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1.
Hum Cell ; 33(2): 405-415, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31925702

RESUMO

Recently, miR-362-5p has attracted special interest as a novel prognostic predictor in acute myeloid leukemia (AML). However, its biological function and underlying molecular mechanism in AML remain to be further defined. Herein, we found that a significant increase in miR-362-5p expression was observed in AML patients and cell lines using quantitative real-time PCR. The expression of miR-362-5p was altered in THP-1 and HL-60 cells by transfecting with miR-362-5p mimic or inhibitor. A series of experiments showed that inhibition of miR-362-5p expression significantly suppressed cell proliferation, induced G0/G1 phase arrest and attenuated tumor growth in vivo. On the contrary, ectopic expression of miR-362-5p resulted in enhanced cell proliferation, cell cycle progression and tumor growth. Moreover, growth arrest-specific 7 (GAS7) was confirmed as a direct target gene of miR-362-5p and was negatively modulated by miR-362-5p. GAS7 overexpression imitated the tumor suppressive effect of silenced miR-362-5p on THP-1 cells. Furthermore, miR-362-5p knockdown or GAS7 overexpression obviously down-regulated the expression levels of PCNA, CDK4 and cyclin D1, but up-regulated p21 expression. Collectively, our findings demonstrate that miR-362-5p exerts oncogenic effects in AML by directly targeting GAS7, which might provide a promising therapeutic target for AML.

2.
Exp Ther Med ; 18(4): 2933-2941, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31572536

RESUMO

Traumatic soft tissue defects such as bedsores, chronic skin ulcers, limb necrosis, osteonecrosis and other ischemic orthopedic diseases are the most clinically intractable and common problems in orthopedics due to unsatisfactory conventional treatments. The present study designed poly(ethylene glycol; PEG) hydrogels with covalently binded arginylglycylaspartic acid (RGD). Endothelial progenitor cells (EPCs) were encapsulated in the modified hydrogel along with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Results demonstrated that the modified hydrogel displayed good mechanical properties appropriate for a sustained release carrier. RGD modification significantly promoted EPC biocompatibility. VEGF and bFGF encapsulation enhanced the adhesion of EPCs, promoted the production of extracellular matrix and facilitated EPC proliferation. In addition, bFGF and VEGF induced angiogenesis. The combination of growth factors and EPCs in the hydrogel displayed a strong synergy to improve biocompatibility. The present results provided a potential novel treatment approach for soft tissue defects such as bone exposure, chronic skin ulcers, bedsores, limb necrosis, osteonecrosis and other ischemic diseases.

3.
Drug Des Devel Ther ; 13: 1163-1170, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31043769

RESUMO

Purpose: Umbelliferone (Umb), a member of coumarin family, is found in many plants and is a promising molecule with potential anti-inflammatory, anti-oxidative, and anti-tumor activities. However, the effect of Umb on arthritis remains unclear. Methods: A rat model with Freund's complete adjuvant (FCA)-induced arthritis was developed and used to test the efficacy of Umb on arthritis rats. Rats were given an intragastric injection of Umb (20 and 40 mg/kg) once daily from days 21 to 28 after the administration of FCA. Hind paw volume was assessed using a volume meter. The pro-inflammatory cytokine levels and prostaglandin E2 (PEG2) level in serum and synovial fluid were detected by ELISA. HE staining was used to determine representative histological changes in joint tissues, and Western blot analysis was employed to study the effects of Umb on MAPK/NF-κB signaling pathway. Results: Our results showed that Umb suppressed the release of IL-6, IL-1ß, tumor necrosis factor-alpha, and PEG2. In addition, Umb could also dramatically ameliorate the pathological changes observed in rat joints. Based on the results of Western blot, we also observed that Umb could strikingly suppress the expression of MAPK/NF-κB pathway molecules. Conclusion: These results proved that treatment with Umb is very effective for arthritis and inhibiting the MAPK/NF-κB signaling pathway may be a potential therapeutic target for treatment of arthritis.


Assuntos
Artrite Experimental/tratamento farmacológico , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , NF-kappa B/antagonistas & inibidores , Umbeliferonas/farmacologia , Animais , Artrite Experimental/metabolismo , Citocinas/biossíntese , Citocinas/sangue , Injeções Intraventriculares , Proteínas Quinases Ativadas por Mitógeno/metabolismo , NF-kappa B/metabolismo , Ratos , Ratos Sprague-Dawley , Umbeliferonas/administração & dosagem , Umbeliferonas/química
4.
J Foot Ankle Surg ; 56(1): 15-18, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-27989338

