Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Biol Macromol ; 51(1-2): 153-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22542852

RESUMO

Cordyceps militaris SU5-08 was derived from an initial strain (C. militaris SU5) by ultraviolet mutagenesis of protoplasts, and the extraction parameters for C. militaris SU5-08 exopolysaccharide (EPS) produced during submerged culture were optimized. The extraction rate of EPS was 1919.16±165.27 mg/l, which was 120.38±11.36% higher than that of C. militaris SU5. The in vitro scavenging effects of EPS of C. militaris SU5-08 on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals at a dosage of 5 g/l were 63.64±3.52%, 75.27±5.16%, and 6.46±5.03%, respectively. The reducing power of EPS of C. militaris SU5-08 was 0.21±0.01. The results suggest that the EPS of C. militaris SU5-08 can be used as a potential antioxidant which enhances adaptive immune responses.


Assuntos
Antioxidantes/farmacologia , Cordyceps/metabolismo , Polissacarídeos/farmacologia , Cordyceps/genética , Radical Hidroxila/metabolismo , Mutação , Oxirredução/efeitos dos fármacos , Polissacarídeos/isolamento & purificação , Superóxidos/metabolismo
2.
J Biosci Bioeng ; 111(1): 50-4, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20801714

RESUMO

Response surface methodology (RSM) was used to optimize the extraction parameters for Pholiota adiposa SX-02 intracellular polysaccharide (IPS) produced during submerged culture. The optimum conditions of IPS extraction were predicted to be, ultrasonic power at 564.93 W, precipitation time 30.34 h and pH 8.28, and IPS yield was estimated at 19.75%. The actual value of IPS under these conditions was 20.51%. The in vitro antioxidant results showed that the inhibition effects of IPS at a dosage of 250 mg/l on superoxide anion, hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 74.66 ± 5.31%, 69.20 ± 5.13%, and 75.20 ± 6.73%, respectively, which were 27.51 ± 2.23%, 16.58 ± 1.33%, and 9.46 ± 0.72% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of IPS was 0.32 ± 0.02 (absorbance at 700 nm), 39.13 ± 3.47% higher than that of BHT. The results provide a reference for large-scale extraction of IPS by P. adiposa SX-02 in industrial fermentation and the IPS can be used as a potential antioxidant which enhances adaptive immune responses.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Pholiota/metabolismo , Polissacarídeos/isolamento & purificação , Compostos de Bifenilo/metabolismo , Meios de Cultura , Depuradores de Radicais Livres/farmacologia , Concentração de Íons de Hidrogênio , Radical Hidroxila/metabolismo , Picratos/metabolismo , Polissacarídeos/farmacologia
3.
Int J Biol Macromol ; 47(2): 116-9, 2010 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-20580645

RESUMO

The extraction conditions of intracellular polysaccharide (IPS) from Pleurotus sp. mycelium in submerged culture were investigated. Four parameters affecting the IPS extraction, ultrasonic treatment time, extraction temperature, extraction time and ethanol concentration, were determined by single factor tests and then optimized by orthogonal experiments. Under the optimized conditions, the extraction rates of IPS of Pleurotus nebrodensis SJ-02, Pleurotus eryngii SI-01 and Pleurotus corncopiae SS-01 were 7.1+/-0.4%, 7.5+/-0.3%, and 8.2+/-0.5%, respectively. The in vitro hydroxyl radical inhibition percentages of IPS of three mushrooms were 32.2+/-2.8%, 16.1+/-1.5%, and 38.7+/-3.1%, respectively. The scavenging effects of IPS on superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were 19.1+/-1.5%, 16.3+/-1.3%, 20.3+/-1.8%, 17.9+/-1.6%, 16.8+/-1.4%, and 20.5+/-1.7%, respectively. The results provide a reference for large-scale production of IPS by Pleurotus sp. in industrial fermentation.


Assuntos
Depuradores de Radicais Livres/isolamento & purificação , Depuradores de Radicais Livres/farmacologia , Espaço Intracelular/química , Micélio/citologia , Pleurotus/citologia , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Compostos de Bifenilo/metabolismo , Depuradores de Radicais Livres/metabolismo , Radical Hidroxila/metabolismo , Picratos/metabolismo , Polissacarídeos/metabolismo , Superóxidos/metabolismo
4.
Bioresour Technol ; 101(12): 4564-9, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20153962

RESUMO

Response surface methodology was used to optimize extraction parameters for Morchella esculenta SO-01 exopolysaccharide (EPS) produced during submerged culture. The optimum conditions for EPS extraction were predicted to be, concentration at 84.07 degrees C, precipitation for 22.19 h and pH 8.44, and EPS production was estimated at 5.45 g/L. The actual yield of EPS under these conditions was 5.32 g/L. The antioxidant capacity of the EPS was measured in vivo after filling the stomach with different doses of EPS and results showed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of blood, spleen, liver, heart, and kidney were increased by 125%, 46.11%, 23.33%, 12.19%, 41.29%, and 63.24%, 63.12%, 166.54%, 98.01%, 57.68%, respectively, and that malonaldehyde (MDA) of blood, spleen, liver, heart, and kidney were decreased by 21.80%, 67.84%, 28.48%, 56.15%, 41.62%. The results provide a reference for large-scale extraction of EPS by M. esculenta SO-01 in industrial fermentation.


Assuntos
Agaricales/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Biotecnologia/métodos , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Análise de Variância , Animais , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Camundongos , Especificidade de Órgãos/efeitos dos fármacos , Superóxido Dismutase/metabolismo
5.
Biol Trace Elem Res ; 131(1): 81-9, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19252827

RESUMO

Selenium (Se) is an essential trace element for humans and animals. Stropharia rugoso-annulata is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this study were to investigate the Se-enrichment characteristics of S. rugoso-annulata in submerged culture and evaluate the antioxidant activities of Se-enriched mycelia in vivo in terms of the values of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA). The optimum parameters of Se-enrichment under the optimal Se concentration (150 microg/mL) in media were as follows: biomass 8.11 +/- 0.25 g/L, Se content in mycelia 4,727.68 +/- 13 microg/g, Se-accumulated rate 24.68 +/- 1.67%, and percentage of organic Se 96.27 +/- 3.26%. The mainly subsistent forms of selenium in Se-enriched mycelia were selenoprotein and selenium-polysaccharide. The contents of total amino acids (TAA) and essential amino acids (EAA) in Se-enriched mycelia were increased by 13.5 +/- 1.09% and 12.8 +/- 0.89%, respectively. It was efficient for Se-enriched mycelia to elevate GSH-Px and SOD activities and decrease MDA content. These results indicated that Se-enriched mycelia of S. rugoso-annulata represent a novel dietary source of bioavailable supplemental selenium.


Assuntos
Agaricales/efeitos dos fármacos , Antioxidantes/farmacologia , Micélio/efeitos dos fármacos , Selênio/farmacologia , Agaricales/metabolismo , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Micélio/metabolismo , Superóxido Dismutase/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA