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1.
BMC Cardiovasc Disord ; 22(1): 352, 2022 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-35922759

RESUMO

BACKGROUND: The KODEX-EPD system is a novel, dielectric three-dimensional mapping system. We aim to illustrate the feasibility, safety, and outcomes of ablation using the KODEX-EPD system. METHODS: A total of 272 patients with supraventricular arrhythmias were enrolled and underwent catheter ablation using the KODEX-EPD system from October 2020 to July 2021. The feasibility, safety, and ablation outcomes were analyzed. RESULTS: Of the enrolled patients, 15 (5.4%) had atrial tachycardia (AT), 88 (31.4%) had atrioventricular reentrant tachycardia (AVRT), 141 (50.4%) had atrioventricular nodal reentrant tachycardia (AVNRT), 34 (12.1%) had atrial fibrillation (AF), and 9 (3.2%) had atrial flutter (AFL). All AF patients included were first-do-pulmonary vein isolation (PVI); there were 26 paroxysmal AF and 8 persistent AF. All patients achieved immediate success of ablation. The mean follow-up duration was 11.8 ± 2.4 months. One patient (1.1%) in the AVRT subgroup and two patients (1.4%) in the AVNRT subgroup experienced recurrence. When considering a three-month blanking time, the estimated freedom of AF at one-year post-ablation with and without AADs was 75.7% and 70.4%, respectively. The Kaplan-Meier analysis showed no significant difference in the overall AF recurrence (log-rank; P = 0.931) or AAD-free AF recurrence (log-rank; P = 0.841) between RFCA and cryoablation. One patient had mild pulmonary embolism. None of the patients died or had a cerebrovascular event in the periprocedural period. CONCLUSIONS: This retrospective, two-center study demonstrated that catheter ablation of supraventricular arrhythmias using the KODEX-EPD system is feasible, safe, and effective. Trial registration Retrospectively registered.


Assuntos
Técnicas de Ablação , Ablação por Cateter , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Flutter Atrial/diagnóstico , Flutter Atrial/cirurgia , Ablação por Cateter/efeitos adversos , China , Humanos , Veias Pulmonares/cirurgia , Recidiva , Estudos Retrospectivos , Taquicardia por Reentrada no Nó Atrioventricular/diagnóstico , Taquicardia por Reentrada no Nó Atrioventricular/cirurgia , Taquicardia Supraventricular/diagnóstico , Taquicardia Supraventricular/cirurgia , Resultado do Tratamento
2.
Bioresour Technol ; : 127717, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35926559

RESUMO

Modified biochar is a feasible adsorbent to solve cadmium pollution in water. However, few studies could elucidate the mechanism of cadmium adsorption by biochar from a molecular perspective. Furthermore, traditional modification methods are costly and have the risk of secondary contamination. Hence, several environmentally friendly sodium salts were used to modify the water chestnut shell-based biochar and employ it in the Cd2+ adsorption in this work. The modification of sodium salt could effectively improve the specific surface area and aromaticity of biochar. Na3PO4 modified biochar exhibited the highest Cd2+ adsorption capacity (112.78 mg/g). The adsorption of Cd2+ onto biochar was an endothermic, monolayer, chemisorption process accompanied by intraparticle diffusion. Microscopically, the enhancement of aromatization after modification made Cd2+ more likely to interact with the regions rich in π electrons and lone pair electrons. This study provided a new research perspective and application guidance for heavy metal adsorption on biochar.

