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1.
Braz J Cardiovasc Surg ; 34(5): 525-534, 2019 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-31719006

RESUMO

OBJECTIVE: To evaluate the factors impacting on the conversion to sinus rhythm and on the postoperative rhythm findings in the six-month follow-up period of a mitral valve surgery combined with cryoablation Cox-Maze III procedure, in patients with atrial fibrillation. METHODS: In this study, we evaluated 80 patients who underwent structural valve disease surgery in combination with cryoablation. Indications for the surgical procedures were determined in the patients according to the presence of rheumatic or non-rheumatic structural disorders in the mitral valve as evaluated by echocardiography. Cox-Maze III procedure and left atrial appendix closure were applied. RESULTS: The results of receiver operating characteristics analysis indicated that the rate of conversion to the sinus rhythm was significantly higher in patients with left atrial diameters ≥ 45.5 mm and with ejection fraction (EF) ≥ 48.5%. However, the statistical differences disappeared in the sixth month. Thromboembolic (TE) events were seen only in three patients in the early period and no more TE events occurred in the six-month follow-up period. CONCLUSION: The EF and the preoperative left atrial diameter were determined to be the factors impacting on the conversion to sinus rhythm in patients who underwent mitral valve surgery in combination with cryoablation. Mitral valve surgery in combination with ablation for atrial fibrillation does not affect mortality and morbidity in the experienced health centers; however, it remains controversial whether it will provide additional health benefits to the patients compared to those who underwent only mitral valve surgery.


Assuntos
Fibrilação Atrial/fisiopatologia , Fibrilação Atrial/cirurgia , Criocirurgia/métodos , Frequência Cardíaca/fisiologia , Implante de Prótese de Valva Cardíaca/métodos , Valva Mitral/cirurgia , Adulto , Fibrilação Atrial/prevenção & controle , Eletrocardiografia , Feminino , Átrios do Coração/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Valva Mitral/fisiopatologia , Período Pós-Operatório , Período Pré-Operatório , Valores de Referência , Estudos Retrospectivos , Fatores de Risco , Sensibilidade e Especificidade , Fatores de Tempo , Resultado do Tratamento
2.
Mol Biol Rep ; 46(1): 841-845, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30600457

RESUMO

In this study, we aimed to determine the gene polymorphisms of antioxidant enzymes that determine or affect antioxidant activity in the occurrence of the disease and/or complications during and after the surgery in patients who were decided to undergo coronary artery bypass surgery due to coronary artery disease. Blood samples taken before operation in 26 coronary artery patients who were decided to be operated according to the international procedure and the phenol/chloroform method was used to isolate DNA. DNA samples were amplified by using polymerase chain reaction (PCR) method with specific primers for MnSOD, CAT, GPx-3 antioxidant gene regions. As a result of the increasing process, the PCR products for the purpose of determining gene polymorphism, NGOMIV SMA f and BSA I restriction enzymes were used for MNSOD, CAT and GPx-3 gene region, respectively. Allele frequencies were determined and compared by Chi square test. VV (46.15%) and VA (53.85%) genotype for MnSOD region, i TT (22.22%), TC (16.67%) and CC (61.11%) genotype for CAT region, and CC (12.50%), TC (25%) and TT (62.50%) genotypes for GPx-3 region were obtained. While there was no statistically significant significance in terms of genotypes obtained in MnSOD and GPx-3 gene regions (P > 0.05), a significant difference was found in the CAT gene region in terms of genotypes (P < 0.01). Although oxidative stress is important in relation to cardiovascular diseases and postoperative complications, virtually no study of antioxidant enzymes in gene polymorphism are included in the literature. Work is lacking in relation to the subject.


Assuntos
Catalase/genética , Doença da Artéria Coronariana/enzimologia , Doença da Artéria Coronariana/genética , Glutationa Peroxidase/genética , Polimorfismo Genético , Superóxido Dismutase/genética , Feminino , Humanos , Masculino
3.
Brachytherapy ; 18(2): 154-162, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30594422

RESUMO

PURPOSE: To evaluate the effect of metabolic parameters of pretreatment primary tumor and regional lymph nodes with F-18-FDG PET/CT compared with MRI findings for the prognostic value and disease-free survival (DFS) in locally advanced cervical cancer. MATERIAL AND METHODS: From 2011 to 2016, 112 patients with a diagnosis of cervical cancer stages IB2-IVA treated with concomitant chemoradiation therapy with 3D intracavitary brachytherapy were analyzed. From this group, 50 patients who underwent pretreatment and posttreatment FDG PET/CT and MRI were enrolled. LRFFS, DFS, and overall survival were analyzed in comparison with FDG PET/CT and MRI data. Relationship between SUVmax data and DFS was also assessed. RESULTS: The median followup was 21 months, and median age was 54 years. The estimated 5-year locoregional failure-free survival, DFS, and overall survival rates were 87.4%, 70%, and 81%, respectively. DFS was 59.5% in patients with nodal metastases in FDG PET/CT and 100% in node negative patients (p:0,017). DFS was 50% and 79.4% in MRI node-positive and in node-negative patients, respectively (p:0,260). In addition, the nodal SUVmax (p: 0.005) and posttreatment response in FDG PET-CT (p < 0.001) were significant prognostic factors for DFS. Furthermore, primary tumor volume in MRI (p:0,982), node positivity in MRI (p:0,301), and response in posttreatment MRI (p:0,26) are not significant prognostic factors for DFS. CONCLUSION: As a result, FDG PET/CT has higher accuracy than MRI in detecting lymph node metastasis, and tumor volume reduction on FDG PET/CT images was greater than that on MRI images after CCRT.


