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1.
Medicine (Baltimore) ; 99(5): e19043, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32000452

RESUMO

BACKGROUND: Propofol has been used widely as an anesthetic for elderly patients; however, the drug instructions only indicate that the need for maintenance of general anesthesia in elderly patients is reduced, and not the extent of the reduction. This study has summarized the usage of propofol in total intravenous anesthesia under bispectral index (BIS) monitoring and determined the optimum dosage of propofol for elderly patients. METHODS: The study comprised 156 patients undergoing elective surgery under general anesthesia divided into 2 groups according to their age: the elderly group (O group) and nonelderly group (Y group). BIS monitoring was used in both groups during the operation, and propofol and remifentanil were used to maintain anesthesia. The preoperative special conditions, intraoperative maintenance of propofol, remifentanil, fentanyl, cis-atracurium, vasoactive drug use, and hemodynamic changes were summarized. RESULTS: Propofol maintenance in the O group was 3.372 ±â€Š0.774 mg/(kg h), which was significantly lesser than that in Y group (P < 0.05). The incidence of cardiovascular and cerebrovascular diseases and the use rate of vasoactive drugs in the O group were significantly higher than in the Y group (P < 0.05). CONCLUSION: Propofol maintenance in the O group was significantly lower than that in the nonelderly group; this indicates that the anesthetic drug delivery rate for elderly patients should be reduced.


Assuntos
Anestésicos Intravenosos/administração & dosagem , Monitores de Consciência , Propofol/administração & dosagem , Idoso , Humanos
2.
Chemosphere ; 247: 125864, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31931318

RESUMO

In this work, a series of δ-MnO2/USY with different contents of δ-MnO2 (0.3 wt%, 1.5 wt%, 3.0 wt%, 10.0 wt%, and 15.0 wt%) were prepared. In addition, their performances of the adsorption of toluene, degradation and mineralization of toluene, and removal of ozone (O3) were investigated. The results showed that, among all the samples, 3.0 wt% δ-MnO2/USY displayed the best performance of toluene adsorption, degradation and mineralization. Furthermore, according to the in situ DRIFTS and GC-MS analysis, the intermediate by-products during the toluene degradation progress were ascertained and the possible pathway of catalytic oxidation toluene by δ-MnO2/USY in the presence of O3 was proposed.

3.
Nutrients ; 12(1)2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31936037

RESUMO

Hyperlipidemia is associated with metabolic disorders, but the detailed mechanisms and related interventions remain largely unclear. As a functional food in Asian diets, Herba houttuyniae has been reported to have beneficial effects on health. The present research was to investigate the protective effects of Herba houttuyniae aqueous extract (HAE) on hyperlipidemia-induced liver and heart impairments and its potential mechanisms. Male C57BL/6J mice were administered with 200 or 400 mg/kg/day HAE for 9 days, followed by intraperitoneal injection with 0.5 g/kg poloxamer 407 to induce acute hyperlipidemia. HAE treatment significantly attenuated excessive serum lipids and tissue damage markers, prevented hepatic lipid deposition, improved cardiac remodeling, and ameliorated hepatic and cardiac oxidative stress induced by hyperlipidemia. More importantly, NF-E2 related factor (Nrf2)-mediated antioxidant and peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α)-mediated mitochondrial biogenesis pathways as well as mitochondrial complex activities were downregulated in the hyperlipidemic mouse livers and hearts, which may be attributable to the loss of adenosine monophosphate (AMP)-activated protein kinase (AMPK) activity: all of these changes were reversed by HAE supplementation. Our findings link the AMPK/PGC-1α/Nrf2 cascade to hyperlipidemia-induced liver and heart impairments and demonstrate the protective effect of HAE as an AMPK activator in the prevention of hyperlipidemia-related diseases.

