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1.
Protoplasma ; 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404922

RESUMO

Flavonols are important active ingredients that are found in abundance in Dendrobium officinale. Research on flavonol biosynthesis currently focuses on the more ubiquitous kaempferol and quercetin, but little is known on the biosynthesis of myricetin. Notably, flavonol synthase (FLS), which is responsible for the biosynthesis of flavonols, has not yet been identified. In this study, we isolated a flavonol synthase, DoFLS1, from Dendrobium officinale. DoFLS1 harbors conserved 2-oxoglutarate-dependent dioxygenase-specific and FLS-specific motifs. DoFLS1 is a cytoplasmic protein. DoFLS1 was universally expressed in roots, stems, and leaves of juvenile and adult D. officinale plants. DoFLS1 expression was strongly correlated in juvenile and adult D. officinale plants (R2 = 0.86 and 0.98, respectively; p < 0.01) with the average of corresponding flavonol levels. Transgenic Arabidopsis thaliana expressing DoFLS1 exhibited a 1.24-fold increase in flavonol content and a 25.78% decrease in anthocyanin content compare to wild-type plants, possibly resulting from a 78.61% increase in myricetin level. Moreover, the loss of anthocyanin was attributed to decreased expression of dihydroflavonol reductase (DFR) and anthocyanidin synthase (ANS) genes in transgenic A. thaliana that expressed DoFLS1. DoFLS1 also complemented the deficiency in flavonol of the A. thaliana fls1-3 mutant, which had reduced anthocyanin but increased flavonol content relative to the fls1-3 mutant. In addition, DoFLS1 was significantly upregulated after treatment with cold, drought or salicylic acid. These findings provide genetic evidence for the involvement of DoFLS1 in the biosynthesis of flavonol and in response to abiotic stresses.

2.
Exp Gerontol ; : 111235, 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33453322

RESUMO

OBJECTIVE: Chronic inflammation is associated with major adverse cardiovascular events (MACEs), mortality, and frailty. Our aim was to add high-sensitivity C-reactive protein (hsCRP) to the frailty assessment to predict its association with prognosis of older adults with cardiovascular disease (CVD). METHODS: A comprehensive geriatric assessment was conducted at baseline in 720 in-patients aged ≥65 years with CVD. We divided the population into frailty and non-frailty groups according to the Fried phenotype, and hsCRP was further combined with frailty to stratify all patients into c-frailty and non-c-frailty groups. Predictive validity was tested using Cox proportional hazards regression model analysis and the discriminative ability was evaluated by receiver operating characteristic (ROC) curves. RESULTS: Of all the subjects enrolled, 51.0% were male and the mean age was 75.32 ±â€¯6.52 years. The all-cause death and MACE rate was 6.4% at the 1-year follow-up. Frailty and c-frailty were independent predictors of all-cause death and MACE (hazard ratio [HR]: 2.55, 95% confidence interval [CI]: 1.35-4.83, p = 0.004; HR: 3.67, 95% CI: 1.83-7.39, p < 0.001). Adding hsCRP to the frailty model resulted in a significant increase in the area under the ROC curve from 0.74 (95% CI: 0.70-0.77) to 0.77 (95% CI: 0.71-0.84) (p = 0.0132) and a net reclassification index of 7.9% (95% CI: 1.96%-12.56%, p = 0.012). CONCLUSION: Adding hsCRP to the frailty assessment is helpful to identify a subgroup of older CVD patients with a higher risk of death and MACE over a period of 1 year. TRIAL REGISTRATION: ChiCTR1800017204; date of registration: 07/18/2018. URL: http://www.chictr.org.cn/showproj.aspx?proj=28931.

3.
Carbohydr Polym ; 256: 117556, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33483059

RESUMO

Acute kidney injury (AKI) is a common and serious clinical syndrome of acute renal dysfunction in a short period. One of therapeutic interventions for AKI is to reduce ROS massively generated in the mitochondria and then ameliorate cell damage and apoptosis induced by oxidative stress. In this study, stepwise-targeting chitosan oligosaccharide, triphenyl phosphine-low molecular weight chitosan-curcumin (TPP-LMWC-CUR, TLC), was constructed for sepsis-induced AKI via removing excessive ROS in renal tubular epithelial cells. Benefiting from good water solubility and low molecular weight, TLC was rapidly and preferentially distributed in the renal tissues and then specifically internalized by tubular epithelium cells via interaction between Megalin receptor and LMWC. The intracellular TLC could further delivery CUR to mitochondria due to high buffering capacity of LMWC and delocalized positive charges of TPP. Both in vitro and in vivo pharmacodynamic results demonstrated the enhanced therapeutic effect of TLC in the treatment of AKI.

4.
J Exp Clin Cancer Res ; 40(1): 36, 2021 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-33485374

RESUMO

BACKGROUND: Aberrant expressions of long noncoding RNAs (lncRNAs) have been demonstrated to be related to the progress of HCC. The mechanisms that SNHG14 has participated in the development of HCC are obscure. METHODS: Quantitative real-time PCR (qRT-PCR) was used to measure the lncRNA, microRNA and mRNA expression level. Cell migration, invasion and proliferation ability were evaluated by transwell and CCK8 assays. The ceRNA regulatory mechanism of SNHG14 was evaluated by RNA immunoprecipitation (RIP) and dual luciferase reporter assay. Tumorigenesis mouse model was used to explore the roles of miR-876-5p in vivo. The protein levels of SSR2 were measured by western blot assay. RESULTS: In this study, we demonstrated that SNHG14 was highly expressed in HCC tissues, meanwhile, the elevated expression of SNHG14 predicted poor prognosis in patients with HCC. SNHG14 promoted proliferation and metastasis of HCC cells. We further revealed that SNHG14 functioned as a competing endogenous RNA (ceRNA) for miR-876-5p and that SSR2 was a downstream target of miR-876-5p in HCC. Transwell, CCK8 and animal experiments exhibited miR-876-5p inhibited HCC progression in vitro and in vivo. By conducting rescue experiments, we found the overexpression of SSR2 or knocking down the level of miR-876-5p could reverse the suppressive roles of SNHG14 depletion in HCC. CONCLUSION: SNHG14 promotes HCC progress by acting as a sponge of miR-876-5p to regulate the expression of SSR2 in HCC.

5.
J Neurosci ; 2021 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33468565

RESUMO

Appropriate termination of the photoresponse in image-forming photoreceptors and downstream neurons is critical for an animal to achieve high temporal resolution. Although the cellular and molecular mechanisms of termination in image-forming photoreceptors have been extensively studied in Drosophila, the underlying mechanism of termination in their downstream large monopolar cells (LMCs) remains less explored. Here, we show that synaptic acetylcholine (ACh) signaling, from both amacrine cells (ACs) and L4 neurons, facilitates the rapid repolarization of L1 and L2 neurons. Intracellular recordings in female flies show that blocking synaptic ACh output from either ACs or L4 neurons leads to slow repolarization of L1 and L2 neurons. Genetic and electrophysiological studies in both male and female flies determine that L2 neurons express ACh receptors and directly receive ACh signaling. Moreover, our results demonstrate that synaptic ACh signaling from both ACs and L4 neurons simultaneously facilitates ERG termination. Finally, visual behavior studies in both male and female flies show that synaptic ACh signaling, from either ACs or L4 neurons to L2 neurons, is essential for the optomotor response of the flies in high frequency light stimulation. Our study identifies parallel synaptic ACh signaling for repolarization of L1 and L2 neurons and demonstrates that synaptic ACh signaling facilitates L1 and L2 neurons repolarization to maintain the optomotor response of the fly upon high frequency light stimulation.SIGNIFICANCE STATEMENT:The image-forming photoreceptor downstream neurons receive multiple synaptic inputs from image-forming photoreceptors and various types of interneurons. It remains largely unknown how these synaptic inputs modulate the neural activity and function of image-forming photoreceptor downstream neurons. We show that parallel synaptic acetylcholine signaling from both amacrine cells and L4 neurons facilitate rapid repolarization of large monopolar cells in Drosophila and maintain the optomotor response of the fly upon high frequency light stimulation. This work is one of the first reports showing how parallel synaptic signaling modulate the activity of large monopolar cells and motion vision simultaneously.

