovem
7.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(4): 592-6, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22997903

RESUMO

OBJECTIVE: To evaluate the clinical value of low-dose chest pain protocol for cardiac and chest vascular imaging using dual-source computed tomography. METHODS: 102 patients with requests for examinations of cardiac and thoracic blood vessels, were scanned in chest pain protocol for angiography. With heart electrical impulses dose control techniques, maximum tube current within the R-R interval was set up by different heart rate. Patients with a heart rate less than or equal to 75 beats/min were divided into two groups: regular-dose group (A) 28 patients; low-dose group (B) 25 patients. Patient with a heart rate of 76-120 beats/min were also divided into two groups: regular-dose group (C) 24 patients; low-dose group (D) 25 patients. The image quality of pulmonary artery, coronary artery and aortic were blind evaluated. The effective dose for each patient was calculated and group comparisons were made. RESULTS: Every radiation dose gained satisfactory images. No differences in image quality were found between Group A and Group B, and between Group C and Group D (P > 0.05). The effective doses of Group A (7.09 +/- 0.95) mSv were significantly higher than those of Group B (5.01 +/- 0.34) mSv. The effective doses of Group C (4.79 +/- 0.91) mSv were significantly higher than those of Group D (2.48 +/- 0.43) mSv. CONCLUSION; For patients with acute chest pain and relatively stable heart rate, their cardiac and thoracic vessels can be shown clearly in images of low-dose chest pain protocol, which provides a rapid and clear diagnostic instrument.


Assuntos
Dor no Peito/diagnóstico por imagem , Angiografia Coronária/métodos , Doses de Radiação , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Tomografia Computadorizada por Raios X/métodos , Adulto , Idoso , Angiografia Coronária/instrumentação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Artéria Pulmonar/diagnóstico por imagem , Interpretação de Imagem Radiográfica Assistida por Computador
8.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 43(4): 597-600, 2012 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-22997904

RESUMO

OBJECTIVE: To investigate the clinical value of dual-source computed tomography dual-energy Iodine overlay technique in the imaging diagnosis of acute necrotizing pancreatitis. METHODS: The imaging data were retrospectively analyzed in 67 cases of acute necrotizing pancreatitis underwent contrast-enhanced dual-source dual-energy CT in portal venous phase. The CT imaging parameters, including the difference of CT value between pancreatic parenchyma and necrotic lesion, contrast-to-noise ratio of pancreatic parenchyma-to-necrosis, area of pancreatic necrosis and score of subjective diagnosis, were measured and assessed on CT images of 80 kV, 140 kV, weighted-average 120 kV as well as Iodine overlay. RESULTS: The differences of CT value between pancreatic parenchyma and necrosis in the images of 80 kV, 140 kV, weighted-average 120 kV and Iodine overlay were (67.40 +/- 20.82) HU, (42.87 +/- 14.99) HU, (48.69 +/- 15.82) HU, (33.01 +/- 10.26) HU, respectively; contrast-to-noise ratios of pancreatic parenchyma-to-necrosis of each group were 8.36 +/- 3.58, 5.85 +/- 2.65, 7.68 +/- 3.51, 10.60 4.34; area of pancreatic necrosis of each group was (3.78 +/- 2.68) cm2, (3.28 +/- 2.59) cm2, (3.37 +/- 2.46) cm2, (2.42 +/- 1.98) cm2; the score of subjective diagnosis of each group was 3.88 +/- 0.33, 3.31 +/- 0.80, 3.58 +/- 0.66, 2.81 +/- 0.76, respectively. The four indexes in the images of Iodine overlay were significantly different from those of another three groups (P < 0.05). Contrast-to-noise ratio of pancreatic parenchyma-to-necrosis in the images of Iodine overlay was significantly higher than that of another three groups, while the difference of CT value, area of pancreatic necrosis and score of subjective diagnosis were lower. CONCLUSION; Dual-source CT dual-energy Iodine overlay is not helpful to improve subjective judgment in the diagnosis of pancreatic necrosis, but contributes to the display of hypoperfusion area around the necrosis.


