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1.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 38(1): 56-64, 2021 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-33899428

RESUMO

Medical magnetic nanoparticles are nano-medical materials with superparamagnetism, which can be collected in the tumor tissue through blood circulation, and magnetic particle imaging technology can be used to visualize the concentration of magnetic nanoparticles in the living body to achieve the purpose of tumor imaging. Based on the nonlinear magnetization characteristics of magnetic particles and the frequency characteristics of their magnetization, a differential detection method for the third harmonic of magnetic particle detection signals is proposed. It was modeled and analyzed, to study the nonlinear magnetization response characteristics of magnetic particles under alternating field, and the spectral characteristics of magnetic particle signals. At the same time, the relationship between each harmonic and the amount of medical magnetic nanoparticle samples was studied. On this basis, a signal detection experimental system was built to analyze the spectral characteristics and power spectral density of the detected signal, and to study the relationship between the signal and the excitation frequency. The signal detection experiment was carried out by the above method. The experimental results showed that under the alternating excitation field, the medical magnetic nanoparticles would generate a spike signal higher than the background sensing signal, and the magnetic particle signal existed in the odd harmonics of the detected signal spectrum. And the spectral energy was concentrated at the third harmonic, that is, the third harmonic magnetic particle signal detection that meets the medical detection requirement could be realized. In addition, the relationship between each harmonic and the particle sample volume had a positive growth relationship, and the detected medical magnetic nanoparticle sample volume could be determined according to the relationship. At the same time, the selection of the excitation frequency was limited by the sensitivity of the system, and the detection peak of the third harmonic of the detection signal was reached at the excitation frequency of 1 kHz. It provides theoretical and technical support for the detection of medical magnetic nanoparticle imaging signals in magnetic particle imaging research.


Assuntos
Nanopartículas de Magnetita , Magnetismo
2.
Med Phys ; 2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33890681

RESUMO

PURPOSE: The low-dose computed tomography (CT) imaging can reduce the damage caused by x-ray radiation to the human body. However, low-dose CT images have a different degree of artifacts than conventional CT images, and their resolution is lower than that of conventional CT images, which can affect disease diagnosis by clinicians. Therefore, methods for noise-level reduction and resolution improvement in low-dose CT images have inevitably become a research hotspot in the field of low-dose CT imaging. METHODS: In this paper, residual attention modules (RAMs) are incorporated into the residual encoder-decoder convolutional neural network (RED-CNN) and generative adversarial network with Wasserstein distance (WGAN) to learn features that are beneficial to improving the performances of denoising networks, and developed models are denoted as RED-CNN-RAM and WGAN-RAM, respectively. In detail, RAM is composed of a multi-scale convolution module and an attention module built on the residual network architecture, where the attention module consists of a channel attention module and a spatial attention module. The residual network architecture solves the problem of network degradation with increased network depth. The function of the attention module is to learn which features are beneficial to reduce the noise level of low-dose CT images to reduce the loss of detail in the final denoising images, which is also the key point of the proposed algorithms. RESULTS: To develop a robust network for low-dose CT image denoising, multidose-level torso phantom images provided by a cooperating equipment vendor are used to train the network, which can improve the network's adaptability to clinical application. In addition, a clinical dataset is used to test the network's migration capabilities and clinical applicability. The experimental results demonstrate that these proposed networks can effectively remove noise and artifacts from multidose CT scans. Subjective and objective analyses of multiple groups of comparison experiments show that the proposed networks achieve good noise suppression performance while preserving the image texture details. CONCLUSION: In this study, two deep learning network models are developed using multidose-level CT images acquired from a commercial spiral CT scanner. The two network models can reduce and even remove streaking artifacts, and noise from low-dose CT images confirms the effectiveness of the proposed algorithms.

