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1.
Przegl Epidemiol ; 65(2): 199-203, 2011.
Artigo em Polonês | MEDLINE | ID: mdl-21913459

RESUMO

A total number of 1,081,974 cases of influenza and influenza-like illness were registered in Poland in 2009 (incidence 2,835.9 per 100,000 population). It was nearly 5 times more than in 2008. The impact on increase of the number of reported cases have had two factors: the pandemic of influenza caused by virus A(H1N1)v, and increasing of the surveillance sensitivity. 3,177 (0.29%) cases was laboratory confirmed. In the area of particular regions incidence ranged from 805.2 in swietokrzyskie voivodeship to 5,257.9 in warmifisko-mazurskie voivodeship. Nearly 37% of cases were children under 15 years. The incidence in this age group was 6,851.2 (from 2,010.1 in Swietokrzyskie voivodeship, to 13,291.6 in warminsko-mazurskie voivodeship). The highest reported incidence was observed in age group 5-14 years (7,135.2). To hospitals, mainly for epidemiological reasons, 8,944 people were sent (0.83% all cases). According to Central Statistical Office data, there were 87 death cases, including 8 (9.2%) children in the age of 15. 70.1% of deaths were registered as cases caused by identified influenza virus.


Assuntos
Surtos de Doenças/estatística & dados numéricos , Influenza Humana/diagnóstico , Influenza Humana/epidemiologia , Sistema de Registros/estatística & dados numéricos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Incidência , Lactente , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Vacinas contra Influenza/administração & dosagem , Influenza Humana/prevenção & controle , Influenza Humana/virologia , Influenzavirus A/isolamento & purificação , Masculino , Vacinação em Massa/estatística & dados numéricos , Pessoa de Meia-Idade , Polônia/epidemiologia , População Rural/estatística & dados numéricos , População Urbana/estatística & dados numéricos , Adulto Jovem
2.
J Clin Microbiol ; 49(6): 2216-21, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21471335

RESUMO

Mixed infections of a single host with different variants of influenza A virus are the main source of reassortants which may have unpredictable properties when they establish themselves in the human population. In this report we describe a method for rapid detection of mixed influenza virus infections with the seasonal A/H1N1 human strain and the pandemic A/H1N1/v strain which emerged in 2009 in Mexico and the United States. The influenza virus A/H1N1 variants were characterized by the multitemperature single-stranded conformational polymorphism (MSSCP) method. The MSSCP gel patterns of hemagglutinin gene fragments of pandemic A/H1N1/v and different seasonal A/H1N1 strains were easily distinguishable 2 h after completion of reverse transcription-PCR (RT-PCR). Using the MSSCP-based genotyping approach, coinfections with seasonal and pandemic variants of the A/H1N1 subtype were identified in 4 out of 23 primary samples obtained from patients that presented with influenza-like symptoms to hospitals across Poland during the 2009-2010 epidemic season. Pandemic influenza virus strain presence was confirmed in all these primary samples by real-time RT-PCR. The sensitivity level of the MSSCP-based minor genetic variant detection was 0.1%, as determined on a mixture of DNA fragments obtained from amplification of the hemagglutinin gene of seasonal and pandemic strains. The high sensitivity of the method suggests its applicability for characterization of new viral variants long before they become dominant.


Assuntos
Vírus da Influenza A Subtipo H1N1/classificação , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Influenza Humana/diagnóstico , Influenza Humana/virologia , Polimorfismo Conformacional de Fita Simples , Virologia/métodos , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Genótipo , Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética , Humanos , Vírus da Influenza A Subtipo H1N1/genética , Masculino , Pessoa de Meia-Idade , Polônia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Sensibilidade e Especificidade , Adulto Jovem
3.
Przegl Epidemiol ; 64(2): 175-9, 2010.
Artigo em Polonês | MEDLINE | ID: mdl-20731217

RESUMO

A total number of 227,346 cases of influenza and influenza-like illness were registered in Poland in 2008 (incidence 596.5 per 100,000 population). Compared to 2007 on 39.2% incidence decrease was observed. Regionally the incidence varied from 142.3 in swietokrzyskie voivodeship to 1,830.6 in opolskie. Children and adolescents under 15 years of age accounted for 36% of all cases (age group incidence 1,415.6). In this age group the incidence varied regionally from 342.9 in swietokrzyskie voivodeship to 4,083.6 in opolskie. The highest reported incidence was observed in age group 0-4 years (1,545.6). 153 patients (0.07% of all cases) required hospital admission. There were 16 deaths due to influenza, of which 11 (68.8%) were among persons over 70 years of age. In the epidemic season 2007/08 infections with influenza virus registered in Poland were caused by type A (60% of laboratory confirmed influenza cases) as well as by type B (40% of laboratory confirmed influenza cases), similarly to other parts of Europe. Forty-four influenza strains were isolated, including 24 strains of subtype A/H1 and 20 strains of type B. All of them were antigenically similar to the vaccine strains recommended for the epidemic season 2007/2008.


