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1.
Zhonghua Wei Chang Wai Ke Za Zhi ; 22(12): 1118-1123, 2019 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-31874526

RESUMO

As the rapid development of minimally invasive techniques, anesthesia, and enhanced recovery after surgery (ERAS), anorectal day surgery receiving more and more attention by improving efficiency of medical care while reducing cost and hospitalized infection. However, day surgery also faces the challenge of completing the whole process from patient admission to discharge within 24 hours. Therefore, establishing a reasonable and detailed day surgery process is the cornerstone to guarantee safe medical practice and patients satisfaction. National Clinical Research Center for Geriatric Disorders (Xiangya), together with China Ambulatory Surgery Alliance formulates the clinical practice guideline for anorectal day surgery 2019 edition. Here we make some interpretations of the guidelines on the detailed process of anorectal day surgery, including indication, preoperative examination, preoperative risk evaluation, health education, assessment of day surgery anesthesia and before leaving postanesthesia care unit (PACU), postoperative management, assessment of discharge and follow-up, for the convenience of various medical centers.


Assuntos
Procedimentos Cirúrgicos Ambulatórios/normas , Assistência ao Paciente/normas , Guias de Prática Clínica como Assunto/normas , Protectomia/normas , Idoso , Canal Anal/cirurgia , Humanos , Reto/cirurgia
3.
Ecotoxicology ; 23(10): 1958-68, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25149868

RESUMO

Environmental isotopology of sulfur and oxygen of dissolved sulfate in groundwater was conducted in the Hetao Plain, northwestern China, aiming to better understand the processes controlling arsenic mobilization in arsenic-rich aqueous systems. A total of 22 groundwater samples were collected from domestic wells in the Hetao Plain. Arsenic concentrations ranged from 11.0 to 388 µg/L. The δ(34)S-SO4 and δ(18)O-SO4 values of dissolved sulfate covered a range from +1.48 to +22.4‰ and +8.17‰ to +14.8‰ in groundwater, respectively. The wide range of δ(34)S-SO4 values reflected either an input of different sources of sulfate, such as gypsum dissolution and fertilizer application, or a modification from biogeochemical process of bacterial sulfate reduction. The positive correlation between δ(34)S-SO4 and arsenic concentrations suggested that bacteria mediated processes played an important role in the mobilization of arsenic. The δ(18)O-SO4 values correlated non-linearly with δ(34)S-SO4, but within a relatively narrow range (+8.17 to +14.8‰), implying that complexities inherent in the sulfate-oxygen (O-SO4(2-)) origins, for instance, water-derived oxygen (O-H2O), molecular oxygen (O-O2) and isotope exchanging with dissolved oxides, are accounted for oxygen isotope composition of dissolved sulfate in groundwater in the Hetao Plain.


Assuntos
Arsênico/análise , Monitoramento Ambiental/métodos , Água Subterrânea/química , Poluentes Químicos da Água/análise , China , Isótopos de Oxigênio/análise , Sulfatos/análise , Isótopos de Enxofre/análise
4.
Int J Tuberc Lung Dis ; 17(1): 113-9, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23232011

RESUMO

SETTING: Authorised clinical mycobacteriology laboratories in Taiwan. OBJECTIVE: To evaluate the impact of external quality assessment (EQA) on the quality of drug susceptibility testing (DST) in 2007-2011. DESIGN: Panels consisting of 20-30 Mycobacterium tuberculosis strains were used. Efficiency of 95% in detecting resistance to both isoniazid (INH) and rifampicin (RMP), and of 90% to ethambutol (EMB) and streptomycin (SM) was used to define a competent laboratory. RESULTS: The proportion of laboratories that fulfilled the competency criteria for all first-line drugs was 16.7% in 2007, increasing to 85.7% in 2008, 86.1% in 2009, 82.4% in 2010, and to 96.8% in 2011 (P < 0.01). The mean efficiency in detecting resistance to INH and RMP reached >99% during 2008-2011 (P = 0.90 for INH and P = 0.82 for RMP), and for EMB it increased from 82.0% in 2007 to 92.2% in 2008 and 99.5% in 2011 (P < 0.01), while that for resistance to SM increased from 82.0% in 2007 to 98.1% in 2008 and 99.5% in 2011 (P < 0.01). Preparations of inoculum for DST and detection of EMB resistance were the main reasons for non-competence. CONCLUSION: The EQA programme was effective in improving the competency of clinical laboratories in performing DST for tuberculosis.


