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1.
Food Chem ; 328: 127112, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32470778

RESUMO

A ratiometric fluorescent probe (Probe 1) was developed for the sensitive detection of ß-galactosidase (ß-gal) activity. Probe 1 detected ß-gal activity in the range 0-1.0 U/mL, with a limit of detection of 0.025 U/mL. In addition, as different activities of ß-gal added, the luminescent intensity of Probe 1 gradually increased, as observed under a 365 nm ultraviolet lamp. Moreover, this method is low-volume, 20 µL, and time-efficient, 45 min per measurement. Probe 1 was successfully used to measure the ß-gal activity in real fruit samples in a qualitative manner, by the naked eye, fast semi-quantitative manner, by smartphone, or quantitative manner, by fluorescence spectrometer.

2.
Mol Cells ; 43(6): 517-529, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32434298

RESUMO

Carboxyl-terminal binding proteins (CtBPs) are transcription regulators that control gene expression in multiple cellular processes. Our recent findings indicated that overexpression of CtBP2 caused the repression of multiple bone development and differentiation genes, resulting in atrophic nonunion. Therefore, disrupting the CtBP2-associated transcriptional complex with small molecules may be an effective strategy to prevent nonunion. In the present study, we developed an in vitro screening system in yeast cells to identify small molecules capable of disrupting the CtBP2-p300 interaction. Herein, we focus our studies on revealing the in vitro and in vivo effects of a small molecule NSM00158, which showed the strongest inhibition of the CtBP2-p300 interaction in vitro. Our results indicated that NSM00158 could specifically disrupt CtBP2 function and cause the disassociation of the CtBP2-p300-Runx2 complex. The impairment of this complex led to failed binding of Runx2 to its downstream targets, causing their upregulation. Using a mouse fracture model, we evaluated the in vivo effect of NSM00158 on preventing nonunion. Consistent with the in vitro results, the NSM00158 treatment resulted in the upregulation of Runx2 downstream targets. Importantly, we found that the administration of NSM00158 could prevent the occurrence of nonunion. Our results suggest that NSM00158 represents a new potential compound to prevent the occurrence of nonunion by disrupting CtBP2 function and impairing the assembly of the CtBP2-p300-Runx2 transcriptional complex.

3.
Dig Surg ; 37(3): 211-219, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31269486

RESUMO

BACKGROUND: Percutaneous or endoscopic drainage was usually performed as the first step in step-up approach for infected pancreatic necrosis (IPN). However, drainage was unnecessary or unavailable in some patients. OBJECTIVE: To estimate the safety and effect of one-step laparoscopic-assisted necrosectomy in IPN patients. METHODS: A retrospective analysis of IPN patients received surgical therapy in our center between January 2015 and December 2017 was performed. Patients were assigned to either one-step or step-up groups according to the received therapeutic approach. Incidence of complications, death, total number of interventions, and total hospital stay were compared. Logistic regression and nomogram were used to explore the risk factors and probability for patients undergoing interventions ≥3 times. RESULTS: There were 45 and 49 patients included in one-step and step-up groups, respectively. No significant difference between groups in terms of new organ failure (14.29 vs. 14.33%, p = 0.832), death (8.89 vs.8.17%, p = 0.949), and long-term complications (18.37 vs. 15.56%, p = 0.717). However, the number of interventions in one-step group was significantly less than in step-up group with shorter hospital stay. After multivariate analysis, C-reactive protein, interleukin-6, and surgical approach were independent predicators for patients undergoing interventions ≥3. A nomogram was built with area under ROC curve 0.891. CONCLUSION: Compared with step-up approach, one-step surgery was safe and effective in selected IPN patients with less interventions and shorter hospital stay.

4.
Materials (Basel) ; 12(20)2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31623236

RESUMO

The fatigue life of carbon fiber reinforced polymer (CFRP) tendons was studied in this paper. A new wedge-type anchorage system was applied to the fatigue test of CFRP tendons and demonstrated an excellent fatigue resistance. In the test and analytical data, the fatigue stress ranged from 200 MPa to 800 MPa, and maximum stresses from 0.37 to 1.0 fu (fu = ultimate tensile strength of CFRP tendons) were determined. The main work and results were that the stress range and stress level (maximum stress) were two key parameters affecting the fatigue life of CFRP tendons. A bilinear equation and a linear equation considering the fatigue life of CFRP tendons jointly affected by the stress range and the maximum stress were established. The error of predicted results and test results was 0.038 and 0.083, respectively, both representing good prediction accuracy. The predicted results of Whitney's method showed that, at a 95% confidence level, when the stress range was 200 MPa, 400 MPa, and 600 MPa, the maximum stress limit of CFRP tendons, which were not broken in a fatigue test of 2 million times, was 63.9% fu, 53.0% fu, and 36.8% fu, respectively.

