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1.
Medicine (Baltimore) ; 102(3): e32548, 2023 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-36701711

RESUMO

Laryngeal cancer (LC) is a malignant tumor that occurs in the head and neck. Laryngeal cancer is one of the most common cancers of the neck and head, and its prognosis has always been poor. The incidence of LC increased gradually and showed an early rising trend. Laryngeal cancer is rarely studied in relation to immunity, Malignant tumors will change the state of the human body in various ways to adapt to their own survival and avoid the immune system. This study aims to explore the immune molecular mechanism of laryngeal cancer through bioinformatics analysis. The gene expression data was downloaded for 3 microarray datasets: GSE27020, GSE59102, and GSE51985. CIBERSORT algorithm was performed to evaluate immune cell infiltration in tissues between LC and healthy control (HC). Differentially expressed genes (DEGs) were screened. Functional correlation of DEGs were analyzed by Gene Ontology, Gene Set Enrichment Analysis and Kyoto encyclopedia of genes and genomes. Candidate biomarkers were identified by cytoHubba of Cytoscape. Spearman correlations between the above biomarkers and infiltrating immune cells were explored using R software analysis. The immune cell types of LC and HC were significantly different. Twenty-one DEGs were obtained by cross-screening. The function of DEGs is closely related to the number of immune cells. Five central genes (TNNT3, TNNI2, Desmin, matrix metallopeptidase 9 and cytotoxic T lymphocyte antigen 4) were screened. The HUB gene was demonstrated to have the ability to diagnose LC and HC with good specificity and sensitivity. The correlation between immune cells and biomarkers showed that hub gene was positively correlated with macrophages and dendritic cells, and negatively correlated with CD4 + T cell. TNNT3, TNNI2, Desmin, matrix metallopeptidase 9 and cytotoxic T lymphocyte antigen 4 can be used as diagnostic biomarker for LC. Macrophages, dendritic cells and CD4 + T cell may participate in the occurrence and development of LC.


Assuntos
Carcinoma , Neoplasias Laríngeas , Humanos , Neoplasias Laríngeas/diagnóstico , Neoplasias Laríngeas/genética , Antígeno CTLA-4 , Desmina , Biologia Computacional , Endopeptidases , Metaloproteases , Biomarcadores , Perfilação da Expressão Gênica , Biomarcadores Tumorais/genética
2.
Math Biosci Eng ; 20(1): 1460-1487, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36650819

RESUMO

In order to cope with the rapid growth of flights and limited crew members, the rational allocation of crew members is a strategy to greatly alleviate scarcity. However, if there is no appropriate allocation plan, some flights may be canceled because there is no pilot in the scheduling period. In this paper, we solved an airline crew rostering problem (CRP). We model the CRP as an integer programming model with multiple constraints and objectives. In this model, the schedule of pilots takes into account qualification restrictions and language restrictions, while maximizing the fairness and satisfaction of pilots. We propose the design of two hybrid metaheuristic algorithms based on a genetic algorithm, variable neighborhood search algorithm and the Aquila optimizer to face the trade-off between fairness and crew satisfaction. The simulation results show that our approach preserves the fairness of the system and maximizes the fairness at the cost of crew satisfaction.


Assuntos
Algoritmos , Resolução de Problemas , Simulação por Computador
3.
J Pain Res ; 15: 3711-3728, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36484061

RESUMO

Introduction: With the wide acceptance of acupuncture, many papers and guidelines recommend that acupuncture is effective for chronic pain (CP). In this study, we applied bibliometric methods to analyze the current research situation of acupuncture intervention in CP, to gain insight into the current situation and future development trend of this field. Material and Methods: Science Citation Index Expanded was searched for publications related to acupuncture for CP between 1900 and 2022. VOSviewer, CiteSpace and Thomson Data Analyzer were used to analyze the annual publication, authors and cited authors and their countries (regions) and institutions, journals and cited journals, cited references, co-occurrence keywords, burst keywords, and the relevant centrality. Results: A total of 1968 papers were retrieved, the annual publications have shown a rapid growth trend in the recent 20 years. The USA (708) and the Kyung Hee University (31) were the most productive country and institution, respectively, while the USA (0.37) and University of Maryland (0.13) had the highest centrality. MacPherson, Hugh published the most papers in this field (29), and Vickers, A J were the most influential author (289 times cited). Journal of Alternative and Complementary Medicine was the most productive journal (92), whereas PAIN was the most influential one (4743 times cited). Breivik, H's (2006) paper had the most citation count (3025), while Furlan's (2005) paper had the highest centrality (0.23). The research focuses in this field mainly include pain, electroacupuncture, Low back pain, Systematic review, Randomized controlled trial etc. Researchers are currently paying more attention to the psychological problems caused by CP. Conclusion: The research of acupuncture for CP will be further expanded. International cooperation of this research field needs to be further strengthened. More high-quality designed trials need to be conducted.

