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1.
Zhongguo Dang Dai Er Ke Za Zhi ; 23(8): 802-808, 2021 Aug 15.
Artigo em Inglês, Chinês | MEDLINE | ID: mdl-34511169

RESUMO

OBJECTIVES: To study the association of methylenetetrahydrofolate reductase (MTHFR) gene polymorphism with susceptibility to bronchial asthma and glucocorticoid (GC) efficacy in children. METHODS: A total of 173 children with bronchial asthma who were hospitalized between June 2018 and December 2020 were selected as the observation group. The children received aerosol inhalation of GC for three consecutive months. A total of 178 healthy children who underwent physical examination during the same period were selected as the control group. PCR was used to detect the genotypes of the MTHFR C677T for the two groups. The differences in genotype distribution between the two groups were analyzed. Children with different genotypes in the observation group were compared in terms of immunoglobulin E (IgE), interleukin-8 (IL-8), leukotriene B4 (LTB4), lung function, and clinical outcome before and after treatment. RESULTS: TT genotype and T allele were significantly more frequent in the observation group than in the control group (P<0.001). TT/CT genotypes and T allele were independent risk factors for bronchial asthma (OR=6.615 and 7.055 respectively; P<0.001). After GC treatment, the children with CC, CT or TT genotypes experienced significantly decreased levels of IgE, IL-8, and LTB4 and significantly increased forced expiratory volume in 1 second (FEV1), forced vital capacity (FVC), and FEV1/FVC ratio (P<0.001). The children with TT genotype showed significantly lower levels of IL-8 and LTB4 than those with CC genotype, a significantly lower level of LTB4 than those with CT genotype, significantly higher FVC than those with CT genotype, and a significantly higher FEV1/FVC ratio than those with CC genotype (P<0.05). The children with TT genotype had better GC efficacy compared with those with CC genotype (P<0.05). TT genotype was an independent factor for good GC efficacy (OR=2.111, P=0.018). CONCLUSIONS: MTHFR gene polymorphism is associated with asthma susceptibility and GC efficacy in children. Children carrying TT/CT genotypes have a higher risk of developing asthma, and those with TT genotype are more sensitive to GC treatment.


Assuntos
Asma , Glucocorticoides , Alelos , Asma/tratamento farmacológico , Asma/genética , Criança , Predisposição Genética para Doença , Genótipo , Humanos , Metilenotetra-Hidrofolato Redutase (NADPH2)/genética , Polimorfismo Genético
3.
Ann Intensive Care ; 11(1): 16, 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33496906

RESUMO

BACKGROUND: Evaluation of fluid responsiveness during veno-arterial extracorporeal membrane oxygenation (VA-ECMO) support is crucial. The aim of this study was to investigate whether changes in left ventricular outflow tract velocity-time integral (ΔVTI), induced by a Trendelenburg maneuver, could predict fluid responsiveness during VA-ECMO. METHODS: This prospective study was conducted in patients with VA-ECMO support. The protocol included four sequential steps: (1) baseline-1, a supine position with a 15° upward bed angulation; (2) Trendelenburg maneuver, 15° downward bed angulation; (3) baseline-2, the same position as baseline-1, and (4) fluid challenge, administration of 500 mL gelatin over 15 min without postural change. Hemodynamic parameters were recorded at each step. Fluid responsiveness was defined as ΔVTI of 15% or more, after volume expansion. RESULTS: From June 2018 to December 2019, 22 patients with VA-ECMO were included, and a total of 39 measurements were performed. Of these, 22 measurements (56%) met fluid responsiveness. The R2 of the linear regression was 0.76, between ΔVTIs induced by Trendelenburg maneuver and the fluid challenge. The area under the receiver operating characteristic curve of ΔVTI induced by Trendelenburg maneuver to predict fluid responsiveness was 0.93 [95% confidence interval (CI) 0.81-0.98], with a sensitivity of 82% (95% CI 60-95%), and specificity of 88% (95% CI 64-99%), at a best threshold of 10% (95% CI 6-12%). CONCLUSIONS: Changes in VTI induced by the Trendelenburg maneuver could effectively predict fluid responsiveness in VA-ECMO patients. Trial registration ClinicalTrials.gov, NCT03553459 (the TEMPLE study). Registered on May 30, 2018.