4.
Parasitol Res ; 118(12): 3195-3204, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31724068

RESUMO

Whole genomic sequencing (WGS) and comparative genomics are increasingly used in the characterization of Cryptosporidium spp. They are facilitated by the establishment of procedures for WGS analysis of clinical specimens without laboratory propagation of pathogens. Results of recent comparative genomics analysis suggest that gene duplication might be associated with broad host ranges of some zoonotic Cryptosporidium species and subtypes, while genetic recombination could be involved in the emergence of virulent subtypes. The availability of WGS data has further facilitated the development of advanced molecular typing tools. The use of these tools together with comparative genomics analyses has begun to improve the investigations of outbreaks in industrialized nations. More WGS data, however, are needed from both industrialized nations and developing countries before we can have in-depth understanding of the population genetics and evolution of Cryptosporidium spp. and genetic determinants of various phenotypic traits in human-pathogenic subtypes.


Assuntos
Criptosporidiose/epidemiologia , Cryptosporidium/genética , Genômica , Animais , Criptosporidiose/parasitologia , Criptosporidiose/transmissão , Cryptosporidium/classificação , Cryptosporidium/patogenicidade , Genoma de Protozoário/genética , Genótipo , Especificidade de Hospedeiro/genética , Humanos , Epidemiologia Molecular , Virulência/genética
5.
Brain Imaging Behav ; 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31628591

RESUMO

Crohn's disease (CD), a chronic inflammatory bowel disease, involved in brain structural and functional changes, including the amygdala. Amygdala is a key structure in the limbic system and its related circuits are implicated in processing of emotion, pain and sensory. However, limited study of the amygdala is elucidated in CD. This study mainly investigated altered functional connectivity (FC) of the amygdala in CD patients during resting-state. Magnetic resonance imaging scans were acquired from 42 CD patients and 35 healthy controls (HCs). Whole amygdala bilaterally were selected as regions of interest (ROIs). Voxel-based morphometry and FC methods were applied to investigate the differences of structure or intrinsic connectivity of the amygdala between the two groups, separately. Pearson correlations were performed to explore relationships between the clinical characteristics and neuroimaging findings in CD patients. Based on the whole amygdala bilaterally as ROIs, compared with HCs, CD patients showed no statistical differences of grey matter destiny but exhibited decreased FC between the amygdala and insula, parahippocampus, as well as anterior middle cingulate cortex/dorsal anterior cingulate cortex. CD patients had negative correlation between the disease duration and amygdala-insula connectivity. In the patient group, patients with higher anxiety or depression scores revealed increased FC of the amygdala with thalamus and orbitofrontal cortex. Our results reveal that aberrant FC of the amygdala may be involved in processing of visceral pain and sensation, and emotion in CD. These findings may further enhance the understanding of neural mechanisms of CD.

6.
Genes (Basel) ; 10(10)2019 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-31581635

RESUMO

Root system plays an essential role in water and nutrient acquisition in plants. Understanding the genetic basis of root development will be beneficial for breeding new cultivars with efficient root system to enhance resource use efficiency in maize. Here, the natural variation of 13 root and 3 shoot traits was evaluated in 297 maize inbred lines and genome-wide association mapping was conducted to identify SNPs associated with target traits. All measured traits exhibited 2.02- to 21.36-fold variations. A total of 34 quantitative trait loci (QTLs) were detected for 13 traits, and each individual QTL explained 5.7% to 15.9% of the phenotypic variance. Three pleiotropic QTLs involving five root traits were identified; SNP_2_104416607 was associated with lateral root length (LRL), root surface area (RA), root length between 0 and 0.5mm in diameter (RL005), and total root length (TRL); SNP_2_184016997 was associated with RV and RA, and SNP_4_168917747 was associated with LRL, RA and TRL. The expression levels of candidate genes in root QTLs were evaluated by RNA-seq among three long-root lines and three short-root lines. A total of five genes that showed differential expression between the long- and short-root lines were identified as promising candidate genes for the target traits. These QTLs and the potential candidate genes are important source data to understand root development and genetic improvement of root traits in maize.