4.
Sci Rep ; 12(1): 18896, 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36344573

RESUMO

People more accurately remember faces of their own racial group compared to faces of other racial groups; this phenomenon is called the other-race effect. To date, numerous researchers have devoted themselves to exploring the reasons for this other-race effect, and they have posited several theoretical explanations. One integrated explanation is the categorization-individuation model, which addresses two primary ways (categorization and individuation) of racial face processing and emphasizes the emergence of these two ways during the encoding stage. Learning-recognition and racial categorization tasks are two classical tasks used to explore racial face processing. Event-related potentials can facilitate investigation of the encoding differences of own- and other-race faces under these two typical task demands. Unfortunately, to date, results have been mixed. In the current study, we investigated whether categorization and individuation differ for own- and other-race faces during the encoding stage by using racial categorization and learning-recognition tasks. We found that task demands not only influence the encoding of racial faces, but also have a more profound effect in the encoding stage of recognition tasks for other-race faces. More specifically, own-race faces demonstrate deeper structural encoding than other-race faces, with less attentional involvement. Moreover, recognitions tasks might ask for more individual-level encoding, requiring more attentional resources in the early stage that may be maintained until relatively late stages. Our results provide some evidence concerning task selection for future racial face studies and establish a groundwork for a unified interpretation of racial face encoding.


Assuntos
Reconhecimento Facial , Humanos , Reconhecimento Psicológico , Potenciais Evocados , Grupos Raciais , Reconhecimento Visual de Modelos
5.
Front Public Health ; 10: 1000403, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36311639

RESUMO

The relationship between lead exposure and neurological disorders has been extensively studied, but the effects of lead exposure on hepatotoxicity are unknown. Metabolically related fatty liver disease (MAFLD) is an update of previous non-alcoholic fatty liver disease (NAFLD). It redefines the diagnostic conditions and emphasizes metabolic factors while considering non-alcoholic factors. Lead can affect the endocrine system and metabolism, so we believe that lead exposure may contribute to MAFLD. 41,723 individuals who had undergone blood lead testing from 2005 to 2018 in the National Health and Nutrition Examination Survey (NHANES) database were selected for this study. The characteristics of population lead exposure in the last decade or so, the effect of lead exposure on liver function and whether lead exposure can cause MAFLD were analyzed. Co-variates were adjusted according to age, ethnicity, body mass index (BMI), waist circumference, visceral adiposity index (VAI), poverty indices (PIR), diabetes, hypertension, and hyperlipidemia. The results showed that blood lead concentrations stabilized at a low level after a decreasing trend from year to year. The differences in blood lead concentrations were associated with differences in age, sex, race, education level, and PIR. Lead exposure was an independent risk factor for MAFLD, and lead and nine other factors were used as independent risk factors for MAFLD, so a nomogram was established to predict the prevalence probability of MAFLD.


Assuntos
Chumbo , Hepatopatia Gordurosa não Alcoólica , Humanos , Prevalência , Chumbo/efeitos adversos , Inquéritos Nutricionais , Nomogramas , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Hepatopatia Gordurosa não Alcoólica/etiologia
6.
Ann Clin Lab Sci ; 52(5): 850-857, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36261174

RESUMO

The World Health Organization in its 2019 Classification of Breast Tumors termed breast sebaceous carcinoma as invasive breast carcinoma of no special type (IBC-NST), with a sebaceous pattern. Approximately 30 cases of IBC-NST with a sebaceous pattern have been reported in the literature, and in all cases the expression of mismatch repair proteins in tumors was normal. Here, we report a case of IBC-NST with a sebaceous pattern and high-frequency microsatellite instability (MSI-H). This case was a sporadic sebaceous pattern of IBC-NST with MSI-H and was unrelated to Muir-Torre syndrome. Its histopathological characteristics were similar to those of MSI-H-associated triple-negative breast carcinoma (TNBC) with a high histological grade but were without tumor-infiltrating lymphocytes (TILs). The tumor did not recur after 20 months of follow-up.