6.
Thorac Cancer ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33471419

RESUMO

BACKGROUND: Adenocarcinoma of the esophagogastric junction (AEG) refers to cancer that crosses the line of the gastroesophageal junction and includes distal esophageal cancer and proximal gastric cancer. It is characterized by early metastasis and a poor prognosis and has few treatment options. Here, we report a novel potential therapeutic target, hematological and neurological expressed 1-like (HN1L), in AEG. METHODS: A total of 38 patients who underwent surgical resection of AEG at the Department of Thoracic Surgery of Shandong Provincial Hospital from September 2018 to June 2019 were enrolled into the study. We detected the expression of HN1L in AEG and adjacent nontumor tissues by IHC staining. The clinicopathological characteristics of HN1L were statistically analyzed. Then, the expression of HN1L in different cell lines was detected by RT-q PCR. Finally, AGS and HGC-27 cell lines were performed to inhibit HN1L by shRNA in order to explore its role in the development of AEG. RESULTS: Immunohistochemical staining showed that the expression of HN1L in cancer tissues was higher than that in nontumor tissue (p < 0.001). High expression of HN1L was significantly correlated with TNM stage (p = 0.013) and lymph node metastasis (p = 0.03). The expression of HN1L was upregulated in tumor cell lines compared with normal cell line. Additionally, Cell function studies demonstrated that lentivirus-mediated shRNA silencing of HN1L expression could effectively reduce the proliferation, invasion, and metastasis of tumor cell lines and promote their apoptosis (p < 0.05). CONCLUSIONS: HN1L expression might contribute to the invasion and metastasis of AEG and is a promising therapeutic target.

7.
Dev Comp Immunol ; : 104015, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33460679

RESUMO

Tumour necrosis factor receptor associated factor 3 (TRAF3) is a crucial transducing protein for linking upstream receptor signals and downstream antiviral signalling pathways. Previous studies mostly clarified the functions of TRAF3 in mammals, birds and fish, but little is known about the characterization and function of TRAF3 in amphibians. In this study, the molecular and functional identification of two TRAF3 genes, AdTRAF3A and AdTRAF3B, were investigated in the Chinese giant salamander Andrias davidianus. The complete open reading frames (ORFs) of AdTRAF3A and AdTRAF3B were 1,698 bp and 1,743 bp in length, encoding 565 and 580 amino acids, respectively. Both AdTRAF3A and AdTRAF3B deduced proteins contained a RING finger, two TRAF-type zinc fingers, a coiled-coil and a MATH domain. Phylogenetic analysis showed that the AdTRAF3 protein clustered together with other known TRAF3 proteins. Gene expression analysis showed that AdTRAF3s were broadly distributed in all examined tissues with similar distribution patterns. AdTRAF3s in the blood or spleen positively responded to Chinese giant salamander iridovirus (GSIV) and poly (I:C) induction but exhibited distinct response patterns. Silencing AdTRAF3A/B remarkably suppressed the expression of IFN signalling pathway-related genes when leukocytes were treated with DNA virus and the viral RNA analogue. Moreover, overexpression of AdTRAF3A may induce the activation of the IFN-ß promoter, and the zinc finger, coiled coil and MATH domains of AdTRAF3A were essential for IFN-ß promoter activation. However, the overexpression of AdTRAF3B significantly suppressed IFN-ß promoter activity, and its inhibitory effect was enhanced when the RING finger or MATH domain was deleted. Furthermore, AdTRAF3A rather than AdTRAF3B significantly induced NF-κB activation, implying that AdTRAF3A may function as an enhancer in both the IFN and NF-κB signalling pathways. Taken together, our results suggest that the two TRAF3 genes play different crucial regulatory roles in innate antiviral immunity in Chinese giant salamanders.

8.
Mikrochim Acta ; 188(1): 18, 2021 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404778

RESUMO

A new method based on coordination polymer nanoparticles (CPNs) derived from nucleotides and Tb3+ ions (GMP/Tb) for the selective and sensitive determination of aqueous 2,4,6-trinitrophenol (TNP) (picric acid) is established. The fluorescence of GMP/Tb nanoparticles is effectively quenched by TNP via photo-induced charge transfer (PCT), thus achieving its selectivity toward TNP over other nitroaromatic explosives. The decreased fluorescence of GMP/Tb shows a good linear relationship to the concentrations of TNP ranging from 5.0 to 40.0 µM, and the limit of detection is 26.0 nM (5.96 ppb). The proposed GMP/Tb probe also achieves satisfactory results in real samples. The obtained recoveries of this method in river water samples are in the range 93.15-106.10%. The relative standard deviation (RSD) are 0.57 to 1.01% based on three repeated determinations. This fabricated detector provides a feasible path for determination of ppb-level TNP in natural water samples, which can help humans to avoid TNP-contaminated drinking water. Graphical abstract.

