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1.
Int Immunopharmacol ; 80: 106179, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31972422

RESUMO

Network pharmacology is a novel approach that uses bioinformatics to predict and identify multiple drug targets and interactions in disease. Here, we used network pharmacology to investigate the mechanism by which triptolide acts in rheumatoid arthritis (RA). We first searched public databases for genes and proteins known to be associated with RA, as well as those predicted to be targets of triptolide, and then used Ingenuity Pathway Analysis (IPA) to identify enriched gene pathways and networks. Networks and pathways that overlapped between RA-associated proteins and triptolide target proteins were then used to predict candidate protein targets of triptolide in RA. The following proteins were found to occur in both RA-associated networks and triptolide target networks: CD274, RELA, MCL1, MAPK8, CXCL8, STAT1, STAT3, c-JUN, JNK, c-Fos, NF-κB, and TNF-α. Docking studies suggested that triptolide can fit in the binding pocket of the six top candidate triptolide target proteins (CD274, RELA, MCL1, MAPK8, CXCL8 and STAT1). The overlapping pathways were activation of Th1 and Th2 cells, macrophages, fibroblasts and endothelial cells in RA, while the overlapping networks were involved in cellular movement, hematological system development and function, immune cell trafficking, cell-to-cell signaling and interaction, inflammatory response, cellular function and maintenance, and cell death and survival. These results show that network pharmacology can be used to generate hypotheses about how triptolide exerts therapeutic effects in RA. Network pharmacology may be a useful method for characterizing multi-target drugs in complex diseases.

2.
Int Immunopharmacol ; 74: 105725, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31276975

RESUMO

Network pharmacology uses bioinformatics to broaden our understanding of drug actions and thereby to advance drug discovery. Here we apply network pharmacology to generate testable hypotheses about the multi-target mechanism of celastrol against rheumatoid arthritis. We reconstructed drug-target pathways and networks to predict the likely protein targets of celastrol and the main interactions between those targets and the drug. Then we validated our predictions of four candidate targets (IKK-ß, JNK, COX-2, MEK1) by performing docking studies with celastrol. The results suggest that celastrol acts against rheumatoid arthritis by regulating the function of several signaling proteins, including MMP-9, COX-2, c-Myc, TGF-ß, c-JUN, JAK-1, JAK-3, IKK-ß, SYK, MMP-3, JNK and MEK1, which regulate the functions of Th1 and Th2 cells, macrophages, fibroblasts and endothelial cells in rheumatoid arthritis. Celastrol is predicted to affect networks involved mainly in cancer, connective tissue disorders, organismal injury and abnormalities, tissue development, cell death and survival. This network pharmacology strategy may be useful for discovery of multi-target drugs against complex diseases.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Biologia Computacional , Doenças do Tecido Conjuntivo/metabolismo , Descoberta de Drogas/métodos , Macrófagos/imunologia , Neoplasias/metabolismo , Farmacologia , Células Th1/imunologia , Células Th2/imunologia , Triterpenos/farmacologia , Morte Celular , Doenças do Tecido Conjuntivo/tratamento farmacológico , Humanos , Simulação de Acoplamento Molecular , Neoplasias/tratamento farmacológico , Organogênese , Transdução de Sinais/efeitos dos fármacos , Triterpenos/uso terapêutico
3.
Medicine (Baltimore) ; 98(21): e15777, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31124968

RESUMO

The present study sought to assess the feasibility and effectiveness of double stent insertion as a means of managing combined malignant airway and superior vena cava (SVC) obstruction (CMASO).From July 2010 to January 2018, twelve consecutive patients with CMASO were treated by double stent insertion (airway and SVC stents) in our centers. We assessed data pertaining to both technical and clinical success rates, as well as to long-term patient outcomes.The use of double stents (12 airway stents and 19 SVC stents) was technically successful in all study subjects, with a 0 to 92 days period between the 2 stent insertions (mean 27.8 days). Patients did not show evidence of any procedure-related complications. Mean patient Hugh-Jones grades improved from 4.4 ±â€Š0.5 before inserting the airway stent down to 1.2 ±â€Š0.4 following this insertion (P < .001). Mean SVC pressure was reduced from 17.5 ±â€Š2.8 mm Hg before the stent insertion down to 6.7 ±â€Š1.4 mm Hg following this insertion (P < .001). Fifty-six days after insertion, a single patient experienced re-obstruction of their SVC stent. All patients died within the follow-up period, with a median survival time of 113 days for these 12 patients.This double stent insertion protocol is both effective and safe as a means of offering palliative care to those with CMASO.


Assuntos
Obstrução das Vias Respiratórias/cirurgia , Neoplasias Pulmonares/cirurgia , Implantação de Prótese/instrumentação , Stents , Síndrome da Veia Cava Superior/cirurgia , Idoso , Obstrução das Vias Respiratórias/etiologia , Estudos de Viabilidade , Feminino , Humanos , Neoplasias Pulmonares/complicações , Masculino , Pessoa de Meia-Idade , Cuidados Paliativos/métodos , Implantação de Prótese/métodos , Estudos Retrospectivos , Síndrome da Veia Cava Superior/complicações , Resultado do Tratamento
4.
ACS Chem Neurosci ; 10(5): 2397-2407, 2019 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-30720268

RESUMO

In this study, we designed and synthesized a series of deoxyvasicinone-donepezil hybrids and determined whether they could be used as novel multitarget inhibitors for Alzheimer's disease. In vitro studies showed that most of the hybrids demonstrated moderate to potent inhibition of hAChE, BACE1, and Aß1-42 aggregation. In particular, the hybrids 10a, 10d, 11a, and 11j exhibited excellent inhibitory activities against hAChE (IC50 = 56.14, 5.91, 3.29, and 8.65 nM, respectively), BACE1 (IC50 = 0.834, 0.167, 0.129, and 0.085 µM, respectively), and Aß1-42 aggregation (IC50 = 13.26, 19.43, 9.26, and 5.41 µM, respectively). In addition, 10a and 11a exhibited very low cytotoxicity and showed remarkable neuroprotective activity against Aß1-42-induced damage in SH-SY5Y cells.

6.
Eur J Med Chem ; 140: 118-127, 2017 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-28923380

RESUMO

A series of multitarget ligands was designed by introducing several structurally diverse aminoacetamide groups at position 6 of the deoxyvasicinone group, with the aim of obtaining novel multifunctional anti-Alzheimer's disease agents using deoxyvasicinone as the substrate. In vitro studies showed that almost all of the derivatives were potent inhibitors of human recombinant acetylcholinesterase (hAChE) and human serum butyrylcholinesterase (hBChE), with IC50 values in the low nanomolar range, and exhibited moderate to high inhibition of Aß1-42 self-aggregation. In particular, compounds 12h, 12n, and 12q showed promising inhibitory activity for hAChE, with IC50 values of 5.31 ± 2.8, 4.09 ± 0.23, and 7.61 ± 0.53 nM, respectively. Compounds 12h and 12q also exhibited the greatest ability to inhibit hBChE, with IC50 values of 4.35 ± 0.32 and 2.35 ± 0.14 nM, respectively. Moreover, enzyme kinetics confirmed that compound 12q caused a mixed type of AChE inhibition, by binding to both the active sites (PAS and CAS) of AChE. Remarkably, compound 12q also demonstrated the highest potential inhibitory activity for Aß1-42 self-aggregation (63.9 ± 4.9%, 10 µM), and it was also an excellent metal chelator.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Inibidores da Colinesterase/farmacologia , Desenho de Fármacos , Quinazolinas/farmacologia , Acetilcolinesterase/metabolismo , Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/antagonistas & inibidores , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/síntese química , Inibidores da Colinesterase/química , Relação Dose-Resposta a Droga , Humanos , Cinética , Ligantes , Estrutura Molecular , Fragmentos de Peptídeos/antagonistas & inibidores , Agregados Proteicos/efeitos dos fármacos , Quinazolinas/síntese química , Quinazolinas/química , Relação Estrutura-Atividade
7.
Oncol Lett ; 14(1): 180-184, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28693151

RESUMO

The application value of small intestine decompression combined with oral feeding in the middle and late period of malignant small bowel obstruction was examined. A total of 22 patients with advanced malignant small bowel obstruction were included in the present study. An ileus tube was inserted via the nose under fluoroscopy into the obstructed small intestine of each patient. At the same time, the insertion depth the of the catheter was adjusted. When the catheter was blocked, small bowel selective angiography was performed to determine the location and cause of the obstruction and the extent of the obstruction, and to determine the length of the small intestine in the site of obstruction, and to select the variety and tolerance of enteral nutrition. We observed the decompression tube flow and ease of intestinal obstruction. In total, 20 patients were treated with oral enteral nutrition after abdominal distension, and 22 cases were treated by the nose to observe the drainage and the relief of intestinal obstruction. The distal end of the catheter was placed in a predetermined position. The symptoms of intestinal obstruction were relieved 1-4 days after decompression. The 22 patients with selective angiography of the small intestine showed positive X-ray signs: 18 patients with oral enteral nutrition therapy had improved the nutritional situation 2 weeks later. In 12 cases, where there was anal defecation exhaust, 2 had transient removal of intestinal obstruction catheter. In conclusion, this comprehensive treatment based on small intestine decompression combined with enteral nutrition is expected to become a new therapeutic approach and method for the treatment of patients with advanced tumor small bowel obstruction.

8.
Oncol Lett ; 13(1): 137-140, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28123533

RESUMO

Colorectal cancer, which includes colon and rectal cancer, is a common digestive tract tumor. Although surgery is the primary form of treatment, there are a number of drawbacks, including patients experiencing considerable pain and high cost. The present study was undertaken to examine the clinical value of transanal ileal tube placement under X-ray monitoring. Thirty-six cases of left colon obstruction presenting to our hospital between July 2011 and February 2014, underwent transanal ileal tube placement using a single-curve catheter guided by a guidewire under X-ray monitoring. An ileal tube was successfully inserted into 32 patients. Clinical symptoms were alleviated effectively within 48 h. Indwelling catheter decompression time was 4-9 days with an average of 5.61 days. In two cases, the colon guidewire perforated into the abdominal cavity. Repeated exploration resistance of the guidewire and catheter indicated stenosis at this position owing to obstruction. In conclusion, transanal placement of the ileal tube through X-ray monitoring is capable of effectively alleviating the symptoms of ileus. Thus, this constitutes a safe, effective, and economical method that is acceptable to patients.

9.
Water Sci Technol ; 74(5): 1127-35, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27642832

RESUMO

In this work, pyridinium-functionalized silica nanoparticles adsorbent (PC/SiO2/Fe3O4) was synthesized for phosphate removal from aqueous solutions. The removal efficiency of phosphate on the PC/SiO2/Fe3O4 was carried out and investigated under various conditions such as pH, contact temperature and initial concentration. The results showed that the adsorption equilibrium could be reached within 10 min, which fitted a Langmuir isotherm model, with maximum adsorption capacity of 94.16 mg/g, and the kinetic data were fitted well by pseudo-second-order and intra-particle diffusion models. Phosphate loaded on the adsorbents could be easily desorbed with 0.2 mol/L of NaOH, and the adsorbents showed good reusability. The adsorption capacity was still around 50 mg/g after 10 times of reuse. All the results demonstrated that this pyridinium-functionalized mesoporous material could be used for the phosphate removal from aqueous solution and it was easy to collect due to its magnetic properties.


