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1.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 34(3): 311-314, 2022 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-35574752

RESUMO

With the development of medical technology, the fixation method of the oral duct is constantly updated, and the selection of a relatively safe, effective, simple and fast fixation method of the oral duct has been widely concerned. However, the use of traditional 3M I-shaped tape fixation needs to be cut, which wastes time and easily leads to the outward displacement of the tracheal tube, and is easy to be soaked by oral secretions, resulting in facial skin damage. Therefore, the medical staffs of the department of critical care medicine of Hengshui People's Hospital designed a self-made tracheal catheter fixing band (composed of the main structure, the fixed band, the internal adjusting structure and the internal structure of the fixed block), and obtained the national utility model patent (ZL 2018 2 0508681.6). The inner side of the fixing band is fixed with a spongy body, which can absorb the secretions around the mouth to avoid the moist condition around the mouth and cheek skin. Meanwhile, the endotracheal catheter is fixed with the help of the card slot, hinge and other structures, which can fully ensure the fixation effect. A total of 80 patients undergoing airway intubation were admitted to the department of critical care medicine of our hospital from October 2020 to September 2021. They were divided into observation group and control group according to intubation time (single number and double number), with 40 patients in each group. The observation group was fixed with self-made tracheal catheter fixation band. Through evidence-based practice path, relevant literatures at home and abroad were searched for clinical practice basis, and the practice plan was formulated and implemented. The control group was fixed with 3M tape + inch tape according to the traditional method. The fixation of tracheal tube and the degree of facial skin injury were compared between the two groups. All patients were included in the final analysis without shedding cases. Severe catheter displacement occurred in 3 patients (7.5%) in the control group, and no severe catheter displacement occurred in the observation group. The incidence of facial skin injury in the observation group was significantly lower than that in the control group [25.0% (10/40) vs. 55.0% (22/40), P < 0.05]. Moreover, the fixation time of the observation group was significantly shorter than that of the control group (minute: 12.11±1.69 vs 17.59±1.27, P < 0.05). The application of self-made tracheal catheter fixation band can shorten the fixation time of tracheal catheter and reduce the incidence of unplanned endotracheal extubation (UEE) and facial skin injury, which is worthy of clinical promotion and application.

3.
Artigo em Inglês | MEDLINE | ID: mdl-35362384

RESUMO

BACKGROUND: Ovarian cancer (OVCA) has unique epigenetic alterations and defects in homologous recombination (HR). Despite initial sensitivity to platinum-based chemotherapy, HR dysfunctional tumors eventually acquire drug resistance. Fanconi anemia (FA) is characterized by bone marrow failure (BMF) and a reduced ability to eradicate DNA interstrand cross-links (ICL). However, the mechanism of chemoresistance mediated by FANCI was unclear in OVCA. OBJECTIVE: We explore to identify whether FANCI was involved in chemoresistance in OVCA. METHODS: FANCI expression and epigenetic alterations were analyzed, respectively, using TIMER and cBioPortal. The correlation between FANCI expression and the survival of OVCA patients was analyzed using Kaplan-Meier Plotter, GSE63885 and TCGA-OVCA database. FANCI expression in OVCA was detected by immunohistochemistry. Cell proliferation, migration, and invasion in FANCI inhibiting cells were assessed by CCK8 and Transwell. Apoptosis and DNA damage were examined by flow cytometry and immunofluorescence. Meanwhile, the activity of caspase 3/7 was detected by Caspase-Glo® 3/7 kit. In addition, the expression of FANCI, γH2AX, and apoptosis effectors was examined by western blot. RESULTS: FANCI has copy number variations (CNVs) in OVCA. The high expression of FANCI in OVCA patients was associated with poor survival. Moreover, FANCI expression was correlated with the response to chemotherapy in OVCA. FANCI expression in OVCA cells was induced by carboplatin in a time-dependent manner. Silencing of FANCI had no effect on cell proliferation, but it hindered OVCA cell migration and invasion. Mechanically, knockdown of FANCI enhanced DNA damage induced apoptosis through CHK1/2-P53-P21 pathway. CONCLUSION: FANCI may be a potential therapeutic target for OVCA patients.

