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1.
ACS Biomater Sci Eng ; 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36054652

RESUMO

Asthma is a common chronic lung disease without absolute treatment, and hypersensitivity reactions and type 2 immune responses are responsible for asthma pathophysiology. ADAM10 as a metalloproteinase transmembrane protein is critical for development of Th2 responses, and levamisole as an anthelmintic drug has immunomodulatory effects, which not only regulates ADAM10 activity but also can suppress the bone marrow and neutrophil production. Therefore, in the present study, nanoparticles were used as a levamisole delivery system to reduce bone marrow suppression, and the immunomodulatory and ADAM10 inhibitory effects of levamisole were studied in allergic asthma. Asthmatic mice were treated with PLGA-levamisole nanoparticles. Then, AHR, BALF, and blood cell counts, levels of the IgG1 subclass, total and OVA-specific IgE, IL2, IL-4, IL-5, IL-10, IL-13, IL-17, IL-25, IL-33, INF-γ, and TNF-α, gene expression of FoxP3, T-bet, RORγt, PU.1, GATA3, FcεRII, CysLT1R, eotaxin, and ADAM10, and lung histopathology were evaluated. PLGA-LMHCl with considered characteristics could control airway hyper-responsiveness, eosinophils in the BALF, levels of immunoglobulins, Th2-, Th9-, and Th17-derived cytokines and pivotal genes, eosinophilic inflammation, hyperplasia of the goblet cell, and hyperproduction of mucus and could increase Th1- and Treg-derived cytokines and also pivotal genes. It could also modulate the ADAM10 activity and had no effect on the number of neutrophils in the bloodstream. The novel safe nanodrug had no side effect on the bone marrow to produce neutrophils and could control the allegro-immuno-inflammatory response of asthma.

2.
Sci Total Environ ; 854: 158801, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36115399

RESUMO

The environmental behavior of arsenic (As) is commonly affected by the biogeochemical processes of iron (Fe) and nitrogen (N). In this study, field experiments were conducted to explore As uptake in rice and As translation and distribution in As-contaminated iron-rich paddy soils after applying different forms of N fertilizers, including urea (CO(NH2)2), ammonium bicarbonate (NH4HCO3), nitrate of potash (KNO3), and ammonium bicarbonate + nitrate of potash (NH4HCO3 + KNO3). The results indicated that applying nitrate N fertilizer inhibited the reduction and dissolution of As-bearing iron minerals and promoted microbial-mediated As(III) oxidation in flooded soil, thus reducing the soil As bioavailability. The concentrations of total As and inorganic As ratio (iAs/TAs) in rice grain decreased by 32.4 % and 15.4 %, respectively. However, the application of ammonium nitrogen promoted the reductive dissolution of As-bearing iron minerals and stimulated microbial As(V) reduction in flooded soil, leading to the release of As from soil to porewater. The total As concentration and inorganic As uptake ratio in rice grain increased by 20.1 % and 6.2 %, respectively, when urea was applied, and by 29.6 % and 10.5 %, respectively, when ammonium bicarbonate was applied. However, the simultaneous application of NH4+ and NO3- had no significant effect on As concentration in rice grain and its transformation in paddy soils. Ammonium nitrogen enhanced the organic As concentration in rice grain because the increased As(III) promoted As methylation in soil. In contrast, nitrate decreased the organic As uptake by rice grain because the decreased As(III) diminished As methylation in soil. The results provide reasonable N fertilization strategies for regulating the As biogeochemical process and reducing the risk of As contamination in rice.

3.
Medicine (Baltimore) ; 101(33): e30119, 2022 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-35984158

RESUMO

To explore the value of ultrasonography in the auxiliary diagnosis of pleural effusion, we retrospectively analyzed the ultrasonographic findings of 275 exudates and 307 transudates and summarized the ultrasonographic image features of pleural effusion according to patients' primary diseases. The findings of thoracic ultrasonography performed before the initial thoracentesis in 582 patients with subsequently confirmed exudative/transudative pleural effusion were analyzed with regard to the sonographic features of pleural effusion. In 275 cases with exudates, thoracic ultrasonography showed a complex septate appearance in 19 cases (6.9%), complex nonseptate appearance in 100 cases (36.4%), complex homogenous sign in 46 cases (16.7%), and pleural thickness > 3 mm in 105 cases. In contrast, in 307 patients with transudates, most patients (97.1%) had bilateral pleural effusion. Ultrasonographic images displayed anechoic appearance and absence of pleural thickening in a vast majority of cases (306, 99.7%; 301, 98%). These positive findings in the exudate were statistically higher than those in their counterparts (P < .05). In the empyema subgroup, the proportion of complex septate appearance, complex nonseptate appearance, complex homogenous sign, and pleural thickening was the highest, at 19/41, 12/41, 10/41, and 30/41, respectively. Ultrasonography is valuable in defining the nature of pleural effusion. Some sonographic features of pleural effusion, such as echogenicity, septation, and pleural thickening, may indicate a high risk of exudative pleural effusion.