Assuntos
Linfonodos/diagnóstico por imagem , Imagem por Ressonância Magnética , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Neoplasias do Colo do Útero/diagnóstico por imagem , Neoplasias do Colo do Útero/terapia , Adulto , Idoso , Braquiterapia , Quimiorradioterapia , Intervalo Livre de Doença , Feminino , Fluordesoxiglucose F18 , Humanos , Linfonodos/patologia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Valor Preditivo dos Testes , Prognóstico , Compostos Radiofarmacêuticos , Taxa de Sobrevida , Carga Tumoral , Neoplasias do Colo do Útero/patologia
4.
Oxid Med Cell Longev ; 2017: 8271376, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29527252

RESUMO

Objective: The aim of the study is to determine the oxidative status in on-pump and off-pump coronary artery surgery and contribute to possible surgical choices in clinical practices in accordance with the information obtained as a result of this study. Methods: 52 patients undergoing open heart surgery (26 patients in on-pump group and 26 patients in off-pump group) were included in the study. MDA, GPx, GSH, CAT, and SOD were investigated in blood samples. Results: In the on-pump group, it was determined that there were a significant increase in MDA level in the peroperative period compared to the preoperative and postoperative periods and a significant increase in GSH level in the postoperative period than in the preoperative period. Additionally, while there was a significant decrease in CAT activity in the postoperative period than in the peroperative period, there was a statistically significant increase in SOD enzyme activity in the postoperative period compared to the preoperative and peroperative periods. A statistically significant increase was observed in SOD enzyme activity in the postoperative period in on-pump compared to off-pump group. Conclusion: It is thought that this oxidative damage can be suppressed by administering a suitable antioxidant supplement in the preoperative and peroperative periods among patients undergoing the on-pump operation.


Assuntos
Ponte de Artéria Coronária , Vasos Coronários/metabolismo , Bombas de Infusão/estatística & dados numéricos , Antioxidantes/metabolismo , Catalase/sangue , Vasos Coronários/cirurgia , Feminino , Glutationa/sangue , Homeostase , Humanos , Masculino , Malondialdeído/sangue , Oxirredução , Estresse Oxidativo , Guias de Prática Clínica como Assunto , Superóxido Dismutase/sangue
5.
J Matern Fetal Neonatal Med ; 30(4): 386-396, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27052666

RESUMO

OBJECTIVE: To comprehensively review the available evidence and existing consensus reports and guidelines regarding the pregnancy and reproductive implications of the mosquito-transmitted Zika virus (ZIKV) infection. A primary focus was to provide pertinent information to aid clinicians in the management of pregnancies at risk for, exposed to, or with confirmed ZIKV infection. METHOD: An extensive literature review was performed using Pubmed. Practice guidelines and consensus reports were accessed from international, national, and professional organizations' websites. The clinical articles for ZIKV infection testing varied from case reports to small epidemiologic studies. RESULTS: A ZIKV epidemic has been declared in several countries in the Americas. Fifty-two travel-associated ZIKV infection cases have been reported throughout the USA (as of February 10, 2016). The consequences of congenital fetal/newborn ZIKV infection could potentially have devastating consequences including miscarriage, fetal death, and major anomalies such as microcephaly, brain and brain-stem defects, and long-term neurologic sequelae. While not definitive, current evidence suggests the existence of nonvector-borne transmission through sexual activity with an infected male partner. For women at risk for sexual transmission, condom use is advised, especially during pregnancy. CONCLUSION: While ZIKV infection appears to be a mild disease in the general population the potential consequences to the fetus and newborn could be profound. Management guidelines are currently evolving and will be significantly impacted as new evidence develops. It is therefore imperative that obstetric health-care providers keep abreast of this rapidly evolving information landscape that has so far characterized this outbreak.


