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1.
J Virol Methods ; 300: 114387, 2021 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-34848281

RESUMO

The H1N1 subtype influenza viruses (H1N1) have been causing persistent epidemics in human, swine and poultry populations since 1918. This subtype has evolved into four relatively stable genetic lineages, including classical swine influenza virus lineage, seasonal human influenza virus lineage, avian influenza virus lineage and Eurasian avian-like swine influenza virus lineage. In this study, four pairs of primers, based on the relatively conserved HA nucleotide regions of each H1N1 genetic lineage, were designed to establish a SYBR Green-based real-time quantitative RT-PCR (qPCR) assay to differentiate between the H1N1 genetic lineages. The results of qPCR assay showed that the lineage-specific primers designed for each H1N1 lineage were intra-lineage-specific, without mismatch of inter-lineage or inter-subtype and there appeared specific amplification curves when the concentrations of H1N1 plasmids were greater than or equal to 1.0 × 101 copies/reaction. Thus, this qPCR assay can specifically differentiate between the four lineages of H1N1 with a good specificity and sensitivity, which would assist in recognizing the infection and epidemic status of different H1N1 genetic lineages.

2.
ACS Nano ; 2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34860017

RESUMO

In order to overcome the shuttling effect of soluble polysulfides in lithium-sulfur (Li-S) batteries, we have designed and synthesized a creative MoS2-MoO3/carbon shell (MoS2-MoO3/CS) composite by a H2O2-enabled oxidizing process under mild conditions, which is further used for separator modification. The MoS2-MoO3 heterostructures can conform to the CS morphology, forming two-dimensional nanosheets, and thus shorten the transport path of lithium ion and electrons. Based on our theoretical calculations and experiments, the heterostructures show strong surface affinity toward polysulfides and good catalytic activity to accelerate polysulfide conversion. Benefiting from the above merits, the Li-S battery with a MoS2-MoO3/CS modified separator exhibits good electrochemical performance: it delivers a high discharge capacity of 1531 mAh g-1 at 0.2 C; the initial capacity can be maintained by 92% after 600 cycles at 1 C, and the discharge capacity decay rate is only 0.0135% per cycle. Moreover, the MoS2-MoO3/CS battery still achieves good cycling stability with 78% capacity retention after 100 cycles at 0.2 C with a high sulfur loading of 5.9 mg cm-2. This work offers a facile design to construct the MoS2-MoO3 heterostructures for high-performance Li-S batteries, and may also improve one's understanding on the heterostructure contribution during polysulfide adsorption and conversion.

3.
Cancer Manag Res ; 13: 8169-8178, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34754237

RESUMO

Purpose: To analyze the effects of radiotherapy and its timing on the survival and safety of patients with newly diagnosed distant metastatic NPC in non-high-incidence areas. Patients and Methods: We retrospectively analyzed 94 newly diagnosed NPC patients with distant metastatic admitted to our hospital from January 2011 to June 2018. They were divided into three groups: no radiotherapy group received chemotherapy alone, early radiotherapy group was combined with radiotherapy during 1 to 3 cycles of chemotherapy, and late radiotherapy group was combined with radiotherapy after 4-6 cycles of chemotherapy were effective. The efficacy and side effects of the three groups were compared, and the prognostic factors were analyzed. Results: The 6-month, 1-year and 2-year PFS were 53.6%, 14.3% and 3.6% in no radiotherapy group, 71.0%, 38.7% and 19.4% in early radiotherapy group, 88.6%, 48.6% and 22.9% in late radiotherapy group; the radiotherapy groups were better than the no radiotherapy group, and the difference was statistically significant (P < 0.017). The 1-year, 2-year and 3-year OS were 75.0%, 32.1% and 0 in no radiotherapy group, 77.4%, 54.8% and 12.9% in early radiotherapy group, 85.7%, 71.4% and 31.4% in late radiotherapy group; the radiotherapy groups were better than the no radiotherapy group, and the differences were statistically significant (P < 0.017). There was no significant difference in OS and PFS between the two radiotherapy groups. Univariate and multivariate analysis showed that HBV (P = 0.031), number of metastases (P = 0.002), liver metastases (P = 0.038), radiotherapy (P < 0.001) and treatment response (P = 0.011) were related to OS. There was no significant difference in the incidence of adverse events (P > 0.017). Conclusion: Early and late combined radiotherapy had similar clinical efficacy and both prolonged PFS and OS for patients with newly diagnosed distant metastatic NPC in non-high-risk areas. If chemotherapy response is expected to be poor, radiotherapy can be received early.