Assuntos
Meios de Contraste , Pancreatite Necrosante Aguda/diagnóstico por imagem , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Tomografia Computadorizada por Raios X/métodos , Ácidos Tri-Iodobenzoicos , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
9.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 42(5): 691-4, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22007500

RESUMO

OBJECTIVE: To investigate the clinical value of dual-source computed tomography dual-energy technology in the imaging diagnosis of acute necrotizing pancreatitis. METHODS: Thirty-two cases with diagnosed acute necrotizing pancreatitis and underwent contrast-enhanced dual-source dual-energy CT in portal venous phase were retrospectively analyzed. Difference of CT value between pancreatic parenchyma and necrosis, contrast to noise ratio of pancreatic parenchyma-to-necrosis and score of subjective diagnosis were measured, calculated and assessed on CT images of 80 kV, 140 kV and weighted-average 120 kV. RESULTS: The difference of CT value between pancreatic parenchyma and necrosis in the images of 80 kV, 140 kV and weighted-average 120 kV was (67.22 +/- 21.11) HU, (42.91 +/- 15.12) HU, (48.78 +/- 16.13) HU; contrast to noise ratio of pancreatic parenchyma-to-necrosis in the images of each group was 8.35 +/- 3.63, 5.89 +/- 2.67, 7.72 +/- 3.58; the score of subjective diagnosis in the images of each group was 3.87 +/- 0.34, 3.28 +/- 0.81, 3.56 +/- 0.67 respectively. Difference of CT value between pancreatic parenchyma and necrosis, contrast to noise ratio of pancreatic parenchyma-to-necrosis and score of subjective diagnosis were significantly different in three groups of images (P < 0.05). The three indexes at 80 kV were significantly higher than those of 140 kV and weighted-average 120 kV. CONCLUSION: At portal venous phase dual-sourcedual-energy enhancement CT, the demonstration of pancreas necrosis is better than those at 80 kV, 140 kV and weighted-average 120 kV in the diagnosis of acute necrotizing pancreatitis.


Assuntos
Pancreatite Necrosante Aguda/diagnóstico por imagem , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Tomografia Computadorizada Espiral/métodos , Adulto , Idoso , Meios de Contraste/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Intensificação de Imagem Radiográfica/métodos , Estudos Retrospectivos
10.
J Nanosci Nanotechnol ; 11(11): 9705-8, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22413276

RESUMO

Y2O3 acts as the matrix material when doped with different content of La2O3 for reducing sintering temperature and refining grains. The (Nd(0.01)La(x)Y(0.99-x))2O3 nanoparticles and transparent ceramics are fabricated by a combustion synthesis. The powder feature is characterized by TEM. The microstructure, mechanical properties and transmittance of the samples are examined by SEM, HV-1000 hardness tester and fluorescence analyzer respectively. The results show that the (Nd(0.01)La(x)Y(0.99-x))2O3 nanoparticles are homogeneous in size and nearly spherical with average diameter in the range of 40-60 nm. There are no other phases except the Y2O3 cubic phase in the (Nd(0.01)La(x)Y(0.99-x))2O3 nanoparticles. The grains of the samples significantly reduce with increasing La2O3 content. The hardness and fracture toughness increase rapidly first and then gradually tend to plateau with increasing La2O3 content. The transmittance of sample also increases gradually with increasing La2O3, the largest transmittance exceeds 77% when the La2O3 content is x = 0.12.

