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2.
J Agric Food Chem ; 69(38): 11154-11162, 2021 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-34529438

RESUMO

In this study, the enantioselective activity of the chiral fungicide triticonazole (TRZ) against target pathogens of seed-borne diseases and the effect on the early phenotypic indexes and physiological and biochemical indexes of wheat following the soaking of seeds were studied. S-TRZ had low fungicidal activity and significantly inhibited the germination of wheat seeds. Seedlings in the S-TRZ treatment group suffered severe oxidative damage; the contents of glutathione (GSH) and malondialdehyde (MDA) were significantly increased. S-TRZ significantly inhibited the synthesis of gibberellin (GA) and ethylene (ETH), while it significantly increased that of jasmonic acid (JA). R-TRZ showed the highest activity against two smut fungi; the treatment of seeds with 5 g (ai)/100 kg R-TRZ had little influence on early wheat growth. The plant hormones were synthesized normally; seedlings grew well, and the fresh weight increased significantly following this treatment. Based on these data, we propose to develop and apply R-TRZ using the national recommended doses to control seed-borne diseases of wheat.


Assuntos
Praguicidas , Triticum , Ciclopentanos , Germinação , Plântula , Sementes , Estereoisomerismo , Triazóis/toxicidade
3.
J Ovarian Res ; 14(1): 120, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34526089

RESUMO

BACKGROUND: Ovarian cancer(OC) is the gynecological tumor with the highest mortality rate, effective biomarkers are of great significance in improving its prognosis. In recent years, there have been many studies on alternative splicing (AS) events, and the role of AS events in tumor has become a focus of attention. METHODS: Data were downloaded from the TCGA database and Univariate Cox regression analysis was performed to determine AS events associated with OC prognosis.Eight prognostic models of OC were constructed in R package, and the accuracy of the models were evaluated by the time-dependent receiver operating characteristic (ROC) curves.Eight types of survival curves were drawn to evaluate the differences between the high and low risk groups.Independent prognostic factors of OC were analyzed by single factor independent analysis and multi-factor independent prognostic analysis.Again, Univariate Cox regression analysis was used to analyze the relationship between splicing factors(SF) and AS events, and Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were performed on OS-related SFs to understand the pathways. RESULTS: Univariate Cox regression analysis showed that among the 15,278 genes, there were 31,286 overall survival (OS) related AS events, among which 1524 AS events were significantly correlated with OS. The area under the time-dependent receiver operating characteristic curve (AUC) of AT and ME were the largest and the RI was the smallest,which were 0.757 and 0.68 respectively. The constructed models have good value for the prognosis assessment of OC patients. Among the eight survival curves, AP was the most significant difference between the high and low risk groups, with a P value of 1.61e - 1.The results of single factor independent analysis and multi-factor independent prognostic analysis showed that risk score calculated by the model and age could be used as independent risk factors.According to univariate COX regression analysis,109 SFs were correlated with AS events and adjusted in two ways: positive and negative. CONCLUSIONS: SFs and AS events can directly or indirectly affect the prognosis of OC patients. It is very important to find effective prognostic markers to improve the survival rate of OC.

4.
J Hazard Mater ; 416: 125911, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492845

RESUMO

Plant growth can be influenced by the application of triazole pesticides as these regulate physiological processes such as plant hormonal levels and enzyme activity. Homology modeling and molecular docking studies suggested that inhibition of ADP-glucose pyrophosphorylase activity in two trans-stereoisomers treatments hinders starch accumulation during the grain filling stage. A field experiment investigated the effects of metconazole racemate, cis-1R,5S-stereostereoisomer, and cis-1S,5R-stereoisomer application at the flowering stage on wheat grain ripening and yield. The concentrations of racemate and both cis-stereoisomers were detected in wheat plant and grain samples. Compared with the racemate, both cis-stereoisomers were more persistent in the matrices. Treatment with cis-1R,5S-stereoisomer decreased grain weight and yield of wheat by delaying chlorophyll degradation, increasing the ethylene content, and decreasing the level of abscisic acid. The germination of harvested seeds was adversely affected by racemate treatment as a result of gibberellin and abscisic acid metabolism regulation and the transcription of signaling-related genes. Therefore, cis-1S,5R-stereoisomer was recommended to be used as metconazole pesticide at the flowering stage.