4.
Can J Microbiol ; : 1-12, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34644507

RESUMO

Brucella abortus is a gram-negative intracellular parasite bacteria that causes serious health hazards in humans and animals. The type IV secretion system (T4SS), encoded by the virB promoter, has been identified as an important virulence factor for Brucella abortus, but its impact on Brucella abortus A19 remains unclear. In this study, the T4SS of Brucella abortus A19 was inactivated by deletion of the virB promoter, resulting in a mutant strain A19ΔvirB. Real-time PCR and western blotting analysis demonstrated that T4SS-related proteins were not expressed after virB promoter deletion. Moreover, the survival rate of A19 in high-salt and strong acidic environments decreased after virB promoter deletion. Compared to the parental strain A19, the A19ΔvirB mutant strain showed reduced growth rate in TSB, decreased invasion ability to macrophages and dendritic cells, and reduced virulence of the mutant strain in macrophages, dendritic cells, and mice. In addition, the A19ΔvirB mutant strain showed enhanced autophagy in macrophages and dendritic cells compared with A19, and the A19ΔvirB mutant strain was able to upregulate IL-6 and downregulate IL-10 in macrophages. These data help us to better understand the T4SS of the A19 vaccine strain and contribute to our efforts to improve Brucella vaccines.

5.
Exp Mol Med ; 53(8): 1207-1217, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34385569

RESUMO

Compelling evidence has indicated the vital role of lysine-specific demethylase 4 A (KDM4A), hypoxia-inducible factor-1α (HIF1α) and the mechanistic target of rapamycin (mTOR) signaling pathway in nasopharyngeal carcinoma (NPC). Therefore, we aimed to investigate whether KDM4A affects NPC progression by regulating the HIF1α/DDIT4/mTOR signaling pathway. First, NPC and adjacent tissue samples were collected, and KDM4A protein expression was examined by immunohistochemistry. Then, the interactions among KDM4A, HIF1α and DDIT4 were assessed. Gain- and loss-of-function approaches were used to alter KDM4A, HIF1α and DDIT4 expression in NPC cells. The mechanism of KDM4A in NPC was evaluated both in vivo and in vitro via RT-qPCR, Western blot analysis, MTT assay, Transwell assay, flow cytometry and tumor formation experiments. KDM4A, HIF1α, and DDIT4 were highly expressed in NPC tissues and cells. Mechanistically, KDM4A inhibited the enrichment of histone H3 lysine 9 trimethylation (H3K9me3) in the HIF1α promoter region and thus inhibited the methylation of HIF1α to promote HIF1α expression, thus upregulating DDIT4 and activating the mTOR signaling pathway. Overexpression of KDM4A, HIF1α, or DDIT4 or activation of the mTOR signaling pathway promoted SUNE1 cell proliferation, migration, and invasion but inhibited apoptosis. KDM4A silencing blocked the mTOR signaling pathway by inhibiting the HIF1α/DDIT4 axis to inhibit the growth of SUNE1 cells in vivo. Collectively, KDM4A silencing could inhibit NPC progression by blocking the activation of the HIF1α/DDIT4/mTOR signaling pathway by increasing H3K9me3, highlighting a promising therapeutic target for NPC.