Assuntos
Doenças Pleurais , Derrame Pleural , Exsudatos e Transudatos/diagnóstico por imagem , Humanos , Pleura/diagnóstico por imagem , Derrame Pleural/diagnóstico por imagem , Estudos Retrospectivos , Ultrassonografia/métodos
4.
Water Res ; 221: 118804, 2022 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-35797817

RESUMO

Nanoscale zero-valent iron (nZVI) has been extensively used for the passivation of cadmium (Cd) or arsenic (As) from wastewaters, while the underlying mechanisms of nZVI reaction with coexisting Cd and As are largely overlooked. Herein, the interactions of Cd and As during the course of nZVI transformation and the corresponding effects on respective pollutant removal have been systematically investigated. Batch experiments results show that As(III) addition significantly promotes the passivation of Cd(II) by nZVI, and the removal capacity increases by 7.8 times compared to that of Cd(II) alone. However, the adsorption and oxidative transformation of As(III) are barely affected under a relatively low Cd(II) concentration. It is conducive to the adsorption of Cd(II) and As(III) using nZVI under neutral conditions. The transformation of nZVI to lepidocrocite dominates in the Cd(II) single system, while it mainly converts to amorphous Fe oxyhydroxide with the addition of As(III). As(III) notably reduces the surface charge of Fe oxyhydroxide intermediates and to form the ternary complexes with Cd (Fe-As-Cd), which is the predominant mechanism for the promoted Cd(II) passivation. This work provides new understanding of nZVI transformation coupled to Cd(II) and As(III) passivation, which are likely contributing to the heavy metalloids regulation in waters and subsurface environments.


Assuntos
Arsênio , Metais Pesados , Poluentes Químicos da Água , Adsorção , Cádmio , Ferro/análise , Poluentes Químicos da Água/análise
5.
Pathol Res Pract ; 236: 153985, 2022 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-35749916

RESUMO

BACKGROUND: Postmenopausal osteoporosis (PMOP) is the most common bone metabolic disease affecting women worldwide. In this study, we investigate the role of long non-coding RNA (lncRNA) expression in exosomes obtained from bone marrow mesenchymal stem cells (BMSCs) of patients with PMOP. METHODS: BMSCs from patients diagnosed with PMOP and healthy post-menopausal females as controls were isolated and cultured before exosome extraction. RNA microarray technology was used to identify differentially expressed lncRNAs in exosomes from BMSCs. Bioinformatics technology was utilized to analyze the roles of differentially expressed lncRNAs. Further, RT-qPCR was used to validate differentially expressed lncRNAs in 20 pairs of clinical samples. RESULTS: A total of 286 differentially expressed lncRNAs were detected in the exosomes from BMSCs unlike in the control group, among which 148 were up-regulated, whereas 138 were down-regulated. RT-qPCR identified five critical lncRNAs, including ENST00000593078, NR_120593, ENST00000422343, MEG3 and NR_029192. This was consistent with the microarray results and with a significant difference (P < 0.01). Based on the differentially expressed lncRNAs, we constructed lncRNA-miRNA-mRNA interaction networks. Functional analysis revealed that differentially expressed lncRNAs in patients with PMOP potentially target Wnt/ß-catenin, MAPK, and PI3K-Akt pathways. CONCLUSION: In summary, we detected several dysregulated lncRNAs regulating PMOP progression in exosomes extracted from BMSCs of affected patients acting as novel biomarkers. This in turn provides valuable data for targeted treatment of PMOP. SUBJECTS: Genomics; Molecular biology; Orthopedics; Women's Health.