RESUMO

Patellar subluxation is common in adolescents, and a variety of factors are related to this condition, with valgus of the knee joint an important factor. The results of many studies suggest that flatfoot can cause an abnormality of the lower limb power line. Structural abnormalities of the foot caused by the high stresses exerted by body weight can lead to structural deformity of the knee and can also cause knee valgus. Screening for foot problems can help determine the risk of patellar subluxation, and early intervention can lessen the incidence of this condition. The purpose of the present study was to investigate the effects of flatfoot on the structure and function of the knees and, especially, the risk of patellar subluxation. A total of 72 participants were recruited for this cross-sectional study. The mean age at examination was 15.4 ± 4.0 (range 9 to 22) years. The measured parameters were heel valgus angle, arch index, and quadriceps angle (Q-angle). Overall, the mean values of the heel valgus angle, arch index, and Q-angle were 5.9° ± 2.4° (range 1° to 11°), 0.33 ± 0.07 (range 0.23 to 0.46), and 19.1° ± 3.5° (range 9° to 26°), respectively. The Q-angle was directly associated with the heel valgus angle (r = 0.818, p < .001) and arch index (r = 0.655, p < .001). We found that flatfoot can affect the morphology of the knee joint and increase the risk of patellar subluxation.


Assuntos
Mau Alinhamento Ósseo/epidemiologia , Pé Chato/epidemiologia , Luxação Patelar/epidemiologia , Adolescente , Distribuição por Idade , Mau Alinhamento Ósseo/diagnóstico por imagem , Criança , Comorbidade , Estudos Transversais , Feminino , Pé Chato/diagnóstico por imagem , Humanos , Incidência , Masculino , Luxação Patelar/diagnóstico por imagem , Prognóstico , Medição de Risco , Índice de Gravidade de Doença , Distribuição por Sexo , Adulto Jovem
5.
Knee Surg Sports Traumatol Arthrosc ; 23(9): 2715-20, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24832692

RESUMO

PURPOSE: Post-traumatic contracture is a common complication after elbow trauma. If conservative therapy fails to restore adequate elbow motion, surgical release is recommended. Increase in arthroscopy knowledge and skills, as well as technological advances in the passed decade of years, has made arthroscopic arthrolysis a safe and reliable treatment for patients with a post-traumatic elbow contracture. The aim of this study was to report on the clinical outcome and improvement of ROM in post-traumatic stiff elbow treated by arthroscopic arthrolysis. METHODS: Between 2008 and 2012, 34 consecutive patients with post-traumatic stiffness were treated with arthroscopic arthrolysis. Active and passive elbow movement is encouraged the day after operation with the effective pain management. Mayo Elbow Performance Index (MEPI) and visual analogue scale were measured. RESULTS: At the final follow-up, the average arc of elbow motion increased from 48.6 ± 19.3 pre-operatively to 114.5 ± 25.7, with a mean improvement of 65.9°. The MEPI score improved from 68.2 ± 16.4 pre-operatively to 92.4 ± 21.6, with a mean improvement of 24.2 (p < 0.001). Results were good to excellent in 29 patients. CONCLUSION: Injuries are the most common cause of elbow stiffness requiring surgical release. The procedure of arthroscopic arthrolysis is a good option for the treatment of post-traumatic elbow stiffness as it restores normal elbow function. Early passive/active post-operative rehabilitation is very important.


Assuntos
Traumatismos do Braço/complicações , Contratura/cirurgia , Articulação do Cotovelo/cirurgia , Artropatias/cirurgia , Adulto , Traumatismos do Braço/diagnóstico por imagem , Traumatismos do Braço/reabilitação , Traumatismos do Braço/cirurgia , Artroscopia , Contratura/diagnóstico por imagem , Contratura/etiologia , Contratura/reabilitação , Articulação do Cotovelo/diagnóstico por imagem , Feminino , Humanos , Artropatias/diagnóstico por imagem , Artropatias/etiologia , Artropatias/reabilitação , Masculino , Pessoa de Meia-Idade , Radiografia , Amplitude de Movimento Articular , Recuperação de Função Fisiológica , Adulto Jovem
6.
J Surg Oncol ; 109(7): 714-20, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24395023

RESUMO

BACKGROUND: The current trend is toward salvage of the extremity after tumor excision without compromising the extent of resection for bone tumor around the shoulders. OBJECTIVES: The aim of this study was to evaluate functional outcome of patients treated with limb-salvage surgeries combined with shoulder abduction braces. METHODS: Thirty-six patients with bone tumors around the shoulders, who had limb-sparing resection and reconstruction performed with a shoulder abduction brace, were retrospectively reviewed. Allograft transplantation and rigid internal fixation was performed in 22 patients and artificial prosthetic replacement was performed in 14 patients. Functional evaluation was performed based on the Musculoskeletal Tumour Society (MSTS) scoring system. RESULTS: The overall survival was 78.8% (26/33) at 2 years. The mean final functional score was (81.2 ± 19.6%). The MSTS of patients treated by allograft transplantation and prosthetic replacement were (79.4 ± 15.3%) and (81.9 ± 18.1%), respectively. The MSTS scores differed only slightly between these two groups (P > 0.05). All the patients regained good ROM of the shoulder joints. CONCLUSIONS: Satisfactory functional outcomes can be obtained by limb-salvage surgery for bone tumor around the shoulder. Postoperatively shoulder crutches with shoulder abduction brace are encouraged as the aid of reconstruction of shoulder joint function.