9.
Arch Microbiol ; 2021 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-33386871

RESUMO

This work describes the characterization and genome annotation of Salmonella phage vB_SalM_8-19 (referred to as 8-19) isolated from sewage samples collected in a pig farm in Jilin, China. This phage was capable of infecting 60% Salmonella strains in our lab stock. The genome of phage 8-19 is composed of linear double-stranded DNA that is 52,648 bp in length with a G + C content of 46.02%; containing 74 ORFs and no tRNA genes. In October 2019, phylogenetic analyses indicated that phage 8-19 might belong to a novel cluster among the other similar phages which have not been specifically classified within some new genus in family Myoviridae. Recently, the International Committee on Taxonomy of Viruses (ICTV) defined phage 8-19 and its related phages as genus Rosemountvirus, family Myoviridae. This new genus, known as Rosemountvirus, is rarely reported in the literature.

10.
Artigo em Inglês | MEDLINE | ID: mdl-33398478

RESUMO

PURPOSE: N-myristoyltransferases 1 and 2 (NMT1 and NMT2) catalyze the addition of 14-carbon fatty acids to the N-terminus of proteins. Myristoylation regulates numerous membrane-bound signal transduction pathways important in cancer biology and the pan-NMT inhibitor PCLX-001 is approaching clinical development as a cancer therapy. The tissue distribution, relative abundances, and prognostic value of the two human NMTs remain poorly understood. METHODS: We generated and validated mutually exclusive monoclonal antibodies (mAbs) specific to human NMT1 and NMT2. These mAbs were used to perform immunohistochemical analysis of the abundance and distribution of NMT1 and NMT2 in normal breast epithelial samples and a large cohort of primary breast adenocarcinomas from the BCIRG001 clinical trial (n = 706). RESULTS: NMT1 protein was readily quantified in normal and most transformed breast epithelial tissue and was associated with higher overall histologic grade, higher Ki67, and lower hormone receptor expression. While NMT2 protein was readily detected in normal breast epithelial tissue, it was undetectable in the majority of breast cancers. Detectable NMT2 protein correlated with significantly poorer overall survival (hazard ratio 1.36; P = 0.029) and worse biological features including younger age, higher histologic grade, lower hormone receptor expression, higher Ki67, and p53 positivity. Treatment of cultured breast cancer cells with PCLX-001 reduced cell viability in vitro. Daily oral administration of PCLX-001 to immunodeficient mice bearing human MDA-MB-231 breast cancer xenografts produced significant dose-dependent tumor growth inhibition in vivo. CONCLUSIONS: These results support further evaluation of NMT immunohistochemistry for patient selection and clinical trials of NMT inhibition in breast cancer patients.

11.
Talanta ; 221: 121372, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33076049

RESUMO

Nitrogen and sulfur co-doped carbon dots (abbreviated as N,S-CDs) were obtained by two-step hydrothermal reactions using citric acid/sulfamic acid as precursors, polyethyleneimine (PEI) as passivation agent. It was found that the PEI modified CDs with a fluorescence quantum yield of up to 29.1%, showed an obviously enhanced photoluminescence (PL) compared to the initial CDs. Interestingly, when monitored at the fluorescence emission wavelength of 460 nm, the dispersed N,S-CDs solution exhibits only one excitation band peaked at 355 nm, while one aggregated N,S-CDs solution with good water solubility and excellent fluorescence stability possesses two well-separated excitation bands centered at 310 nm/397 nm. When chlorogenic acid (CGA) was added to this aggregated N,S-CDs solution, the excitation peak at 310 nm was obviously reduced due to the inner filter effect (IFE), whereas another peak at 397 nm almost remained constant. Based on the above phenomenon, a dual-excitation ratiometric fluorescent probe for CGA assay was constructed. Under the optimized conditions, the logarithm of the fluorescence intensity ratios (F397/F310) exhibited a good linear correlation with the CGA concentration over a range from 0.33 to 29.70 µg/mL with a detection limit of 0.12 µg/mL. Moreover, the proposed sensing system was applied to determine CGA content in real samples with satisfactory results. The proposed sensing platform provides a new method for the detection of CGA.