Assuntos
Nanopartículas/química , Fosfatos/química , Dióxido de Silício/química , Poluentes Químicos da Água/química , Adsorção , Difusão , Cinética , Magnetismo , Temperatura , Purificação da Água/métodos
10.
Carbohydr Polym ; 151: 348-357, 2016 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-27474576

RESUMO

In this research, extraction optimization, preliminary characterization and immunological activities in vitro of polysaccharides from Elaeagnus angustifolia L. pulp were investigated. A response surface methodology (RSM) with a Box-Behnken design (BBD) was used to optimize the extraction process. The maximum EAP yield was 9.82±0.38%, which is in good agreement with the predicted value (9.93±0.24%). Two homogeneous polysaccharides, EAP-1a and EAP-1b with molecular weights of 8.70kDa and 4.39kDa respectively, were prepared by DEAE-52 cellulose and Sephadex G-100 columns and characterized by HPLC, HPGPC, and FT-IR. Three polysaccharides (EAP, EAP-1a and EAP-1b) could stimulate macrophages to release NO and enhance phagocytic activities of RAW 264.7 cells in dose-dependent manner. Moreover, there was no significant difference between crude EAP group (400µg/mL) and positive control group (LPS) in effects on macrophages. The results implied that EAP had the potential to be developed as natural medicines or health foods.


Assuntos
Cromatografia/métodos , Elaeagnaceae/química , Macrófagos/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Animais , Dextranos , Frutas/química , Camundongos , Polissacarídeos/imunologia , Células RAW 264.7
11.
Bioorg Med Chem Lett ; 26(16): 4015-9, 2016 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-27397495

RESUMO

A series of N(9)-substituted harmine derivatives were synthesized and evaluated for their anticancer activity on a panel of cancer cell lines, their apoptosis induction and their cell cycle effects. The results showed that N(9)-substituted harmine derivatives had anticancer effects. In particular, N(9)-haloalkyl derivatives 9a-9c and N(9)-acyl harmine derivatives 11c and 11d, with IC50 values less than 1µM, were more potent than doxorubicin against A-549 and/or MCF-7 cell lines. Moreover, structure-activity relationships (SARs) indicated that introducing a haloalkyl or benzenesulfonyl group in the N(9)-position of harmine could significantly increase the anticancer activity. The most active compound (11d) caused cell cycle arrest in the G2/M phase, and induced cell apoptosis in a dose-dependent manner.


Assuntos
Antineoplásicos/síntese química , Harmina/química , Células A549 , Antineoplásicos/química , Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Harmina/síntese química , Harmina/toxicidade , Humanos , Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos , Células MCF-7 , Relação Estrutura-Atividade
12.
Int J Mol Sci ; 17(7)2016 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-27347944

RESUMO

A response surface and Box-Behnken design approach was applied to augment polysaccharide extraction from the residue of Rhizoma gastrodiae. Statistical analysis revealed that the linear and quadratic terms for three variables during extraction exhibited obvious effects on extraction yield. The optimum conditions were determined to be a liquid-to-solid ratio of 54 mL/g, an extraction temperature of 74 °C, an extraction time of 66 min, and three extractions. These conditions resulted in a maximum Rhizoma gastrodiae polysaccharide (RGP) extraction yield of 6.11% ± 0.13%. Two homogeneous polysaccharides (RGP-1a and RGP-1b) were obtained using DEAE cellulose-52 and Sephadex G-100 columns. The preliminary characterization of RGP-1a and RGP-1b was performed using HPLC-RID, HPGPC, and FTIR. Tests of the immunological activity in vitro showed that the two polysaccharides could significantly stimulate macrophages to release NO and enhance phagocytosis in a dose-dependent manner. In particular, RGP-1b (200 µg/mL) and LPS (2 µg/mL) had almost the same influence on the NO production and phagocytic activity of RAW 264.7 macrophages (p > 0.05). All the data obtained indicate that RGP-1a and RGP-1b have the potential to be developed as a health food.