3.
BMC Cardiovasc Disord ; 22(1): 326, 2022 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-35869446

RESUMO

BACKGROUND: Percutaneous left atrial appendage (LAA) occlusion is effective for stroke prevention in patients with atrial fibrillation. LAA can have a complex anatomy, such as multiple lobes or a large orifice, which may render it unsuitable for occlusion using regular devices. We aimed to investigate the feasibility, safety, and short-term efficacy of the small-umbrella LAmbre device for morphologically complicated LAA. METHODS: We retrospectively enrolled 129 consecutive patients who underwent LAA occlusion using the LAmbre device; the small-umbrella LAmbre device was used in 30 of these patients. We analyzed patients' characteristics, procedural details, and outcomes. RESULTS: Twenty-two patients (73.3%) had multilobed (≥ 2) LAA. The umbrella of the occluder was anchored in the branch in 9 patients and in the common trunks of branches in 13 patients. The landing zone and orifice diameters were 19.0 ± 4.39 mm and 27.4 ± 3.95 mm, respectively. The sizes of the umbrella and occluder cover were 22.0 ± 3.42 mm and 34.3 ± 2.75 mm, respectively. At 3-month follow-up transesophageal echocardiography in 24 patients, no peri-device residual flow was reported. Device thrombosis was detected in one patient at 3 months and disappeared after 3 months of anticoagulation. Ischemic stroke occurred in one patient; no other adverse events were reported. CONCLUSIONS: Occlusion of morphologically complicated LAA using the small-umbrella LAmbre device was feasible, safe, and effective in patients with atrial fibrillation in this study. This occluder provides an alternative for patients who cannot be treated with regular-sized LAA occlusion devices.


Assuntos
Apêndice Atrial , Fibrilação Atrial , Dispositivo para Oclusão Septal , Acidente Vascular Cerebral , Apêndice Atrial/diagnóstico por imagem , Fibrilação Atrial/complicações , Fibrilação Atrial/diagnóstico , Fibrilação Atrial/cirurgia , Cateterismo Cardíaco/efeitos adversos , Ecocardiografia Transesofagiana/efeitos adversos , Humanos , Estudos Retrospectivos , Dispositivo para Oclusão Septal/efeitos adversos , Acidente Vascular Cerebral/complicações , Acidente Vascular Cerebral/prevenção & controle , Resultado do Tratamento
4.
Eur J Med Res ; 27(1): 130, 2022 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-35879739

RESUMO

BACKGROUND: In recent years, with the increasing incidence of colorectal cancer (CRC) and its high fatality rate, CRC has seized the attention of the world. And liver metastasis, as the main cause of death of CRC, has become the leading cause of treatment failure in CRC, especially metachronous liver metastasis, have caused patients who underwent bowel resection to experience multiple tortures. MAIN BODY: Metachronous liver metastasis has severely affected the quality of life and prognosis of patients. Therefore, in this review, we discuss risk factors for metachronous liver metastasis of CRC, which is the premise for effective intervention for CRC patients who suffer metachronous liver metastasis after undergoing surgery, as well as the signaling pathways associated with CRC. CONCLUSION: The occurrence of metachronous liver metastasis is closely related to histology-based prognostic biomarkers, serum-based biomarkers, tumor microenvironment, pre-metastatic niche, liquid biopsy and tissue-based biomarkers. Further research is required to explore the risk factors associated with liver metastasis of CRC.


Assuntos
Neoplasias Colorretais , Neoplasias Hepáticas , Biomarcadores , Neoplasias Colorretais/patologia , Humanos , Neoplasias Hepáticas/cirurgia , Qualidade de Vida , Estudos Retrospectivos , Fatores de Risco , Microambiente Tumoral
5.
Sci Rep ; 12(1): 12140, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35840643

RESUMO

Human brains experience whole-brain anatomic and functional changes throughout the lifespan. Age-related whole-brain network changes have been studied with functional magnetic resonance imaging (fMRI) to determine their low-frequency spatial and temporal characteristics. However, little is known about age-related changes in whole-brain fast dynamics at the scale of neuronal events. The present study investigated age-related whole-brain dynamics in resting-state electroencephalography (EEG) signals from 73 healthy participants from 6 to 65 years old via characterizing transient neuronal coactivations at a resolution of tens of milliseconds. These uncovered transient patterns suggest fluctuating brain states at different energy levels of global activations. Our results indicate that with increasing age, shorter lifetimes and more occurrences were observed in the brain states that show the global high activations and more consecutive visits to the global highest-activation brain state. There were also reduced transitional steps during consecutive visits to the global lowest-activation brain state. These age-related effects suggest reduced stability and increased fluctuations when visiting high-energy brain states and with a bias toward staying low-energy brain states. These age-related whole-brain dynamics changes are further supported by changes observed in classic alpha and beta power, suggesting its promising applications in examining the effect of normal healthy brain aging, brain development, and brain disease.