Assuntos
Desenvolvimento Fetal , Transmissão Vertical de Doença Infecciosa/prevenção & controle , Complicações Infecciosas na Gravidez , Infecção por Zika virus/transmissão , Zika virus , Aedes , Animais , Feminino , Guias como Assunto , Humanos , Recém-Nascido , Mordeduras e Picadas de Insetos/prevenção & controle , Masculino , Microcefalia/prevenção & controle , Gravidez , Complicações Infecciosas na Gravidez/prevenção & controle , Complicações Infecciosas na Gravidez/virologia , Viagem , Infecção por Zika virus/complicações , Infecção por Zika virus/diagnóstico , Infecção por Zika virus/prevenção & controle
6.
Drug Chem Toxicol ; 40(1): 7-12, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27063898

RESUMO

In this study, the frog embryo teratogenesis assay (FETAX - Xenopus) technique was employed to evaluate the potential teratogenicity of the selective ß-adrenoceptor (AR) agonist, CL 316.243. In this context, CL 316.243 was applied to the South African clawed frog (Xenopus laevis) embryos. The media containing the CL 316.24-exposed embryos were monitored and changed/replaced once every 24 hours. Using FETAX, we determined the minimum concentrations to inhibit growth (MCIG) for CL 316.243. The 96-hour no observable adverse effect concentration (NOAEC), the 96-hour lowest observable adverse effect concentration (LOAEC), the 96-hour EC50 (malformation) and the 96-hour LC50 (lethal concentration) for mortality and malformation could not be determined because the used concentrations did not affect viability or the presence of abnormalities. On the other hand, the MCIG of CL 316.243 was determined as 1 mg/L. Our results demonstrated that CL 316.243 administration was associated with no of teratogenic and toxic effects. However, from first concentration we used (1 to 5 mg/L) length of embryos reduced significantly (p < 0.001) when compared to control of Xenopus embryos. Further studies should be conducted with different concentrations in order to investigate the optimal concentrations for treating preterm labor with these substances.


Assuntos
Agonistas de Receptores Adrenérgicos beta 3/toxicidade , Dioxóis/toxicidade , Embrião não Mamífero/efeitos dos fármacos , Desenvolvimento Embrionário/efeitos dos fármacos , Teratogênese , Animais , Relação Dose-Resposta a Droga , Dose Letal Mediana , Nível de Efeito Adverso não Observado , Testes de Toxicidade , Xenopus laevis
8.
J Enzyme Inhib Med Chem ; 32(1): 13-19, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27766908

RESUMO

A series of 4-phthalimidobenzenesulfonamide derivatives were designed, synthesized and evaluated for the inhibitory activities against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Structures of the title compounds were confirmed by spectral and elemental analyses. The cholinesterase (ChE) inhibitory activity studies were carried out using Ellman's colorimetric method. The biological activity results revealed that all of the title compounds (except for compound 8) displayed high selectivity against AChE. Among the tested compounds, compound 7 was found to be the most potent against AChE (IC50= 1.35 ± 0.08 µM), while compound 3 exhibited the highest inhibition against BuChE (IC50= 13.41 ± 0.62 µM). Molecular docking studies of the most active compound 7 in AChE showed that this compound can interact with both the catalytic active site (CAS) and the peripheral anionic site (PAS) of AChE.


Assuntos
Acetilcolinesterase/efeitos dos fármacos , Butirilcolinesterase/efeitos dos fármacos , Inibidores da Colinesterase/farmacologia , Simulação de Acoplamento Molecular , Ftalimidas/química , Sulfonamidas/farmacologia , Análise Espectral/métodos , Sulfonamidas/química
9.
Oxid Med Cell Longev ; 2016: 1783728, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28101295

RESUMO

Objective. The purpose of this study is to determine the changes in oxidative damage and antioxidant parameters in open heart surgeries with cardiopulmonary bypass (CPB) in preoperative and early postoperative periods. Methods. A total of three consecutive arterial blood samples were obtained from the patients in the study group, in preoperative, early postoperative, and postoperative periods, respectively. Oxidative damage indicator (MDA) and antioxidant indicators (GPx, GSH, CAT, and SOD) were examined. Results. A statistically significant increase was observed in MDA level in postoperative period compared to preoperative and early postoperative periods. GSH levels and CAT activities increased significantly in early postoperative and postoperative periods. Analyses revealed an increase in GPx and SOD enzyme activities only in the postoperative period. Conclusion. Even though the increase in MDA level was suppressed by the increased GSH level and CAT activity like in early postoperative period, efficiency can be brought for the increases in insufficient significant antioxidant parameters in postoperative period by administering antioxidant supplements to the patients and thus the increase in MDA in postoperative period can be significantly suppressed.