3.
Lancet Digit Health ; 3(4): e250-e259, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33766289

RESUMO

BACKGROUND: Strategies for integrating artificial intelligence (AI) into thyroid nodule management require additional development and testing. We developed a deep-learning AI model (ThyNet) to differentiate between malignant tumours and benign thyroid nodules and aimed to investigate how ThyNet could help radiologists improve diagnostic performance and avoid unnecessary fine needle aspiration. METHODS: ThyNet was developed and trained on 18 049 images of 8339 patients (training set) from two hospitals (the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China, and Sun Yat-sen University Cancer Center, Guangzhou, China) and tested on 4305 images of 2775 patients (total test set) from seven hospitals (the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China; the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; the Guangzhou Army General Hospital, Guangzhou, China; the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; the First Affiliated Hospital of Sun Yat-sen University; Sun Yat-sen University Cancer Center; and the First Affiliated Hospital of Guangxi Medical University, Nanning, China) in three stages. All nodules in the training and total test set were pathologically confirmed. The diagnostic performance of ThyNet was first compared with 12 radiologists (test set A); a ThyNet-assisted strategy, in which ThyNet assisted diagnoses made by radiologists, was developed to improve diagnostic performance of radiologists using images (test set B); the ThyNet assisted strategy was then tested in a real-world clinical setting (using images and videos; test set C). In a simulated scenario, the number of unnecessary fine needle aspirations avoided by ThyNet-assisted strategy was calculated. FINDINGS: The area under the receiver operating characteristic curve (AUROC) for accurate diagnosis of ThyNet (0·922 [95% CI 0·910-0·934]) was significantly higher than that of the radiologists (0·839 [0·834-0·844]; p<0·0001). Furthermore, ThyNet-assisted strategy improved the pooled AUROC of the radiologists from 0·837 (0·832-0·842) when diagnosing without ThyNet to 0·875 (0·871-0·880; p<0·0001) with ThyNet for reviewing images, and from 0·862 (0·851-0·872) to 0·873 (0·863-0·883; p<0·0001) in the clinical test, which used images and videos. In the simulated scenario, the number of fine needle aspirations decreased from 61·9% to 35·2% using the ThyNet-assisted strategy, while missed malignancy decreased from 18·9% to 17·0%. INTERPRETATION: The ThyNet-assisted strategy can significantly improve the diagnostic performance of radiologists and help reduce unnecessary fine needle aspirations for thyroid nodules. FUNDING: National Natural Science Foundation of China and Guangzhou Science and Technology Project.


Assuntos
Inteligência Artificial , Tomada de Decisões Assistida por Computador , Aprendizado Profundo , Diagnóstico por Computador/métodos , Nódulo da Glândula Tireoide/diagnóstico , Área Sob a Curva , China/epidemiologia , Humanos , Valor Preditivo dos Testes , Curva ROC , Sensibilidade e Especificidade
4.
Acta Otolaryngol ; 141(5): 490-494, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33784954

RESUMO

BACKGROUND: Combining acoustic and electrical stimulation has been successfully used in patients with low-frequency residual hearing. Electrode insertion trauma, such as electrode translocation could result in loss of residual hearing. OBJECTIVES: The aim of the study is to evaluate the LCI-20PI electrode array insertion trauma to the intra-cochlear structures in fresh human temporal bone specimens. MATERIALS AND METHODS: The LCI-20PI electrode arrays were inserted into scalae tympani through round window membrane in 10 cochleae from ten fresh human cadavers. The intracochlear trauma was evaluated histologically by a scale of 0-4: 0 - no observable trauma, 1 - elevation of basilar membrane, 2 - rupture of basilar membrane or spiral ligament, 3-dislocation into scala vestibuli and 4 - fracture of modiolus or osseous spiral lamina. The insertion depth was measured by radiography. RESULTS: Histological results revealed no observable trauma in seven specimens; basal membrane elevation and rupture in two specimens; the electrode array misled into scala vestibuli in one specimen. The insertion depth varied from 228° to 288°. CONCLUSIONS AND SIGNIFICANCE: The insertion of the LCI-20PI electrode arrays caused no trauma in the majority of the fresh temporal bone specimens. No translocation of the electrode arrays from the scala tympani to the scala vestibuli was observed.