5.
J Investig Med ; 70(3): 844-852, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34872934

RESUMO

The great value of home nursing services in the treatment of ailments in elderly patients has attracted increasing attention. This study describes a new mobile internet-based home nursing service system and investigates the reasons for its use among elderly patients. 520 cases of mobile internet-based home nursing services were investigated. The proportion of major reasons to use mobile internet-based home nursing services among the elderly was analyzed and the satisfaction rate was investigated. The constituent ratios of nursing care for pressure ulcers, peripherally inserted central catheter (PICC), subcutaneous injection, general stoma care, psychological care, and intramuscular injection were 61.35%, 28.85%, 6.15%, 1.92%, 1.35%, and 0.38%, respectively. The satisfaction rate with mobile internet-based home nursing services among elderly patients was 100%. Considering the demand for home nursing services for elderly patients, this is the first time that a new mobile internet-based home nursing service has been applied to provide home nursing services to elderly patients and meet their home nursing service needs. Treatment for pressure ulcers, PICC, subcutaneous injection, general stoma care, psychological care, and intramuscular injection were found to be the main reasons to use mobile internet-based home nursing services among the elderly. The new mobile internet-based home nursing service system provides convenient home nursing services to elderly patients and ensures that they get equal rights in home nursing. The results provide basis for healthcare policy makers to formulate new home nursing policies for elderly patients.

7.
Hum Brain Mapp ; 43(6): 1917-1929, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-34967488

RESUMO

Temporal lobe epilepsy (TLE) is a network disorder with a high incidence of memory impairment. Memory processing ability highly depends on the dynamic coordination between distinct modules within the hippocampal network. Here, we investigate the relationship between memory phenotypes and modular alterations of dynamic functional connectivity (FC) in the hippocampal network in TLE patients. Then, 31 healthy controls and 66 TLE patients with hippocampal sclerosis were recruited. The patients were classified into memory-intact (MI, 35 cases) group and memory-deficit (MD, 31 cases) group, each based on individual's Wechsler Memory Scale-Revised score. The sliding-windows approach and graph theory analysis were used to analyze the hippocampal network based on resting state functional magnetic resonance imaging. Temporal properties and modular metrics were calculated. Two discrete and switchable states were revealed: a high modularized state (State I) and a low modularized state (State II), which corresponded to either anterior or posterior hippocampal network dominated pattern. TLE was prone to drive less State I but more State II, and the tendency was more obvious in TLE-MD. Additionally, TLE-MD showed more widespread alterations of modular properties compared with TLE-MI across two states. Furthermore, the dynamic modularity features had unique superiority in discriminating TLE-MD from TLE-MI. These findings demonstrated that state transitions and modular function of dissociable hippocampal networks were altered in TLE and more importantly, they could reflect different memory phenotypes. The trend revealed potential values of dynamic FC in elucidating the mechanism underlying memory impairments in TLE.


Assuntos
Epilepsia do Lobo Temporal , Hipocampo/diagnóstico por imagem , Humanos , Memória , Transtornos da Memória/diagnóstico por imagem , Transtornos da Memória/etiologia , Fenótipo
8.
Int J Biol Macromol ; 195: 294-301, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34914907

RESUMO

Reactive oxygen species (ROS) for treating bacterial infection is an alternative strategy to overcome the drawbacks such as bacterial resistance of commonly used antibiotics. Nanocatalysts have been proved highly effective in regulating intracellular ROS level due to their intrinsic enzymes-mimicking ability. Herein, we prepared a carbon-based nanozyme doped with copper atoms with peroxidase mimetic activity to catalyze the decomposition of bio-safety dosage of H2O2 to highly reactive OH radicals for antibacterial treatment. Furthermore, we designed the thermo-responsive nanogels consisting of bacterial cellulose nanowhiskers as the carrier of the nanozyme. The obtained nanogels displayed remarkable intelligent response to temperature change with sol-gel transition temperature of ~33 °C and in situ gel forming ability. Moreover, the nanogels exhibited excellent biocompatibility in vitro, along with remarkable antibacterial efficacy which could inactivate 6.36 log of S. aureus and 6.01 log of E. coli in 3 h, respectively. The findings provide a novel strategy for advancing the development of nanocatalysts-based responsive biomaterials for treating bacterial infections.


