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1.
Cell Cycle ; 18(19): 2454-2464, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31411527

RESUMO

In a variety of cancers, long non-coding RNAs (lncRNAs) were believed to play important roles. Nevertheless, H19's possible molecular mechanism related to miR-20b-5p has not yet been explored in endometrial cancer. Differential lncRNAs in endometrial cancer were identified based on microarray analysis (GSE23339). In this research, in the first place, H19 expression was detected to be increased but miR-20b-5p to be decreased in endometrial cancer tissues and cells. Besides, H19 expression displayed a negative relationship to miR-20b-5p expression in endometrial cancer tissues. According to gain- and loss-of-function experiments of H19, like a ceRNA, H19 elevated AXL level and HIF-1α expression so as to stimulate the migration, proliferation and EMT process of endometrial cancer. Additionally, the knockdown of H19 slowed down tumor growth, promoted apoptosis and upregulated miR-20b-5p expression but lowered the expressions of HIF-1α, PCNA and AXL in vivo. Furthermore, H19 was also verified to stimulate the activity of endometrial cancer with AXL inhibitor BGB324 in vitro and in vivo. To sum up, H19 accelerates the tumor formation of endometrial cancer through the miR-20b-5p/AXL/HIF-1α signaling pathway, thereby providing a novel target for diagnosing and treating endometrial cancer.

2.
Exp Ther Med ; 14(1): 127-130, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28672902

RESUMO

The aim of the present study was to investigate the effect of rutin treatment on the expression of glycogen synthase kinase (GSK)-3ß and tumor necrosis factor (TNF)-α in A549 human lung carcinoma cells. The A549 cells were divided into control, cisplatin and rutin (low, middle and high) groups. ELISA and western blot analysis of TNF-α expression, 4',6-diamino-2-phenylindole (DAPI) staining and GSK-3ß immunofluorescence staining were used to investigate the effect of rutin in the human lung carcinoma cells, using cisplatin as a positive control. TNF-α expression was significantly higher in the rutin and cisplatin groups compared with the control group. Additionally, DAPI staining revealed that the number of apoptotic cells was higher in the rutin and cisplatin groups compared with the control group, and immunofluorescence showed that the expression of GSK-3ß in the cisplatin and rutin groups was significantly higher compared with that in the control group. The results of the present study suggest that rutin promotes the TNF-α-induced apoptosis of A549 human lung carcinoma cells. Furthermore, rutin may be able to regulate the expression of GSK-3ß protein in these cells.

3.
Lipids Health Dis ; 14: 72, 2015 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-26174150

RESUMO

BACKGROUND: In this study, using a meta-analysis approach, we examined the correlation between serum levels of lysophosphastidic acid (LPA) and ovarian cancer (OC). METHODS: Relevant published studies were identified from multiple scientific literature databases by using a pre-determined electronic and manual search strategy. The search results were screened through a multi-step process to select high-quality case-control studies suitable for the present meta-analysis. Mean values and standardized mean differences (SMD) were calculated for plasma LPA levels. Two investigators independently extracted the data from the studies and performed data analysis using STATA software version 12.0 (Stata Corp, College Station, TX, USA). RESULTS: Nineteen case-control studies met our selection criteria and contained a total of 980 OC patients, 872 benign controls and 668 healthy controls. Our meta-analysis results revealed that the plasma levels of LPA in OC patients were significantly higher than benign controls (SMD = 2.36, 95% CI: 1.61-3.10, P < 0.001) and healthy controls (SMD = 2.32, 95% CI: 1.77-2.87, P < 0.001). Subgroup analysis by ethnicity showed that the plasma LPA levels in OC patients were significantly higher than the benign controls only in Asian populations (SMD = 2.52, 95% CI: 1.79-3.25, P < 0.001). However, a comparison between healthy controls and OC patients revealed that, in both Asians and Caucasians, the OC patients displayed significantly higher plasma LPA levels compared to healthy controls (all P < 0.05). CONCLUSION: Our meta-analysis showed strong evidence that a significantly higher plasma LPA levels are present in OC patients, compared to benign controls and healthy controls, and plasma LPA levels may be used as a biomarker or target of OC.