Assuntos
Neoplasias da Mama , Síndrome de Muir-Torre , Humanos , Feminino , Instabilidade de Microssatélites , Recidiva Local de Neoplasia , Síndrome de Muir-Torre/metabolismo , Síndrome de Muir-Torre/patologia , Neoplasias da Mama/genética , Linfócitos do Interstício Tumoral/metabolismo
7.
Nat Commun ; 13(1): 6144, 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36253372

RESUMO

The greatest challenge that limits the application of pyro-catalytic materials is the lack of highly frequent thermal cycling due to the enormous heat capacity of ambient environment, resulting in low pyro-catalytic efficiency. Here, we introduce localized plasmonic heat sources to rapidly yet efficiently heat up pyro-catalytic material itself without wasting energy to raise the surrounding temperature, triggering a significantly expedited pyro-catalytic reaction and enabling multiple pyro-catalytic cycling per unit time. In our work, plasmonic metal/pyro-catalyst composite is fabricated by in situ grown gold nanoparticles on three-dimensional structured coral-like BaTiO3 nanoparticles, which achieves a high hydrogen production rate of 133.1 ± 4.4 µmol·g-1·h-1 under pulsed laser irradiation. We also use theoretical analysis to study the effect of plasmonic local heating on pyro-catalysis. The synergy between plasmonic local heating and pyro-catalysis will bring new opportunities in pyro-catalysis for pollutant treatment, clean energy production, and biological applications.

8.
Medicine (Baltimore) ; 101(40): e30888, 2022 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-36221371

RESUMO

Esophageal cancer (ESCA), one of the most aggressive malignant tumors, has been announced to be the ninth most common cancer and the sixth leading cause of cancer-related death in the world. Chromobox family members (CBXs) are important epigenetic regulators which are related with the transcription of target genes. The role of CBXs in carcinomas has been reported in many studies. However, the function and prognostic value of different CBXs in ESCA are still largely unknown. In this article, we first performed differential expression analysis through several methods including Oncomine and Gene Expression Profiling Interactive Analysis. The results led us to determine the differential expression of CBXs in pan-cancer, especially ESCA. Then we evaluated the prognostic value of different CBX messenger RNA (mRNA) expression in patients with ESCA through the Kaplan-Meier plotter and the Human Protein Atlas database. In addition, we used cBioPortal to explore all genetic alterations and mutations in the CBXs in ESCA. Simultaneously, the correlation between its expression and the level of immune infiltration of ESCA was visualized by TIMER. Finally, the biological function of CBXs in ESCA is obtained through Biological Enrichment Analysis including gene ontology and Kyoto Encyclopedia of Genes and Genomes. The expression levels of CBX3/4/5 and CBX8 in ESCA tissues increased significantly and the expression level of CBX7 decreased through differential expression analysis. Additionally, CBX1 is significantly related to the clinical cancer stage and disease-free survival of ESCA patients. The high mRNA expression of CBX4 is related to the short overall survival of patients with esophageal squamous cell carcinoma, and the high mRNA expression of CBX3/7/8 is related to the short overall survival of patients with esophageal adenocarcinoma, indicating that CBX1/3/4/7/8 may be a potential prognostic biomarker for the survival of ESCA patients. Besides, the expression of CBXs is significantly related to the infiltration of a variety of immune cells, including six types of CD4-positive T-lymphocytes, macrophages, neutrophils, bursindependentlymphocyte, CD8-positive T-lymphocytes cells and dendritic cells in ESCA. Moreover, we found that CBXs are mainly associated with the inhibition of cell cycle and apoptosis pathway. Further, enrichment analysis indicated that CBXs and correlated genes were enriched in mismatch repair, DNA replication, cancer pathways, and spliceosomes. Our research may provide new insights into the choice of prognosis biomarkers of the CBXs in ESCA.