Assuntos
Mapeamento Encefálico , Encéfalo , Adolescente , Adulto , Idoso , Encéfalo/diagnóstico por imagem , Encéfalo/fisiologia , Mapeamento Encefálico/métodos , Criança , Eletroencefalografia/métodos , Humanos , Imageamento por Ressonância Magnética/métodos , Pessoa de Meia-Idade , Neurônios , Adulto Jovem
6.
Neuroimage ; 260: 119461, 2022 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-35820583

RESUMO

Spontaneous neural activity in human as assessed with resting-state functional magnetic resonance imaging (fMRI) exhibits brain-wide coordinated patterns in the frequency of  < 0.1 Hz. However, understanding of fast brain-wide networks at the timescales of neuronal events (milliseconds to sub-seconds) and their spatial, spectral, and transitional characteristics remain limited due to the temporal constraints of hemodynamic signals. With milli-second resolution and whole-head coverage, scalp-based electroencephalography (EEG) provides a unique window into brain-wide networks with neuronal-timescale dynamics, shedding light on the organizing principles of brain functions. Using the state-of-the-art signal processing techniques, we reconstructed cortical neural tomography from resting-state EEG and extracted component-based co-activation patterns (cCAPs). These cCAPs revealed brain-wide intrinsic networks and their dynamics, indicating the configuration/reconfiguration of resting human brains into recurring and transitional functional states, which are featured with the prominent spatial phenomena of global patterns and anti-state pairs of co-(de)activations. Rich oscillational structures across a wide frequency band (i.e., 0.6 Hz, 5 Hz, and 10 Hz) were embedded in the nonstationary dynamics of these functional states. We further identified a superstructure that regulated between-state immediate and long-range transitions involving the entire set of identified cCAPs and governed a significant aspect of brain-wide network dynamics. These findings demonstrated how resting-state EEG data can be functionally decomposed using cCAPs to reveal rich dynamic structures of brain-wide human neural activations.

7.
Neuroimage ; 260: 119460, 2022 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-35868615

RESUMO

Brain-wide patterns in resting human brains, as either structured functional connectivity (FC) or recurring brain states, have been widely studied in the neuroimaging literature. In particular, resting-state FCs estimated over windowed timeframe neuroimaging data from sub-minutes to minutes using correlation or blind source separation techniques have reported many brain-wide patterns of significant behavioral and disease correlates. The present pilot study utilized a novel whole-head cap-based high-density diffuse optical tomography (DOT) technology, together with data-driven analysis methods, to investigate recurring transient brain-wide patterns in spontaneous fluctuations of hemodynamic signals at the resolution of single timeframes from thirteen healthy adults in resting conditions. Our results report that a small number, i.e., six, of brain-wide coactivation patterns (CAPs) describe major spatiotemporal dynamics of spontaneous hemodynamic signals recorded by DOT. These CAPs represent recurring brain states, showing spatial topographies of hemispheric symmetry, and exhibit highly anticorrelated pairs. Moreover, a structured transition pattern among the six brain states is identified, where two CAPs with anterior-posterior spatial patterns are significantly involved in transitions among all brain states. Our results further elucidate two brain states of global positive and negative patterns, indicating transient neuronal coactivations and co-deactivations, respectively, over the entire cortex. We demonstrate that these two brain states are responsible for the generation of a subset of peaks and troughs in global signals (GS), supporting the recent reports on neuronal relevance of hemodynamic GS. Collectively, our results suggest that transient neuronal events (i.e., CAPs), global brain activity, and brain-wide structured transitions co-exist in humans and these phenomena are closely related, which extend the observations of similar neuronal events recently reported in animal hemodynamic data. Future studies on the quantitative relationship among these transient events and their relationships to windowed FCs along with larger sample size are needed to understand their changes with behaviors and diseased conditions.

8.
World J Urol ; 2022 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-35869317

RESUMO

BACKGROUND: Although most studies believe that systematic biopsy (SB) and targeted biopsy (TB) should be performed simultaneously in patients with suspected prostate cancer, we believe that patients with the Prostate Imaging-Reporting and Data System (PI-RADS) score of 4/5 may be able to perform TB only. METHODS: We retrospectively analyzed the pathological results of patients undergoing transperineal prostate biopsy with PI-RADS 4 and 5 in our center. We use the data from 2019 to 2020 as the training set to establish the prediction model and the data from 2021 as the verification set to test the effectiveness. Through stepwise logistics regression analysis, we integrate statistically significant clinical factors and establish a model to further predict whether the target area is tumor. RESULTS: The results showed that age (O), total number of lesions (T), histological region (R), PI-RADS score (S), and PSA density (P) were significantly correlated with the results of TB, and the formula was: p = 1/[1 + e^(- 11.387 + 0.058 × O + (- 0.736 × T) + 0.587 × R + 1.574 × S + 7.338 × P)]. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve of the prediction model was 0.840 (95% CI 0.802-0.877), with the optimal threshold of 0.762. And the corresponding specificity and sensitivity were 0.765 and 0.752. In the validation set, the AUC of the prediction model was 0.816 (95% CI 0.759-0.874), which means that it has good prediction efficiency. CONCLUSION: The P.R.O.S.T score can effectively screen PI-RADS 4/5 lesions, which may help physicians shunt patients who need prostate biopsy to reduce unnecessary systematic biopsies.

9.
Chem Commun (Camb) ; 58(60): 8428-8431, 2022 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-35796312

RESUMO

A serial oxygen vacancy (OV) on Nb2O5 is synthesised by H2 calcination, and H-300 exhibited high selectivity and activity for H2O2 (93.4%, 562.5 mmol gcat-1). A volcano relationship is identified between the OV content and performance, which provides an attractive insight into designing electrocatalysts.

10.
Bioinform Adv ; 2(1): vbac033, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35722206

RESUMO

Motivation: Methods for the global measurement of transcript abundance such as microarrays and RNA-Seq generate datasets in which the number of measured features far exceeds the number of observations. Extracting biologically meaningful and experimentally tractable insights from such data therefore requires high-dimensional prediction. Existing sparse linear approaches to this challenge have been stunningly successful, but some important issues remain. These methods can fail to select the correct features, predict poorly relative to non-sparse alternatives or ignore any unknown grouping structures for the features. Results: We propose a method called SuffPCR that yields improved predictions in high-dimensional tasks including regression and classification, especially in the typical context of omics with correlated features. SuffPCR first estimates sparse principal components and then estimates a linear model on the recovered subspace. Because the estimated subspace is sparse in the features, the resulting predictions will depend on only a small subset of genes. SuffPCR works well on a variety of simulated and experimental transcriptomic data, performing nearly optimally when the model assumptions are satisfied. We also demonstrate near-optimal theoretical guarantees. Availability and implementation: Code and raw data are freely available at https://github.com/dajmcdon/suffpcr. Package documentation may be viewed at https://dajmcdon.github.io/suffpcr. Contact: daniel@stat.ubc.ca. Supplementary information: Supplementary data are available at Bioinformatics Advances online.

11.
Artigo em Inglês | MEDLINE | ID: mdl-35725883

RESUMO

The impact of green credit on banks has been widely verified. This paper systematically combs the internal mechanism of the impact of green credit on high-quality sustainable development of banks and studies the relationship between green credit and high-quality sustainable development of banks by using unbalanced panel data of Chinese listed banks from 2007 to 2019. The results show that green credit will significantly promote high-quality sustainable development of banks, which remains valid after controlling for relevant variables and robustness test. The further test results show that the impact of green credit on high-quality sustainable development of banks will be affected by green environment supervision and monetary policy. Green environment supervision and loose monetary policy promote the positive impact of green credit on high-quality sustainable development of banks. The heterogeneity test results show that the impact of green credit on high-quality sustainable development of banks is significantly different among banks with different business scope and different size, and the positive impact of green credit on high-quality sustainable development of small-scale banks and regional banks is more significant. This paper enriches the research of the impact of green credit on banks.

