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1.
Artigo em Inglês | MEDLINE | ID: mdl-31851092

RESUMO

OBJECTIVE: Radiofrequency ablation (RFA) is an effective and minimally invasive treatment for managing hepatic hemangiomas. Systemic inflammatory response syndrome (SIRS) often occurs with hemoglobinuria, and its underlying pathophysiological mechanism is unknown. Heme can trigger inflammation by inducing the generation of reactive oxygen species (ROS) and the production of inflammatory mediators. We therefore investigated whether circulating heme is involved in SIRS following RFA of hepatic hemangiomas. METHODS: We enrolled 65 patients with hepatic hemangioma who underwent RFA. Serum concentrations of free heme, ROS, and tumor necrosis factor α (TNF-α) were measured after RFA. Univariate analysis and a multivariate binary logistic regression model were used to evaluate the contribution of 17 risk factors for SIRS after RFA. RESULTS: Fifty-nine (59/65, 90.8%) patients developed hemoglobinuria, among which 25 (25/59, 42.4%) experienced SIRS shortly after RFA. In the SIRS group, the serum concentrations of heme, ROS, and TNF-α were immediately elevated after RFA compared with baseline and slowly regained their normal levels 3 days after RFA. Moreover, the concentrations of circulating heme significantly correlated with those of ROS (r = 0.805, P < 0.001) and TNF-α (r = 0.797, P < 0.001). Multivariate analysis showed that the volume of hemangioma [odds ratio (OR) = 1.293, P = 0.031], time of ablation (OR = 1.194, P = 0.029) as well as the concentrations of heme (OR = 1.430, P = 0.017), ROS (OR = 1.251, P = 0.031), and TNF-α (OR = 1.309, P = 0.032) were significantly associated with SIRS. CONCLUSION: Circulating heme was associated with the induction of ROS and the production of TNF-α, which may contribute to the induction of SIRS following RFA of hepatic hemangiomas.

2.
Onco Targets Ther ; 12: 6839-6842, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31692527

RESUMO

Hepatic sclerosing hemangioma is a rare benign disease that occurs in association with hepatic cavernous hemangioma degeneration and sclerosis. Recent studies have shown that radiofrequency (RF) ablation is an alternative treatment for hepatic cavernous hemangiomas, even for large hemangiomas (≥10 cm). However, RF ablation might not be suitable to treat large sclerosing hemangiomas. We herein report the successful surgical removal of a large hepatic sclerosing hemangioma after RF ablation treatment failure in a 65-year-old man. In conclusion, we suggest that resection should be chosen as a first-line therapy for the disease.

3.
Anim Nutr ; 5(3): 307-313, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31528734

RESUMO

The objective of this paper was to evaluate the effect of vanadium (V) in high-fat diets sourced from egg yolk on body weight gain, feed intake, blood characteristics and antioxidative status of Wistar rats. A total of 72 female Wistar rats were allocated according to a 2 × 4 factorial design throughout a 5-wk trial, including 2 levels of dietary fat (normal and high; ether extract 40.3 and 301.2 g/kg; fat sourced from egg yolk) and 4 levels of dietary V (0, 3, 15 and 30 mg/kg). Vanadium decreased (P ≤ 0.05) body weight gain (V at 30mg/kg during wk 1 and 2; V at 15 and 30 mg/kg during the overall phase), feed intake (V at 30 mg/kg during wk 3 and the overall phase; V at 15 and 30 mg/kg during wk 4), but increased the relative weight of liver (V at 30 mg/kg, P ≤ 0.05). Moreover, increasing dietary V significantly increased (P ≤ 0.05) plasma aspartate aminotransferase, alanine aminotransferase and malondialdehyde levels and decreased triglyceride level, and V at 30 mg/kg in high-fat treatment had the highest or lowest values (interaction, P ≤ 0.05). Under the same dietary V dose, V residual content in liver (dietary V at 15 and 30 mg/kg) and kidney (dietary V at 15 mg/kg) was higher in high-fat diet treatment compared with normal-fat diet treatment (P ≤ 0.05). In conclusion, it is suggested that V could decrease the body weight together with the feed intake, and the high fat could enhance oxidative stress induced by V of Wistar rats.