7.
ACS Omega ; 4(6): 11135-11140, 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-31460212

RESUMO

In this work, the study of photocatalytic hydrogen production by RGO/ZnIn2S4 with simultaneous degradation of organic amines was carried out in the presence of organic amines in wastewater as the sacrificial agents. The effects of several factors, such as organic amine types, pH value, catalyst concentration, organic amine concentration, and sunlight source, on the photocatalytic activity of RGO/ZnIn2S4 for H2 production were investigated. At the same time, its performance of degrading organic amines during H2 production was also examined. The results showed that the order of H2 production activity of RGO/ZnIn2S4 in six organic amine solutions was N(CH2CH3)3 > N(HOCH2CH2)3 > N(CH3)3 > HO(CH2)2NH2 > C6H5-N2> CO(NH2)2, and the highest H2 production was in N(CH2CH3)3 (triethylamine) solution, being 1597 µmol·g-1·h-1, which is 2.6 times as high as that using the aqueous solution mixture of Na2S and Na2SO3 as the sacrificial agent. In addition, when the pH was 13, the catalyst concentration was 1.0 g·L-1, and the triethylamine concentration was 1.0 mol·L-1, the photocatalytic activity was the highest. Furthermore, the relationship between triethylamine concentration and H2 production was analyzed according to the theory of dynamics.

8.
J Cell Mol Med ; 23(9): 5934-5948, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31298493

RESUMO

Prostate cancer (PCa) is the second leading cause of cancer-related death in males, primarily due to its metastatic potential. The present study aims to identify the expression of microRNA-539 (miR-539) in PCa and further investigate its functional relevance in PCa progression both in vitro and in vivo. Initially, microarray analysis was conducted to obtain the differentially expressed gene candidates and the regulatory miRNAs, after which the possible interaction between the two was determined. Next, ectopic expression and knock-down of the levels of miR-539 were performed in PCa cells to identify the functional role of miR-539 in PCa pathogenesis, followed by the measurement of E-cadherin, vimentin, Smad4, c-Myc, Snail1 and SLUG expression, as well as proliferation, migration and invasion of PCa cells. Finally, tumour growth was evaluated in nude mice through in vivo experiments. The results found that miR-539 was down-regulated and DLX1 was up-regulated in PCa tissues and cells. miR-539 was also found to target and negatively regulate DLX1 expression, which resulted in the inhibition of the TGF-ß/Smad4 signalling pathway. Moreover, the up-regulation of miR-539 or DLX1 gene silencing led to the inhibition of PCa cell proliferation, migration, invasion, EMT and tumour growth, accompanied by increased E-cadherin expression and decreased expression of vimentin, Smad4, c-Myc, Snail1 and SLUG. In conclusion, the overexpression of miR-539-mediated DLX1 inhibition could potentially impede EMT, proliferation, migration and invasion of PCa cells through the blockade of the TGF-ß/Smad4 signalling pathway, highlighting a potential miR-539/DLX1/TGF-ß/Smad4 regulatory axis in the treatment of PCa.

9.
Life Sci ; 231: 116558, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31194993

RESUMO

AIMS: We aimed to explore the role of SIRT6 in Insulin resistance (IR). We are the first to investigate on this crucial relationship in an obese mouse model fed on a high-fat diet (HFD) and an IR model based on the mature 3T3-L1-derived adipocytes. MAIN METHODS: Western blotting (WB) and qPCR analysis were performed to evaluate the SIRT6 protein and mRNA expressions in HFD mice as well as IR cells. Injection of adenovirus encoding SIRT6 gene in HFD mice and transfection of pcDNA3-SIRT6 in IR cells increased the glucose uptake levels and insulin sensitivity. KEY FINDINGS: The positive regulatory effects of SIRT6 on transient receptor potential vallinoid 1 (TRPV1) in IR cells were confirmed by a mechanistic investigation at both protein and mRNA levels. Further, the overexpression of SIRT6 was found to activate the TRPV1/Calcitonin gene-related peptide (CGRP) signaling and upregulate the glucose transporter (GLUT) expression at protein and mRNA levels. Additionally, administration of the TRPV1 antagonist, SB-705498 repressed the insulin sensitivity upregulated by SIRT6 overexpression accompanied with the inhibition of CGRP and decrease in GLUT proportions. The results also showed that TRPV1 agonist, Capsaicin boosted the SIRT6-induced glucose uptake, CGRP production, and GLUT4 levels. SIGNIFICANCE: Overall, SIRT6 was concluded to be involved in the TRPV1-CGRP-GLUT4 signaling axis thus leading to increased glucose uptake and decreased IR in HFD mice and 3T3-L1 adipocytes. Therefore, in terms of obesity and diabetes, SIRT6 is a novel candidate for treating IR.