Assuntos
Neoplasias Ósseas/cirurgia , Salvamento de Membro , Ombro/cirurgia , Adolescente , Adulto , Neoplasias Ósseas/mortalidade , Neoplasias Ósseas/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Amplitude de Movimento Articular , Procedimentos Cirúrgicos Reconstrutivos , Estudos Retrospectivos , Articulação do Ombro/fisiopatologia , Transplante Homólogo , Resultado do Tratamento
7.
Oncol Rep ; 30(5): 2211-9, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23970332

RESUMO

Mesenchymal stem cells (MSCs) are multipotent stem cells with the ability to migrate to sites of inflammation and injury, where they participate in tissue regeneration and repair. The present study aimed to investigate the effects of T cell activation and inflammation on the differentiation of MSCs. Human trabecular bone­derived MSCs were isolated from patients undergoing total hip replacement, and T cells were isolated and purified from peripheral blood mononuclear cells (PBMCs) using CD3 MicroBeads. MSCs were co­cultured with activated T cells to mimic the inflammatory microenvironment. MTS assay was used to detect cell proliferation.qRT­PCR, western blotting, histology and immunohistochemical staining were used to detect the adipo­/osteo­specific gene expression and the relative signaling pathway. The MTS results showed that higher concentrations of T cells significantly increased the proliferation of MSCs. Expression of the inflammatory gene IL­6 was upregulated, while expression of IL­10 and INFγ was downregulated in MSCs exposed to activated T cells. The results also showed that PHA­activated T cells significantly upregulated the expression of PPARγ and FABP4 (adipo­specific genes) in MSCs, but no difference was noted in the expression of RUNX2, osteocalcin and ALP (osteo­specific genes) at the protein level. T cell treatment and inflammation inhibited the protein expression of TGF­ß1 and the phosphorylation of Smad3, resulting in the weakening of the TGF­ß/Smad pathway and enhancing the adipogenic differentiation of MSCs. The results indicated that PHA­activated T cells and inflammation could promote adipogenesis without affecting the late stage of osteogenesis of MSCs, by increasing the expression of key adipogenic genes through TGF­ß/Smad3 signaling.


Assuntos
Diferenciação Celular/genética , Inflamação/genética , Células-Tronco Mesenquimais/citologia , Linfócitos T/citologia , Adipogenia/genética , Adulto , Artroplastia de Quadril , Proliferação de Células , Células Cultivadas , Microambiente Celular/genética , Humanos , Inflamação/patologia , Interferon gama/metabolismo , Interleucina-10/metabolismo , Interleucina-6/metabolismo , Leucócitos Mononucleares , Osteogênese/genética , Transdução de Sinais , Linfócitos T/metabolismo
8.
Exp Mol Pathol ; 94(1): 247-54, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22750282

RESUMO

OBJECTIVES: Osteonecrosis is also known as avascular necrosis, and two types of cell death are included in the pathogenesis of osteonecrosis: necrosis and apoptosis. Apoptosis in the osteonecrosis of femoral head is thought to be the key determinant of glucocorticoid-induced cortical bone loss. The present study was implemented to evaluate the anti-apoptotic effect of Granulocyte colony-stimulating factor and stem cell factor (G-CSF/SCF) in rabbits with steroid-induced osteonecrosis. METHODS: In the experiment, osteonecrosis was induced by low-dose lipopolysaccharide and subsequent pulsed high-dose methylprednisolone. Rabbits in preventive medicine group were treated with 100 µg/kg/d G-CSF and 25 µg/kg/d SCF. Then hematological and histomorphometric methods were used to investigate the treatment effects of osteonecrosis. Apoptosis was assessed via quantitative TUNEL staining and activated caspase-3 immunostaining and immunoblotting. RESULTS: The results showed that G-CSF/SCF treatment could increase the secretion of serum osteocalcin, but inhibit the expression of serum tartrate-resistant acid phosphatase (TRAP5b). The incidence of osteonecrosis was significantly decreased in Preventive group when compared with Steroid group (42.1% vs. 88.2%). Histomorphometric analysis showed that G-CSF/SCF pre-disposal treatment was able to increase trabecular mineral appositional rate (MAR) and bone formation rate (BFR). Quantitative TUNEL and activated caspase-3 levels showed lower apoptosis in the Preventive group. CONCLUSIONS: In conclusion, G-CSF/SCF treatment could inhibit caspase-3-dependent apoptosis in osteocytes to exert beneficial effects in preventing steroid-induced ON in rabbit models.