4.
Open Med (Wars) ; 14: 398-402, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31157306

RESUMO

In recent years, radiofrequency (RF) ablation has been increasingly used for treating hepatic hemangiomas attributing to its unique advantages, such as minimal invasiveness, definite efficacy, high safety, fast recovery, and wide applicability. However, complications related to RF ablation had been frequently reported, especially while being used for treating huge hemangioma (≥10 cm). Cautious measures had been taken to prevent the incidence of ablation-induced complications, but still unexpected complications occurred. Herein we reported a case of severe myocardial dysfunction along with systemic inflammatory response syndrome occurring immediately post RF ablation of a 10.7 cm hemangioma. This serious complication was effectively managed by supportive care with the full recovery in a short period of time.

5.
BMB Rep ; 52(4): 277-282, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30940322

RESUMO

Currently speaking, it is noted that radiofrequency ablation (RFA) has been the most widely used treatment for hepatocellular carcinoma (HCC) occurring in patients. However, accumulating evidence has demonstrated that the incidence of insufficient RFA (IRFA) may result in the identified rapid progression of residual HCC in the patient, which can greatly hinder the effectiveness and patient reported benefits of utilizing this technique. Although many efforts have been proposed, the underlying mechanisms triggering the rapid progression of residual HCC after IRFA have not yet been fully clarified through current research literature reviews. It was shown in this study that cell proliferation, migration and invasion of residual HepG2 and SMMC7721 cells were significantly increased after the IRFA was simulated in vitro. In other words, it is noted that IRFA could do this by enhancing the image of autophagy of the residual HCC cell via the HIF-1α/BNIP3 pathway. Consequently, the down-regulation of BNIP3 may result in the inhibition of the residual HCC cell progression and autophagy after IRFA. Our present study results suggest that IRFA could promote residual HCC cell progression in vitro by enhancing autophagy via the HIF-1α/BNIP3 pathway. For this reason, it is noted that the targeting of the BNIP3 may be useful in preventing the rapid growth and metastasis of residual HCC after IRFA. [BMB Reports 2019; 52(4): 277-282].


Assuntos
Carcinoma Hepatocelular/terapia , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Neoplasias Hepáticas/terapia , Proteínas de Membrana/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Ablação por Radiofrequência/métodos , Autofagia/efeitos da radiação , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Progressão da Doença , Células Hep G2 , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Ablação por Radiofrequência/tendências , Transdução de Sinais
6.
Environ Toxicol ; 33(12): 1312-1320, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30251772

RESUMO

Vanadium (V) can induce cell apoptosis in layers' oviduct resulting in egg quality reduction. In this study, we investigated the relationship between the mitogen-activated protein kinase (MAPK)-signaling pathway and V-induced apoptosis in poultry oviduct magnum epithelial cells (OMECs). Cultured OMECs were divided into 8 treatment groups: 0 µmol/L V (control), 100 µmol/L V (V100), V100 + P38MAPK inhibitor (SB203580), SB203580, V100 + extracellular signal-regulated kinases 1 and 2 (ERK1/2) inhibitor (U0126), U0126, V100 + c-JUN NH2 -terminal kinase (JNK) inhibitor (SP600125), and SP600125. The OMECs were pretreated with the MAPK inhibitors before their treatment with V100 for 12 h. V100 increased the apoptosis of OMECs (P < .05), while 3 MAPK inhibitors suppressed V100-induced apoptosis P < .05); V100 enhanced the depolarization of △ψm (P < .05), and SB203580 and U0126 alleviated the V100-induced △ψm decrease (P < .05); V100 downregulated B-cell lymphoma-2 (Bcl-2) and poly [Adenosine diphosphate ribose] polymerase 1 (PARP1) mRNA expression (P < .05), meanwhile it upregulated Bcl-2 associated x (Bax), Apaf1, cytochrome C (CytC) and cysteine aspartase (caspase) 3, 8, 9 mRNA expression (P < .05). All MAPKs inhibitors alleviated the up-regulation of V100 for Bax and caspase 3 mRNA expression and down-regulation of V100 for Bcl-2 expression (P < .05). SB203580 and U0126 upregulated CytC expression treated by V100 (P < .05), except SP600125, while SB203580 administration resulted in a similar upregulation of PARP1 expression (P < .05). SP600125 can alleviated V triggered p-P38MAPK (phosphor-P38), p-ERK1/2 (phosphor-ERK1/2), p-JNK (phosphor-JNK) increase on OME cells, and SB203580 and U0126 had a similar response to phosphor-P38 and p-JNK (P < .05). It concluded that V-induced apoptosis in OMECs through the activation of P38 and ERK1/2, and by increasing the ratio of Bax/Bcl-2, which resulted in △ψm decrease, CytC release into the cytosol; consequently caspase 3 is recruited and activated, PARP1 is cleaved, eventually leading to apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Células Epiteliais/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/fisiologia , Mitocôndrias/efeitos dos fármacos , Oviductos/efeitos dos fármacos , Vanádio/toxicidade , Proteínas Quinases p38 Ativadas por Mitógeno/fisiologia , Animais , Células Cultivadas , Galinhas , Células Epiteliais/fisiologia , Feminino , Mitocôndrias/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Oviductos/citologia
7.
World J Gastroenterol ; 23(39): 7077-7086, 2017 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-29093616