11.
Zhonghua Gan Zang Bing Za Zhi ; 18(9): 689-93, 2010 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-20943082

RESUMO

OBJECTIVE: To investigate the incidence of hepatotoxicity in acquired immunodeficiency syndrome (AIDS) patients on combined anti-retroviral therapy (cART) containing nevirapine (NVP) and to assess the risk factors and its impact on cART. METHODS: 330 AIDS patients from March 2003 to June 2008 at local county were enrolled and a retrospective study using Kaplan-meier survival and Multivariate logistic regression modeling was conducted. RESULTS: 267 out of 330 patients received NVP based cART and 63 cases received EFV-based cART. The deference of prevalences of hepatotoxicity between the two groups is statistically significant (Chi2 = 6.691, P = 0.01). 133 out of 267 (49.8%) patients on NVP based cART had at least one episode of ALT elevation during a median 21 months (interquartile ranges, IQR 6, 37) follow-up time, amounts for 28.5 cases per 100 person-years. Baseline ALT elevation (OR = 14.368, P = 0.017)and HCV co-infection (OR = 3.009, P = 0.000) were risk factors for cART related hepatotoxicity, while greatly increased CD4+ T(CD4) cell count was protective against hepatotoxicity development (OR = 0.996, P = 0.000). Patients co-infected with HCV received NVP-based cART had the higher probability of hepatotoxicity than those without HCV co-infection (Log rank: Chi2 = 16.764, P = 0.000). 23 out of the 133 subjects (17.3%) with NVP related hepatotoxicity discontinued cART temporarily or shifted NVP to efavirenz. CONCLUSION: NVP related hepatotoxicity was common among ARV naive HIV infected subjects in our cohort. Baseline ALT elevation and HCV co-infection were associated statistically with the development of hepatotoxicity. Hepatotoxicity led to discontinuing cART temporarily or switching to other regimens in some subjects. It suggested that NVP should be used with caution in patients co-infected with HCV among whom anti-HCV therapy before cART initiation may contribute to minimizing the probability of NVP associated hepatotoxicity.


Assuntos
Síndrome de Imunodeficiência Adquirida/metabolismo , Antirretrovirais/efeitos adversos , Doença Hepática Induzida por Substâncias e Drogas/epidemiologia , Fígado/efeitos dos fármacos , Nevirapina/efeitos adversos , Síndrome de Imunodeficiência Adquirida/tratamento farmacológico , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático , Doença Hepática Induzida por Substâncias e Drogas/virologia , Feminino , Humanos , Incidência , Fígado/metabolismo , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Adulto Jovem
13.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 41(1): 170-3, 181, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20369497

RESUMO

OBJECTIVE: To evaluate the accuracy of using 64-MDCT to assess right ventricular function. METHODS: The ventricular function of 32 adult volunteers were assessed by ECG-gated 64-MDCT and MRI, which included right ventricular end-diastolic and end-systolic volume, stroke volume, and ejection fraction. Consistency between the two modalities was tested with Bland and Altman analysis and Pearson's correlation. RESULTS: No significant differences were found in calculated RV volumes and EF between the two modalities (P = 0.29 to 0.66). Consistency was achieved in RV-EDV [(0.6 +/- 4.9) mL; r = 0.98)], RV-ESV [(-0.6 +/- 3.4) mL; r = 0.97], RV-SVC [(0.9 +/- 5.6) mL; r = 0.93, and RV-EFC (1.0 +/- 3.9) mL; r = 0.88]. CONCLUSION: ECG-gated 64-MDCT can accurately assess RV function.


Assuntos
Tomografia Computadorizada Espiral/métodos , Função Ventricular Direita/fisiologia , Adulto , Eletrocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
14.
J Nanosci Nanotechnol ; 10(3): 1924-7, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20355601

RESUMO

Y2O3 nanoparticles doped with different concentrations of Nd3+ were prepared by the coprecipitation method, and the Nd3+:Y2O3 transparent ceramics were fabricated by vacuum sintering at 1700 degrees C, under 1 x 10(-3) Pa for 6 h. The structural, morphological, and luminescence properties of the samples were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and fluorescence analysis. The results show that the Nd3+:Y2O3 nanoparticles are homogeneous in size and nearly spherical, and the average diameter of the particles is in the range of 40-60 nm. There are no other phases except the Y2O3 cubic phase in the Nd3+:Y2O3 nanoparticles and transparent ceramics. Nd3+ dissolves completely in the Y2O3 cubic phase. The relative density of Nd3+:Y2O3 transparent ceramics is 99.7%, and the transmittance of the Nd3+:Y2O3 transparent ceramics exceeds 80% at the wavelength range.