Assuntos
Técnicas de Laboratório Clínico/normas , Mycobacterium tuberculosis/efeitos dos fármacos , Competência Profissional , Técnicas Bacteriológicas/normas , Humanos , Testes de Sensibilidade Microbiana , Taiwan , Fatores de Tempo
5.
J Infect Dis ; 206(2): 148-57, 2012 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-22561367

RESUMO

BACKGROUND: Oseltamivir resistance in A(H1N1)pdm09 influenza is rare, particularly in untreated community cases. Sustained community transmission has not previously been reported. METHODS: Influenza specimens from the Asia-Pacific region were collected through sentinel surveillance, hospital, and general practitioner networks. Clinical and epidemiological information was collected on patients infected with oseltamivir-resistant viruses. RESULTS: Twenty-nine (15%) of 191 A(H1N1)pdm09 viruses collected between May and September 2011 from Hunter New England (HNE), Australia, contained the H275Y neuraminidase substitution responsible for oseltamivir resistance. Only 1 patient had received oseltamivir before specimen collection. The resistant strains were genetically very closely related, suggesting the spread of a single variant. Ninety percent of cases lived within 50 kilometers. Three genetically similar oseltamivir-resistant variants were detected outside of HNE, including 1 strain from Perth, approximately 4000 kilometers away. Computational analysis predicted that neuraminidase substitutions V241I, N369K, and N386S in these viruses may offset the destabilizing effect of the H275Y substitution. CONCLUSIONS: This cluster represents the first widespread community transmission of H275Y oseltamivir-resistant A(H1N1)pdm09 influenza. These cases and data on potential permissive mutations suggest that currently circulating A(H1N1)pdm09 viruses retain viral fitness in the presence of the H275Y mutation and that widespread emergence of oseltamivir-resistant strains may now be more likely.


Assuntos
Antivirais/farmacologia , Surtos de Doenças , Farmacorresistência Viral , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/virologia , Oseltamivir/farmacologia , Adolescente , Adulto , Austrália/epidemiologia , Sequência de Bases , Criança , Pré-Escolar , Infecções Comunitárias Adquiridas , DNA Viral/química , Feminino , Humanos , Lactente , Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/epidemiologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Mutação , Neuraminidase/genética , Filogenia , Alinhamento de Sequência , Adulto Jovem
6.
Oral Dis ; 18(7): 707-12, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22490008

RESUMO

OBJECTIVE: This study aimed to evaluate the reliability and validity of the Chinese version of the Halitosis Associated Life-quality Test (HALT) questionnaire. METHODS: A total of 106 patients with oral malodour were recruited to complete the questionnaire after its translation and cross-cultural adaptation. The reliability of the Chinese version of the HALT was evaluated using internal consistency and test-retest methods. Both construct validity and discriminative validity were adopted to evaluate the validity of the HALT. RESULTS: The Cronbach's alpha value (internal reliability) for the total HALT score was 0.95, and the intraclass correlation coefficient (ICC) value (test-retest reliability) was 0.89 (95% CI = 0.74-0.98). The construct validity was determined by exploratory factor analysis. Four factors were extracted, which accounted for 85.18% of the variance. All items had factor loadings above 0.40, ranging from 0.53 to 0.94. In addition, the Chinese version of the HALT was found to be valid for distinguishing patients with different degrees of oral malodour. CONCLUSION: The results suggest that the Chinese version of the HALT has satisfactory psychometric properties and is applicable to patients with oral malodour in Chinese-speaking populations.


Assuntos
Halitose/psicologia , Qualidade de Vida , Inquéritos e Questionários , China , Humanos , Psicometria
7.
Euro Surveill ; 16(23)2011 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-21679678

RESUMO

A novel influenza A(H1N1)2009 variant with mildly reduced oseltamivir and zanamivir sensitivity has been detected in more than 10% of community specimens in Singapore and more than 30% of samples from northern Australia during the early months of 2011. The variant, which has also been detected in other regions of the Asia-Pacific, contains a S247N neuraminidase mutation. When combined with the H275Y mutation, as detected in an oseltamivir-treated patient, the dual S247N+H275Y mutant had extremely high oseltamivir resistance.


