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1.
Food Sci Biotechnol ; 30(10): 1293-1301, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34721925

RESUMO

Tea is one of the most widely consumed aromatic beverages in the world because of its taste and flavor, as well as due to many potential health beneficial properties. Metabolomics focuses on an in-depth analysis of all metabolites in living organisms. In this study, 29 primary metabolites and 25 secondary metabolites were identified using GC/MS and UPLC-QTOF/MS, respectively. Further, PCA analysis showed conspicuous discrimination for the ten varieties of green tea with metabolite profiling. Among them, organic acids, amino acids, flavan-3-ols, and flavonol glycosides varied greatly through checking the VIP values of the PLS-DA model. Moreover, the intrinsic and/or extrinsic factors characterizing each type of green tea were also discussed. The chemical component marker derived here should be used as an important detection index, while evaluating the tea quality, as well as while establishing the tea quality standard. Supplementary Information: The online version contains supplementary material available at 10.1007/s10068-021-00970-4.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34727037

RESUMO

Intrinsic disorder is common in proteins, plays important roles in protein functionality, and is commonly associated with various human diseases. To have an accurate tool for the annotation of intrinsic disorder in proteins, this paper proposes a novel algorithm, DeepCLD, for sequence-based prediction of intrinsically disordered proteins. This algorithm uses amino acid position specific scoring matrix (PSSM) to capture the intrinsic variability characteristic of sequence patterns, ResNet to preserve feature space structure, and bidirectional CudnnLSTM as recurrent layer to further improve the efficiency. Futhermore, DeepCLD also utilized the attention mechanism to solve the problem of gradient disappearing in deep network. Comparative analyses show that DeepCLD has faster training speed and higher prediction accuracy than comparable methods.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34734347

RESUMO

Microbiological confirmation is rare in children with active tuberculosis; therefore, a more accurate test is needed to detect pulmonary tuberculosis in children. In this multicenter study, we evaluated the utility of the Xpert MTB/RIF Ultra (Ultra) on sputum, an assay recommended by the World Health Organization to test for childhood tuberculosis in high-burden settings. Children with symptoms suggestive of tuberculosis were enrolled at three hospitals in China and categorized as having active tuberculosis or nontuberculosis. The sensitivity and specificity of Ultra were 42.1% (48/114) and 99.0% (208/210), respectively. Using three MTB culture results as the reference, the sensitivity of Ultra in the subset of 38 children with culture-positive and 76 children with culture-negative was 68.4% (26/38) and 28.9% (22/76), respectively(p < 0.001). A single MTB culture combined with a single Ultra could detect 54 (54/114,47.4%) cases with active TB, while repeated MTB culture combined with a single Ultra detected 60 (60/114, 52.6%) cases with active TB(p = 0.427). Among 155 children (58 with TB and 97 with RTIs) simultaneously tested with the Ultra and Xpert MTB/RIF (Xpert), the sensitivity of the Xpert (24.1%, 14/58) was lower than that of the Ultra (41.4%, 24/58; p = 0.048). Eight children were found to have rifampin-resistant MTB strains. The Xpert MTB/RIF Ultra assay should be implemented to test for pulmonary tuberculosis in children to achieve higher confirmation rates.

4.
Int J Infect Dis ; 2021 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-34774781

RESUMO

OBJECTIVES: We analyzed the performance of Xpert MTB/RIF Ultra (Ultra) on stool and gastric aspirate (GA) samples for the diagnosis of pediatric pulmonary tuberculosis (TB) caused by Mycobacterium tuberculosis in a high burden area in China. METHODS: Children with presumptive TB were enrolled in two hospitals in Sichuan Province (July 2019-Oct 2020). Because of the unavailable sputum for etiological tests, GA samples were aspirated and tested by bacterial culture, acid-fast bacillus microscopy and Ultra. Stool samples were tested simultaneously using Ultra and Xpert. RESULTS: Finally, 141 children with active TB and 34 with non-TB respiratory tract infections were enrolled. Ultra-stool (60.3%, 85/141) and Ultra-GA (52.5%, 74/141) were similarly sensitive (P=0.187). Among the subset of 48 children with confirmed TB, Ultra testing was equally sensitive on stool and GA samples (85.4%, 41/48). The agreement between Ultra-stool and Ultra-GA was moderate in children with active TB (kappa value = 0.527). After integrating Ultra-GA and Ultra-stool outcomes, 70.9% (100/141) of the children were considered to have confirmed TB. The specificities of Ultra-stool and Ultra-GA were 97.1% (33/34) and 100% (34/34), respectively (P=0.314). CONCLUSIONS: In children, stools can be used as alternative samples to GAs for Ultra tests. Stool- and GA-based Ultra are appropriate tests for bacteriological TB confirmation.

