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1.
J Acoust Soc Am ; 152(4): 2204, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-36319246

RESUMO

This paper presents a practical acoustic source that can implement the third-order super-cardioid beam pattern (SCBP). Based on the product theorem of the array, the third-order dipole beam pattern can be implemented based on the first-order and second-order dipole beam patterns so that a four-element array is designed. In addition, the second-order dipole beam pattern can also be implemented by the same four-element array. Hence, a four-element array is designed to implement the third-order SCBP. Subsequently, the single-sided radiation flextensional transducer-which is a type of low-frequency underwater acoustic transducer-is selected as the array element of the four-element array that is studied and analyzed by means of the finite element method. Finally, a four-element array prototype is fabricated and tested leading to the conclusion that the special four-element array combined with the product theorem provides a practical way for the implementation of the third-order SCBP.

2.
Nanoscale ; 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36367049

RESUMO

Room temperature sodium-sulfur (RT Na-S) batteries show extraordinary potential in large-scale energy storage. MXenes have been demonstrated to be promising sulfur hosts for Na-S batteries, and their surface functional groups play a pivotal role in their performance. However, the effect of different surface functional groups of MXenes on their anchoring effect and catalytic performance has not been systematically investigated. Herein, density functional theory (DFT) calculations were employed to explore the various electrochemical performances of a series of Ti2CTx (T = O, S, N, F, Cl, and Br) MXenes as sulfur hosts for Na-S batteries. We find that surface functional groups significantly affect the structural properties of MXenes as well as their electrochemical performance. Ti2CO2, Ti2CS2, and Ti2CN2 exhibit prominent affinity toward soluble sodium polysulfides. Moreover, they display excellent catalytic activity toward the sulfur reduction reaction and the decomposition reaction of Na2S. Finally, during the whole discharge process, Ti2CO2, Ti2CS2, and Ti2CN2 always maintain their metallic conductivity, which could improve the rate capability of Na-S batteries. Overall, Ti2CO2, Ti2CS2, and Ti2CN2 are proposed as promising bifunctional sulfur hosts for Na-S batteries, and our results may also provide insights for modulating the performance of MXenes in other applications.

3.
Molecules ; 27(21)2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36364326

RESUMO

N-nitroso-N-methyl-4-aminobutyric acid (NMBA) is the third N-nitrosamine impurity found in sartans. Herein, a sensitive and stable LC-MS/MS method with multiple reactions monitoring mode has been developed for the quantitative determination of NMBA in four sartan substances. The effective separation of NMBA and sartan substances was achieved on a C18 column under gradient elution conditions. The mass spectrometry method of the atmospheric pressure chemical ionization source and internal standard method was selected as the quantitative analysis method of NMBA. Then, this proposed LC-MS/MS analysis method was validated in terms of specificity, sensitivity, linearity, accuracy, precision and stability. Good linearity with correlation coefficient over 0.99 was obtained at the NMBA concentration of 3-45 ng/mL, and the limit of quantification was 3 ng/mL. Additionally, the recoveries of NMBA in four sartan substances ranged from 89.9% to 115.7%. The intra-day and inter-day relative standard deviation values were less than 5.0%. In conclusion, this developed determination method for NMBA through liquid chromatography-tandem mass spectrometry showed the characteristics of good sensitivity, high accuracy and precision, which will be of great help for the quantitative analysis of NMBA in sartan products.


Assuntos
Bloqueadores do Receptor Tipo 1 de Angiotensina II , Espectrometria de Massas em Tandem , Cromatografia Líquida/métodos , Espectrometria de Massas em Tandem/métodos , Cromatografia Líquida de Alta Pressão/métodos , Dano ao DNA , Ácido gama-Aminobutírico , Reprodutibilidade dos Testes
4.
Chemosphere ; 312(Pt 1): 137171, 2022 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-36370755