RESUMO

Recent studies have shown that radiofrequency (RF) ablation therapy is a safe, feasible, and effective procedure for hepatic hemangiomas, even huge hepatic hemangiomas. RF ablation has the following advantages in the treatment of hepatic hemangiomas: minimal invasiveness, definite efficacy, high safety, fast recovery, relatively simple operation, and wide applicability. It is necessary to formulate a widely accepted consensus among the experts in China who have extensive expertise and experience in the treatment of hepatic hemangiomas using RF ablation, which is important to standardize the application of RF ablation for the management of hepatic hemangiomas, regarding the selection of patients with suitable indications to receive RF ablation treatment, the technical details of the techniques, therapeutic effect evaluations, management of complications, etc. A final consensus by a Chinese panel of experts who have the expertise of using RF ablation to treat hepatic hemangiomas was reached by means of literature review, comprehensive discussion, and draft approval.


Assuntos
Ablação por Cateter , Hemangioma/cirurgia , Neoplasias Hepáticas/cirurgia , Ablação por Cateter/efeitos adversos , Ablação por Cateter/mortalidade , China , Consenso , Hemangioma/mortalidade , Hemangioma/patologia , Humanos , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/patologia , Complicações Pós-Operatórias/etiologia , Fatores de Risco , Resultado do Tratamento
8.
Medicine (Baltimore) ; 95(49): e5472, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27930527

RESUMO

This retrospective cohort study investigated the feasibility of radiofrequency (RF) ablation as an alternative to surgical intervention in patients with huge multiloculated pyogenic liver abscesses (PLAs).From August 2010 to April 2016, 83 patients with PLA were admitted to Beijing Chao-Yang Hospital, China. Four of these patients had huge multiloculated PLAs and underwent RF ablation plus antibiotics. The inclusion criteria for RF ablation were as follows: multiloculated PLA confirmed by computed tomography (CT) or magnetic resonance imaging (MRI), widest diameter of the PLA ≥5 cm, failure to respond to or not suitable to treatment with percutaneous drainage (PD), and patient refusal of surgery. The safety and effectiveness of RF ablation were initially assessed. All patients were commenced on antibiotics on admission to our hospital. CT-guided percutaneous catheter drainage was attempted in one patient but was unsuccessful. The main organism isolated from cultures of these patients' blood or abscess samples was Klebsiella pneumoniae (3/4). RF ablation was performed as soon as eligibility according to the above criteria was established.RF ablation was technically successful in all 4 study patients, all PLAs being completely eradicated. The median duration of fever after RF ablation was 4.5 days. No abscesses recurred; thus, this strategy for managing PLA was 100% successful (4/4). No procedure-related deaths or major complications occurred. One patient had an asymptomatic right pleural effusion that resolved with conservative treatment including albumin infusion and diuretics.Our preliminary data indicate that RF ablation is a safe, feasible, and effective treatment for huge multiloculated PLAs. It should be considered as an alternative treatment for patients who fail to respond to or not suitable for PD plus antibiotics and refuse surgical intervention.