Assuntos
Lisofosfolipídeos/sangue , Neoplasias Ovarianas/sangue , Adulto , Idoso , Estudos de Casos e Controles , Feminino , Humanos , Pessoa de Meia-Idade , Viés de Publicação
4.
Int J Clin Exp Pathol ; 8(2): 1427-34, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25973027

RESUMO

The expression of CD133 decreases with differentiation of tumor cell, indicating that CD133 is a specific marker for isolation and identification of CSCs. In the present study the effect of Ursolic acid chalcone (UAC) on CD133(+) hepatocellular carcinoma cell (HCC CSCs) differentiation, their self-renewal, tumorigenic capacity and sensitivity to chemotherapeutic drugs was studied. The results demonstrated that UAC inhibits the expression of CD133(+) in a dose and time-dependent manner in PLC/PRF/5 and Huh7 HCC cells. The inhibition was significant at 50 µM and on day 8. The percentage of CD133(+) cells decreased from an initial 59.3% in PLC/PRF/5 to 37.1% and 78.2% in Huh7 to 59.2% on treatment with UAC. There was inhibition of Oct4, Tert, Bmi1, ß-catenin, ABCG2, and tumor sphere-related gene Ep300. In addition it also decreased number of CK19-positive cells and increased number of CK8/18-positive cells. UAC treatment caused a decrease in self-renewal capability and increase in sensitivity to doxorubicin and vincristine drugs in CD133(+) HCC CSCs. Therefore, UAC can be a potent therapeutic agent to target differentiation of CSC in HCC.


Assuntos
Antineoplásicos/farmacologia , Carcinoma Hepatocelular/patologia , Chalcona/farmacologia , Neoplasias Hepáticas/patologia , Células-Tronco Neoplásicas/efeitos dos fármacos , Triterpenos/farmacologia , Antígeno AC133 , Antígenos CD/metabolismo , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Separação Celular , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Glicoproteínas/metabolismo , Humanos , Peptídeos/metabolismo
5.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 43(1): 51-7, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24616461

RESUMO

OBJECTIVE: To investigate the expression of neural salient serine/arginine-rich protein 1 (NSSR1) in the development of mouse brain. METHODS: Brain samples were collected from mice with different developmental stages: 9, 12, 14 d before birth (E9, E12, E14) and 1 d, 3 weeks and 3 months after birth. The expression of NSSR1 in mouse brain at different developmental stages was detected by Western blot and the distribution of NSSR1 was analyzed by immunohistochemical staining. The expression and distribution of NSSR1 in mouse brain were compared among embryos, neonatal and adult animals. RESULTS: During embryogenesis, the expression of NSSR1 proteins increases significantly from 0.186(E9) to 0.445(E14) and reached a high level after birth. Immunohistochemical analysis showed that in E12 embryos, NSSR1 was specifically distributed in the marginal and mantle layers. The expression of NSSR1 in hippocampus was very low in neonatal animals but stronger in adults. In cerebellar cortex, NSSR1 was widely expressed in purkinje and granule cells of adult animals, but mainly expressed in Purkinje cells in neonates. CONCLUSION: The expression of NSSR1 is regulated by the development of mouse brain and presents dynamic changes.


Assuntos
Encéfalo/embriologia , Encéfalo/crescimento & desenvolvimento , Proteínas de Ciclo Celular/metabolismo , Proteínas de Neoplasias/metabolismo , Proteínas de Ligação a RNA/metabolismo , Proteínas Repressoras/metabolismo , Animais , Encéfalo/metabolismo , Camundongos
6.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(9): 2425-8, 2013 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-24369645

RESUMO

In the present paper, DMSO/H2O mixture with the ratio of volume 1:1 was measured in the cooling process by Raman spectroscopy, and the Raman assignments was made to the DMSO molecular and water molecular. The results showed that the behavior between intra-molecular hydrogen bonds and inter-molecular hydrogen bonds of DMSO and water leads to the change in the Raman spectra of the S==O stretching vibration of DMSO and the O--H stretching vibration of water. Further analysis showed that the hydrogen bond between DMSO and water was enhanced in the course of temperature decreasing process (27 to -30 degrees C), and the intramolecular hydrogen bonds between water and water replaced the intermolecular hydrogen bonds of DMSO and water in the course of temperature decreasing process (-30 to -60 degrees C). The research provides experimental basis for hydrogen bonding theory in aqueous solution.