Assuntos
Neoplasias Esofágicas , Carcinoma de Células Escamosas do Esôfago , Biomarcadores , Proteínas Cromossômicas não Histona , Neoplasias Esofágicas/genética , Humanos , Ligases , Complexo Repressor Polycomb 1 , Proteínas do Grupo Polycomb/genética , Proteínas do Grupo Polycomb/metabolismo , Prognóstico , RNA Mensageiro/metabolismo
9.
Biomed Res Int ; 2022: 4501186, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36193326

RESUMO

Background: Osteonecrosis of the femoral head (ONFH) is a disabling orthopedic disease, which is impacted by infiltration of immune cells. Thus, the aim of the current research was to determine the inflammation-related biomarkers in ONFH. Methods: GSE123568 dataset with control and steroid-induced osteonecrosis of the femoral head (SONFH) samples were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were detected by limma R package and weighted gene co-expression network analysis (WGCNA) was used to explore the co-expression genes and modules. We obtained inflammation-related genes (IRGs) from the Molecular Signatures Database (MSigDB). Then, the IRGs associated with SONFH (IRGs-SONFH) were screened out and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. A protein-protein interaction (PPI) network was established using the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database, and hub genes were identified by the MCODE algorithm. Based on the hub genes, we constructed a lncRNA-miRNA-mRNA network. Results: We identified 535 DEGs between control and SONFH samples. The WGCNA clearly indicated that the brown module was most significantly associated with SONFH. We identified 25 IRGs-SONFH through WGCNA module genes, DEGs and IRGs. A total of 4 hub genes (CD14, CYBB, NOD2, and TLR1) were identified by Cytoscape. Receiver operating characteristic (ROC) curve analysis determined that the expressions of the four genes could distinguish SONFH from controls as evidenced by the area under the curve (AUC) greater than 0.7. Finally, we constructed a competitive endogenous RNA (ceRNA) network which included 67 lncRNAs, 1 miRNA (hsa-miR-320a), and 1 mRNA (NOD2). Conclusions: Our study identified 4 hub genes as potential inflammation-related biomarkers of SONFH. Moreover, we proposed a ceRNA network of lncRNAs targeting hsa-miR-320a, hsa-miR-320a, and NOD2 as a potential RNA regulatory pathway that controls disease progression in ONFH.


Assuntos
MicroRNAs , Osteonecrose , RNA Longo não Codificante , Biomarcadores/metabolismo , Cabeça do Fêmur/metabolismo , Redes Reguladoras de Genes , Humanos , Inflamação/genética , Inflamação/metabolismo , MicroRNAs/genética , Osteonecrose/induzido quimicamente , RNA Longo não Codificante/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Esteroides/efeitos adversos , Receptor 1 Toll-Like/genética , Receptor 1 Toll-Like/metabolismo
10.
J Phys Chem A ; 126(36): 6124-6134, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-36069475

RESUMO

Sensor X is a turn-on sensor, which is applied in the fluorescence detection of Zn2+ ions. Its photophysical process is comprehensively investigated to clarify its weak fluorescence. With the aid of density functional theory (DFT) and time-dependent density functional theory (TDDFT), the potential energy surfaces (PES) of X on both ground and first excited states are studied. Excited-state intramolecular proton transfer (EPT) processes as well as molecule twisting motion are observed, which induces several minima on the excited-state PES. Transition states as well as rate constants for these dynamic processes are obtained to evaluate their occurrences. The twisting motion of the sensor is an ultrafast process, which is initiated by a specific EPT process and leads to a nonemissive twisted intramolecular charge transfer (TICT) state. The fluorescence of the sensor is barely observable because of the easily attainable TICT state on the excited PES. This mechanism is trustworthy and intrinsically different from the previously proposed mechanism. After clarifying the photophysical process of the sensor, the Zn2+ sensing mechanism is uncovered. Also, the selectivity against Cd2+ and Hg2+ is fully discussed.

11.
J Biochem Mol Toxicol ; 36(10): e23156, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36156333

RESUMO

Breast cancer is becoming a common life-threatening disease, especially in women, along with higher incidence and mortality. MicroRNA (miR)-506 was reported to participate in breast cancer progression, while the role of miR-506 in breast cancer-induced osteolytic bone metastasis is unclear. In the present study, we found significant downregulation of miR-506 in breast cancer tissues and cell lines. Overexpression of miR-506 notably reduced the proliferative, migratory and invasive rates of MCF7 and MDA-MB-231 cells, and reduced the production of inflammatory factors IL-6 and TNF-α in MCF7 cells. Moreover, overexpression of miR-506 obviously inhibited tumor growth in an in vivo animal model. In addition, overexpression of miR-560 efficiently attenuated breast cancer-induced osteolysis in vivo, which was characterized by increased bone volume/total volume (BT/TV), trabecular number (Tb. N), and trabecular thickness (Tb. Th), as well as the reduced trabecular separation (Tb. Sp). The nuclear factor of activated T cell cytoplasmic 1 (NFATc1) was identified as a downstream target of miR-506, and overexpression of miR-506 could inhibit breast cancer progression by targeting NFATc1. Furthermore, our results showed that NFATc-1 might participate in the inhibition of miR-506 on breast cancer-induced osteolysis. In conclusion, our findings provide insights into understanding the pathogenesis of breast cancer and breast cancer-induced osteolytic bone metastasis, and miR-506 might serve as a novel biomarker for this disease.