Assuntos
Praguicidas , Triticum , Germinação , Simulação de Acoplamento Molecular , Sementes , Triazóis
5.
Signal Transduct Target Ther ; 6(1): 329, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34471087

RESUMO

It's a challenge for detecting the therapeutic targets of a polypharmacological drug from variations in the responsed networks in the differentiated populations with complex diseases, as stable coronary heart disease. Here, in an adaptive, 31-center, randomized, double-blind trial involving 920 patients with moderate symptomatic stable angina treated by 14-day Danhong injection(DHI), a kind of polypharmacological drug with high quality control, or placebo (0.9% saline), with 76-day following-up, we firstly confirmed that DHI could increase the proportion of patients with clinically significant changes on angina-frequency assessed by Seattle Angina Questionnaire (ΔSAQ-AF ≥ 20) (12.78% at Day 30, 95% confidence interval [CI] 5.86-19.71%, P = 0.0003, 13.82% at Day 60, 95% CI 6.82-20.82%, P = 0.0001 and 8.95% at Day 90, 95% CI 2.06-15.85%, P = 0.01). We also found that there were no significant differences in new-onset major vascular events (P = 0.8502) and serious adverse events (P = 0.9105) between DHI and placebo. After performing the RNA sequencing in 62 selected patients, we developed a systemic modular approach to identify differentially expressed modules (DEMs) of DHI with the Zsummary value less than 0 compared with the control group, calculated by weighted gene co-expression network analysis (WGCNA), and sketched out the basic framework on a modular map with 25 functional modules targeted by DHI. Furthermore, the effective therapeutic module (ETM), defined as the highest correlation value with the phenotype alteration (ΔSAQ-AF, the change in SAQ-AF at Day 30 from baseline) calculated by WGCNA, was identified in the population with the best effect (ΔSAQ-AF ≥ 40), which is related to anticoagulation and regulation of cholesterol metabolism. We assessed the modular flexibility of this ETM using the global topological D value based on Euclidean distance, which is correlated with phenotype alteration (r2: 0.8204, P = 0.019) by linear regression. Our study identified the anti-angina therapeutic module in the effective population treated by the multi-target drug. Modular methods facilitate the discovery of network pharmacological mechanisms and the advancement of precision medicine. (ClinicalTrials.gov identifier: NCT01681316).

6.
Cell Transplant ; 30: 9636897211045319, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34586895

RESUMO

Apart from aging process, adult intervertebral disc (IVD) undergoes various degenerative processes. However, the nicotine has not been well identified as a contributing etiology. According to a few studies, nicotine ingestion through smoking, air or clothing may significantly accumulate in active as well as passive smokers. Since nicotine has been demonstrated to adversely impact various physiological processes, such as sympathetic nervous system, leading to impaired vasculature and cellular apoptosis, we aimed to investigate whether nicotine could induce IVD degeneration. In particular, we evaluated dose-dependent impact of nicotine in vitro to simulate its chronic accumulation, which was later treated by platelet-derived biomaterials (PDB). Further, during in vivo studies, mice were subcutaneously administered with nicotine to examine IVD-associated pathologic changes. The results revealed that nicotine could significantly reduce chondrocytes and chondrogenic indicators (Sox, Col II and aggrecan). Mice with nicotine treatment also exhibited malformed IVD structure with decreased Col II as well as proteoglycans, which was significantly increased after PDB administration for 4 weeks. Mechanistically, PDB significantly restored the levels of IGF-1 signaling proteins, particularly pIGF-1 R, pAKT, and IRS-1, modulating ECM synthesis by chondrocytes. Conclusively, the PDB impart reparative and tissue regenerative processes by inhibiting nicotine-initiated IVD degeneration, through regulating IGF-1/AKT/IRS-1 signaling axis.