Assuntos
Antibacterianos/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Nanogéis/química , Antibacterianos/farmacologia , Materiais Biocompatíveis , Catálise , Celulose , Escherichia coli , Peróxido de Hidrogênio , Testes de Sensibilidade Microbiana , Nanopartículas , Espécies Reativas de Oxigênio/química , Staphylococcus aureus
9.
Psychophysiology ; 59(1): e13944, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34553377

RESUMO

Recent studies that used adult faces as the baseline have revealed that attentional bias toward infant faces is the strongest for neutral expressions than for happy and sad expressions. However, the time course of the strongest attentional bias toward infant neutral expressions is unclear. To clarify this time course, we combined a behavioral dot-probe task with electrophysiological event-related potentials (ERPs) to measure adults' responses to infant and adult faces with happy, neutral, and sad expressions derived from the same face. The results indicated that compared with the corresponding expressions in adult faces, attentional bias toward infant faces with various expressions resulted in different patterns during rapid and prolonged attention stages. In particular, first, neutral expressions in infant faces elicited greater behavioral attentional bias and P1 responses than happy and sad ones did. Second, sad expressions in infant faces elicited greater N170 responses than neutral and happy ones did; notably, sad expressions elicited greater N170 responses in the left hemisphere in women than in men. Third, late positive potential (LPP) responses were greater for infant faces than for adult faces under each expression condition. Thus, we propose a three-stage model of attentional allocation patterns that reveals the time course of attentional bias toward infant faces with various expressions. This model highlights the prominent role of neutral facial expressions in the attentional bias toward infant faces.


Assuntos
Viés de Atenção/fisiologia , Potenciais Evocados/fisiologia , Reconhecimento Facial/fisiologia , Adulto , Eletrofisiologia , Feminino , Humanos , Lactente , Masculino , Adulto Jovem
10.
Curr Med Imaging ; 2021 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-34872481

RESUMO

OBJECTIVE: To investigate the role of whole-brain volume computed tomography (CT) perfusion in assessing early ischemic cerebrovascular diseases. MATERIALS AND METHODS: Seventy-two patients with early ischemic cerebrovascular diseases who had undergone routine CT scan and 320-row volume CT whole-brain perfusion imaging within 8 h after admission were retrospectively enrolled in this one-center case-sectional study. The perfusion parameters of cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), time to peak (TTP), and dynamic CT angiogram (4D-CTA) were obtained and analyzed. RESULTS: Among 72 patients, 29 cases with 37 cerebral ischemic lesions were found in plain CT scan, whereas 51 cases with 76 lesions were found in whole-brain CT perfusion, with 30.6% more patients being detected. The CBF value was significantly lower in the abnormal than normal corresponding perfusion area in the healthy hemisphere (P<0.05), while the MTT and TTP values were significantly higher in the abnormal than the normal corresponding area (P<0.05). 4D-CTA image suggested that 59 cases had different degrees of stenosis or occlusion, including 11 mild, 18 moderate, 21 severe, and 9 occlusive cases. Four-D-CTA imaging could detect significantly (P<0.05) more patients with abnormal perfusion in severe cerebral vascular stenosis or occlusion than those with no, mild or moderate stenosis (93.33% vs. 16.67%) (P<0.05). The stenosis of intracranial and carotid arteries was positively correlated with MTT and TTP values (P<0.05). CONCLUSION: Whole-brain volume CT angiography can comprehensively display early cerebral ischemic lesions, cerebral blood perfusion status, and cerebral vascular stenosis, providing valuable information for early detection of ischemic cerebral diseases and appropriate treatment planning.