Assuntos
Antioxidantes/metabolismo , Ponte Cardiopulmonar , Radicais Livres/metabolismo , Idoso , Catalase/sangue , Feminino , Glutationa Peroxidase/sangue , Humanos , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/sangue
10.
Oncol Lett ; 9(3): 1482-1488, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25663935

RESUMO

Loss of Forkhead box P1 (FOXP1) protein expression confers a poor prognosis in sporadic and familial breast cancer patients, and the FOXP1 gene maps to a tumor suppressor locus at chromosome 3p14. Although correlation studies have indicated that FOXP1 has a role in tumor suppression, determination of the regulatory mechanism of FOXP1 is required to establish its function in breast cancer. It has previously been identified that FOXP1 is regulated by estrogen in breast cancer and that treatment with bisphenol A is effective for regulating the transformation of the normal human breast epithelial cell line, MCF-10F. In addition, FOXO-regulated activation of FOXP1 inhibits the apoptosis of MCF-10F cells following tamoxifen and Akt inhibitor VIII administration. The present study indicates that FOXP1 regulation occurs via a PI3K/Akt/p70S6 kinase (p70S6K) signaling pathway. Following treatment with wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K)/Akt, MCF7 and MDA-MB-231 breast cancer cells demonstrated decreased FOXP1 protein expression levels; this result was also observed in the small interfering (si)RNA silencing of Akt. By contrast, overexpression of Akt resulted in increased FOXP1 protein expression levels in the MDA-MB-231 cells compared with the control cell lysates. Furthermore, treatment with rapamycin, a specific inhibitor of the mammalian target of rapamycin/p70S6K cascade, resulted in decreased FOXP1 expression in the MCF7 cells, but not in the MDA-MB-231 cells, which were resistant to rapamycin-induced inhibition. In addition, silencing of p70S6K using siRNA produced a marked decrease in FOXP1 expression. These data indicate that FOXP1 protein expression is regulated by a PI3K/Akt/p70S6K signaling cascade in breast cancer.

11.
J Obstet Gynaecol Res ; 41(6): 926-31, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25546378

RESUMO

AIM: The aim of the study was to investigate the effectiveness of zofenopril in an experimental model of ovarian torsion in rats with histologic and biochemical assessments. MATERIAL AND METHODS: Experimental procedures were performed on 35 female rats (Wistar albino). Rats were randomly divided into five groups as: sham (sham operated, n = 7); vehicle group 1 (torsion-detorsion, n = 7) with 2 h ischemia and 2 h reperfusion; vehicle group 2 (torsion-detorsion, n = 7) with 2 h ischemia and 5 days' reperfusion; zofenopril group 1 (torsion-detorsion, n = 7) with 2 h ischemia, 2 h reperfusion and a signal dose of oral 15 mg/kg zofenopril; and zofenopril group 2 (torsion-detorsion, n = 7) with 2 h ischemia, 5 days' reperfusion and 5 days' oral 15 mg/kg zofenopril. A scoring of histopathologic evaluation was performed on the ovaries according to congestion, bleeding, edema, and cellular degeneration. Biochemical assessments included catalase, tissue malondialdehyde and protein carbonyl. RESULTS: Compared with the vehicle groups, histopathologic scores, tissue malondialdehyde and protein carbonyl levels, which reflect oxidative stress markers, were significantly lower in the zofenopril groups. Furthermore, catalase levels were significantly increased in the zofenopril group. CONCLUSION: Our study results revealed that zofenopril attenuates injury induced by ischemia-reperfusion on rat ovary.


Assuntos
Inibidores da Enzima Conversora de Angiotensina/uso terapêutico , Antioxidantes/uso terapêutico , Captopril/análogos & derivados , Doenças Ovarianas/prevenção & controle , Ovário/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Traumatismo por Reperfusão/prevenção & controle , Inibidores da Enzima Conversora de Angiotensina/administração & dosagem , Animais , Antioxidantes/administração & dosagem , Biomarcadores/metabolismo , Captopril/administração & dosagem , Captopril/uso terapêutico , Catalase/antagonistas & inibidores , Catalase/metabolismo , Relação Dose-Resposta a Droga , Feminino , Malondialdeído/antagonistas & inibidores , Malondialdeído/metabolismo , Doenças Ovarianas/etiologia , Doenças Ovarianas/metabolismo , Doenças Ovarianas/patologia , Ovário/irrigação sanguínea , Ovário/metabolismo , Ovário/patologia , Carbonilação Proteica/efeitos dos fármacos , Distribuição Aleatória , Ratos Wistar , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Torção Mecânica
12.
Reprod Sci ; 22(5): 545-50, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25305128