7.
N Engl J Med ; 385(14): 1268-1279, 2021 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-34491661

RESUMO

BACKGROUND: The appropriate target for systolic blood pressure to reduce cardiovascular risk in older patients with hypertension remains unclear. METHODS: In this multicenter, randomized, controlled trial, we assigned Chinese patients 60 to 80 years of age with hypertension to a systolic blood-pressure target of 110 to less than 130 mm Hg (intensive treatment) or a target of 130 to less than 150 mm Hg (standard treatment). The primary outcome was a composite of stroke, acute coronary syndrome (acute myocardial infarction and hospitalization for unstable angina), acute decompensated heart failure, coronary revascularization, atrial fibrillation, or death from cardiovascular causes. RESULTS: Of the 9624 patients screened for eligibility, 8511 were enrolled in the trial; 4243 were randomly assigned to the intensive-treatment group and 4268 to the standard-treatment group. At 1 year of follow-up, the mean systolic blood pressure was 127.5 mm Hg in the intensive-treatment group and 135.3 mm Hg in the standard-treatment group. During a median follow-up period of 3.34 years, primary-outcome events occurred in 147 patients (3.5%) in the intensive-treatment group, as compared with 196 patients (4.6%) in the standard-treatment group (hazard ratio, 0.74; 95% confidence interval [CI], 0.60 to 0.92; P = 0.007). The results for most of the individual components of the primary outcome also favored intensive treatment: the hazard ratio for stroke was 0.67 (95% CI, 0.47 to 0.97), acute coronary syndrome 0.67 (95% CI, 0.47 to 0.94), acute decompensated heart failure 0.27 (95% CI, 0.08 to 0.98), coronary revascularization 0.69 (95% CI, 0.40 to 1.18), atrial fibrillation 0.96 (95% CI, 0.55 to 1.68), and death from cardiovascular causes 0.72 (95% CI, 0.39 to 1.32). The results for safety and renal outcomes did not differ significantly between the two groups, except for the incidence of hypotension, which was higher in the intensive-treatment group. CONCLUSIONS: In older patients with hypertension, intensive treatment with a systolic blood-pressure target of 110 to less than 130 mm Hg resulted in a lower incidence of cardiovascular events than standard treatment with a target of 130 to less than 150 mm Hg. (Funded by the Chinese Academy of Medical Sciences and others; STEP ClinicalTrials.gov number, NCT03015311.).

8.
Mol Psychiatry ; 2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34381170

RESUMO

Genome-wide association studies (GWASs) have discovered numerous risk genes for Alzheimer's disease (AD), but how these genes confer AD risk is challenging to decipher. To efficiently transform genetic associations into drug targets for AD, we employed an integrative analytical pipeline using proteomes in the brain and blood by systematically applying proteome-wide association study (PWAS), Mendelian randomization (MR) and Bayesian colocalization. Collectively, we identified the brain protein abundance of 7 genes (ACE, ICA1L, TOM1L2, SNX32, EPHX2, CTSH, and RTFDC1) are causal in AD (P < 0.05/proteins identified for PWAS and MR; PPH4 >80% for Bayesian colocalization). The proteins encoded by these genes were mainly expressed on the surface of glutamatergic neurons and astrocytes. Of them, ACE with its protein abundance was also identified in significant association with AD on the blood-based studies and showed significance at the transcriptomic level. SNX32 was also found to be associated with AD at the blood transcriptomic level. Collectively, our current study results on genetic, proteomic, and transcriptomic approaches has identified compelling genes, which may provide important leads to design future functional studies and potential drug targets for AD.