6.
Environ Sci Technol ; 56(13): 9453-9462, 2022 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-35700062

RESUMO

Cocontamination with tetracycline (TC) and arsenic (As) is very common in paddy fields. However, the process and underlying mechanism of arsenite (As(III)) transformation on iron mineral surfaces in the presence of antibiotic contaminants remain unclear. In this study, the release and oxidation of As(III) on ferrihydrite (Fh) surfaces and Fh transformation in the presence of TC under both aerobic and anaerobic conditions were investigated. Our results indicated that the TC-induced reductive dissolution of Fh (Fe(II) release) and TC competitive adsorption significantly promote the release of As, especially under anaerobic conditions. The release of As was increased with increasing TC concentration, whereas it decreased with increasing pH. Interestingly, under both aerobic and anaerobic conditions, the addition of TC enhanced the oxidation of As(III) by Fh and induced the partial transformation of Fh to lepidocrocite. Under aerobic conditions, the adsorbed Fe(II) activated the production of reactive oxygen species (·OH and 1O2) from dissolved O2, with Fe(IV) being responsible for As(III) oxidation. Under anaerobic conditions, the abundant oxygen vacancies of Fh affected the oxidation of As(III) during Fh recrystallization. Thus, this study provided new insights into the role of TC on the migration and transformation of As coupled with Fe in soils.


Assuntos
Arsênio , Antibacterianos , Arsênio/química , Compostos Férricos/química , Compostos Ferrosos , Oxirredução , Oxigênio , Tetraciclina
7.
Water Res ; 219: 118587, 2022 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-35605391

RESUMO

Fe(II) redox chemistry is a pivotal process of biogeochemical Fe cycle and the transformation of organic pollutants in subsurface aquifers, while its interfacial reactivity on iron oxides with varying surface chemistries remains largely unexplored. In this study, the redox processes of Fe(II) on two hematite with highly exposed {001} and {110} facets and their impacts on the transformation of nitrobenzene were investigated. Results suggest that Fe(II) adsorption is the rate-limiting step of the redox chain reactions, controlling the reduction potential (EH). Nitrobenzene activates the facet electron transfer on hematite, leading to nitrobenzene reduction and Fe(II) oxidation. Moreover, {001} facet exhibits a higher reactivity and electron transport efficiency than {110} facet, which is attributed to a lower site density (0.809 #Fe/nm2) and a lower EH of hematite {001} facet than that of {110} facet. It is worth noting that the facet-dependent reduction activity is more intense at low pH or high Fe(II) activity. A slight dissolution of {110} facet was observed, indicating hematite {001} facet exhibits higher thermodynamic stability than {110} facet. This study confirms the facet-dependent reducing activity of surface bound Fe(II) on hematite, providing a new perspective for in-depth understanding of the interfacial reactions on hematite. The findings of this work broaden the biogeochemical process of Fe cycle in subsurface environments and its impact on the fate of organic pollutants in groundwater.


Assuntos
Poluentes Ambientais , Compostos Férricos , Compostos Ferrosos , Nitrobenzenos , Oxirredução
8.
Endocrinol Diabetes Nutr (Engl Ed) ; 69(1): 43-51, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35232559

RESUMO

INTRODUCTION: Elimination or blocking of astrocytes could ameliorate neuropathic pain in animal models. MiR-125a-5p, expressed in astrocyte derived extracellular vesicles, could mediate astrocyte function to regulate neuron communication. However, the role of miR-125a-5p in DPN (diabetic peripheral neuropathy) remains elusive. MATERIALS AND METHODS: Type 2 diabetic mouse (db/db) was used as DPN model, which was confirmed by detection of body weight, blood glucose, mechanical allodynia, thermal hyperalgesia, glial fibrillary acidic protein (GFAP) and monocyte chemoattractant protein-1 (MCP-1). Astrocyte was isolated from db/db mouse and then subjected to high glucose treatment. The expression of miR-125a-5p in db/db mice and high glucose-induced astrocytes was examined by qRT-PCR analysis. Downstream target of miR-125a-5p was clarified by luciferase reporter assay. Tail vein injection of miR-125a-5p mimic into db/db mice was then performed to investigate role of miR-125a-5p on DPN. RESULTS: Type 2 diabetic mice showed higher body weight and blood glucose than normal db/m mice. Thermal hyperalgesia and mechanical allodynia were decreased in db/db mouse compared with db/m mouse, while GFAP and MCP-1 were increased in db/db mouse. High glucose treatment enhanced the protein expression of GFAP and MCP-1 in astrocytes. Sciatic nerve tissues in db/db mice and high glucose-induced astrocytes exhibited a decrease in miR-125a-5p. Systemic administration of miR-125a-5p mimic increased mechanical allodynia and thermal hyperalgesia, whereas it decreased GFAP and MCP-1. TRAF6 (tumor necrosis factor receptor associated factor 6) was validated as target of miR-125a-5p. CONCLUSION: MiR-125a-5p in astrocytes attenuated DPN in db/db mice by up-regulation of TRAF6, which indicated the potential therapeutic effect.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Neuropatias Diabéticas , MicroRNAs , Animais , Astrócitos/metabolismo , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/metabolismo , Neuropatias Diabéticas/genética , Neuropatias Diabéticas/metabolismo , Camundongos , Fator 6 Associado a Receptor de TNF/metabolismo
9.
Am J Transl Res ; 14(1): 501-510, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35173870