Assuntos
Neoplasias Ósseas , MicroRNAs , Osteólise , Animais , Neoplasias Ósseas/metabolismo , Linhagem Celular Tumoral , Feminino , Interleucina-6 , MicroRNAs/genética , MicroRNAs/metabolismo , Osteólise/etiologia , Osteólise/metabolismo , Osteólise/patologia , Transdução de Sinais , Fatores de Transcrição , Fator de Necrose Tumoral alfa
12.
Neuropsychiatr Dis Treat ; 18: 1861-1873, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36052274

RESUMO

Purpose: PSD is a syndrome that occurs after a stroke, which manifests as a series of depressive symptoms and corresponding physiological symptoms. Relevant studies have shown that the drug therapy is often accompanied by drug side effects and patient resistance. Acupuncture has attracted attention as a treatment method without adverse reactions of patients. The purpose of this study was to investigate the possible mechanism of action of acupuncture in PSD. Patients and Methods: Download depression and stroke datasets from public databases. Bioinformatics methods were used to analyze the key gene targets related to stroke and depression. Functional enrichment analysis assesses important pathways. Further screen PSD-related biological pathways and genes. After the experimental model was established, the expression differences of key genes and related pathways were compared between the model group and the control group through acupuncture treatment and qPCR verification. Results: Depression and stroke-related genes were obtained by bioinformatics methods, and then important biological processes and biological pathways related to depression and stroke were analyzed by GO and KEGG. And further screen out the disease targets closely related to PSD. In the follow-up animal experiments, we confirmed that acupuncture can intervene on these key pathways and targets, and then play a role in the targeted therapy of diseases. Conclusion: The results of this study show that five genes ("NRBP1", "SIRT1", "BDNF", "MAPK3", "CREB1".) and key biological pathways such as NFkB, PI3K/AKT activation, and MAPK are the keys to the occurrence and development of PSD biomarkers, which can also be therapeutically intervened by acupuncture.

13.
Carbohydr Polym ; 295: 119881, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-35988986

RESUMO

Cyanobacterial biomass and cellulose-based materials have been used separately as green bio-adsorbents for the removal of toxic metals from water. Hybrid materials made of living microbial cells encased in a solid matrix have shown good potential for bioremediation. Here, the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 was embedded in situ into bacterial cellulose (BC), a robust biopolymer rich in hydroxyl groups with excellent water holding capacity. The living material was obtained by injecting S. elongatus into a Komagataeibacter sucrofermentans culture producing BC. Several types of BC/S. elongatus (BC/SE) materials were developed including small spheroids and flat films with different cyanobacteria loads via simple adjustments of the biosynthesis process parameters. BC/SE spheroids were evaluated for toxic copper removal and exhibited excellent adsorption properties compared to pure BC with a maximum capacity of 156.25 mg g-1. Thus, this simple bio-embedding approach holds promises in the development of living materials for environmental applications.


Assuntos
Celulose , Água , Adsorção , Biodegradação Ambiental , Biomassa
14.
Bioresour Technol ; 361: 127711, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35907600

RESUMO

Gas fermentation is a well-established process for the conversion of greenhouse gases from industrial wastes into valuable multi-carbon chemicals. Here, a two-stage process was developed to expand the product range of gas fermentation and synthesized the versatile biopolymer bacterial cellulose (BC). In the first stage, the acetogen Clostridium autoethanogenum was cultivated with H2:CO:CO2 and produced ethanol and acetate. In the second stage, BC-synthesizing Komagataeibacter sucrofermentans was grown in the spent medium from gas fermentation. K. sucrofermentans was able to produce BC autotrophically from gas-derived metabolites alone as well as mixotrophically with the addition of exogenous glucose. In these circumstances, 1.31 g/L BC was synthesized with a major energetic contribution from C1 gas fermentation products. Mixotrophic BC characterization reveals unique properties including augmented mechanical strength, porosity, and crystallinity. This proof-of-concept process demonstrates that BC can be produced from gases and holds good potential for the efficient conversion of C1 wastes.