Assuntos
Fatores Imunológicos/química , Orchidaceae/química , Extratos Vegetais/química , Polissacarídeos/química , Animais , Linhagem Celular , Fatores Imunológicos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Óxido Nítrico/metabolismo , Fagocitose , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Rizoma/química
13.
Bioorg Med Chem Lett ; 26(2): 580-583, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26681509

RESUMO

An improved synthetic route of canthin-6-one was accomplished. To further enhance the antibacterial potency and improve water solubility, a series of 3-N-alkylated and 3-N-benzylated canthin-6-ones were designed and synthesized, and their in vitro antibacterial activities were evaluated. A clear structure-activity relationship with peak minimal inhibitory concentration (MIC) values of 0.98 (µg·mL(-)(1)) was investigated. Particularly, compounds 6i-r and 6t were found to be the most potent compounds with minimal inhibitory concentration (MIC) values lower than 1.95 (µg·mL(-)(1)) against Staphylococcus aureus.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Carbolinas/química , Carbolinas/farmacologia , Alcaloides Indólicos/química , Alcaloides Indólicos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Alquilação , Antibacterianos/síntese química , Carbolinas/síntese química , Humanos , Alcaloides Indólicos/síntese química , Testes de Sensibilidade Microbiana , Infecções Estafilocócicas/tratamento farmacológico , Relação Estrutura-Atividade
14.
Surg Laparosc Endosc Percutan Tech ; 25(6): 500-4, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26492458

RESUMO

PURPOSE: To investigate the long-term outcome of palliative stent insertion for acute malignant colorectal obstruction. METHODS: From May 2009 to February 2015, consecutive patients with acute malignant colorectal obstruction underwent palliative stent insertion in our center. Technical success, clinical success, and long-term outcomes were analyzed retrospectively. RESULTS: A total of 45 patients with acute malignant colorectal obstruction underwent palliative stent insertion. Technical success was achieved in 42 of 45 patients. Clinical success was achieved in 41 of 42 patients. During a follow-up of 5 days to 25 months (mean, 6.9±4.5 mo), the cumulative 6- and 12-month patency rates were 88.6% and 72.7%, respectively. The cumulative 6- and 12-month survival rates were 60.1% and 14.3%, respectively. The independent predictor of prolonging survival was subsequent chemotherapy after stenting (P=0.017). CONCLUSION: Palliative colorectal stent insertion can provide a good long-term outcome in patients with malignant colorectal obstruction.


Assuntos
Adenocarcinoma/patologia , Neoplasias Colorretais/patologia , Obstrução Intestinal/cirurgia , Cuidados Paliativos , Stents , Adenocarcinoma/mortalidade , Adenocarcinoma/cirurgia , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/cirurgia , Feminino , Humanos , Obstrução Intestinal/etiologia , Obstrução Intestinal/mortalidade , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Taxa de Sobrevida , Resultado do Tratamento
15.
Gastroenterol Res Pract ; 2015: 121060, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26451141

RESUMO

Objective. To investigate the serum level of CA-125 and its corresponding clinical significance in Chinese patients with primary BCS. Methods. Serum CA-125 was measured in 243 patients with primary BCS receiving interventional treatment in the participating hospitals and in 120 healthy volunteers. The correlation between serum CA-125 levels and ascites volume, liver function, and prognosis was analyzed. Results. Serum CA-125 was significantly elevated in BCS patients compared to healthy volunteers (P < 0.001). Higher levels of CA-125 were found in BCS patients with abnormal hepatic function and low serum albumin levels and in patients with high volume of ascites compared to patients without these abnormalities. Serum CA-125 levels significantly correlated with ascites volume, serum level of alanine aminotransferase, aspartate aminotransferase, albumin, and Rotterdam BCS scores. The follow-up study indicated that the survival rate and asymptomatic survival rate after interventional treatment were lower in BCS patients with serum CA-125 > 175 U/mL (P < 0.05). Conclusion. Serum CA-125 was significantly higher in patients with primary BCS and had a positive correlation with the volume of ascites, severity of liver damage, and poor prognosis. Thus the serum CA-125 levels may be used to estimate the severity and prognosis of BCS in Chinese patients.

