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1.
Pharmacogn Mag ; 12(45): 50-6, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27019561

RESUMO

BACKGROUND: Raw Moutan Cortex (RMC), derived from the root bark of Paeonia suffruticosa, and Processed Moutan Cortex (PMC) is obtained from RMC by undergoing a stir-frying process. Both of them are indicated for different pharmacodynamic action in traditional Chinese medicine, and they have been used in China and other Asian countries for thousands of years. OBJECTIVE: To establish a method to study the RMC and PMC, revealing their different chemical composition by fingerprint, qualitative, and quantitative ways. MATERIALS AND METHODS: High-performance liquid chromatography coupled with diode array detector and electrospray mass spectrometry (HPLC-DAD-ESIMS) were used for the analysis. Therefore, the analytes were separated on an Ultimate TM XB-C18 analytical column (250 mm × 4.6 mm, 5.0 µm) with a gradient elution program by a mobile phase consisting of acetonitrile and 0.1% (v/v) formic acid water solution. The flow rate, injection volume, detection wavelength, and column temperature were set at 1.0 mL/min, 10 µL, 254 nm, and 30°C, respectively. Besides, principal components analysis and the test of significance were applied in data analysis. RESULTS: The results clearly showed a significant difference among RMC and PMC, indicating the significant changes in their chemical compositions before and after the stir-frying process. CONCLUSION: The HPLC-DAD-ESIMS coupled with chemometrics analysis could be used for comprehensive quality evaluation of raw and processed Moutan Cortex. SUMMARY: The experiment study the RMC and PMC by HPLC-DAD-ESIMS couple with chemometrics analysis. The results of their fingerprints, qualitative, and quantitative all clearly showed significant changes in their chemical compositions before and after stir-frying processed. Abbreviation used: HPLC-DAD-ESIMS: High-performance Liquid Chromatography-Diode Array Detector-Electrospray Mass Spectrometry, RMC: Raw moutan cortex, PMC: Processed moutan cortex, TCM: Traditional Chinese medicine, PCA: Principal components analysis, LOD: Limit of detection, LOQ: Limit of quantitation, RSD: Relative standard deviation.

2.
Zhong Yao Cai ; 38(1): 25-8, 2015 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26214866

RESUMO

OBJECTIVE: To develop a method for the determination of aldoses in edible bird' s nest (EBN) from Southeast Asia. METHODS: Firstly, the sample was hydrolyzed by 2 mol/L HCl-methanol, neutralized by 2 mol/L potassium hydroxide methanol solution, and acetylated by acetic anhydride in pyridine. Then, four aldoses were separated completely with HP-5 capillary chromatogram column,by using FID tester, through programmed temperature. RESULTS: The EBN's GC spectrum was significantly different from its adulterants. CONCLUSION: The GC method is characteristic, simple and reliable, which can be used to control the quality of EBN.


Assuntos
Aves , Cromatografia Gasosa , Monossacarídeos/química , Animais , Ásia Sudeste
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