5.
Infect Drug Resist ; 12: 2495-2503, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31616166

RESUMO

Purpose: Community-associated Staphylococcus aureus (CA S. aureus) is the most common causative pathogen of the skin and soft tissue infections (SSTIs). This study aims to determine clonal distribution, virulence factors of CA S. aureus clinical isolates from purulent SSTIs in Beijing, China. Materials and methods: CA-S. aureus isolates were collected from 115 outpatients with purulent SSTIs from the department of dermatology from April 2015 to April 2017. Multilocus sequence typing and Staphylococcus cassette chromosome mec typing were performed to explore molecular characteristics. Phylogenetic analysis of 16S rRNA of dominant S. aureus isolates was performed using MEGA-X software. Virulence genes were detected by PCR, while biofilm formation was evaluated by a microtiter plate method. The antimicrobial susceptibility was tested by an automatic VITEK system. Results: Forty-four CA-S. aureus isolates identified from SSTIs contain 9 methicillin-resistant S. aureus (MRSA) isolates (20.4%) and 35 methicillin-susceptible S. aureus isolates (MSSA) (79.6%). The dominant sequence types (STs) were ST22 (40.9%) and clonal complex 59 (CC59; 77.8%) in Community-associated methicillin resistant methicillin-resistant S. aureus. 27.8% of ST22 isolates were homologous to the epidemic ST22 EMRSA-15 in Europe. The prevalence of virulence genes lukS/lukF, tst-1, etA, edinA, icaA, and icaD was 50%, 93.2%, 4.5%, 4.5%, 100%, and 100%, respectively. All CC59 isolates exhibited stronger biofilm-forming capability than ST22 clones. Among the MSSA subgroup, the poor biofilm producers had significantly higher sensitivity to sulfamethoxazole/Trimethoprim. Conclusion: The dominant epidemic clone PVL+ ST22 MSSA containing tst-1 occurs in Beijing, indicating that a PVL+ ST398 clone which was previously predominant in this district had been replaced by a new clone.

6.
Clin Transplant ; 33(10): e13659, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31278776

RESUMO

Transplant surgical workforce concerns have arisen in the last 5 years as reflected in challenges securing job opportunities for new fellows. The present survey was designed by the ASTS Membership and Workforce Committee to describe the current practice characteristics of transplant centers in order to estimate changes in the workforce. The survey questionnaire requested information about the transplant programs, the transplant surgeons involved in the program, and the estimated changes in the staffing of the program over the next 3 years. Seventy-one transplant centers responded from a total of 235 identified and queried (30.2% response rate), with median responding centers per UNOS region of 7 (IQR 4.5-8.5). The recruitment outlook for the next 3 years forecasts a positive inflow of surgeons at a 2:1 rate (incoming:leaving). The new female transplant workforce within the responding cohort has increased from 3.7% in 1980 to 18.4% in 2010. Currently, 13.1% of practicing US transplant surgeons in this survey are female which is higher than many other surgical specialties. This report represents the most up-to-date view into the abdominal transplant surgical workforce. The positive job recruitment outlook for transplant surgeons and the narrowing gender gap are new findings from this study.