7.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(9): 2311-4, 2013 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-24371847

RESUMO

A visible absorption and Raman spectra of all-trans-beta-beta-carotene was measured in cyclohexanol solution in the temperature range from 68 degrees C to 26 degrees C. The results indicated that the visible absorption spectra are red-shifted, Raman scattering cross section increases, Huang-Ryes factor and electron-phonon coupling constants of CC bond vibration modes decreases with the temperature decreasing. The changes are interpreted using the theory of "coherent weakly damped electronic-lattice vibration model" and "effective conjugation length model". The red shift of the absorption spectra and intensity of the Raman active are attributed to the thermal conformational change-induced increase in the effective conjugation length in all-trans-beta-carotene chains. All-trans-beta-carotene has strong coherent weakly damped CC bonds vibrational properties, which lead to large Raman scattering cross section in the solvent of low temperature. The electron-phonon coupling constants with dimension are used, which can easily establish relation with the Huang-Rhys factor and calculate the electron-phonon coupling constants of CC bond vibration modes. Effective conjugation length, the pi-electron delocalization range and the Raman scattering cross section are described by the electron-phonon coupling constants.


Assuntos
Análise Espectral Raman , Temperatura , beta Caroteno/análise , Elétrons , Solventes , Vibração
8.
Zhonghua Yi Xue Za Zhi ; 93(17): 1341-4, 2013 May 07.
Artigo em Chinês | MEDLINE | ID: mdl-24029486

RESUMO

OBJECTIVE: To explore the effects of GRP78 suppression on the sensitivity to cisplatin and elucidate the role and mechanism of GRP78 in ovarian cancer cisplatin resistance. METHODS: The GRP78 siRNA expression plasmid was constructed to suppress GRP78 expression. Cell viability was detected by methyl thiazolyl tetrazolium (MTT) assay. Endoplasmic reticulum stress-related apoptosis related protein expressions were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. And cell apoptosis was detected by flow cytometry. RESULTS: The expressions of GRP78, CHOP and cleaved-caspase 4 were induced significantly by cisplatin (6 mg/L) in SKOV3 cells. And the expression of GRP78 was induced significantly by cisplatin in SKOV3/DDP cells. But the expressions of CHOP and cleaved-caspase 4 showed no significant difference. Inhibition of GRP78 expression and cisplatin combined treatment significantly increased the expressions of cleaved-caspase 4 and cleaved-caspase 3 in SKOV3/DDP cells. Inhibition of GRP78 expression reduced the cisplatin-induced up-regulations of p-Akt and p-mTOR and induced XBP1 mRNA shear expression and CHOP mRNA expression. CONCLUSION: Inhibition of GRP78 expression reverses cisplatin resistance in SKOV3/DDP cells. The mechanism may be through the activity of Akt/mTOR signaling pathway, CHOP expression levels and caspase activity.


Assuntos
Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos , Proteínas de Choque Térmico/metabolismo , Neoplasias Ovarianas/metabolismo , Caspase 3/metabolismo , Caspases Iniciadoras/metabolismo , Linhagem Celular Tumoral , Proteínas de Ligação a DNA/metabolismo , Feminino , Humanos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Fatores de Transcrição de Fator Regulador X , Transdução de Sinais , Serina-Treonina Quinases TOR/metabolismo , Fator de Transcrição CHOP/metabolismo , Fatores de Transcrição/metabolismo , Proteína 1 de Ligação a X-Box
9.
Zhonghua Yi Xue Za Zhi ; 93(9): 659-62, 2013 Mar 05.
Artigo em Chinês | MEDLINE | ID: mdl-23751742

RESUMO

OBJECTIVE: To explore the expression and role of glucose-regulated protein 78 in ovarian serous adenocarcinoma. METHODS: The surgical specimens were collected from 126 cases of serous ovarian tumors and 30 cases of normal ovarian tissue. And the mRNA and protein expressions of GRP78 were detected by immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. RESULTS: The results of immunohistochemistry showed that GRP78 positive cells in ovarian serous adenocarcinoma were significantly higher than those in normal ovarian and ovarian serous cystadenoma tissues (P < 0.05). In ovarian serous adenocarcinoma, GRP78 expression was not associated with pathological grade, age and clinical staging (P > 0.05). And the mRNA expressions of GRP78 in normal ovarian tissue, ovarian serous cystadenoma and ovarian serous cystadenocarcinoma were 0.134 ± 0.021, 0.121 ± 0.032 and 0.685 ± 0.085 respectively. Protein expressions of GRP78 in normal ovarian tissue, ovarian serous cystadenoma and ovarian serous cystadenocarcinoma were 0.211 ± 0.042, 0.193 ± 0.032 and 0.770 ± 0.074 respectively. GRP78 mRNA and protein expression had no difference between different pathological grades of ovarian serous cystadenocarcinoma (P > 0.05). CONCLUSION: GRP78 is highly expressed in ovarian serous adenocarcinoma. And its differential expression between ovarian benign and malignant tumors is significant and correlated with the occurrence and progression of ovarian cancer.