4.
J Adv Nurs ; 77(4): 1813-1824, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33225451

RESUMO

AIMS: To determine psychological symptoms of patients with mild symptoms of coronavirus disease 2019 in China and to explore the influencing factors. DESIGN: A cross-sectional study. METHODS: A convenience sample of 296 mild coronavirus disease 2019 patients were recruited from a Fangcang hospital in Wuhan, Hubei Province, from 3-5 March, 2020. Participants were assessed using a sociodemographic and clinical characteristics questionnaire, and Symptom Check List 90. The binary logistic regression was utilized to explore the influencing factors of psychological symptoms of patients with mild symptoms of coronavirus disease 2019. RESULTS: In total, 296 of 299 patients with mild symptoms of coronavirus disease 2019 participated in the study (response rate: 99.0%). The findings revealed that 12.8% patients with mild symptoms have mental health problems; the most common psychological symptoms are phobic anxiety (58.4%), paranoid ideation (50.7%) and psychoticism (40.2%). Female patients [OR = 3.587, 95% CI (1.694-7.598)] and those having physical symptoms currently [OR = 2.813, 95% CI (1.210-6.539)] are at higher risk, while those in the middle duration of hospitalization [OR = 0.278, 95% CI (0.121-0.639)] protect against mental-health problems. CONCLUSIONS: The minority of patients with mild symptoms of coronavirus disease 2019 were still suffering from psychological symptoms. Healthcare providers are recommended to pay particular attention to screening these high-risk groups (women, those in the initial stages of hospitalization and those with physical symptoms currently) and implement targeted psychological care as required. IMPACT: This study found that most patients of coronavirus disease 2019 in Fangcang hospital exhibited normal mental health at par with the general Chinese norm and the minority of them were suffering from psychological symptoms. The findings can provide a reference for healthcare providers to screen high-risk psychological symptoms groups and implement targeted psychological intervention for patients with coronavirus disease 2019.


Assuntos
Transtornos de Ansiedade/etiologia , COVID-19/complicações , COVID-19/epidemiologia , COVID-19/psicologia , Transtorno Depressivo/etiologia , Avaliação de Sintomas/psicologia , Avaliação de Sintomas/estatística & dados numéricos , Adulto , Idoso , Idoso de 80 Anos ou mais , Transtornos de Ansiedade/epidemiologia , China/epidemiologia , Estudos Transversais , Transtorno Depressivo/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , Inquéritos e Questionários , Adulto Jovem
5.
J Hematol Oncol ; 13(1): 44, 2020 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-32366313

RESUMO

An amendment to this paper has been published and can be accessed via the original article.

6.
Artigo em Inglês | MEDLINE | ID: mdl-32121272

RESUMO

The dark adaptation of drivers' eyes at a tunnel entrance seriously affects traffic safety. This can be improved by the design of tunnel lighting. Light-Emitting Diode (LEDs) have been applied as a new type of luminaire in tunnel lighting in recent years, but at present, there are few studies on the influence of color rendering of LEDs on tunnel traffic safety, and there is no explicit indicator for the selection of appropriate color rendering parameters in tunnel lighting specifications, which has aroused researchers' concern. In this article, several new color rendering evaluation indexes were compared, and as a result, it is considered that CRI2012 (a color difference-based color rendering index) is more suitable for evaluating the color rendering of LEDs used at tunnel entrances. The dark adaptation phenomenon was simulated in the laboratory. Four CRI2012s, three color temperatures and eight colored targets were used in the experiments. The results showed that yellow, silver and white can provide shorter reaction times, while red and brown lead to longer reaction times, which can provide a reference for the design of road and warning signs at tunnel entrances. The effect of target color on reaction time was greater than that of color rendering. Under most target colors, the higher the CRI2012, the shorter the reaction time. When designing the color rendering of the LEDs at a tunnel entrance, the value should thus be as large as possible (close to 100), and a lower color temperature value (about 2800 K) should be selected. This paper provides technical support for tunnel lighting design and a reference for tunnel lighting specifications, which is of significance to improve driving safety and avoid traffic accidents in highway tunnels.