12.
J Cardiol ; 2022 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-35659157

RESUMO

BACKGROUND: A novel dielectric wide-band imaging system with tissue pressure (TP) technology provides real-time contact force (CF) monitoring using non-CF catheters. This study sought to investigate the feasibility, safety, and efficacy of ablation with TP technology. METHODS: Eighty-five patients with supraventricular tachycardia (SVT) were ablated with real-time monitoring of CF by TP technology and compared with 85 patients who underwent ablation with a conventional non-TP approach. Baseline characteristics, procedural data, and TP data were analyzed in the study. Ablation applications in the TP group were then subdivided into good contact and poor contact groups according to the TP level for analysis. RESULTS: The TP group had a significantly shorter procedural time (16.2 ±â€¯6.9 min vs. 19.9 ±â€¯10.0 min, p = 0.033), shorter ablation time (334.6 ±â€¯166.9 s vs. 391.3 ±â€¯195.7 s, p = 0.049), and fewer mean numbers of radiofrequency catheter ablation (RFCA) deliveries (6.2 ±â€¯3.2 vs. 7.6 ±â€¯5.2, p = 0.047) than the non-TP group. The achieved average percentage of TP >3 g was significantly higher in the good-contact group (97.94% vs. 15.48%, p < 0.001). The median impedance decreases during RFCA in the good contact group and poor contact group were 4.10 (0.30-6.88) Ω and 2.60 (-0.05-4.98) Ω at 10 s, 4.45 (0.58-8.25) Ω and 2.88 (0.23-5.70) Ω at 20 s, and 4.67 (1.95-9.08) Ω and 2.97 (-0.26-6.33) Ω at 30 s, respectively (p < 0.05 for comparisons between categories). All patients achieved acute success, and no complications were observed. Two patients in the TP group and one patient in the non-TP group experienced recurrence during follow-up. CONCLUSION: TP-technology guided ablation of SVT is feasible, efficient, and safe.

13.
Bioengineered ; 13(6): 14080-14093, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35730503

RESUMO

Sensorineural hearing loss (SNHL) is a common clinical side effect resulted from the overusing of aminoglycoside antibacterial drugs, such as gentamicin. Oxidative stress is recently evidenced to be an important inducer for SNHL, which is reported to be associated with the knockdown of connexin-43. MiR-106a is recently found as a regulator of connexin-43. The present study aims to investigate whether miR-106a is a vital mediator in the development of SNHL. Firstly, upregulated miR-106a was observed in the peripheral blood sample of SNHL patients. Glucose oxidase (GO) was utilized to induce oxidative injury in isolated rat cochlear marginal cells (MCs), followed by introducing the miR-106a inhibitor. We found that the declined proliferation ability, increased apoptosis, and activated oxidative stress in GO-stimulated MCs were dramatically abolished by the miR-106a inhibitor, accompanied by the upregulation of connexin-43. The targeting correlation between miR-106a and connexin-43 was predicted and confirmed by the dual luciferase gene reporter assay. Furthermore, the regulatory effect of miR-106a inhibitor against the proliferation, apoptosis, and oxidative stress in GO-treated MCs were dramatically abolished by the knockdown of connexin-43. Gentamicin was utilized to establish the SNHL model in rats, followed by the treatments of antagomir-106a and antagomir-106a combined with carbenoxolone, an inhibitor of connexin-43. The alleviated pathological state, reduced apoptosis, and ameliorated oxidative stress in cochlea tissues were observed in antagomir-106a treated SNHL rats, which were dramatically reversed by the co-administration of carbenoxolone. Collectively, miR-106a facilitated the SNHL induced by oxidative stress via targeting connexin-43.