Assuntos
Cistadenocarcinoma Seroso/metabolismo , Proteínas de Choque Térmico/metabolismo , Neoplasias Ovarianas/metabolismo , Adulto , Idoso , Estudos de Casos e Controles , Cistadenocarcinoma Seroso/patologia , Feminino , Humanos , Imuno-Histoquímica , Pessoa de Meia-Idade , Neoplasias Ovarianas/patologia , RNA Mensageiro/genética
10.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 30(2): 189-94, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23568733

RESUMO

OBJECTIVE: To investigate the epidemiological status of glucose-6-phosphate dehydrogenase (G6PD) deficiency among children from Yunnan with unique ethnic origins. METHODS: DNA samples from 11759 children were tested with fluorescent spot test, G6PD/6PGD quantitative ratio assay and hemoglobin electrophoresis. RESULTS: The detection rate of G6PD deficiency was 2.5%, for which boys were significantly greater than girls (3.5% vs. 1.4%, P<0.05). Significant differences were also detected among children from different ethnic groups and different regions. For ethnic Han Chinese, the detection rate was 0.7%, which was lower than the majority of ethnic minorities. By regression analysis, altitude of residence and family history both have significant influence on the calculated rate. CONCLUSION: Occurrence of G6PD deficiency seems to be influenced by gender. It also varies substantially between different ethnic groups as well as regions, e.g., more common in south. It also showed a declining trend after years of diagnosis and intervention. This survey may provide a valuable basis for counseling of G6PD deficiency in Yunnan.


Assuntos
Deficiência de Glucosefosfato Desidrogenase/etnologia , Criança , Pré-Escolar , China/etnologia , Feminino , Deficiência de Glucosefosfato Desidrogenase/genética , Humanos , Lactente , Recém-Nascido , Modelos Logísticos , Masculino
11.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 28(5): 579-82, 2011 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-21983740

RESUMO

OBJECTIVE: To investigate the carrier rate of thalasaemia among the children of 10 minority ethnic groups in 3 border states (Xishuangbanna, Dehong and Nujiang) of Yunnan Province. METHODS: A total of 6562 samples of children under 10 years old were analyzed by blood cell automatic analysis and hemoglobin electrophoresis. RESULTS: The overall carrier frequency of thalasaemia was highest (46.2%) in Dehong, and lowest (30.6%) in Nujiang. The carrier frequency of beta-thalasaemia was the highest (40.6%) in Achang, and lowest (2.5%) in Dulong. The carrier frequency of alpha-thalasaemia was the highest (22.1%) in Dai from Xishuangbanna, followed by Dulong (19.1%). CONCLUSION: Thalasaemia carrier rates were high among the children of 10 minority ethnic groups in Yunnan. There were regional differences in their incidences. The results provide a valuable basis for thalasaemia prevention in Yunnan minorities in the three border states.


Assuntos
Grupos Minoritários , Talassemia/etnologia , Criança , Pré-Escolar , China/epidemiologia , Hemoglobinas Anormais/genética , Humanos , Lactente , Recém-Nascido , Prevalência , Talassemia/genética , Talassemia alfa/epidemiologia , Talassemia alfa/genética , Talassemia beta/epidemiologia , Talassemia beta/genética
12.
Zhonghua Liu Xing Bing Xue Za Zhi ; 32(4): 352-6, 2011 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-21569665

RESUMO

OBJECTIVE: To investigate the prevalence rate of thalassemia among children of 0 - 7 years old, from six ethnic groups in Xishuangbanna and Dehong, Yunnan province. METHODS: 4973 blood samples from children under 7 years old were automatically undergone blood cell count, red cell osmotic fragility and hemoglobin electrophoresis testings. RESULTS: The incidence rates of thalassaemia, ß-thalassemia was 37.4%, and α-thalassaemia were 22.6% and 14.7% respectively. The thalassaemia incidence rates were significantly different among age groups but not in gender. The incidence of α-thalassaemia was decreasing along with the increase of age, while the incidence of ß-thalassaemia was increasing along with the increase of age. Xishuangbanna had the higher incidence than in Dehong and the differences were significant between counties, The incidence of thalassemia of Mengla ranked the first (52.2%) in Xishuangbanna, The differences between different regions and different nationalities were significant, with ß-thalassemia of Achang ranked the first (40.6%), The incidence of α-thalassemia among Han ranked the first as 45.5% while α-thalassaemia and ß-thalassemia were different in regions. α-thalassaemia and ß-thalassemia were significantly different between different ethnic people in the same regions. Multiple factor analysis showed that region seemed to be a risk factor and the mother's ethnicity was a protective factor and dependent variable on thalassaemia. CONCLUSION: The incidence of thalassaemia in Yunnan Xishuangbanna and Dehong was high among children under the age of 7 and were related to ethnic and regional differences in the areas. Specific genes were proliferated along with the extension of time. Our data provided valuable information on prevention and genetic studies on thalassaemia in the minorities of Xishuangbanna and Dehong in Yunnan province.