Assuntos
Condução de Veículo , Cor , Adaptação à Escuridão , Iluminação , Acidentes de Trânsito/prevenção & controle , Humanos
7.
Sci Total Environ ; 723: 137991, 2020 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-32213407

RESUMO

Biochar has been received increasing concerns regarding its environmental effect, which is promising in wastewater treatment. In this study, the performance of ß-cyclodextrin functionalized biochar (ß-BC) on the removal of antibiotic resistance genes (ARGs) in wastewater treatment under the co-stresses of heavy metals and dye is evaluated. Results show that when 20 mg/L heavy metals (HMs) and 150 mg/L methyl orange (MO) are present in daily fed influent, only 0.05 mg/L HMs residual and 96.79%-98.84% MO removal efficiency achieved in ß-BC additive group, compare to 0.16 mg/L and 87.92%-94.11% of that in control, respectively, indicating that ß-BC can benefits the performance of contaminants removal. To evaluate the role of ß-BC plays on ARGs in multi-contaminants stressed system, tet W, tet M, sul-1, sul-2, blaTEM, oxa-1, qnr-S, erm-B and intI-1 are identified. The relative abundance of all identified ARGs are decreased when ß-BC presence compared to the corresponding groups without ß-BC additive. The diversity and composition of microbial community are explored and the reduction of potential antibiotic-resistant bacteria is speculated as a driver of ARGs removal. In conclusion, our study demonstrates that ß-BC possesses the ability to promote the removal of ARGs during continuous wastewater treatment under HMs-MO co-contaminant.


Assuntos
Metais Pesados/análise , beta-Ciclodextrinas , Antibacterianos/farmacologia , Carvão Vegetal , Resistência Microbiana a Medicamentos/efeitos dos fármacos , Genes Bacterianos/efeitos dos fármacos , Águas Residuárias/análise
8.
Theranostics ; 10(8): 3668-3683, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32206115

RESUMO

Dysregulation of microRNA (miRNA) is a frequent event in hepatocellular carcinoma (HCC), but little is known whether it is a bystander or an actual player on residual HCC metastasis during liver microenvironment remodeling initiated by hepatectomy. Methods: The differently expressed miRNAs and mRNAs were identified from RNA-seq data. Western blot, qRT-PCR, fluorescence in situ hybridization, immunofluorescence and immunohistochemical were used to detect the expression of miRNA and mRNA in cell lines and patient tissues. The biological functions were investigated in vitro and in vivo. Chromatin immunoprecipitation, proximity ligation and luciferase reporter assay were used to explore the specific binding of target genes. The expression of HGF/ERBB3 signaling was detected by Western blot. Results: In this study, HGF induced by hepatectomy was shown to promote metastasis of residual HCC cells. miR-17-5p and miR-20a-5p were confirmed to play inhibitory roles on HCC metastasis. And ERBB3 was found to be the common target of miR-17-5p and miR-20a-5p. HCC cells with lower levels of miR-17-5p and miR-20a-5p or higher level of ERBB3 were often more sensitive to response HGF stimuli and to facilitate metastatic colonization both in vitro and in vivo experimental systems. Furthermore, HGF reinforced ERBB3 expression by NF-κB transcriptional activity in a positive feedback loop. Of particular importance, HCC patients with lower levels of miR-17-5p and miR-20a-5p or higher level of ERBB3 had significantly shorter OS and PFS survivals after surgical resection. Conclusion: miR-17-5p and miR-20a-5p could suppress postoperative metastasis of hepatocellular carcinoma via blocking HGF/ERBB3-NF-κB positive feedback loop and offer a new probable strategy for metastasis prevention after HCC resection.


Assuntos
Carcinoma Hepatocelular/metabolismo , Neoplasias Hepáticas/metabolismo , MicroRNAs/fisiologia , Metástase Neoplásica , Transdução de Sinais , Animais , Carcinoma Hepatocelular/cirurgia , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Estudos de Coortes , Feminino , Regulação Neoplásica da Expressão Gênica , Hepatectomia , Fator de Crescimento de Hepatócito/metabolismo , Humanos , Neoplasias Hepáticas/cirurgia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Pessoa de Meia-Idade , NF-kappa B/metabolismo , Receptor ErbB-3/metabolismo
9.
J Hematol Oncol ; 13(1): 12, 2020 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-32033570