RESUMO

Bisphenol A (BPA), an important environmental pollutant, is known to damage reproductive development. However, the underlying epigenetic mechanism in Leydig cells during BPA exposure has not been explored in detail. In this study, TM3 Leydig cells were treated with BPA (0, 20, 40 and 80 µM) for 72 h. The differentially expressed TET1 cell model was constructed to explore the mechanism of BPA-induced cytotoxicity. Results showed that BPA exposure significantly inhibited cell viability and increased apoptosis of TM3 Leydig cells. Meanwhile, the mRNA of TET1, Cav3.2 and Cav3.3 decreased significantly with the increase of BPA exposure. Importantly, TET1 significantly promoted proliferation of TM3 Leydig cells and inhibited apoptosis. Differentially expressed TET1 significantly affected BPA-induced toxicity in TM3 Leydig cells. Notably, TET1 elevated the mRNA levels of Cav3.2 and Cav3.3. MeDIP and hMeDIP confirmed that TET1 regulated the expression of Cav3.3 through DNA hydroxymethylation. Our study firstly presented that TET1 participated in BPA-induced toxicity in TM3 Leydig cells through regulating Cav3.3 hydroxymethylation modification. These findings suggest that TET1 acts as a potential epigenetic marker for reproductive toxicity induced by BPA exposure and may provide a new direction for the research on male reproductive damage.

5.
Pathol Res Pract ; 240: 154177, 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36327815

RESUMO

OBJECTIVES: To explore the Fibroblast-epithelial metabolic coupling among laryngeal cancers and its prognostic roles METHODS: We reviewed the clinical information of patients with laryngeal cancer in our department. Paraffin-embedded tissues from included patients were immune-stained with antibodies towards MCT4 and TOMM20 and evaluated for stromal and epithelial expression. Survival analysis and Cox regression analysis were applied to investigate the prognostic factor of laryngeal squamous cell carcinoma. TCGA database was used to validate our result. RESULTS: Stromal MCT4 and TOMM20 were both significantly associated with each other among laryngeal cancer tissues. High expression of both Stromal MCT4 and TOMM20 is related to poor prognosis in laryngeal cancer. Stromal MCT4 expression was an independent prognostic indicator for laryngeal cancer. Furthermore, cancer cell MCT4 expression has no relationship with the clinical characteristics of laryngeal cancer. CONCLUSIONS: Our results support that the phenomenon of metabolic symbiosis was exist in the laryngeal cancer tissue. In addition, TOMM20 and stromal MCT4 could be used as new therapeutic targets for laryngeal cancer.

6.
Inorg Chem ; 61(43): 16981-16985, 2022 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-36251482

RESUMO

Metal-organic framework (MOF) glass is a new type of glass material, but it usually lacks sufficient porosity. Thus, regulating the pore structure of MOF glass to improve its adsorption performance is very important. Herein, we found that the porosity of MOF glasses agZIF-62 and agZIF-76 can be regulated via an ammonia-immersion approach. After ammonia immersion, the resulting agZIF-62-NH3 and agZIF-76-NH3 could be maintained in their glass states or converted to their amorphous states, respectively. Their porosity changed according to the gas adsorption experiments. Notably, compared with agZIF-62 and agZIF-76, the iodine uptake capacities for agZIF-62-NH3 and agZIF-76NH3 increased by 12 and 21 times, respectively. This work shows that the subsequent treatment of MOF glass can regulate their adsorption performance.

7.
Front Microbiol ; 13: 993285, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36312959

RESUMO

Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes. However, it is unclear whether gut microbiota is distinct between CRCs with different MMR status. In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients. The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing. The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored. Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level. In our study cohort, dMMR status was identified in 29 of 230 (12.61%) tumors. The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues. The microbial community composition (beta-diversity) between the two groups was significantly different. The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level. However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level. Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status. KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group. These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.