11.
PLoS Pathog ; 17(12): e1010071, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34882744

RESUMO

Citrus Huanglongbing (HLB), also known as citrus greening, is one of the most devastating citrus diseases worldwide. Candidatus Liberibacter asiaticus (CLas) is the most prevalent strain associated with HLB, which is yet to be cultured in vitro. None of the commercial citrus cultivars are resistant to HLB. The pathosystem of Ca. Liberibacter is complex and remains a mystery. In this review, we focus on the recent progress in genomic research on the pathogen, the interaction of host and CLas, and the influence of CLas infection on the transcripts, proteins, and metabolism of the host. We have also focused on the identification of candidate genes for CLas pathogenicity or the improvements of HLB tolerance in citrus. In the end, we propose potentially promising areas for mechanistic studies of CLas pathogenicity, defense regulators, and genetic improvement for HLB tolerance/resistance in the future.


Assuntos
Citrus/microbiologia , Produtos Agrícolas/microbiologia , Liberibacter/genética , Doenças das Plantas/genética
12.
ACS Appl Mater Interfaces ; 13(49): 58838-58847, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34851081

RESUMO

The polarization curve is the most important profile to evaluate the performance of proton-exchange membrane fuel cells (PEMFCs). To explore the important thermodynamic parameters and their correlation with the composition, fabrication, and operational settings, a comprehensive data set consisting of 446 polarization curves from 191 perfluorosulfonate and 255 sulfonated hydrocarbon-based PEMs is collected. Then, a Markov chain Monte Carlo simulation within the Bayesian frame provides higher than 93% confidence to extract six important thermodynamic parameters including open-circuit potential, the transfer coefficient, the current loss, the reference exchange current density, the internal resistance, and the limiting current density. An extreme gradient boosting algorithm affords a mean determinative coefficient of 0.89 to predict the whole polarization curve and a confidence of 94% to predict the peak power density (PPD). Both approaches to explore the polarization curve for PEMFCs show good robustness in the blind test. Overall, the PPD is positively correlated with the ion-exchange capacity of the polymer, operational temperature, and humidity and is negatively affected by internal resistance, membrane thickness, and the loading of the catalyst. The flow rate of fuels can effectively enhance them, while the increase of catalyst loading or fuel concentration shows deleterious impacts.

13.
Sci Prog ; 104(4): 368504211058554, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34851207

RESUMO

CASE SUMMARY: A patient who underwent mechanical aortic and mitral valve replacement developed three paravalvular leaks 10 months later. We located the tracks by puncturing the apex cordis under transoesophageal echocardiography guidance alone and puncturing the femoral artery guided by fluoroscopy. Three paravalvular leaks were occluded with a hybridization method simultaneously. The patient was followed up for 24 months and maintained a good condition. CONCLUSION: Multiple paravalvular leaks after double valve replacement can be occluded in patients by the use of different approaches under echocardiographic guidance alone.


Assuntos
Implante de Prótese de Valva Cardíaca , Próteses Valvulares Cardíacas , Cateterismo Cardíaco , Ecocardiografia , Ecocardiografia Transesofagiana , Humanos
14.
J Int Med Res ; 49(12): 3000605211068312, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34939869

RESUMO

OBJECTIVE: The concurrence of chronic obstructive pulmonary disease (COPD) and obstructive sleep apnoea syndrome (OSAS) is defined as overlap syndrome (OS), but investigations into predictors of OS in patients with COPD remain limited. Here, potential markers of OS in patients with COPD were investigated, and results of intubation were compared between patients with COPD only or OS. METHODS: This retrospective study included patients with COPD who were divided according to OS diagnosis: COPD only (COPD group) or OS (OS group). RESULTS: Among 206 patients with COPD, 120 were diagnosed with OS. Mean body mass index (BMI) was significantly higher in the OS versus COPD group (28.95 ± 2.96 versus 23.84 ± 4.06, respectively). Receiver operating characteristic curve analyses revealed that BMI was associated with OS (area under the curve, 0.835). The rate of invasive intubation within 48 h was lower in the OS versus COPD group (9.2% versus 20.9%, respectively), and the duration of noninvasive ventilation was longer in the OS versus COPD group. CONCLUSIONS: BMI may be a predictor of OS in patients with COPD. The duration of noninvasive ventilation was longer in patients with OS than in patients with COPD alone.