15.
World J Gastroenterol ; 15(8): 996-1003, 2009 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-19248201

RESUMO

AIM: To analyze the influence of human immunodeficiency virus (HIV) infection on the course of hepatitis C virus (HCV) infection. METHODS: We performed a meta-analysis to quantify the effect of HIV co-infection on progressive liver disease in patients with HCV infection. Published studies in the English or Chinese-language medical literature involving cohorts of HIV-negative and -positive patients coinfected with HCV were obtained by searching the PUBMED, EMBASE and CBM. Data were extracted independently from relevant studies by 2 investigators and used in a fixed-effect meta analysis to determine the difference in the course of HCV infection in the 2 groups. RESULTS: Twenty-nine trails involving 16750 patients were identified including the outcome of histological fibrosis or cirrhosis or de-compensated liver disease or hepatocellular carcinoma or death. These studies yielded a combined adjusted odds ratio (OR) of 3.40 [95% confidence interval (CI) = 2.45 and 4.73]. Of note, studies that examined histological fibrosis/cirrhosis, decompensated liver disease, hepatocellular carcinoma or death had a pooled OR of 1.47 (95% CI = 1.27 and 1.70), 5.45 (95% CI = 2.54 and 11.71), 0.76 (95% CI = 0.50 and 1.14), and 3.60 (95% CI = 3.12 and 4.15), respectively. CONCLUSION: Without highly active antiretroviral therapies (HAART), HIV accelerates HCV disease progression, including death, histological fibrosis/cirrhosis and decompensated liver disease. However, the rate of hepatocellular carcinoma is similar in persons who had HCV infection and were positive for HIV or negative for HIV.


Assuntos
Infecções por HIV/complicações , Hepatite C/complicações , Hepatite C/fisiopatologia , Carcinoma Hepatocelular/epidemiologia , Progressão da Doença , Infecções por HIV/mortalidade , Hepatite C/mortalidade , Humanos , Neoplasias Hepáticas/epidemiologia , Seleção de Pacientes
16.
Zhonghua Yi Xue Za Zhi ; 89(36): 2583-5, 2009 Sep 29.
Artigo em Chinês | MEDLINE | ID: mdl-20137625

RESUMO

OBJECTIVE: To observe the effects of different concentrations of glucose and insulin in cell differentiation from bone marrow stem cells (BMSC) into osteoblasts (OB) and explore the acting mechanism of glucose and insulin in bone metabolism. METHODS: The in vitro technique of cell culture was employed to separate, purify and cultivate BMSC from the tibia and femur of rats. Then the BMSCs were differentiated into osteoblasts under the inductions of different glucose concentrations (5.6, 25, 50 mmol/L) with or without the addition of insulin (0.6 microg/ml). Later the calcium nodule cell ratio of osteoblasts in alizarin red stain was observed microscopically to assay the mRNA expression of OB markers-ALP, BGP and Runx2 by real-time PCR. RESULTS: With the increasing glucose concentrations, the number of red calcium nodules obviously decreased. PCR showed that the mRNA expression of ALP, BGP and transcription factor Runx2 decreased too. The difference was significant (P < 0.05). The differentiation of OB decreased with with the increasing glucose concentrations. The cell number of red calcium nodules obviously increased in the same glucose concentration groups. The mRNA expression of ALP, BGP and Runx2 also increased (P < 0.01). It hinted that insulin could enhance the differentiation of OB. CONCLUSION: Glucose can inhibit the differentiation from BMSC into OB. The inhibition is more sensitive along with the concentration increments. This may be one of the pathogenesises of diabetic osteoporosis. Insulin can enhance the differentiation from BMSC into OB. In addition, it can improve the inhibition of differentiation from BMSC into OB because of a high concentration of glucose.