12.
Sci Total Environ ; 751: 141726, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32889464

RESUMO

Ultrafine particles (UFPs) are of concern because of their high pulmonary deposition efficiency. However, present control measures are generally targeted at fine particles (PM2.5), with little effect on UFPs. The health effects of UFPs at different PM2.5 concentrations may provide a basic for controlling UFPs but remain unclear in polluted areas. School children spend the majority of their time in the classrooms. This study investigated the different short-term effects of indoor UFPs on school children in Beijing, China when indoor PM2.5 concentrations exceeded or satisfied the recently published Chinese standard for indoor PM2.5. Cardiopulmonary functions of 48 school children, of whom 46 completed, were measured three times. Indoor PM2.5 and UFPs were monitored in classrooms on weekdays. Measurements were separated into two groups according to the abovementioned standard. Mixed-effect models were used to explore the health effects of the air pollutants. Generally, UFP-associated effects on children's cardiopulmonary function persisted even at relatively low PM2.5 concentrations, especially on heart rate variability indices. The risks associated with high PM2.5 concentrations are well-known, but the effects of UFPs on children's cardiopulmonary function deserve more attention even when PM2.5 has been controlled. UFP control and standard setting should therefore be considered.


Assuntos
Poluentes Atmosféricos , Poluição do Ar em Ambientes Fechados , Poluentes Atmosféricos/efeitos adversos , Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Pequim , Criança , China , Monitoramento Ambiental , Humanos , Tamanho da Partícula , Material Particulado/efeitos adversos , Material Particulado/análise
13.
Bioresour Technol ; 319: 124127, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32971331

RESUMO

The effects of two nitrogen fertilizer synergists (urease inhibitor, UI; nitrification inhibitor, NI) on NH3 and N2O emissions and the successions of the amoA and nirS genes during composting were assessed. Results showed that the UI and UI + NI treatments reduced NH3 emissions by 26.3% and 24.3%, respectively, and N2O emissions were reduced by 63.9% for UI + NI treatment but were not reduced by UI. The addition of UI and NI significantly reduced the abundance of the nirS gene during thermophilic stage, while significantly increased that of the amoA gene during maturation stage. Crenarchaeota was the principal nitrifying archaeal phylum and was significantly affected by pH. Proteobacteria was the main denitrifying bacterial phylum, whose relative abundance was higher for UI + NI treatment than the other treatments. PICRUSt analysis showed that the addition of UI and NI inhibited enzymatic activity related to N transformation during thermophilic stage while enriching enzymatic activity during maturation phase.


Assuntos
Compostagem , Agricultura , Desnitrificação , Fertilizantes , Gases , Nitrogênio , Óxido Nitroso/análise , Solo
14.
J Sci Food Agric ; 101(2): 624-630, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32687643

RESUMO

BACKGROUND: As labeling thresholds and low level presence thresholds of genetically modified (GM) components are implemented in more and more countries and regions, the demands for accurate quantification are increasing rapidly. At the same time, digital polymerase chain reaction (PCR) showed considerable benefits compared with former real-time fluorescence PCR in GM component quantification. RESULTS: A universal quantification method using duplex digital PCR was established for detection of transgenic soybean event DAS-68416-4. The absolute limits of quantification (LOQs) of DAS-68416-4 event-specific gene and lectin reference gene were 0.61 copies µL-1 and 4.6 copies µL-1 respectively in droplet digital PCR, while 0.522 copies µL-1 and 5.192 copies µL-1 in chip digital PCR. The relative LOQs of DAS-68416-4 percentage content was 0.1% in both two digital PCR systems. CONCLUSION: Gene copy ratio is the universal means of expression internationally used in transgenic component contents. Digital polymerase chain reaction (PCR) executes absolute quantification on specific genes, thus is considered to be suitable for detection of transgenic component contents. It was proved in this research on transgenic soybean event DAS-68416-4. Results indicated perfect satisfaction for transgenic component quantification needs in various technical performances of duplex digital PCR including repeatability, quantitative linear relationship and relative limits of quantification. © 2020 Society of Chemical Industry.