6.
Eur J Med Chem ; 221: 113488, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-33991963

RESUMO

Naturally occurring cyclic antimicrobial peptides (AMPs) such as tyrocidine A (Tyrc A) and gramicidin S (GS) are appealing targets for the development of novel antibiotics. However, their therapeutic potentials are limited by undesired hemolytic activity and relatively poor activity against Gram-negative bacteria. Inspired by polycationic lipopeptide polymyxin B (PMB), the so called 'last-resort' antibiotic for the treatment of infections caused by multidrug-resistant Gram-negative bacteria, we synthesized and biologically evaluated a series of polycationic analogues derived from Tyrc A. We were able to obtain peptide 8 that possesses 5 positive charges exhibiting potent activities against both Gram-negative and Gram-positive bacteria along with totally diminished hemolytic activity. Intriguingly, antibacterial mechanism studies revealed that, rather than the 'pore forming' model that possessed by Tyrc A, peptide 8 likely diffuses membrane in a 'detergent-like' manner. Furthermore, when treating mice with peritonitis-sepsis, peptide 8 showed excellent antibacterial and anti-inflammatory activities in vivo.


Assuntos
Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Polimixina B/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Tirocidina/farmacologia , Antibacterianos/síntese química , Antibacterianos/química , Linhagem Celular , Relação Dose-Resposta a Droga , Farmacorresistência Bacteriana/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Polimixina B/química , Relação Estrutura-Atividade , Tirocidina/síntese química , Tirocidina/química
7.
BMC Infect Dis ; 21(1): 460, 2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34016047

RESUMO

BACKGROUND: This case report describes the clinical process of a shepherd who suffered brucellosis-related endocarditis (BE) and spondylitis (BS) and was infected with Brucella melitensis biovar 3 (B. melitensis biovar 3). CASE PRESENTATION: A 55-year-old male patient was admitted to The First Affiliated Hospital of Shihezi University on October 11, 2018, due to over 3 months of intermittent fever, back pain, and heart trouble. The Rose Bengal Plate test was positive, the standard agglutination test titer for brucellosis was 1/800, and the blood culture was positive for B. melitensis biovar 3. Three instances of transthoracic echocardiography examination at days 1, 25, and 376 after admission to the hospital and magnetic resonance imaging (MRI) and computed tomography (CT) checks at days 5 and 38 revealed that the size of the vegetation on the posterior leaflet of the mitral valve increased from 0.7 × 1.4 cm to 1.2 × 1.5 cm and that the left atrium and ventricle were enlarged. The MRI and CT results showed hyperplasia of the second and third vertebra, a cold abscess formed on both sides of the psoas major muscles, and the vertebra hyperplasia became aggravated at a later time point. The patient's situation deteriorated, and heart failure was discovered on October 22, 2019. At the moment of submission of this manuscript, the patient remains in bed at home because of severe debility caused by brucellosis. CONCLUSIONS: This is the first reported case of endocarditis combined with spondylitis caused by B. melitensis biovar 3 in a shepherd. Brucellosis infection can cause work-power losses because of misdiagnosis or a lack of proper treatment. Early diagnosis and treatment are essential for a successful outcome.


Assuntos
Brucella melitensis , Brucelose/microbiologia , Endocardite Bacteriana/microbiologia , Espondilite/microbiologia , Testes de Aglutinação , Brucelose/diagnóstico , Brucelose/patologia , Endocardite Bacteriana/diagnóstico , Endocardite Bacteriana/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Valva Mitral/microbiologia , Valva Mitral/patologia , Espondilite/diagnóstico
8.
Front Vet Sci ; 8: 642968, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33816587