Assuntos
Celulose , Gases , Processos Autotróficos , Ciclo do Carbono , Celulose/química , Fermentação , Gases/metabolismo
15.
Front Endocrinol (Lausanne) ; 13: 897439, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35784575

RESUMO

Introduction: Osteonecrosis of the femoral head (ONFH) is a progressive and disabling disease with severe socioeconomic burdens. In the last 30 years, a growing number of publications have reported significant advances in understanding ONFH. However, only a few studies have clarified its global trends and current status. Thus, the purpose of our study was to summarize the global trends and current status in ONFH through bibliometrics. Materials and Methods: Publications related to ONFH from 1991 to 2020 were searched from the Web of Science (WOS) core collection database. The data were analyzed with bibliometric methods. Microsoft Excel was used for statistical analysis and to draw bar charts. SPSS was applied to perform linear regression analysis. VOSviewer was used to conduct bibliographic coupling analysis, co-authorship analysis, co-citation analysis and co-occurrence analysis. Results: A total of 5,523 publications were covered. The United States consistently ranked first in total publications, sum of times cited, average citations per item and H-index. Kyushu University was the main contributor to ONFH. Clinical Orthopaedics and Related Research was the major publishing channels for ONFH-related articles. Takuaki Yamamoto published the most ONFH-related articles. Studies regarding ONFH could be divided into five clusters: 1) mechanism study, 2) treatment study, 3) complication study, 4) radiological study and 5) etiological study. Mechanism study might become a hot spot in the future. Conclusions: The total number of publications in ONFH has generally increased over the last three decades. The United States was the leading country in ONFH research. Transplantation, engineering, cell and molecular biology, pharmacology and endocrinology have gradually increased and become hot topics in ONFH research. Mechanism study in ONFH including mesenchymal stem cells, apoptosis, oxidative stress, adipogenesis, osteogenic differentiation and endothelial progenitor cells, have attracted more attention and will become a hot spot in the future.


Assuntos
Cabeça do Fêmur , Osteonecrose , Bibliometria , Humanos , Osteogênese , Publicações , Estados Unidos
16.
J Colloid Interface Sci ; 626: 89-100, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-35780555

RESUMO

The existence of sulfur dioxide and water vapor in the flue gas generated from waste-to-energy stations could lead to catalyst deactivation, which has adverse effects on NOx removal. It is thus particularly important to study the reaction mechanism of catalyst resistance to poisoning. Herein, we report the mechanism of In-Co3O4-Ga2O3/H-Beta catalyst to SO2 and H2O resistance in the selective catalytic reduction (SCR) of NOx by CH4. The catalyst could achieve 74.6% NOx removal efficiency in the presence of 100 ppm SO2 and 5% H2O. In this catalyst, Co3O4 is attributed to enhancing the reversible poisoning of SO2 and CH4 activation and increasing the number of Brønsted acid sites by decomposing H2O. However, the InO+ active center was still eroded by a small amount of water vapor, leading to a reduction in NOx removal efficiency. The addition of Ga2O3 primarily provided an important intermediate NO2 for CH4-SCR reaction and reduced the aggregates of Co3O4 to increase the exposure of indium sites, and could reduce a part of SO2 to S2-. This study provides a good candidate for preparing catalysts with superior resistance towards SO2 and H2O for CH4-SCR.


Assuntos
Amônia , Vapor , Catálise , Cobalto , Oxirredução , Óxidos
17.
Oxid Med Cell Longev ; 2022: 5905374, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35770043