Assuntos
Glucose/metabolismo , Resistência à Insulina/fisiologia , Sirtuínas/metabolismo , Células 3T3-L1 , Adipócitos/metabolismo , Animais , Transporte Biológico , Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Dieta Hiperlipídica , Modelos Animais de Doenças , Transportador de Glucose Tipo 4/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Terapia de Alvo Molecular , Obesidade/metabolismo , Canais de Cátion TRPV/metabolismo
10.
Anal Chem ; 91(16): 10657-10662, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31246418

RESUMO

Propyl gallate (PG) as one of the important synthetic antioxidants is widely used in the prevention of oxidative deterioration of oils during processing and storage. Determination of PG has received extensive concern because of its possible toxic effects on human health. Herein, we report a photoelectrochemical (PEC) sensor based on ZnO nanorods and MoS2 flakes with a vertically constructed p-n heterojunction. In this system, the n-type ZnO and p-type MoS2 heterostructures exhibited much better optoelectronic behaviors than their individual materials. Under an open circuit potential (zero potential) and visible light excitation (470 nm), the PEC sensor exhibited extraordinary response for PG determination, as well as excellent anti-inference properties and good reproducibility. The PEC sensor showed a wide linear range from 1.25 × 10-7 to 1.47 × 10-3 mol L-1 with a detection limit as low as 1.2 × 10-8 mol L-1. MoS2/ZnO heterostructure with proper band level between MoS2 and ZnO could make the photogenerated electrons and holes separated more easily, which eventually results in great improvement of sensitivity. On the other hand, formation of a five membered chelating ring structure of Zn(II) with adjacent oxygen atoms of PG played significant roles for selective detection of PG. Moreover, the PEC sensor was successfully used for PG analysis in different samples of edible oils. It demonstrated the ability and reliability of the MoS2/ZnO-based PEC sensor for PG detection in real samples, which is beneficial for food quality monitoring and reducing the risk of overuse of PG in foods.

11.
J Surg Res ; 239: 103-114, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30825755

RESUMO

BACKGROUND: Hemorrhagic shock could induce acute lung injury (ALI), which is associated with cell hypoxia, lung tissue inflammation, free radical damage, and excessive cell apoptosis. Our previous studies demonstrated that hyperoxygenated solution could alleviate cell hypoxia. Furthermore, hydrogen-rich solution (HS) could relieve lung tissue inflammation, free radical damage and excessive cell apoptosis. Therefore we hypothesize that Hyperoxygenated Hydrogen-rich solution (HOHS) can protect the lung against ALI. MATERIALS AND METHODS: SD rats were randomly divided into five groups (n = 6 at each time point in each group) and were exposed to Hemorrhagic shock induced ALI, and then treated with lactated Ringer's solution (LRS), hyperoxygenated solution, HS, and HOHS, respectively. The protective effects of these solutions were assessed using methods as follows: arterial blood samples were collected for blood gas analysis; Bronchoalveolar lavage fluid was collected for cell count and protein quantification; lung tissue samples were collected to measure wet/dry ratio, as well as levels of T-SOD, MDA, TNF-α, and IL-6; Caspase-3 and TUNEL-positive cells, and pathological changes were observed under light microscope; ALI was scored using the Smith scoring method; ultrastructural changes of lung tissues were further observed with transmission electron microscopy. RESULTS: The results indicated that PaO2, PaCO2, and T-SOD increased in the three treatment groups (P < 0.05), most significantly in the HOHS group (P < 0.01) compared with the LRS group; and conversely that the levels of lactate, MDA, TNF-α and IL-6, cell count, protein content, caspase-3 and TUNEL-positive cells as well as ALI score decreased in the three treatment groups (P < 0.05), most significantly in the HOHS group (P < 0.01) compared with the LRS group. Morphological observation with optical microscope and electron microscopy showed that compared with the LRS group, cell damage in the three treatment groups improved to a varying extent, especially evident in the HOHS group. CONCLUSIONS: These findings demonstrate that HOHS can protect the lung against ALI induced by hemorrhagic shock.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Hidratação/métodos , Ressuscitação/métodos , Choque Hemorrágico/complicações , Soluções/administração & dosagem , Lesão Pulmonar Aguda/etiologia , Lesão Pulmonar Aguda/patologia , Administração Oral , Animais , Modelos Animais de Doenças , Humanos , Pulmão/efeitos dos fármacos , Pulmão/patologia , Pulmão/ultraestrutura , Masculino , Microscopia Eletrônica de Transmissão , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Resultado do Tratamento
12.
Brain Imaging Behav ; 13(3): 717-724, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29774500