RESUMO

Exosomes are small extracellular vesicles that are released from cells and that function in intercellular communication. Recently, interferon-inducible transmembrane protein 3 (IFITM3) has been identified as a highly effective anti-intracellular pathogen protein that can inhibit the invasion of a wide range of pathogenic microorganisms. However, whether Brucella infection induces secretion of exosomes and whether these exosomes contain IFITM3 protein remain unknown. Here, we focused on the immune function of extracellular IFITM3 protein in the process of Brucella infection. This study is the first to show that Brucella melitensis strain M5 (Brucella M5) can stimulate macrophages to secrete large amounts of exosomes. Most importantly, we identified exosomes from Brucella M5-infected cells that were rich in molecules of IFITM3, and these exosomes could transmit the IFITM3 from one cell to another, thereby effectively inhibiting the intracellular survival of Brucella. Moreover, immunization with exosomes carrying IFITM3 decreased mouse spleen tissue damage and spleen colony forming unit (CFU), leading to the establishment of an anti-Brucella state in mice. In conclusion, our findings provide new insights into the anti-Brucella mechanism of IFITM3-containg exosomes, thus providing a theoretical foundation for systematic elaboration of the mechanisms of Brucella infection and host immunity. The results provide new ideas for the development of candidate vaccines for Brucella.

9.
Nanotechnology ; 32(27)2021 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-33765671

RESUMO

Lithium-sulfur batteries (LSBs) have gained intense research enthusiasm due to their high energy density. Nevertheless, the 'shuttle effect' of soluble polysulfide (a discharge product) reduces their cycling stability and capacity, thus restricting their practical application. To tackle this challenging issue, we herein report a sulfonated covalent organic framework modified separator (SCOF-Celgard) that alleviates the shuttling of polysulfide anions and accelerates the migration of Li+ions. Specifically, the negatively charged sulfonate can inhibit the same charged polysulfide anion through electrostatic repulsion, thereby improving the cycle stability of the battery and preventing the Li-anode from being corroded. Meanwhile, the sulfonate groups may facilitate the positively charged lithium ions to pass through the separator. Consequently, the battery assembled with the SCOF-Celgard separator exhibits an 81.1% capacity retention after 120 cycles at 0.5 C, which is far superior to that (55.7%) of the battery with a Celgard separator. It has a low capacity degradation of 0.067% per cycle after 600 cycles at 1 C, and a high discharge capacity (576 mAh g-1) even at 2 C. Our work proves that the modification of a separator with a SCOF is a viable and effective route for enhancing the electrochemical performance of a LSB.

11.
Bioresour Technol ; 325: 124731, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33486412

RESUMO

During the mining of rare earth mineral, the use of lanthanum-containing fertilizers, and the disposal of lanthanum-containing electronic products, the content of lanthanum (La(III)) in typical ammonia wastewater with low carbon to nitrogen ratio is increasing day by day. Here, effects of La(III) on anammox process in performance, microbial community structure, metabolic function, and microbial co-occurrence network were investigated. The results shown that the nitrogen removal efficiency was declines briefly and then gradually recovers after low dosage (1-5 mg/L) La(III) treatment and the decrease to low level (24.25 ± 1.74%) under high La(III) dosage (10 mg/L). La(III) in the range of 1-5 mg/L significantly promoted the relative abundance of Anammoxoglobus (0.024% to 9.762%). The blocking of key metabolic pathways was confirmed to cause the breakdown of anammox by PICRUSt. Furthermore, network analysis revealed that lack of cooperation bacteria limits the activity of Anammoxoglobus.


Assuntos
Desnitrificação , Microbiota , Reatores Biológicos , Lantânio , Nitrogênio , Oxirredução , Águas Residuárias
12.
Ecotoxicol Environ Saf ; 209: 111796, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33341697