5.
Appl Environ Microbiol ; : AEM0192121, 2021 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-34818106

RESUMO

Legionella pneumophila (LP) widely exists in natural and artificial water environments, which facilitates LP to infect people. LP infection causes Legionnaires' disease (LD), which is an important but relatively uncommon respiratory infection. Approximately 90% of LD is caused by L. pneumophila serogroup 1 (Lp1). Meteorological conditions may affect the infectivity and virulence of Lp1, but the exact relationship between them is still unclear. In this study, we evaluated the virulence of Lp1 by screening of total 156 Lp1 strains isolated from cooling tower water in different region of China by detecting their abilities to activate NF-κB signaling pathway in vitro. In addition, we screened the distribution of some selected virulence genes in these strains. The virulence, virulence gene distribution and the meteorological factors were analyzed. We found that both the virulence and the distribution of virulence genes had a certain regional and meteorological correlation. Although loss of several virulence genes showed significant effects on the virulence of Lp1 strains, the distribution of virulence genes had very limited effects on the virulence of Lp1. IMPORTANCE LD is likely to be under-recognized in many countries. Due to the widespread existence of LP in natural and artificial water environments, and to the lack of cross-protection against different strains, LP is a potentially serious threat to human health. Therefore, effective monitoring of the virulence of LP in the water environment is very important to prevent and control the prevalence of LD. Understanding the virulence of LP can not only help us to predict the risk of possible outbreaks in advance, but can also enable more targeted clinical treatment. This study highlights the importance of understanding the epidemiology and ecology of LP isolated from public facilities in terms of public health and biology. Due to the potential for water sources to harbor and disseminate LP, and to the fact that geographical conditions influence the virulence of LP, timely and accurate LP virulence surveillance is urgently needed.

6.
Trials ; 22(1): 774, 2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34742345

RESUMO

BACKGROUND: Insomnia is a common sleeping disorder which affects the quality of life which can bring harms to physical and mental health of human beings and even economic development. Traditional Chinese medicine (TCM) plays an outstanding role in treating chronic diseases and alleviating their symptoms. Therefore, the purpose of this study is to assess the treatment efficacy in patients with insomnia treated with abdomen-rubbing qigong exercise (ARQE). In addition, the brain function changes of patients will be explored by resting state functional magnetic resonance imaging (rs-fMRI). METHOD/DESIGN: This trial is a randomized, single-blind, controlled study planned to transpire between July 1, 2020, and July 31, 2021. A sample size of 114 participants (57 per group) with chronic insomnia will be randomly assigned to receive ARQE or CBTI for 8 weeks. The study duration will be 13 weeks, including a 1-week screening period, 8 weeks of intervention, and another 4 weeks of follow-up. The primary outcome is the Pittsburgh sleep quality index scores. Secondary outcomes include insomnia severity index, gastrointestinal symptom rating scale, the Hamilton Depression Scale, and rs-fMRI scan. The adverse events will be in control. DISCUSSION: The results of this study will help to clarify the efficacy of ARQE in the treatment of insomnia and try to use rs-fMRI technology to explore the brain function changes of ARQE in improving sleep quality in patients with insomnia disorder. If the results are as expected, this study will provide high-quality evidence for the treatment of insomnia with ARQE. TRIAL REGISTRATION: China Clinical Registration Agency ChiCTR1900028009 . Registered on 19 December 2019.


Assuntos
Qigong , Distúrbios do Início e da Manutenção do Sono , Abdome , Humanos , Qualidade de Vida , Ensaios Clínicos Controlados Aleatórios como Assunto , Método Simples-Cego , Distúrbios do Início e da Manutenção do Sono/diagnóstico , Distúrbios do Início e da Manutenção do Sono/terapia , Resultado do Tratamento
7.
Ying Yong Sheng Tai Xue Bao ; 32(9): 3185-3194, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34658204

RESUMO

Winter wheat is one of the main crops in Anhui Province, to analyze the spatial-temporal variations of climate-induced reduction risk is of great significance to ensure regional food security. Based on the data of winter wheat yield from 1973 to 2014 in 74 districts and counties, nine risk assessment indicators were extracted from annual series of climate-induced reduction rate, and the spatial-temporal variations of climate-induced reduction risk for winter wheat in Anhui was analyzed by using principal component analysis. The results showed that north Anhui, especially the region along Huaihe River, was the higher-risk area for climate-induced yield loss. Results of the barycenter migration model showed that the high value regions of climate-induced reduction rate of winter wheat in Anhui Province had a significant tendency of moving from north to south. In the whole province, climate-induced reduction risk of winter wheat presented a significant interdecadal variation. Furthermore, the number of districts and counties with moderate or more reduction intensity was significantly reducing since the 2000s. Results from S-mode principal component analysis and climate-induced reduction rate series showed that the climate-induced reduction risk for winter wheat in north Anhui had a downward trend and that in southern Anhui had an upward trend. Climate-induced reduction risk of winter wheat in Anhui had notable temporal and spatial variations, the interdecadal fluctuation and north-south regional difference of which should draw our attention.


