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1.
Artigo em Inglês | MEDLINE | ID: mdl-33809199

RESUMO

The coronavirus SARS-CoV-2 responsible for the current human COVID-19 pandemic has shown tropism toward different organs with variable efficiency, eyes included. The purpose of this study has been to investigate the presence of detectable SARS-CoV-2 infection in ocular swabs in patients affected by COVID-19. A consecutive series of 74 COVID-19-positive patients (age 21-89) were enrolled at two Polish COVID-19 hospitals for 4 months and were characterized by PCR for the presence of the SARS-CoV-2 genetic material in nasopharyngeal (NP) and ocular swabs, while their respiratory and ocular symptoms were noted. Almost 50% of them presented with severe/critical respiratory involvement, and some degree of eye disease. No tight correlation was observed between the presence of ocular and respiratory symptoms. Three male patients presenting with severe/critical lung disease tested positive in ocular swab, however with mild/moderate ocular symptoms. In conclusion, our study lends further support to the view that overt ocular infection by the SARS-CoV-2 virus is not such a frequent occurrence.


Assuntos
COVID-19 , Infecções por Coronavirus , Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Polônia , SARS-CoV-2 , Adulto Jovem
2.
J Clin Med ; 10(1)2020 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-33374333

RESUMO

Because the optimal treatment for COVID-19 is still unknown, it is important to explore every potential way of improving the chances of survival for COVID-19 patients. The aim of the study was to analyze the effectiveness of convalescent plasma on COVID-19 patients. The study population consisted of 78 patients diagnosed with COVID-19, selected from the SARSTer national database, who received convalescent plasma. The impact on clinical and laboratory parameters was assessed. A clinical improvement was observed in 62 (79%) patients, and 10 (13%) patients died from COVID-19. No side effects of the convalescent plasma treatment were observed. When plasma was administered earlier than 7 days from diagnosis, the total hospitalization time was shorter (p < 0.05). Plasma efficacy was inferior to remdesivir in endpoints such as the necessity and duration of oxygen therapy, the duration of hospitalization, and mortality rate, and inferior to other drugs in the case of the duration of hospitalization and the necessity of constant oxygen therapy, but comparable in most other measured endpoints. A comparison of a 30-day mortality rate in patients who received plasma and remdesivir (4/25, 16%) and who received only plasma (6/53, 11%) showed no significant difference. Convalescent plasma efficacy is inferior to remdesivir when treating COVID-19 patients but the addition of remdesivir to plasma does not improve the treatment effectiveness. In most endpoints, plasma was comparable to other treatment options. In our opinion, convalescent plasma may be used as a supportive treatment in COVID-19 patients because of the low frequency of adverse effects and availability, but must be given as early from the diagnosis as possible.

3.
Ann Agric Environ Med ; 27(2): 317-318, 2020 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-32588614

RESUMO

INTRODUCTION: Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method. In some cases, such as mutations of the SARS-CoV-2 genome, variable viral load kinetics or laboratory errors, it can be false-negative. CASE REPORT: The case is presented of a 56-year-old man with respiratory tract symptoms, with twice negative results of real-time-reverse transcription-polymerase chain-reaction of nasopharyngeal swabs and positive chest computed tomography, with typical findings for COVID-19 pneumonia. CONCLUSIONS: Patients with negative RT-PCR results, but with positive computed tomography findings characteristic for COVID-19, should be treated as well as those infected.


Assuntos
Betacoronavirus , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/patologia , Nasofaringe/virologia , Pneumonia Viral/diagnóstico , Pneumonia Viral/patologia , COVID-19 , Teste para COVID-19 , Vacinas contra COVID-19 , Técnicas de Laboratório Clínico , Infecções por Coronavirus/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Pandemias , Pneumonia Viral/diagnóstico por imagem , Radiografia Torácica , Reação em Cadeia da Polimerase em Tempo Real , SARS-CoV-2 , Sensibilidade e Especificidade , Tomografia Computadorizada por Raios X
4.
PLoS One ; 15(3): e0230374, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32210468