Assuntos
Abscesso Hepático Piogênico/cirurgia , Adulto , Idoso , Antibacterianos/administração & dosagem , Ablação por Cateter , Estudos de Coortes , Infecções por Escherichia coli/diagnóstico por imagem , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/cirurgia , Estudos de Viabilidade , Feminino , Humanos , Infecções por Klebsiella/diagnóstico por imagem , Infecções por Klebsiella/tratamento farmacológico , Infecções por Klebsiella/cirurgia , Laparoscopia , Abscesso Hepático Piogênico/diagnóstico por imagem , Abscesso Hepático Piogênico/tratamento farmacológico , Imagem por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X , Resultado do Tratamento
9.
Rev Sci Instrum ; 87(8): 083108, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27587101

RESUMO

A fiber-coupled displacement measuring interferometer capable of determining of the posture of a reflective surface of a measuring mirror is proposed. The newly constructed instrument combines fiber-coupled displacement and angular measurement technologies. The proposed interferometer has advantages of both the fiber-coupled and the spatially beam-separated interferometer. A portable dual-position sensitive detector (PSD)-based unit within this proposed interferometer measures the parallelism of the two source beams to guide the fiber-coupling adjustment. The portable dual PSD-based unit measures not only the pitch and yaw of the retro-reflector but also measures the posture of the reflective surface. The experimental results of displacement calibration show that the deviations between the proposed interferometer and a reference one, Agilent 5530, at two different common beam directions are both less than ±35 nm, thus verifying the effectiveness of the beam parallelism measurement. The experimental results of angular calibration show that deviations of pitch and yaw with the auto-collimator (as a reference) are less than ±2 arc sec, thus proving the proposed interferometer's effectiveness for determination of the posture of a reflective surface.

10.
Appl Opt ; 55(15): 4073-8, 2016 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-27411134

RESUMO

A dual position-sensitive-detector (PSD)-based unit can be used for angular measurements of a multi-degree-of-freedom measurement system and a laser interferometry-based sensing and tracking system. In order to ensure the precision of incident beam direction measurement for a PSD-based unit, model and autoreflection alignment methods for correction of PSD-based unit parameters are proposed. Experimental results demonstrate the deviations between the angular measurements obtained using a dual PSD-based unit and an autocollimator varied by 70″, 20″, and 1″ for three runs of the autoreflection alignment method, respectively, and the model method deviations all varied by 1″ in the 1000″ measurement range for three runs. It is therefore concluded that the model method is more reliable than the autoreflection alignment method for ensuring the accuracy of a dual PSD-based unit.

11.
Mol Med Rep ; 13(6): 5029-2036, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27108693

RESUMO

MicroRNAs (miRNAs) are involved in cancer development and progression. Renal cell carcinoma (RCC) frequently undergoes metastasis and has a high mortality rate. The current study measured miRNA­126 (miR­126) expression levels in 128 pairs of clear cell RCC and adjacent normal kidney tissue samples by reverse transcription­quantitative polymerase chain reaction, and analyzed the association between miR­126 and various clinicopathological parameters. In addition, cell proliferation, wound healing and cell invasion assays were conducted using RCC cells overexpressing miR­126. Potential miR­126 target genes and the signaling pathways that may be regulated by miR­126 were then examined. miR­126 expression was significantly reduced in patients with metastatic RCC compared with patients without metastasis. Consistently, overexpression of miR­126 in RCC cells significantly inhibited cell proliferation, migration and invasion in vitro compared with negative control miRNA. A luciferase reporter assay demonstrated that miR­126 targets Rho associated coiled­coil containing protein kinase 1 (ROCK1) by directly binding the 3'­untranslated region. Furthermore, western blotting identified miR­126 as an important regulator of the AKT and extracellular signal­regulated 1/2 signaling pathways. The results of the present study indicate that miR­126 inhibits RCC cell proliferation, migration and invasion by downregulating ROCK1. These findings suggest that miR­126 may be valuable as a potential target for therapeutic intervention in RCC.