Assuntos
Apoptose/efeitos dos fármacos , Fator Estimulador de Colônias de Granulócitos/farmacologia , Osteonecrose/tratamento farmacológico , Osteonecrose/prevenção & controle , Fator de Células-Tronco/farmacologia , Fosfatase Ácida/sangue , Animais , Caspase 3/sangue , Fator Estimulador de Colônias de Granulócitos/uso terapêutico , Isoenzimas/sangue , Metilprednisolona , Osteocalcina/sangue , Osteogênese/efeitos dos fármacos , Osteonecrose/induzido quimicamente , Coelhos , Fator de Células-Tronco/uso terapêutico , Fosfatase Ácida Resistente a Tartarato
9.
J Huazhong Univ Sci Technolog Med Sci ; 30(4): 477-81, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20714873

RESUMO

The mobilization efficiency of granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) to bone marrow mononuclear cells (MNCs) in mice was observed, and the changes of CXCL12/CXCR4 signal were detected in order to find out the mobilization mechanism of stem cells. Kunming mice were randomly divided into two groups. The mice in treatment group were subjected to subcutaneous injection of G-CSF at a dose of 100 microg/kg and SCF at a dose of 25 microg/kg every day for 5 days, and those in control group were given isodose physiological saline. The MNCs were separated, counted and cultured, and the colony-forming unit-fibroblast (CFU-F) was evaluated. CD34+CXCR4+ MNCs were sorted by flow cytometry. The expression of CXCL12 protein in bone marrow extracellular fluid was detected by ELISA, and that of CXCL12 mRNA in bone marrow was measured by RT-PCR. The results showed that the counts of MNCs in peripheral blood and bone marrow were increased after administration of G-CSF/SCF (P<0.01). The factors had a dramatic effect on the expansion capability of CFU-F (P<0.05). Flow cytometric of bone marrow MNCs surface markers revealed that CD34+CXCR4+ cells accounted for 44.6%+/-8.7% of the total CD34+ MNCs. Moreover, G-CSF/SCF treatment induced a decrease in bone marrow CXCL12 mRNA that closely mirrored the fall in CXCL12 protein. In this study, it is evidenced that G-CSF/SCF can effectively induce MNCs mobilization by disrupting the balance of CXCL12/CXCR4 signaling pathway in the bone marrow and down-regulating the interaction of CXCL12/CXCR4.


Assuntos
Células da Medula Óssea/citologia , Movimento Celular/efeitos dos fármacos , Fator Estimulador de Colônias de Granulócitos/farmacologia , Leucócitos Mononucleares/citologia , Fator de Células-Tronco/farmacologia , Animais , Células Cultivadas , Quimiocina CXCL12/metabolismo , Regulação para Baixo/efeitos dos fármacos , Camundongos , Receptores CXCR4/metabolismo , Transdução de Sinais
10.
Artigo em Chinês | MEDLINE | ID: mdl-19514591

RESUMO

OBJECTIVE: To study the operative procedure and effect of arthroscopic reconstruction of both anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) with anterior tibialis tendon allograft. METHODS: From February 2005 to July 2006, 10 cases of both ACL and PCL rupture were reconstructed with anterior tibialis tendon allograft, including 7 men and 3 women, aging 18-45 years with an average of 30.2 years. The locations were left knee in 6 cases and right knee in 4 cases. All of them had identified trauma history. The disease course was about 1-3 weeks (mean 1.8 weeks). Both ACL and PCL were reconstructed under arthroscope with allograft anterior tibialis tendon of 26-28 cm in length and immobilization with extention position brace was given for 4 weeks after operation. The active flex knee exercise was done from 0-90 degrees at 4 weeks and more than 90 degrees at 6 weeks. RESULTS: All operations were finished successfully, there were no blood vessel and nerve injury. The operative time was 90-110 minutes (mean 100 minutes). The wound healed by first intention and no early complication occurred. Ten cases were followed up for 12 months to 15 months with an average of 13.5 months. Thier gait was normal, knee activity degree was 0-135 degrees. The anterior drawing tests and media and lateral stress tests were negative after operation in 10 cases; and the posterior drawing tests were negative in 8 cases and 2 cases was at grade I. Hydra arthrosis of knee occurred in 2 cases and was cured after remove of fluid and injection of sodium hyaluronate. The Lysholm knee function score was increases from 24.89 +/- 5.39 before operation to 96.00 +/- 4.59 at 12 months after operation, showing significant difference (P < 0.05). CONCLUSION: Arthroscopic reconstruction of both ACL and PCL with anterior tibialis tendon allograft has the advantages of short operation time, less complications and good clinical effects.


Assuntos
Ligamento Cruzado Anterior/cirurgia , Ligamento Cruzado Posterior/cirurgia , Tendões/transplante , Adolescente , Adulto , Lesões do Ligamento Cruzado Anterior , Artroscopia , Feminino , Humanos , Traumatismos do Joelho/cirurgia , Articulação do Joelho/cirurgia , Masculino , Pessoa de Meia-Idade , Ligamento Cruzado Posterior/lesões , Procedimentos Cirúrgicos Reconstrutivos/métodos , Resultado do Tratamento , Adulto Jovem
11.
Artigo em Inglês | MEDLINE | ID: mdl-19224169