RESUMO

Cerium Ce(III) is one of the major pollutants contained in wastewater generated during Ce(III) mining. However, the effect(s) of Ce(III) on the functional genera responsible for removing nitrogen biologically from wastewater has not been studied and reported. In this study, the effects of Ce(III) on aspects of partial-nitritation-(PN) process including ammonia oxidation rate (AOR), process kinetics, and microbial activities were investigated. It was found that the effect of dosing Ce(III) in the PN system correlated strongly with the AOR. Compared to the control, batch assays dosed with 5 mg/L Ce(III) showed elevated PN efficiency of about 121%, an indication that maximum biological response was feasible upon Ce(III) dose. It was also found that, PN performance was not adversely affected, given that Ce(III) dose was ≤20 mg/L. Process kinetics investigated also suggested that the maximum Ce(III) dose without any visible inhibition to the activities of ammonium oxidizing bacteria was 1.37 mg/L, but demonstrated otherwise when Ce(III) dose exceeded 5.63 mg/L. Compared to the control, microbes conducted efficient Ce(III) removal (averaged 98.66%) via biosorption using extracellular polymeric substances (EPS). Notably, significant deposits of Ce(III) was found within the EPS produced as revealed by SEM, EDX, CLSM and FTIR. 2-dimensional correlation infrared-(2DCOS-IR) revealed ester group (uronic acid) as a major organic functional group that promoted Ce(III) removal. Excitation-emission matrix-(EEM) spectrum and 2DCOS-IR suggested the dominance of Fulvic acid, hypothesized to have promoted the performance of the PN process under Ce(III) dosage.


Assuntos
Cério/toxicidade , Nitrificação/efeitos dos fármacos , Águas Residuárias/química , Poluentes Químicos da Água/toxicidade , Compostos de Amônio , Bactérias , Reatores Biológicos/microbiologia , Mineração , Nitrogênio , Oxirredução , Esgotos , Águas Residuárias/microbiologia
13.
Innate Immun ; 27(1): 81-88, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33241964

RESUMO

Brucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. Brucella survival and replication inside immune cells is critical for the establishment of chronic infections. Protein modifications by small ubiquitin-related modifier proteins and the NF-κB pathway are involved in many cellular activities, playing major roles in regulating protein function that is essential for pathogenic bacteria during infection. However, the relationship between them in the intracellular survival of Brucella is still largely unknown. We demonstrated that Brucella abortus 2308 infection can activate the expression of small ubiquitin-related modifier-2 proteins in a time-dependent manner. We found the production of Th1 cytokines (IFN-γ and TNF-α) and the transcription of NF-κB/p65 were promoted by overexpression and inhibited by interference of small ubiquitin-related modifier-2. In addition, we showed that small ubiquitin-related modifier-2 can inhibit intracellular survival of Brucella abortus 2308 by regulating activation of the NF-κB pathway. Taken together, this work shows that small ubiquitin-related modifier-2 modification of NF-κB2/p65 is essential for the survival of Brucella abortus 2308 inside macrophages. This work may help to unravel the pathogenic mechanisms of Brucella infections.

14.
Front Microbiol ; 11: 599205, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33281799

RESUMO

To date, a variety of Brucella effector proteins have been found to mediate host cell secretion, autophagy, inflammation, and other signal pathways, but nuclear effector proteins have not yet been reported. We identified the first Brucella nucleomodulin, BspJ, and we screened out the BspJ interaction host proteins NME/NM23 nucleoside diphosphate kinase 2 (NME2) and creatine kinase B (CKB) through yeast two-hybrid and co-immunoprecipitation assays. These proteins are related to the host cell energy synthesis, metabolism, and apoptosis pathways. Brucella nucleomodulin BspJ will decrease the expression level of NME2 and CKB. In addition, BspJ gene deletion strains promoted the apoptosis of macrophages and reduced the intracellular survival of Brucella in host cells. In short, we found nucleomodulin BspJ may directly or indirectly regulate host cell apoptosis through the interaction with NME2 and CKB by mediating energy metabolism pathways in response to the intracellular circulation of Brucella infection, but the mechanism needs further study.