Assuntos
Ventilação não Invasiva , Doença Pulmonar Obstrutiva Crônica , Apneia Obstrutiva do Sono , Humanos , Intubação Intratraqueal , Doença Pulmonar Obstrutiva Crônica/complicações , Doença Pulmonar Obstrutiva Crônica/terapia , Respiração Artificial , Estudos Retrospectivos , Apneia Obstrutiva do Sono/complicações , Apneia Obstrutiva do Sono/terapia
15.
Ther Adv Neurol Disord ; 14: 17562864211050027, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34671424

RESUMO

BACKGROUND: Malformation of cortical development (MCD) is one of the most common causes of pharmacoresistant epilepsy. Improving the knowledge of antiseizure medications (ASMs) treatment response in epileptic patients with MCD is crucial for optimal treatment options, either pharmacological therapy or non-pharmacological intervention. AIM: To investigate the patterns of medical treatment outcome and the predictors for seizure freedom (SF) with ASM regimens in epilepsy caused by MCD. METHODS: The epileptic patients with MCD were consecutively enrolled from March 2013 to June 2019. SF was defined as no seizures for at least 12 months or three times the longest pretreatment inter-seizure interval, whichever was longer. Outcomes were classified into three patterns: pattern A: patients achieved SF at one point and remained so throughout follow-up; pattern B: patients' seizures fluctuated between periods of SF and relapse; pattern C: SF never attained. The terminal SF was defined if the patients remained SF at the last follow-up visit. RESULTS: A total of 164 epileptic patients with MCD were included. Pattern A was observed in 22, pattern B in 42, and pattern C in 100 patients. SF was ever achieved in 64 (pattern A and B) patients. Twenty-nine patients had terminal SF after a median follow-up time of 4.3 years. With continuing ASM treatment, seizure relapse risk was very low after a 5-year seizure-free period. The pretreatment seizure frequency was the only independent predictor for pattern A and seizure relapse. Sodium channel blockers monotherapy (33.8%) was more effective than levetiracetam (4.5%) in rendering SF in the initial ASM regimen. CONCLUSION: Medical treatment can be successful in a minority of epileptic patients with MCD, and pretreatment seizure frequency helps to predict the treatment outcome. An unequal efficacy of ASMs in epilepsy caused by MCD suggests etiological evaluation is vital in the management of focal epilepsy.

16.
Mitochondrial DNA B Resour ; 6(11): 3117-3118, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34651074

RESUMO

Carex agglomerata C. B. Clarke is a sedge with excellent ornamental characters, it is an important ecosystem stabilizer. Here we report the complete chloroplast genome of C. agglomerata to provide a foundation for further phylogenetic studies on the Cyperaceae. The chloroplast (cp) genome is 184,157 bp in size and consists of a large single-copy (LSC) region 106,654 bp in length, a small single-copy (SSC) region of 36,099 bp, two inverted repeats (IR) regions each 20,702 bp. The total GC content of the cp genome is 33.9% with the LSC, SSC, and IR regions 32, 32.5, and 42.9%, respectively. The cp genome contains 128 genes, including 80 protein-coding, 40 tRNA, and eight rRNA genes. The phylogenetic analysis showed C. agglomerata is in a clade with Carex neurocarpa Maxim and Carex siderosticta Hance. This study provides a basis for further phylogenetic studies of Carex.