RESUMO

OBJECTIVES: Traditional Chinese medicine has been reported to be effective in the treatment of epidemic diseases. Here, we aimed to investigate the effects of combined therapy of Chinese and western medicine on coronavirus disease 2019 (COVID-19). METHODS: A total of 60 patients diagnosed with COVID-19 were enrolled. Both the ordinary and severely affected patients were randomly divided into Groups A-C each with 10 cases each. The patients in Group A-C received Western medicine, Western medicine + traditional Chinese medicine, and Western medicine + traditional Chinese medicine + high dose of vitamin C, respectively. The time of disease recovery, symptoms disappearance, chest CT improvement, and tongue amelioration was recorded. Leukocyte, neutrophil and lymphocyte were monitored, as well as C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), procalitonin (PCT), inflammatory factors, partial pressure of oxygen and carbon dioxide (PaCO2) and oxygenation index (PaO2). Urinary tract stones, liver function, and other side-effects such as gastrointestinal dysfunction were also investigated. RESULTS: Traditional Chinese medicine enhanced the effect of Western medicine, including the reduction of CRP, ESR, PCT, and inflammatory factors, and the increase of leukocyte, neutrophil, and lymphocyte counts, and the improvement of respiratory rate, PaO2, PaCO2, and oxygenation index. Traditional Chinese medicine combined with high-dose Vitamin C therapy more effectively shortened the time of disease recovery, symptom disappearance, chest CT improvement, and tongue amelioration. CONCLUSIONS: a combined therapy of Western medicine, traditional Chinese medicine, and high dose of Vitamin C results in a most effective outcome in the treatment of COVID-19.

10.
Sci Total Environ ; 820: 153306, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-35077783

RESUMO

This work has developed a new strategy of biogeochemical Fe(II) generators for activating microbial Fe(II) generation to immobilize Cd in soils through protons scavenging and coprecipitation. A new biochar modified magnetite (FeBC15) has been fabricated through a top-down method, with which microbial respiration can be stimulated in paddy soil. The FeBC15 exhibits a higher adsorption capacity for Cd than pristine magnetite (1.7 times). The results show that the available Cd can be reduced by 14.4% after adding FeBC15 compared to the control. More importantly, FeBC15 particles promote the conversion of MgCl2 - Cd to stable crystalline Fe/Al bound Cd under the incubation period. The enhanced pH and Fe(II) leads to a comparably lower Cd availability in soils than in pristine soils, which are supported by the enhanced relative abundance of Geobacter and Clostridium with the FeBC15 treatment (i.e. up to 7.44-7.68 × 109 copies/g soil). The Diffusive Gradients in Thin-films (DGT) study indicates that FeBC15 can lower the replenish capacity of soils (i.e. KdL values of 0.2-3.6 mL/g) to soil pore waters and limit root absorption. Pot experiments demonstrate that this strategy can alleviate the rice Cd content by 38.4% (< 0.2 mg/kg). This work paves a new pathway for reducing Cd uptake in rice, enabling sustainable remediation of paddy soil.