RESUMO

OBJECTIVE: The aim of the present study is to investigate the efficiency of colchicine in the experimental rat ovarian torsion model in the light of histological and biochemical data. STUDY DESIGN: A total of 35 Wistar albino female rats were randomly divided into 5 groups, group 1: (control-sham operated, n = 7); group 2: (torsion/detorsion, n = 7) 2 hours of ischemia and 2 hours of reperfusion; group 3: (torsion/detorsion, n = 7), 2 hours of ischemia and 5 days of reperfusion; group 4: (torsion/detorsion, n = 7) 2 hours of ischemia and 2 hours of reperfusion and a signal dose of oral 1 mL/kg colchicine; and group 5: (torsion/detorsion, n = 7), 2 hours of ischemia and 5 days of reperfusion and 5 days of oral 1 mg/kg colchicine. Histopathologic evaluation was performed by a scoring that assesses congestion, bleeding, edema, and cellular degeneration in the ovarian tissue. Catalase, tissue malondialdehyde (MDA), and protein carbonyl levels were calculated. RESULTS: The histopathologic scores, MDA, and protein carbonyl levels in the control and colchicine groups were significantly lower than groups 2 and 3 (P < .001). Catalase activities were significantly higher in the control and colchicine groups than in groups 2 and 3 (P < .001). The results of the histopathologic parameters and biochemical markers showed that protective effects of colchicine treatment persisted up to 5 days. CONCLUSION: Our study results revealed that colchicine reduced ovarian ischemia-reperfusion injury in experimental rat ovarian torsion model. As the ovarian detorsion is the first choice of the treatment modality in the early phase, antioxidant and anti-inflammatory treatment modalities like colchicine might be used to reduce ovarian ischemia-reperfusion injury.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Colchicina/farmacologia , Doenças Ovarianas/tratamento farmacológico , Ovário/efeitos dos fármacos , Traumatismo por Reperfusão/terapia , Anormalidade Torcional/tratamento farmacológico , Animais , Catalase/metabolismo , Citoproteção , Modelos Animais de Doenças , Feminino , Malondialdeído/metabolismo , Doenças Ovarianas/complicações , Doenças Ovarianas/metabolismo , Doenças Ovarianas/patologia , Ovário/irrigação sanguínea , Ovário/metabolismo , Ovário/patologia , Carbonilação Proteica , Ratos Wistar , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Fatores de Tempo , Anormalidade Torcional/complicações , Anormalidade Torcional/metabolismo , Anormalidade Torcional/patologia
13.
J Obstet Gynaecol Res ; 40(4): 1157-60, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24612336

RESUMO

Hydatid disease is an endemic infection which can affect any organ, mainly the liver and lungs. Peritoneal echinococcosis is usually known to occur secondary to hepatic hydatid cyst rupture into the peritoneal cavity. An isolated cyst in the pelvic cavity is considered as primary only when there are no other hydatid cysts. Herein, we report an isolated pelvic-cervical hydatid cyst which presented without any involvement of the other abdominal organs or lungs. Our patient, a 27-year-old woman with the primary complaints of dyspareunia and chronic pelvic pain, had thin-walled large cystic mass originating from the cervix, diagnosed by ultrasonography. She underwent surgery with the most likely initial diagnosis of exophytic fibroid with cystic degeneration. Gynecologists should be aware of the possibility of isolated primary hydatid cyst of the pelvic cavity and should consider this condition in the differential diagnosis of cystic pelvic masses, especially in areas where the disease is endemic.


Assuntos
Colo do Útero/diagnóstico por imagem , Equinococose/diagnóstico por imagem , Doenças Peritoneais/diagnóstico por imagem , Doenças do Colo do Útero/diagnóstico por imagem , Adulto , Albendazol/uso terapêutico , Anti-Helmínticos/uso terapêutico , Colo do Útero/parasitologia , Colo do Útero/cirurgia , Terapia Combinada , Cistos/diagnóstico por imagem , Diagnóstico Diferencial , Erros de Diagnóstico , Escavação Retouterina , Dispareunia/etiologia , Equinococose/parasitologia , Equinococose/fisiopatologia , Equinococose/terapia , Feminino , Humanos , Leiomioma/diagnóstico por imagem , Dor Pélvica/etiologia , Doenças Peritoneais/parasitologia , Doenças Peritoneais/fisiopatologia , Doenças Peritoneais/terapia , Resultado do Tratamento , Turquia , Ultrassonografia , Doenças do Colo do Útero/parasitologia , Doenças do Colo do Útero/fisiopatologia , Doenças do Colo do Útero/terapia , Neoplasias Uterinas/diagnóstico por imagem
14.
Vaccine ; 32(20): 2275-80, 2014 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-24631073