17.
JAMA Netw Open ; 4(10): e2128217, 2021 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-34609494

RESUMO

Importance: Few studies have examined the clinicopathological characteristics and prognoses of patients with hepatoid adenocarcinoma of the stomach (HAS). Objective: To explore the clinicopathological characteristics and prognoses of patients with HAS and develop a nomogram to predict overall survival (OS). Design, Setting, and Participants: This prognostic study involved a retrospective analysis of data from 315 patients who received a diagnosis of primary HAS between April 1, 2004, and December 31, 2019, at 14 centers in China. Main Outcomes and Measures: OS and prognostic factors. Patients were randomly assigned to a derivation cohort (n = 220) and a validation cohort (n = 95). A nomogram was developed based on independent prognostic factors identified through a multivariable Cox mixed-effects model. Results: Among 315 patients with HAS (mean [SD] age, 61.9 [10.2] years; 240 men [76.2%]), 137 patients had simple HAS (defined as the presence of histologically contained hepatoid differentiation areas only), and 178 patients had mixed HAS (defined as the presence of hepatoid differentiation areas plus common adenocarcinoma areas). Patients with simple HAS had a higher median preoperative α-fetoprotein level than those with mixed HAS (195.9 ng/mL vs 48.9 ng/mL, respectively; P < .001) and a higher rate of preoperative liver metastasis (23 of 137 patients [16.8%] vs 11 of 178 patients [6.2%]; P = .003). The 3-year OS rates of patients with simple vs mixed HAS were comparable (56.0% vs 60.0%; log-rank P = .98). A multivariable Cox analysis of the derivation cohort found that the presence of perineural invasion (hazard ratio [HR], 2.13; 95% CI, 1.27-3.55; P = .009), preoperative carcinoembryonic antigen levels of 5 ng/mL or greater (HR, 1.72; 95% CI, 1.08-2.74; P = .03), and pathological node category 3b (HR, 3.72; 95% CI, 1.34-10.32; P = .01) were independent risk factors for worse OS. Based on these factors, a nomogram to predict postoperative OS was developed. The concordance indices of the nomogram (derivation cohort: 0.72 [95% CI, 0.66-0.78]; validation cohort: 0.72 [95% CI, 0.63-0.81]; whole cohort: 0.71 [95% CI, 0.66-0.76]) were higher than those derived using the American Joint Committee on Cancer's AJCC Cancer Staging Manual (8th edition) pathological tumor-node-metastasis (pTNM) staging system (derivation cohort: 0.63 [95% CI, 0.57-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]) and those derived using a clinical model that included pTNM stage and receipt of adjuvant chemotherapy (derivation cohort: 0.64 [95% CI, 0.58-0.69]; validation cohort: 0.65 [95% CI, 0.56-0.75]; whole cohort: 0.64 [95% CI, 0.59-0.69]). Based on the nomogram cutoff of 10 points, the whole cohort was divided into high-risk and low-risk groups. The 3-year OS rate of patients in the high-risk group was significantly lower than that of patients in the low-risk group (29.7% vs 75.9%, respectively; log-rank P < .001), and the 3-year prognosis of high-risk and low-risk groups could be further distinguished into pTNM stage I to II (33.3% vs 80.2%; exact log-rank P = .15), stage III (34.3% vs 71.3%; log-rank P < .001), and stage IV (15.5% vs 70.3%; log-rank P = .009). Conclusions and Relevance: This study found that perineural invasion, preoperative carcinoembryonic antigen levels of 5 ng/mL or greater, and pathological node category 3b were independent risk factors associated with worse OS. An individualized nomogram was developed to predict OS among patients with HAS. This nomogram had good prognostic value and may be useful as a supplement to the current American Joint Committee on Cancer TNM staging system.


Assuntos
Prognóstico , Neoplasias Gástricas/patologia , Adenocarcinoma/epidemiologia , Adenocarcinoma/patologia , Idoso , China/epidemiologia , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Modelos de Riscos Proporcionais , Fatores de Risco , Neoplasias Gástricas/classificação , Neoplasias Gástricas/epidemiologia
18.
Transl Psychiatry ; 11(1): 542, 2021 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-34671017

RESUMO

The pathogenesis of Alzheimer's disease (AD) involves multiple cell types including endothelial cells, glia, and neurons. It suggests that therapy against single target in single cell type may not be sufficient to treat AD and therapies with protective effects in multiple cell types may be more effective. Here, we comprehensively investigated the effects of bilobalide on neuroinflammation and Aß degrading enzymes in AD cell model and mouse model. We find that bilobalide inhibits Aß-induced and STAT3-dependent expression of TNF-α, IL-1ß, and IL-6 in primary astrocyte culture. Bilobalide also induces robust expression of Aß degrading enzymes like NEP, IDE, and MMP2 to facilitate astrocyte-mediated Aß clearance. Moreover, bilobalide treatment of astrocyte rescues neuronal deficiency in co-cultured APP/PS1 neurons. Most importantly, bilobalide reduces amyloid and inflammation in AD mouse brain. Taken together, the protective effects of bilobalide in in vitro cultures were fully recapitulated in in vivo AD mouse model. Our study supports that bilobalide has therapeutic potential for AD treatment.