RESUMO

BACKGROUND: MicroRNA-612 (miR-612) has been proven to suppress EMT, stemness, and tumor metastasis of hepatocellular carcinoma (HCC) via PI3K/AKT2 and Sp1/Nanog signaling. However, its biological roles on HCC progression are far from elucidated. METHODS: We found direct downstream target of miR-612, hadha by RNA immunoprecipitation and sequencing. To explore its biological characteristic, potential molecular mechanism, and clinical relevance in HCC patients, we performed several in-vitro and in-vivo models, as well as human tissue chip. RESULTS: Ectopic expression of miR-612 could partially reverse the level of HADHA, then suppress function of pseudopods, and diminish metastatic and invasive potential of HCC by lipid reprogramming. In detail, miR-612 might reduce invadopodia formation via HADHA-mediated cell membrane cholesterol alteration and accompanied with the inhibition of Wnt/ß-catenin regulated EMT occurrence. Our results showed that the maximum oxygen consumption rates (OCR) of HCCLM3miR-612-OE and HCCLM3hadha-KD cells were decreased nearly by 40% and 60% of their counterparts (p < 0.05). The levels of acetyl CoA were significantly decreased, about 1/3 (p > 0.05) or 1/2 (p < 0.05) of their controls, in exogenous miR-612 or hadha-shRNA transfected HCCLM3 cell lines. Besides, overexpression of hadha cell lines had a high expression level of total cholesterol, especially 27-hydroxycholesterol (p < 0.005). SREBP2 protein expression level as well as its downstream targets, HMGCS1, HMGCR, MVD, SQLE were all deregulated by HADHA. Meanwhile, the ATP levels were reduced to 1/2 and 1/4 in HCCLM3miR-612-OE (p < 0.05) and HCCLM3hadha-KD (p < 0.01) respectively. Moreover, patients with low miR-612 levels and high HADHA levels had a poor prognosis with shorter overall survival. CONCLUSION: miR-612 can suppress the formation of invadopodia, EMT, and HCC metastasis and by HADHA-mediated lipid programming, which may provide a new insight of miR-612 on tumor metastasis and progression.


Assuntos
Carcinoma Hepatocelular/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/genética , MicroRNAs/genética , Subunidade alfa da Proteína Mitocondrial Trifuncional/genética , Podossomos/genética , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Feminino , Humanos , Metabolismo dos Lipídeos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Podossomos/patologia
10.
J Hazard Mater ; 388: 122070, 2020 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954307

RESUMO

The emerging antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are increasingly appreciated to be as important as microbial contaminants. This paper focused on UV-activated persulfate (UV/PS), an advanced oxidation process, in removing ARB and ARGs from secondary wastewater effluent. Results showed that the inactivation efficiency of macrolides-resistant bacteria (MRB), sulfonamides-resistant bacteria (SRB), tetracyclines-resistant bacteria (TRB) and quinolones-resistant bacteria (QRB) by UV/PS reached 96.6 %, 94.7 %, 98.0 % and 99.9 % in 10 min, respectively. UV/PS also showed significant removal efficiency on ARGs. The reduction of total ARGs reached 3.84 orders of magnitude in UV/PS which is more than that in UV by 0.56 log. Particularly, the removal of mobile genetic elements (MGE) which might favor the horizontal gene transfer of ARGs among different microbial achieved 76.09 % by UV/PS. High-throughput sequencing revealed that UV/PS changed the microbial community. The proportions of Proteobacteria and Actinobacteria that pose human health risks were 4.25 % and 1.6 % less than UV, respectively. Co-occurrence analyzes indicated that ARGs were differentially contributed by bacterial taxa. In UV/PS system, hydroxyl radical and sulfate radical contributed to the removal of bacteria and ARGs. Our study provided a new method of UV/PS to remove ARGs and ARB for wastewater treatment.


Assuntos
Farmacorresistência Bacteriana/genética , Genes Bacterianos , Sulfatos/farmacologia , Raios Ultravioleta , Poluentes da Água , Purificação da Água/métodos , Antibacterianos , Bactérias/efeitos dos fármacos , Bactérias/efeitos da radiação , Macrolídeos , Quinolonas , Sulfonamidas , Tetraciclinas , Águas Residuárias
11.
J Exp Zool A Ecol Integr Physiol ; 333(4): 230-239, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31957988