Assuntos
Clima , Triticum , Produtos Agrícolas , Análise de Componente Principal
8.
Exp Ther Med ; 22(6): 1364, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34659510

RESUMO

Chemoresistance is the primary cause of the poor outcome of glioblastoma multiforme (GBM) therapy. Leucine-rich repeat-containing G-protein coupled receptor 6 (LGR6) is involved in the growth and proliferation of several types of cancer, including gastric cancer and ovarian cancer. Therefore, the aim of the present study was to investigate the role of LGR6 in GBM malignancy and chemoresistance. Cell counting kit-8 and Matrigel®-Transwell assays were conducted to assess GBM cell viability and invasion. The effect of LGR6 on cell cycle progression and activation of Akt signaling was analyzed by performing propidium iodide staining and western blotting, respectively. The results demonstrated that LGR6, a microRNA-1236-3p target candidate, promoted GBM cell viability and invasion, and mediated temozolomide sensitivity in SHG-44 and U251 GBM cells. In addition, LGR6 triggered the activation of the Akt signaling pathway during GBM progression. Collectively, the results of the present study suggested that LGR6 promoted GBM malignancy and chemoresistance, at least in part, by activating the Akt signaling pathway. The results may aid with the identification of a novel therapeutic target and strategy for GBM.

9.
BMC Genomics ; 22(1): 750, 2021 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-34663228

RESUMO

BACKGROUND: Chloroplast transfer RNAs (tRNAs) can participate in various vital processes. Gymnosperms have important ecological and economic value, and they are the dominant species in forest ecosystems in the Northern Hemisphere. However, the evolution and structural changes in chloroplast tRNAs in gymnosperms remain largely unclear. RESULTS: In this study, we determined the nucleotide evolution, phylogenetic relationships, and structural variations in 1779 chloroplast tRNAs in gymnosperms. The numbers and types of tRNA genes present in the chloroplast genomes of different gymnosperms did not differ greatly, where the average number of tRNAs was 33 and the frequencies of occurrence for various types of tRNAs were generally consistent. Nearly half of the anticodons were absent. Molecular sequence variation analysis identified the conserved secondary structures of tRNAs. About a quarter of the tRNA genes were found to contain precoded 3' CCA tails. A few tRNAs have undergone novel structural changes that are closely related to their minimum free energy, and these structural changes affect the stability of the tRNAs. Phylogenetic analysis showed that tRNAs have evolved from multiple common ancestors. The transition rate was higher than the transversion rate in gymnosperm chloroplast tRNAs. More loss events than duplication events have occurred in gymnosperm chloroplast tRNAs during their evolutionary process. CONCLUSIONS: These findings provide novel insights into the molecular evolution and biological characteristics of chloroplast tRNAs in gymnosperms.


Assuntos
Cycadopsida , Ecossistema , Cloroplastos/genética , Cycadopsida/genética , Filogenia , RNA de Transferência/genética
10.
ACS Appl Mater Interfaces ; 13(40): 47465-47477, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34592812

RESUMO

Regarding dihydrogen as a clean and renewable energy source, ammonia borane (NH3BH3, AB) was considered as a chemical H2-storage and H2-delivery material due to its high storage capacity of dihydrogen (19.6 wt %) and stability at room temperature. To advance the development of efficient and recyclable catalysts for hydrolytic dehydrogenation of AB with parallel insight into the reaction mechanism, herein, ZIF-67-derived fcc-Co@porous carbon nano/microparticles (cZIF-67_nm/cZIF-67_µm) were explored to promote catalytic dehydrogenation of AB and generation of H2(g). According to kinetic and computational studies, zero-order dependence on the concentration of AB, first-order dependence on the concentration of cZIF-67_nm (or cZIF-67_µm), and a kinetic isotope effect value of 2.45 (or 2.64) for H2O/D2O identify the Co-catalyzed cleavage of the H-OH bond, instead of the H-BH2NH3 bond, as the rate-determining step in the hydrolytic dehydrogenation of AB. Despite the absent evolution of H2(g) in the reaction of cZIF-67 and AB in the organic solvents (i.e., THF or CH3OH) or in the reaction of cZIF-67 and water, Co-mediated activation of AB and formation of a Co-H intermediate were evidenced by theoretical calculation, infrared spectroscopy in combination with an isotope-labeling experiment, and reactivity study toward CO2-to-formate/H2O-to-H2 conversion. Moreover, the computational study discovers a synergistic interaction between AB and the water cluster (H2O)9 on fcc-Co, which shifts the splitting of water into an exergonic process and lowers the thermodynamic barrier for the generation and desorption of H2(g) from the Co-H intermediates. With the kinetic and mechanistic study of ZIF-67-derived Co@porous carbon for catalytic hydrolysis of AB, the spatiotemporal control on the generation of H2(g) for the treatment of inflammatory diseases will be further investigated in the near future.