Assuntos
Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/epidemiologia , Influenza Humana/genética , Neuraminidase/genética , Oseltamivir/uso terapêutico , Polimorfismo de Nucleotídeo Único/genética , Zanamivir/uso terapêutico , Antivirais/uso terapêutico , Austrália/epidemiologia , Resistência a Medicamentos/genética , Predisposição Genética para Doença/epidemiologia , Predisposição Genética para Doença/genética , Variação Genética/genética , Humanos , Incidência , Influenza Humana/tratamento farmacológico , Neuraminidase/antagonistas & inibidores , Vigilância da População/métodos , Medição de Risco , Fatores de Risco , Singapura/epidemiologia
8.
Bull Environ Contam Toxicol ; 87(1): 91-5, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21562833

RESUMO

This study investigated metals of tailings from Tonglvshan mine in Daye and assessed the effect of metal contamination in water and sediment near the tailing reservoir. The concentration of copper, lead, zinc, cadmium, chromium and nickel was measured in deposit samples taken from a profile in an abandoned flotation tailing reservoir, as well as in water and sediment samples near the reservoir. The results of this study indicate that copper concentration ranges from 780 to 4390 mg/kg, 2-10 times higher than the limit values in soil, while the contents of other metals are below the limit values. Metal levels in water and sediments are high and varied widely in different sampling sites. The mean concentrations of copper, lead, zinc, cadmium, chromium and nickel in waters are 27.76, 2.28, 8.20, 0.12, 5.30 and 3.04 mg/L, while those in sediments are 557.65, 96.95, 285.20, 0.92, 94.30 and 4.75 mg/kg, respectively. All of the results indicate that the environment near the tailing reservoir is polluted to some extent by some kinds of metals, especially by copper, lead, zinc and cadmium, which may be caused not only by some discharge sources of metals, but also by life garbage and sewage.


Assuntos
Cobre/química , Sedimentos Geológicos/química , Ferro/química , Mineração , Sulfetos/química , Poluentes Químicos da Água/análise , Cádmio/análise , China , Cromo/análise , Cobre/análise , Monitoramento Ambiental/métodos , Ferro/análise , Chumbo/análise , Níquel/análise , Esgotos/análise , Sulfetos/análise , Zinco/análise
9.
Euro Surveill ; 16(3)2011 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-21262183

RESUMO

During the first year of the influenza A(H1N1) 2009 pandemic, unprecedented amounts of the neuraminidase inhibitors, predominantly oseltamivir, were used in economically developed countries for the treatment and prophylaxis of patients prior to the availability of a pandemic vaccine. Due to concerns about the development of resistance, over 1,400 influenza A(H1N1) 2009 viruses isolated from the Asia-Pacific region during the first year of the pandemic (March 2009 to March 2010) were analysed by phenotypic and genotypic assays to determine their susceptibility to the neuraminidase inhibitors. Amongst viruses submitted to the World Health Organization Collaborating Centre for Reference and Research in Melbourne, Australia,oseltamivir resistance was detected in 1.3% of influenza A(H1N1) 2009 strains from Australia and 3.1% of strains from Singapore, but none was detected in specimens received from other countries in Oceania or south-east Asia, or in east Asia. The overall frequency of oseltamivir resistance in the Asia-Pacific region was 16 of 1,488 (1.1%). No zanamivir-resistant viruses were detected. Of the 16 oseltamivir-resistant isolates detected, nine were from immunocompromised individuals undergoing oseltamivir treatment and three were from immunocompetent individuals undergoing oseltamivir treatment. Importantly, four oseltamivir-resistant strains were from immunocompetent individuals who had not been treated with oseltamivir, demonstrating limited low-level community transmission of oseltamivir-resistant strains. Even with increased use of oseltamivir during the pandemic, the frequency of resistance has been low, with little evidence of community-wide spread of the resistant strains. Nevertheless, prudent use of the neuraminidase inhibitors remains necessary, as does continued monitoring for drug-resistant influenza viruses.


