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1.
J Neurochem ; 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33025573

RESUMO

Molecular abnormalities within the Glucocorticoid Receptor (GR) stress signaling pathway involved in dysfunction of mitochondria and confer vulnerability to stress related psychiatric disorders. Bcl-2 associated athanogene (Bag-1) is a target for the actions of mood stabilizers. Bag-1 interacts with GR, thereby regulating glucocorticoid function. In this study, we investigate the potential role of Bag-1 in regulating GR translocation into mitochondria. Corticosterone (CORT) treatment significantly enhanced Bag-1/GR complex formation and GR mitochondrial translocation in cultured rat cortical neurons after treatment for 30 minutes and 24 hours. By contrast, after stimulation with CORT for 3 days, localization of the Bag-1/GR complex and mitochondrial GR were reduced. Similar results were obtained in mice, in which administrated CORT in drinking water for 21 days significantly impaired the GR levels in the mitochondria, while Bag-1 overexpression rescued this reduction. Furthermore, chronic CORT exposure led to anhedonia-like and depression-like behaviors in the sucrose-consumption test and forced swimming test, and these behaviors were rescued by Bag-1 overexpression. These results suggest that Bag-1 mediates GR trafficking to mitochondria and regulates affective resilience in response to a CORT increase and provide potential insight into the mechanisms by which Bag-1 and GR could contribute to the physiology and pathogenesis of psychiatric disorders in response to the change of stress hormone.

2.
Sci Rep ; 10(1): 17457, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33060739

RESUMO

The aim of this study was to investigate the effect of high fat diet and excessive compressive mechanical force on temporomandibular joint. In vivo, a mouse model of temporomandibular joint compressive loading device was used. A high fat diet mouse model and a combined mouse model intraperitoneally treated with or without simvastatin were used in the study. The pathological changes of mandibular condylar cartilage were assessed by Safranin-O staining. The IL-1ß, MMP-3, leptin expression changes in the cartilage were detected by immunohistochemistry. In vitro, the mandibular condylar chondrocytes were treated with or without L-1ß and simvastatin. The mRNA expression level of matrix MMPs and leptin were assessed. Both excessive compressive mechanical force and high fat diet induced obesity caused TMJ osteoarthritis-like changes and increased expression of IL-1ß, MMP-3, and leptin. These pathological changes were much more serious when the two interventions were exerted together, while simvastatin could obviously alleviate these changes. The mRNA expression of MMP-3, MMP-13, and leptin increased in the IL-1ß treated chondrocytes treated with IL-1ß, and decreased with simvastatin treatment. The development of temporomandibular joint pathological changes could be caused by the excessive compressive mechanical force and high fat diet induced obesity.

3.
Pharm Biol ; 58(1): 1044-1054, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33027592

RESUMO

CONTEXT: Aaptamine is a potent ocean-derived non-traditional drug candidate against human cancers. However, the underlying molecular mechanisms governing aaptamine-mediated repression of lung cancer cells remain largely undefined. OBJECTIVE: To examine the inhibitory effect of aaptamine on proliferation and progression of non-small cell lung carcinoma (NSCLC) and dissect the potential mechanisms involved in its anticancer functions. MATERIALS AND METHODS: In vitro assays of cell proliferation, cell cycle analysis, clonal formation, apoptosis and migration were performed to examine the inhibitory effects of aaptamine (8, 16 and 32 µg/mL) on NSCLC cells. The expression levels of proteins were analysed using western blotting analysis when cells were treated with a single drug or a combination treatment for 48 h. RESULTS: Aaptamine significantly inhibited A549 and H1299 cells proliferation with IC50 values of 13.91 and 10.47 µg/mL. At the concentrations of 16 and 32 µg/mL, aaptamine significantly reduced capacities in clonogenicity, enhanced cellular apoptosis and decreased the motile and invasive cellular phenotype. In addition, aaptamine arrested cell cycle at G1 phase via selectively abating cell cycle regulation drivers (CDK2/4 and Cyclin D1/E). Western blotting results showed that aaptamine attenuated the protein expression of MMP-7, MMP-9 and upregulated the expression of cleaved-PARP and cleaved-caspase 3. Moreover, aaptamine inhibited PI3K/AKT/GSK3ß signalling cascades through specifically degrading the phosphorylated AKT and GSK3ß. DISCUSSION AND CONCLUSIONS: Aaptamine retarded the proliferation and invasion of NSCLC cells by selectively targeting the pathway PI3K/AKT/GSK3ß suggesting it as a potential chemotherapeutic agent for repressing tumorigenesis and progression of NSCLC in humans.

4.
Biomed Pharmacother ; 132: 110805, 2020 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-33045614

RESUMO

Skin expansion is widely used in reconstructive surgery to obtain supplemental and optimal skin. Enhancing the regenerative capacity of expanded skin is therefore of great interest. Hair follicle bulge-derived stem cells (HFBSCs) located in hair follicle bulges are closely associated with skin; HFBSC transplantation could promote cutaneous wound repair. However, the effects of transplanted HFBSCs on skin regeneration during expansion remain unclear. The aim of the study was to reveal the potential effects of transplanted HFBSCs in the expanded skin and explore its mechanism. Our results showed higher skin area, tissue weight, epidermal thickness, dermal thickness, proliferating cell count, collagen content, microcirculatory blood flow, blood vessels, and lower retraction ratios were observed in HFBSC-injected rats compared to uninjected controls. Moreover, the transplanted HFBSCs directly contributed to tissue regeneration by differentiating into vascular endothelial cells, epidermal cells, and the outer root sheath cells of hair follicle. Higher expression of EGF, VEGF, bFGF, and TGF-ß were observed in HFBSC-injected rats. Our research demonstrated the transplanted HFBSCs could promote skin regeneration by differentiating into various types of skin related cells and by up-regulating the expression of growth factors. Our results could form a basis for the development of novel strategies to enhance regeneration in expanded skin by using HFBSCs.

5.
J Integr Neurosci ; 19(3): 449-458, 2020 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-33070524

RESUMO

We first explore the features of GluK2 endocytosis during kainate excitotoxicity and then explore the role of Ca2+ in the regulation of GluK2 endocytosis. The roles of Ca2+ were examined by treating cells with Ca2+ inhibitors or chelators. Surface biotinylation was used to examine the surface localization of GluK2. Immunoprecipitation followed by immunoblotting was used to identify the interaction of GluK2 with the endocytosis regulator protein-interacting with C kinase 1 and dynamin. Dynamin phosphorylation was examined by immunoblotting with the corresponding antibodies. Our results show that GluK2 internalization is blocked by inhibitors of clathrin-independent endocytosis and relies on intracellular Ca2+/calcineurin signaling. Protein-interacting with C kinase 1-GluK2 interaction is regulated by Ca2+/calcineurin signaling. Dynamin participates in the regulation of GluK2 surface localization. Also, calcineurin activation is related to dynamin function during kainate excitotoxicity. In conclusion, GluK2 receptor endocytosis is probably a clathrin-independent and dynamin-dependent process regulated by the peak Ca2+ transient. This work indicates the roles of the Ca2+ network in the regulation of GluK2 endocytosis during kainate excitotoxicity.

6.
Oncol Rep ; 44(4): 1747-1757, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32945501

RESUMO

The main active compound of Garcinia hanburyi (referred to as gamboge) is gambogic acid (GA), which has long been a Chinese herbal medicine for treating several types of cancer. However, the potential therapeutic role and mechanisms of GA in T­cell acute lymphoblastic leukemia (T­ALL) remain unclear. In the present study, the effects of GA on proliferation, cell cycle, apoptosis, and autophagy in T­ALL cell lines were investigated. The possible mechanisms underlying GA activity were also examined. The results showed that GA inhibited proliferation, induced apoptosis, and activated autophagy in T­ALL cell lines (Jurkat and Molt­4 cells). Findings confirmed that GA has an antileukemia effect against peripheral blood lymphocyte cells in patients with ALL. GA inhibited phospho­GSK3ß S9 (p­GSK3ß S9) protein levels to inactivate Wnt signaling and suppress ß­catenin protein levels. In addition, the inhibitory effect of GA on T­ALL was reversed by overexpression of ß­catenin. Thus, GA can inhibit the growth and survival of T­ALL cells. GA also had antileukemic activity, at least in part, through the downregulation of the Wnt/ß­catenin signaling pathway.

8.
J Sci Food Agric ; 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32869886

RESUMO

BACKGROUND: The effect of multiple co-pigments on the color intensification of mulberry anthocyanins (ACs) using spectroscopic techniques in combination with a molecular docking study was studied. The hyperchromicity of ACs co-pigmented with chlorogenic acid (CH) and quercetin (Q) blends was measured and their color stability in liquid and encapsulated particle models was evaluated. RESULTS: Multiple co-pigments exhibited higher hyperchromicity, pKH -values, and heat-stability than their individual counterparts. Surflex-docking findings confirmed that stronger binding occurred between multiple ligands and AC than single ones due to their extra -OH, -COOH groups, and delocalization systems. The binding was allowed by increased H-bonding, van der Waals forces, and π-π sites by the extra groups of the multiple co-pigments with AC in aqueous juice and whey particle-based models. CONCLUSION: This is the first report of the ternary mixture of phenolic acid-flavonol-anthocyanin which could be used as promising food red-colorants. © 2020 Society of Chemical Industry.