7.
Chin Med J (Engl) ; 132(13): 1550-1562, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31268882

RESUMO

BACKGROUND: Management of tumors has become more complex owing to tumor heterogeneity. Fewer studies have been performed on intra-tumor heterogeneity of endometrial cancer (EC) until now. Therefore, it is of great clinical value to explore the intra-tumor heterogeneity of EC based on clinical features and gene expression profiles. METHODS: A total of 1688 patients with EC were screened and 114 patients were finally selected, including specimens from 84 patients with primary EC without relapse (PE) and the paired metastases (P-M) specimens, as well as specimens from 30 patients with primary EC with relapse (RPE) and the paired relapsed EC (P-RE) specimens. Microarray and RNA-seq were used to detect gene expression of EC samples. Clinicopathological characteristics and molecular data were compared between PE and P-M groups and between RPE and P-RE groups to explore the intra-tumor heterogeneity of EC. RESULTS: The clinical intra-tumor spatial heterogeneity of pathological type, grade, ER status, and PR status between PE and P-M were 17.9%, 13.1%, 28.6%, and 28.6%, respectively. The clinical intra-tumor spatiotemporal heterogeneity of pathological type, grade, ER status, and PR status between RPE and P-RE were 16.7%, 33.3%, 25.0%, and 37.5%, respectively. Cluster analysis sorts EC samples based on progression type of lesion and their pathological type. There were differentially expressed genes between PE and P-M and between RPE and P-RE, of which gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis were mainly enriched in cell proliferation, the p53 signaling pathway, etc. CONCLUSIONS:: Clinical and molecular data showed that there was spatiotemporal heterogeneity in intra-tumor of EC, which may add to the complexity of diagnosis and therapeutics for EC. Considering the intra-tumor heterogeneity, sequential chemotherapy and precision medicine may be a more suitable treatment plan for EC.


Assuntos
Neoplasias do Endométrio/metabolismo , Neoplasias do Endométrio/patologia , Adulto , Idoso , Proliferação de Células/genética , Proliferação de Células/fisiologia , Análise por Conglomerados , Feminino , Humanos , Análise em Microsséries , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Proteína Supressora de Tumor p53/metabolismo , Adulto Jovem
8.
Environ Int ; 130: 104930, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31228784

RESUMO

The effects and mechanism of high-temperature annealing, a frequently-used strategy to modulate the properties of nanoparticles (NPs), on the dissolution of zinc oxide (ZnO) NPs are investigated in this study. The results show that annealing increases the ZnO NPs dissolution magnitude via increasing O vacancy abundance on the surface and in the bulk crystal. The face-dependent distribution of O vacancy is revealed by characterizing ZnO single crystal, and the (000-1) face has a higher abundance than the (10-10) face. Particularly, O vacancy abundance in the bulk (000-1) is about 3 times higher than in the bulk (10-10). Annealing further strengthens the face-dependence of O vacancy distribution, therefore both raw and annealed (000-1) faces contribute dominantly to the dissolution of ZnO NPs. Typical topographies of the surface defect sites on the (000-1) face and their evolutions during dissolution are collected. Annealing promotes the formation of larger and deeper etching pits. Elevated solution temperature and annealing synergize to further accelerate ZnO dissolution. The dissolution behaviors of ZnO NPs with different annealing statuses, surface properties, and solution temperatures investigated in this study have potential implications to the evaluations of environmental fate and risk of metal oxide NPs.


Assuntos
Nanopartículas Metálicas/química , Temperatura , Poluentes Químicos da Água/química , Óxido de Zinco/química , Solubilidade , Água
9.
Sci Total Environ ; 651(Pt 2): 2208-2217, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30326453

RESUMO

Mineral dissolution is a spontaneous process that takes indispensible role in the determination of water quality in a specific water body. Deep insights into defects as a result of characterization technique development have greatly improved our understanding of their significances and behaviors in the dissolution within the mineral-water interface. Based on the progresses from previous decades, this review attempts to re-elaborate the molecular-scale process of dissolution. Material flow within the mineral/water interface is updated, with emphasis on the function of defect sites. A brief introduction of defect properties is presented, including the microscopic appearances and typical physicochemical characteristics. Feasible strategies that have been adopted to increase the defect abundance are inferred, which maybe enlightening for hydrometallurgy. The merits and drawbacks of the techniques that could be employed for the qualitative and quantitative determination of defect presence are introduced, although relatively satisfactory performances are noted. With the aid of these techniques, it is concluded that screw dislocation is the main defect type responsible for surface topography evolution as a result of dissolution. Finally, this review identifies the current knowledge gaps and future research needs for comprehensively identifying the significance of defects in mineral dissolution.