RESUMO

Premenstrual syndrome (PMS) is a menstrual cycle-related disorder. Although the precise pathophysiology is not fully understood, it is increasingly believed that the central nervous system plays a vital role in the development of PMS. The aim of this study is to elucidate specific functional connectivity between the thalamus and cerebral cortex. Resting-state functional magnetic resonance imaging (fMRI) data were obtained from 20 PMS patients and 21 healthy controls (HCs). Seed-based functional connectivity between the thalamus and six cortical regions of interest, including the prefrontal cortex (PFC), posterior parietal cortex, somatosensory cortex, motor cortex/supplementary motor area, temporal and occipital lobe, was adopted to identify specific thalamocortical connectivity in the two groups. Correlation analysis was then used to examine relationships between the neuroimaging findings and clinical symptoms. Activity in distinct cortical regions correlated with specific sub-regions of the thalamus in the two groups. Comparison between groups exhibited decreased prefrontal-thalamic connectivity and increased posterior parietal-thalamic connectivity in the PMS patients. Within the PMS group, the daily record of severity of problems (DRSP) score negatively correlated with the prefrontal-thalamic connectivity. Our findings may provide preliminary evidence for abnormal thalamocortical connectivity in PMS patients and may contribute to a better understanding of the pathophysiology of PMS.


Assuntos
Vias Neurais/fisiopatologia , Síndrome Pré-Menstrual/fisiopatologia , Mapeamento Encefálico/métodos , Feminino , Giro do Cíngulo/fisiopatologia , Humanos , Imagem por Ressonância Magnética/métodos , Córtex Motor/patologia , Neuroimagem/métodos , Lobo Parietal/fisiopatologia , Córtex Pré-Frontal/fisiopatologia , Síndrome Pré-Menstrual/metabolismo , Tálamo/fisiopatologia , Adulto Jovem
13.
J Cell Physiol ; 234(3): 3020-3028, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30132886

RESUMO

Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide. Mounting evidence showed that circular RNAs (circRNAs) play critical roles in human malignancy. However, the knowledge about circRNAs in GC is still unclear. In the current study, high throughput microarray assay showed that circRBMS3 was upregulated in GC tissues, which was further confirmed by quantitative reverse transcription polymerase chain reaction. Correlation analysis revealed that high circRBMS3 expression was associated with advanced TNM stage, depth of invasion, and lymph-node metastasis. Kaplan-Meier analysis indicated that GC patients with high circRBMS3 expression have a poor overall survival (OS). Function assays showed that circRBMS3 silencing reduced GC cells proliferation and invasion in vitro, and inhibited the tumor growth in vivo. Mechanistically, we found that miR-153 could act as a target of circRBMS3. Subsequently, we showed that circRBMS3 promoted snail family zinc finger 1 (SNAI1) expression via inhibiting miR-153 in GC cells. Collectively, these results suggested that circRBMS3 promoted GC cells proliferation and invasion via regulating miR-153/SNAI1 axis.


