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1.
J Hazard Mater ; 422: 126922, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34425433

RESUMO

Phthalate esters (PAEs) are a group of ubiquitous organic environmental contaminants. Engineered ferromanganese-bearing sludge-derived biochar (SDB), synthesized using one-step pyrolysis in the temperature range between 300 and 900 °C, was used to enable Fenton-like processes that decontaminated PAE-laden sediments. SDB was thoroughly characterized using scanning electron microscopyenergy-dispersive spectroscopy, transmission electron microscopy, Brunauer-Emmett-Teller surface area, thermogravimetric analysis, Raman spectroscopy, Fourier-transform infrared spectroscopy, electron paramagnetic resonance, X-ray photoelectron spectroscopy, and fluorescence excitation-emission matrix spectroscopy coupled with parallel factor analysis. The maximum PAE degradation was remarkable at 90% in 12 h at pH 6.0 in the presence of 1.7 g L-1 of SDB 900. The highly-effective PAE degradation was mainly attributed to the synergism between FeOx and MnOx, which strengthened the activation of percarbonate (PC) via electron transfer, hydroxy addition, and hydrogen abstraction through radical (HO•) and nonradical (1O2) oxidation mechanisms, thereby facilitating PAE catalytic degradation over SDB in real sediments, which clearly proved the efficacy of ferromanganese-bearing SDB and PC for the remediation of contaminated sediments. The cytotoxicity exhibited by human skin keratinocyte cells exposure to high SDB concentration (100-400 µg mL-1) for 24-48 h was low indicating insignificant cellular toxicity and oxidative damages. This study provides a new strategy for freshwater sludge treatment and reutilization, which enables a water-cycle-based circular economy and waste-to-resource recycling.

2.
J Hazard Mater ; 421: 126708, 2022 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-34352521

RESUMO

The level and distribution of 16 USEPA Polycyclic aromatic hydrocarbon (PAHs) in aquaculture farmed fish (Mugil cephalus and Oreochromis mossambicus) and shellfish (Corbicula fluminea Formosa and Meretrix lusoria) were determined in Taiwan and then assessed cancer and non-cancer risks for those consuming these kinds of seafood. Results indicated that C. fluminea Formosa accumulated the highest average concentration of total PAHs (43.0 ± 11.3 ng/g wet weight) while M. lusoria contained the lowest concentration (20.0 ± 5.8 ng/g) among all species. The low-molecular-weight PAHs were dominant for both fish and shellfish, which consistent with other studies. Notably, parts of high-molecular-weight PAHs were found in shellfish whereas that was little in fish. The calculated hazard quotients (HQ) of all PAHs were smaller than 1 and the incremental lifetime cancer risks (ILCR) for Benzo[a]pyrene were below 1 × 10-5, suggesting that PAHs in the collected seafood could pose a low hazard to residents. Although the results indicated that the studied seafood is safe for human consumption, children and seniors post relatively higher risks, suggesting that it needs to continue monitoring and control the PAHs concentration in seafood and the associated environments.


Assuntos
Hidrocarbonetos Policíclicos Aromáticos , Animais , Aquicultura , Criança , Monitoramento Ambiental , Contaminação de Alimentos/análise , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Lagoas , Medição de Risco , Alimentos Marinhos/análise , Taiwan
3.
Bioresour Technol ; 343: 126082, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34610427

RESUMO

Antibiotic sulfamethoxazole (SMX) has been commonly found in various water matrices, therefore effective decontamination method is urgently needed. Metal-free pristine coconut-shell-derived biochar (CSBC), synthesized by thermochemical conversion at 700 °C, was used for activating peroxymonosulfate (PMS), an oxidant, to degrade SMX, a sulfonamide antibiotic, in water. SMX degradation, maximized at 0.05 mM concentration, was 85% in 30 min at pH 5.0 in the presence of 150 mg L-1 of CSBC. Remarkably, SMX removal reached 99% in a chloride-rich CSBC/PMS system. SMX degradation was mainly attributed to the role of CSBC in enhancing PMS activation to produce combined radical (SO4•-/HO•) and nonradical (1O2) reaction pathways. The most abundant genus in the CSBC/PMS system was Methylotenera, which belonged to the Proteobacteria phylum. Thus, from a perspective of biowaste-to-resource recycling and circular bioeconomy view point, CSBC is a potential catalytic activator of PMS for the removal of sulfonamide antibiotics from aqueous environments.