Assuntos
Doença de Alzheimer , Bilobalídeos , Doença de Alzheimer/tratamento farmacológico , Peptídeos beta-Amiloides , Animais , Astrócitos , Células Endoteliais , Inflamação/tratamento farmacológico , Camundongos , Neurônios
19.
Cancer Control ; 28: 10732748211041881, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34569311

RESUMO

BACKGROUND: Although Helicobacter pylori (Hp) as high risk factor for gastric cancer have been investigated from human trial, present data is inadequate to explain the effect of Hp on the changes of metabolic phenotype of gastric cancer in different stages. PURPOSE: Herein, plasma of human superficial gastritis (Hp negative and positive), early gastric cancer and advanced gastric cancer analyzed by UPLC-HDMS metabolomics can not only reveal metabolic phenotype changes in patients with gastric cancer of different degrees (30 Hp negative, 30 Hp positive, 20 early gastric cancer patients, and 10 advanced gastric cancer patients), but also auxiliarily diagnose gastric cancer. RESULTS: Combined with multivariate statistical analysis, the results represented biomarkers different from Hp negative, Hp positive, and the alterations of metabolic phenotype of gastric cancer patients. Forty-three metabolites are involved in amino acid metabolism, and lipid and fatty acid metabolism pathways in the process of cancer occurrence, especially 2 biomarkers glycerophosphocholine and neopterin, were screened in this study. Neopterin was consistently increased with gastric cancer progression and glycerophosphocholine tended to consistently decrease from Hp negative to advanced gastric cancer. CONCLUSION: This method could be used for the development of rapid targeted methods for biomarker identification and a potential diagnosis of gastric cancer.


Assuntos
Gastrite/diagnóstico , Gastrite/patologia , Helicobacter pylori/isolamento & purificação , Metabolômica/métodos , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/patologia , Biomarcadores Tumorais , Diagnóstico Diferencial , Humanos , Estadiamento de Neoplasias , Neopterina/sangue , Fenótipo , Análise de Componente Principal
20.
Seizure ; 91: 484-489, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34343860

RESUMO

PURPOSE: We aimed to investigate the differences of white matter (WM) between the focal cortical dysplasia (FCD) patients with drug-resistant epilepsy and those with drug-responsive epilepsy. METHODS: Thirty epileptic patients with MRI-identified or histologically proven FCD were consecutively enrolled. Fractional anisotropy (FA) and mean diffusivity (MD) of the ipsilateral perilesional WM and contralateral homotopic WM layer masks were computed and corrected by the FA/MD of the corresponding hemispheric WM. The difference was evaluated using paired t-tests. The FA, MD and volumes of hemispheric WM and corpus callosum were also calculated. RESULTS: Patients with drug-resistant epilepsy showed significantly decreased FA and increased MD among ipsilateral perilesional WM layer 1 and 2, while patients with drug-responsive epilepsy showed decreased FA in only ipsilateral perilesional WM layer l, compared to remaining ipsilateral perilesional WM layers and contralateral layers 1 through 6. The integrity and volumes of the hemispheric WM and corpus callosum were similar between the two groups. CONCLUSION: We demonstrated that the WM microstructural alterations differed between epileptic patients with FCD according to their antiepileptic drug responses. More extensive perilesional WM abnormality is observed in patients with drug-resistant epilepsy related to FCD.


Assuntos
Epilepsia , Malformações do Desenvolvimento Cortical , Preparações Farmacêuticas , Substância Branca , Imagem de Tensor de Difusão , Epilepsia/complicações , Epilepsia/diagnóstico por imagem , Epilepsia/tratamento farmacológico , Humanos , Imageamento por Ressonância Magnética , Malformações do Desenvolvimento Cortical/complicações , Malformações do Desenvolvimento Cortical/diagnóstico por imagem , Substância Branca/diagnóstico por imagem
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