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Langmuir ; 37(1): 339-347, 2021 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-33356306


Targeting delivery is a promising technique for the therapy of cancers. A molecule FA-EEYSV-NH2, which consists of target recognition site folic acid (FA), dipeptide linker, and peptide drug, was designed as a novel anticancer prodrug. The molecules could self-assemble into nanoparticles at pH 7.0 and nanofibers at pH 5.0. By the aid of pH-responsiveness, the self-assemblies were used purposefully as targeted vehicles of self-delivery prodrugs. The results of cell toxicity and internalization assays have proved that the self-assemblies have good cancer cell selectivity. The selection was mainly attributed to the pH-responsive structure transition of self-assemblies and the FA active-targeting effect. We hope that our work could provide a useful strategy for finely tuning the properties and activities of peptide-based supramolecular nanomaterials, thus optimizing nanomedicines with enhanced performance.

Annu Int Conf IEEE Eng Med Biol Soc ; 2020: 1984-1987, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-33018392


Fundus image is commonly used in aiding the diagnosis of ophthalmic diseases. A high-resolution (HR) image is valuable to provide the anatomic information on the eye conditions. Recently, image super-resolution (SR) though learning model has been shown to be an economic yet effective way to satisfy the high demands in the clinical practice. However, the reported methods ignore the mutual dependencies of low-and high-resolution images and did not fully exploit the dependencies between channels. To tackle with the drawbacks, we propose a novel network for fundus image SR, named by Fundus Cascaded Channel-wise Attention Network (FC-CAN). The proposed FCCAN cascades channel attention module and dense module jointly to exploit the semantic interdependencies both frequency and domain information across channels. The channel attention module rescales channel maps in spatial domain, while the dense module preserves the HR components by up- and down-sampling operation. Experimental results demonstrate the superiority of our net-work in comparison with the six methods.

Processamento de Imagem Assistida por Computador , Útero , Atenção , Feminino , Fundo de Olho , Fundo Gástrico
Front Cell Neurosci ; 13: 108, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30949031


Ascorbic acid (AA) is an essential micronutrient that has been safely used in the clinic for many years. The present study indicates that AA has an unexpected function in facilitating nerve regeneration. Using a mouse model of sciatic nerve crush injury, we found that AA can significantly accelerate axonal regrowth in the early stage [3 days post-injury (dpi)], a finding that was revealed by immunostaining and Western blotting for antibodies against GAP-43 and SCG10. On day 28 post-injury, histomorphometric assessments demonstrated that AA treatment increased the density, size, and remyelination of regenerated axons in the injured nerve and alleviated myoatrophy in the gastrocnemius. Moreover, the results from various behavioral tests and electrophysiological assays revealed that nerve injury-derived functional defects in motor and sensory behavior as well as in nerve conduction were significantly attenuated by treatment with AA. The potential mechanisms of AA in nerve regeneration were further explored by investigating the effects of AA on three types of cells involved in this process [neurons, Schwann cells (SCs) and macrophages] through a series of experiments. Overall, the data illustrated that AA treatment in cultured dorsal root ganglionic neurons resulted in increased neurite growth and lower expression of RhoA, which is an important inhibitory factor in neural regeneration. In SCs, proliferation, phagocytosis, and neurotrophin expression were all enhanced by AA. Meanwhile, AA treatment also improved proliferation, migration, phagocytosis, and anti-inflammatory polarization in macrophages. In conclusion, this study demonstrated that treatment with AA can promote the morphological and functional recovery of injured peripheral nerves and that this effect is potentially due to AA's bioeffects on neurons, SCs and macrophages, three of most important types of cells involved in nerve injury and regeneration.

Exp Neurol ; 292: 92-101, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-28283336


Peripheral nerve injury repair can be enhanced by Schwann cell (SC) transplantation, but clinical applications are limited by the lack of a cell source. Thus, alternative systems for generating SCs are desired. Herein, we found the peripheral blood-derived mesenchymal stem cells (PBMSCs) could be induced into SC like cells with expressing SC-specific markers (S100, P75NTR and CNPase) and functional factors (NGF, NT-3, c-Fos, and Krox20). When the induced PBMSCs (iPBMSCs) were transplanted into crushed rat sciatic nerves, they functioned as SCs by wrapping the injured axons and expressing myelin specific marker of MBP. Furthermore, iPBMSCs seeded in an artificial nerve conduit to bridge a 10-mm defect in a sciatic nerve achieved significant nerve regeneration outcomes, including axonal regeneration and remyelination, nerve conduction recovery, and restoration of motor function, and attenuated myoatrophy and neuromuscular junction degeneration in the target muscle. Overall, the data from this study indicated that PBMSCs can transdifferentiate towards SC-like cells and have potential as grafting cells for nerve tissue engineering.

Células-Tronco Mesenquimais/citologia , Regeneração Nervosa/fisiologia , Traumatismos dos Nervos Periféricos/terapia , Recuperação de Função Fisiológica/efeitos dos fármacos , Células de Schwann/citologia , Animais , Axônios/fisiologia , Diferenciação Celular/fisiologia , Células Cultivadas , Bainha de Mielina/metabolismo , Recuperação de Função Fisiológica/fisiologia , Células de Schwann/fisiologia , Transplante de Células-Tronco/métodos , Engenharia Tecidual/métodos , Cicatrização