Assuntos
Poluentes Químicos da Água , Água , Antibacterianos , Carvão Vegetal , Peróxidos , Sulfonamidas , Poluentes Químicos da Água/análise
4.
Chemosphere ; 287(Pt 1): 131960, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34438213

RESUMO

The present report investigates the various MoO3 morphologies prepared via different approaches such as morphologies are cubic sheet, ribbon, and hexagonal sheet. These prepared nanostructures are modified as a MoO3/Ni-F electrode used to detect hydrogen peroxide (H2O2). The influence of the morphology on the microstructural, morphological, electronic state, optical and electrochemical properties of MoO3 nanostructures are systematically studied. The recorded XRD spectra confirmed that the good crystalline nature with the orthorhombic crystal structure. The FESEM analysis shows that preparation approaches strongly influenced the MoO3 morphology. The elemental mapping and XPS analysis confirm the formation of MoO3. The obtained optical band gap values show that the MoO3 morphology-based bandgap values are 3.38, 3.17, and 2.94 eV. The modified MoO3/Ni-F electrode electrochemical impedance spectra show the CP-MoO3 has good conductivity. Moreover, the CP-MoO3/Ni-F electrode has a wide detection window, long-term stability, reproducibility, and a low detection limit is 1.2 µM. Hence, the CP-MoO3/Ni-F electrode electrochemical results suggest that the modified electrode has offered a good matrix for toxic contaminants sensing applications.


Assuntos
Peróxido de Hidrogênio , Nanoestruturas , Técnicas Eletroquímicas , Eletrodos , Reprodutibilidade dos Testes
5.
Chemosphere ; 287(Pt 1): 132014, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34523445

RESUMO

Biohydrogen and biosyngas are among the sustainable bioenergy products from biomass resources through gasification. Microwave-assisted gasification (MAG) is still a novel technology, but it is definitely a promising conversion technology to achieve a sustainable bioeconomy. Although this technology shows a massive potential to be fully implemented in the near future, the selectivity and efficiency of biohydrogen and syngas production still need enhancements and further research to secure a cost-effective and energy-efficient industrialization. This article comprehensively reviews the regular, microwave-induced plasma, and catalytic MAG systems in relation to their biohydrogen and biosyngas production, carbon conversion efficiency, and tar removal while discussing the significance of optimal operating conditions and considerations in the gasification system design. Several perspectives such as benefits, challenges, numerical simulations, and scalable opportunities are also explored to provide factual insights for further research and industrial application.


Assuntos
Carbono , Micro-Ondas , Biomassa , Catálise , Tecnologia
6.
Bioresour Technol ; : 126343, 2021 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-34780908

RESUMO

Bacterial cellulose (BC) represents a novel bio-origin nonomaterial with its unique properties having diverse applications. Increased market demand and low yield are the major reason for its higher cost. Bacteria belonging to Komagataeibacter sp are the most exploited ones for BC production. Development of a cost-effective bioprocess for higher BC production is desirable. Though static fermentation modes have been majorly employed for BC production using tray fermenters, agitated mode has also been employed successfully with air-lift fermenters as well as stirred tank reactors. Bioprocess advances in recent years has led BC production to an upper level; however, challenges of aeration requirement and labor cost towards the higher end is associated with static cultivation at large scale. We have discussed the bioprocess development for BC production in recent years along with the challenges associated and the path forward.

7.
Bioresour Technol ; : 126351, 2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34798257

RESUMO

In this study, biochar derived from brown algal Ascophyllum nodosum was synthesized through hydrothermal carbonization (HTC) coupling with ZnCl2 chemical activation and applied as a sustainable adsorbent for antibiotic removal from water exemplified by ciprofloxacin (CIP). Various surface analysis techniques such as Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and zeta potential were used to clarify the surface properties of prepared biochars. The adsorption performance of biochars was investigated using batch adsorption experiments with a variety of parameters (initial pH, ionic types, temperature and water matrixes). The application of prepared biochar in CIP removal showed a good result of adsorption capacity (150-400 mg g-1) in different conditions. Overall, algal biochars, as a product recycled from biowaste, demonstrated a novel and promising adsorbent for effective and sustainable method for removal of antibiotics from water.