9.
Ecotoxicol Environ Saf ; 207: 111251, 2020 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-32905935

RESUMO

A mildly one-pot method is developed for the synthesis of quasi-networked Au1.5Pt1Co1 ternary alloy nanoparticles (TANPs) at room temperature through the co-reduction of AuCl4-, PtCl6- and Co2+ with hydrazine hydrate. Characterizations of XRD, XPS, HRTEM, EDS and SAED successfully reveal the crystal structure, composition, valence and morphology of Au1.5Pt1Co1 TANPs, respectively. The glassy carbon electrode (GCE) modified by Au1.5Pt1Co1 TANPs with good dispersion and multi-density surface defects occupies the optimal electrochemical active surface area (ECSA). After the coated poly-L-cysteine (P-L-Cys) film on the Au1.5Pt1Co1/GCE surface, the morphology, element mapping and surface roughness of the P-L-Cys/Au1.5Pt1Co1/GCE are investigated via FESEM and AFM to verify continuous electrode modification processes. The electrochemical behaviors of the composite electrode for hydroquinone (HQ) are evaluated by cyclic voltammetry (CV) with interfacial properties of adsorption and diffusion. Differential pulse voltammetry (DPV) for HQ electrochemical sensing at 0.10 V (vs. SCE) exhibits two linear response ranges from 0.1 to 30 and 30-200 µM, respectively. A low detection limit (S/N = 3) of 0.045 µM is obtained with a sensitivity of 4.247 µA µM-1·cm-2. The resulting P-L-Cys/Au1.5Pt1Co1/GCE also presents ascendant selectivity, repeatability, reproducibility and stability. In addition, the established method is applied to the assessment of the HQ level in real water samples (mineral water, tap water and lake water) with the satisfactory results of spiked recoveries. The sensor may become a promising tool for the trace analysis of the electroactive substance in food or environmental samples.

10.
Math Biosci Eng ; 17(4): 3252-3259, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32987528

RESUMO

To investigate the significance of lymph node micrometastasis in T1N0 early gastric cancer. Lymph node micrometastasis may be a key mechanism in the recurrent T1N0 EGC patients after surgical treatment. It's unknow whether it is safe to leave the lymph nodes with micrometastasis untreated after ESD. A total of 106 T1N0 EGC patients were enrolled in this study. Immunohistochemical technique with CAM5.2 was employed to detect lymph node micrometastasis, and Immunohistochemical with D2-40 was used to detect the lymphatic vessels. Prognostic significance of lymph node micrometastasis and the relationship of lymph nodes micrometastasis with Clinicopathological features were analyzed. Twenty-two of the 106 T1N0 EGC cases were detected with lymph nodes micrometastasis, with the detection rate of 20.8%. The median survival time of the group with positive lymph nodes micrometastasis was lower than that of the group with negative micrometastasis, 48 vs 60 months. The incidence of lymph nodes micrometastasis in submucosal T1N0 EGC was 23.9%, while no micrometastasis was found in the mucosal T1N0 EGC. Of all the 30 cases according with the expanded ESD indications, six patients were found with lymph nodes micrometastasis. The occurrence of lymph node micrometastasis was common in T1N0 EGC. The cases with positive lymph nodes micrometastasis showed a lower median survival time than those with negative micrometastasis. lymph nodes micrometastasis incidence was higher in the submucosal ECG than in the mucosal ECG. lymph nodes micrometastasis was also found in the cases according to the expanded ESD indications.