5.
Br J Cancer ; 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33772151

RESUMO

BACKGROUND: CHK1 is considered an oncogene with overexpression in numerous cancers. However, CHK1 signalling regulation in hepatocellular carcinoma (HCC) remains unclear. METHODS: CHEK1 mRNA, protein, pri-miR-195 and miR-195 expression in HCC tissue was determined by qPCR, WB and IF staining assay. Survival analyses in HCC with high- and low-CHEK1 mRNA expression was performed using TCGA database. Relative luciferase activity was investigated in HCC cells transfected with p-CHEK1 3'UTR. Apoptosis was detected by TUNEL assay. NK and CD8+ T cells were analysed by flow cytometry. RESULTS: CHK1 is increased in human HCC tumours compared with non-cancerous liver. High CHK1 predicts worse prognosis. IFN-γ suppresses CHK1 via IRF-1 in HCC cells. The molecular mechanism of IRF-1 suppressing CHK1 is post-transcriptional by promoting miR-195 binding to CHEK1 mRNA 3'UTR, which exerts a translational blockade. Upregulated IRF-1 inhibits CHK1, which induces apoptosis of HCC cells. Likewise, CHK1 inhibition augments cellular apoptosis in HCC tumours. This effect may be a result of increased tumour NK cell infiltration. However, IRF-1 expression or CHK1 inhibition also upregulates PD-L1 expression via increased STAT3 phosphorylation. CONCLUSIONS: IRF-1 induces miR-195 to suppress CHK1 protein expression. Both increased IRF-1 and decreased CHK1 upregulate cellular apoptosis and PD-L1 expression in HCC.

6.
Cancer Lett ; 506: 95-106, 2021 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-33689775

RESUMO

Interferon regulatory factor 1 (IRF-1) is a tumor suppressor gene in cancer biology with anti-proliferative and pro-apoptotic effect on cancer cells, however mechanisms of IRF-1 regulating tumor microenvironment (TME) in hepatocellular carcinoma (HCC) remain only partially characterized. Here, we investigated that IRF-1 regulates C-X-C motif chemokine 10 (CXCL10) and chemokine receptor 3 (CXCR3) to activate anti-tumor immunity in HCC. We found that IRF-1 mRNA expression was positively correlated with CXCL10 and CXCR3 through qRT-PCR assay in HCC tumors and in analysis of the TCGA database. IRF-1 response elements were identified in the CXCL10 promoter region, and ChIP-qPCR confirmed IRF-1 binding to promote CXCL10 transcription. IRF-2 is a competitive antagonist for IRF-1 mediated transcriptional effects, and overexpression of IRF-2 decreased basal and IFN-γ induced CXCL10 expression. Although IRF-1 upregulated CXCR3 expression in HCC cells, it inhibited proliferation and exerted pro-apoptotic effects, which overcome proliferation partly mediated by activating the CXCL10/CXCR3 autocrine axis. In vitro and in vivo studies showed that IRF-1 increased CD8+ T cells, NK and NKT cells migration, and activated IFN-γ secretion in NK and NKT cells to induce tumor apoptosis through the CXCL10/CXCR3 paracrine axis. Conversely, this effect was markedly abrogated in HCC tumor bearing mice deficient in CXCR3. Therefore, the IRF-1/CXCL10/CXCR3 axis contributes to the anti-tumor microenvironment in HCC.

7.
Transl Oncol ; 14(6): 101059, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33735820

RESUMO

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Lenvatinib oral chemotherapy is approved as a first-line treatment of patients with unresectable HCC. The efficacy and therapeutic duration of lenvatinib are limited by drug resistance, and the mechanism is unclear. IRF2 is a constitutive transcription factor associated with the development of various cancers by regulating cancer cell growth, apoptosis, and drug resistance. However, the potential role of IRF2 in lenvatinib resistance in HCC has not been explored. In this study, we found that IRF2 promoted proliferation, inhibited apoptosis, and increased lenvatinib resistance of HCC cells by regulating ß-catenin expression. Silencing IRF2 downregulated the expression of ß-catenin, while overexpressing IRF2 upregulated ß-catenin. Moreover, the expression of ß-catenin and IRF2 was positively correlated in HCC tissues. Inhibiting ß-catenin with XAV-939 effectively abrogated ß-catenin expression caused by lenvatinib treatment. These findings identify an important function of IRF2 in HCC and demonstrate a mechanism of lenvatinib resistance of HCC cells. Targeting IRF2 may be a potential strategy to improve the therapeutic effect of lenvatinib on HCC.