Assuntos
Oryza , Poluentes do Solo , Cádmio/análise , Carvão Vegetal/metabolismo , Compostos Ferrosos/metabolismo , Oryza/química , Solo/química , Poluentes do Solo/análise
11.
Brain Res Bull ; 180: 73-85, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-34974133

RESUMO

Extracellular vesicle (EV) from hypoxic adipose tissue-derived mesenchymal stem cells (AD-MSCs) play critical roles in spinal cord injury (SCI) by transferring miRNAs to target cells through fusion with the cell membrane. However, the role of miR-511-3p within the AD-MSCs -derived EV in SCI is largely unknown. Western blotting results demonstrated the secretion of EVs derived from AD-MSCs under hypoxia (Hyp-EVs) was more than those under normoxia (Nor-EVs), and miR-511-3p expression was more enriched in Hyp-EVs. PC12 cells were stimulated with lipopolysaccharide (LPS) to induce cell damage. AD-MSCs were transfected with miR-511-3p mimic or miR-511-3p inhibitor to induce EVs-miR-511-3p overexpression or silencing. Cells treated with Hyp-EVs-miR-511-3p mimic reduced LPS-induced apoptosis, alleviated inflammation and promoted proliferation, while cells treated with Hyp-EVs-miR-511-3p inhibitor aggravated LPS-induced apoptosis and inflammation, and suppressed proliferation. Luciferase reporter gene assay revealed tumor necrosis factor receptor-associated factor 6 (TRAF6) was a target downstream gene of miR-511-3p. A series of gain- and loss-of-function experiments verified that TRAF6 could antagonize the effects of Hyp-EVs-miR-511-3p on inflammation, cell apoptosis and viability. Furthermore, cells treated with CYM5541, an agonist of sphingosine-1-phosphate receptor 3 (S1PR3), reversed the inhibitory effect of Hyp-EVs-miR-511-3p mimic on S1PR3 expression, inflammation and cell apoptosis. Finally, intravenously injection of Hyp-EVs-miR-511-3p mimic into SCI model rats obviously reduced inflammation and promoted neurological function recovery. In conclusion, EVs-derived miR-511-3p from hypoxia preconditioned AD-MSCs ameliorates SCI via TRAF6/S1P/NF-κB pathway, which indicates that miR-511-3p may be a potential therapeutic target for SCI.


Assuntos
Tecido Adiposo/fisiologia , Vesículas Extracelulares , Hipóxia , Células-Tronco Mesenquimais , MicroRNAs/metabolismo , Pró-Proteína Convertases/metabolismo , Serina Endopeptidases/metabolismo , Traumatismos da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/metabolismo , Fator 6 Associado a Receptor de TNF/metabolismo , Animais , Modelos Animais de Doenças , MicroRNAs/farmacologia , Células PC12 , Ratos
12.
Bull Environ Contam Toxicol ; 108(5): 901-908, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35020030

RESUMO

The aim of this study was to identify potentially toxic elements (PTEs) associated with airborne particulate matters (PMs) and their source identification and environmental risk in Isfahan Province, central Iran. Dust samples were collected from various locations included three urban and four rural locations. Results revealed the eastern part of the region as the main source of dust and showed that the highest monthly atmospheric dust deposition was in July (5.53 g m-2). The mean concentrations of Zn, Pb, Cu and Cd were respectively 279, 63, 49 and 0.5 mg kg-1 in dust samples, whereas Cd showed the highest ecological risk index. Dust samples of urban areas showed considerable and very high levels of pollution indices for Pb and Zn, respectively. Among the metals, Zn showed the highest enrichment factor (>5), mainly due to anthropogenic sources. The comprehensive ecological risk index of PTEs revealed the moderate and considerable risk of Isfahan and Najafabad cities, respectively.


Assuntos
Poeira , Metais Pesados , Cádmio , China , Cidades , Poeira/análise , Monitoramento Ambiental/métodos , Irã (Geográfico) , Chumbo , Metais Pesados/análise , Medição de Risco
13.
Sci Total Environ ; 819: 152022, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34856260

RESUMO

The feasibility of chemical stabilization-based strategy for extensive field application is under debate due to lacking a proper framework for its sustainability assessment during its life cycle. Herein, a comprehensive framework consisting of crop production, soil quality, and carbon footprint was constructed for assessing agricultural land remediation based on a two-year paddy field trial. Results show that between the two representative agents, biochar scenario substantially benefits for environmental, social, and agricultural sustainability, because of its more positive impacts on human health and ecosystem, public acceptance, soil reproductive, and rice yield. A notably higher sustainability score of 80.7 for biochar scenario than that of 47.0 for lime is found, in spite of the economical sustainability of lime. The net ecosystem carbon budget of the biochar scenario exhibits an unprecedentedly positive value of 17.8 t CO2-eq ha-1, which can finely contribute to a positive carbon budget during remediation. Our finding demonstrates that biochar strategy enables a multi-objective achievement of soil quality - crop production - carbon budget during agricultural land remediation. This study provides new insights into sustainability assessment for restoring agricultural land for safe crop production and synergizing with carbon neutral plan.


