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1.
Chemosphere ; 286(Pt 2): 131680, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34365166

RESUMO

In this study, a BDD electrolytic oxidation-ceramic membrane ultrafiltration (EO-CM) system for the removals of antibiotics, organic matters and ammonia in wastewater was evaluated. Sulfamethazine (SMZ) was degraded following a pseudo first-order kinetics. The removal rate of SMZ improved with the increase of electro-oxidation time (0-60 min) and current density (5-30 mA/cm2). During the BDD electro-oxidation process, H2O2 and hydroxyl radicals (•OH) were generated which were detected by N, N-diethyl-p-phenylenediamine (DPD) method and electron paramagnetic resonance spectroscopy (EPR), respectively. Chemical oxygen demand (COD) was able to be removed by EO and CM processes, in which proteins and humic acids were regarded as the main removed components measured using excitation-emission matrix (EEM) technique. Moreover, BDD electro-oxidation pretreatment could make the CM process maintain a high water flux and significantly control the membrane fouling and relieve transmembrane pollution. In addition, the removal of ammonia was enhanced with the increase of chloride ions (Cl-) in wastewater during EO process due to the generation of active chlorine (i.e., ClO-, HClO, or Cl2) from the oxidation of Cl-. Chloramine and nitrogen were produced in the oxidation of ammonia by active chlorine. Overall, the results of this study suggest that BDD EO-CM system is a promising process for removing antibiotics, organic matters and ammonia.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Amônia , Antibacterianos , Boro , Cerâmica , Diamante , Eletrodos , Peróxido de Hidrogênio , Oxirredução , Ultrafiltração , Poluentes Químicos da Água/análise
2.
J Anim Sci Biotechnol ; 12(1): 103, 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34615552

RESUMO

BACKGROUND: Follicular atresia has been shown to be strongly associated with a low follicle utilization rate and female infertility, which are regulated by many factors such as microRNAs (miRNAs), which constitute a class of noncoding RNAs (ncRNAs). However, little is known about long noncoding RNAs (lncRNAs), which constitute another ncRNA family that regulate follicular atresia. RESULTS: A total of 77 differentially expressed lncRNAs, including 67 upregulated and 10 downregulated lncRNAs, were identified in early atretic follicles compared to healthy follicles by RNA-Sequencing. We characterized a noncoding RNA that was highly expressed in atretic follicles (NORHA). As an intergenic lncRNA, NORHA was one of the upregulated lncRNAs identified in the atretic follicles. To determine NORHA function, RT-PCR, flow cytometry and western blotting were performed, and the results showed that NORHA was involved in follicular atresia by influencing GC apoptosis with or without oxidative stress. To determine the mechanism of action, bioinformatics analysis, luciferase reporter assay and RNA immunoprecipitation assay were performed, and the results showed that NORHA acted as a 'sponge', that directly bound to the miR-183-96-182 cluster, and thus prevented its targeted inhibition of FoxO1, a major sensor and effector of oxidative stress. CONCLUSIONS: We provide a comprehensive perspective of lncRNA regulation of follicular atresia, and demonstrate that NORHA, a novel lncRNA related to follicular atresia, induces GC apoptosis by influencing the activities of the miR-183-96-182 cluster and FoxO1 axis.

3.
Environ Res ; : 112178, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34624270

RESUMO

Non-phase-separated hexagonal boron carbon nitride (h-BCN) is an emerging type of promising metal-free photocatalyst, but the synthesis of this material remains quite challenging. Here, h-BCN without phase separation was obtained through a novel organic-inorganic hybrid precursor pyrolysis method using boric acid and ethylenediamine as raw materials. The resultant BCN-1 exhibited excellent photocatalytic activity for CO2 reduction, as confirmed by a CO generation rate of 13.97 µmol g-1 h-1 under visible light illumination with no co-catalyst or sacrificial agent. This rate was 9.4 times higher than that of g-C3N4 (2.1 µmol g-1 h-1) under the same experimental condition. The pre-existing C-N-B bond is essential for mediating the growth kinetics and diminishing the thermodynamically preferred C and BN phase-segregation structure, while ammonia is crucial for C-N-B bond fixation and pore formation during the pyrolysis process. This finding of a facile method for synthesizing non-phase-separated BCN has positive effects on the study of photocatalytic CO2 reduction by sustainable metal-free catalysts.