8.
J Pharm Pharmacol ; 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33779714

RESUMO

OBJECTIVES: The aim of this study was to evaluate the effects of fasudil on insulin resistance (IR) in HTR-8/SVneo cells. METHODS: HTR-8/SVneo cells were treated with insulin or/and fasudil. Cell proliferation, apoptosis, inflammation and related signalling pathways were assessed. KEY FINDINGS: Insulin treatment significantly enhanced the protein expressions of RhoA and Rho kinase (ROCK1 and ROCK2), but decreased glucose consumption. Administration of fasudil effectively promoted glucose uptake. Moreover, fasudil enhanced cell viability and the level of proliferating cell nuclear antigen (PCNA). Insulin-mediated cell apoptosis was inhibited by fasudil via the down-regulation of bax and cleaved-caspase-3, and the up-regulation of bcl-2. At the same time, fasudil led to the reduction of IL-1ß, TNF-α, IL-6 and IL-8 mRNA levels in insulin-treated cells. In addition, RhoA, ROCK2 and phosphorylated myosin phosphatase target subunit-1 (p-MYPT-1) expressions were down-regulated by fasudil. Importantly, fasudil activated insulin receptor substrate-1 (IRS-1) through increasing p-IRS-1 (Tyr612) and p-Akt expressions. The nuclear NF-κB p65 and p-IκB-α levels were reduced via the administration of fasudil in insulin-treated cells. CONCLUSIONS: Fasudil mitigated IR by the promotion of cell proliferation, inhibition of apoptosis and inflammation and regulation of RhoA/ROCK/insulin/NF-κB signalling pathway through in vitro studies.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33435506

RESUMO

The development of low-carbon buildings (LCBs) in China has not reached its expected status, although the Chinese government has formulated many relevant regulations. The real estate developers and consumers are essential participants in the development of LCBs. This paper explores whether the government's implementation of the carbon tax will change their choices of LCBs. Evolutionary game models between developers and consumers are established under static and dynamic carbon taxes. Their evolutionarily stable strategies (ESS) are deduced in different situations. According to the real scenarios in China, numerical simulations are further conducted to show that carbon tax influences the low-carbon behaviors of stakeholders in the construction industry. Under a static carbon tax, the two players cannot reach an equilibrium state, while the game system is stable under a dynamic tax. The probability of the developers constructing LCBs is positively related to the carbon tax, while its degree is gradually weakened as the tax rate increases. Therefore, an appropriate tax should be set to promote the development of LCBs effectively. Finally, policy implications are put forwarded to guide the participants' low-carbon behaviors and reduce the carbon emissions in the Chinese construction industry.


Assuntos
Carbono , Indústria da Construção , Evolução Biológica , China , Humanos , Impostos
10.
Zhonghua Nan Ke Xue ; 26(3): 219-222, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33346960

RESUMO

The novel coronavirus disease 2019 (COVID-19) broke out in December 2019 and has been rapidly escalating throughout the world. Clinical findings show that the patients with either symptomatic or asymptomatic COVID-19 can be a potential source of infection. Although respiratory droplets and close contact are considered to be the main routes of transmission, there is the possibility of aerosol transmission in a relatively closed environment. The nucleic acid of the novel coronavirus can be detected in nasopharyngeal swabs, sputum and other lower respiratory tract secretions, blood, feces, urine and so on, but whether it exists in the semen has not been confirmed. It is reported that the novel coronavirus may affect the testis that highly expresses angiotensin-converting enzyme 2 (ACE2) and theoretically the semen is a possible carrier of the virus considering the fact that it is discharged from the same channel as the urine. Andrology laboratorians are exposed to most of the specimens above, including semen, and some open operations in the laboratory increase the risk of aerosol generation. Therefore, corresponding protective procedures are necessitated in andrology laboratories to reduce the risk of infection during the outbreak of COVID-19. Based on the knowledge and experience available as regards the pandemic and the characteristics of the work in the andrology laboratory, we summarize some biosafety points for andrology laboratorians to attend to during the outbreak of COVID-19.