11.
Neuroscience ; 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32896599

RESUMO

Cerebral small vessel disease (CSVD) is a common disease among elderly individuals and recognized as a major cause of vascular cognitive impairment. Recent studies demonstrated that CSVD is a disconnection syndrome. However, due to the covert neurological symptoms and subtle changes in clinical performance, the connection alterations during the stage of preclinical cognitive impairment (PCI) and mild cognitive impairment (MCI) are usually neglected and still largely unknown. Using diffusion tensor imaging (DTI), we investigated the early structural network changes in PCI and MCI patients. The PCI group demonstrated well preserved rich-club organization, less nodal strength loss, and disruption of connections centered in the feeder and local connections. Nevertheless, the MCI group manifested a disruption in the rich-club organization, a worse nodal strength loss especially in hub nodes, and an overall disturbance in rich-club, feeder and local connections. Moreover, in all CSVD patients, the strength of the rich-club, feeder and local connections was significantly correlated with multiple cognitive scores, especially in attention, executive, and memory domains; while in MCI patients, only the strength of the rich-club connections was significantly correlated with cognition. Furthermore, based on the network-based statistic analysis, we also identified distinct network component disruption pattern between the PCI group and the MCI group, validating the results described above. These results suggest a disruption pattern from peripheral to central connections with the change of cognitive status, shedding light on the early identification and the underlying disruption of CSVD.

12.
Environ Health ; 19(1): 88, 2020 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-32807188

RESUMO

BACKGROUND: Evidence on the association between exposure to perfluoroalkyl and polyfluoroalkyl substances (PFASs) and blood glucose concentrations in pregnant women is inconsistent. This study aimed to examine the association between PFAS exposure and the concentrations of fasting plasma glucose (FPG) and one-hour plasma glucose (1 h-PG) after a 50-g oral glucose tolerance test in pregnant women. METHODS: The study was based on the Shanghai-Minhang Birth Cohort, in which 1292 pregnant women were recruited. Among them, 981 women provided blood samples (at 12-16 gestational weeks) for PFAS measurement. FPG data collected from 856 women at 12-20 GW and 1 h-PG data collected from 705 women at 20-28 GW were obtained through medical records from the routine prenatal care system. High FPG or 1 h-PG was defined as ≥90th percentile of FPG or 1 h-PG. The analysis of eight PFASs was conducted in this study: perfluorohexane sulfonate (PFHxS), perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUdA), perfluorododecanoic acid (PFDoA), and perfluorotridecanoic acid (PFTrDA). The odds ratios (ORs) and associated 95% confidence intervals (CIs) were estimated to determine the associations of each PFAS compound with high FPG and 1 h-PG from a logistic regression model. RESULTS: After adjustment for potential confounders, most PFASs were positively associated with high 1 h-PG concentrations. The OR for high 1 h-PG concentrations was 1.87 (95% CI: 1.15-3.05) with a one log unit increase of PFOS; similar associations were observed for PFNA (OR: 2.15, 95% CI: 1.24-3.74), PFDA (OR: 1.61, 95% CI: 1.10-2.44), PFUdA (OR: 1.71, 95% CI: 1.12-2.62), and PFDoA (OR: 1.34, 95% CI: 1.00-1.81). When the PFAS concentrations were categorized into three groups by tertiles, the highest tertiles of PFOS, PFOA, PFNA, PFDA, PFDoA, and PFTrDA had a statistically significant increase in the risk of high 1 h-PG concentrations compared with the lowest tertiles. No statistically significant association was observed between PFAS exposure and high FPG. CONCLUSION: PFAS exposure was associated with an increased risk of high 1 h-PG among pregnant women, but no such association was observed for FPG.

13.
ACS Appl Mater Interfaces ; 12(33): 37470-37476, 2020 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-32814410

RESUMO

Hollow nanoparticles have received an enormous amount of attention in the field of nanomedicine. Herein, water-soluble hollow bimetallic complex nanoparticles, holmium(III)/iridium(III) bimetallic complex nanoparticles (Ir-Ho HNPs), were fabricated via a coordination assembly. Owing to the special metal-to-ligand charge transfer (MLCT) and the heavy-atom effect of Ir(III) in an iridium complex, Ir-Ho HNPs exhibited an intense phosphorescence and the generation of singlet oxygen (1O2). With the long electron relaxation time and high magnetic moment of Ho(III), Ir-Ho HNPs presented a high longitudinal relaxivity (r2) value (160.0 mM-1 s-1at 7.0 T). Their unique hollow structure resulted in their strong and stable ultrasound signal in an aqueous solution. As a proof of concept, Ir-Ho HNPs have been developed for the phosphorescence imaging and photodynamic therapy for living cells, ultrasound imaging, and high-field magnetic resonance imaging in vivo. Our work opened up an avenue for novel application of an iridium complex in cancer theranostics.