15.
Am J Cancer Res ; 10(10): 3157-3178, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33163263

RESUMO

Hepatocellular carcinoma (HCC) patients always have a background of cirrhosis. Aberrant epigenetic changes in cirrhosis provide a conductive environment for HCC tumorigenesis. Active enhancers (AEs) are essential for epigenetic regulation and play an important role in cell development and the progression of many diseases. However, the role of AEs in the progression from cirrhosis to HCC remains unclear. We systemically constructed a landscape of AEs that developed de novo in cirrhosis and were conserved in HCC, referred to as CL-HCC AEs. We observed significant upregulation of these CL-HCC AE-associated genes in cirrhosis and HCC, with no other epigenetic changes. Enrichment analysis of these CL-HCC AE-associated genes revealed enrichment in both hepatocyte-intrinsic tumorigenesis and tumor immune response, which might contribute to HCC tumorigenesis. Analysis of the diagnostic ability of these CL-HCC AE-associated genes provided a five-gene (THBS4, OLFML2B, CDKN3, GABRE, and HDAC11) diagnostic biomarker for HCC. Molecular subtype (MS) identification based on the CL-HCC AE-associated genes identified 3 MSs. Samples representing the 3 MSs showed differences in CL-HCC AE-associated gene expression levels, prognosis, copy number variation (CNV)/mutation frequencies, functional pathways, tumor microenvironment (TME) cell subtypes, immunotherapy responses and putative drug responses. We also found that the BET bromodomain inhibitor JQ1 downregulated the expression of CL-HCC AE-associated genes. Collectively, our results suggest that CL-HCC AEs and their associated genes contribute to HCC tumorigenesis and evolution, and could be used to distinguish the different landscapes of HCC and help explore the mechanism, classification, prediction, and precision therapy of HCC.

16.
Medicine (Baltimore) ; 99(45): e23065, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33157964

RESUMO

Dental general anesthesia (DGA) is a safe and high-quality restorative and preventive treatment option for children with severe early childhood caries (S-ECC), who require extensive dental treatment and exhibit anxiety and emotional or cognitive immaturity or are medically compromised. However, several postoperative complications have been reported in children under DGA. This study aimed to evaluate and analyze the prevalence of the relevant factors of postoperative complications in healthy Chinese children following DGA to provide a foundation for pre-, intra-, and postoperative overall health management for healthy and disabled children after DGA.A total of 369 systematically healthy Chinese children (36-71 months old) undergoing a DGA were studied. Data were collected on patients' histories, characteristics, anesthesia, and dental procedures. Parents or caregivers were interviewed before and 72 hours after the procedure. Data were analyzed using logistic regression.Approximately 94.86% of the enrolled children reported one or more complications. The most prevalent complication was postoperative pain (62.70%), followed by weariness, agitation, masticatory problems, drowsiness, oral bleeding, coughing, fever, sore throat, nausea, constipation, epistaxis, vomiting, excitement, and diarrhea. The long duration of the operation was a risk factor for postoperative pain and weariness. A high nutritional status could be a protective factor for postoperative fever.Prolonged operation means complex treatment, such as pulp therapy or extraction. We speculate that the longer the duration is, the more difficult the dental procedures are. The accumulation of discomfort leads to pain. We suspect that children in lower nutritional levels are more likely to suffer from bacteremia or dehydration, resulting in fever.Postoperative pain was the most prevalent complication after the DGA. A decrease in dental procedure duration might reduce the odds of postoperative pain and weariness. A high nutritional status could be a protective factor for postoperative fever. Children with low nutritional status could be more susceptible to postoperative fever.