4.
J Zhejiang Univ Sci B ; 22(10): 839-855, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-34636187

RESUMO

B cell lymphoma 2 (Bcl-2) is an important antiapoptotic gene that plays a dual role in the maintenance of the dynamic balance between the survival and death of cancer cells. In our previous study, Bcl-2 was shown to delay the G0/G1 to S phase entry by regulating the mitochondrial metabolic pathways to produce lower levels of adenosine triphosphate (ATP) and reactive oxygen species (ROS). However, the detailed molecular mechanisms or pathways by which Bcl-2 regulates the cell cycle remain unknown. Here, we compared the effects of Bcl-2 overexpression with an empty vector control in the NIH3T3 cell line synchronized by serum starvation, and evaluated the effects using proteomic analysis. The effect of Bcl-2 on cell cycle regulation was detected by monitoring Bcl-2 and p27 expression. The result of subsequent proteomic analysis of Bcl-2 overexpressing cells identified 169 upregulated and 120 downregulated proteins with a 1.5-fold change. These differentially expressed proteins were enriched in a number of signaling pathways predominantly involving the ribosome and oxidative phosphorylation, according to the data of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. These results indicated that Bcl-2 potentially acts at the translation level to influence proteins or enzymes of the respiratory chain or in the ribosome, and thereby regulates the cell cycle. Additionally, differentially expressed proteins involved in oxidative phosphorylation were determined to account for most of the effects of Bcl-2 on the cell cycle mediated by the mitochondrial pathway investigated in our previous study. These results can provide assistance for additional in-depth studies on the regulation of the cell cycle by Bcl-2. The results of the proteomic analysis determined the mechanism of Bcl-2-dependent delay of the cell cycle progression. In summary, the results of this study provide a novel mechanistic basis for identifying the key proteins or pathways for designing and developing precisely targeted cancer drugs.

5.
Cell Death Discov ; 7(1): 253, 2021 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-34537818

RESUMO

Transforming growth factor-ß1 (TGF-ß1) is essential for ovarian function and female fertility in mammals. Herein, we identified three completely linked variants, including two known variants referred to as c.1583A > G and c.1587A > G and the novel variant c.2074A > C in the porcine TGF-ß1 3'-UTR. An important role of these variants in Yorkshire sow fertility was revealed. Variants c.1583A > G and c.1587A > G were located at the miRNA response element (MRE) of miR-2337 and affected miR-2337 regulation of TGF-ß1 3'-UTR activity. Interestingly, miR-2337 induces, not reduces the transcription and production of TGF-ß1 in granulosa cells (GCs). Mechanistically, miR-2337 enhances TGF-ß1 promoter activity via the MRE motif in the core promoter region and alters histone modifications, including H3K4me2, H3K4me3, H3K9me2, and H3K9ac. In addition, miR-2337 controls TGF-ß1-mediated activity of the TGF-ß signaling pathway and GC apoptosis. Taken together, our findings identify miR-2337 as an endogenous small activating RNA (saRNA) of TGF-ß1 in GCs, while miR-2337 is identified as a small activator of the TGF-ß signaling pathway which is expected to be a new target for rescuing GC apoptosis and treating low fertility.

6.
ACS Appl Mater Interfaces ; 13(39): 46598-46607, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34553598

RESUMO

The low charge-transfer efficiency and slow surface reaction kinetics are the main factors affecting the performance of carbon nitride photocatalysts. Here, a Schottky heterostructure (WCN) was constructed by combining WC with porous carbon nitride nanosheets with a cyanide group (NCN). The Schottky junction provides a convenient way for photoinduced electrons to transfer and promotes the effective separation of photoinduced carriers. Furthermore, due to the good conductivity of WC and an electronic structure similar to Pt, the W atom in WC as the active site of hydrogen production can realize efficient reaction kinetics. In this way, the WCN Schottky heterostructure showed a 2.0- and 5.0-fold enhancement in photocatalytic H2 evolution as compared to the single NCN component under visible-light and near-infrared light irradiation. By combining with theoretical simulations, as an electron acceptor in the WCN heterostructure, WC can effectively improve the charge-transfer efficiency and also act as an active site for hydrogen production.