Assuntos
Carcinoma de Células Renais/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Renais/genética , MicroRNAs/genética , Interferência de RNA , Quinases Associadas a rho/genética , Regiões 3' não Traduzidas , Idoso , Idoso de 80 Anos ou mais , Sítios de Ligação , Carcinoma de Células Renais/diagnóstico , Carcinoma de Células Renais/metabolismo , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células , Regulação para Baixo , Feminino , Expressão Gênica , Humanos , Neoplasias Renais/diagnóstico , Neoplasias Renais/metabolismo , Sistema de Sinalização das MAP Quinases , Masculino , Pessoa de Meia-Idade , Gradação de Tumores , Invasividade Neoplásica , Estadiamento de Neoplasias , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
12.
Medicine (Baltimore) ; 95(16): e3393, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27100425

RESUMO

This study investigated the effectiveness of a new strategy, repeated radiofrequency (RF) ablation combined with ablated lesion elimination following transarterial chemoembolization (TACE)/transarterial embolization (TAE), for solitary huge hepatocellular carcinoma (SHHCC) 10 cm or larger. From July 2008 to October 2015, 39 consecutive patients with SHHCC were screened. Of these, 12 were treated with TACE/TAE and repeated RF ablation (TACE/TAE + RF ablation group) and the remaining 27 patients were treated with the aforementioned new strategy (new strategy group). Local tumor progression (LTP)-free survival, intrahepatic distant recurrence (IDR)-free survival, and overall survival (OS) rates were obtained using the Kaplan-Meier method. Univariate and multivariate analyses were performed on several clinicopathological variables to identify factors affecting long-term outcome and intrahepatic recurrence. Correlation analysis was also performed. The 1-, 2-, and 3-year LTP-free survival rates and OS rates were significantly higher in the new strategy group than in the TACE/TAE + RF ablation group (82.9% vs 58.3%, 73.9% vs 29.2%, 18.5% vs 9.7%, P = 0.002; 92.0% vs 75.0%, 84.0% vs 33.3%, 32.7% vs 16.7%, P = 0.025). However, there was no significant difference between the 2 groups in the 1-, 2-, and 3-year IDR-free survival rates (P = 0.108). Using univariate analysis, alpha-fetoprotein (AFP > 200 ng/mL), ablative margin (AM > 1.0 cm), and well-differentiated cells were found to be significant factors for predicting LTP, IDR, and OS. Surgical elimination was found to be a significant factor only for predicting OS. In multivariate analyses, AFP (>200 ng/mL), AM (>1.0 cm), and well-differentiated cells were found to be significant independent factors linked to LTP, IDR, and OS. Correlation analysis indicated that AM > 1.0 cm was strongly associated with surgical elimination (P < 0.001, correlation coefficient = 0.877). For patients with SHHCC who were initially excluded from surgery, the new strategy including repeated RF ablation combined with ablated lesion elimination following TACE/TAE should now be considered as an alternative treatment.


Assuntos
Carcinoma Hepatocelular/terapia , Ablação por Cateter/métodos , Quimioembolização Terapêutica/métodos , Hepatectomia/métodos , Neoplasias Hepáticas/terapia , Estadiamento de Neoplasias/métodos , Carga Tumoral , Antineoplásicos/administração & dosagem , Carcinoma Hepatocelular/epidemiologia , Carcinoma Hepatocelular/patologia , China/epidemiologia , Intervalo Livre de Doença , Seguimentos , Injeções Intra-Arteriais , Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/patologia , Morbidade/tendências , Reoperação/estatística & dados numéricos , Estudos Retrospectivos , Taxa de Sobrevida/tendências , Resultado do Tratamento
13.
PLoS One ; 11(2): e0149755, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26901132

RESUMO

OBJECTIVES: The aim of this study was to evaluate the technical and clinical outcomes of using laparoscopic radiofrequency (RF) ablation for treating large subcapsular hepatic hemangiomas. METHODS: We retrospectively reviewed our sequential experience of treating 124 large subcapsular hepatic hemangiomas in 121 patients with laparoscopic RF ablation. RESULTS: The mean diameter of the 124 hemangiomas was 9.1 ± 3.2 cm (5.0-16.0 cm). RF ablation was performed successfully in all patients. There were 55 complications related to the ablation in 26 patients, including 5 of 69 (7.3%) patients with hemangioma <10 cm and 21 of 52 (40.4%) patients with hemangiomas ≥10 cm (P < 0.001). No injuries to abdominal viscera occurred in all the 121 patients. According to the Dindo-Clavien classification, all the complications were minor in 26 patients (Grade I). Out of 124 hepatic hemangiomas, 118 (95.2%) were completely ablated, including 70 of 72 (97.2%) lesions < 10 cm and 48 of 52 (92.3%) lesions ≥ 10 cm (P = 0.236). CONCLUSION: Laparoscopic RF ablation therapy is a safe, feasible and effective procedure for large subcapsular hepatic hemangiomas, even in the hepatic hemangiomas ≥ 10 cm. Its use avoids thermal injury to the abdominal viscera.