RESUMO

Oxidative stress is suggested to be the crucial factor in diabetes mellitus type 2 (DM2) pathogenesis and in the development of diabetic complications. Patients with DM2 may be more susceptible to infections due to hyperglycaemia-induced virulence of various microorganisms. Several studies pointed that Epstein-Barr virus (EBV) infection is associated with reactive oxygen species (ROS) production and/or activation of signalling pathways connected with ROS. The present study analyzed serum activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in DM2 patients with and without EBV infection. Blood and saliva were collected from 120 patients with DM2. EBV DNA was detected in the saliva using nested-PCR technique. Spectrophotometric methods were implemented to determine serum GPx and SOD activity with the use of diagnostic kits produced by Randox Laboratories. GPx and SOD activity was decreased in diabetic patients, with the lowest values in DM2 EBV-positive patients. There was correlation between GPx and SOD activity-with increased value of GPx, SOD activity was also rised. In patients with DM2 history longer than 10 years as well as in DM2 patients with obesity, antioxidant enzymes activity was decreased. Determination of examined parameters may be useful in diabetic patients with EBV infection and could be important prognostic factor.


Assuntos
Diabetes Mellitus Tipo 2/sangue , Infecções por Vírus Epstein-Barr/sangue , Glutationa Peroxidase/sangue , Herpesvirus Humano 4/isolamento & purificação , Superóxido Dismutase/sangue , Adulto , DNA Viral/isolamento & purificação , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/imunologia , Infecções por Vírus Epstein-Barr/complicações , Infecções por Vírus Epstein-Barr/imunologia , Infecções por Vírus Epstein-Barr/virologia , Estudos de Viabilidade , Feminino , Glutationa Peroxidase/metabolismo , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/imunologia , Humanos , Masculino , Pessoa de Meia-Idade , Estresse Oxidativo/imunologia , Prognóstico , Espécies Reativas de Oxigênio/imunologia , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/imunologia , Superóxido Dismutase/metabolismo , Adulto Jovem
5.
J Anim Physiol Anim Nutr (Berl) ; 104(1): 352-361, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31538365

RESUMO

The aim of this experiment was to investigate whether the amount of Cu added to the diet of rats can be reduced without adversely affecting the antioxidant status of tissues and growth, and whether copper nanoparticles can be used for this purpose. For four weeks, four experimental groups of rats were fed diets with two dosages of added Cu (standard-6.5 or 3.25 mg/kg) in two forms (standard-CuCO3 or copper nanoparticles). Replacing the CuCO3 supplement with CuNPs resulted in a decreased lung weight and an increased Cu content in brain, kidney and lung, intensification of lipid peroxidation processes, and weakened antioxidant defence in the lungs and kidneys. This treatment also reduced the Cu content in heart, level of lipid oxidation in the liver and testes and improved antioxidant defence in the brain. Reducing the addition of Cu to the diet from 6.5 to 3.25 mg/kg reduced lung weight and increased lipid peroxidation in the liver, heart and lungs, and also weakened antioxidant defence in the lungs and testes. This treatment also weakened the lipid peroxidation process in the spleen, small intestine and brain and strengthened the antioxidant defence of the brain and kidneys. In conclusion, replacing CuCO3 with CuNPs and reducing the level of Cu in the diet of rats has a particularly unfavourable effect on the respiratory system, causing adverse changes in the lungs. However, these treatments have a clearly positive effect on the redox status of the liver and brain.


Assuntos
Cobre/administração & dosagem , Cobre/farmacologia , Dieta/veterinária , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Cobre/química , Relação Dose-Resposta a Droga , Masculino , Nanopartículas Metálicas/química , Oxirredução , Ratos , Ratos Wistar
6.
Postepy Biochem ; 66(4): 385-389, 2020 12 31.
Artigo em Polonês | MEDLINE | ID: mdl-33470071

RESUMO

Viral infections contribute to many cancers worldwide and represent a significant percentage of deaths. Oncogenic viruses include the Epstein-Barr virus, which is the main cause of infectious mononucleosis and exhibits tropism towards B lymphocytes. Due to the presence of genes responsible for latency, it can contribute to many pathological conditions. Examples of this are high-fatal malignancies located in the stomach as well as in the head and neck. Moreover, this virus poses a serious threat to immunocompromised people, which is a significant problem nowadays due to the increasing number of patients undergoing immunosuppressive therapy. Particular attention in this case is lymphoproliferative disorders after transplantation, which are a malignant neoplasm associated with EBV infection. This review focuses on the role of the Epstein-Barr virus in selected cancers.