8.
Bioresour Technol ; : 126415, 2021 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-34838977

RESUMO

The bioprocessing of lignocellulosic biomass to produce bio-based products under biorefinery setup is gaining global attention. The economic viability of this biorefinery would be inclined by the efficient bioconversion of all three major constituents of lignocellulosic biomass i.e. cellulose, hemicellulose, and lignin for value-added biochemicals and biofuels production. Although the lignocellulosic biorefinery setup has a clear value proposition, the commercial success at the industrial scale is still inadequate. This can be attributed mainly to irregular biomass supply chain, market uncertainties, and scale-up challenges. Global research efforts are underway by public and private sectors to get deeper market penetration. A comprehensive account of important factors, limitations, and propositions are worth consideration for the commercial success of lignocellulosic biorefineries. In this article, the importance of integration of lignocellulosic biorefineries with existing petrochemical refineries, the technical challenges of industrialization, SWOT analysis, and future directions have been reviewed.

9.
Chemosphere ; : 132577, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34662641

RESUMO

In this study, biochar derived from spent coffee grounds (SCGB) was used to adsorb norfloxacin (NOR) in water. The biochar properties were interpreted by analysis of the specific surface area, morphology, structure, thermal stability, and functional groups. The impacts of pH, NOR, and ion's present on SCGB performance were examined. The NOR adsorption mode of SCGB is best suited to the Langmuir model (R2 = 0.974) with maximum absorption capacity (69.8 mg g-1). By using a Response Surface Method (RSM), optimal adsorption was also found at pH of 6.26, NOR of 24.69 mg L-1, and SCGB of 1.32 g L-1. Compared with biochars derived from agriculture such as corn stalks, willow branches, potato stem, reed stalks, cauliflower roots, wheat straw, the NOR adsorption capacity of SCGB was 2-30 times higher, but less than 3-4 times for biochars made from Salix mongolica, luffa sponge and polydopamine microspheres. These findings reveal that spent coffee grounds biochar could effectively remove NOR from aqueous solutions. Approaching biochar derived from coffee grounds would be a promising eco-friendly solution because it utilizes solid waste, saves costs, and creates adsorbents to deal with emerging pollutants like antibiotics.

10.
Foods ; 10(10)2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34681412

RESUMO

Chondroitin sulfate (ChS) is usually used as an oral nutraceutical supplement, and has been popular in Asia, Europe, and United States for many years. In this study, a potential and sustainable source of ChS from jumbo squid (Dosidicus gigas) cartilage was explored; ultrasound-assisted extraction (UAE) was used to extract ChS from jumbo squid cartilage. The result of mass transfer coefficients based on Fick's law showed that UAE had higher mass transfer efficacy. The response surface methodology (RSM) combined with Box-Behnken design (BBD) was employed to evaluate the effects of the extraction parameters. The optimal conditions were extraction temperature of 52 °C, extraction time of 46 min, and NaOH concentration of 4.15%. The crude extract was precipitated by 50% ethanol, which obtained a purified ChS with 23.7% yield and 82.3% purity. The purified ChS measured by energy-dispersive X-ray spectroscopy (EDX) had a carbon to sulfur molar ratio of approximately 14:1. The FTIR, 1H, and 13C NMR confirmed jumbo squid ChS were present in the form of chondroitin-4-sulfate and chondroitin-6-sulfate, with a 4S/6S ratio of 1.62. The results of this study provide an efficient process for production and purification of ChS, and are significant for the development and utilization of ChS from jumbo squid cartilage in the nutrient food or pharmaceutical industries.

11.
Bioresour Technol ; 344(Pt A): 126171, 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34695586

RESUMO

The need to develop sustainable alternatives for pretreatment and hydrolysis of lignocellulosic biomass (LCB) is a massive concern in the industrial sector today. Breaking down of LCB yields sugars and fuel in the bulk scale. If explored under nanotechnology, LCB can be refined to yield high-performance fuel sources. The toxicity and cost of conventional methods can be reduced by applying nanoparticles (NPs) in refining LCB. Immobilization of enzymes onto NPs or used in conjugation with nanomaterials would instill specific and eco-friendly options for hydrolyzing LCB. Nanomaterials increase the proficiency, reusability, and stability of enzymes. Notably, magnetic NPs have bagged their place in the downstream processing of LCB effluents due to their efficient separation and cost-effectiveness. The current review highlights the role of nanotechnology and its particles in refining LCB into various commercial precursors and value-added products. The relationship between nanotechnology and LCB refinery is portrayed effectively in the present study.