Assuntos
Ablação por Cateter , Hemangioma/cirurgia , Laparoscopia , Neoplasias Hepáticas/cirurgia , Adulto , Idoso , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
14.
Oncol Lett ; 9(5): 2163-2166, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-26137032

RESUMO

Sclerosing epithelioid fibrosarcoma (SEF) is a rare soft-tissue sarcoma for which there is no standardized treatment regimen available. The current treatment options for SEF are resection, radiation and chemotherapy. Surgical resection remains the mainstay of therapy for SEF. However, SEF is an aggressive tumor that is prone to repeated local recurrence if not widely excised. Radiation and chemotherapy are less commonly used due to the insensitivity of SEF to these therapies. The treatment of recurrent SEF is even more challenging. The present study describes a patient who presented with a giant recurrent SEF arising from the chest wall that was accompanied by emergent bleeding. The patient was a 70-year-old male who had multiple comorbid diseases, including hypertension and chronic cardiac dysfunction. A computed tomography (CT) scan indicated the involvement of the sternum and anterior mediastinum. However, the patient refused any further surgery. Subsequent to careful discussion and consideration, radiofrequency (RF) ablation and percutaneous iodine-125 implantation was administered. The emergent bleeding was successfully stopped and the tumor was eliminated using RF ablation. Percutaneous iodine-125 implantation under CT guidance established effective control on the growth of the tumor involving the mediastinum. Despite this, the tumor recurred 6 months after treatment. The patient refused any further treatment and was discharged. In conclusion, RF ablation and percutaneous permanent iodine-125 implantation is a feasible and safe salvage therapy for patients with recurrent SEF of the chest wall.

15.
World J Gastroenterol ; 21(19): 5941-9, 2015 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-26019459

RESUMO

AIM: To compare safety and therapeutic efficacy of laparoscopic radiofrequency (RF) ablation vs computed tomography (CT)-guided RF ablation for large hepatic hemangiomas abutting the diaphragm. METHODS: We retrospectively reviewed our sequential experience of treating 51 large hepatic hemangiomas abutting the diaphragm in 51 patients by CT-guided or laparoscopic RF ablation due to either the presence of symptoms and/or the enlargement of hemangioma. Altogether, 24 hemangiomas were ablated via a CT-guided percutaneous approach (CT-guided ablation group), and 27 hemangiomas were treated via a laparoscopic approach (laparoscopic ablation group). RESULTS: The mean diameter of the 51 hemangiomas was 9.6 ± 1.8 cm (range, 6.0-12.0 cm). There was no difference in the diameter of hemangiomas between the two groups (P > 0.05). RF ablation was performed successfully in all patients. There was no difference in ablation times between groups (P > 0.05). There were 23 thoracic complications in 17 patients: 15 (62.5%, 15/24) in the CT-guided ablation group and 2 (7.4%, 2/27) in the laparoscopic ablation group (P < 0.05). According to the Dindo-Clavien classification, two complications (pleural effusion and diaphragmatic rupture grade III) were major in two patients. All others were minor (grade I). Both major complications occurred in the CT-guided ablation group. The minor complications were treated successfully with conservative measures, and the two major complications underwent treatment by chest tube drainage and thoracoscopic surgery, respectively. Complete ablation was achieved in 91.7% (22/24) and 96.3% (26/27) in the CT-guided and the laparoscopic ablation groups, respectively (P > 0.05). CONCLUSION: Laparoscopic RF ablation therapy should be used as the first-line treatment option for large hepatic hemangiomas abutting the diaphragm. It avoids thermal injury to the diaphragm and reduces thoracic complications.