Assuntos
Andrologia/organização & administração , Contenção de Riscos Biológicos , Laboratórios/organização & administração , Humanos , Masculino
11.
Onco Targets Ther ; 13: 11485-11498, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33204103

RESUMO

Purpose: CD276 protein expression and vasculogenic mimicry (VM) formation are associated with the poor prognosis of hepatocellular carcinoma (HCC) patients. Although both the effects of CD276 and VM formation involve the activation of matrix metalloproteinases, and their relationship has not yet been explored. The following study investigated the effect of CD276 expression on VM formation and the potential mechanisms. Materials and Methods: CD276 expression and VM were examined in commercial tissue microarrays by immunohistochemistry and CD31/PAS double staining. Tumor cell proliferation, invasion, migration and, tube formation were detected in vitro after transfecting HCC cell lines with an shRNA lentiviral vector against CD276. The expression of MMP14, MMP2, VE-cadherin, E-cadherin, and vimentin and MMPs activation was detected by Western blot, immunofluorescence and gelatin zymography assay. In addition, an orthotopic xenograft model of HCC cells was established in vivo, after which VM was detected, along with its marker molecules. Results: CD276 expression was associated with VM and poor prognosis in HCC patients. RNA interference of CD276 reduced tumor cell proliferation, invasion, migration, and VM formation in vitro and in vivo. Furthermore, CD276 knockdown up-regulated the expression of E-cadherin but inhibited the phosphorylation of AKT, the expression of MMP14, MMP2, VE-cadherin, vimentin and the activation of MMP2 and MMP9 in HCC cell lines. Conclusion: CD276 may promote VM formation by activating the PI3K/AKT/MMPs pathway and inducing the EMT process in HCC. CD276 may serve as a promising candidate for the anti-VM treatment of HCC.

12.
PLoS One ; 15(11): e0239119, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33137133

RESUMO

Autophagy is an important factor in liver ischemia-reperfusion injury. In the current study we investigate the function of interferon regulatory factor-1 (IRF1) in regulating autophagy to promote hepatic ischemia reperfusion injury (IR). The high expression of IRF1 during hepatic IR exhibited increased liver damage and was associated with activation of autophagy shown by Western blot markers, as well as immunofluorescent staining for autophagosomes. These effects were diminished by IRF1 deficiency in IRF1 knock out (KO) mice. Moreover, the autophagy inhibitor 3-MA decreased IR-induced liver necrosis and markedly abrogated the rise in liver injury tests (AST/ALT). ß-catenin expression decreased during liver IR and was increased in the IRF1 KO mice. Immunoprecipitation assay showed the binding between IRF1 and ß-catenin. Overexpression of IRF1 induced autophagy and also inhibited the expression of ß-catenin. ß-catenin inhibitor increased autophagy while ß-catenin agonist suppressed autophagy in primary mouse hepatocytes. These results indicate that IRF1 induced autophagy aggravates hepatic IR injury in part by inhibiting ß-catenin and suggests that targeting IRF1 may be an effective strategy in reducing hepatic IR injury.

13.
J Xray Sci Technol ; 2020 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-33044222

RESUMO

BACKGROUND: Dual-energy breast CT reconstruction has a potential application that includes separation of microcalcification from healthy breast tissue for assisting early breast cancer detection. OBJECTIVE: To investigate and validate the noise suppression algorithm applied in the decomposition of the simulated breast phantom into microcalcification and healthy breast. METHODS: The proposed hybrid optimization method (HOM) uses a simultaneous algebraic reconstruction technique (SART) output as a prior image, which is then incorporated into the self-adaptive dictionary learning. This self-adaptive dictionary learning seeks each group of patches to faithfully represent the learned dictionary, and the sparsity and non-local similarity of group patches are used to enforce the image regularization term of the prior image. We simulate a numerical phantom by adding different levels of Gaussian noise to test performance of the proposed method. RESULTS: The mean value of peak signal-to-noise ratio (PSNR), structural similarity (SSIM), and root mean square error (RMSE) for the proposed method are (49.043±1.571), (0.997±0.002), (0.003±0.001) and (51.329±1.998), (0.998±0.002), (0.003±0.001) for 35 kVp and 49 kVp, respectively. The PSNR of the proposed method shows greater improvement over TWIST (5.2%), SART (34.6%), FBP (40.4%) and TWIST (3.7%), SART (39.9%), FBP (50.3%) for 35 kVp and 49 kVp energy images, respectively. For the proposed method, the signal-to-noise ratio (SNR) of decomposed normal breast tissue (NBT) is (22.036±1.535), which exceeded that of TWIST, SART, and FBP by 7.5%, 49.6%, and 96.4%, respectively. The results reveal that the proposed algorithm achieves the best performance in both reconstructed and decomposed images under different levels of noise and the performance is due to the high sparsity and good denoising ability of minimization exploited to solve the convex optimization problem. CONCLUSIONS: This study demonstrates the potential of applying dual-energy reconstruction in breast CT to detect and separate clustered MCs from healthy breast tissues without noise amplification. Compared to other competing methods, the proposed algorithm achieves the best noise suppression performance for both reconstructed and decomposed images.