Assuntos
Perda Auditiva Neurossensorial , MicroRNAs , Animais , Antagomirs , Carbenoxolona , Proliferação de Células/genética , Gentamicinas/farmacologia , Perda Auditiva Neurossensorial/genética , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Estresse Oxidativo/genética , Ratos
14.
JACC Clin Electrophysiol ; 8(5): 665-676, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35589180

RESUMO

OBJECTIVES: The purpose of this study was to investigate the spatial distribution of ventricular arrhythmias (VAs) and their relationship with anatomic landmarks in the right ventricular outflow tract (RVOT). BACKGROUND: Although controversy has mainly focused on whether VAs ablated in the RVOT originate above or below the pulmonary sinus, little is known about their actual distribution. METHODS: We performed mapping and ablation in the reconstructed RVOT using intracardiac echocardiography (ICE) and summarized the spatial electroanatomic characteristics of RVOT-VAs. RESULTS: A total of 50 VAs were recruited and were distributed among the 3 subregions: the pulmonary sinuses (19 of 50, 38%), sinus junctions (18 of 50, 36%), and infundibulum (13 of 50, 26%). In total, 70% (35 of 50) of ablation targets were within 10 mm (mean 4.3 ± 2.7 mm) of the pulmonary valve hinge point. An ablation target with both amplitude ≤1.14 mV and duration ≥101.5 milliseconds predicted an origin above the pulmonary sinus with a sensitivity of 84.2% and specificity of 84.4%. For the ablation targets (13 of 50, 26%) located in the infundibulum of the RVOT, 4 were surrounded by trabeculations, whereas only 1 ablation target in the sinus junction abutted the trabeculation (30.8% vs 5.6%). CONCLUSIONS: Ablation targets of RVOT-VAs were mainly distributed around the hinge point of the pulmonary valve and the trabeculation of the infundibulum. ICE can clearly and precisely locate those anatomic landmarks of the RVOT.


Assuntos
Ablação por Cateter , Taquicardia Ventricular , Arritmias Cardíacas , Ecocardiografia , Eletrocardiografia , Humanos , Taquicardia Ventricular/cirurgia
15.
Proc Natl Acad Sci U S A ; 119(19): e2119990119, 2022 05 10.
Artigo em Inglês | MEDLINE | ID: mdl-35522713

RESUMO

Over the years it has been established that SIN1, a key component of mTORC2, could interact with Ras family small GTPases through its Ras-binding domain (RBD). The physical association of Ras and SIN1/mTORC2 could potentially affect both mTORC2 and Ras-ERK pathways. To decipher the precise molecular mechanism of this interaction, we determined the high-resolution structures of HRas/KRas-SIN1 RBD complexes, showing the detailed interaction interface. Mutation of critical interface residues abolished Ras-SIN1 interaction and in SIN1 knockout cells we demonstrated that Ras-SIN1 association promotes SGK1 activity but inhibits insulin-induced ERK activation. With structural comparison and competition fluorescence resonance energy transfer (FRET) assays we showed that HRas-SIN1 RBD association is much weaker than HRas-Raf1 RBD but is slightly stronger than HRas-PI3K RBD interaction, providing a possible explanation for the different outcome of insulin or EGF stimulation. We also found that SIN1 isoform lacking the PH domain binds stronger to Ras than other longer isoforms and the PH domain appears to have an inhibitory effect on Ras-SIN1 binding. In addition, we uncovered a Ras dimerization interface that could be critical for Ras oligomerization. Our results advance our understanding of Ras-SIN1 association and crosstalk between growth factor-stimulated pathways.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Transdução de Sinais , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Proliferação de Células , Alvo Mecanístico do Complexo 2 de Rapamicina/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas ras/metabolismo
16.
Front Plant Sci ; 13: 881055, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35586216

RESUMO

Zinc is an essential micronutrient for plant growth and development, and functions as a cofactor for hundreds of transcription factors and enzymes in numerous biological processes. Zinc deficiency is common abiotic stress resulting in yield loss and quality deterioration of crops, but zinc excess causes toxicity for biological systems. In plants, zinc homeostasis is tightly modulated by zinc transporters and binding compounds that uptake/release, transport, localize, and store zinc, as well as their upstream regulators. Lazarus 1 (LAZ1), a member of DUF300 protein family, functions as transmembrane organic solute transporter in vertebrates. However, the function of LAZ1 in plants is still obscure. In the present study, the ZmLAZ1-4 protein was confirmed to bind to zinc ions by bioinformatic prediction and thermal shift assay. Heterologous expression of ZmLAZ1-4 in the zinc-sensitive yeast mutant, Arabidopsis, and maize significantly facilitated the accumulation of Zn2+ in transgenic lines, respectively. The result of subcellular localization exhibited that ZmLAZ1-4 was localized on the plasma and vacuolar membrane, as well as chloroplast. Moreover, the ZmLAZ1-4 gene was negatively co-expressed with ZmBES1/BZR1-11 gene through co-expression and real-time quantitative PCR analysis. The results of yeast one-hybrid and dual-luciferase assay suggested that ZmBES1/BZR1-11 could bind to ZmLAZ1-4 promoter to inhibit its transcription. All results indicated that ZmLAZ1-4 was a novel zinc transporter on plasma and vacuolar membrane, and transported zinc under negative regulation of the ZmBES1/BZR1-11 transcription factor. The study provides insights into further underlying the mechanism of ZmLAZ1-4 regulating zinc homeostasis.

17.
Artigo em Inglês | MEDLINE | ID: mdl-35412995

RESUMO

Distinguishing bipolar depression (BD) from unipolar depression (UD) based on symptoms only is challenging. Brain functional connectivity (FC), especially dynamic FC, has emerged as a promising approach to identify possible imaging markers for differentiating BD from UD. However, most of such studies utilized conventional FC and group-level statistical comparisons, which may not be sensitive enough to quantify subtle changes in the FC dynamics between BD and UD. In this paper, we present a more effective individualized differentiation model based on machine learning and the whole-brain high-order functional connectivity (HOFC) network. The HOFC, capturing temporal synchronization among the dynamic FC time series, a more complex chronnectome metric compared to the conventional FC, was used to classify 52 BD, 73 UD, and 76 healthy controls (HC). We achieved a satisfactory accuracy (70.40%) in BD vs. UD differentiation. The resultant contributing features revealed the involvement of the coordinated flexible interactions among sensory (e.g., olfaction, vision, and audition), motor, and cognitive systems. Despite sharing common chronnectome of cognitive and affective impairments, BD and UD also demonstrated unique dynamic FC synchronization patterns. UD is more associated with abnormal visual-somatomotor inter-network connections, while BD is more related to impaired ventral attention-frontoparietal inter-network connections. Moreover, we found that the illness duration modulated the BD vs. UD separation, with the differentiation performance hampered by the secondary disease effects. Our findings suggest that BD and UD may have divergent and convergent neural substrates, which further expand our knowledge of the two different mental disorders.

18.
J Med Microbiol ; 71(4)2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35467501

RESUMO

Introduction. Colorectal cancer (CRC) is one of the most common cancers worldwide. Multiple risk factors are involved in CRC development, including age, genetics, lifestyle, diet and environment. Of these, the role of the gut microbiota in cancer biology is increasingly recognized.Hypothesis/Gap Statement. Micro-organisms have been widely detected in stool samples, but few mucosal samples have been detected and sequenced in depth.Aim. Analysis of cultured mucosal bacteria from colorectal cancer and adjacent normal mucosal tissues with metagenomics sequencing.Methodology. Twenty-eight paired tumour and non-tumour tissues from 14 patients undergoing surgery for CRC were analysed. We removed the influence of eukaryotic cells via culture. The composition of mucosal microbiota in intestinal mucosa were detected and analysed with metagenomic sequencing.Results. Compared with non-cultured mucosal sample, 80 % bacteria species could be detected after culture. Moreover, after culture, additional 30 % bacteria could be detected, compared with non-cultured samples. Since after culture it was difficult to estimate the original abundance of microbiome, we focused on the identification of the CRC tissue-specific species. There were 298 bacterial species, which could only be cultured and detected in CRC tissues. Myroides odoratimimus and Cellulophaga baltica could be isolated from all the tumour samples of 14 CRC patients, suggesting that these species may be related to tumour occurrence and development. Further functional analysis indicated that bacteria from CRC tissues showed more active functions, including basic metabolism, signal transduction and survival activities.Conclusion. We used a new method based on culture to implement information on prokaryotic taxa, and related functions, which samples were from colorectal tissues. This method is suitable for removing eukaryotic contamination and detecting micro-organisms from other tissues.