15.
Front Neurol ; 11: 523292, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33304303

RESUMO

Objective: To investigate the predictive value of prognosis of primary GBM patients using TMT on three-dimensional (3D) MR images of the brain. Methods: Data of 130 patients with primary GBM from the TCGA-GBM database were analyzed retrospectively. TMT was measured on the axial plane by multi-planar reformation (MPR) of T1WI MR images perpendicular to the long axis of the temporal muscle at the level of the orbital roof. The axial MR plane was oriented parallel to the anterior commissure-posterior commissure line. Student's t-test or Mann-Whitney U-test was utilized to determine whether there were significant differences in the TMT and OS between male and female patients. The Pearson correlation analysis was adopted to evaluate the correlation between the age at GBM diagnosis and TMT. All patients were divided into two groups based on their median TMT, and the Kaplan-Meier curve was used to calculate the OS curve. The association between TMT and OS of GBM patients, as well as the multivariate analysis of TMT and other clinical factors affecting the survival time, was evaluated with Cox regression model. Results: TMT was a risk factor for the prognosis of GBM with its hazard ratio (HR) of 0.802 (95% CI 0.698-0.922; P = 0.002; Cox regression model). Grouped by median TMT, the group with above-median TMT demonstrated a significant increase in survival time (15.6 months) compared with the one with below-median TMT (11.2 months) (P < 0.001; log-rank test). In the multivariate survival analysis using a Cox regression model, TMT (HR 0.863; 95% CI 0.748-0.996; P = 0.044), age at the diagnosis of GBM (HR 1.042; 95% CI 1.024-1.060; P < 0.001), and concurrent chemoradiotherapy (HR 0.510; 95% CI 0.336-0.775; P = 0.002) were significantly associated with survival time. Conclusion: TMT as an independent predictor is sensitive to the survival prognosis of primary GBM patients, which has potential to predict the survival time.

16.
PeerJ ; 8: e10353, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33304652

RESUMO

Understanding animals' migration, distribution and activity patterns is vital for the development of effective conservation action plans; however, such data for many species are lacking. In this study, we used camera trapping to document the spatial and temporal activity patterns of golden takins (Budorcas taxicolor bedfordi) in Changqing National Nature Reserve in the Qinling mountains, China, from April 2014 to October 2017. Our study obtained 3,323 independent detections (from a total of 12,351 detections) during a total camera trapping effort of 93,606 effective camera trap days at 573 sites. Results showed that: (1) the golden takin's utilization distributions showed seasonal variation, with larger utilization distributions during spring and autumn compared to summer and winter; (2) the species was recorded at the highest elevations in July, and lowest elevations in December, with the species moving to higher-elevations in summer, lower-elevations in spring and autumn; (3) during all four seasons, golden takins showed bimodal activity peaks at dawn and dusk, with activity intensity higher in the second peak than the first, and overall low levels of activity recorded from 20:00-06:00; and (4) there were two annual activity peaks, the first being in April and the second in November, with camera capture rate during these two months higher than in other months, and activity levels in spring and autumn higher than in summer and winter. This study is the first application of camera traps to assess the spatial and temporal activity patterns of golden takins at a population level. Our findings suggest that the proposed national park should be designed to include golden takin habitat and that ongoing consistent monitoring efforts will be crucial to mitigating novel and ongoing threats to the species.