14.
Nucleic Acids Res ; 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33045751

RESUMO

Expression profiles of long non-coding RNAs (lncRNAs) across diverse biological conditions provide significant insights into their biological functions, interacting targets as well as transcriptional reliability. However, there lacks a comprehensive resource that systematically characterizes the expression landscape of human lncRNAs by integrating their expression profiles across a wide range of biological conditions. Here, we present LncExpDB (https://bigd.big.ac.cn/lncexpdb), an expression database of human lncRNAs that is devoted to providing comprehensive expression profiles of lncRNA genes, exploring their expression features and capacities, identifying featured genes with potentially important functions, and building interactions with protein-coding genes across various biological contexts/conditions. Based on comprehensive integration and stringent curation, LncExpDB currently houses expression profiles of 101 293 high-quality human lncRNA genes derived from 1977 samples of 337 biological conditions across nine biological contexts. Consequently, LncExpDB estimates lncRNA genes' expression reliability and capacities, identifies 25 191 featured genes, and further obtains 28 443 865 lncRNA-mRNA interactions. Moreover, user-friendly web interfaces enable interactive visualization of expression profiles across various conditions and easy exploration of featured lncRNAs and their interacting partners in specific contexts. Collectively, LncExpDB features comprehensive integration and curation of lncRNA expression profiles and thus will serve as a fundamental resource for functional studies on human lncRNAs.

15.
Laryngoscope ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33030216

RESUMO

OBJECTIVES: To determine the clinical characteristics of papilloma involving the external auditory canal (PEAC) in a region of China. STUDY DESIGN: A retrospective study. METHODS: Demographics, manifestations, imaging results, histopathology, and treatment of 67 patients diagnosed with PEAC in a period of 6 years were analyzed at Shanghai Eye, Ear, Nose and Throat Hospital in China. RESULTS: PEAC were encountered in patients between the ages of 12 and 82 years (mean 53.8 years). It was more prevalent in men (82%) than in women (18%) (P < .05). The clinical presentation was usually a mass in EAC, aural fullness, and hearing loss. Otoscopic and radiological examination were used together for initial diagnosis and pretreatment planning. Unilateral involvement was more common than bilateral involvement (P < .05). The average time between onset of first symptom and surgical resection and/or biopsy was 6.5 months (range, 0.25-60 months). All patients underwent gross total resection. In 5 patients, (7.5%) carcinoma was detected in the specimen. Fifteen patients (22%) had recurrence; recurrent tumors were detected after an average period of 10 months after surgery (range, 4-24 months). CONCLUSION: PEAC is largely a benign lesion with a low risk of malignancy. Optimal management is via gross total resection. However, the risk of recurrence is high, which motivates a need for long-term monitoring. LEVEL OF EVIDENCE: 4 Laryngoscope, 2020.

16.
Materials (Basel) ; 13(17)2020 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-32872088

RESUMO

This study investigated fundamental mechanical properties of a basalt fiber reinforced engineered cementitious composite (BF-ECC) with different volume fractions of basalt fiber (BF), water-binder ratio (W/B) and fly ash (FA) content. The compressive strength, splitting tensile strength, flexural strength and static modulus of BF-ECC were studied at 3, 28 and 56 days, respectively, to explore their development along the ages. Furthermore, the scanning electron microscopy (SEM) analysis was conducted to evaluate the microstructure of BF-ECC. Experiment results demonstrated that bond quality between the BF and the matrix is good, which leads to a significant increase in the flexural strength and splitting tensile strength. The pozzolanic effect of FA obviously improved the splitting tensile and flexural strength of BF-ECC after 56 days of curing, and the appropriate content of the FA content in the BF-ECC ranges from 50% to 60%.