RESUMO

Titanium dioxide nanoparticles (nTiO2 ) have raised environmental concerns and display potential hazards to aquatic organisms and even humans. However, only a few reports tested the toxicity of nTiO2 to rotifers. In the present study, acute and chronic toxicity of nTiO2 to the freshwater rotifer Brachionus calyciflorus was investigated at different temperatures. At 25°C, the 24 and 48-hr LC50 was 117.14 and 60.11 mg/L, respectively. At 15°C, 20°C, 25°C, and 30°C, exposure to nTiO2 significantly decreased life expectancy at birth, net reproductive rate, generation time, average lifespan, and/or intrinsic rate of population increase of B. calyciflorus (p < .05). High temperature enhanced the toxicity of nTiO2 to rotifers. The swimming linear speed of rotifers significantly increased (p < .05) in treatments with 200 µg/L nTiO2 , compared with the control. In addition, treatments with 8 µg/L to 5 mg/L nTiO2 significantly increased superoxide dismutase activity (p < .05). Glutathione content and catalase activity increased significantly after exposure to 8 µg/L nTiO2  but decreased significantly in treatments with nTiO2 concentrations ranging from 40 µg/L to 5 mg/L (p < .05). There were no significant changes in malondialdehyde contents among nTiO2 treatments and control. Overall, the present study indicated that nTiO2 revealed high toxicity to rotifers, displaying high environmental risks to aquatic ecosystems.


Assuntos
Nanopartículas Metálicas/toxicidade , Rotíferos/efeitos dos fármacos , Titânio/toxicidade , Animais , Relação Dose-Resposta a Droga , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/química , Titânio/administração & dosagem , Titânio/química , Poluentes Químicos da Água/administração & dosagem , Poluentes Químicos da Água/química , Poluentes Químicos da Água/toxicidade
12.
Artigo em Inglês | MEDLINE | ID: mdl-29614793

RESUMO

The aim of this study was to investigate how vehicle speed influences the characteristics of driver's eye movement at highway tunnel entrances during day and night. In this study, six drivers' eye movement data (from 200 m before tunnel entrance to 200 m inside tunnel entrance) under five predetermined vehicle speeds (40, 50, 60, 70 and 80 km/h) in the daytime and three predetermined vehicle speeds (40, 60 and 80 km/h) in the nighttime were recorded using the non-intrusive Dikablis Professional eye-tracking system. Pupil size, the average fixation duration time and the average number of fixation were analyzed and then the influence of the vehicle speed on these parameters was evaluated by means of IBM SPSS Statistics 20.0. The results for pupil size in daytime increased when approaching the tunnel entrance, while as for nighttime, pupil size decreased when approaching the tunnel entrance and then increased after entering the tunnel. The pupil size in daytime has a significant negative correlation with vehicle speed, while the pupil size in nighttime did not show a significant association with vehicle speed. Furthermore, the average fixation duration in daytime increased when entering the tunnel, and had a significant negative correlation with vehicle speed. Also, the average number of fixations in daytime decreased when entering the tunnel and has a significant negative correlation with vehicle speed. However, the average fixation duration and the average number of fixations in nighttime did not show any significant association with vehicle speed. Moreover, limitations and future directions of the study are discussed for the further investigation.


Assuntos
Condução de Veículo/estatística & dados numéricos , Movimentos Oculares/fisiologia , Luz , Adulto , Escuridão , Humanos , Masculino , Pessoa de Meia-Idade , Projetos Piloto
13.
Int J Ophthalmol ; 10(11): 1633-1639, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29181304

RESUMO

AIM: To investigate whether DNA vaccine encoding herpes simplex virus 1 (HSV-1) glycoprotein C (gC) and glycoprotein D (gD) will achieve better protective effect against herpes simplex keratitis (HSK) than DNA vaccine encoding gD alone. METHODS: DNA vaccine expressing gD or gC combined gD (gD.gC) were constructed and carried by chitosan nanoparticle. The expression of fusion protein gD and gC were detected in DNA/nanoparticle transfected 293T cells by Western-blot. For immunization, mice were inoculated with DNA/nanoparticle for 3 times with 2wk interval, and two weeks after the final immunization, the specific immune responses and clinical degrees of primary HSK were evaluated. RESULTS: Fusion protein gD.gC could be expressed successfully in cultured 293T cells. And, pRSC-gC.gD-IL21 DNA/chitosan nanoparticle could effectively elicit strongest humoral and cellular immune response in primary HSK mice evidenced by higher levels of specific neutralizing antibody and sIgA production, enhanced cytotoxicities of splenocytes and nature killer cells (NK), when compared with those of gD alone or mocked vaccine immunized mice. As a result, gC-based vaccine immunized mice showed least HSK disease. CONCLUSION: gC-based DNA vaccine could effectively prevent the progress of primary HSK, suggesting that this DNA vaccine could be a promising vaccine for HSK treatment in the future.