7.
Blood Adv ; 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34559203

RESUMO

Hyperhomocysteinemia (HHcy) is associated with an exaggerated platelet thrombotic response at sites of vascular injury. Here, a human medical examination report showed that elevated human plasma Hcy levels were positively correlated with enhanced blood coagulation and platelet activity, suggesting that humans with HHcy are more prone to thrombus formation at the sites of vascular injury. Accordingly, we observed accelerated platelet activation, primary hemostasis, and thrombus formation both in acute and chronic HHcy ApoE-/- mice. Upon Hcy administration in C57BL/6J mice, platelet aggregation, spreading, and clot retraction were markedly promoted. More importantly, homocysteine (Hcy) increased the affinity of platelet integrin αIIbß3 with ligands and enhanced integrin outside-in signaling by promoting membrane phosphatidylserine (PS) exposure in vitro. Mechanistically, lipidomics analysis showed that lysophosphatidylcholines were the primary metabolites leading to clustering of HHcy-stimulated platelets. Cytosolic phospholipase A2 (cPLA2) activity and autotaxin (ATX, a secreted lysophospholipase D) secretion were upregulated by Hcy, leading to membrane phospholipid hydrolysis and PS exposure. Moreover, secreted ATX directly interacted with integrin ß3. Inhibitors of cPLA2 and ATX activity blocked integrin αIIbß3 outside-in signaling and thrombosis in HHcy ApoE-/- mice. This study identifies a novel mechanism by which HHcy promotes platelet membrane phospholipid catabolism and extracellular ATX secretion to activate integrin outside-in signaling, consequently to exaggerate thrombosis. This study reveals an innovative approach to treat HHcy-related thrombotic diseases.

8.
Lancet ; 398(10306): 1131, 2021 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-34563292
9.
Int J Mol Sci ; 22(18)2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34576190

RESUMO

TGF-ß family signaling pathways, including TGF-ß and BMP pathways, are widely involved in the regulation of health and diseases through downstream SMADs, which are also regulated by multiple validated mechanisms, such as genetic regulation, epigenetic regulation, and feedback regulation. However, it is still unclear whether R-SMADs or Co-SMAD can feedback regulate the TGF-ß family signaling pathways in granulosa cells (GCs). In this study, we report a novel mechanism underlying the feedback regulation of TGF-ß family signaling pathways, i.e., SMAD4, the only Co-SMAD, positive feedback activates the TGF-ß family signaling pathways in GCs with a basal level of TGF-ß ligands by interacting with the core promoters of its upstream receptors. Mechanistically, SMAD4 acts as a transcription factor, and feedback activates the transcription of its upstream receptors, including ACVR1B, BMPR2, and TGFBR2, of the canonical TGF-ß signaling pathways by interacting with three coactivators (c-JUN, CREB1, and SP1), respectively. Notably, three different interaction modes between SMAD4 and coactivators were identified in SMAD4-mediated feedback regulation of upstream receptors through reciprocal ChIP assays. Our findings in the present study indicate for the first time that SMAD4 feedback activates the canonical TGF-ß family signaling pathways in GCs, which improves and expands the regulatory mechanism, especially the feedback regulation modes of TGF-ß family signaling pathways in ovarian GCs.


Assuntos
Ovário/metabolismo , Proteína Smad4/metabolismo , Receptores de Ativinas Tipo I/genética , Receptores de Ativinas Tipo I/metabolismo , Animais , Apoptose/genética , Apoptose/fisiologia , Western Blotting , Receptores de Proteínas Morfogenéticas Ósseas Tipo II/genética , Receptores de Proteínas Morfogenéticas Ósseas Tipo II/metabolismo , Imunoprecipitação da Cromatina , Biologia Computacional , Epigênese Genética/genética , Epigênese Genética/fisiologia , Feminino , Humanos , Imunoprecipitação , Regiões Promotoras Genéticas/genética , Reação em Cadeia da Polimerase em Tempo Real , Receptor do Fator de Crescimento Transformador beta Tipo II/genética , Receptor do Fator de Crescimento Transformador beta Tipo II/metabolismo , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Proteína Smad4/genética , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/metabolismo
10.
Ann Palliat Med ; 10(9): 9372-9382, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34412495