17.
Artigo em Inglês | MEDLINE | ID: mdl-32812154

RESUMO

Evaluating carbon emission performance of the construction industry is a significant prerequisite for developing regional carbon mitigation plans. Taking environmental and technical heterogeneities into account, this paper employed a meta-frontier method to measure the carbon emission efficiency, carbon mitigation potential, and costs of the construction sector in different regions of China from 2005 to 2016. The empirical results show that substantial disparities in carbon emission efficiency exist in the construction industry. The total carbon mitigation potential of this sector was 206.76 million tons, with the Lower Yellow river area accounting for the largest proportion at 27%. Meanwhile, the carbon mitigation costs of this sector increased from 584.94 to 1273.30 yuan/ton during 2005-2016. The highest mitigation costs occur in the Lower Yangtze River area and the South Coastal area, indicating it was more costly in these areas to conduct additional carbon emissions mitigation. The results could facilitate the policy formulation on regional-oriented carbon emissions mitigation of the construction industry in China.

18.
Biology (Basel) ; 9(7)2020 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-32630734

RESUMO

Amphibian skin secretions are remarkable sources of novel bioactive peptides. Among these, antimicrobial peptides have demonstrated an outstanding efficacy in killing microorganisms via a general membranolytic mechanism, which may offer the prospect of solving specific target-driven antibiotic resistance. Here, the discovery of a novel defensive peptide is described from the skin secretion of the African frog, Kassina senegalensis. Named kassinatuerin-3, it was identified through a combination of "shot-gun" cloning and MS/MS fragmentation sequencing. Subsequently, a synthetic replicate was subjected to biofunctional evaluation. The results indicated that kassinatuerin-3 possessed antimicrobial activity against Gram-positive bacteria but no effect against Gram-negative bacteria. Additionally, it was active in biofilm eradication on S. aureus and MRSA and in the antiproliferation of selected cancer cell lines. Moreover, it had a very mild hemolytic effect, which demonstrated a high therapeutic index for kassinatuerin-3. Collectively, although kassinatuerin-3 did not demonstrate remarkable bioactivities compared with other natural or synthetic antimicrobial peptides (AMPs), it offered a new insight into the design of antimicrobial derivatives.

19.
Asia Pac J Clin Oncol ; 16(5): e236-e241, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32573088

RESUMO

AIM: Cervical basaloid squamous cell carcinoma (BSCC) is a rare variant of squamous cell carcinoma with little recognition. Our objective was to identify difference in characteristics and outcomes between BSCC and squamous cell carcinoma, usual type (SSC, UT). METHODS: A total of 32 BSCC patients and 6387 SCC, UT patients, diagnosed from 2004 to 2014, were identified using the Surveillance, Epidemiology, and End Results database. RESULTS: More BSCC patients presented with older age as compared to SCC, UT (patients over 40 years old: 87.5% vs 60.5%, P  < .01). Univariate survival analysis shows that overall survival (OS) and disease-specified survival (DSS) in the BSCC group were slightly better than SCC, UT group, but without statistical significance. Furthermore, BSCC presented similar prognosis in both DSS and OS compared to SCC, UT when matching other parameters with the propensity score matching methods. CONCLUSION: BSCC tends to appear in older people. However, BSCC appears to carry a similar prognosis compared to SCC, UT.


Assuntos
Carcinoma de Células Escamosas/fisiopatologia , Colo do Útero/patologia , Adolescente , Adulto , Idoso , Carcinoma de Células Escamosas/mortalidade , Bases de Dados Factuais , Feminino , Humanos , Pessoa de Meia-Idade , Prognóstico , Programa de SEER , Adulto Jovem
20.
Transl Oncol ; 13(10): 100805, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32563177

RESUMO

Hepatocellular carcinoma (HCC) is often treated with doxorubicin. MicroRNAs have been shown to have important regulatory roles in cancer and serve as a target in chemoresistance. In this study, we investigated the effects of specific microRNA-200a (miR-200a) on HCC tumor cell growth and effect of doxorubicin-mediated cytotoxicity. Our results show miR-200a is downregulated in human HCC and HCC tumor cell lines. Increasing miR-200a expression inhibited HCC growth and synergized with the antitumor effects of doxorubicin. Inhibiting endogenous miR-200a promoted tumor growth and chemotherapeutic resistance. Increasing miR-200a expression inhibited tumor metabolism (ATP production, mitochondrial respiration, glycolysis), while inhibition of endogenous miR-200a reversed these effects. MiR-200a expression also increased autophagy and synergized with doxorubicin-mediated cytotoxicity. This study identifies a novel role of miR-200a in potentiating doxorubicin-mediated therapeutic effects in HCC.

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