Assuntos
Infecções por Vírus Epstein-Barr , Transtornos Linfoproliferativos , Neoplasias , Infecções por Vírus Epstein-Barr/complicações , Herpesvirus Humano 4 , Humanos
7.
Anticancer Res ; 37(9): 4853-4858, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28870905

RESUMO

BACKGROUND/AIM: The association between Epstein-Barr virus (EBV) and gastric cancer (GC) has been reported by many researchers. Immunosuppressive cytokines, such as interleukin-10 (IL-10) and transforming growth factor ß (TGFß), play an important role in the tumor process. The aim of the present study was to detect antibodies against EBV and explore the levels of TGFß and IL-10 in Polish GC patients. PATIENTS AND METHODS: Fifty patients with GC and 50 hospitalized individuals without GC (control group) were enrolled in the study. Frozen tumor tissue fragments were tested using nested PCR assay for EBV DNA detection. ELISA test was used to detect the presence of anti-VCA IgG, anti-EBNA IgG, anti-EA IgG, TGFß and Il-10 in sera from all individuals. RESULTS: EBVCA was detected in 88.0%, EBNA in 90.0%, and EA in 72.0% of patients. Levels of TGFß and IL-10 were significantly higher in patients with high levels of anti-EA antibodies (25.4 ng/ml; 7.8 pg/ml) compared to patients with low levels of anti-EA antibodies (12.61 ng/ml; 4.29 pg/ml). CONCLUSION: The significantly higher level of EA in patients' sera indicates EBV reactivation. TGFß level was significantly higher in GC than in the control group, especially in EA-positive patients, indicating its possible role in gastric carcinogenesis.


Assuntos
Biomarcadores Tumorais/sangue , Herpesvirus Humano 4/fisiologia , Interleucina-10/sangue , Neoplasias Gástricas/sangue , Neoplasias Gástricas/virologia , Fator de Crescimento Transformador beta/sangue , Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Estudos de Casos e Controles , Demografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
8.
Postepy Hig Med Dosw (Online) ; 67: 950-7, 2013 Sep 10.
Artigo em Polonês | MEDLINE | ID: mdl-24088538

RESUMO

The heart is a mesoderm-derived organ, whose formation is regulated by various genes. Initially, the most important is expression of Nkx2.5, CR1, pitx2, anf and mhc2a, which are responsible for differentiation of cardiomyocytes. In a later phase activation of mhc2b, pitx2c, mesp1, pcmf1, vmhc, xin, mcl2v, mlc2a, mlc2a, mef2, hand1 and hand2 was revealed. Their expression is regulated by various molecules, including transcription (XIN, GATA, MEF, Tbx5, Baf60c, PECAM, tie-2, MEF2) and growth (VEGF, FGF, PDGF) factors, as well as proteins (i.e., dickkopf-1, cerberus, cytotactin, fibrillin, nodal, thrombomodulin, Wnt, bone morphometric ones - BMP2, BMP 4, BMP5, BMP7) and other substances, such as retinoid and folic acid. Crucial steps in cardiac organogenesis are development of the ventricle and atrial formation, as well as septation and valve formation. Any disturbances of such processes may lead to various congenital heart diseases and defects that could be initiated by various genetic, epigenetic or environmental factors. The most common heart malformations are: stenosis (coarctation) of the aorta and pulmonary trunk, bicuspid aortic valve, atrial and/or ventricular septal defect, persistent truncus arteriosus (Botallo duct), transposition of the great vessels, tricuspid atresia, hypoplastic left and right heart, as well as syndrome of Lutembachera, Cantrell, Ebstein, Eisenmenger and Shone and trilogy, tetralogy, pentalogy of Fallot.


Assuntos
Átrios do Coração/crescimento & desenvolvimento , Cardiopatias/genética , Cardiopatias/patologia , Ventrículos do Coração/crescimento & desenvolvimento , Diferenciação Celular/genética , Proteínas da Matriz Extracelular/metabolismo , Regulação da Expressão Gênica/fisiologia , Cardiopatias Congênitas/genética , Cardiopatias Congênitas/metabolismo , Cardiopatias Congênitas/patologia , Cardiopatias/metabolismo , Valvas Cardíacas/crescimento & desenvolvimento , Humanos , Células Musculares/citologia
9.
Pol Merkur Lekarski ; 32(192): 410-3, 2012 Jun.
Artigo em Polonês | MEDLINE | ID: mdl-22891569