12.
Molecules ; 26(17)2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34500721

RESUMO

Fish bones (FBs) are aquatic by-products that are sources of antioxidant-active peptides, calcium dietary supplements, and biomedical materials. Usually, fermentation of these by-products via microorganisms brings desirable changes, enhancing their value. This study investigates the value addition of FB when fermented with Monascus purpureus (MP) for different time intervals, such as 3 days (F3) and 6 days (F6). The results indicate that the soluble protein, peptide, amino acid and total phenol content, as well as the antioxidant capacity (DPPH, ABTS+ radical scavenging activity, and relative reducing power), of F3 and F6 were significantly increased after fermentation. Furthermore, the ROS contents of F3 and F6 were reduced to a greater extent than that of hydrogen peroxide (H2O2) in Clone-9 cells. The MMP integrity, as well as the SOD, CAT, and GPx activity, of F3 and F6 were also increased significantly compared to the H2O2 in Clone-9 cells. Notably, F3 and F6 displayed significant reductions in ROS content, as well as elevate, SOD activity and MMP integrity in Clone-9 cells, when compared with the native FB. These results indicate that the FBs fermented with MP for 3 days (F3), and 6 days (F6) have antioxidant capacity, with possible applications as natural food supplements.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34386921

RESUMO

A new electrode was constructed via the anodic electropolymerization of poly-(L-serine) (PLS) on an rGO-Nafion-modified glassy carbon electrode (GCE) for the detection of the emerging organic contaminant naproxen (NPX). The morphology, crystal phase, and surface elements of the electrode were investigated with SEM, TEM, XRD, Raman, ATR-FTIR, zeta potential, C-H-O, and XPS analyses. Results of the surface analysis showed a porous structure resembling graphene sheets inside the Nafion/GCE architecture. Various electrochemical parameters, including scan rate, pH, and NPX concentration, were studied to evaluate the performance of the electrode. The synergistic effect of PLS and rGO-Nafion greatly facilitated the catalytic oxidation of NPX on PLS/rGO-Nafion/GCE. Electrochemical NPX oxidation was a one-electron transfer and adsorption limited process. The optimal working potential was 0.92 V vs. Ag/AgCl. The oxidation current of NPX increased with the increase in the concentration of analyte and scan rate but decreased with pH. The modified electrode exhibited excellent linearity with respect to NPX concentration in the range of 4.3 to 87 µM and limit of detection of 0.23 µM (S/N = 3). The PLS/rGO-Nafion/GCE is a fast, sensitive, reliable, and economical electrode for the detection of NPX in water.

14.
Bioresour Technol ; 340: 125708, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34391187

RESUMO

Malachite green (MG) is used as fungicide/parasiticide in aquaculture, its persistence is detrimental as it exhibits carcinogenic effects to aquatic organisms. Bacterial laccase evaluated as the best enzyme at extreme condition for aquatic MG removal. Study aims to increase laccase concentration, CotA-laccase from Bacillus subtilis was cloned and overexpressed in Escherichia coli. Optimal catalysis for purified CotA-laccase were at pH 5.0, 60 °C, and 1 mM of (2,2-azino-di-[3-ethylbenzothiazoline-sulphonate-(6)]) with Km and Kcat 0.087 mM and 37.64 S-1 respectively. MG biodegradation by CotA-laccase in clam and tilapia pond wastewaters and cytotoxic effect of biodegraded products in grouper fin-1 cells were determined. MG degradation by CotA-laccase was equally efficient, exhibiting upto 90-94% decolorization at freshwater and saline conditions and treated solution was non-toxic to GF-1 cells. Thus, recombinant-CotA-laccase could be an environmentally-friendly enzyme for aquaculture to remove MG, thereby effective to reduce its accumulation in aquatic organisms and ensuring safe aquaculture products.


Assuntos
Lacase , Corantes de Rosanilina , Bacillus subtilis , Proteínas de Bactérias , Corantes , Escherichia coli/genética , Concentração de Íons de Hidrogênio , Lacase/genética , Corantes de Rosanilina/toxicidade
16.
Membranes (Basel) ; 11(8)2021 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-34436360

RESUMO

In order to overcome the challenges of low permeate flux (Jp) and the accompanying reverse solute flux (JS) during the forward osmosis (FO) membrane separation process, we synthesized four hybrid materials of polyacid-based organic compounds and incorporated them into the selective polyamide (PA) layer to make novel thin-film nanocomposite (TFN) FO membranes. The Jp and JS of each membrane were evaluated and used along with membrane selectivity (Jp/JS) as indicators of membrane separation performance. The fabricated and modified membranes were also characterized for ridge and valley surface morphologies with increasing hydrophilicity and finger-shaped parallel channels in the PSf substrate. Moreover, two highly hydrophilic nanoparticles of graphene oxide (GO) and titanium oxide (TiO2) were introduced with the hybrid materials for PA modification, which can further enhance the Jp of the TFN membranes. The highest Jp of the TFN membranes achieved 12.1 L/m2-h using 0.1% curcumin-acetoguanamine @ cerium polyacid (CATCP) and 0.0175% GO. The characteristic peaks of the hybrid materials were detected on the membrane surface using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, evidencing successful incorporation of the hybrid materials during membrane modification. Here, we present the novel TFN membranes using hybrid materials for separation applications. The reactions for synthesizing the hybrid materials and for incorporating them with PA layer are proposed.

17.
Nutrients ; 13(8)2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34444969

RESUMO

Lemon (Citrus limon) has antioxidant, immunoregulatory, and blood lipid-lowering properties. This study aimed to determine the effect of the lemon fermented product (LFP) which is lemon fermented with Lactobacillus OPC1 to prevent obesity. The inhibition of lipid accumulation in 3T3-L1 adipocytes is examined using a Wistar rat model fed a high-fat diet to verify the anti-obesity efficacy and mechanism of LFP. Here, it was observed that LFP reduced cell proliferation and inhibited the lipid accumulation (8.3%) of 3T3-L1 adipocytes. Additionally, LFP reduced body weight (9.7%) and fat tissue weight (25.7%) of rats; reduced serum TG (17.0%), FFA (17.9%), glucose (29.3%) and ketone body (6.8%); and increased serum HDL-C (17.6%) and lipase activity (17.8%). LFP regulated the mRNA expression of genes related to lipid metabolism (PPARγ, C/EBPα, SREBP-1c, HSL, ATGL, FAS, and AMPK). Therefore, LFP reduces body weight and lipid accumulation by regulating the mRNA expression of genes related to lipid metabolism. Overall, our results implicate LFP as a potential dietary supplement for the prevention of obesity.


Assuntos
Fármacos Antiobesidade/farmacologia , Citrus/química , Dieta Hiperlipídica/efeitos adversos , Metabolismo dos Lipídeos/efeitos dos fármacos , Preparações de Plantas/farmacologia , Células 3T3-L1 , Adipócitos/efeitos dos fármacos , Animais , Modelos Animais de Doenças , Fermentação , Masculino , Camundongos , Obesidade/metabolismo , Ratos , Ratos Wistar
18.
J Biosci Bioeng ; 132(4): 359-364, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34389241

RESUMO

Macroalgae (seaweeds) are abundant in functional polysaccharides known for their unique biochemical activities. In this study, the antioxidant, anti-lipogenic, and anti-inflammatory activities of the fucoidan extracted from brown seaweed Sargassum siliquosum were investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging ability, lipid synthesis inhibition, and suppression of pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) production, respectively. To examine the effect of molecular mass on fucoidan's bioactivities above, the extracted fucoidan was subject to hydrogen peroxide-mediated partial hydrolysis to obtain lower molecular mass compounds within the range of 107.3-3.2 kDa. Results indicated that fucoidan's antioxidant activity increased with a corresponding decrease in molecular mass; the dosage for the half-maximal response (EC50) dropped from 2.58 to 1.82 mg/mL when the molecular mass decreased from 107.3 to 3.2 kDa. In addition, both the anti-lipogenesis and anti-inflammatory activities of fucoidan were significantly enhanced by 71.1% and 36.7%, respectively, when the molecular mass decreased to about 3 kDa. To further test the effect of sulfation on fucoidan's bioactivities, low molecular mass fucoidan was treated with SO3-DMF to increase the sulfate content. The results indicated that when sulfate content increased from 18.7% to 32.1%, EC50 of DPPH decreased from 1.82 mg/mL to 0.86 mg/mL and the anti-inflammatory activity also increased by 35.2%; however, the anti-lipogenesis activity decreased.


