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Mater Sci Eng C Mater Biol Appl ; 100: 915-927, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30948128


The nepenthes-inspired slippery liquid-infused surface has led to multiple potentials in biomedical devices' design. This study aims to develop a biomimetic, environmentally-friendly slippery layer of oil-infused 3D printed polydimethylsiloxane with anti-bacterial nanosilver (iPDMS/AgNPs) for wound dressing. The engineered 3D printed iPDMS can cater the different requirements of wounds with antifouling, anti-blood staining, and kill bacteria. iPDMS/AgNPs not only exhibits biocompatibility, against adherence and effective antibacterial activity but also effectively promotes neo-epithelial and granulation tissue formation to accelerate wound healing in vivo. Optimized rheologic parameters were obtained for the 3D printable iPDMS pre-polymerization condition. Scanning electronic micrograph (SEM) and Energy Dispersive Spectrometer (EDS) show a uniform mesh with AgNPs dotted on the printed dressing. No cytotoxicity of iPDMS/AgNPs has been found via cell Counting Kit-8(CCK-8) assay. Meanwhile, the membranes infused with silicon oil effectively prevented from the adherence of the two standard drug-resistant bacteria, Staphylococcus aureus and Escherichia coli (PDMS vs. PDMS+oil, p < 0.05; PDMS+0.5%AgNPs vs. iPDMS+0.5%AgNPs, p < 0.05; PDMS+2.5%AgNPs vs. iPDMS+2.5%AgNPs, p < 0.05). By bacteria co-culture model iPDMS/AgNPs can kill about 80% of Staphylococcus aureus and Escherichia coli. When applied to a full-thickness wound defect model of murine, iPDMS/AgNPs was effective in anti-infection. It also promotes the epithelialization, the granulation tissue formation, and wound healing. These findings demonstrate that iPDMS/AgNPs may have therapeutic promise as an ideal wound dressing shortly.

Antibacterianos/uso terapêutico , Incrustação Biológica , Biomimética , Dimetilpolisiloxanos/farmacologia , Óleos/química , Impressão Tridimensional , Cicatrização/efeitos dos fármacos , Infecção dos Ferimentos/tratamento farmacológico , Animais , Antibacterianos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Escherichia coli/efeitos dos fármacos , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Tecido de Granulação/efeitos dos fármacos , Tecido de Granulação/patologia , Íons , Camundongos Endogâmicos BALB C , Testes de Sensibilidade Microbiana , Nanocompostos/química , Nanocompostos/ultraestrutura , Reologia , Prata/química , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/efeitos dos fármacos , Temperatura Ambiente , Infecção dos Ferimentos/patologia
Adv Healthc Mater ; 7(23): e1800118, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30345648


Targeted therapy can improve the accuracy of diagnosis and treatment in the field of cancer management. Cellular surface engineering can enhance cell functions via mounting functional molecules onto cellular membranes. A novel amphiphilic hyperbranched polymer (AHP) conjugated with oleic acid (OA) and tumor-targeted ligand folic acid (FA) is employed. The lipophilic chain can self-assemble and infuse with the cytomembrane of bone marrow mesenchymal stem cells (BMSCs) with the end of FA left on the outside for targeting. The polymer tailored BMSCs can enhance tumor tropism in gastric cancer. BMSCs are characterized by the low immunogenicity and tumor tropism, which makes them promising targeting carriers. Regarding the integrated advantages of these two vectors, it is demonstrated that the functional amphiphilic AHP-OA-FA enhances the tumor tropism of BMSCs. Flow cytometry, standard MTT assay, and wound-healing assay show that AHP-OA-FA has no influence on CD expression, proliferative capacity, and cell motility of BMSCs, respectively. Furthermore, in vitro transwell assay and ex vivo fluorescence image verify that AHP-OA-FA enhances tumor tropism of BMSCs compared to BMSCs and AHP-OA-Rhodamine B-BMSCs. Finally, histological analysis demonstrates that AHP-OA-FA causes no damage to major organs. The results of this study suggest that living BMSCs self-assembled with a polymer might be a promising vehicle for targeted delivery to cancer cells.

Células-Tronco Mesenquimais/metabolismo , Polímeros/química , Animais , Células da Medula Óssea/citologia , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ácido Fólico/química , Ácido Fólico/farmacologia , Humanos , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/citologia , Camundongos Nus , Neoplasias/metabolismo , Neoplasias/patologia , Ácido Oleico/química , Ácido Oleico/farmacologia , Imagem Óptica , Ratos , Ratos Sprague-Dawley , Rodaminas/química
Bioact Mater ; 3(2): 144-156, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29744452


3D printing, an additive manufacturing based technology for precise 3D construction, is currently widely employed to enhance applicability and function of cell laden scaffolds. Research on novel compatible biomaterials for bioprinting exhibiting fast crosslinking properties is an essential prerequisite toward advancing 3D printing applications in tissue engineering. Printability to improve fabrication process and cell encapsulation are two of the main factors to be considered in development of 3D bioprinting. Other important factors include but are not limited to printing fidelity, stability, crosslinking time, biocompatibility, cell encapsulation and proliferation, shear-thinning properties, and mechanical properties such as mechanical strength and elasticity. In this review, we recite recent promising advances in bioink development as well as bioprinting methods. Also, an effort has been made to include studies with diverse types of crosslinking methods such as photo, chemical and ultraviolet (UV). We also propose the challenges and future outlook of 3D bioprinting application in medical sciences and discuss the high performance bioinks.

Burns Trauma ; 5: 4, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28127573


Scars are a natural and unavoidable result from most wound repair procedures and the body's physiological healing response. However, they scars can cause considerable functional impairment and emotional and social distress. There are different forms of treatments that have been adopted to manage or eliminate scar formation. This review covers the latest research in the past decade on using either natural agents or synthetic biomaterials in treatments for scar reduction.