Assuntos
MicroRNAs/genética , Fatores de Transcrição da Família Snail/genética , Neoplasias Gástricas/genética , Idoso , Carcinogênese/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Intervalo Livre de Doença , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica/genética , Estadiamento de Neoplasias , Transdução de Sinais
14.
Braz. j. med. biol. res ; 52(1): e7567, 2019. graf
Artigo em Inglês | LILACS-Express | ID: biblio-974265

RESUMO

Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the activity of MMP-2 and MMP-9 were aberrantly increased in SiHa and HeLa cells compared with normal cervical epithelial cells, whereas their activities were strongly inhibited by GPNMB siRNA. Furthermore, Wnt/β-catenin signaling was activated by GPNMB in SiHa and HeLa cells. Increased MMP-2/MMP-9 expression was suppressed by Dkk-1, inhibitor of Wnt/β-catenin signaling, while it was enhanced by stimulator BIO. The proliferation, migration, and apoptosis capacity of HeLa cells were found to be affected by Dkk-1 and BIO to different extents. In conclusion, we demonstrated that GPNMB contributed to the tumorigenesis of cervical cancer, at least in part, by regulating MMP-2/MMP-9 activity in tumor cells via activation of canonical Wnt/β-catenin signaling. This might be a potential therapeutic target for treating human cervical cancer.

15.
Braz J Med Biol Res ; 52(1): e7567, 2018 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-30484490

RESUMO

Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervical cancer. According to the GEO database, we found that GPNMB expression was significantly higher in cervical cancer than in normal cervix epithelium. A similar pattern was observed in GPNMB expression in cultured cervical cancer cells and normal cervical epithelial cells. Compared with the control, GPNMB knockdown significantly decreased the proliferation and migration capacity, but enhanced the apoptosis capacity of SiHa and HeLa cells. Additionally, the activity of MMP-2 and MMP-9 were aberrantly increased in SiHa and HeLa cells compared with normal cervical epithelial cells, whereas their activities were strongly inhibited by GPNMB siRNA. Furthermore, Wnt/ß-catenin signaling was activated by GPNMB in SiHa and HeLa cells. Increased MMP-2/MMP-9 expression was suppressed by Dkk-1, inhibitor of Wnt/ß-catenin signaling, while it was enhanced by stimulator BIO. The proliferation, migration, and apoptosis capacity of HeLa cells were found to be affected by Dkk-1 and BIO to different extents. In conclusion, we demonstrated that GPNMB contributed to the tumorigenesis of cervical cancer, at least in part, by regulating MMP-2/MMP-9 activity in tumor cells via activation of canonical Wnt/ß-catenin signaling. This might be a potential therapeutic target for treating human cervical cancer.


Assuntos
Regulação Neoplásica da Expressão Gênica/genética , Glicoproteínas de Membrana/metabolismo , Neoplasias do Colo do Útero/metabolismo , Via de Sinalização Wnt/genética , beta Catenina/metabolismo , Apoptose , Western Blotting , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Feminino , Humanos , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Glicoproteínas de Membrana/genética , RNA Interferente Pequeno/metabolismo , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/patologia , beta Catenina/genética
16.
Onco Targets Ther ; 11: 6767-6775, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30349309

RESUMO

Purpose: Oxidative stress was significantly associated with the development of malignancies. The purpose of this study was to evaluate the significance of serum total oxidant/antioxidant status in operable advanced gastric cancer patients. Materials and methods: A total of 284 patients who underwent curative resection for primary stage III gastric cancer were enrolled. Total oxidant status, total antioxidant status, and oxidative stress index (OSI) were evaluated within 24 hours before surgery, and compared with 120 healthy donors. The correlation between the OSI and survival outcome was analyzed by the Kaplan-Meier method with log-rank test and Cox's regression methods, respectively. Results: Mean OSI of gastric cancer patients was higher than healthy controls (1.41±0.96 vs 0.78±0.42, P<0.001). All patients were stratified into two groups using the optimal cutoff value (1.42) of OSI using a sensitivity of 94.1% and a specificity of 64.0% as optimal conditions from receiver operating curve analysis. Patients with an OSI ≥1.42 had poorer mean overall survival (45.6 vs 29.8 months, P=0.022) and mean recurrence-free survival (43.3 vs 28.1 months, P=0.011) than patients with an OSI <1.42 in univariate analysis, and OSI was also confirmed as an independent predictor for survival for gastric cancer in multivariate analysis (hazard ratio, 0.541; 95% CI: 0.127-1.102; P=0.01). Conclusion: Preoperative OSI can be considered as an independent prognostic factor for operable and advanced gastric cancer.