Assuntos
Antivirais/farmacologia , Farmacorresistência Viral/genética , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Neuraminidase/genética , Oseltamivir/farmacologia , Ásia/epidemiologia , Austrália/epidemiologia , Humanos , Vírus da Influenza A Subtipo H1N1/genética , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Influenza Humana/epidemiologia , Influenza Humana/virologia , Testes de Sensibilidade Microbiana/métodos , Mutação , Neuraminidase/antagonistas & inibidores , Ilhas do Pacífico/epidemiologia , Pandemias , Filogenia , Vigilância da População , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência , Fatores de Tempo , Organização Mundial da Saúde
10.
Cell Death Dis ; 1: e94, 2010 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-21368870

RESUMO

Our previous studies have shown that microRNA-383 (miR-383) expression is downregulated in the testes of infertile men with maturation arrest (MA). However, the underlying mechanisms of miR-383 involved in the pathogenesis of MA remain unknown. In this study, we showed that downregulation of miR-383 was associated with hyperactive proliferation of germ cells in patients with mixed patterns of MA. Overexpression of miR-383 in NT2 (testicular embryonal carcinoma) cells resulted in suppression of proliferation, G1-phase arrest and induction of apoptosis, whereas silencing of miR-383 reversed these effects. The effects of miR-383 were mediated through targeting a tumor suppressor, interferon regulatory factor-1 (IRF1), and miR-383 was negatively correlated with IRF1 protein expression in vivo. miR-383 inhibited IRF1 by affecting its mRNA stability, which subsequently reduced the levels of the targets of IRF1, namely cyclin D1, CDK2 and p21. Downregulation of IRF1 or cyclin D1, but not that of CDK2, enhanced miR-383-mediated effects, whereas silencing of p21 partially inhibited the effects of miR-383. Moreover, miR-383 downregulated CDK4 by increasing proteasome-dependent degradation of CDK4, which in turn resulted in an inhibition of phosphorylated retinoblastoma protein (pRb) phosphorylation. These results suggest that miR-383 functions as a negative regulator of proliferation by targeting IRF1, in part, through inactivation of the pRb pathway. Abnormal testicular miR-383 expression may potentiate the connections between male infertility and testicular germ cell tumor.


Assuntos
Carcinoma Embrionário/genética , Infertilidade Masculina/genética , Fator Regulador 1 de Interferon/genética , MicroRNAs/metabolismo , Neoplasias Testiculares/genética , Animais , Apoptose , Linhagem Celular Tumoral , Proliferação de Células , Ciclina D1/metabolismo , Quinase 2 Dependente de Ciclina/metabolismo , Quinase 4 Dependente de Ciclina/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Regulação para Baixo , Células-Tronco de Carcinoma Embrionário/citologia , Fase G1 , Humanos , Infertilidade Masculina/metabolismo , Fator Regulador 1 de Interferon/metabolismo , Masculino , Camundongos , Fosforilação , Proteína do Retinoblastoma/metabolismo
11.
Antiviral Res ; 80(2): 200-5, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18611414

RESUMO

The adamantanes (amantadine and rimantadine) were the initial antivirals licensed for use against influenza A viruses and have been used in some countries to control seasonal influenza and have also been stockpiled for potential pandemic use. While high rates of resistance have been observed in recent years with A(H3) viruses, the rates of resistance with A(H1) viruses has varied widely. In this study we analysed 281 human influenza A viruses isolated in 2007 that were referred to the WHO Collaborating Centre for Reference and Research in Melbourne, mainly from Australia and the surrounding regions, for evidence of resistance to adamantanes and a subset of these was examined for resistance to the neuraminidase inhibitors (NIs). We found that the rates of adamantane resistance in A(H3) viruses continued to increase in most countries in 2007 but a distinct variation was seen with A(H1) resistance levels. A(H1) viruses from Australia, New Zealand and Europe had low rates of resistance (2-9%) whereas viruses from a number of South East (SE) Asian countries had high rates of resistance (33-100%). This difference can be attributed to the spread of A/Brisbane/59/2007-like viruses to many parts of the world with the exception of SE Asia where A/Hong Kong/2652/2006-like viruses continue to predominate. When these two A(H1) subgroups were compared for their in vitro sensitivity to the other class of influenza antiviral drugs, the neuraminidase inhibitors, no difference was seen between the groups with both showing normal levels of sensitivity to these drugs, The finding of reducing A(H1) resistance rates in Australia and rising levels in SE Asia in 2007, reverses the trend seen in 2006 when A(H1) resistance levels were rising in Australia and elsewhere but remained low in most of SE Asia.