RESUMO

BACKGROUND: This investigation established a scoring scale for predicting the incidence of postoperative complications in patients after spinal tuberculosis debridement. METHODS: A total of 232 spinal tuberculosis patients who underwent debridement surgery between January 2012 to May 2020 were included in this retrospective study. The study cohort was divided into 2 groups according to the presence or absence of postoperative complications. The complications were defined as abnormal or impaired body function caused by surgical factors (such as nerve injury and internal fixation looseness) or other factors (such as chemotherapy and bed stay). Clinical characteristics include age, body mass index (BMI), diabetes mellitus, pulmonary tuberculosis, smoking history, preoperative serum albumin, preoperative C-reactive protein (CRP), Cobb angle correction, surgical approach, operation time, etc. operative blood loss was recorded and analyzed. The clinical characteristics of spinal tuberculosis patients who suffered postoperative complications were evaluated, and a scoring scale was established using logistic regression analysis. The performance of this scoring scale was prospectively validated. RESULTS: Out of 232 patients, a total of 188 (81.03%) suffered postoperative complications after surgery. Multivariate binary logistic regression analysis showed that diabetes mellitus [adjusted odds ratio (OR) =1.110, P=0.046], pulmonary tuberculosis (adjusted OR =1.181, P=0.002), low preoperative serum albumin (adjusted OR =0.789, P=0.005), anterior surgical approach (adjusted OR =5.934, P=0.035), and long operation time (adjusted OR =1.019, P<0.01) were independent risk factors of postoperative complications after spinal tuberculosis debridement surgery. The above independent risk factors were assigned to establish a scoring scale for predicting postoperative complications, and receiver operating characteristic (ROC) analysis showed that the optimal cut-off value for the scoring scale was 4 points. The sensitivity and specificity of the scoring scale were 60.8% and 81.8%, respectively, based on the validation set. CONCLUSIONS: Using the scoring scale, spinal tuberculosis patients with a score between 4 to 9 would be considered at high risk of postoperative complications, while patients with a score of 0 to 3 would likely be at low risk of developing postoperative complications.


Assuntos
Fusão Vertebral , Tuberculose da Coluna Vertebral , Estudos de Coortes , Desbridamento , Humanos , Vértebras Lombares , Complicações Pós-Operatórias/etiologia , Estudos Retrospectivos , Fatores de Risco , Vértebras Torácicas , Resultado do Tratamento
11.
Mol Ther Nucleic Acids ; 25: 251-263, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34458009

RESUMO

Antisense long noncoding RNAs (AS-lncRNAs), a sub-class of lncRNAs, are transcribed in the opposite direction from their overlapping protein-coding genes and are implicated in various physiological and pathological processes. However, their role in female reproduction remains largely unknown. Here, we report that BRE-AS, an AS-lncRNA transcript from intron 10 of the protein-coding gene BRE, is involved in granulosa cell (GC) apoptosis. Based on our previous RNA sequencing data, we identified 28 AS-lncRNAs as important in the initiation of porcine follicular atresia, with BRE-AS showing the most significant upregulation in early atretic follicles. In this study, gain- and loss-of-function assays demonstrated that BRE-AS induces early apoptosis in GCs. Mechanistically, BRE-AS acts in cis to suppress the expression of BRE, an anti-apoptotic factor, via direct interaction with the pre-mRNA transcript of the latter, inducing increased GC apoptosis. Notably, we also found that BRE-AS was upregulated in SMAD4-silenced GCs. SMAD4 was identified as a transcriptional repressor of BRE-AS because it inhibits BRE-AS expression and BRE-AS-mediated GC apoptosis. In conclusion, we not only identified a novel AS-lncRNA related to the early apoptosis of GCs and initiation of follicular atresia but also described a novel regulatory pathway, SMAD4/BRE-AS/BRE, coordinating GC function and female fertility.