Assuntos
Células da Medula Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Glucose/farmacologia , Insulina/farmacologia , Osteoblastos/efeitos dos fármacos , Animais , Células da Medula Óssea/citologia , Proliferação de Células , Células Cultivadas , Células-Tronco Hematopoéticas , Masculino , Osteoblastos/citologia , Ratos , Ratos Wistar
17.
Guang Pu Xue Yu Guang Pu Fen Xi ; 28(10): 2254-7, 2008 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19123383

RESUMO

Y2O3 acted as the matrix material, which was doped with different concentrations of Er3+, Er3+ : Y2O3 nanocrystalline powder was prepared by co-precipitation method, and Er3+ : Y2O3 transparent ceramics was fabricated by vacuum sintering at 1700 degrees C, 1 x 10(-3) Pa for 8 h. By using the X-ray diffraction (D/MAX-RB), transmission electron microscopy(Philips EM420), automatic logging spectrophotometer(DMR-22), fluorescence analyzer (F-4500) and 980 nm diode laser, the structural, morphological and luminescence properties of the sample were investigated. The results show that Er3+ dissolved completely in the Y2O3 cubic phase, the precursor was amorphous, weak diffraction peaks appeared after calcination at 400 degrees C, and if calcined at 700 degrees C, the precursor turned to pure cubic phase. With increasing the calcining temperature, the diffraction peaks became sharp quickly, and when the calcining temperature reached 1100 degrees C, the diffraction peaks became very sharp, indicating that the grains were very large. The particles of Er+ : Y2O3 is homogeneous and nearly spherical, the average diameter of the particles is in the range of 40-60 nm after being calcined at 1000 degrees C for 2 h. The relative density of Er3+ : Y2O3 transparent ceramics is 99.8%, the transmittance of the Er2+ : Y2O3 transparent ceramics is markedly lower than the single crystal at the short wavelength, but the transmittance is improved noticeably with increasing the wavelength, and the transmittance exceeds 60% at the wavelength of 1200 nm. Excited under the 980 nm diode laser, there are two main up-conversion emission bands, green emission centers at 562 nm and red emission centers at 660 nm, which correspond to (4)S(3/2) / (2)H(11/2) - (4)I(15/2) and (4)F(9/2) - (4)I(15/2) radiative transitions respectively. By changing the doping concentrations of Er3+, the color of up-conversion luminescence can be tuned from green to red gradually. The luminescence intensity is not reinforce with the increase in the concentration, so the doping concentration of Er3+ should not exceed 2%. If the doping concentration of Er3+ exceeds the range, the concentration has very small effect on the improvement of luminescence intensity.

18.
Zhonghua Nei Ke Za Zhi ; 45(9): 721-4, 2006 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-17166444

RESUMO

OBJECTIVE: The purpose of this study was to analyze the chest CT findings of immunocompetent patients with primary pulmonary cryptococcosis and to evaluate the utility of CT-guided percutaneous biopsy in the diagnosis. METHODS: Chest CT scans of 12 immunocompetent patients with biopsy-proven primary pulmonary cryptococcosis were analyzed for the number of lesions, morphologic characteristics, distribution of parenchymal abnormalities, and the presence of lymphadenopathy and pleural effusion. Lung specimens were obtained by CT guided percutaneous biopsy (n = 9) and/or surgical resection (n = 7). A pulmonary pathologist reviewed the specimens. RESULTS: The main manifestations were classified into two patterns. Localized multiple mixed lesions (nodules and/or masses and/or consolidation) were found in 67% (8/12) of the cases, and single nodules were found in 33% (4/12). Associated findings included air bronchograms (n = 9), cavity (n = 2), and CT halo sign (n = 4). Lung specimens were obtained by CT guided percutaneous biopsy in 9 cases, of which 7 (78%) were confirmed by pathology. Seven cases recovered after treatment with fluconazole, but cryptococcal meningitis occurred in 1 case 5 month after single excision. CONCLUSIONS: Chest CT findings of primary pulmonary cryptococcosis in immunocompetent patients have a predominant pattern of localized multiple mixed lesions. CT guided percutaneous biopsy is helpful in confirming the diagnosis. Fluconazole is effective in the treatment of pulmonary cryptococcosis in these patients.