Assuntos
Adamantano/uso terapêutico , Antivirais/uso terapêutico , Farmacorresistência Viral , Vírus da Influenza A/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Adamantano/farmacologia , Animais , Antivirais/farmacologia , Ásia Sudeste/epidemiologia , Austrália/epidemiologia , Linhagem Celular , Cães , Inibidores Enzimáticos/farmacologia , Inibidores Enzimáticos/uso terapêutico , Humanos , Vírus da Influenza A/classificação , Vírus da Influenza A/isolamento & purificação , Influenza Humana/epidemiologia , Influenza Humana/virologia , Neuraminidase/antagonistas & inibidores , Neuraminidase/metabolismo , Filogenia , Proteínas Virais/antagonistas & inibidores , Proteínas Virais/metabolismo
12.
J Appl Microbiol ; 98(1): 127-35, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15610425

RESUMO

AIMS: To develop food-grade cloning and expression vectors for use in genetic modification of Lactococcus lactis. METHODS AND RESULTS: Two plasmid replicons and three dominant selection markers were isolated from L. lactis and used to construct five food-grade cloning vectors. These vectors were composed of DNA only from L. lactis and contained no antibiotic resistance markers. Three of the vectors (pND632, pND648 and pND969) were based on the same plasmid replicon and carried, either alone or in combination, the three different selectable markers encoding resistance to nisin, cadmium and/or copper. The other two (pND965DJ and pND965RS) were derived from a cadmium resistance plasmid, and carried a constitutive promoter and a copper-inducible promoter, respectively, immediately upstream of a multicloning site. All vectors were stable in L. lactis LM0230 for at least 40 generations without selection pressure. The two groups of vectors were compatible in L. lactis LM0230. The vectors pND648 and pND965RS, as representatives of the two groups, were transferred successfully by electroporation into and maintained in an industrial strain of L. lactis. The usefulness of the vectors was further demonstrated by expressing a phage resistance gene (abiI) in another industrial strain of L. lactis. CONCLUSIONS: The five food-grade vectors constructed are potentially useful for industrial strains of L. lactis. SIGNIFICANCE AND IMPACT OF THE STUDY: These vectors represent a new set of molecular tools useful for food-grade modifications of L. lactis.


Assuntos
DNA/análise , Microbiologia de Alimentos , Lactococcus lactis/genética , Sequência de Bases , Clonagem Molecular , Expressão Gênica , Marcadores Genéticos , Vetores Genéticos , Dados de Sequência Molecular , Organismos Geneticamente Modificados , Regiões Promotoras Genéticas
13.
Acta Pharmacol Sin ; 22(12): 1121-4, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11749812

RESUMO

AIM: To observe polysaccharide of Spirulina platensis (PSp) on the hematopoietic system of mouse and dogs which were damaged by injection of cyclophosphamide (CTX) and 60Co-gamma irradiation. METHODS: CTX and 60Co gamma ray were used to induce bone marrow damage, and the experimental animals were ig with different dose of PSp in vivo, after 12-d and 21-d administration, the whole blood cells and nucleated cells in bone marrow were measured, and the DNA in bone marrow were inspected by UV-spectrophotometer. RESULTS: CTX and 60Co-gamma irradiation induced hemopoietic system damage in mice and dogs, respectively. PSp 30, 60 mg/kg increased the level of the white cells in blood and nucleated cells and DNA in bone marrow in mice but had no effects on red cells and hemoglobins. PSp 12 mg/kg increased the level of red cells, white cells, and hemoglobins in blood and nucleated cells in bone marrow in dogs (P < 0.01), and the effects of PSp 60 mg/kg were better than that of berbamine hydrochloride 60 mg/kg. CONCLUSION: PSp has chemo-protective and radio-protective capability, and may be a potential adjunct to cancer therapy.


Assuntos
Proteínas de Bactérias/química , Sistema Hematopoético/efeitos dos fármacos , Polissacarídeos/farmacologia , Protetores contra Radiação/farmacologia , Animais , Radioisótopos de Cobalto , Ciclofosfamida , Cães , Feminino , Hematínicos/farmacologia , Contagem de Leucócitos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Polissacarídeos/uso terapêutico , Lesões Experimentais por Radiação/sangue , Lesões Experimentais por Radiação/prevenção & controle , Distribuição Aleatória , Spirulina
14.
DNA Seq ; 11(3-4): 239-45, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-11092734

RESUMO

A plasmid-encoded type I restriction and modification (R-M) system, designated LldI, was identified in Lactococcus lactis biovar diacetylactis LD10-1. LldI consists of three genes encoding endonuclease, methylase and specificity subunits, respectively. RT-PCR analysis revealed that the three genes are co-transcribed as a polycistronic mRNA in L. lactis. The specificity subunit of LldI differs significantly in the target recognition domains from those of other type I R-M systems, suggesting that LldI confers a novel specificity in L. lactis.


Assuntos
Enzimas de Restrição do DNA/genética , Desoxirribonucleases de Sítio Específico do Tipo I/genética , Lactococcus lactis/enzimologia , Lactococcus lactis/genética , Plasmídeos , DNA Metiltransferases Sítio Específica (Adenina-Específica)/genética , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , Sequência Conservada , Enzimas de Restrição do DNA/química , Desoxirribonucleases de Sítio Específico do Tipo I/química , Genes Bacterianos , Dados de Sequência Molecular , Subunidades Proteicas , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , DNA Metiltransferases Sítio Específica (Adenina-Específica)/química , Transcrição Genética
15.
Nucleic Acids Res ; 28(23): E103, 2000 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-11095699

RESUMO

A simple and effective method based upon semi-specific PCR followed by cloning has been developed. Chromosomal mapping of the generated fragment on a somatic cell hybrid panel identifies the chromosomal position, and yields a unique sequence tag for the site. Using this method, the chromosomal location of one porcine endogenous retrovirus (PERV) was determined. The porcine genomic sequences were first amplified by PCR using a PERV-specific primer and a porcine short interspersed nuclear element (SINE)-specific primer. PCR products were cloned, and those sequences that contained PERV plus flanking regions were selected using a second round of PCR and cloning. Sequences flanking the PERV were determined and a PERV-B was physically mapped on porcine chromosome 17 using a somatic hybrid panel. The general utility of the method was subsequently demonstrated by locating PERVs in the genome of PERV infected human 293 cells. This method obviates the need for individual library construction or linker/adaptor ligation, and can be used to quickly locate individual sites of moderately repeated, dispersed DNA sequences in any genome.


Assuntos
Mapeamento Cromossômico , Sequências Repetitivas de Ácido Nucleico/genética , Animais , Linhagem Celular , Clonagem Molecular , DNA/química , DNA/genética , Retrovirus Endógenos/genética , Feminino , Humanos , Masculino , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Mapeamento de Híbridos Radioativos , Análise de Sequência de DNA , Suínos
16.
Transplantation ; 70(7): 1010-6, 2000 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-11045635

RESUMO

BACKGROUND: Xenotransplantation using pig organs or tissues may alleviate the human donor organ shortage. However, one concern is the potential transmission of pig pathogens to humans, especially pig endogenous retroviruses (PERV), which infect human cell lines in vitro. In this report, the cross-species in vivo transmission of PERV by xenotransplantation was studied using a severe combined immunodeficient (SCID) mouse model. METHODS: Twenty-one SCID mice were transplanted with fetal pig pancreatic cells and left for periods from three to 41 weeks before being killed. DNA and RNA were extracted from liver, spleen, and brain of these mice, and examined for PERV using nested polymerase chain reaction (PCR) and reverse transcriptase-PCR. The pig mitochondrial cytochrome oxidase II subunit gene (COII) was also amplified to monitor the presence of pig cell microchimerism in xenotransplanted tissues, and a housekeeping gene was included to monitor the DNA quality and quantity. RESULTS: Examination of 39 DNA samples from tissues of the 21 xenografted mice identified two mouse tissues (M4-liver and M19-spleen) that were positive for PERV but negative for COII. A total of 23 (59%) of the mouse tissues were positive for both PERV and COII, 6 (16%) were negative for both, and 8 (20%) were positive for COII only. PCR and direct sequencing of the PCR products identified three PERV variants, which were different from the PERV sequence detected by PCR direct sequencing from the pig donor cells. CONCLUSIONS: The PERV+/COII- results from M4-liver and M19-spleen indicated the presence of PERV transmission from pig to mouse tissue. The PERV variants detected in the mouse tissues indicated that different PERVs were transmissible from the pig to mouse tissue during xenotransplantation. The negative reverse transcriptase-PCR results for PERV from three mouse samples including M4-liver and M19-spleen suggest there was no active PERV transcription in the mouse tissues, although this would need to be studied further.