Assuntos
Anestesia Dentária/efeitos adversos , Anestesia Geral/métodos , Cárie Dentária/cirurgia , Procedimentos Cirúrgicos Bucais/efeitos adversos , Complicações Pós-Operatórias/epidemiologia , Anestesia Geral/estatística & dados numéricos , Ansiedade/epidemiologia , Estudos de Casos e Controles , Pré-Escolar , Estudos Transversais , Feminino , Humanos , Masculino , Estado Nutricional , Dor Pós-Operatória/epidemiologia , Prevalência , Estudos Prospectivos , Segurança
17.
PLoS One ; 15(10): e0232568, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33125372

RESUMO

We studied livestock abortion and various associated risk factors in the Ili region of northwest China. Livestock abortion prevalence was estimated and correlated with infections (Brucellosis, Salmonellosis, Mycoplasma and Chlamydia seropositivity) and management (farming type and contact with other herds/flocks) risk factors. A total of 2996 serum samples (1406 cow, 1590 sheep) were identified by RBPT (Rose Bengal Plate Test) and c-ELISA (competitive-enzyme linked immunosorbent assay), and they showed the overall seroprevalence of brucellosis in the study area was cow 6.76%, sheep 9.50%. The seroprevalence of brucellosis in X county was cow 7.06%, sheep 9.12%; in H county was cow 11.70%, sheep 10.80%; and in Q county was cow 4.22%, sheep 9.11%. The overall seroprevalence of Mycoplasma in the study area was cow 3.20%, sheep 6.42%. The seroprevalence of Mycoplasma in X county was cow 3.39%, sheep 7.98%; in H county was cow 5.26%, sheep 9.97%; and in Q county was cow 2.11%, sheep 4.33%. The Odds ratio of brucellosis for cow and sheep, respectively, were 45.909 [95% CI 26.912-78.317, P<0.001] and 70.507 [95% CI 43.783-113.544, P<0.001] times higher than other abortion-related factors including mixed farming, contact with other flocks and Mycoplasma infection. A total of 54 samples, including aborted cow (22), sheep (30) fetuses and milk samples (2), were identified as Brucella melitensis (B. melitensis) positive. A total of 38 Brucella were isolated from 16 aborted cow, 20 sheep fetuses and 2 milk samples. All of these isolates were identified, and confirmed, as B. melitensis. A phylogenetic tree showed that the Brucella isolates closely matched the B. melitensis biovar 3 isolated in Inner Mongolia, China, and B. melitensis isolated from Norway and India. These results suggest that B. melitensis biovar 3 is the main pathogen responsible for cow and sheep abortion and also pose a human health risk. Additionally, livestock reproduction can also be influenced by Mycoplasma infection and managerial factors (farming type and contact with other herds/flocks), especially in remote areas.


Assuntos
Aborto Animal/epidemiologia , Aborto Animal/etiologia , Aborto Animal/microbiologia , Criação de Animais Domésticos , Animais , Brucella melitensis , Brucelose/epidemiologia , Brucelose/veterinária , Brucelose Bovina/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , China/epidemiologia , Chlamydia , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/veterinária , Feminino , Humanos , Infecções por Mycoplasma/epidemiologia , Infecções por Mycoplasma/veterinária , Gravidez , Prevalência , Fatores de Risco , Salmonelose Animal/epidemiologia , Ovinos , Doenças dos Ovinos/epidemiologia , Carneiro Doméstico
18.
Arch Virol ; 165(11): 2453-2459, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32767108

RESUMO

Canine parvovirus type 2 (CPV-2) is currently circulating in domestic and wild animals, but our knowledge about CPV-2 infections in raccoon dogs is limited. In this study, VP2 gene sequences of CPV-2 were amplified from rectal swabs of 14 diarrhetic raccoon dogs (Nyctereutes procyonoides) in Hebei province, China, in 2016 and 2017. Phylogenetic analysis of the VP2 gene sequences revealed that most of these sequences (11 of 14) belonged to the same subclade as raccoon dog strain CPV-2/Raccoon_Dog/China/DP-1/16 isolated from Shandong province in 2016. A comparison of deduced amino acid sequences revealed presence of the substitutions S297A and S27T in 11 of those 14 sequences. I418T was observed in a minority of the sequences (4 of 14). In addition, A300D and T301I, P13S and I219V, and N419K were found in three of the sequences. This study shows that CPV-2 strains with different substitutions in their VP2 amino acid sequences were spreading among raccoon dogs in Hebei during 2016 and 2017 and suggests that further studies are needed to monitor the distribution of these strains in China.