RESUMO

BACKGROUND: During the recent pandemic, Influenza A/H1N1 vaccine uptake remained far below the targeted rates. Associated factors regarding vaccine refusal in the general population have been reported in many studies, however the reasons behind refusals for asthmatic children have not yet been identified. We aimed to investigate Influenza A/H1N1 virus vaccine acceptance for children with asthma, to determine the attitudes and beliefs of parents concerning Influenza A/H1N1 disease and vaccine and to identify the association of asthma control parameters with vaccination. METHODS: The parents of asthmatic children aged 6-18 years participated in a cross-sectional survey study in three pediatric allergy outpatient clinics. The survey measured demographic factors, asthma control parameters, vaccination rates, and beliefs and attitudes regarding Influenza A/H1N1 vaccine. RESULTS: Of the 625 asthmatic children, 16.8% (n=105) were immunized with Influenza A/H1N1 and 45.7% (n=286) with seasonal influenza vaccine. Educational background of parents (p<0.001 and p=0.002, for father's and mother's educational level, respectively), previous vaccination with seasonal influenza (p<0.001), and having a family member vaccinated against Influenza A/H1N1 (p<0.001) had a significant influence on vaccine acceptance, while fear of side effects (88.6%) was the major parental reason for refusing the vaccine. Asthma control parameters had no influence on uptake of the vaccine. Physician recommendation (84.8%) was important in the decision-making process for immunization. The statement "Children with asthma should receive swine flu vaccine" increased the likelihood of being vaccinated [OR: 2.160, (95%CI 1.135-4.111), p=0.019]. CONCLUSION: Although asthmatic children are considered to be a high-priority group for Influenza A/H1N1 vaccination, we found low uptake of vaccine among our patients. Beliefs and attitudes rather than asthma control parameters influenced parental decisions for immunization. Understanding the underlying determinants for refusing the vaccine will help to improve vaccine campaigns in advance of a future outbreak.


Assuntos
Asma/epidemiologia , Conhecimentos, Atitudes e Prática em Saúde , Vacinas contra Influenza/uso terapêutico , Pais/psicologia , Vacinação/psicologia , Vacinação/estatística & dados numéricos , Adolescente , Criança , Estudos Transversais , Tomada de Decisões , Feminino , Humanos , Vírus da Influenza A Subtipo H1N1 , Influenza Humana/prevenção & controle , Masculino , Inquéritos e Questionários
15.
Ugeskr Laeger ; 175(24): 1716-9, 2013 Jun 10.
Artigo em Dinamarquês | MEDLINE | ID: mdl-23763933

RESUMO

Chikungunya fever is an acute febrile illness associated with severe, often debilitating polyarthralgias. The disease is caused by the Chikungunya virus (CHIKV), an arthropod-borne virus that is transmitted to humans primarily via the bite of an infected mosquito. Since a re-emergence of CHIKV in 2004 in the Indian Ocean islands, the virus has spread into novel locations such as Europe. In Italy, an outbreak occurred in 2007. A mutation in CHIKV (E1-A226V) appears to improve virus survival in Ae. albopictus and also increase its virulence. Further attention should be given the disease since it is emerging in Europe.


Assuntos
Infecções por Alphavirus , Vírus Chikungunya/isolamento & purificação , Aedes/virologia , Infecções por Alphavirus/diagnóstico , Infecções por Alphavirus/epidemiologia , Infecções por Alphavirus/terapia , Animais , Febre de Chikungunya , Europa (Continente)/epidemiologia , Humanos
16.
J Clin Lab Anal ; 27(1): 81-5, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23292909

RESUMO

BACKGROUND: The aim of this study was to determine the relation between iron deficiency anemia (IDA) and serum leptin, hepcidin, and ghrelin levels. METHODS: Thirty children with IDA and 28 healthy children between the ages of 6 months and 6 years admitted to our hospital were evaluated prospectively. IDA was diagnosed based on clinical and laboratory findings. All children with IDA were treated with iron II-glycine-sulphate complex for 3 months. Complete blood count; iron metabolism parameters; and serum leptin, hepcidin, and ghrelin levels were studied in all healthy children and in children with IDA before and after treatment. RESULTS: In children with IDA, the decrease seen in serum leptin levels after the iron treatment was not statistically significant. However, the increase seen in serum hepcidin levels after the iron treatment was statistically significant (P = 0.038). Hepcidin levels were significantly higher in children with IDA who received iron treatment compared to healthy children (P = 0.008). After the iron treatment, serum ghrelin levels in children with IDA were also significantly higher compared to the levels before treatment and healthy children (P = 0.019 and 0.000, respectively). CONCLUSION: Serum ghrelin and hepcidin levels increase with iron treatment in children with IDA. In view of the higher serum ghrelin and hepcidin levels after iron treatment when compared to pretreatment levels and the healthy children, we suggest that the iron treatment has an important role in serum hepcidin and ghrelin synthesis.


Assuntos
Anemia Ferropriva/sangue , Peptídeos Catiônicos Antimicrobianos/sangue , Grelina/sangue , Anemia Ferropriva/epidemiologia , Pré-Escolar , Feminino , Hemoglobinas/metabolismo , Hepcidinas , Humanos , Lactente , Ferro/administração & dosagem , Leptina/sangue , Masculino , Estudos Prospectivos , Estatísticas não Paramétricas
17.
Bosn J Basic Med Sci ; 11(4): 228-33, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22117829