RESUMO

In order to investigate the effect of Arg-Gly-Asp (RGD) peptide-modified silk biomaterial on the adhesion and proliferation of bone marrow-derived mesenchymal stem cells (MSCs), MSCs of third generation were seeded onto the surface of RGD-decorated silk (silk-RGD group), silk alone (silk group) or tissue culture plate (TCP group). After incubation for 4 or 12 h, MSCs were examined quantitatively by using precipitation method for cell attachment. The cell proliferation, which was defined as cell density, was compared among the three groups after culture for 1, 2, 3, and 4 days. Cell skeleton, which was labeled fluorescently, was observed under laser confocal microscope after 24 h of culture. The results showed that cell adhesion rate in silk-RGD group was higher than in silk group (P<0.05), but similar to that in TCP group after incubation for 4 or 12 h (P>0.05). There were no significant differences in the cell proliferation among the three groups at different time points (P>0.05 for all). Laser confocal microscopy revealed that in silk-RGD group, MSCs, strongly fluorescently stained, spread fully, with stress fibers clearly seen, while in silk group, actin filaments were sparsely aligned and less stress fibers were found. It was concluded that RGD peptide could improve the adhesion of MSCs to the silk scaffold, but had no impact on the proliferation of the cells.


Assuntos
Materiais Biocompatíveis/química , Células-Tronco Mesenquimais/citologia , Oligopeptídeos/química , Seda , Tecidos Suporte , Células da Medula Óssea/citologia , Adesão Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Humanos , Seda/química
12.
J Rheumatol ; 35(11): 2241-8, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18792998

RESUMO

OBJECTIVE: Bone marrow-derived stem cells (BMSC) have been highlighted for the treatment of osteonecrosis (ON) before collapse of the femoral head. In our study, the potential of granulocyte colony-stimulating factor/stem cell factor (G-CSF/SCF)-mobilized BMSC to repair steroid-associated ON was assessed in rabbits. METHODS: ON was induced by low-dose lipopolysaccharide and subsequent pulsed high-dose methylprednisolone. Rabbits in the treated group were subjected to subcutaneous injections of G-CSF at a dose of 100 microg/kg and SCF 25 microg/kg per day for 5 days; rabbits in the control group were given saline. Blood samples were collected and serum osteocalcin was detected by ELISA. Radiological analysis was performed by magnetic resonance imaging (MRI). Then bilateral femora and humeri were harvested and processed to paraffin sections and hard-tissue sections for immunohistochemical, histologic, and histomorphometric analysis. RESULTS: . The mean number of leukocytes and relative numbers of mononuclear cells increased significantly after mobilization. All rabbits displayed a marked increase in osteocalcin protein expression in response to G-CSF/SCF. MRI scans showed a reactive interface between the necrotic and reparative zones after G-CSF/SCF administration. Quantitative analysis showed that new vessel formation was 3.3-fold greater and vessel density was 2.6-fold greater in the treatment group than the control group. The histologic and histomorphometric analysis revealed that the new bone volume was significantly higher in the G-SCF/SCF group than in the control group at 4 weeks. CONCLUSION: G-CSF/SCF-induced mobilization of BMSC in the necrotic foci may represent a promising strategy for promoting functional bone repair of early-stage ON.


Assuntos
Glucocorticoides/farmacologia , Fator Estimulador de Colônias de Granulócitos/farmacologia , Metilprednisolona/farmacologia , Osteonecrose/induzido quimicamente , Osteonecrose/tratamento farmacológico , Fator de Células-Tronco/farmacologia , Animais , Quimioterapia Combinada , Mobilização de Células-Tronco Hematopoéticas , Células-Tronco Hematopoéticas/citologia , Lipopolissacarídeos/farmacologia , Imagem por Ressonância Magnética , Masculino , Necrose , Osteonecrose/patologia , Coelhos
13.
J Integr Plant Biol ; 50(3): 257-64, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-18713357

RESUMO

Plant water sources were estimated by two or three compartment linear mixing models using hydrogen and oxygen isotope (deltaD and delta18O) values of different components such as plant xylem water, precipitation and river water as well as soil water on the Tibetan Plateau in the summer of 2005. Four dominant species (Quercus aquifolioides, Pinus tabulaeformis, Salix rehderiana and Nitraria tangutorum) in three typical ecosystems (forest, shrub and desert) were investigated in this study. Stable isotope ratios of the summer precipitations and the soil water presented variations in spatial and temporal scales. delta18O values of N. tangutorum xylem water were constant in the whole growth season and very similar to those of deep soil water. Water sources for all of the plants came from both precipitations and soil water. Plants switched rapidly among different water sources when environmental water conditions changed. Rainwater had different contributions to the plants, which was influenced by amounts of precipitation. The percentage of plant xylem water derived from rainwater rose with an increase in precipitation. Water sources for broad-leaved and coniferous species were different although they grew in the same environmental conditions. For example, the broad-leaved species Q. aquifolioides used mainly the water from deep soil, while 92.5% of xylem water of the coniferous species P. tabulaeformis was derived from rainwater during the growth season. The study will be helpful for us to fully understand responses of species on the Tibetan Plateau to changes in precipitation patterns, and to assess accurately changes of vegetation distribution in the future.