8.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 36(10): 1296-1304, 2022 Oct 15.
Artigo em Chinês | MEDLINE | ID: mdl-36310469

RESUMO

Objective: To develop an anti-inflammatory poly (lactic-co-glycolic acid) (PLGA) scaffold by loading xanthohumol, and investigate its anti-inflammatory and cartilage regeneration effects in goats. Methods: The PLGA porous scaffolds were prepared by pore-causing agent leaching method, and then placed in xanthohumol solution for 24 hours to prepare xanthohumol-PLGA scaffolds (hereinafter referred to as drug-loaded scaffolds). The PLGA scaffolds and drug-loaded scaffolds were taken for general observation, the pore diameter of the scaffolds was measured by scanning electron microscope, the porosity was calculated by the drainage method, and the loading of xanthohumol on the scaffolds was verified by Fourier transform infrared (FTIR) spectrometer. Then the two scaffolds were co-cultured with RAW264.7 macrophages induced by lipopolysaccharide for 24 hours, and the expressions of inflammatory factors [interleukin 1ß (IL-1ß) and tumor necrosis factor α (TNF-α)] were detected by RT-PCR and Western blot to evaluate the anti-inflammatory properties in vitro of two scaffolds. Bone marrow mesenchymal stem cells (BMSCs) was obtained from bone marrow of a 6-month-old female healthy goat, cultured by adherent method, and passaged in vitro. The second passage cells were seeded on two scaffolds to construct BMSCs-scaffolds, and the cytocompatibility of scaffolds was observed by live/dead cell staining and cell counting kit 8 (CCK-8) assay. The BMSCs-scaffolds were cultured in vitro for 6 weeks, aiming to verify its feasibility of generating cartilage in vitro by gross observation, histological staining, collagen type Ⅱ immunohistochemical staining, and biochemical analysis. Finally, the two kinds of BMSCs-scaffolds cultured in vitro for 6 weeks were implanted into the goat subcutaneously, respectively. After 4 weeks, gross observation, histological staining, collagen type Ⅱ immunohistochemical staining, biochemical analysis, and RT-PCR were performed to comprehensively evaluate the anti-inflammatory effect in vivo and promotion of cartilage regeneration of the drug-loaded scaffolds. Results: The prepared drug-loaded scaffold had a white porous structure with abundant, continuous, and uniform pore structures. Compared with the PLGA scaffold, there was no significant difference in pore size and porosity ( P>0.05). FTIR spectrometer analysis showed that xanthohumol was successfully loaded to PLGA scaffolds. The in vitro results demonstrated that the gene and protein expressions of inflammatory cytokines (IL-1ß and TNF-α) in drug-loaded scaffold significantly decreased than those in PLGA scaffold ( P<0.05). With the prolongation of culture, the number of live cells increased significantly, and there was no significant difference between the two scaffolds ( P>0.05). The in vitro cartilage regeneration test indicated that the BMSCs-drug-loaded scaffolds displayed smooth and translucent appearance with yellow color after 6 weeks in vitro culture, and could basically maintained its original shape. The histological and immunohistochemical stainings revealed that the scaffolds displayed typical lacunar structure and cartilage-specific extracellular matrix. In addition, quantitative data revealed that the contents of glycosaminoglycan (GAG) and collagen type Ⅱ were not significantly different from BMSCs-PLGA scaffolds ( P>0.05). The evaluation of cartilage regeneration in vivo showed that the BMSCs-drug-loaded scaffolds basically maintained their pre-implantation shape and size at 4 weeks after implantation in goat, while the BMSCs-PLGA scaffolds were severely deformed. The BMSCs-drug-loaded scaffolds had typical cartilage lacuna structure and cartilage specific extracellular matrix, and no obvious inflammatory cells infiltration; while the BMSCs-PLGA scaffolds had a messy fibrous structure, showing obvious inflammatory response. The contents of cartilage-specific GAG and collagen type Ⅱ in BMSCs-drug-loaded scaffolds were significantly higher than those in BMSCs-PLGA scaffolds ( P<0.05); the relative gene expressions of IL-1ß and TNF-α were significantly lower than those in BMSCs-PLGA scaffolds ( P<0.05). Conclusion: The drug-loaded scaffolds have suitable pore size, porosity, cytocompatibility, and good anti-inflammatory properties, and can promote cartilage regeneration after implantation with BMSCs in goats.