10.
Int J Biol Sci ; 14(14): 2023-2036, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30585266

RESUMO

Atrophic nonunion, a complicated failure of fracture healing, is still obscure regarding its molecular pathological mechanisms. Carboxyl-terminal binding proteins (CtBPs), an NADH-sensitive transcriptional corepressor family, are involved in many diseases, such as cancer and inflammation. Here, we found that CtBP2, but not CtBP1, was significantly overexpressed in atrophic nonunion tissues compared to healthy controls. Using a mass spectrometry assay, we found that CtBP2 can form a complex with histone acetyltransferase p300 and transcription factor Runx2. The lower NADH level in atrophic nonunion tissues disrupted CtBP2 dimerization and enhanced the blockage of the accessibility of the p300-Runx2 complex to the promoters of a series of bone-related target genes, such as OSC, ALPL, COL1A1, IBSP, SPP1 and MMP13. The expression of these genes can be reversed by a forced increase in NADH with CoCl2 treatment. In conclusion, our study revealed that NADH levels determine the expression of bone formation and development of related genes through affecting the dissociation or binding of CtBP2 to the p300-Runx2 complex. These results represent a conserved mechanism, by which CtBP2 serves as a NADH-dependent repressor of the p300-Runx2 transcriptional complex and thus affects bone formation.


Assuntos
Oxirredutases do Álcool/metabolismo , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , NAD/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fatores de Transcrição de p300-CBP/metabolismo , Oxirredutases do Álcool/genética , Células Cultivadas , Imunoprecipitação da Cromatina , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Humanos , Imunoprecipitação , Espectrometria de Massas , Metaloproteinase 13 da Matriz/genética , Metaloproteinase 13 da Matriz/metabolismo , Proteínas do Tecido Nervoso/genética , Ligação Proteica , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Transcrição de p300-CBP/genética
11.
Mol Med Rep ; 18(6): 4895-4903, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30272305

RESUMO

Primary sicca syndrome (pSS) is a systemic autoimmune disease. However, its exact etiology and pathogenesis remain elusive. Various infectious factors have been identified to be closely associated with the occurrence and development of PSS. The present study aimed to assess the composition of the oral microbial flora of patients with pSS in China in order to provide guidance for treatment. The microbial flora of nine patients with pSS and five healthy controls from East China was evaluated in saliva samples using high­throughput sequencing. A high microbial diversity was detected in the pSS and control groups, with bacteroidetes, firmicutes and proteobacteria constituting the largest phyla in the two groups. Compared with the control group, bacteroidetes and actinobacteria were significantly more abundant in the pSS group, whereas proteobacteria were significantly less abundant. However, no significant differences in bacterial richness and diversity were observed between the two groups. According to a Kyoto Encyclopedia of Genes and Genomes linear discriminant analysis, genes regulating cell apoptosis and the immune and digestive systems were significantly upregulated in the pSS group compared with those in the control group. In conclusion, the present study provided basic data on the flora of the oral cavity in patients with pSS from East China and may serve as a reference for the treatment of this condition.


Assuntos
Microbiota , Boca/microbiologia , Síndrome de Sjogren/etiologia , Biodiversidade , Estudos de Casos e Controles , Biologia Computacional/métodos , Feminino , Humanos , Masculino , Metagenoma , Metagenômica/métodos
12.
World J Clin Cases ; 6(10): 322-334, 2018 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-30283795

RESUMO

Labial and oral melanotic macules are commonly encountered in a broad range of conditions ranging from physiologic pigmentation to a sign of an underlying life-threatening disease. Although Laugier-Hunziker syndrome (LHS) shares some features of labial and oral pigmentation with a variety of conditions, it is a benign and acquired condition, frequently associated with longitudinal melanonychia. Herein, the demographic, clinical, dermoscopic, and pathological aspects of LHS were reviewed comprehensively. The important differential diagnoses of mucocutaneous and nail pigmentation are provided. An accurate diagnosis is crucial to design a reasonable medical strategy, including management options, malignant transformation surveillance, and psychological support. It is important that clinicians conduct long-term follow-up and surveillance due to the potential risks of malignant transformation and local severe complications in some conditions.