Assuntos
Ecossistema , Árvores/fisiologia , Água/fisiologia , China , Clima , Geografia , Isótopos de Oxigênio , Pinus/crescimento & desenvolvimento , Quercus/crescimento & desenvolvimento , Chuva , Salix/crescimento & desenvolvimento , Estações do Ano , Solo , Árvores/crescimento & desenvolvimento , Xilema/fisiologia
14.
Joint Bone Spine ; 75(5): 573-8, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18468472

RESUMO

OBJECTIVES: The pathogenetic mechanisms involved in steroid-induced osteonecrosis are poorly understood. Appropriate experimental models of the human disease are indispensable to the understanding of successful treatment modalities for osteonecrosis. METHODS: In the present experiment we devised a novel rabbit model of steroid-induced osteonecrosis by use of two low-dose LPS and three high-dose MPS to investigate the development of osteonecrosis. Thirty eight rabbits were used and tissue assessments were performed on proximal third and distal condyles of femora and humeri obtained 6 weeks after the administration of LPS and MPS. MRI of these regions and intraosseous pressure of proximal femur were obtained at 0 and 6 weeks. Other assessments included serum plasminogen activator/inhibitor ratio, cholesterol level, LDL/HDL ratio, and triglyceride levels at various time points. RESULTS: The study showed that with this osteonecrosis induction protocol there was low animal mortality (6.2%), high rate of osteonecrosis (90%), induction of thrombotic state, and hypercholesterol/lipidemia. DISCUSSION: On the whole, this is a novel modified animal model of steroid associated osteonecrosis and it would be useful for elucidating the pathogenesis of steroid associated osteonecrosis and developing preventive and therapeutic strategies.


Assuntos
Modelos Animais de Doenças , Osteonecrose/patologia , Adipócitos/efeitos dos fármacos , Adipócitos/patologia , Animais , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/patologia , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Fêmur/efeitos dos fármacos , Fêmur/patologia , Glucocorticoides/toxicidade , Úmero/efeitos dos fármacos , Úmero/patologia , Lipídeos/sangue , Lipopolissacarídeos/toxicidade , Longevidade/efeitos dos fármacos , Imagem por Ressonância Magnética , Masculino , Metilprednisolona/toxicidade , Osteonecrose/sangue , Osteonecrose/induzido quimicamente , Inibidor 1 de Ativador de Plasminogênio/sangue , Pressão , Coelhos , Ativador de Plasminogênio Tecidual/sangue
15.
J Neurotrauma ; 24(9): 1502-12, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17892411

RESUMO

Recent studies confirmed that the new cell survival signal pathway of Insulin-PI3K-Akt exerted cyto-protective actions involving anti-apoptosis. This study was undertaken to investigate the potential neuroprotective effects of insulin in the pathogenesis of spinal cord injury (SCI) and evaluate its therapeutic effects in adult rats. SCI was produced by extradural compression using modified Allen's stall with damage energy of 40 g-cm force. One group of rats was subjected to SCI in combination with the administration of recombinant human insulin dissolved in 50% glucose solution at the dose of 1 IU/kg day, for 7 days. At the same time, another group of rats was subjected to SCI in combination with the administration of an equal volume of sterile saline solution. Functional recovery was evaluated using open-field walking, inclined plane tests, and motor evoked potentials (MEPs) during the first 14 days post-trauma. Levels of protein for B-cell lymphoma/leukemia-2 gene (Bcl-2), Caspase-3, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were quantified in the injured spinal cord by Western blot analysis. Neuronal apoptosis was detected by TUNEL, and spinal cord blood flow (SCBF) was measured by laser-Doppler flowmetry (LDF). Ultimately, the data established the effectiveness of insulin treatment in improving neurologic recovery, increasing the expression of anti-apoptotic bcl-2 proteins, inhibiting caspase-3 expression decreasing neuronal apoptosis, reducing the expression of proinflammatory cytokines iNOS and COX-2, and ameliorating microcirculation of injured spinal cord after moderate contusive SCI in rats. In sum, this study reported the beneficial effects of insulin in the treatment of SCI, with the suggestion that insulin should be considered as a potential therapeutic agent.