RESUMO

In this study, we investigated the effects of salt loading on sympathetic pressor activity, cardiac autonomic activity, mean arterial pressure (MAP), heart rate (HR) and on the relations between them in anesthetized two-kidney, one clip (2K1C) hypertensive rats. We submitted rats to either renal artery clipping or sham operation. Distilled water or 0.5 % NaCl was given orally to the clipped and sham-operated control rats for 4 weeks. Then, MAP and HR differences between pre- and post- autonomic blockade were evaluated as indexes of sympathetic pressor and cardiac autonomic activity, respectively. The autonomic blockade decreased MAP to the similar levels in all groups (between 81.7±7.6 -87.3±7.1 mmHg). Sympathetic pressor activity was greater in the clipped rats than in its sham-operated controls only under salt loading (55.3±6.2 vs. 37.0±4.1 mmHg, p<0.05). Cardiac autonomic activity was, predominantly, sympathetic and more in the clipped group than in the sham-operated rats under distilled water (48.3±8.6 vs. 19.7±7.0 beats/min, p<0.05) but not under salt loading. Salt loading inverted the relationship between HR and cardiac autonomic activity in 2K1C hypertensive rats (r=-0.76, p=0.046 vs. r=0.89, p=0.019). These results suggest that salt loading may have augmented the effect of renovascular constriction on MAP by affecting the sympathetic pressor activity and the relation between cardiac autonomic activity and HR in 2K1C hypertensive rats.


Assuntos
Pressão Sanguínea/efeitos dos fármacos , Hipertensão Renovascular/fisiopatologia , Cloreto de Sódio na Dieta/farmacologia , Sistema Nervoso Simpático/efeitos dos fármacos , Inconsciência/fisiopatologia , Animais , Pressão Sanguínea/fisiologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Frequência Cardíaca/efeitos dos fármacos , Frequência Cardíaca/fisiologia , Hipertensão Renovascular/etiologia , Masculino , Ratos , Ratos Wistar , Artéria Renal/fisiopatologia , Instrumentos Cirúrgicos/efeitos adversos , Sistema Nervoso Simpático/fisiologia
18.
Theriogenology ; 75(6): 1057-66, 2011 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-21220154

RESUMO

Previous work has indicated that although activity of the HCO3⁻/Cl⁻exchanger (AE), which regulates intracellular alkalosis, is high in the germinal vesicle (GV) stage, the oocyte is inhibited as it progresses through meiotic maturation. In this study, we aimed to investigate the defense mechanisms against acidosis during the meiotic maturation stages. Intracellular pH (pH(i)) was recorded using a microspectrofluorometric technique, and Na(+)/H(+) (NHE) and Na(+)-dependent HCO3⁻/Cl⁻ exchanger (NDCBE) activity were determined by measuring the recovery rate from induced acidosis. Additionally, SLC9A1 (for NHE) and SLC4A8 (for NDCBE) gene transcription levels were determined by real-time PCR. The recovery rate of first meiotic prophase (GV) oocytes was high, but it decreased during the meiotic metaphase II (MII) stage in HCO3⁻-free medium; it became high again at the pronuclear zygote (PN) stage. Recovery rate was significantly inhibited by 5-(N-ethyl-N-isopropyl) amiloride and cariporide or in the absence of extracellular Na(+), implicating NHE, specifically NHE1 activity. Moreover, the level of SLC9A1 transcription correlated with the observed changes in NHE activity. The changes in NHE activity during meiotic maturation displayed a similar pattern to that of AE. The recovery rate from acidosis was significantly higher in MII stage oocytes and PN zygotes in HCO3⁻-containing medium; however, the increase was significantly inhibited in Na(+)-free medium or 4,4'-diisocyanatostilbene-2,2'-disulfonic acid. Furthermore, changes in the transcription of SLC4A8 during meiotic maturation were concordant with the level of exchanger activity. These results indicate that NDCBE activity is present in mouse oocytes and zygotes, and that this activity exhibits a different pattern than that of AE and NHE during meiotic maturation.


Assuntos
Meiose/fisiologia , Oócitos/citologia , Animais , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Proteínas de Transporte de Cátions/fisiologia , Antiportadores de Cloreto-Bicarbonato/fisiologia , Feminino , Homeostase , Concentração de Íons de Hidrogênio , Camundongos , Camundongos Endogâmicos BALB C , Oócitos/efeitos dos fármacos , RNA Mensageiro/metabolismo , Simportadores de Sódio-Bicarbonato/genética , Simportadores de Sódio-Bicarbonato/metabolismo , Simportadores de Sódio-Bicarbonato/fisiologia , Trocador 1 de Sódio-Hidrogênio , Trocadores de Sódio-Hidrogênio/genética , Trocadores de Sódio-Hidrogênio/metabolismo , Trocadores de Sódio-Hidrogênio/fisiologia , Zigoto/citologia , Zigoto/metabolismo , Zigoto/fisiologia
19.
Clin Exp Nephrol ; 14(1): 22-7, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19789943