17.
PLoS One ; 15(12): e0243884, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33301523

RESUMO

PURPOSES: Since a considerable number of health care workers (HCWs) were sent to Wuhan to aid COVID-19 control during the epidemic, non-frontline HCWs who stayed in local hospitals had to work overload to provide daily health care services for other health issues, which makes them more vulnerable to experience fatigue. Self-efficacy is suggested as a protective factor for fatigue. Nonetheless, less is known regarding the underlying mechanisms. This research aimed to explore the prevalence of fatigue among non-frontline HCWs during the pandemic, investigate the mediating effect of posttraumatic stress disorder (PTSD) symptoms and moderating effect of negative coping in the association between self-efficacy and fatigue. METHODS: General Self-Efficacy Scale, PTSD Checklist-Civilian Version, Simplified Coping Style Questionnaire and 14-item Fatigue Scale were administrated to 527 non-frontline HCWs from Anhui Province, China. The mediating effect was examined by Mackinnon's four-step procedure, while Hayes PROCESS macro was used to test the moderated mediation model. RESULTS: The prevalence of fatigue among non-frontline HCWs was 56.7%. The effect of self-efficacy on fatigue was partially mediated by PTSD symptoms (ab = -0.146, SE = 0.030, 95% CI = [-0.207, -0.095]). Additionally, negative coping moderated both the direct effect of self-efficacy on fatigue (ß = -0.158, P<0.001) and the mediating effect of PTSD symptoms (ß = 0.077, P = 0.008). When the standard score of negative coping increased to 1.49 and over, the direct association between self-efficacy and fatigue became insignificant. Likewise, the effect of self-efficacy on PTSD symptoms had no statistical significance when the standard score of negative coping was -1.40 and lower. CONCLUSIONS: More than half non-frontline HCWs suffered from fatigue during COVID-19. For those who tend to use negative coping, it might be crucial to design programs combining the enhancement of self-efficacy, preventions for PTSD symptoms and interventions for fatigue.

18.
Medicine (Baltimore) ; 99(49): e23123, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33285686

RESUMO

BACKGROUND: Bisphosphonates are commonly used to treat spontaneous osteonecrosis of the knee (SONK), while there are no relevant systematic review or meta-analysis designed to evaluate the effects of bisphosphonates on SONK. METHODS: We will identify relevant randomized controlled trials from the PubMed, EMBASE, CINAHL and China National Knowledge Infrastructure, up to March 20, 2020. Data that meets the inclusion criteria will be extracted and analyzed using RevMan V.5.3 software. Two reviewers will assess quality of the included studies by using the Cochrane Collaboration risk of bias tool. Egger test and Begg test will be used to evaluate publication bias. And Grading of Recommendations Assessment, Development and Evaluation will be employed to assess the quality of evidence. RESULTS: In this study, we will analyze the effect of bisphosphonates on pain intensity, physical function, biochemical including alkaline phosphatase, N-terminal propeptide of type I procollagen, and C-terminal type I collagen telopeptide, radiological outcome (evaluated by using Magnetic resonance imaging) and ratio of secondary surgery for patients with SONK. CONCLUSION: Our findings will provide evidence for the effectiveness and potential treatment prescriptions of bisphosphonates acupuncture for patients affected by SONK.

19.
Adv Mater ; : e2006093, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33274802

RESUMO

Fabrication of soft piezoelectric nanomaterials is essential for the development of wearable and implantable biomedical devices. However, a big challenge in this soft functional material development is to achieve a high piezoelectric property with long-term stability in a biological environment. Here, a one-step strategy for fabricating core/shell poly(vinylidene difluoride) (PVDF)/dopamine (DA) nanofibers (NFs) with a very high ß-phase content and self-aligned polarization is reported. The self-assembled core/shell structure is believed essential for the formation and alignment of ß-phase PVDF, where strong intermolecular interaction between the NH2 groups on DA and the CF2 groups on PVDF is responsible for aligning the PVDF chains and promoting ß-phase nucleation. The as-received PVDF/DA NFs exhibit significantly enhanced piezoelectric performance and excellent stability and biocompatibility. An all-fiber-based soft sensor is fabricated and tested on human skin and in vivo in mice. The devices show a high sensitivity and accuracy for detecting weak physiological mechanical stimulation from diaphragm motions and blood pulsation. This sensing capability offers great diagnostic potential for the early assessment and prevention of cardiovascular diseases and respiratory disorders.

20.
Sci Rep ; 10(1): 20900, 2020 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-33262425

RESUMO

One of the challenges with urgent evaluation of patients with acute respiratory distress syndrome (ARDS) in the emergency room (ER) is distinguishing between cardiac vs infectious etiologies for their pulmonary findings. We conducted a retrospective study with the collected data of 171 ER patients. ER patient classification for cardiac and infection causes was evaluated with clinical data and chest X-ray image data. We show that a deep-learning model trained with an external image data set can be used to extract image features and improve the classification accuracy of a data set that does not contain enough image data to train a deep-learning model. An analysis of clinical feature importance was performed to identify the most important clinical features for ER patient classification. The current model is publicly available with an interface at the web link: http://nbttranslationalresearch.org/ .

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