Assuntos
Surtos de Doenças/estatística & dados numéricos , Vacinas contra Influenza/administração & dosagem , Influenza Humana/diagnóstico , Influenza Humana/epidemiologia , Sistema de Registros/estatística & dados numéricos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Incidência , Lactente , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H3N2/isolamento & purificação , Influenza Humana/prevenção & controle , Influenza Humana/virologia , Influenzavirus A/isolamento & purificação , Masculino , Vacinação em Massa/estatística & dados numéricos , Pessoa de Meia-Idade , Polônia/epidemiologia , População Rural/estatística & dados numéricos , População Urbana/estatística & dados numéricos
4.
J Biol Inorg Chem ; 15(3): 429-40, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20091072

RESUMO

The synthesis and characterization of three novel iridium(III) complexes and one rhodium(III) complex with 1-nitroso-2-naphthol (3) chelating as a 1,2-naphthoquinone-1-oximato ligand are described. The reaction of mu(2)-halogenido-bridged dimers [(eta(5)-C(5)Me(5))IrX(2)](2) [X is Cl (1a), Br (1b), I (1c)] and [(eta(5)-C(5)Me(5))RhCl(2)](2) (2a) with 3 in CH(2)Cl(2) yields the mononuclear complexes (eta(5)-C(5)Me(5))IrX(eta(2)-C(10)H(6)N(2)O) (4a, 4b, 4c) and (eta(5)-C(5)Me(5))RhCl(eta(2)-C(10)H(6)N(2)O) (5a). All compounds were characterized by their (1)H and (13)C NMR, IR, and mass spectra, UV/vis spectra were recorded for 4a and 5a. The X-ray structure analyses revealed a pseudo-octahedral "piano-stool" configuration for the metals with bidentate coordination through oximato-N and naphthoquinone-O, forming a nearly planar five-membered metallacycle. The metal complexes 4a and 5a were evaluated in respect to their cytotoxicity and binding affinity toward double-stranded DNA. As determined in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, both exerted a much stronger cytotoxic effect toward HeLa and HL60 cancer cell lines than did cisplatin. The remarkable cytotoxicity of the compounds tested may be attributed to necrosis, rather than to apoptosis, as it is evidenced by the caspase-3/7 activation assay. No clear evidence was found for interaction with double-stranded DNA. The melting experiments showed no significant differences between thermodynamic parameters of intact DNA and DNA incubated with 3, 4a, or 5a, although these derivatives altered DNA recognition by the BamHI restriction enzyme. Therefore, the screened iridium and rhodium complexes 4a and 5a may still be interesting as potential anticancer drugs owing to their high cytotoxicity toward cancer cell lines, whereas they do not modify DNA in a way similar to that of cisplatin.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Irídio/química , Irídio/farmacologia , Ródio/química , Ródio/farmacologia , Caspase 3/metabolismo , Caspase 7/metabolismo , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Células Cultivadas , Dicroísmo Circular , Complexos de Coordenação/química , Complexos de Coordenação/farmacologia , Cristalografia por Raios X , DNA/metabolismo , Células HeLa , Humanos , Modelos Moleculares , Naftoquinonas/química , Naftoquinonas/farmacologia , Neoplasias/tratamento farmacológico
5.
Folia Histochem Cytobiol ; 46(2): 153-7, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18519231

RESUMO

Mycobacterium bovis bacillus Calmette-Guérin (BCG) is a live vaccine that has been used in routine vaccination against tuberculosis for nearly 80 years. However, its efficacy is controversial. The failure of BCG vaccination may be at least partially explained by the induction of poor or inappropriate host responses. Dendritic cells (DCs) are likely to play a key role in the induction of immune response to mycobacteria by polarizing the reactivity of T lymphocytes toward a Th1 profile, contributing to the generation of protective cellular immunity against mycobacteria. In this study we aimed to investigate the production of Th1 and Th2 cytokines by naive CD4+ T cells to mycobacterial antigen-pulsed DCs in the group of young, healthy BCG vaccinated volunteers. The response of naive helper T cells was compared with the response of total blood lymphocytes. Our present results clearly showed that circulating naive CD45RA+CD4+ lymphocytes from BCG-vaccinated subjects can become effector helper cells producing IFN-gamma and IL-5 under the stimulation by autologous dendritic cells presenting mycobacterial protein antigen-PPD or infected with live M. bovis BCG bacilli.


Assuntos
Antígenos de Bactérias/imunologia , Vacina BCG/imunologia , Células Dendríticas/imunologia , Interferon gama/biossíntese , Interleucina-5/biossíntese , Mycobacterium/imunologia , Linfócitos T Auxiliares-Indutores/imunologia , Adulto , Vacina BCG/farmacologia , Células Dendríticas/efeitos dos fármacos , Humanos , Lipopolissacarídeos/farmacologia , Mycobacterium/efeitos dos fármacos , Linfócitos T Auxiliares-Indutores/efeitos dos fármacos , Tuberculina/imunologia , Tuberculina/farmacologia , Vacinação
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