Assuntos
Ablação por Cateter/métodos , Hemangioma/cirurgia , Laparoscopia/métodos , Neoplasias Hepáticas/cirurgia , Radiografia Intervencionista/métodos , Cirurgia Assistida por Computador/métodos , Tomografia Computadorizada por Raios X , Adulto , Ablação por Cateter/efeitos adversos , Diafragma , Feminino , Hemangioma/diagnóstico por imagem , Hemangioma/patologia , Humanos , Laparoscopia/efeitos adversos , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Seleção de Pacientes , Complicações Pós-Operatórias/etiologia , Valor Preditivo dos Testes , Radiografia Intervencionista/efeitos adversos , Estudos Retrospectivos , Fatores de Risco , Cirurgia Assistida por Computador/efeitos adversos , Fatores de Tempo , Tomografia Computadorizada por Raios X/efeitos adversos , Resultado do Tratamento , Carga Tumoral
16.
World J Gastroenterol ; 21(17): 5287-94, 2015 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-25954102

RESUMO

AIM: To evaluate long-term outcomes of radiofrequency (RF) ablation as first-line therapy for single hepatocellular carcinoma (HCC) ≤ 3 cm and to determine survival and prognostic factors. METHODS: We included all 184 patients who underwent RF ablation as a first-line treatment for single HCC ≤ 3 cm between April 2005 and December 2013. According to the criteria of Livraghi, the 184 patients were divided into two groups: those suitable for surgical resection (84 cases) and those unsuitable for surgical resection (100 cases). The primary endpoints were the overall survival (OS) rate and safety; the secondary endpoints were primary technique effectiveness and recurrence rate. RESULTS: There were 19 (10.3%) cases of ablation related minor complications. The complete tumor ablation rate after one RF session was 97.8% (180/184). The rate of local tumor progression, extrahepatic metastases and intrahepatic distant recurrence were 4.9% (9/184), 9.8% (18/184) and 37.5% (69/184), respectively. In the 184 patients, the 1-, 3-, and 5-year OS rates were 99.5%, 81.0%, and 62.5%, respectively. The 1-, 3-, and 5-year OS rates were 100%, 86.9%, and 71.4%, respectively, in those suitable for surgical resection and 99.0%, 76.0%, and 55.0%, respectively, in those unsuitable for surgical resection (P = 0.021). On univariate and multivariate analyses, poorer OS was associated with Child-Pugh B class and portal hypertension (P < 0.05). CONCLUSION: RF ablation is a safe and effective treatment for single HCC ≤ 3 cm. The OS rate of patients suitable for surgical resection was similar to those reported in surgical series.


Assuntos
Carcinoma Hepatocelular/cirurgia , Ablação por Cateter , Neoplasias Hepáticas/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Hepatocelular/mortalidade , Carcinoma Hepatocelular/secundário , Ablação por Cateter/efeitos adversos , Ablação por Cateter/mortalidade , Distribuição de Qui-Quadrado , China , Progressão da Doença , Feminino , Humanos , Estimativa de Kaplan-Meier , Neoplasias Hepáticas/mortalidade , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Recidiva Local de Neoplasia , Razão de Chances , Seleção de Pacientes , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Fatores de Risco , Fatores de Tempo , Resultado do Tratamento , Carga Tumoral
17.
PLoS One ; 10(4): e0122956, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25849334

RESUMO

OBJECTIVES: The aim of this study was to investigate the geographic distribution of ß-globin gene mutations in different ethnic groups in Yunnan province. METHODS: From 2004 to 2014, 1,441 subjects with hemoglobin disorders, identified by PCR-reverse dot blot and DNA sequencing, were studied according to ethnicity and geographic origin. Haplotypes were examined among 41 unrelated thalassemia chromosomes. RESULTS: Eighteen ß-thalassemia mutations and seven hemoglobin variants were identified for 1,616 alleles in 22 different ethnic groups from all 16 prefecture-level divisions of Yunnan. The prevalence of ß-thalassemia was heterogeneous and regionally specific. CD 41-42 (-TCTT) was the most prevalent mutation in the populations of northeastern Yunnan. CD 17 (A>T) was the most common mutation in the populations of southeastern Yunnan, especially for the Zhuang minority, whereas Hb E (CD 26, G>A) was the most prevalent mutation in populations of southwestern Yunnan, especially for the Dai minority. Among the seven types of haplotypes identified, CD 17 (A>T) was mainly linked to haplotype VII (+ - - - - - +) and IVS-II-654 (C>T) was only linked to haplotype I (+ - - - - + +). CONCLUSION: Our data underline the heterogeneity of ß-globin gene mutations in Yunnan. This distribution of ß-globin mutations in the geographic regions and ethnic populations provided a detailed ethnic basis and evolutionary view of humans in southern China, which will be beneficial for genetic counseling and prevention strategies.