9.
Asian Nurs Res (Korean Soc Nurs Sci) ; 15(3): 215-221, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34216818

RESUMO

PURPOSE: The aim of this study was to examine the behavioral responses of pregnant women during the early stage of Coronavirus Disease 2019 (COVID-19) outbreak. METHODS: We recruited 1,099 women to complete an online questionnaire survey from February 10 to February 25, 2020. The subjects were divided into two groups (the pregnant women group and the control group). RESULTS: Concerns about infection: most of the participants watched the COVID-19 news at least once a day. Protective behaviors: the utilization rate of pregnant women (often using various measures) was higher than that of nonpregnant women. Exercise: 30.6% of the pregnant women continued to exercise at home, whereas in the control group, this percentage was 8.4%. Spouse relationship: 38.8% of the subjects' relationship improved, whereas only 2.3% thought the relationship was getting worse. CONCLUSION: Pregnant women had some unique behavioral responses different from that of nonpregnant women. It is important to understand the behavioral responses of pregnant women in this network era.


Assuntos
Ansiedade/epidemiologia , Ansiedade/psicologia , COVID-19/psicologia , Depressão/psicologia , Complicações Infecciosas na Gravidez/psicologia , Gestantes/psicologia , Adulto , COVID-19/epidemiologia , China , Estudos Transversais , Depressão/epidemiologia , Feminino , Humanos , Gravidez , Complicações Infecciosas na Gravidez/prevenção & controle
10.
11.
Chaos ; 31(4): 043103, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34251268

RESUMO

In this paper, a simple chaotic memristor-based circuit with an external stimulation is proposed, and its basic dynamic properties are demonstrated. When the external perturbation becomes time varying and its frequency is low enough, the system has two-time scales, which can be employed to explore the mechanisms of symmetrical Hopf-induced bursting oscillations and delay effects. Furthermore, delay-times on Hopf-induced bursting at different frequencies of the external stimulation are measured. The results show that the relationship between the delay-time and external frequency is subject to a power law. In order to enhance the existing chaos of the system, a 4D system is developed by adding a nonlinear state feedback controller, which shows hyperchaos under some suitable parameters. These two systems are implemented on Multisim and hardware platforms, and the corresponding experimental results verify the correctness of the numerical simulations.

12.
Small ; 17(33): e2101798, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34228391

RESUMO

Reversible electrodeposition of metals at liquid-solid interfaces is a requirement for long cycle life in rechargeable batteries that utilize metals as anodes. The process has been studied extensively from the perspective of the electrochemical transformations that impact reversibility, however, the fundamental challenges associated with maintaining morphological control when a intrinsically crystalline solid metal phase emerges from an electrolyte solution have been less studied, but provide important opportunities for progress. A crystal growth stabilization method to reshape the initial growth and orientation of crystalline metal electrodeposits is proposed here. The method takes advantage of polymer-salt complexes (PEG-Zn2+ -aX- ) (a = 1,2,3) formed spontaneously in aqueous electrolytes containing zinc (Zn2+ ) and halide (X- ) ions to regulate electro-crystallization of Zn. It is shown that when X = Iodine (I), the complexes facilitate electrodeposition of Zn in a hexagonal closest packed morphology with preferential orientation of the (002) plane parallel to the electrode surface. This facilitates exceptional morphological control of Zn electrodeposition at planar substrates and leads to high anode reversibility and unprecedented cycle life. Preliminary studies of the practical benefits of the approach are demonstrated in Zn-I2 full battery cells, designed in both coin cell and single-flow battery cell configurations.