14.
Sci Total Environ ; 749: 141520, 2020 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-32827818

RESUMO

Comfortable outdoor environment benefits the health of citizens and reduces energy consumption and pollution. This study discusses different outdoor thermal sensation and comfort evaluation methods in severe cold area. The database was from a year-long outdoor thermal comfort survey conducted in Harbin, China. Thermal sensation evaluation was developed using meteorological parameters and three popular thermal comfort indices including Standard Effective Temperature (SET*), Physiologically Equivalent Temperature (PET) and Universal Thermal Climate Index (UTCI). Thermal comfort prediction was developed by the three thermal comfort indices and acceptability. Original thermal sensation scales of SET* and PET were less applicable to predict thermal sensation vote (TSV). Calibrated scales of the three indices were obtained based on linear regression results and probit analysis. The accuracies of calibrated scales of thermal sensation were all below 32.8%. The comfortable thermal sensation range in severe cold area varied from "slightly cool" to "hot". This calibrated range improved accuracies of thermal comfort predicting by around 20%. The unacceptability appropriate to define comfortable range was 9% on the cold thermal sensation side and 26% on the hot side. Adaptation and local exposure also acted on thermal sensation and comfort apart from factors included in thermal comfort indices. Our results provide practical thermal sensation and thermal comfort scales for severe cold area. The discussions indicate the significance of considering adaptation and local exposure for further improving thermal sensation and comfort predicting.

15.
Opt Lett ; 45(15): 4144-4147, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32735244

RESUMO

We study the relationship between the input phase delays and the output mode orders when using a pixel-array structure fed by multiple single-mode waveguides for tunable orbital-angular-momentum (OAM) beam generation. As an emitter of a free-space OAM beam, the designed structure introduces a transformation function that shapes and coherently combines multiple (e.g., four) equal-amplitude inputs, with the kth input carrying a phase delay of (k-1)Δφ. The simulation results show that (1) the generated OAM order ℓ is dependent on the relative phase delay Δφ; (2) the transformation function can be tailored by engineering the structure to support different tunable ranges (e.g., l={-1},{-1,+1},{-1,0,+1}, or {-2,-1,+1,+2}); and (3) multiple independent coaxial OAM beams can be generated by simultaneously feeding the structure with multiple independent beams, such that each beam has its own Δφ value for the four inputs. Moreover, there is a trade-off between the tunable range and the mode purity, bandwidth, and crosstalk, such that the increase of the tunable range leads to (a) decreased mode purity (from 91% to 75% for l=-1), (b) decreased 3 dB bandwidth of emission efficiency (from 285 nm for l={-1} to 122 nm for l={-2,-1,+1,+2}), and (c) increased crosstalk within the C-band (from -23.7 to -13.2dB when the tunable range increases from 2 to 4).

16.
Adv Physiol Educ ; 44(3): 453-458, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32795125

RESUMO

Traditional oral examination (TOE) is criticized for the shortage of objectivity, standardization, and reliability. These perceived limitations can be mitigated by the introduction of structured oral examination (SOE). There is little evidence of the implementation of SOE in physiology laboratory courses. The purpose of this study was to investigate the effect of SOE in laboratory-based learning sessions. Second-year medical students (n = 114) attended a 16-wk physiology laboratory course. They were initially assessed by TOE in the middle of the academic term. The students' perspectives on this assessment were measured by a modified three-point Likert-type scale questionnaire. Following this, faculty members prepared topics for SOE; nine topics were included from each laboratory course. The correct answers and scoring criteria were discussed among the faculty before the SOE event. One week after the last laboratory course, SOE was carried out for each student. As with the TOE process, student feedback was collected via a modified three-point Likert-type scale questionnaire. The mean laboratory homework score from the first four and last four laboratory courses was also calculated. Paper exams were also conducted after TOE and SOE. The results show that SOE is more acceptable to students than TOE. Significant differences (P < 0.05) were observed in terms of uniformity of questions asked, syllabus coverage, and anxiety levels. In addition, SOE improved students' performance in the laboratory course explored here. We contend that SOE shows promise as an effective assessment tool in laboratory-based physiology learning sessions.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32860205

RESUMO

PURPOSE: To screen novel mutations in LHCGR responsible for empty follicle syndrome and explore the pathological mechanism of mutations. METHODS: Four affected individuals diagnosed with infertility-associated anovulation or oligo-ovulation from three independent families were recruited. Sanger sequencing was used to identify the LHCGR mutations in affected individuals. Western blot was performed to evaluate the effects of mutations on LHCGR protein levels. Immunofluorescence was done to explore the effects of mutations on LHCGR subcellular localization. The ATP levels were measured to infer the functional effects of the mutations on LHCGR. RESULTS: In the present study, three novel biallelic mutations in LHCGR were identified in four affected individuals from three independent families with empty follicle syndrome or oligo-ovulation. All biallelic mutations were inherited from the proband of their parents. The western blot showed that the identified mutations decreased LHCGR protein level and altered the glycosylation pattern. The immunofluorescence showed an ectopic subcellular localization of LHCGR in cultured HeLa cells. Besides, the mutations in LHCGR also reduced the cellular ATP consumption. CONCLUSION: These findings confirm previous studies and expand the mutational spectrum of LHCGR, which will provide genetic diagnostic marker for patients with empty follicle syndrome.