Assuntos
Apoptose/efeitos dos fármacos , Insulina/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Recuperação de Função Fisiológica/efeitos dos fármacos , Traumatismos da Medula Espinal/tratamento farmacológico , Animais , Western Blotting , Expressão Gênica/efeitos dos fármacos , Humanos , Marcação In Situ das Extremidades Cortadas , Fluxometria por Laser-Doppler , Masculino , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes/uso terapêutico , Traumatismos da Medula Espinal/patologia
16.
J Huazhong Univ Sci Technolog Med Sci ; 27(1): 68-71, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17393114

RESUMO

The protective effect of niacinamide on interleukin-1beta (IL-1beta)-induced annulus fibrosus (AF) type II collagen degeneration in vitro and the mechanism were investigated. Chiba's intervertebral disc (IVD) culture models in rabbits were established and 48 IVDs from 12 adult Japanese white rabbits were randomly divided into 4 groups: normal control group, niacinamide-treated group, type II collagen degneration group (IL-1beta) and treatment group (niacinamide+IL-1beta). After culture for one week, AFs were collected for inducible nitric oxide synthase (iNOS), cysteine containing aspartate specific protease-3 (Caspase-3) and type II collagen immunohistochemical examination, and type II collagen reverse transcription polymerase chain reaction (RT-PCR). The results showed that rate of iNOS positive staining AF cells in the 4 groups was 17.6%, 10.9%, 73.9% and 19.3% respectively. The positive rate in treatment group was significantly lower than in the type II collagen degeneration group (P<0.01). Rate of Caspase-3 positive staining AF cells in the 4 groups was 3.4%, 4.2%, 17.6% and 10.3% respectively. The positive rate in treatment group was lower than in the type II collagen degeneration group (P<0.01). Type II collagen staining demonstrated that lamellar structure and continuity of collagen in treatment group was better reversed than in the degeneration group. RT-PCR revealed that the expression of type II collagen in treatment group was significantly stronger than that in type II collagen degeneration group (P<0.01). It was concluded that niacinamide could effectively inhibit IL-1beta stimulated increase of iNOS and Caspase-3 in AF, and alleviate IL-1beta-caused destruction and synthesis inhibition of type II collagen. Niacinamide is of potential for clinical treatment of IVD degeneration.


Assuntos
Colágeno Tipo II/metabolismo , Disco Intervertebral/efeitos dos fármacos , Disco Intervertebral/metabolismo , Niacinamida/farmacologia , Substâncias Protetoras/farmacologia , Animais , Caspase 3/metabolismo , Células Cultivadas , Colágeno Tipo II/genética , Imuno-Histoquímica , Técnicas In Vitro , Interleucina-1/farmacologia , Disco Intervertebral/citologia , Disco Intervertebral/patologia , Óxido Nítrico Sintase Tipo II/biossíntese , Óxido Nítrico Sintase Tipo II/genética , Coelhos , Distribuição Aleatória
17.
Artigo em Inglês | MEDLINE | ID: mdl-16961281

RESUMO

To discuss and evaluate the method and effect of using calcaneal anatomic plate in treatment of intra-articular fractures of the calcaneus with assistant of arthroscope, 86 intra-articular fractures of the calcaneus in 78 patients were reduced by open reduction, and rigid fixation was made with calcaneal anatomic plate under assistant of arthroscope. The average follow-up duration was 18 months (range 12-30 months). The effect of treatment was evaluated according to AOFAS and X-ray before and after operation. The results showed that 86 patients have obtained satisfactory reduction according to X-ray, and there was significant difference before and after operation (P < 0. 01), the total excellent and fine rate was 91.86%. Treating intra-articular fractures of the calcaneus with calcaneal anatomic plate under arthroscope may provide more chance to achieve anatomical reconstruction, which can lead to satisfied recovery of function and few complication.


Assuntos
Placas Ósseas , Calcâneo/lesões , Fixação Interna de Fraturas/métodos , Fraturas Ósseas/cirurgia , Adulto , Artroscopia , Calcâneo/cirurgia , Feminino , Fixação Interna de Fraturas/instrumentação , Humanos , Masculino , Pessoa de Meia-Idade , Articulação Talocalcânea , Resultado do Tratamento
18.
Chin Med Sci J ; 21(2): 107-10, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16845798

RESUMO

OBJECTIVE: To investigate the effect of interleukin-6 (IL-6) on the apoptosis of annulus fibrosus (AF) cell induced by interleukin-1beta (IL-1beta). METHODS: Cultured AF cells were divided into 6 groups and treated with no drug, 10 ng/mL IL-6, 10 ng/mL IL-1beta, 10 ng/mL IL-1beta and Z-VAD-FMK (a caspase-9 inhibitor), 10 ng/mL IL-1beta and 10 ng/mL IL-6, 10 ng/mL IL-1beta and 100 ng/mL IL-6, respectively. After three days of culture, the apoptosis rate, the positive rates of caspase-3, -8, and -9 of AF cells were detected with flow cytometry. RESULTS: The apoptosis rates of cells in group 1 to 6 were 2.67% +/- 1.08%, 2.71% +/- 0.53%, 20.37% +/- 1.57%, 11.34% +/- 0.67%, 18.17% +/- 0.74%, and 9.42% +/- 1.08%, respectively. There was no significant difference between group 1 and 2, while the apoptosis rates of group 4, 5, and 6 were significantly lower than group 3 (P = 0.001, P = 0.172, and P = 0.001, respectively). Positive rates of caspase-3 in group 5 (12.35% +/- 0.64%) and 6 (9.26% +/- 0.36%) were significantly lower than group 3 (17.14% +/- 0.72%; P = 0.001 and P < 0.001, respectively). And positive rates of caspase-9 in group 5 (15.13% +/- 1.45%) and 6 (10.17% +/- 2.50%) were significantly lower than group 3 (19.4% +/- 0.98% ; P = 0.014 and P = 0.004, respectively). But there was not obvious change of caspase-8 activity after IL-6 was added. CONCLUSION: IL-6 is capable of protecting AF cells from IL-1beta induced apoptosis in vitro. Mechanism of the protection is related with the inhibition of caspase-3 and -9 activities.