Assuntos
Engenharia Tecidual , Tecidos Suporte , Animais , Feminino , Tecidos Suporte/química , Engenharia Tecidual/métodos , Colágeno Tipo II , Cabras , Fator de Necrose Tumoral alfa , Cartilagem , Anti-Inflamatórios/farmacologia , Células Cultivadas
9.
Ear Nose Throat J ; : 1455613221135649, 2022 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-36257626

RESUMO

Sinonasal teratocarcinosarcoma (SNTCS) is one of the rarest and most highly invasive malignant neoplasms often found in the nasal cavity and paranasal sinuses. SNTCS is often misdiagnosed because of its morphological heterogeneity. Due to its rarity, clinical characteristics and optimal therapy have not been well-established. Here, we present a case of SNTCS with orbital and intracranial extensions. A 48-year-old male patient presented with left-side nasal obstruction for 3 years. He appeared with visual and neurological symptoms 2 months ago. On radiographic examination, a mass was observed in the left paranasal sinuses with orbital and intracranial extension involvement. The mass was surgically resected. In the future, knowledge of this entity may assist in the accurate diagnosis and proper management of SNTCS.

10.
Front Neurosci ; 16: 947295, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36188456

RESUMO

Schizophrenia (SCZ) is a serious mental illness that affects 1% of people worldwide. SCZ is associated with a higher risk of developing metabolic disorders such as obesity. Antipsychotics are the main treatment for SCZ, but their side effects include significant weight gain/obesity. Despite extensive research, the underlying mechanisms by which SCZ and antipsychotic treatment induce weight gain/obesity remain unclear. Hypothalamic endoplasmic reticulum (ER) stress is one of the most important pathways that modulates inflammation, neuronal function, and energy balance. This review aimed to investigate the role of hypothalamic ER stress in SCZ and antipsychotic-induced weight gain/obesity. Preliminary evidence indicates that SCZ is associated with reduced dopamine D2 receptor (DRD2) signaling, which significantly regulates the ER stress pathway, suggesting the importance of ER stress in SCZ and its related metabolic disorders. Antipsychotics such as olanzapine activate ER stress in hypothalamic neurons. These effects may induce decreased proopiomelanocortin (POMC) processing, increased neuropeptide Y (NPY) and agouti-related protein (AgRP) expression, autophagy, and leptin and insulin resistance, resulting in hyperphagia, decreased energy expenditure, and central inflammation, thereby causing weight gain. By activating ER stress, antipsychotics such as olanzapine activate hypothalamic astrocytes and Toll-like receptor 4 signaling, thereby causing inflammation and weight gain/obesity. Moreover, evidence suggests that antipsychotic-induced ER stress may be related to their antagonistic effects on neurotransmitter receptors such as DRD2 and the histamine H1 receptor. Taken together, ER stress inhibitors could be a potential effective intervention against SCZ and antipsychotic-induced weight gain and inflammation.

11.
Nurs Open ; 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36302641

RESUMO

AIM: The aim of the study was to evaluate the psychometric properties of the Chinese version of the Self-Directed Learning Readiness Scale. DESIGN: A cross-sectional survey was designed. METHODS: A convenience sample of 659 participants was recruited. Exploratory and confirmatory factor analyses were used to evaluate the factorial structure. In addition, the concurrent validity was assessed using the Critical Thinking Disposition Inventory. RESULTS: The exploratory factor analysis revealed that the scale was a 6-factor structure, named love of learning, active learning, effective learning, independent learning, learning motivation and creative learning, which explained 53.30% of the total variance. The findings of the confirmatory factor analysis showed that a 37-item six first-order model produced the best-fit statistics. Internal consistency for the scale was satisfactory, ranging from 0.71 to 0.88. Concurrent and predictive validities also reached significant levels. The utility of the instrument was suggested.