Assuntos
Oryza , Poluentes do Solo , Agricultura/métodos , Cádmio , Carbono , Carvão Vegetal , Ecossistema , Humanos , Solo
14.
J Clin Lab Anal ; 36(1): e23916, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34799880

RESUMO

INTRODUCTION: Bone marrow-derived mesenchymal stem cells (BMSCs)-derived exosomes are involved in the modulation of tissue repair and regeneration. CircRNAs play important roles in BMSCs exosomes. The current study sought to explore the role of circRNAs in exosomes derived from BMSCs of postmenopausal osteoporosis (PMOP) patients and the underlying mechanisms. METHODS: RNA was extracted from BMSCs exosomes of PMOP and a control group. RNA microarray and bioinformatics analyses were used to explore the expression profile and functions circRNAs. Differentially expressed circRNAs from 20 PMOP and 20 controls were analyzed using RT-qPCR. RESULTS: A total of 237 upregulated and 279 downregulated circRNAs were identified in the current study. The top-10 most upregulated circRNAs in the PMOP group were hsa_circ_0069691, hsa_circ_0005678, hsa_circ_0006464, hsa_circ_0015813, hsa_circ_0000511, hsa_circ_0076527, hsa_circ_0009127, hsa_circ_0047285, hsa_circ_0027741, and hsa_circ_0090949. The top-10 most downregulated circRNAs were hsa_circ_0048669, hsa_circ_0090247, hsa_circ_0070899, hsa_circ_0087557, hsa_circ_0045963, hsa_circ_0090180, hsa_circ_0058392, hsa_circ_0040751, hsa_circ_0067910, and hsa_circ_0049484. RT-PCR verified dysregulation of 5 circRNAs including hsa_circ_0009127, hsa_circ_0090759, hsa_circ_0058392, hsa_circ_0090247, and hsa_circ_0049484. Moreover, a circRNA-microRNA-mRNA interaction network was developed based on differentially expressed circRNAs. Functional analysis showed that pathways involved in the regulation of autophagy, PI3K-Akt signaling, FoxO signaling, and MAPK signaling were associated with the differentially expressed circRNAs in PMOP patients. CONCLUSION: The findings of this study show dysregulated circRNAs in BMSCs exosomes of PMOP patients, which may affect the progression of PMOP. These circRNAs can be used as predictive biomarkers and as therapeutic targets for the treatment of PMOP.


Assuntos
Exossomos/genética , Células-Tronco Mesenquimais/citologia , Osteoporose Pós-Menopausa/genética , RNA Circular/genética , Antígenos CD/metabolismo , Estudos de Casos e Controles , Diferenciação Celular/genética , Feminino , Redes Reguladoras de Genes , Humanos , Células-Tronco Mesenquimais/patologia , MicroRNAs/genética , Pessoa de Meia-Idade , Análise de Sequência com Séries de Oligonucleotídeos , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Mensageiro/genética , Reprodutibilidade dos Testes
15.
J Hazard Mater ; 425: 127780, 2022 03 05.
Artigo em Inglês | MEDLINE | ID: mdl-34801297

RESUMO

The redox process of electron shuttles like cysteine on iron minerals under aerobic conditions may largely determine the fate of arsenic (As) in soils, while the interfacial processes and underlying mechanisms are barely explored. This work systematically investigates the interfacial oxidation processes of As(III) on goethite induced by cysteine. Results show that the addition of cysteine significantly enhances the oxidation efficiency (~ 40%) of As(III) (C0: 10 mg/L) by goethite at pH 7 under aerobic conditions, which is 19.5 times of that without cysteine. cysteine induces Fe(III) reduction on the surface of goethite, and the generation absorbed Fe(II) species play an important role in As(III) oxidation. In particular, the further complexation of Fe(II) with cysteine is thermodynamically favorable for electron transfer, leading to an enhanced As(III) oxidation efficiency. The oxidation efficiency of As(III) in the goethite/cysteine system increases by increasing cysteine concentration and decreases by elevating pH conditions. In addition, evidence indicates that •O2- radicals account for approximately 80% of total oxidized As(III). Meanwhile, only 16% of As(III) oxidation can be attributed to the formed •OH radicals. This work provides new insight into the role of organic electron shuttling compounds in determining As cycling in soils.