13.
Blood Purif ; : 1-11, 2021 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-34175850

RESUMO

BACKGROUND: Erythropoiesis-stimulating agents (ESAs) constitute an important treatment option for anemia in hemodialysis (HD) patients. We investigated the relationships among the dosage of ESA, erythropoietin resistance index (ERI) scores, and mortality in Chinese MHD patients. METHODS: This multicenter observational retrospective study included MHD patients from 16 blood purification centers (n = 824) who underwent HD in 2011-2015 and were followed up until December 31, 2016. We collected demographic variables, HD parameters, laboratory values, and ESA dosages. Patients were grouped into quartiles according to ESA dosage to study the effect of ESA dosage on all-cause mortality. The ERI was calculated as follows: ESA (IU/week)/weight (kg)/hemoglobin levels (g/dL). We also compared outcomes among the patients stratified into quartiles according to ERI scores. We used the Cox proportional hazards model to measure the relationships between the ESA dosage, ERI scores, and all-cause mortality. Using propensity score matching, we compared mortality between groups according to ERI scores, classified as either > or ≤12.80. RESULTS: In total, 824 patients were enrolled in the study; 200 (24.3%) all-cause deaths occurred within the observation period. Kaplan-Meier analyses showed that patients administered high dosages of ESAs had significantly worse survival than those administered low dosages of ESAs. A multivariate Cox regression identified that high dosages of ESAs could significantly predict mortality (ESA dosage >10,000.0 IU/week, HR = 1.59, 95% confidence intervals (CIs) (1.04, 2.42), and p = 0.031). Our analysis also indicated a significant increase in the risk of mortality in patients with high ERI scores. Propensity score matching-analyses confirmed that ERI > 12.80 could significantly predict mortality (HR = 1.56, 95% CI [1.11, 2.18], and p = 0.010). CONCLUSIONS: Our data suggested that ESA dosages >10,000.0 IU/week in the first 3 months constitute an independent predictor of all-cause mortality among Chinese MHD patients. A higher degree of resistance to ESA was related to a higher risk of all-cause mortality.

14.
IEEE J Biomed Health Inform ; 25(10): 3995-4004, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34018940

RESUMO

Medication combination prediction can be applied to the clinical treatment for critical patients with multi-morbidity. The suitable medication combination can help cure patients and keep the treatment medication safe. However, the complexity and uncertainty of clinical circumstances limit the predictive accuracy of medication combination. Thus, this paper proposes a new medication combination prediction model based on the temporal attention mechanism (TAM) and the simple graph convolution (SGC), named as TAMSGC. More specifically, the TAM can capture the temporal sequence information in the medical records, and the SGC is implemented to acquire the medication knowledge from the complicated medication combination. Experiments in a real dataset show that TAMSGC surpasses the baseline models on the predictive accuracy of medication combination.