12.
Psychol Res Behav Manag ; 15: 2859-2877, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36217378

RESUMO

Purpose: The COVID-19 pandemic has greatly influenced the health and lifestyles of individuals. Increasing numbers of consumers now participate in quantified self (QS) process to learn more about their health-related behaviors. Understanding how to increase consumers' QS continuance participation intention is critical. Drawing on Social Cognitive Theory and Self-Construal Theory, this study investigates how the presentation characteristics of QS data and consumers' self-construal can influence their continuance participation intention during QS process. Methods: Three between-subjects scenario simulation experiments were conducted to examine the influence mechanisms of the presentation mode and type of QS data and self-construal on consumers' continuance participation intention. Results: The study found: (1) the presentation mode (horizontal comparison vs vertical comparison) and type (descriptive vs analytic) of QS data had significant interaction effects on consumers' continuance participation intention; (2) consumers' self-construal (interdependent vs independent) and the presentation mode of QS data had obvious interaction effects on their continuance participation intention; and (3) consumers' self-construal and the presentation type of QS data had interaction influences on their continuance participation intention. Conclusion: This research combined Social Cognitive Theory and Self-Construal Theory to analyze the influence mechanisms of the presentation characteristics of QS data and consumers' self-construal on their continuance participation intention. These findings not only expand the research field and the scope of application of Social Cognitive Theory, but also provide new insights for the study of consumers' QS problems. They have reference value for the optimization of the presentation features of QS data, and for improving the match between QS data presentation and consumers' self-construal types, to motivate continued participation in QS process.

13.
Sci Rep ; 12(1): 16885, 2022 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-36207478

RESUMO

Somatostatin, a growth hormone-release inhibiting peptide, exerts antiproliferative and antiangiogenic effects on tumor cells. However, the short half-life of somatostatin limits its application in human therapy, and long-acting somatostatin fusion protein is also limited by its severe terminal degradation. Therefore, oncolytic virus delivery system was introduced to express somatostatin fusion protein and the anti-tumor effects of both somatostatin and oncolytic virus were combined to destroy tumor tissues. Here, a vaccinia VG9/(SST-14)2-HSA recombinant was constructed by replacing somatostatin fusion gene into TK locus of attenuated VG9 strain via homologous recombination. Results showed that vaccinia VG9/(SST-14)2-HSA possessed a combined anti-tumor effect on sstr-positive tumor cells in vitro. In the tumor burden models, BALB/c mice with complete immunity are most suitable for evaluating tumor regression and immune activation. Complete tumor regression was observed in 3 out of 10 mice treated with vaccinia VG9/TK- or VG9/(SST-14)2-HSA, and the survival of all mice in both groups was significantly prolonged. Besides, vaccinia VG9/(SST-14)2-HSA is more effective in prolonging survival than VG9/TK-. Vaccinia VG9/(SST-14)2-HSA exerts a combined anti-tumor efficacy including the oncolytic ability provided by the virus and the anti-tumor effect contributed by (SST-14)2-HSA, which is expected to become a potent therapeutic agent for cancer treatment.


Assuntos
Neoplasias , Terapia Viral Oncolítica , Vaccinia , Animais , Hormônio do Crescimento/metabolismo , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias/metabolismo , Terapia Viral Oncolítica/métodos , Receptores de Somatostatina/genética , Receptores de Somatostatina/metabolismo , Somatostatina/genética , Somatostatina/metabolismo , Vírus Vaccinia
14.
Nat Commun ; 13(1): 5990, 2022 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-36220818

RESUMO

The universe abounds with solid helium in polymorphic forms. Therefore, exploring the allotropes of helium remains vital to our understanding of nature. However, it is challenging to produce, observe and utilize solid helium on the earth because high-pressure techniques are required to solidify helium. Here we report the discovery of room-temperature two-dimensional solid helium through the diamond lattice confinement effect. Controllable ion implantation enables the self-assembly of monolayer helium atoms between {100} diamond lattice planes. Using state-of-the-art integrated differential phase contrast microscopy, we decipher the buckled tetragonal arrangement of solid helium monolayers with an anisotropic nature compressed by the robust diamond lattice. These distinctive helium monolayers, in turn, produce substantial compressive strains to the surrounded diamond lattice, resulting in a large-scale bandgap narrowing up to ~2.2 electron volts. This approach opens up new avenues for steerable manipulation of solid helium for achieving intrinsic strain doping with profound applications.