Assuntos
Criptococose/diagnóstico , Pneumopatias Fúngicas/diagnóstico , Biópsia , Criptococose/diagnóstico por imagem , Humanos , Pneumopatias Fúngicas/diagnóstico por imagem , Tomografia Computadorizada por Raios X
19.
Zhonghua Yi Xue Za Zhi ; 84(22): 1876-8, 2004 Nov 17.
Artigo em Chinês | MEDLINE | ID: mdl-15631797

RESUMO

OBJECTIVE: To evaluate the efficacy and safety of cefdinir in the treatment of patients with mild to moderate bacterial community acquired pneumonia (CAP). METHODS: A prospective single-blind randomized controlled clinical study was performed comparing cefdinir with cefaclor in the treatment of sixty-four patients with CAP. The clinical and bacteriological efficacy and safety were compared between cefdinir and cefaclor in treating mild to moderate CAP. Thirty-three patients were treated with cefdinir 100 mg, orally, three times a day (cefdinir group), thirty-one patients were treated with cefaclor 500 mg, orally, three times a day (cefaclor group). In both groups 7-14 d was a treatment course. RESULTS: The cure rate of cefdinir and cefaclor was 84.8% and 77.4% respectively and the overall efficacy rate was 93.9% and 87.1% respectively. The bacterial positive rates and bacterial eradication rates of the two groups were 81.8%, 80.7% and 96.3%, 88%, respectively. The adverse drug reaction rate were 3% in cefdinir group and 6.5% in cefaclor group. There was no statistical significant difference between the two groups for the above results (P >0.05). The time of given medicine of cefdinir and cefaclor was (10.8 +/- 1.6) d and (12.1 +/- 1.7) d (P <0.01) respectively. CONCLUSION: cefdinir is safe and effective, shorten the course of treatment in the treatment of mild to moderate bacterial community acquired pneumonia.


Assuntos
Antibacterianos/uso terapêutico , Cefaclor/uso terapêutico , Cefalosporinas/uso terapêutico , Infecções Comunitárias Adquiridas/tratamento farmacológico , Pneumonia Bacteriana/tratamento farmacológico , Adolescente , Adulto , Idoso , Cefdinir , Infecções Comunitárias Adquiridas/microbiologia , Humanos , Pessoa de Meia-Idade , Pneumonia Bacteriana/microbiologia , Estudos Prospectivos , Método Simples-Cego , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estreptocócicas/tratamento farmacológico
20.
Zhonghua Jie He He Hu Xi Za Zhi ; 26(4): 223-6, 2003 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-12901830

RESUMO

OBJECTIVE: To describe the radiographic and CT findings of primary pulmonary non-Hodgkin lymphoma (PPL),and to evaluate percutanous transthoracic needle biopsy (PTNB) in the diagnosis of PPL. METHODS: Chest radiographs and CT scans of three patients with histologically proven PPL were reviewed. The diagnosis of PPL was confirmed histologically with specimens obtained by means of PTNB. RESULTS: Consolidation with air bronchograms on the chest X-ray films and CT scans was found in all cases. Multiple ill-defined nodules of various size in both lungs and a mass with air bronchogram in the right lower lobe on the CT scan were present in one case. Ground-glass opacity or reticular lesions were present in both lung fields. Hilar/mediastinal adenopathy and pericardial/pleural effusion were not features of these cases. PTNB was diagnostic in all three cases. No complications occurred during these procedures. CONCLUSIONS: PPL has some specific imaging features. Radiographic and CT findings are helpful in the diagnosis of primary pulmonary malignant lymphoma. A specific diagnosis can be obtained by means of PTNB.


Assuntos
Neoplasias Pulmonares/diagnóstico por imagem , Linfoma não Hodgkin/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Adulto , Idoso , Diagnóstico Diferencial , Feminino , Humanos , Neoplasias Pulmonares/patologia , Linfoma não Hodgkin/patologia , Masculino , Pessoa de Meia-Idade , Radiografia Torácica , Nódulo Pulmonar Solitário/diagnóstico por imagem
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