14.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 25(2): 517-521, 2017 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-28446304

RESUMO

OBJECTIVE: To investigate the serum IL-33 and sST2 levels in children with Henoch-Schonlein purpura (HSP), and explore their clinical significance. METHODS: Total 27 HSP patients and 22 healthy controls were enrolled in present study. The expressions of IL-33 and sST2 were measured by enzyme linked immunosorbent assay (ELISA). Using real-time quantitative polymerase chain reaction (RT-PCR), the mRNA expression of IL-33 and sST2 were detected in all subjects. RESULTS: The level of the IL-33 in the serum of HSP group and control group was 365.5±160.6 pg/ml and 175.9±92.8 pg/ml(P< 0.05). The level of the sST2 was increased in the serum of HSP group (1788.6±523.8 pg/ml) as compared with that in control group (1083.6±489.6 pg/ml)(P>0.05), but the ratio of sST2/IL-33 in HSP patients was much lower than that in the controls(P<0.05), IL-33 and sST2 mRNA levels were up-regulated in HSP patients by 5.47±1.97-fold(P<0.05) and 3.13±2.01-fold(P<0.05) compared with controls, but sST2/IL-33 significantly decreased in HSP patients(P<0.05). CONCLUSION: The levels of IL-33 and sST2 increase in the serum of HSP patients, but the ratio of sST2/IL-33 is much lower than that in control.


Assuntos
Interleucina-33/metabolismo , Púrpura de Schoenlein-Henoch/metabolismo , Receptores de Somatostatina/metabolismo , Autofagia , Criança , Humanos , RNA Mensageiro
15.
Cell Death Dis ; 7(9): e2377, 2016 09 29.
Artigo em Inglês | MEDLINE | ID: mdl-27685621

RESUMO

In our previous study we found that miR-612 negatively regulated stem cell-like property and tumor metastasis of hepatocellular carcinoma cells (HCC). In this study, we try to elucidate underlying mechanism of the regulation, and find that miR-612 inversely modulate the mRNA and protein level of epithelial cell adhesion molecule as well as CD133, negatively regulate the numbers and sizes of tumor spheres, directly inhibit the protein level of Sp1, and subsequently reduce transcription activity of Nanog. Of importance, the higher levels of Sp1 and Nanog in biopsies are the more unfavorable prognoses of HCC patients are found after tumor resection. Taken together, miR-612 has a suppressive role on HCC stemness via Sp1/Nanog signaling pathway.


Assuntos
Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/genética , MicroRNAs/metabolismo , Proteína Homeobox Nanog/metabolismo , Células-Tronco Neoplásicas/metabolismo , Transdução de Sinais/genética , Fator de Transcrição Sp1/metabolismo , Antígeno AC133/metabolismo , Sequência de Bases , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Imunoprecipitação da Cromatina , Molécula de Adesão da Célula Epitelial/metabolismo , Regulação Neoplásica da Expressão Gênica , Células Hep G2 , Humanos , Neoplasias Hepáticas/patologia , MicroRNAs/genética , Proteína Homeobox Nanog/genética , Regiões Promotoras Genéticas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
16.
Crit Rev Oncol Hematol ; 100: 11-5, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26830007

RESUMO

Autophagy involves the sequestration and lysosomal degradation of various cytoplasmic structures, including damaged organelles and invading microorganisms. Autophagy is not only an essential cell-intrinsic mechanism for protecting against internal and external stress conditions but is also key in the cellular response against microbes, in antigen processing for major histocompatibility complex (MHC) presentation, and in lymphocyte development, survival, and proliferation. In recent years, perturbations in autophagy have been implicated in a number of diseases, including autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and multiple sclerosis (MS). Immune thrombocytopenia (ITP) is a multifactorial disease characterized by autoimmune responses to self-platelet membrane proteins. Recently, our unpublished original data demonstrated aberrant expression of molecules in the autophagy pathway in ITP patients compared with controls, and we found a close correlation between the pathogenesis of ITP and the autophagy pathway. The potential of targeting the autophagy pathway in ITP as a novel therapeutic approach has been discussed.