15.
J Acoust Soc Am ; 152(3): 1709, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36182290

RESUMO

A continuously steerable super-cardioid beam pattern (SCBP) has advantages such as controllable direction, narrower beam width, and higher gain. It is generally implemented with a uniform circular array (UCA). Here, an implementation method of continuously steerable SCBPs is proposed for a projector array. The synthesis and the implementation of the continuously steerable SCBP are studied and analyzed based on a UCA model composed of omnidirectional sources. An improved version of the common method is proposed. It requires fewer array elements and the massive power amplifiers matched to each array element, which reduces the cost and the installation space. Subsequently, a continuously steerable SCBP is implemented with a uniform circular projector array (UCPA). This system is composed of single-sided radiation flextensional transducers and is studied and analyzed with a finite element method, which verified that the proposed method is suitable for the underwater acoustic projector array. Finally, UCPA prototypes were fabricated. The tests demonstrated that the proposed implementation method is a flexible way for the application of the continuously steerable SCBP in the low-frequency emission field.

16.
Oxid Med Cell Longev ; 2022: 2158644, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36193072

RESUMO

As one of the most common cancer chemotherapy drugs, cisplatin is widely used in cancer management. However, cisplatin-induced nephrotoxicity occurs in patients who receive this drug. This study is aimed at developing therapeutic agents that effectively alleviate the nephrotoxic effects during cisplatin treatment. We identified a compound named pyrocatechol (PCL) from a natural product library that significantly alleviated cisplatin-induced cytotoxicity in vitro. Pyrocatechol treatment substantially ameliorated cisplatin (20 mg · kg-1) treatment-induced neuropathological indexes, including inflammatory cell infiltration and apoptosis, in vivo. Mechanistically, pyrocatechol significantly prevented oxidative stress-induced apoptosis by activating glutathione peroxidase 4 (GPX4) to reduce reactive oxygen species (ROS) accumulation in cisplatin-treated cells. In addition, pyrocatechol significantly inhibited ROS-induced JNK/P38 activation. Thus, we found that pyrocatechol prevents ROS-mediated JNK/P38 MAPK activation, apoptosis, and cytotoxicity through GPX4. Our study demonstrated that pyrocatechol is a novel therapeutic agent against cisplatin-induced kidney injury.


Assuntos
Injúria Renal Aguda , Antineoplásicos , Produtos Biológicos , Injúria Renal Aguda/patologia , Antineoplásicos/farmacologia , Apoptose , Produtos Biológicos/uso terapêutico , Catecóis/farmacologia , Catecóis/uso terapêutico , Cisplatino/farmacologia , Humanos , Rim/patologia , Estresse Oxidativo , Fosfolipídeo Hidroperóxido Glutationa Peroxidase , Espécies Reativas de Oxigênio/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
17.
Acta Biomater ; 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-36206975

RESUMO

Adipose-derived mesenchymal stem cells (ADSCs) are multipotent stromal cells and play huge role in forming and repairing bone tissues. Emerging evidence shows that MicroRNAs (miRNAs) are involved in ADSCs differentiation. Here, we explored the role of miR-150-5p and its related mechanisms in ADSCs osteogenesis. Real-time PCR was used to determine miR-150-5p expression during ADSCs osteogenesis. miR-150-5p inhibitors, miR-150-5p ADV or short hairpin RNA (shRNA) of Notch3 were transfected to ADSCs for analyzing the effects on osteogenesis.  The mixture of hydroxyapatite/tricalcium phosphate (HA/TCP) ceramic powders and transfected ADSCs was implanted into BALB/C nude mice. Micro-CT and histological methods were performed to evaluate the new bone formation. Compared with negative control (NC) and miR-150-5p overexpression, inhibition of miR-150-5p increased ADSCs osteogenesis by regulating Notch3. MiR-150-5p overexpression decreased the expression of pFAK, pERK1/2, and RhoA, while these were up-regulated when miR-150-5p was inhibited, or notch3 was silenced. Furthermore, miR-150-5p inhibition partially reversed the suppression effect of notch3 knockdown on osteogenesis in vitro and in vivo. This study demonstrated the critical function of miR-150 during osteogenesis. The combination of ADSCs with miR-150-5p inhibition and HA/TCP might be a promising strategy for bone damage repair. STATEMENT OF SIGNIFICANCE: : Osteoporosis is a common chronic metabolic bone disease in humans. Bone tissue engineering based on mesenchymal stem cells, biomaterials, and growth factors, provides a promising way to treat osteoporosis and bone defects. ADSCs commonly differentiate into adipose cells, they can also differentiate into osteogenic cell lineages. Nucleic acids and protein have usually been considered as regulators of ADSCs osteogenic differentiation. In the current study, we demonstrated the combination of ADSCs with miR-150-5p inhibition and hydroxyapatite/tricalcium phosphate ceramic powders enhanced bone regeneration. Furthermore, miR-150-5p/Notch3 axis regulating osteogenesis via the FAK/ERK1/2 and RhoA pathway was assessed. The current study showed the application of ADSCs in bone regeneration might be a promising strategy for osteoporosis and bone damage repairing.