RESUMO

BACKGROUND: Nonsteroidal anti-inflammatory drugs act by inhibiting the rate-limiting enzymes cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2), which are important in prostanoid formation. The aim of this experimental study was to examine the effects of selective Cox-2 inhibitor, rofecoxib, with or without furosemide, on urine and serum electrolytes, creatinine clearance, plasma renin activity (PRA), and Cox-2 expression in the renal cortex. METHODS: Forty male Wistar albino rats were randomized into four groups, group 1, group 2, group 3, and group 4, and were treated with placebo, furosemide (20 mg/kg), rofecoxib (10 mg/kg) plus furosemide (12 mg/kg), and rofecoxib (10 mg/kg), respectively, and followed for 7 days. Body weights were measured daily. Urine osmolality and volume, and serum and urinary creatinine, sodium (Na(+)), and potassium (K(+)) were measured. Renal cortical Cox-2 protein expression was examined by immunohistochemical method. RESULTS: Compared with groups 1 and 3, body weights were significantly reduced in groups 2 and 4 (16.2 and 19.8 g, respectively; P < 0.05 for all). Urine volume in group 2 increased significantly compared with groups 1, 3, and 4 (P < 0.001, P < 0.008, and P < 0.004, respectively). Urine osmolality in group 2 decreased significantly compared with groups 1 and 3 (P < 0.05 for all). Blood urea nitrogen, serum creatinine and sodium, creatinine clearance, and 24-h urine Na(+) and K(+) levels were similar in all groups. Serum K(+) level was lowest in group 2, and there was a statistically significant difference between groups 2 and 4 (P < 0.05). Plasma renin activity was similar in all groups (P > 0.05). Renal cortical Cox-2 protein expression was lowest in group 1 and was significantly different from the other groups (P < 0.01 for all). The relationship between Cox-2 expression and plasma renin activity was not significant in any group (P > 0.05, r(2):0.05). CONCLUSIONS: Rofecoxib neutralized the diuretic effect of furosemide in rats treated with a combination of furosemide and rofecoxib. Renal cortical Cox-2 protein expressions due to furosemide and rofecoxib with or without furosemide were similar and significantly increased compared with controls. Renal failure due to rofecoxib did not developed in any rat, but selective Cox-2 inhibitor, rofecoxib, might have similar renal effects as nonselective nonsteroidal drugs for blunting the diuretic effect of furosemide.


Assuntos
Inibidores de Ciclo-Oxigenase 2/farmacologia , Ciclo-Oxigenase 2/biossíntese , Furosemida/farmacologia , Rim/fisiologia , Lactonas/farmacologia , Sulfonas/farmacologia , Animais , Inibidores de Ciclo-Oxigenase 2/administração & dosagem , Furosemida/administração & dosagem , Expressão Gênica/efeitos dos fármacos , Rim/efeitos dos fármacos , Lactonas/administração & dosagem , Masculino , Concentração Osmolar , Potássio/sangue , Ratos , Ratos Wistar , Renina/sangue , Sulfonas/administração & dosagem , Urina/fisiologia
20.
J Sports Sci Med ; 9(2): 294-9, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-24149699

RESUMO

Diabetic nephropathy is a feared complication of diabetes since it can lead to end-stage renal failure and also it is a risk factor of cardiovascular disease. The important clinical problems caused by diabetic nephropathy are proteinuria and decreased renal function. Exercise is a cornerstone of diabetes management, along with diet and medication. Since acute exercise causes proteinuria and decreases glomerular filtration rate, the effect of exercise on diabetic nephropathy is controversial. The aim of this study was to investigate the effect of regular aerobic exercise on microalbuminuria and glomerular filtration rate in diabetic rats. Moderate diabetes was induced by streptozotocin (45 mg/kg IV) in rats and an aerobic exercise- training program on a treadmill was carried out for 8 weeks. Four groups of rats; control sedentary (CS), control exercise (CE), diabetic sedentary (DS) and diabetic exercise (DE) were included in the study. Blood glucose levels were determined from the plasma samples taken at the end of 4 weeks of stabilization period and 8 weeks of training program. Creatinine clearance (CCr) and microalbuminuria (MA) levels were determined to evaluate renal functions. The analyzed data revealed that regular aerobic exercise: 1) significantly decreased the plasma glucose level of the DE group compared to the DS group (p < 0.05), 2) significantly decreased the microalbuminuria level of the DE group compared to those of DS group (p < 0.01), 3) significantly decreased the creatinine clearance levels of the DE and CE groups compared to those of CS group (p < 0.05). The results of this study suggest that despite of decreasing creatinine clearance, regular submaximal aerobic exercise has a preventive effect on development of microalbuminuria and thus may retard nephropathy in diabetic rats. Key pointsRegular submaximal aerobic exercise can facilitate the control of blood glucose level in diabetic rats.Streptozotocin induced diabetes may cause microalbuminuria and regular submaximal aerobic exercise may have a preventive effect on renal functions.

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