13.
Environ Sci Pollut Res Int ; 25(31): 30938-30948, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30178416

RESUMO

A novel magnetic silica gel adsorbent (Fe3O4-Si-COOH) was successfully prepared by introducing carboxyl group in situ to improve the performance for Pb(II), Zn(II), and Cd(II) adsorption. Infrared spectroscopy (IR), scanning electron microscope (SEM), transmission electron microscope (TEM), thermo-gravimetric analyzer (TGA), the Brunauer-Emmett-Teller (BET) surface area, X-ray diffraction (XRD), and vibrating sample magnetometer (VSM) characterizations suggested that Fe3O4-Si-COOH has been successfully prepared. The adsorption performance was evaluated by batch experiments with different initial concentrations, ionic strength, contact time, and pH. The adsorption kinetics data followed pseudo-second-order model and exhibited a three-stage intraparticle diffusion mode. Isothermal adsorption equilibrium data were best fitted by the Freundlich model and the adsorption capacity were 155, 110, and 93 mg/g (initial concentration 210 mg/L) for Pb(II), Zn(II), and Cd(II), respectively. The result of X-ray photoelectron spectroscopy (XPS) survey spectrum suggested that the main adsorption mechanism is that the H+ of carboxyl groups exchanged with heavy metal ions in the adsorption processes. In addition, the adsorbed Fe3O4-Si-COOH could be regenerated and the adsorption capacity of reused Fe3O4-Si-COOH could maintain 80.3% after five cycles. Hence, the Fe3O4-Si-COOH could be a kind of potential material for removing Pb(II), Zn(II), and Cd(II) from wastewater. Graphical abstract.


Assuntos
Cádmio/química , Óxido Ferroso-Férrico/química , Chumbo/química , Sílica Gel/química , Poluentes Químicos da Água/química , Zinco/química , Adsorção , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/química , Purificação da Água/métodos
14.
Ultrason Sonochem ; 48: 163-170, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30080538

RESUMO

Though the positive role of ultrasound has been confirmed in the mineral extraction, its potential towards fiercely mechanically-activated mineral was not yet mentioned. In this study, as a novel mechanical activation style, bead milling (BM) was presented and ZnO ore was selected to determine its effectiveness. Results showed that median particle size of ZnO ore could be pulverized to as low as 1/164 of its original value (from ∼29.2 µm to ∼178 nm), indicating much higher activation potential of BM than that of conventional ball milling. Besides, structure destruction, even phase transformation with the direct participation of airborne CO2 occurred. All these processes rendered the superior activation capacity of BM. In view of the extraction promotion, the combination of ultrasound and BM exerted more pronounced effect than those of individual ones, indicating the synergistic effect between extra energy input (by ultrasound) and inner energy storage (by fierce BM). The classic shrinking core model with the product layer diffusion as the rate-controlling step was found to well model the extraction kinetics. The modeling disclosed high capability of ultrasound and BM combination in decreasing the activation energy (Ea) (from 54.6 kJ/mol to 26.4 kJ/mol), while ultrasound, BM could only decrease the Ea to 44.9 kJ/mol, 41.5 kJ/mol, respectively. The dual roles of ultrasound were specially highlighted: (i) participation in the extraction process via direct energy input, (ii) regulation of the aggregation that the activated ore suspension was confronted with.

15.
Sci Rep ; 8(1): 9610, 2018 06 25.
Artigo em Inglês | MEDLINE | ID: mdl-29942097

RESUMO

Osteoarthritis (OA) is a complex degenerative joint disorder, which is caused by both environmental and genetic factors. Previous studies have indicated that the GNL3 gene is associated with knee osteoarthritis (KOA) susceptibility in Europeans; however, the exact molecular mechanism is still unclear. In the present study, we investigated the potential genetic association of GNL3 with KOA in a two-stage sample of 6,704 individuals from the Han Chinese population. Subjects containing 1,052 KOA patients and 2,117 controls were considered the discovery dataset, while subjects consisting of 1,173 KOA patients and 2,362 controls were utilized as the replication dataset. Single-SNP association, imputation, and haplotypic association analyses were performed. The SNP of rs11177 in GNL3 was identified to be significantly associated with KOA after accounting for age, gender and BMI in both stages. The imputed SNP of rs6617 in SPCS1 was found to be strongly associated with KOA risk, and the significant association signal was confirmed in the replication stage. Moreover, a haplotype-based analysis also indicated a positive genetic effect of GNL3 on KOA susceptibility. In summary, our results proved that GNL3 plays an important role in the etiology of KOA, suggesting that GNL3 is a potential genetic modifier for KOA development.


Assuntos
Grupos Étnicos/genética , Proteínas de Ligação ao GTP/genética , Predisposição Genética para Doença/genética , Proteínas Nucleares/genética , Osteoartrite do Joelho/genética , Polimorfismo de Nucleotídeo Único , Idoso , China/etnologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
16.
Oncol Lett ; 15(6): 9889-9895, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29928361

RESUMO

p62 (also known as sequestosome 1) protein, is a small regulatory protein that accumulates in autophagy-defective cells that has been demonstrated to be involved in the prognosis and survival of patients with several types of cancer. However, to the best of our knowledge, there have been no such studies for osteosarcoma (OS). In the present study, the expression of p62 in 70 OS samples was determined using immunohistochemistry and its association with various clinicopathological factors was assessed. The results demonstrated that the overexpression of p62 protein was detected in 77.1% (54/70) samples, and the expression levels were significantly associated with tumor size (P=0.001), metastasis (P=0.036), clinical staging (P=0.003) and poor prognosis (P=0.0058). Furthermore, suppression of the p62 expression by short hairpin RNA interference in F5M2 and F4 cells lines led to decreased cell proliferation, migration and invasion in vitro. These results suggested that increased expression of p62 may be involved in OS progression, and therefore the excess expression of p62 may serve as a novel prognostic biomarker for patients with OS.

17.
Environ Sci Pollut Res Int ; 25(12): 11958-11969, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29450773

RESUMO

The influence of Mn2+ ions on the generation of heavy metal anode slime during zinc electrolysis industry was extensively investigated using several electrochemical methods, electron microscope technologies, and particle size analysis. Results showed that the Mn2+ could obviously promote oxygen evolution reaction (OER) and thereby weaken oxidation efficiency of Mn2+ (ηMnO2) and dissolution of Pb2+. The significant improvement in kinetic parameters for OER was found in electrolytes of 1 and 3 g/L Mn2+, but became unstable as the Mn2+ concentration increased to 10 g/L. This result was correlated with much different properties of oxide layers that its changes of microstructure are involved in, since it confirmed that the positive role of compact oxide layers in contributing to high corrosion resistance and activity for OER, but excessive Mn2+, resulted in its micromorphology of overthickness and instability. Such differences resulted from the effect of the Mn2+ concentration fluctuation on kinetic rates of the nucleation growth process. The formation and adsorption of intermediate MnO2-OHads identified as the controlled step for Mn2+ catalyzing OER was also recommended. The generation mechanism of anode slime was found to be changed in essence due to varying Mn2+ concentrations. In electrolyte of 1 g/L Mn2+, results revealed that the root cause of excessive small suspended anode slime (around 20 µm) was the change of the initial pathway of Mn2+ electro-oxidation, whereas, it showed great improvement in the settling performance as the Mn2+ concentration was increased to 10 g/L. Considering the potential of optimizing Mn2+ concentrations as a cleaner approach to control anode slime, deepening the understanding of the impact mechanism of Mn2+ can provide new insights into intervention in the generation of anode slime.


Assuntos
Eletrólitos/química , Chumbo/química , Óxidos/química , Oxigênio/química , Sulfato de Zinco/química , Zinco/química , Adsorção , Corrosão , Eletrodos , Eletrólise , Metais Pesados , Oxirredução
18.
Mol Med Rep ; 17(3): 4035-4042, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29286133