Assuntos
Globinas beta/genética , Talassemia beta/genética , Adolescente , Adulto , Pré-Escolar , China/epidemiologia , Análise Mutacional de DNA , Feminino , Frequência do Gene , Estudos de Associação Genética , Haplótipos , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prevalência , Adulto Jovem , Talassemia beta/epidemiologia
18.
Cell Biochem Biophys ; 72(3): 813-6, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25638340

RESUMO

To quantify myocardial glucose metabolism by (18)F-FDG PET/CT in patients that have coronary heart disease (CHD) according to traditional Chinese medicine classification. Ninety patients with CHD were enrolled and were categorized into three groups. All patients underwent PET-CT examination for (18)F-FDG uptake quantification. In group A, the radioactive signals were weak in multiple segments in 27 cases (90 %). One case had no visualization and two had normal visualization (mean SUV = 4 ± 0.6). In group B, the radioactive signals were in some local areas in eight cases (26.7 %). Twenty cases had an overall increase in signal density (SUV ≥ 8) (66.7 %). One case had no visualization, and one case had normal visualization (mean SUV 4 ± 0.6). In group C, 23 cases had no visual or a weak visual (SUV ≤ 2 ± 0.3) (76.7 %). Seven cases had segmental weak signals or signal defects. Different types of CHD demonstrate different metabolisms of myocardium glucose. It is necessary to dialectically classify CHD and apply differential treatment.


Assuntos
Doença da Artéria Coronariana/classificação , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Adulto , Doença da Artéria Coronariana/diagnóstico por imagem , Feminino , Fluordesoxiglucose F18 , Humanos , Classificação Internacional de Doenças , Masculino , Pessoa de Meia-Idade , Compostos Radiofarmacêuticos
19.
Appl Opt ; 53(24): 5448-52, 2014 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-25321118

RESUMO

A balanced plane-mirror heterodyne interferometer with a polarizing beam splitter used to recombine the reference and measurement beams is proposed to reduce periodic nonlinearity and to eliminate thermal error. Experimental results indicated that the periodic error due to ghost reflection was kept within ±36 pm, and the interferometer proposed was immune from thermal error.

20.
Water Sci Technol ; 70(6): 1025-31, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25259491

RESUMO

Industrial production of apparel consumes large quantity of freshwater and discharges effluents that intensify the problem of freshwater shortage and water pollution. The industrial water footprint (IWF) of a piece of apparel includes the water footprint (WF) of the fabric, apparel accessories (e.g. zipper, fastener, sewing thread) and industrial production processes. The objective of this paper is to carry out a pilot study on IWF accounting for three kinds of typical zipper (i.e. metal zipper, polyethylene terephthalate (PET) zipper and polyoxymethylene copolymer (Co-POM) zipper) that are commonly used for apparel production. The results reveal that product output exerts a remarkable influence on zipper's average IWF. Metal zipper has the largest IWF and followed by Co-POM zipper and PET zipper. Painting, dyeing and primary processing are the top three water-consuming processes and contribute about 90% of the zipper's IWF. Painting consumes the largest amount of freshwater among all processes and occupies more than 50% of the zipper's IWF. In addition, the grey water footprint (WFgrey) provides the greatest contribution, more than 80%, to the zipper's IWF. Based on these results, this paper also provides several strategies aimed at water economization and pollution reduction during industrial production of zipper.


Assuntos
Monitoramento Ambiental , Resíduos Industriais , Poluição da Água/análise , Água/química , Indústrias , Metais , Papel , Projetos Piloto , Eliminação de Resíduos Líquidos
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