15.
Plant Dis ; 2021 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-33999711

RESUMO

Brassica juncea var. multisecta, a leafy mustard, is widely grown in China as a vegetable (Fahey 2016). In May 2018, blackleg symptoms, grayish lesions with black pycnidia, were found on stems and leaves of B. juncea var. multisecta during disease surveys in Wuhan, Hubei Province. Disease incidence was approximately 82% of plants in the surveyed fields (~1 ha in total). To determine the causal agent of the disease, twelve diseased petioles were surface-sterilized and then cultured on potato dextrose agar (PDA) at 20˚C for 5 days. Six fungal isolates (50%) were obtained. All showed fluffy white aerial mycelia on the colony surface and produced a yellow pigment in PDA. In addition, pink conidial ooze formed on top of pycnidia after 20 days of cultivation on a V8 juice agar. Pycnidia were black-brown and globose with average size of 145 × 138 µm and ranged between 78 to 240 × 71 to 220 µm, n = 50. The conidia were cylindrical, hyaline, and 5.0 × 2.1 µm (4 to 7.1 × 1.4 to 2.9 µm, n=100). These results indicated that the fungus was Leptosphaeria biglobosa rather than L. maculans, as only the former produces yellow pigment (Williams and Fitt 1999). For molecular confirmation of identify, genomic DNAs were extracted and tested through polymerase chain reaction (PCR) assay using the species-specific primers LbigF, LmacF, and LmacR (Liu et al. 2006), of which DNA samples of L. maculans isolate UK-1 (kindly provided by Dr. Yongju Huang of University of Hertfordshire) and L. biglobosa 'brassicae' isolate B2003 (Cai et al. 2014) served as controls. Moreover, the sequences coding for actin, ß-tubulin, and the internal transcribed spacer (ITS) region of ribosomal DNA (Vincenot et al. 2008) of isolates HYJ-1, HYJ-2 and HYJ-3 were also cloned and sequenced. All six isolates only produced a 444-bp DNA fragment, the same as isolate B2003, indicating they belonged to L. biglobosa 'brassicae', as L. maculans generates a 331-bp DNA fragment. In addition, sequences of ITS (GenBank accession no. MN814012, MN814013, MN814014), actin (MN814292, MN814293, MN814294), and ß-tubulin (MN814295, MN814296, MN814297) of isolates HYJ-1, HYJ-2 and HYJ-3 were 100% identical to the ITS (KC880981), actin (AY748949), and ß-tubulin (AY748995) of L. biglobosa 'brassicae' strains in GenBank, respectively. To determine their pathogenicity, needle-wounded cotyledons (14 days) of B. juncea var. multisecta 'K618' were inoculated with a conidial suspension (1 × 107 conidia/ml, 10 µl per site) of two isolates HYJ-1 and HYJ-3, twelve seedlings per isolate (24 cotyledons), while the control group was only treated with sterile water. All seedlings were incubated in a growth chamber (20°C, 100% relative humidity under 12 h of light/12 h of dark) for 10 days. Seedlings inoculated with conidia showed necrotic lesions, whereas control group remained asymptomatic. Two fungal isolates showing the same culture morphology to the original isolates were re-isolated from the necrotic lesions. Therefore, L. biglobosa 'brassicae' was confirmed to be the causal agent of blackleg on B. juncea var. multisecta in China. L. biglobosa 'brassicae' has been reported on many Brassica crops in China, such as B. napus (Fitt et al. 2006), B. oleracea (Zhou et al. 2019), B. juncea var. multiceps (Zhou et al. 2019), B. juncea var. tumida (Deng et al. 2020). To our knowledge this is the first report of L. biglobosa 'brassicae' causing blackleg on B. juncea var. multisecta in China, and its occurrence might be a new threat to leafy mustard production of China.

16.
Biomed Pharmacother ; 140: 111689, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34004510

RESUMO

Vascular smooth muscle cell (VSMC) phenotypic switch plays an essential role in the pathogenesis of hypertension. Mitochondrial dynamics, such as mitochondrial fission, can also contribute to VSMC phenotypic switch. Whether mitochondrial fission act as a novel target for anti-hypertensive drug development remains unknown. In the present study, we confirmed that angiotensin II (AngII) rapidly and continuously induced mitochondrial fission in VSMCs. We also detected the phosphorylation status of dynamin-related protein-1 (Drp1), a key protein involved in mitochondrial fission, at Ser616 site; and observed Drp1 mitochondrial translocation in VSMCs or arteries of AngII-induced hypertensive mice. The Drp1 inhibitor mitochondrial division inhibitor-1 (Mdivi-1) dramatically reversed AngII-induced Drp1 phosphorylation, mitochondrial fission, and reactive oxidative species generation. Treatment with Mdivi-1 (20 mg/kg/every other day) significantly attenuated AngII-induced hypertension (22 mmHg), arterial remodeling, and cardiac hypertrophy, in part by preventing VSMC phenotypic switch. In addition, Mdivi-1 treatment was not associated with liver or renal functional injury. Collectively, these results indicate that Mdivi-1 inhibited mitochondrial fission, recovered mitochondrial activity, and prevented AngII-induced VSMC phenotypic switch, resulting in reduced hypertension.


Assuntos
Angiotensina II/farmacologia , Hipertensão/tratamento farmacológico , Mitocôndrias/efeitos dos fármacos , Dinâmica Mitocondrial/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Quinazolinonas/farmacologia , Animais , Anti-Hipertensivos/farmacologia , Células Cultivadas , Dinaminas/metabolismo , Hipertensão/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/metabolismo , Proteínas Mitocondriais/metabolismo , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/metabolismo , Fosforilação/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo
17.
Life Sci ; 277: 119501, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33862108