16.
J Gastrointest Surg ; 19(12): 2243-8, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26354721

RESUMO

PURPOSE: The aim of this study is to investigate the feasibility, safety, and effectiveness of placing the decompression tube as a bridge to surgery for acute malignant left-sided colonic obstruction. METHODS: From January 2009 to August 2014, consecutive patients with acute malignant left-side colonic obstruction underwent placement of the decompression tube as a bridge to surgery in our center. The technical and clinical success of placing the decompression tube was evaluated. Clinical success was defined as relief of obstructive symptoms within 48 h after placing the decompression tube. Elective tumor resection was performed 7-9 days after colonic decompression. The types of surgery, primary anastomosis rate, and follow-up findings were analyzed. RESULTS: Twenty patients with acute malignant left-side colonic obstruction underwent placement of the decompression tube as a bridge to surgery. Placement of decompression tube was technically successful in all patients. No procedure-related complication occurred. Clinical success was achieved in 19 patients. Elective tumor resection and primary anastomosis were successfully performed in all 19 patients. The postoperative complications included wound infection (n = 2) and anastomotic stenosis (n = 1). CONCLUSION: Decompression tube can serve as an easy, safe, and effective bridge to subsequent surgery for patients with acute malignant left-sided colonic obstruction.


Assuntos
Doenças do Colo/cirurgia , Descompressão Cirúrgica/instrumentação , Obstrução Intestinal/cirurgia , Intubação/instrumentação , Adulto , Idoso , Colectomia , Doenças do Colo/etiologia , Doenças do Colo/mortalidade , Procedimentos Cirúrgicos Eletivos , Feminino , Humanos , Obstrução Intestinal/etiologia , Obstrução Intestinal/mortalidade , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
17.
Spectrochim Acta A Mol Biomol Spectrosc ; 136 Pt B: 900-5, 2015 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-25459614

RESUMO

A novel sensing system based on the ß-carboline core has been designed and synthesized for Hg(2+) detection in water. We have demonstrated that a straight forward methodology can provide rapid, sensitive and selective recognition (cross-contamination experiments) for Hg(2+) over a wide pH range. The vivid fluorescence change from blue to colorless can be clearly discriminated by the naked eye. Furthermore, there is a good negative correlation between the fluorescent intensity and the concentration of Hg(2+) in the range 1.0×10(-6) M-7.0×10(-6) M. ß-Carboline as a fluorophore synthesized via this route also provides a new strategy for the design of novel fluorescence probes and fluorochromes.


Assuntos
Acetais/química , Carbolinas/síntese química , Corantes Fluorescentes/síntese química , Mercúrio/análise , Água/química , Carbolinas/química , Corantes Fluorescentes/química , Concentração de Íons de Hidrogênio , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta
18.
Abdom Imaging ; 40(1): 76-84, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25063237

RESUMO

BACKGROUND: Magnetic resonance imaging (MRI) is able to diagnose Budd-Chiari syndrome (BCS) by distinguishing differential imaging features of acute and chronic forms of the disease. However, the characteristic imaging differences are still not clear as previous data were mostly obtained from scattered small samples instead of large comparative study. AIM: To investigate MRI manifestations of acute and chronic (BCS), and to evaluate the value of MRI for diagnosis of acute and chronic BCS. METHODS AND RESULTS: We retrospectively compared MRI results of 24 patients with acute and 82 patients with chronic BCS using Mann-Whitney U test for ascites volume, and Fisher's exact test for intrahepatic venous collaterals and extra-hepatic venous collaterals. In the acute group, MRI findings suggested thrombosis in hepatic vein (HV) in all acute patients and additional inferior vena cava (IVC) thrombosis in 5 patients. In the chronic BCS group, 6 and 15 patients showed solitary obstruction either in the IVC or HV, respectively, while 61 patients showed combined IVC and HV obstruction. More patients with acute BCS presented with ascites accompanied with high signals on T2WI from intravenous obstructive lesions. Further, the average maximal spleen diameter in patients with acute BCS, and the ratio of patients with acute BCS developing intrahepatic venous collaterals and extra-hepatic venous collaterals were also lower compared with chronic BCS. All these differences were statistically significant. CONCLUSION: MRI indicates direct and indirect features of BCS, and therefore enables accurate diagnosis of acute and chronic BCS.