Assuntos
Sargassum , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Polissacarídeos/farmacologia , Sulfatos
19.
Chemosphere ; 281: 130796, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34289641

RESUMO

This paper describes the fabrication, modification, and evaluation of the performance of thin-film composite (TFC) forward osmosis (FO) membranes for lab-scale aquaculture wastewater recovery using various fumed silica (SiO2) nanoparticles. The active polyamide (PA) layers of these membranes were novelly modified using different types of pretreated SiO2 nanoparticles [virgin SiO2, dried SiO2, and 3-aminopropyltriethoxysilane (APTES)-modified SiO2] and concentrations (0.05, 0,1, 0,2, and 0.4 wt%) to improve the membrane hydrophilicity with minimum particle agglomeration. Results show that the APTES-SiO2 modified membrane had the highest water flux and selectivity, followed by the dried-SiO2 modified membrane. The APTES coupling agent notably reduced the SiO2 aggregation on the membrane surface and improved membrane hydrophilicity. Consequently, high permeate flux and an acceptable reverse solute flux were observed. The optimal SiO2 concentration for PA modification was 0.1 wt% for all the nanoparticle types. The virgin and APTES-SiO2 modified membranes were used for aquaculture wastewater recovery. The water recovery rate reached 47% in 84 h when using the APTES-SiO2 modified membrane, while it reached only 26% in 108 h when using the virgin membrane. With a suitable design of the filtration apparatus and choice of draw solution (DS), the prepared novel TFC-FO membrane containing APTES-modified SiO2 can be used for recycling aquaculture wastewater into the DS, which can then be reused for other purposes.


Assuntos
Nanopartículas , Purificação da Água , Aquicultura , Membranas Artificiais , Osmose , Dióxido de Silício , Águas Residuárias
20.
Environ Pollut ; 288: 117800, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34329062

RESUMO

There has been a significant increase in the microplastic (MP) polluting the ocean in recent time which is regarded as toxic for living organisms. In this study, Fluorescent red polyethylene microspheres (FRPE) were administered intramuscularly to Litopenaeus vannamei juveniles at the concentration of 0.1, 0.2, 0.5 and 1.0 µg (g shrimp)-1, and the survival rate was recorded. Analysis of the hepatopancreas for antioxidant enzyme activity and gene expression were done after seven days. Further tissue morphology and accumulation of FRPE was analysed. The results showed that FRPE at 0.5 and 1.0 µg (g shrimp)-1 reduce the survival rate of L. vannamei. FRPE at 0.5 and 1.0 µg (g shrimp)-1 reduced superoxide dismutase (SOD) activity; FRPE at different concentrations reduced catalase (CAT) activity; FRPE at 0.2, 0.5 and 1.0 µg (g shrimp)-1 increased the lipid peroxide thiobarbituric acid (TBARS) content. FRPE at 0.1, 0.2, and 0.5 µg (g shrimp)-1 significantly affect the performance of SOD and CAT genes; FRPE at 0.2 and 0.5 µg (g shrimp)-1 significantly improves GPx gene performance; FRPE at 1.0 µg (g shrimp)-1 significantly reduced the expression of GPx genes. Analysis of tissue morphology shows that FRPE cause muscle, midgut gland, and hepatopancreas, and gill damage at different concentrations. In the results of accumulation of microplastic, FRPE accumulated in gill tissue at 0.2 and 0.5 µg (g shrimp)-1; FRPE accumulated in gill, muscle and hepatopancreas tissue at 1.0 µg (g shrimp)-1. Based on the above results, FRPE at 0.5 and 1.0 µg (g shrimp)-1 can regulate the antioxidant enzymes of L. vannamei, increase lipid peroxide content, cause tissue damage by accumulating in the tissues. The rate of survival decreased in L. vannamei, and the impact of FRPE at 1.0 µg (g shrimp)-1 was significant.


Assuntos
Penaeidae , Plásticos , Animais , Microplásticos , Estresse Oxidativo , Penaeidae/genética , Polietileno
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