11.
Mult Scler Relat Disord ; 56: 103314, 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34634624

RESUMO

OBJECTIVE: To determine the longitudinal changes in retinal microstructure, microvasculature, microcirculation, and axonal and neuronal functions in patients with relapsing-remitting multiple sclerosis (RRMS) over the time course of about two years. METHODS: A total of 30 patients (60 eyes) with RRMS were followed for a period of 27 ± 6 months and evaluated with a battery of clinical tests including low contrast letter acuity (LCLA), intraretinal layer thicknesses by optical coherence tomography (OCT), ganglion cell function by steady-state pattern electroretinography (PERG), axonal function by polarization-sensitive OCT, volumetric vessel density (VVD) by OCT angiography, and retinal tissue perfusion (RTP) by retinal function imager. RESULTS: Axonal function measured as retinal nerve fiber layer birefringence in the temporal quadrant and vessel density in the deep vascular plexus were significantly decreased at 2-year follow-up (P < 0.05). Subgroup analyses showed that the increased retinal blood flow volume occurred in patients with no evidence of disease activity (NEDA), and with stable or improved visual function (P < 0.05). There was no significant difference in the expanded disability state scale, LCLA, RTP, VVD, or PERG measures between the two visits (P > 0.05). CONCLUSION: To our best knowledge, this is the first 2-year prospective comprehensive study with a detailed assessment of retinal microstructure and neuronal functions in patients with RRMS. The recovery of retinal microcirculation occurred in patients with NEDA, and stable or improved visual function, suggesting these measurements as potential imaging biomarkers for monitoring disease progression.

12.
Integr Med Res ; 10: 100798, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34692409

RESUMO

Background: We aimed to investigate use of infection control behaviours, preventative and therapeutic interventions, and outcomes among respondents to an online survey during the COVID-19 pandemic in China. Methods: The survey was designed by an international team, translated and adapted to simplified Chinese, including 132 kinds of traditional Chinese medicine (TCM) preparation recommended by guidelines. It was distributed and collected from February to May 2021, with data analysed by WPS spreadsheet and wjx.cn. Descriptive statistics were used to describe demographics and clinical characteristics, diagnosis, treatments, preventative behaviours and interventions, and their associated outcomes. Results: The survey was accessed 503 times with 341 (67.8%) completions covering 23 provinces and four municipalities in China. Most (282/341, 82.7%) respondents reported no symptoms during the pandemic and the majority (290/341, 85.0%) reported having a SARS-CoV-2 PCR test at some point. Forty-five (13.2%) reported having a respiratory infection, among which 19 (42.2%) took one or more categories of modern medicine, e.g. painkillers, antibiotics; 16 (35.6%) used TCM interventions(s); while seven respondents combined TCM with modern medicine. All respondents reported using at least one behavioural or medical approach to prevention, with 22.3% taking TCM and 5.3% taking modern medicines. No respondents reported having a critical condition related to COVID-19. Conclusion: We found evidence of widespread use of infection control behaviours, modern medicines and TCM for treatment and prevention of COVID-19 and other respiratory symptoms. Larger scale studies are warranted, including a more representative sample exploring TCM preparations recommended in clinical guidelines.