12.
J Fungi (Basel) ; 7(6)2021 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-34207925

RESUMO

During the investigation of endophytic fungi diversity in aquatic plants and the fungal diversity in soil in southwest China, we obtained 208 isolates belonging to Trichoderma, including 28 isolates as endophytes from aquatic plants and 180 isolates as saprobes from soil, respectively. Finally, 23 new species of Trichoderma are recognized by further studies. Their phylogenetic positions are determined by sequence analyses of the combined partial sequences of translation elongation factor 1-alpha (tef1) and gene encoding of the second largest nuclear RNA polymerase subunit (rpb2). The results revealed that the 23 new species are distributed in nine known clades. The morphology and culture characteristics are observed, described and illustrated in detail. Distinctions between the new species and their close relatives were compared and discussed. These include: Trichoderma achlamydosporum, T. amoenum, T. anaharzianum, T. anisohamatum, T. aquatica, T. asiaticum, T. asymmetricum, T. inaequilaterale, T. inconspicuum, T. insigne, T. obovatum, T. paraviride, T. pluripenicillatum, T. propepolypori, T. pseudoasiaticum, T. pseudoasperelloides, T. scorpioideum, T. simile, T. subazureum, T. subuliforme, T. supraverticillatum, T. tibetica, and T. uncinatum.

13.
Reprod Sci ; 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34287793

RESUMO

The miR-183-96-182 cluster is a polycistronic miRNA cluster necessary for ovarian functions in mammals. However, its transcriptional regulation in the ovary is largely unclear. In this study, we characterized the promoter region of the porcine miR-183-96-182 cluster, and showed that SMAD4 may function as a transcriptional activator of the miR-183-96-182 cluster in GCs through direct binding to SBE motifs in its promoter. SMAD4 may inhibit GC apoptosis via suppression of FoxO1, an effector of GC apoptosis and a direct target of the miR-183-96-182 cluster, by inducing the miR-183-96-182 cluster, and this process may be regulated by the TGF-ß/SMAD signaling pathway. Our findings uncovered the regulatory mechanism of miR-183-96-182 cluster expression in GCs and demonstrated that TGF-ß1/SMAD4/miR-183-96-182 cluster/FoxO1 may be a potential pathway for regulating follicular atresia and female reproduction.

14.
Environ Sci Pollut Res Int ; 28(42): 59963-59973, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34152543

RESUMO

Industrial dyeing produces highly polluting wastewater and threatens the environment. Effective treatment of dyeing wastewater is a crucial step to prevent toxic chemicals from entering receiving waters. This study aimed to assess a modified attapulgite (ATP)-based adsorbent for dyeing wastewater purification by introducing chitosan (CTS) and titanium dioxide (TiO2) into the adsorbent material named TiO2/CTS/ATP. It was found that after modification, the adsorbent showed a lower hydrophilicity, as demonstrated by an increase in the water contact angle from 9.1° to 42°, which could reduce the water adsorption tendency and potentially facilitate contaminants adherence. The modification also led to a significantly increased specific surface area of 79.111 m2/g from 3.791 m2/g and exhibited more uniform and smaller particle size (reduced from 3.99 to 2.52 µm). When the TiO2/CTS/ATP adsorbent was applied to the adsorption of Congo red solution, the adsorption efficiency was observed to reach to 97.6% at the dosage of 0.5 g/L. Furthermore, the combination of adsorption and ultrafiltration was able to achieve 99% Congo red removal. Adsorption pretreatment prior to the ultrafiltration also enabled to reduce membrane fouling, increased the reversible membrane fouling, and resulted in a considerably lower transmembrane pressure as compared with the direct ultrafiltration filtration system.


Assuntos
Quitosana , Poluentes Químicos da Água , Purificação da Água , Adsorção , Corantes , Compostos de Magnésio , Compostos de Silício , Titânio , Ultrafiltração
15.
Chemosphere ; 279: 130603, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34134412

RESUMO

Achieving adequate manganese removal during water treatment is a challenging process. This study aimed to assess the effectiveness of gravity driven ceramic membrane (GDCM) filtration in the elimination of manganese from surface water. The impact of membrane pre-modification with birnessite and molecular weight cut-off on long-term water treatment efficiency was investigated by assessing filtration units with 300 kDa virgin membrane (300 kDa-blank), 300 kDa membrane pre-coated with manganese oxides (300 kDa-MnOx), and 15 kDa virgin membrane (15 kDa-blank). The results of 300 kDa-blank and 300 kDa-MnOx indicated that depositing manganese oxides (produced via ozone (O3) oxidation) prior to water treatment was conducive to ripening of cake layer which played a major role in Mn removal. Reducing membrane molecular cut-off from 300 to 15 kDa also significantly reduced permeate Mn concentration, achieving a removal efficiency of 75% at the end of the trial (highest of all the units). Relative to 300 kDa-blank, the greater manganese removals in the other two systems can be attributed to 1) the long hydraulic retention times resulting from the higher membrane resistance, and 2) the higher abundance of biologically produced Birnessite materials in the cake layers for manganese oxidation. Raman analysis and X-ray diffraction analysis showed that 15 kDa-blank achieved the highest level of Birnessite production and most cake materials featured a flower-like structure and relatively small size (as shown under a scanning electron microscope and Energy Dispersive X-Ray Spectroscopy element mapping analysis), suggesting a higher surface area for Mn oxidation.


Assuntos
Ozônio , Purificação da Água , Cerâmica , Filtração , Manganês , Compostos de Manganês , Oxirredução , Óxidos , Água
16.
Dis Markers ; 2021: 6640254, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34136021

RESUMO

Objective: Spinal tuberculosis (TB) misdiagnosed of spinal metastasis was not rarely reported, especially in outpatients department. This study was aimed to establish an outpatient scoring system to preliminarily distinguish spinal metastasis from spinal TB. Methods: We retrospectively reviewed consecutive 141 patients with a pathological diagnosis of spinal metastasis (82 cases) or spinal TB (59 cases) in our hospital from January 2017 to June 2018. The following clinical characteristics which can be obtained by outpatient orthopedist were recorded and analyzed: age, gender, malignant tumor history, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and imaging features including distribution characteristics of vertebral lesions, subligamentous spread, paravertebral or psoas abscess, involved vertebral element, intervertebral disc, and sequestra formation. The prevalence of clinical characteristics in spinal metastasis was evaluated, and the scoring system was established using logistic regression analysis. The performance of the scoring system was also prospectively validated. Results: The outpatient scoring system was based on five clinical characteristics confirmed as significant predictors of spinal metastasis, namely, malignant tumor history, subligamentous spread, posterior element lesions, preserved discs, and no sequestra formation. Spinal metastasis showed a significant higher score than spinal TB (8.17 points vs. 1.97 points, t = 18.621, P < 0.001), and the optimal cut-off value for the scoring system was 5 points. The sensitivity and specificity of the scoring system for predicting spinal metastasis were 97.85% and 88.33%, respectively, in the validation set. Conclusion: Spinal lesions with the score of 5 to 10 would be considered a diagnosis of spinal metastasis, while the score of 0 to 4 may be spinal TB. Because the scoring system is mainly based on the clinical characteristics that can be obtained by an outpatient orthopedist, it is suitable to be used as a diagnostic tool in the outpatient department.

17.
Sci Total Environ ; 783: 146990, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34088166

RESUMO

Nanofiltration (NF) concentrate generated from the secondary wastewater treatment contains high concentration of ammonium nitrogen and refractory organics, thus having great environmental risks. In this study, an electro-oxidation (EO) reactor built up with a boron-doped diamond (BDD) anode is utilized to treat the NF concentrate. To reach "zero liquid discharge", a mixture of the electrolytic effluent and the raw secondary wastewater was collected and transported back to the NF module. Results show that under the current density of 30 mA·cm-2, most of ammonia nitrogen was decomposed into N-gases within 30 min due to the active chlorine radicals generated in the electrochemical process. Moreover, the EO reactor completely eliminated antibiotics, humic acids and bacteria in the NF concentrate under long electrolysis time of 60 min. In particular, the organic pollutants removal rate was kept at a stable value in the EO reactor for a long-term operation of up to 120 h. In addition, the NF membrane remained a constant permeate flux without being affected by the membrane biofouling caused by organic components in wastewater. Our study highlights the potential of the NF-EO process as a "zero liquid discharge" approach for treatment of the secondary wastewater.

18.
Anal Chem ; 93(22): 7843-7850, 2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34029050

RESUMO

Frequently calibrating electrochemical biosensors (ECBs) to obtain acceptable accuracy can be cumbersome for the users. Thus, the achievement of calibration-free operation would effectively lead to commercial applications for ECBs in the real world. Herein, we fabricated a temperature-alternated electrochemical aptamer-based (TAEAB) sensor, producing a cycle of "enhanced-responsive and ∼nonresponsive" state at rapidly alternated interface temperatures (5 and 30 °C, respectively). The ratio of peak currents collected at two temperatures overcomes sensor-to-sensor fabrication variations, obviating sensor calibration prior to use due to its good reproducibility. We then demonstrated the capability of TAEAB sensors for improved, sensitive, and calibration-free measurement of different targets within 7 min, which respectively achieved a detection limit of 0.5 µM procaine in undiluted urine and 1.0 µM adenosine triphosphate in undiluted serum. This generalizable approach ameliorates sensitivity without the complicated amplification step, thus simplifying the operation procedure and reducing the detection time, which will effectively improve the clinical utility of biosensors.


Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Calibragem , Técnicas Eletroquímicas , Reprodutibilidade dos Testes , Temperatura
19.
Dis Markers ; 2021: 5584372, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33968282

RESUMO

Purpose: A retrospective imaging study assessing the availability of oblique lumbar interbody fusion at the level of L5-S1 (OLIF51) and to choose ideal surgical corridor in OLIF51 by introducing V-line. Methods: The axial views through the center of L5-S1 disc were reviewed. We adopt 18 mm as the width of the simulated surgical corridor. The midline of the surgical corridor is at the center of L5-S1 disc. According to the traction distance of the left iliac vein (LCIV) and psoas major (PM), we defined all the subjects as V (+) (traction-difficultly LCIV), V (-) (traction-friendly LCIV), P (+) (traction-difficultly PM), and P (-) (traction-friendly PM). V-line was defined as a straight line dividing equally the simulated surgical corridor. All cases were divided into 2 groups: The V-line (+) group, more than half of the LCIV region, is located in the ventral part of V-line; the V-line (-) group, more than half of the LCIV region, is located in the dorsal part of V-line. Multiple variables regressive analysis was conducted to analyze the independent risk factors of V-line (+). Results: V-line (+) was found in 36 (38.7%) patients and V-line (-) in 57 (61.3%). Incidence of V (+) and P (+) was 35.4% (33/93) and 30.1% (28/93), respectively. 16.1% (15/93) subjects processed V (+) and P (+) at the same time. The independent risk factor of V-line (+) were gender of male (P = 0.034, OR: 12.152) and medial position of LCIV (P < 0.001, OR: 265.085). High iliac crest was a significant independent protective factor (P = 0.001, OR: 0.750). Conclusions: Most patients were suitable for OLIF51. V-line could assess the injury risk of LCIV. For patients who are V-line (+), mainly among males having the LCIV near the midline or the iliac crest relatively low, a surgical corridor external to the LCIV should be taken into consideration.


Assuntos
Vértebras Lombares/diagnóstico por imagem , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Fusão Vertebral/métodos , Tomografia Computadorizada por Raios X/métodos , Adulto , Idoso , Feminino , Humanos , Vértebras Lombares/cirurgia , Masculino , Pessoa de Meia-Idade
20.
Front Surg ; 8: 602513, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34055864

RESUMO

Background: To compare the clinical efficacy of granular bone grafts and transverse process bone grafts for single-segmental thoracic tuberculosis (TB). Methods: The clinical records of 52 patients who were diagnosed with single-segmental thoracic TB and treated by one stage posterior debridement, bone graft fusion, and internal fixation in our department from 2015 to 2018 were retrospectively analyzed. Among them, 25 cases were in the granular bone graft group and 27 cases in the transverse processes bone graft group. Outcomes including the visual analog scale (VAS), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), neurological function, operative time, operative blood loss, hospital stay, Cobb angle, bone graft fusion time, and postoperative complications were all recorded and analyzed. Results: There were no significant differences in operative time, operative blood loss, and hospital stay between the two groups (P > 0.05). With an average follow-up of 18-33 months, all patients in the two groups showed significant improvement in VAS score, ESR, CRP, and neurological function compared with preoperative measurements (P < 0.05), however, no significant differences were found for the last follow-up (P > 0.05). The two groups showed similar Cobb angle correction (P > 0.05), but the granular bone graft group had a larger Cobb angle loss than the transverse processes bone graft group (P < 0.05). The bone graft fusion time of the granular bone graft group was shorter than that of the transverse processes bone graft group (P < 0.05). No significant difference was found in the postoperative complications rate between the two groups (P > 0.05). Conclusion: Granular bone grafts and transverse process bone grafts may achieve comparable clinical efficacy for single-segmental thoracic TB, but the former method had a shorter bone fusion time.

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