Assuntos
Síndrome de Budd-Chiari/diagnóstico , Imagem por Ressonância Magnética , Doença Aguda , Adulto , Doença Crônica , Meios de Contraste , Diagnóstico Diferencial , Feminino , Gadolínio DTPA , Veias Hepáticas/patologia , Humanos , Aumento da Imagem , Fígado/irrigação sanguínea , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Estudos Retrospectivos , Veia Cava Inferior/patologia , Adulto Jovem
19.
Int J Mol Sci ; 15(7): 11446-55, 2014 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-24972139

RESUMO

In this study, two polysaccharides (Elaeagnus angustifolia L. polysaccharide-1 (PEA-1) and PEA-2) were prepared from Elaeagnus angustifolia L. Then, the preliminary structure and antioxidant activities of all the samples were investigated. The results showed that the average molecular weights for PEA-1 and PEA-2 were 9113 and 5020 Da, respectively. And, PEA-1 was mainly composed of rhamnose, xylose, mannose, glucose, and galactose, respectively. The components of PEA-2 were rhamnose, mannose, glucose, and galactose, respectively. Moreover, the Antioxidant assays demonstrated that PEA-1 possessed of strong free radicals scavenging activity and hydroxyl radicals scavenging activities, suggesting that PEA-1 could potentially be used as natural antioxidant.


Assuntos
Antioxidantes/química , Elaeagnaceae/química , Extratos Vegetais/química , Polissacarídeos/química , Galactose/análise , Glucose/análise , Manose/análise , Oxirredução , Ramnose/análise , Xilose/análise
20.
Zhonghua Gan Zang Bing Za Zhi ; 21(11): 850-4, 2013 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-24331696

RESUMO

OBJECTIVE: To prospectively study clinical features and etiology in patients with incident Budd-Chiari syndrome (BCS) in China. METHODS: Taking consecutive case series of patients with incident BCS as who were diagnosed in our hospital, enrolled from September 2010 to January 2012 as the object of research, and the follow-up was lasting until June 2012. Taking records for all patients' symptoms, signs, laboratory findings, radiology findings, treatment, interventional treatment survival and symptom-free period. RESULTS: There are total 149 incident cases of BCS. In which, the median age was 46 years old (range 10 to 82); 61.7% of them were male patients, 38.3% were female patients; 85.9% of them were chronic, the other patients (14.1%) were diagnosed during acute or subacute periods; the median duration of symptoms before diagnosis was 96 months (range 1 day to 360 months). In terms of causes, 30.9% of the patients caused by hepatic venous block, 5.4% of them resulted from inferior vena cava block, and the rest 63.8% were suffered from combined hepatic venous and inferior vena cava block. 80.5% patients have at least one etiological factor, Furthermore, the most common cause was the web (61.1%), only 4.8% have myeloproliferative diseases (JAK2 V617F mutation), and none Factor V Leiden mutation cases was found. 144 patients were treated by percutaneous transluminal angioplasty, the technical success rate was 95.1%, and took oral anticoagulation therapy for 12 months. At 18 months, The survival rate and the symptom-free survival rate after percutaneous transluminal angioplasty were 97.8% and respectively. CONCLUSION: Web is the most prevalent etiological factor for BCS in China. It is different in western countries; the common reasons of BCS are risk factors related to thrombosis, such as myeloproliferative disease and Factor V Leiden mutation, etc., which are seldom found in Chinese BCS patients. In China, most chronic BCS patients were treated with percutaneous transluminal angioplasty and have excellent clinical outcome.


Assuntos
Síndrome de Budd-Chiari/diagnóstico , Síndrome de Budd-Chiari/etiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
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