Assuntos
Infecções por Parvoviridae/veterinária , Parvovirus Canino/classificação , Cães Guaxinins/virologia , Sequência de Aminoácidos , Animais , Proteínas do Capsídeo/genética , China/epidemiologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/virologia , Parvovirus Canino/isolamento & purificação
19.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 38(3): 284-289, 2020 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-32573136

RESUMO

OBJECTIVE: This study aimed to evaluate the postoperative complications undergoing dental general anesthesia in children and analyze the prevalence and related factors. METHODS: This prospective study involved 292 systematically healthy children (36 to 71 months old) who received extensive dental treatment under general anesthesia. Data about patients' histories, characteristics, dental and anesthesia procedure were collected. Parents or caregivers were interviewed face to face preoperation and 72 h postoperation. Data were analyzed using logistic regression. RESULTS: Approximately 93.5% of the enrolled children reported one or more complications. The most prevalent complication was postoperative pain, followed by weariness, agitation, problem in eating, drowsiness, oral bleeding, cough, fever, etc. The length of operative time and femininity were the risks of the postoperative pain. Nutrition status was the factor probably in association with fever. CONCLUSIONS: The children receive longer operative time and girls show to be more susceptible to the postoperative pain. High nutrition status could be the protective factor of postoperative fever.


Assuntos
Anestesia Dentária , Cárie Dentária , Anestesia Geral , Criança , Pré-Escolar , Assistência Odontológica , Feminino , Humanos , Complicações Pós-Operatórias , Estudos Prospectivos
20.
Can J Microbiol ; 66(5): 351-358, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32040345

RESUMO

Iron is a fundamental element required by most organisms, including Brucella. Several researchers have suggested that the iron response regulator (irr) and rhizobial iron regulator (rirA) genes regulate iron acquisition by Brucella abortus, influencing heme synthesis by and virulence of this pathogen. However, little is known about another Brucella species, Brucella melitensis. In this research, we successfully constructed two mutants: M5-90Δirr and M5-90ΔrirA. The adhesion, invasion, and intracellular survivability of these two mutants were evaluated in RAW264.7 cells infected with 1 × 106 CFU of M5-90Δirr, M5-90ΔrirA, or M5-90. We also tested the sensitivity of cells to hydrogen peroxide and their ability to grow. In addition, the virulence of these two mutants was evaluated in BALB/c mice. The results showed that the ability of these two mutants to invade and adhere inside the murine macrophages RAW264.7 was attenuated but their ability to replicate intracellularly was strengthened, enhancing the resistance to hydrogen peroxide. The M5-90Δirr mutant showed stronger growth ability than the parental strain under iron-limiting conditions. No differences were observed in the number of bacteria in spleen between M5-90 and M5-90Δirr at 7 or 15 days postinfection. However, the number of M5-90ΔrirA in spleen reduced significantly at 15 days postinfection. The splenic index of the M5-90Δirr group is evidently lower than that of M5-90. This is the first report that irr and rirA genes of B. melitensis are associated not only with virulence but also with growth ability. Together, our data suggest that M5-90Δirr is a promising Brucella vaccine candidate.


Assuntos
Proteínas de Bactérias/genética , Brucella melitensis/genética , Brucella melitensis/patogenicidade , Regulação Bacteriana da Expressão Gênica/fisiologia , Ferro/metabolismo , Fatores de Transcrição/genética , Animais , Anti-Infecciosos Locais/toxicidade , Western Blotting , Vacina contra Brucelose/imunologia , Brucelose/prevenção & controle , Contagem de Colônia Microbiana , Feminino , Peróxido de Hidrogênio/toxicidade , Macrófagos/microbiologia , Camundongos , Camundongos Endogâmicos BALB C , Baço/microbiologia , Virulência/genética
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