Assuntos
Autofagia/fisiologia , Púrpura Trombocitopênica Idiopática/fisiopatologia , Humanos , Púrpura Trombocitopênica Idiopática/imunologia
17.
J Craniofac Surg ; 26(2): 425-9, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25668112

RESUMO

BACKGROUND: The aims of this study were to determine the best suited magnetic resonance imaging scanning plane, scanning sequence, and imaging modality for the evaluation of the temporomandibular joint (TMJ) and quantitatively assess the relationship of articular disk position to condyle position. METHODS: One hundred four TMJs in 52 symptom-free heads were examined by magnetic resonance imaging. The best scanning plane, scanning sequence, and scanning parameter were determined according to the imaging time and image quality. Bilateral symmetry of the articular disk and mandibular condyle was measured by using the automatic measurement of 3.0-T GE Excite Signa MR scanner. RESULTS: Fast spin-echo sequence, oblique sagittal imaging plane, and proton density imaging were the best suited scanning sequence, scanning planes, and imaging modality, respectively. The thicknesses of the anterior and posterior bands and for the intermediate zone were not statistically different for both sides. The posterior band of the disk was found to originate in an area adjacent to the 12-o'clock position of the condyle (± 5 degrees), whereas the anterior band of the disk originated adjacent to 1-o'clock position (28 ± 6 degrees). The anteroposterior diameter and mediolateral diameter of the condylar processes were not statistically different for both sides. The axial condylar angle between the plane of the greatest mediolateral diameter of the condylar processes and the midsagittal plane were also not statistically different for both sides. CONCLUSIONS: The magnetic resonance images can depict clearly major regional anatomic structures and position in the TMJ, which can be used in the early diagnosis for the TMJ disorder.


Assuntos
Imageamento por Ressonância Magnética/métodos , Articulação Temporomandibular/anatomia & histologia , Adolescente , Adulto , Idoso , Meato Acústico Externo/anatomia & histologia , Feminino , Humanos , Aumento da Imagem/métodos , Masculino , Côndilo Mandibular/anatomia & histologia , Pessoa de Meia-Idade , Osso Temporal/anatomia & histologia , Disco da Articulação Temporomandibular/anatomia & histologia , Fatores de Tempo , Adulto Jovem
18.
World J Pediatr ; 9(4): 346-9, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24235068

RESUMO

BACKGROUND: Acute lower respiratory tract infection (ALRI) due to adenovirus infection is a low frequency event but often causes severe outcome. This study was undertaken to uncover the clinical and epidemiological features of adenovirus infection in children. METHODS: Hospitalized children with ALRI were analyzed through continuous monitoring from 2006 to 2012. Nasopharyngeal aspirates were examined by direct immunofluorescence to detect respiratory agents including respiratory syncytial virus, adenovirus, influenza virus types A/B, parainfluenza virus types 1/2/3. Chlamydia pneumonia, Mycoplasma pneumonia and Chlamydia trachomatis were determined by real-time PCR. A retrospective analysis was made of 479 patients with positive infection of adenovirus. RESULTS: The positive detection rate of adenovirus was 0.63% in patients with ALRI. The incidence rate of adenovirus-associated acute lower respiratory tract infection peaked at the second six months of life. The morbidity was much higher in winter, spring and summer than in autumn. Patients with pneumonia accounted for 73.90% of the patients. More than one-third of the patients developed severe pneumonia, whereas no death was found. Features of severe adenovirus-associated lower respiratory tract infection included persistent high fever with serious infective symptoms, and hepatic dysfunction was one of the most common complications. Mixed infection of atypical pathogens was common (18.58%) in this study. CONCLUSIONS: Adenovirus is a critical pathogen that can cause severe respiratory infections even in immunocompetent children. Coinfection of adenovirus with atypical pathogens is common. Antibiotic treatment with azithromycin or erythromycin is necessary in patients with mixed infection of atypical pathogens.


Assuntos
Infecções por Adenoviridae/diagnóstico , Infecções Respiratórias/virologia , Fatores Etários , Bronquite/virologia , Lavagem Broncoalveolar , Broncoscopia , Pré-Escolar , Feminino , Febre/virologia , Hepatomegalia/virologia , Humanos , Incidência , Lactente , Laringite/virologia , Hepatopatias/virologia , Masculino , Pneumonia Bacteriana/complicações , Pneumonia Bacteriana/microbiologia , Pneumonia Viral/complicações , Pneumonia Viral/virologia , Sons Respiratórios , Estudos Retrospectivos , Estações do Ano , Esplenomegalia/virologia , Traqueíte/virologia
19.
Zhongguo Zhen Jiu ; 33(8): 721-4, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24195215