Assuntos
Arsenitos , Compostos de Ferro , Elétrons , Compostos Férricos , Minerais , Oxirredução , Estresse Oxidativo
16.
J Environ Sci (China) ; 113: 260-268, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34963535

RESUMO

Electron shuttles such cysteine play an important role in Fe cycle and its availability in soils, while the roles of pH and organic ligands in this process are poorly understood. Herein, the reductive dissolution process of goethite by cysteine were explored in the presence of organic ligands. Our results showed that cysteine exhibited a strong reactivity towards goethite - a typical iron minerals in paddy soils with a rate constant ranging from 0.01 to 0.1 hr-1. However, a large portion of Fe(II) appeared to be "structural species" retained on the surface. The decline of pH was favorable to generate more Fe(II) ions and enhancing tendency of Fe(II) release to solution. The decline of generation of Fe(II) by increasing pH was likely to be caused by a lower redox potential and the nature of cysteine pH-dependent adsorption towards goethite. Interestingly, the co-existence of oxalate and citrate ligands also enhanced the rate constant of Fe(II) release from 0.09 to 0.15 hr-1; nevertheless, they negligibly affected the overall generation of Fe(II) in opposition to the pH effect. Further spectroscopic evidence demonstrated that two molecules of cysteine could form disulfide bonds (S-S) to generate cystine through oxidative dehydration, and subsequently, inducing electron transfer from cysteine to the structural Fe(III) on goethite; meanwhile, those organic ligands act as Fe(II) "strippers". The findings of this work provide new insights into the understanding of the different roles of pH and organic ligands on the generation and release of Fe induced by electron shuttles in soils.


Assuntos
Cisteína , Compostos de Ferro , Compostos Férricos , Concentração de Íons de Hidrogênio , Ligantes , Minerais , Oxirredução , Solubilidade
17.
J Hazard Mater ; 424(Pt A): 127361, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34879560

RESUMO

The excessive accumulation of cadmium (Cd) in rice grains is highly determined by the expression of specific genes in different tissues. Targeted gene regulation in rice plants is a long-standing challenge. Herein, a new strategy for regulating target gene expression responsible for Cd absorption and translocation in roots and leaves was developed by complexing Fe(II) with organic matter (i.e., Fe-OM) with the optimal mass ratio of 1. Results showed that Fe-OM noticeably reduced the grain Cd content from 0.48 ± 0.04 mg kg-1 to 0.25 ± 0.03 mg kg-1, exhibiting a significantly higher capacity in mitigating Cd accumulation in grains than Fe(II) or OM alone. The translocation factor (TF) was reduced from 0.14 (control) to 0.08 by Fe-FA from root to grain, which could be due to the preferential Cd translocation to leaves (i.e., TFroot to leaves was enhanced four times by the complex of Fe(II) with fulvic acid (Fe-FA). Further gene analysis revealed that the cooperative effects of OsNramp1 and OsNramp5 downregulation in roots/stems and OsLCT1 upregulation in leaves contributed to the mitigation of Cd in grains. This work provides a new strategy to regulating target gene expression in specific tissues to alleviate Cd accumulation in grains.


Assuntos
Oryza , Poluentes do Solo , Cádmio/análise , Grão Comestível/química , Expressão Gênica , Oryza/genética , Folhas de Planta/química , Folhas de Planta/genética , Solo , Poluentes do Solo/análise
18.
Biomed Res Int ; 2021: 3930800, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34725638

RESUMO

OBJECTIVE: Our study is aimed at investigating the efficacy and safety of Qiwei Tongbi oral liquid in patients with stable long-standing rheumatoid arthritis (RA). METHOD: 140 patients with stable long-standing RA were recruited into the Qiwei Tongbi oral liquid group or the control group. At study recruitment and after 12 weeks of treatment, their C-reactive protein (CRP) levels, interleukin-6 (IL-6) levels, erythrocyte sedimentation rate (ESR), Health Assessment Questionnaire (HAQ), visual analogue scale (VAS), and Disease Activity Score (DAS) 28 were compared in two groups. RESULTS: Patients in the Qiwei Tongbi oral liquid group had a lower level of CRP, IL-6, VAS scale, and HAQ score compared to patients in the control group (CRP: 3.51 ± 1.57 vs.5.47 ± 1.72 mg/L, P < 0.001; IL-6: 1.62 ± 0.8 vs. 2.19 ± 0.88 pg/mL, P < 0.001; VAS scale: 1.59 ± 0.69 vs. 2.66 ± 1.02, P < 0.001; and HAQ score: 1.19 ± 0.46 vs. 1.41 ± 0.50, P = 0.005). The ESR and DAS28 did not reach statistical difference. No damage to liver and kidney functions was observed in both groups. CONCLUSION: Qiwei Tongbi oral liquid has the tendency to decrease the inflammation levels and pain score and improve patients' outcomes in patients with stable long-standing RA.