17.
Toxins (Basel) ; 10(10)2018 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-30248926

RESUMO

A simple, rapid and efficient methodology was developed and validated for the analysis of four Alternaria toxins in jujube: Tenuazonic acid, alternariol, alternariol monomethyl ether, and tentoxin. Under the optimized extraction procedure, chromatographic conditions, and instrumental parameters, the four toxins were effectively extracted via a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method, and quantified by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Matrix-matched calibrations ranging from 0.01 to 0.5 µg mL-1 were conducted for the quantification due to the matrix effect. A blank jujube sample was spiked at 40, 80 and 160 µg kg-1, obtaining recoveries in the range of 83.5⁻109.6%. Limits of detection and limits of quantification were in the range of 0.14⁻0.26 and 0.47⁻0.87 µg kg-1, respectively. Finally, the developed method was applied for the quantification of the four toxins in 14 jujube samples, including black spot-infected and uninfected samples. Results showed that the predominant toxin detected in all the samples was tenuazonic acid, the content of which was associated with the infection level; alternariol, alternariol monomethyl ether, and tentoxin were detected in all the infected samples and some of the uninfected samples with rather low contents.


Assuntos
Alternaria , Frutas/química , Micotoxinas/análise , Ziziphus/química , Cromatografia Líquida , Contaminação de Alimentos/análise , Frutas/microbiologia , Espectrometria de Massas em Tandem , Ziziphus/microbiologia
18.
Parasit Vectors ; 11(1): 484, 2018 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-30157928

RESUMO

BACKGROUND: Cryptosporidium is a key genus of parasitic protists that infect humans and other vertebrates (mammals and birds). Birds are typically infected with C. avium, C. baileyi, C. galli and/or C. meleagridis, the latter of which is recognised as being zoonotic. Stimulated by the previous finding of C. meleagridis subtypes IIIbA21G1R1, IIIbA22G1R1 and IIIbA26G1R1 in diarrhoeic children in Wuhan city and environs in Hubei Province, China, we performed a molecular epidemiological survey to explore whether these or similar subtypes might occur in farmed chickens in this province. METHODS: PCR-coupled sequencing analyses of regions in the small subunit (SSU) of the nuclear ribosomal RNA and 60 kDa glycoprotein (gp60) genes were utilised to characterise Cryptosporidium in faecal samples from chickens (n = 471) from 14 farms from six distinct regions in Hubei Province. RESULTS: Cryptosporidium baileyi (33/471; 7.0%) and C. meleagridis (15/471; 3.2%) were identified in chickens on eight farms in five of the six distinct geographical regions. No significant age-associated difference in the prevalence of C. baileyi was evident, whereas the prevalence of C. meleagridis was significantly higher in younger (≤ 4 months) than in older chickens (> 4 months). For C. meleagridis, two subtype families, IIIb and IIIe, were defined; some of the subtypes (i.e. IIIbA26G1R1b and IIIbA22G1R1c) characterised here matched those identified previously in diarrhoeic children in Wuhan. CONCLUSIONS: This is the first molecular study reporting the genetic identity and prevalence of C. baileyi and C. meleagridis in chickens in Hubei. The findings suggest that C. meleagridis subtypes IIIbA26G1R1b and IIIbA22G1R1c are cross-transmissible between chickens and humans, raising awareness about the significance of birds as potential reservoirs of zoonotic variants of Cryptosporidium. Future studies might focus on investigating the prevalence of 'zoonotic' subtypes of Cryptosporidium meleagridis in various species of wild and domesticated birds, and on comparing them with those found in humans in China and other countries.