Assuntos
Apoptose/efeitos dos fármacos , Interleucina-1beta/farmacologia , Interleucina-6/farmacologia , Disco Intervertebral/citologia , Disco Intervertebral/efeitos dos fármacos , Clorometilcetonas de Aminoácidos/farmacologia , Animais , Inibidores de Caspase , Células Cultivadas , Inibidores de Cisteína Proteinase/farmacologia , Disco Intervertebral/enzimologia , Coelhos
19.
Artigo em Inglês | MEDLINE | ID: mdl-16718929

RESUMO

The regulatory effects of niacinamide (Nia) on intervertebral disc (IVD) aggrecan in vitro was investigated. Chiba's 10 ng/mL interleukin-1 (IL-1)-induced rabbit IVD degeneration model in vitro was established. 0.5, 0.25 and 0.05 mg/mL Nia was added to normal and degenerated IVDs for intervention. On the first and second week after intervention, safranin O-fast green staining intensity and glycosaminoglycan (GS) content were measured. The expression of aggrecan core protein was detected by RT-PCR. The results showed: (1) After treatment with 0.5 mg/mL Nia for one week, the GS content in nucleus pulposus (NP) was increased by 44.8% as compared with control group (P < 0 01); The GS content in IL-1 induction groups was increased with the increase of Nia concentrations: After treatment with 0.5 mg/mL for one week, the GS content in NP was increased by 68.3% as compared with control group (P < 0.01). After two weeks, GS content in NP and fibrous rings was still higher than in control group at the same period (P < 0.01) and untreated group (P < 0.01). (2) Safranin O-fast green staining revealed that with the increase of Nia concentrations, staining density in NP and fibrous rings was increased and histological structure damage to IVDs by IL-1beta was alleviated. (3) RT-PCR showed that the expression of core protein gene in IL-1beta-induced degenerated IVDS was increased with the increase of Nia concentrations. It was concluded that under conditions in vitro, Nia could up-regulate the expression of aggrecan in IVDs and protect IVDs from IL-1beta-induced degeneration at least partially, which offers a potential choice for IVD degeneration clinical therapy.


Assuntos
Proteoglicanas de Sulfatos de Condroitina/biossíntese , Proteínas da Matriz Extracelular/biossíntese , Disco Intervertebral/metabolismo , Lectinas Tipo C/biossíntese , Niacinamida/farmacologia , Agrecanas , Animais , Células Cultivadas , Proteoglicanas de Sulfatos de Condroitina/genética , Proteínas da Matriz Extracelular/genética , Glicosaminoglicanos/biossíntese , Glicosaminoglicanos/genética , Interleucina-1/farmacologia , Disco Intervertebral/citologia , Disco Intervertebral/patologia , Lectinas Tipo C/genética , Coelhos , Regulação para Cima
20.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 20(3): 443-6, 2003 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-14565009

RESUMO

To investigate the effect of basic fibroblast growth factor(bFGF) gene transfection on the proliferation and differentiation of mesenchymal stem cells (MSCs) and to provide basis for accelerating bone defect repairing using gene-enhanced tissue engineering technology, Rabbit periosteum-derived MSCs were transfected with the full-length rat bFGF cDNA in vitro. The transient and stable gene expression of bFGF were determined by immunohistochemistry. The proliferation and the synthesis alkaline phosphatase (ALP) and osteocalcin(OC) of the transfected MSCs were also examined. The results showed that bFGF cDNA could be transferred into osteoblasts and expressed stably at least 4 weeks. The proliferation and OC content of genetically modified MSCs were increased significantly, whereas the ALP activity remained no change. In conclusion, transfer of gene encoding bFGF to MSCs increases its proliferation and osteogenesis property. Based on the successful conjunction of the existing techniques of tissue engineering with the novel possibilities offered by modern gene transfer technology, an innovative concept, molecular tissue engineering, was put forward for the first time. As a new branch of tissue engineering, it represents both a new area and an important trend in tissue engineering research.


Assuntos
Fator 2 de Crescimento de Fibroblastos/fisiologia , Células-Tronco/citologia , Células Estromais/citologia , Engenharia Tecidual/métodos , Fosfatase Alcalina/biossíntese , Animais , Diferenciação Celular/fisiologia , Divisão Celular/fisiologia , Células Cultivadas , Fator 2 de Crescimento de Fibroblastos/genética , Osteocalcina/biossíntese , Coelhos , Células-Tronco/fisiologia , Células Estromais/fisiologia , Transfecção
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