18.
Adv Mater ; : e2207682, 2022 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-36208070

RESUMO

Zn-based solid polymer electrolytes (SPEs) have enormous potential in realizing high-performance zinc-ion batteries. Polymeric single-ion conductor (PSIC)-based SPEs can largely eradicate anion migration and side reactions of electrodes with decreased polarization, but the ionic conductivity is still unsatisfactory due to the tight localized ion interactions and sluggish chain motion. Herein, by employing the heterocyclic tetrazole as the anionic center of the side chain, a novel PSIC is fabricated with optimized charge delocalization and enhanced side-chain motion. The as-prepared PSIC delivers an ionic conductivity up to 5.4 × 10-4 S cm-1 with an ultrahigh Zn2+ transference number of 0.94. Based on the PSIC, dendrite-free and hydrogen-free Zn plating/stripping cycling (2000 h) is achieved. A further assembled Zn‖V2 O5 battery exhibits superior performances to other solid ZIBs, including a high discharge capacity, excellent rate capability, and long cycling life. In addition, a remarkable shelf-life (90 d), low self-discharge rate, and good temperature adaptability of the solid battery can be achieved benefiting from the high stability of the SPE during operation. The PSIC-based SPEs with advanced ion-transport structure endow solid ZIBs with significant performance improvement, high safety, and durability.

19.
Angew Chem Int Ed Engl ; : e202214461, 2022 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-36289047

RESUMO

Hydrogen isotope exchange (HIE) represents one of the most attractive labeling methods to synthesize deuterium- and tritium-labeled compounds. Catalytic HIE methods that enable site-selective C-H bond activation and exchange labeling with gaseous isotopes D2 and T2 are of vital importance, in particular for high-specific-activity tritiation of pharmaceuticals. As part of our interest in exploring s-block metals for catalytic transformations, we found CsN(SiMe3 )2 to be an efficient catalyst for selective HIE of benzylic C-H bonds with D2 gas. The reaction proceeds through a kinetic deprotonative equilibrium that establishes an exchange pathway between C-H bonds and D2 gas. By virtue of multiple C-H bonds activation and high activity (isotope enrichment up to 99 %), the simple cesium amide catalyst provided a very powerful and practically convenient labeling protocol for synthesis of highly deuterated compounds and high-specific-activity tritiation of pharmaceuticals.

20.
Photodiagnosis Photodyn Ther ; 40: 103132, 2022 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-36167260

RESUMO

There is a lack of a simple and reliable method for the diagnosis of postoperative bile leakage. ICG is a water-soluble fluorescent contrast agent. After intravenous injection of ICG, ICG is eventually excreted only through the biliary tract system into the intestines. It is highly sensitive, stable and nonradioactive, and these characteristics provide a theoretical basis for using ICG to diagnose postoperative bile leakage. Two patients with postoperative bile leakage were successfully identified using indocyanine green fluorescent imaging for the first time, suggesting its potential to be a convenient and reliable method for the diagnosis of postoperative bile leakage.

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