Assuntos
Criptosporidiose/epidemiologia , Cryptosporidium/genética , Cryptosporidium/isolamento & purificação , Doenças das Aves Domésticas/epidemiologia , Zoonoses/epidemiologia , Fatores Etários , Animais , Galinhas/parasitologia , Criança , China/epidemiologia , Criptosporidiose/parasitologia , Criptosporidiose/transmissão , Cryptosporidium/classificação , DNA de Protozoário/genética , Fezes/parasitologia , Genótipo , Humanos , Filogenia , Reação em Cadeia da Polimerase , Doenças das Aves Domésticas/parasitologia , Doenças das Aves Domésticas/transmissão , Prevalência , RNA Ribossômico/genética , Análise de Sequência , Zoonoses/diagnóstico , Zoonoses/parasitologia , Zoonoses/transmissão
19.
Environ Sci Pollut Res Int ; 25(26): 26209-26217, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29974445

RESUMO

Antibiotic resistance genes (ARGs) are regarded as emerging contaminants related with human activities. Aquatic environments of an urban city are apt for the persistence and prevalence of ARGs. In this study, we investigated the occurrence and distribution of ARGs and integrase genes in the sediment samples collected from drinking water sources, urban rivers, and coastal areas of Zhuhai, China, in the dry and wet seasons of 2016. The results show that sulfonamide resistance gene of sulII was present at the highest detection frequency (85.71%); and its average concentrations were also the highest in both dry and wet seasons (3.78 × 107 and 9.04 × 107 copies/g sediment, respectively), followed by tetC, tetO, tetA, ermB, dfrA1, and blaPSE-1. Temporally, the concentrations of total ARGs in the wet season were likely higher than those in the dry season; and spatially, the concentrations of total ARGs in the drinking water sources were substantially lower than those in the urban rivers and nearby coastal areas, indicating the different degrees of anthropogenic impact and consequent health risks. Positive correlations were found between intI1 and each quantitative ARG in all wet season samples rather than dry season samples, which suggested higher temperature and more rain in summer might have positive influences on ARG dissemination, especially that mediated by intI1 gene and class I integrons.


Assuntos
Resistência Microbiana a Medicamentos/genética , Poluentes Ambientais/análise , Genes Bacterianos , Sedimentos Geológicos/análise , Antibacterianos , China , Cidades , Água Potável/análise , Humanos , Integrons , Reação em Cadeia da Polimerase , Rios/química , Estações do Ano , Sulfonamidas
20.
Artif Cells Nanomed Biotechnol ; 46(sup2): 1210-1219, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29961392

RESUMO

MicroRNA (miRNA) plays an important role in tumourigenesis and cancer development by regulating oncogenes or tumour suppressor that are implicated in cell cycle, cell mobility and even cell senescence. Due to the resistance to enzymes that could degrade nucleotides, miRNAs have been considered proper for diagnosis and prognosis evaluation of cancer. The present study was designed to investigate miRNA associated with ESCC and identified effective miRNAs, which could serve as biomarker or targets. We first performed miRNA profiling to identify a subset of dysregulated miRNAs in ESCC. miR-135, miR-451 and miR-186 were the most differentially expressed miRNAs. Subsequent RT-PCR validated that miR-135 was upregulated in ESCC cell lines TE2 and TE9, implying the promise as a prognostic and diagnostic marker. The Cox regression analysis suggests the correlation of miR-135 expression and tumour stages. Survival analysis demonstrated metastatic samples largely have higher miR-135 expression. Downregulation of miR-135 suppressed proliferation and invasion of TE2 and TE9 cell lines. Subsequent target prediction combined with functional enrichment analysis identified "Small GTPase superfamily" that are possibly targeted by miR-135, which offers candidates for further investigation. Finally, RERG was identified as a target of miR-135. Downregulation of RERG could inhibit the cell proliferation and sphere formation ability of TE2 and TE9. Taken together, miR-135 was proved to promote tumour development of ESCC, which promises the prospect of using miR-135 as a biomarker indicator in diagnosis and prognosis.


Assuntos
Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas do Esôfago/genética , Carcinoma de Células Escamosas do Esôfago/patologia , GTP Fosfo-Hidrolases/genética , MicroRNAs/genética , Células-Tronco Neoplásicas/patologia , Carcinoma de Células Escamosas/diagnóstico , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células/genética , Carcinoma de Células Escamosas do Esôfago/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Prognóstico , Regulação para Cima
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