Assuntos
Neoplasias Colorretais , Microbioma Gastrointestinal , Bactérias/genética , Neoplasias Colorretais/microbiologia , Humanos , Mucosa Intestinal/microbiologia , Metagenômica
19.
Artigo em Chinês | MEDLINE | ID: mdl-35483686

RESUMO

Objective:To analyze the objective test results of the pure-tone audiogram (PTA), extended high-frequency audiometry (EHFA), distortion product otoacoustic emission (DPOAE), auditory brainstem response(ABR), and electrocochleogram (ECochG) in patients with unilateral acute tinnitus, summarize their characteristics and explore their clinical application value in hidden hearing loss. Methods:PTA, DPOAE, ABR, and ECochG tests were performed in 33 patients with unilateral acute tinnitus as the chief complaint. The detection rate and response amplitude of each DPOAE frequency, incubation period, and interval of ABR waves and -SP/AP in ECochG were analyzed. Results:①The thresholds of PTA at 0.25-8 kHz in both ears were in the normal range (P>0.05), and the thresholds of PTA at 9-16 kHz in affected ears were higher than those in healthy ears (P<0.001); ②There was statistical significance in the detection rate and response amplitude of DPOAE at 3, 4, 6, 8 kHz between ears (P<0.05); ③The incubation period of ABR Ⅰ wave in affected ears was (1.55±0.17) ms, that in the healthy ear was (1.50±0.14) ms, among them, the incubation period of ABR Ⅰ wave in the affected ear was longer than that in the healthy ear, and the difference was statistically significant (P<0.05); ④In ECochG, there was no significant difference in -SP amplitude between ears (P>0.05), but there was a significant difference in AP amplitude and -SP/AP amplitude between ears (P<0.05). Conclusion:EHFA, DPOAE, ABR, and ECochG have clinical significance in evaluating cochlear function in tinnitus patients.


Assuntos
Zumbido , Audiometria de Tons Puros , Cóclea , Potenciais Evocados Auditivos do Tronco Encefálico/fisiologia , Humanos , Emissões Otoacústicas Espontâneas/fisiologia , Zumbido/diagnóstico
20.
Bioorg Med Chem Lett ; 67: 128703, 2022 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-35364239

RESUMO

It is generally believed that EGFR/HER2 dual-target inhibitors may overcome the resistance of EGFR TKIs caused by HER2 overexpression. The structure-based synthesis and biological evaluation of quinazoline derivatives as EGFR/HER2 dual-target inhibitors has been studied in this paper. II-1, II-2, III-3, III-4 displayed comparable inhibitory potency against EGFR and HER2 and II-1 showed remarkable antiproliferative activities against NCI-H358/PC-9/Calu-3/NCI-H1781 (EGFR IC50 = 0.30 nM, HER2 IC50 = 6.07 nM, NCI-H358 GI50 = 23.30 nM, PC-9 GI50 = 1.95 nM, Calu-3 GI50 = 23.13 nM NCI-H1781 GI50 = 41.61 nM).


Assuntos
Antineoplásicos , Quinazolinas , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células , Ensaios de Seleção de Medicamentos Antitumorais , Receptores ErbB/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Quinazolinas/farmacologia , Relação Estrutura-Atividade
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