Assuntos
Artrite Reumatoide/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Inflamação/tratamento farmacológico , Idoso , Idoso de 80 Anos ou mais , Antirreumáticos/uso terapêutico , China , Feminino , Humanos , Masculino , Medicina Tradicional Chinesa/métodos , Pessoa de Meia-Idade , Medição da Dor , Inquéritos e Questionários , Resultado do Tratamento
19.
Artigo em Inglês | MEDLINE | ID: mdl-34666620

RESUMO

In this study, 321 chestnut samples from Shandong Province in China were analysed for the presence of mycotoxins. We screened for 14 mycotoxins including aflatoxins (AFs: AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), T-2 toxin (T-2), zearalenone (ZEA), ochratoxin A (OTA), fumonisins (FB1, FB2, FB3), and penicillic acid (PeA). Mycotoxins were detected in 56.4% of the samples, and 11 of these mycotoxins were found. Thirty samples from the Shandong Province markets were deemed positive for AFs (9.3%) and had an AFB1 level of >2 µg/kg or a sum of AFB1, AFB2, AFG1, and AFG2 that was >4 µg/kg, which exceed the maximum tolerable level of the European regulations standards (EC/188/2006). The contamination level for total mycotoxins found in chestnuts was in the range of 0.6-2,791.0 µg/kg. The estimated daily intake (EDI) values for each individual mycotoxin and for all of the mycotoxins collectively were calculated by both a deterministic approach and a probabilistic approach. For risk characterisation, dietary exposure to DON, ZEA, FBs, and OTA through consumption of chestnuts, analysed according to both approaches, showed no health risk to Chinese adolescents and adults from exposure to either individual mycotoxins or in combination, but more concern should be paid to the AFs for adolescents and adults at a high consumption level. This is believed to be the first work performing risk assessment of multiple mycotoxins specifically for adolescents, including the recently isolated FBs and PeA, which have recently emerged as mycotoxins of concern, in chestnuts of Shandong Province in China.


Assuntos
Exposição Dietética/análise , Análise de Alimentos , Contaminação de Alimentos/análise , Micotoxinas/análise , Nozes/química , Adolescente , Criança , China , Humanos , Medição de Risco
20.
Huan Jing Ke Xue ; 42(9): 4520-4526, 2021 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-34414752

RESUMO

Nanoscale zero-valent iron (nZVI) shows excellent reduction of Cr(Ⅵ), but the passivation on its outer surface can restrict its longevity and performance. To tackle this problem, this work introduced Shewanella oneidensis MR-1, a dissimilatory iron-reducing bacterium, into the chemical reduction system of aged nZVI/biochar (B) and Cr(Ⅵ). The potential synergistic effect of Cr(Ⅵ) reduction of aged nZVI/B and MR-1 was systematically investigated under varying conditions. The results indicated that aged nZVI/B and MR-1 exhibited a synergistic effect at a pH of 7, and the removal rate of Cr(Ⅵ) increased by 51.3%. Further research showed that the synergistic effect could be attenuated with the increase in the initial Cr(Ⅵ) concentration and enhanced with the increase in the MR-1 concentration. The XPS spectra confirmed that Cr(Ⅵ) was mainly removed through reduction. The dissimilatory iron-reducing ability of MR-1 played a key role in enhancing the Cr(Ⅵ) reduction. The reductive dissolution of the oxidation layers not only released reactive sites inside the nZVI, but also reduced Cr(Ⅵ) by producing ferrous ions. Moreover, B promoted the reduction by dispersing the nZVI and mediating the extracellular electron transfer. This study provides a new insight into solving the passivation problem of the long-term application of nZVI for Cr(Ⅵ) removal, which is considered a promising solution for synergistically improving the performance of nZVI in environmental remediation.


Assuntos
Ferro , Poluentes Químicos da Água , Carvão Vegetal , Cromo , Shewanella , Poluentes Químicos da Água/análise
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