RESUMO

Over-the-counter antipyretics (acetaminophen, aspirin, ibuprofen) and/or analgesics (acetaminophen, aspirin, diclofenac, ibuprofen, naproxen) are relatively safe for adults. However, data on their developmental toxicity is sparse. Moreover, experimental and clinical findings are commonly incompatible. The highest developmental toxicity was found for aspirin. However, unlike full-dose of aspirin, its low doses are compatible during pregnancy. Over-the-counter nonselective cyclooxygenase inhibitors (non-steroidal anti-inflammatory drugs, NSAID) may induce miscarriage, stillbirth, intrauterine growth retardation, preterm constriction of the ductus arteriosus with a secondary persistent pulmonary hypertension, reduced fetal renal perfusion that led to oligohydramion, prolonged pregnancy as well as an increase prevalence of intracranial bleeding in newborns. For acetaminophen and ibuprofen an increased risk of cryptorchidism was also pointed. Prenatal exposure to acetaminophen may also induce bronchial asthma during childhood, preeclampsia, preterm birth, maternal phlebothrombosis and pulmonary embolism. Furthermore, epidemiological data suggest higher risk of cardiac, abdominal wall and orofacial defects of evaluated drugs.


Assuntos
Analgésicos/efeitos adversos , Antipiréticos/efeitos adversos , Doenças Fetais/induzido quimicamente , Doenças do Recém-Nascido/induzido quimicamente , Medicamentos sem Prescrição/efeitos adversos , Complicações na Gravidez/induzido quimicamente , Efeitos Tardios da Exposição Pré-Natal/induzido quimicamente , Adulto , Aspirina/efeitos adversos , Feminino , Humanos , Recém-Nascido , Gravidez
10.
Pol Merkur Lekarski ; 32(187): 34-7, 2012 Jan.
Artigo em Polonês | MEDLINE | ID: mdl-22400177

RESUMO

Marketing in health service has become an indispensable tool for creating and maintaining a positive image of medical institutions which to a great extent determines their success on the market. This process entails not only providing professional, reliable and up-to-date patient care but also establishing good reputation among those who already use it and its potential customers. It should be recognizable also for deliverers, investors, competitors, media and particularly for society and local authorities. The key to success is professionalism of personnel and their identification with the mission of the institution and the direction of changes being implemented there. For a complete success and recognition is essential health care facilities, which affects virtually matched the name and symbol, and communication of people responsible for contact with the media.


Assuntos
Atenção à Saúde/organização & administração , Administração de Instituições de Saúde/métodos , Marketing de Serviços de Saúde/métodos , Polônia , Simbolismo
11.
Pol Merkur Lekarski ; 32(187): 38-40, 2012 Jan.
Artigo em Polonês | MEDLINE | ID: mdl-22400178

RESUMO

Irrespective of changing standards and continuous reforms in health service, the patient should always remain the principal focus. The patient is a person who should be treated not only as a customer or a recipient of medical services but also as a person being in a situation which is difficult and often unacceptable. Among the factors affecting patients' satisfaction and contentment in the course of the disease it is profoundly important that he cooperates with his doctor. For years it has been attempted to create an ideal model of doctor-patient relationship which would be mutually beneficial and not violating privacy or welfare of any of them. These attempts focused on various theoretical models, among others paternalistic, informative and interpretive. However, special attention should be paid to adherence to principles which is based on respect for patient autonomy, harmlessness, charity and fairness.


Assuntos
Marketing de Serviços de Saúde/organização & administração , Modelos Organizacionais , Satisfação do Paciente , Relações Médico-Paciente , Humanos , Paternalismo , Participação do Paciente , Polônia
12.
Pol Merkur Lekarski ; 32(187): 46-9, 2012 Jan.
Artigo em Polonês | MEDLINE | ID: mdl-22400180

RESUMO

Permanent changes occurring in economy and politics, open borders, a greater number of people with higher education as well as growing social awareness concerning taking care of health are followed by changes in healthcare sector. In response to an increased social demand, new medical institutions are being founded. They offer their patients a vast array of services ranging from therapeutic to preventive and highly specialized procedures improving their standard of living. In order to attract regular patients, and therefore receive regular income, it is necessary to stabilize such institutions on services market. It can be achieved by numerous marketing tools which, when applied appropriately, enable to create a positive image of a medical institution.


Assuntos
Marketing de Serviços de Saúde/métodos , Marketing de Serviços de Saúde/organização & administração , Polônia
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