RESUMO

AIMS: The present study evaluated the functions of Piperlongumine (PL) in osteosarcoma (OS) cell growth and metastasis both in vitro and in vivo. MAIN METHODS: MTT assay was conducted to test the cytotoxic effects of PL on the human osteoblasts line HFOB1.19 and the human normal chondrocyte line C28/I2T. FITC-Annexin V and propidium iodide (PI) were used to examine cell apoptosis. The migration, invasion and relative epithelial-mesenchymal transition were examined by Transwell assay and Western blotting. Reverse transcription-quantitative PCR (RT-qPCR) was performed to analyze the cytokine signaling 3 (SOCS3) mRNA expression. TargetScan database was used to predict the target of SOCS3. The binding association between miR-30d-5p and SOCS3 in U2OS and MG63 cells was evaluated by the dual-luciferase reporter assay. A xenograft model was constructed to evaluate the effect of PL on OS cell growth in vivo. KEY FINDINGS: The results revealed that PL inhibited the growth, migration, invasion, epithelial-mesenchymal transition, and promoted the apoptosis of OS cells dose-dependently. In addition, PL upregulated the protein levels of suppressor of SOCS3, while it inactivated the JAK2/STAT3 pathway, which was accompanied by a decreased level of microRNA (miR)-30d-5p. Furthermore, SOCS3was confirmed as a novel target of miR-30d-5p. Overexpression of miR-30d-5p not only led to decreased expression of SOCS3, but also dampened the antitumor effect of PL on OS. SIGNIFICANCE: The present data demonstrated that PL inhibited the progression of OS via downregulation of the SOCS3-mediated JAK2/STAT3 pathway by inhibiting miR-30d-5p.


Assuntos
Dioxolanos/farmacologia , MicroRNAs/genética , Osteossarcoma/metabolismo , Animais , Apoptose/genética , Movimento Celular/genética , Proliferação de Células/genética , Dioxolanos/metabolismo , Progressão da Doença , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Janus Quinase 2/metabolismo , Camundongos , Camundongos Nus , MicroRNAs/metabolismo , Osteossarcoma/tratamento farmacológico , Osteossarcoma/genética , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais/genética , Proteína 3 Supressora da Sinalização de Citocinas/metabolismo
18.
Biomed Pharmacother ; 139: 111593, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33865018

RESUMO

BACKGROUND: Cerebral ischemic events, comprising of excitotoxicity, reactive oxygen production, and inflammation, adversely impact the metabolic-redox circuit in highly active neuronal metabolic profile which maintains energy-dependent brain activities. Therefore, we investigated neuro-regenerative potential of melatonin (Mel), a natural biomaterial secreted by pineal gland. METHODS: We specifically determined whether Mel could influence tunneling nanotubes (TNTs)-mediated transfer of functional mitochondria (Mito) which in turn may alter membrane potential, oxidative stress and apoptotic factors. In vitro studies assessed the effects of Mito on levels of cytochrome C, mitochondrial transfer, reactive oxygen species, membrane potential and mass, which were all further enhanced by Mel pre-treatment, whereas in vivo studies examined brain infarct area (BIA), neurological function, inflammation, brain edema and integrity of neurons and myelin sheath in control, ischemia stroke (IS), IS + Mito and IS + Mel-Mito group rats. RESULTS: Results showed that Mel pre-treatment significantly increased mitochondrial transfer and antioxidants, and inhibited apoptosis. Mel-pretreated Mito also significantly reduced BIA with improved neurological function. Apoptotic, oxidative-stress, autophagic, mitochondrial/DNA-damaged biomarkers indices were also improved. CONCLUSION: Conclusively, Mel is a potent biomaterial which could potentially impart neurogenesis through repairing impaired metabolic-redox circuit via enhanced TNT-mediated mitochondrial transfer, anti-oxidation, and anti-apoptotic activities in ischemia.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Encéfalo/efeitos dos fármacos , Melatonina/farmacologia , Fármacos Neuroprotetores/farmacologia , Animais , Apoptose/efeitos dos fármacos , Encéfalo/metabolismo , Linhagem Celular Tumoral , Peróxido de Hidrogênio/farmacologia , Masculino , Camundongos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Nanotubos , Neurogênese/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Consumo de Oxigênio/efeitos dos fármacos , Ratos Sprague-Dawley , Regulação para Cima
19.
Environ Sci Pollut Res Int ; 28(29): 38909-38928, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33745048