RESUMO

Osteoarthritis (OA) is a common degenerative joint disease. Inflammation may exaggerate the catabolism and degeneration in the pathogenesis of OA. Hydroxytyrosol (HT) has been used in the management of inflammatory diseases. In addition, reports have revealed that autophagy was a therapeutic target of diseases caused by inflammation. Sirtuin 6 (SIRT6) has also been demonstrated to prevent OA development by reducing both the inflammatory response and chondrocyte senescence. However, the roles of SIRT6 and autophagy in cartilage and its underlying anti­inflammatory mechanism are unknown. Therefore, the present study aimed to determine the effects of HT on autophagy and inflammation in chondrocytes, and clarify whether HT regulates the inflammatory response through SIRT6­mediated autophagy. The expression of protein and mRNA were determined by western blot analysis and reverse transcription­quantitative polymerase chain reaction. The production of cytokines was detected by ELISA. It was demonstrated that HT inhibited the levels of interleukin (IL)­1ß and IL­6 in tumor necrosis factor (TNF)­α­stimulated chondrocytes in a concentration­dependent manner. In addition, HT promoted cell autophagy and increased the mRNA and protein expression levels of SIRT6 in chondrocytes stimulated with TNF-α. Autophagy inhibitor 3-methyladenine or knockdown of SIRT6 decreased the inhibitory effects of HT on the inflammatory response in chondrocytes. In addition, knockdown of SIRT6 attenuated the expression of microtubule-associated protein 1A/1B­light chain 3 and Beclin1 in chondrocytes. Overall, these findings suggested that HT inhibits the inflammatory response of chondrocytes through SIRT6­mediated autophagy. The present study provided a new drug target for the clinical treatment of inflammatory diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Autofagia/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Álcool Feniletílico/análogos & derivados , Sirtuínas/genética , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Adenina/análogos & derivados , Adenina/farmacologia , Animais , Cartilagem Articular/efeitos dos fármacos , Cartilagem Articular/imunologia , Cartilagem Articular/patologia , Sobrevivência Celular/efeitos dos fármacos , Quimiocina CCL2/antagonistas & inibidores , Quimiocina CCL2/genética , Quimiocina CCL2/imunologia , Condrócitos/imunologia , Condrócitos/patologia , Regulação da Expressão Gênica , Interleucina-1beta/antagonistas & inibidores , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Interleucina-6/antagonistas & inibidores , Interleucina-6/genética , Interleucina-6/imunologia , Masculino , Modelos Biológicos , Osteoartrite/genética , Osteoartrite/imunologia , Osteoartrite/patologia , Álcool Feniletílico/farmacologia , Cultura Primária de Células , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Sirtuínas/antagonistas & inibidores , Sirtuínas/imunologia , Fator de Necrose Tumoral alfa/farmacologia
19.
J Dent Sci ; 13(2): 124-130, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30895107

RESUMO

Background/purpose: Recurrent aphthous ulceration (RAU) has an incidence of approximately 20% in general population. However, its exact cause remains unknown. Increasing evidence suggests that immunologic mechanisms may play crucial roles in the etiology of this disease. Materials and methods: The peripheral blood samples were obtained from 85 patients with RAU during acute phase and 87 healthy controls. The serum levels of IgG, IgA, IgM, C3 and C4 were measured by immunoturbidimetry. In addition, the serum IgE levels were measured by electro-chemiluminescence immunoassay. Furthermore, the percentages of B, T, CD4+ T, CD8+ T lymphocytes and natural killer (NK) cells in peripheral blood were determined by flow cytometry. Results: Our findings showed that the serum IgG, IgA, IgE, C3 and C4 levels of RAU patients were significantly higher than those of healthy controls. The percentages of CD4+ T cells and B cells in peripheral blood of RAU patients were significantly decreased, whereas the percentages of CD8+ T cells and NK cells of RAU patients were remarkably increased. Our results indicated that the IgG level was elevated in 18 patients (21.2%) and that the IgE level was increased in 21 patients (24.7%). Our results also showed that the frequency of abnormal IgG or IgE levels were significantly correlated with that of abnormal CD8+ T cell percentage in RAU patients. Conclusion: The levels of both humoral and cellular immune components could be altered in RAU. The relationship between humoral and cellular immune may be potentially important immunologic aspects involved in the pathogenesis of RAU.

20.
J Cancer ; 8(9): 1619-1628, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28775781

RESUMO

Osteosarcoma is the most common bone cancer primarily occurring in children and young adults. Over the past few years, the deregulation of a superfamily transcription factors, known as forkhead box (FOX) proteins, has been demonstrated to contribute to the pathogenesis of osteosarcoma. Molecular mechanism studies have demonstrated that FOX family proteins participate in a variety of signaling pathways and that their expression can be regulated by multiple factors. The dysfunction of FOX genes can alter osteosarcoma cell differentiation, metastasis and progression. In this review, we summarized the evidence that FOX genes play direct or indirect roles in the development and progression of osteosarcoma, and evaluated the emerging role of FOX proteins as targets for therapeutic intervention.

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