19.
Int J Mol Sci ; 21(16)2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32824240

RESUMO

Increased platelet activation and apoptosis are characteristic of diabetic (DM) platelets, where a Parkin-dependent mitophagy serves a major endogenous protective role. We now demonstrate that Parkin is highly expressed in both healthy platelets and diabetic platelets, compared to other mitochondria-enriched tissues such as the heart, muscle, brain, and liver. Abundance of Parkin in a small, short-lived anucleate cell suggest significance in various key processes. Through proteomics we identified 127 Parkin-interacting proteins in DM platelets and compared them to healthy controls. We assessed the 11 highest covered proteins by individual IPs and confirmed seven proteins that interacted with Parkin; VCP/p97, LAMP1, HADHA, FREMT3, PDIA, ILK, and 14-3-3. Upon further STRING analysis using GO and KEGG, interactions were divided into two broad groups: targeting platelet activation through (1) actions on mitochondria and (2) actions on integrin signaling. Parkin plays an important role in mitochondrial protection through mitophagy (VCP/p97), recruiting phagophores, and targeting lysosomes (with LAMP1). Mitochondrial ß-oxidation may also be regulated by the Parkin/HADHA interaction. Parkin may regulate platelet aggregation and activation through integrin signaling through interactions with proteins like FREMT3, PDIA, ILK, and 14-3-3. Thus, platelet Parkin may regulate the protection (mitophagy) and stress response (platelet activation) in DM platelets. This study identified new potential therapeutic targets for platelet mitochondrial dysfunction and hyperactivation in diabetes mellitus.

20.
Artigo em Chinês | MEDLINE | ID: mdl-32791609

RESUMO

Objective:To develop a questionnaire with good reliability and validity that can comprehensively evaluate the influencing factors of life quality of patients with sudden deafness. Method:Based on the Green model and literature review, group discussions, semi-structured interviews and Delphi expert inquiries, an initial questionnaire was developed. From May 2018 to April 2019, 170 patients with sudden deafness in otolaryngology head and neck surgery in 2 comprehensive tertiary level A hospitals in Shanxi Province were selected for investigation by convenient sampling method. Through the critical ratio method and correlation analysis method, the data of each item are sorted and analyzed. The structural validity of the questionnaire was tested by exploratory factor analysis. The content validity of the questionnaire was evaluated by calculating the content validity index through expert inquiry. The reliability of the questionnaire was determined by calculating Cronbach's α coefficient and retesting the questionnaire, and finally a formal questionnaire was formed. Result:The questionnaire on influencing factors of life quality for patients with sudden deafness based on Green model includes 5 dimensions and 31 items including knowledge factors, attitude factors, behavior factors, contributing factors and reinforcement factors. The results of project analysis showed that the critical ratio of each item was 8.09-44.77(P<0.05). Except items 8, 11 and 13, the pearson correlation coefficient between the other items and the total score is>0.3. The KMO value of the questionnaire was 0.844, and the 2 value of Bartlett's spherical test was 3218, P<0.001. Using the principal component analysis method, five common factors were extracted, named as knowledge factor, attitude factor, behavior factor, contributing factor and reinforcing factor, respectively, the cumulative variance contribution rate was 60.905%. The content validity index of the questionnaire level was 0.955, the content validity index at item level is 0.786-0.981; Cronbach's α coefficient at the questionnaire level was 0.875, Cronbach's α coefficient of each dimension is 0.780-0.912; The retest reliability of the questionnaire level was 0.88, the retest reliability of each dimension is 0.810-0.913. Conclusion:The questionnaire on influencing factors of quality of life for patients with sudden deafness based on Green model has good reliability and validity, and can be used as a tool for evaluating influencing factors of quality of life for patients with sudden deafness.


Assuntos
Perda Auditiva Súbita , Qualidade de Vida , Análise Fatorial , Humanos , Reprodutibilidade dos Testes , Inquéritos e Questionários
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