RESUMO

OBJECTIVE: To explore electrophysiology mechanism of acupuncture for treatment and prevention of visual deprivation effect. METHODS: Eighteen healthy 15-day Evans rats were randomly divided into a normal group, a model group and an acupuncture group, 6 rats in each one. Deprivation amblyopia model was established by monocular eyelid suture in the model group and acupuncture group. Acupuncture was applied at "Jingming" (BL 1), "Chengqi" (ST 1), "Qiuhou" (EX-HN 7) and "Cuanzhu" (BL 2) in the acupuncture group. The bilateral acupoints were selected alternately, one side for a day, and totally 14 days were required. The effect of acupuncture on visual evoked potential in different spatial frequencies was observed. METHODS: Under three different kinds of spatial frequencies of 2 X 2, 4 X 4 and 8 X 8, compared with normal group, there was obvious visual deprivation effect in the model group where P1 peak latency was delayed (P<0.01) while N1 -P1 amplitude value was decreased (P<0.01). Compared with model group, P1 peak latency was obviously ahead of time (P<0.01) while N1-P1 amplitude value was increased (P<0.01) in the acupuncture group, there was no statistical significance compared with normal group (P>0.05). Under spatial frequency of 4 X 4, N1-P1 amplitude value was maximum in the normal group and acupuncture group. With this spatial frequency the rat's eye had best resolving ability, indicating it could be the best spatial frequency for rat visual system. CONCLUSION: The visual system has obvious electrophysiology plasticity in sensitive period. Acupuncture treatment could adjust visual deprivation-induced suppression and slow of visual response in order to antagonism deprivation effect.


Assuntos
Terapia por Acupuntura , Ambliopia/terapia , Potenciais Evocados Visuais , Pontos de Acupuntura , Ambliopia/fisiopatologia , Animais , Feminino , Humanos , Masculino , Ratos , Ratos Long-Evans
20.
Zhen Ci Yan Jiu ; 38(4): 291-6, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24261299

RESUMO

OBJECTIVE: To observe the effect of acupuncture of "Zusanli" [ST 36, Ho (Sea)-acupoint] and "Zhongwan" (CV 12, front Mu-acupoint) on the expression of hypothalamic gonadotropin-releasing hormone (GnRH) mRNA and SP mRNA in stress-induced gastric ulcer (GU) rats. METHODS: Sixty male Wistar rats were randomly divided into normal control group, simple bundling group, GU model group, CV 12 group, ST 36 group and CV 12 + ST 36 (Ho- and front Mu-acupoint combination) group, with 10 cases in each group. GU model was established by binding the rat's four limbs to an animal board and to immerse it to cool water for 24 h. Manual acupuncture stimulation was applied to CV 12, bilateral ST 36 or CV 12 + ST 36 for 20 min, once daily for 2 days. The GU index was evaluated by using Guth's method (1979). GnRH mRNA and SP mRNA expression of the hypothalamus tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Compared with the normal group, the GU index values in the simple bundling group and the model group were significantly higher (P < 0.01); while in comparison with the model group, the GU index values were significantly decreased in the CV 12, ST 36 and CV 12 + ST 36 groups (P < 0.05, P < 0.01). Following stress stimulation, hypothalamic GnRH mRNA expression levels in the simple bundling group and the model group were remarkably increased (P < 0.05, P < 0.01), whereas those of hypothalamic SP mRNA expression had no apparent changes in these two groups (P > 0.05). After manual acupuncture intervention, hypothalamic GnRH mRNA expression levels in the CV 12, ST 36 and CV 12 + ST 36 groups were evidently down-regulated and SP mRNA expression levels in the ST 36 and CV 12+ ST 36 groups were remarkably upregulated in comparison with the model group (P < 0.01). No significant differences were found between the CV 12 and ST 36 groups in the GU index, among the CV 12, ST 36 and CV 12 + ST 36 groups in GnRH mRNA expression, and between the CV 12 and model groups in the expression levels of SP mRNA (P > 0.05). CONCLUSION: Acupuncture of CV 12 and ST 36 can relieve stress-induced gastric ulcer in the rat, which is closely with its effect in down-regulating hypothalamic GnRH mRNA expression.


Assuntos
Pontos de Acupuntura , Terapia por Acupuntura , Hormônio Liberador de Gonadotropina/genética , Úlcera Gástrica/terapia , Estresse Psicológico/complicações , Substância P/genética , Animais , Hormônio Liberador de Gonadotropina/metabolismo , Humanos , Hipotálamo/metabolismo , Masculino , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Fatores de Transcrição Sp , Úlcera Gástrica/etiologia , Substância P/metabolismo
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