RESUMO

Atrial natriuretic peptide (ANP), a canonical cardiac hormone, is mainly secreted from atrial myocytes and is involved in the regulation of body fluid, blood pressure homeostasis, and antioxidants. Cholecystokinin (CCK) is also found in cardiomyocytes as a novel cardiac hormone and induces multiple cardiovascular regulations. However, the direct role of CCK on the atrial mechanical dynamics and ANP secretion is unclear. The current study was to investigate the effect of CCK octapeptide (CCK-8) on the regulation of atrial dynamics and ANP secretion. Experiments were performed in isolated perfused beating rat atria. ANP was measured using radioimmunoassay. The levels of hydrogen peroxide (H2O2) and arachidonic acid (AA) were determined using ELISA Kits. The levels of relative proteins and mRNA were detected by Western blot and RT-qPCR. The results showed that sulfated CCK-8 (CCK-8s) rather than desulfated CCK-8 increased the levels of phosphorylated cytosolic phospholipase A2 and AA release through activation of CCK receptors. This led to the upregulation of NADPH oxidase 4 (NOX4) expression levels and H2O2 production and played a negative inotropic effect on atrial mechanical dynamics via activation of ATP-sensitive potassium channels and large-conductance calcium-activated potassium channels. In addition, CCK-8s-induced NOX4 subsequently upregulated peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α) expression levels through activation of p38 mitogen-activated protein kinase as well as the serine/threonine kinase signaling, ultimately promoting the secretion of ANP via activation of PPARα and PPARγ. In the presence of the ANP receptor inhibitor, the CCK-8-induced increase of AA release, H2O2 production, and the upregulation of NOX4 and CAT expressions was augmented but the SOD expression induced by CCK-8s was repealed. These findings indicate that CCK-8s promotes the secretion of ANP through activation of NOX4-PGC-1α-PPARα/PPARγ signaling, in which ANP is involved in resistance for NOX4 expression and ROS production and regulation of SOD expression.


Assuntos
Fator Natriurético Atrial , PPAR gama , Animais , Fator Natriurético Atrial/metabolismo , Átrios do Coração/metabolismo , Peróxido de Hidrogênio/farmacologia , NADPH Oxidase 4/metabolismo , PPAR alfa/metabolismo , PPAR gama/metabolismo , Ratos , Sincalida/metabolismo , Sincalida/farmacologia , Superóxido Dismutase/metabolismo
18.
J Vis Exp ; (182)2022 04 12.
Artigo em Inglês | MEDLINE | ID: mdl-35499357

RESUMO

Osteonecrosis of the femoral head (ONFH) is a common joint disease in young and middle-aged patients, which seriously burdens their lives and work. For early-stage ONFH, core decompression surgery is a classical and effective hip preservation therapy. In traditional procedures of core decompression with Kirschner wire, there are still many problems such as X-ray exposure, repeated puncture verification, and damage to normal bone tissue. The blindness of the puncture process and the inability to provide real-time visualization are crucial reasons for these problems. To optimize this procedure, our team developed an intraoperative navigation system on the basis of augmented reality (AR) technology. This surgical system can intuitively display the anatomy of the surgical areas and render preoperative images and virtual needles to intraoperative video in real-time. With the guide of the navigation system, surgeons can accurately insert Kirschner wires into the targeted lesion area and minimize the collateral damage. We conducted 10 cases of core decompression surgery with this system. The efficiency of positioning and fluoroscopy is greatly improved compared to the traditional procedures, and the accuracy of puncture is also guaranteed.


Assuntos
Realidade Aumentada , Necrose da Cabeça do Fêmur , Procedimentos Ortopédicos , Descompressão , Cabeça do Fêmur/cirurgia , Necrose da Cabeça do Fêmur/diagnóstico por imagem , Necrose da Cabeça do Fêmur/cirurgia , Humanos , Pessoa de Meia-Idade
20.
Curr Rheumatol Rev ; 18(3): 178-185, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35366779

RESUMO

Osteoarthritis (OA) is a whole joint disease that is significantly related to abnormal mechanical loads. Subchondral bone alterations, during the evolution course of OA, are considered a reflection of the adaptation of the bone tissue to mechanical loads. However, some of these alterations are taken as a detriment and paradoxical. What are these structure, composition, and mechanical property alterations or mechanical functions for are not quite clear. In this review, we discuss the possibility that these alterations are used for maintaining the joint function. With taking excessive load as a risk factor and under conditions of articular cartilage gradually lose its thickness and its function of evenly distributing the load on the subchondral bone plate, and applying Poroelasticity to bone mechanics; moreover, Boussinesq's pressure bulb theory and bone optimal design principles are utilized. We found that each subchondral bone alteration has its unique mechanical function in resisting loads and maintaining the joint function, and these alterations comply with both bone optimal design principles and Wolff's law within a proper range.


Assuntos
Cartilagem Articular , Osteoartrite , Osso e Ossos , Humanos
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