Assuntos
Talassemia/etnologia , Criança , Pré-Escolar , China/epidemiologia , Grupos Étnicos , Feminino , Humanos , Lactente , Masculino
13.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 35(6): 585-9, 2006 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-17177327

RESUMO

OBJECTIVE: To demonstrate the possibility of high-level expression of bioactive human beta-defensin-2 (hBD2) in E.coli, and to purify the recombinant hBD2. METHODS: DNA fragment containing mature hBD2 coding region (smhBD2-cDNA) was amplified by PCR, multiple copies of smhBD2-cDNA were linked using Bgl II and BamH I enzymes, pET32-nsmHBD2-cDNA with 1, 2, 4, or 8 copies of smhBD2-cDNA was constructed. The soluble and insoluble hBD2 proteins were separated and analyzed by SDS-PAGE analysis. The soluble protein underwent a separation process containing affinity chromatography, enterokinase digestion and ion exchange chromatography to get the recombinant hBD2 peptide. The bioactivity of recombinant hBD2 was examined by bacteria-inhibition tests in liquid culture. RESULT: The plasmids pET32-nsmHBD2-cDNA with 1, 2, 4 copies of smhBD2-cDNA were constructed and the expressed soluble protein accounted for 52 %, 48 %, and 31 % respectively. The plasmids with 8 copies expressed mainly insoluble protein with few in soluble form. The growth of E.coli K12D31 was dramatically suppressed with a inhibition rate of 90 %, when the final concentration of recombinant hBD2 reached between 0.4 to 0.5 mug/ml. CONCLUSION: Fusion expression of human beta-defensin-2 with multiple joined genes in E.coli could increase the expression of hBD2.


Assuntos
Escherichia coli/genética , Proteínas Recombinantes/isolamento & purificação , beta-Defensinas/biossíntese , Anti-Infecciosos/farmacologia , Cromatografia de Afinidade , Eletroforese em Gel de Poliacrilamida , Escherichia coli/efeitos dos fármacos , Expressão Gênica , Humanos , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/farmacologia , beta-Defensinas/genética
14.
Zhonghua Fu Chan Ke Za Zhi ; 40(2): 103-5, 2005 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15840289

RESUMO

OBJECTIVE: To study the association of single nucleotide polymorphism (SNP) at locus +46 of human beta(2)-adrenoceptor (beta(2)-AR) gene with pregnancy induced hypertension (PIH) in women from northern China. METHOD: One hundred and eight women with PIH (PIH group) and ninety seven normal pregnant women (control group) were selected. SNP at locus +46 of human beta(2)-AR was genotyped using PCR-based restriction fragment length polymorphism (RFLP) method. RESULTS: (1) The frequencies of AA, AG and GG genotypes beta(2)-AR at locus +46 were 24.1%, 25.0% and 50.9% in PIH group, respectively. They were 24.7%, 29.9%, and 45.4% in control group, respectively (P > 0.05). The frequencies of the two alleles A and G were 36.6%, 63.4% in PIH group, and 39.7% and 60.3% in control group, respectively (P > 0.05). (2) There was a significant difference in the distribution frequency of beta(2)-AR at locus +46 between mild, moderate and severe degrees of PIH (P < 0.05). CONCLUSION: It indicates that, SNP at locus +46 of beta(2)-AR gene is not associated with generation of PIH in northern China, but it has an association with the severity of PIH.


Assuntos
Hipertensão Induzida pela Gravidez/genética , Polimorfismo de Nucleotídeo Único/genética , Receptores Adrenérgicos beta 2/genética , Adulto , Alelos , Feminino , Frequência do Gene , Genótipo , Humanos , Reação em Cadeia da Polimerase/métodos , Gravidez
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