RESUMO

The current study aims to analyze the regional differences and spatiotemporal dynamic evolution of carbon emission intensity (CEI) and carbon emission per capita (CEPC) of planting industry with consideration of carbon sink effect. The results indicate that: (i) The CEI and CEPC of China's planting industry present significant non-equilibrium distribution characteristic during the investigate period, provinces with high CEI are mainly distributed in major agricultural provinces, while high CEPC provinces are mainly located in northeast and individual central provinces with large planting industry. (ii) Inter-regional difference is the principal course of the total differences, the CEI Theil index demonstrates gradient decreasing pattern of "western > central > eastern > northeast," the contribution rate of CEI Theil index shows "northeast > eastern > central > western," the CEPC Theil index shows the spatial pattern of "northeast > central > western > eastern," and the contribution rate of CEPC Theil index presents the spatial pattern of "eastern > central > western > northeast." (iii) The dynamic evolution of CEI and CEPC curve presents polarization or multipolar differential phenomenon accompanies with distinct gradient characteristics, the regional difference of agglomeration level in CEI is gradually narrowing, while the CEPC gradually expanding and the dispersion level is increasing, which implies the "intra-regional convergence and inter-regional divergence." Consequently, differential carbon reduction policies have been put forward according to the study findings.


Assuntos
Sequestro de Carbono , Carbono , Agricultura , Carbono/análise , Dióxido de Carbono/análise , China
20.
Artigo em Inglês | MEDLINE | ID: mdl-33668785

RESUMO

The coordinated development of the economy, resources, and environment is a key aspect of sustainable development. China's rapid agricultural modernization has been accompanied by the continuous growth of rural economic aggregate and carbon emissions from the planting industry. However, the quantitative relationship between these two factors and its internal mechanism are not yet fully understood. In this paper, the Intergovernmental Panel on Climate Change (IPCC) method is used to calculate the carbon emissions of the planting industry in China from 1998-2019. Based on this, the Tapio decoupling analysis model was constructed to study the decoupling relationship between economic development and carbon emissions of the planting industry in China from 1998-2019 and the associated spatial and temporal evolution patterns. The effect of the complete decomposition model (without residuals), in terms of carbon emissions from the planting industry, on the process of economic development and its transmission mechanism are introduced. The results show that: (1) The carbon emissions of the planting industry in China increased with the economic development occurring from 1998-2005, where agricultural economic development was highly dependent on resource factors and the environment. The growth trend of carbon emissions of the planting industry slowed from 2006 to 2019, while economic development has gradually realized the decoupling of carbon emissions from the planting industry. (2) From 1998-2019, in Heilongjiang, Sichuan, and Hunan, the economic development was given priority, showing strong and negative decoupling with carbon emissions from farming. The economic development in most regions were given priority, showing strong decoupling with carbon emissions from farming. Up to 2019, decoupling was observed with a significant trend of spatial agglomeration. (3) Economic scale effects had a positive influence on the carbon emissions of the planting industry, while the technology effect and population effect had an inhibiting influence on the carbon emissions of the planting industry. The key policy implication of this paper is that improvement of the quality of economic development serves as the premise for the transformation of the economic development mode. It is necessary to reasonably regulate the economic growth rate and expansion scale, reduce resource consumption and pollutant emission technology, and to make full use of resources, in order to provide a basis for the formulation of reasonable emission reduction policies. An effective way to realize the sustainable development of the agricultural economy would be to improve the technical efficiency, control the population scale appropriately, and optimize the agricultural industrial structure.


Assuntos
Carbono , Desenvolvimento Econômico , Agricultura , Carbono/análise , Dióxido de Carbono/análise , China
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