Assuntos
Transplante das Ilhotas Pancreáticas/imunologia , Infecções por Retroviridae/transmissão , Transplante Heterólogo/imunologia , Animais , Sequência de Bases , DNA Viral/química , Feto , Ilhotas Pancreáticas/embriologia , Camundongos , Camundongos SCID , Modelos Animais , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Retroviridae/genética , Suínos , Replicação Viral/fisiologia
17.
J Biotechnol ; 67(2-3): 135-49, 1999 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-9990732

RESUMO

A plasmid-encoded phage abortive infection mechanism (AbiL) was identified from Lactococcus lactis biovar. diacetylactis LD10-1. AbiL conferred complete resistance to the small isometric-headed phage phi 712 (936 species) and partial resistance to the prolate-headed phage phi c2 (c2 species) when introduced into L. lactis LM0230. However, AbiL was not effective against the small isometric-headed phage ul36 (P335 species). The AbiL determinant was sequenced and it consists of two open reading frames, abiLi and abiLii. Their encoded proteins did not share significant homology with any known proteins in the protein databases. Transcriptional analysis indicated that abiLi and abiLii are organized as a single operon. Deletion within abiLii abolished the phage resistance. The levels of four phi c2-specific transcripts, three within the early transcribed region and one within the late transcribed region, were examined by RT-PCR, no effect of AbiL on synthesis of these transcripts was detected, suggesting that AbiL may act at a point after the transcription of phi c2 in L. lactis.


Assuntos
Bacteriófagos/fisiologia , Genes Bacterianos , Lactococcus lactis/genética , Sequência de Aminoácidos , Bacteriófagos/genética , Sequência de Bases , Deleção de Genes , Lactococcus lactis/virologia , Dados de Sequência Molecular , Plasmídeos/genética , RNA Bacteriano/química , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Transcrição Genética
19.
Quintessence Int ; 29(1): 13-9, 1998 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9611470

RESUMO

Elongated and spaced incisors are common in patients suffering from severe periodontal disease. Intrusion and uprighting of incisors might be the logical solution for this problem. This article describes a team approach to treatment planning for adult patients with severe localized periodontitis accompanied by marginal bone loss and spacing and elongation of incisors. The treatment involves the combination of periodontal treatment, orthodontic intrusion, and prosthetic therapy. Controlled intrusion in two patients led to a decrease in the clinical crown length, better access for oral hygiene procedures, better gingival form, and a more suitable distribution of occlusal forces.


Assuntos
Perda do Osso Alveolar/complicações , Incisivo/fisiopatologia , Má Oclusão/terapia , Periodontite/complicações , Técnicas de Movimentação Dentária , Adulto , Perda do Osso Alveolar/etiologia , Feminino , Humanos , Masculino , Má Oclusão/etiologia , Equipe de Assistência ao Paciente , Periodontite/terapia
20.
FEMS Microbiol Lett ; 146(1): 149-54, 1997 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-8997719

RESUMO

A 16-kb plasmid (pND859) was identified from Lactococcus lactis biovar. diacetylactis UK12922 which encodes phage resistance to the small isometric phage 712 when tested in L. lactis LM0230. The gene encoding phage abortive infection, designated abi-859, was localized on a 1.2-kb region which consists of an open reading frame (ORF) of 846 bp preceded by a potential ribosome-binding site and a putative promoter region. A helix-turn-helix region typical of DNA-binding motifs was identified near the N-terminal of the abi-859 product, suggesting a possible interaction with the phage DNA.


Assuntos
Bacteriófagos/genética , Lactococcus lactis/genética , Lactococcus lactis/virologia , Plasmídeos/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Dados de Sequência Molecular , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos
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