13.
Aging (Albany NY) ; 13(17): 21628-21641, 2021 09 10.
Artigo em Inglês | MEDLINE | ID: mdl-34506303

RESUMO

INTRODUCTION: This multicenter, retrospective study assessed the prevalence of post-stroke cognitive impairment (PSCI) 6 months after acute ischemic stroke (AIS) and its risk factors to build a bedside early predictive model for PSCI using the Montreal Cognitive Assessment (MoCA). METHODS: Records of consecutive patients with AIS treated at 4 stroke centers in Shanghai had MoCA assessments within 2 weeks after AIS onset and 6 months later were reviewed. Prevalence of PSCI (MoCA<22) was calculated and risk factors were identified by multivariate logistic regression analysis. The modeling and validation and identified risk factors were included in a predictive model using multivariate regression. RESULTS: There were 383 patients included and prevalence of PSCI 6 months after AIS was 34.2%, significantly lower than prevalence of patients with acute cognitive impairment (49.6%). Aging, less education, higher glucose level and severe stroke were PSCI risk factors, while level of low-density lipoprotein cholesterol (LDL-C) had a paradox effect on the risk of PSCI. 40.0% of the patients with cognitive impairment at acute phase reverted to normal, and patients with LDL-C 1.8-2.5 mmol/L were more likely to revert. The predictive model we built, DREAM-LDL (Diabetes [fasting blood glucose level], Rating [NIHSS], level of Education, Age, baseline MoCA and LDL-C level), had an AUROC of 0.93 for predicting PSCI at 6 months. CONCLUSION: PSCI was common among AIS patients 6 months after AIS. We provided a practical tool to predict PSCI based on MoCA and risk factors present during acute phase of AIS.

14.
Polymers (Basel) ; 13(18)2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34577944

RESUMO

In this study, a micro-molding technology was used to prepare the microneedles (MNs), while a texture analyzer was used to measure its Young's modulus, Poisson's ratio and compression breaking force, to evaluate whether the MNs can penetrate the skin. The effects of different materials were characterized by their ability to withstand stresses using the Structural Mechanics Module of COMSOL Multiphysics. Carboxymethylcellulose (CMC) was chosen as the needle formulation material with varying quantities of polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA) and hyaluronic acid (HA) to adjust the viscosity, brittleness, hardness and solubility of the material. The results of both the experimental tests and the predictions indicated that the hardest tip material had a solids content of 15% (w/w ) with a 1:2 (w/w) CMC: HA ratio. Furthermore, it was shown that a solid content of 10% (w/w) with a 1:5 (w/w) CMC: PVA ratio is suitable for making patches. The correlation between the mechanical properties and the different materials was found using the simulation analysis as well as the force required for different dissolving microneedles (DMNs) to penetrate the skin, which significantly promoted the research progress of microneedle transdermal drug delivery.

15.
Res Sports Med ; 29(6): 586-592, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34477036

RESUMO

This study examined the biomechanics of the lower limbs during four typical Tai Chi (TC) movements: wave hand in cloud, leaning fly side, repulse monkey, and brush knee and twist step, in order to provide biomechanical evidence-based recommendations for patients with knee osteoarthritis (OA) practicing TC. Joint angles and joint moments of the hip, knee, and ankle in frontal and sagittal plane as well as ground reaction forces were examined while performing TC and regular walking in an experienced 38 years old TC master. The results showed that relative to walking, the four TC movements are characterized by a wide motion range of lower limbs, slow increase in joint loading, and strong muscle activity during performance. Therefore, these TC movements could be suitable for patients with knee OA to practice for improving the muscle strength of their lower limbs and functional ability.


Assuntos
Extremidade Inferior/fisiologia , Osteoartrite do Joelho/fisiopatologia , Osteoartrite do Joelho/reabilitação , Amplitude de Movimento Articular/fisiologia , Tai Ji/métodos , Caminhada/fisiologia , Suporte de Carga/fisiologia , Adulto , Fenômenos Biomecânicos , Humanos , Cinética , Masculino
16.
Se Pu ; 39(9): 941-949, 2021 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-34486833

RESUMO

Sample pretreatment technology plays a vital role throughout the analysis of complex samples. Sample pretreatment can not only increase the concentration of trace targets in the sample, but also effectively eliminate interference from the sample matrix in instrumental analysis. Adsorbent materials are a key component of sample pretreatment technology. Therefore, the development of efficient and stable new adsorbent materials has acquired significance in research on pretreatment technology. Porous materials are advantageous for use in diverse applications, such as in adsorbents, when they possess controllable nanostructures, a tailored pore surface chemistry, and abundant porosity, and are inexpensive. Particularly in recent years, porous materials derived from metal-organic frameworks (MOFs) feature excellent properties, such as diverse morphology and structure, adjustable pore size, high specific surface area, good thermal stability, and chemical resistance. MOF-derived materials, when used as adsorbents for sample pretreatment, offer the following advantages: (1) The porous materials derived from MOFs typically possess a larger specific surface area than other porous materials. This characteristic is beneficial to improve the extraction capacity and extraction efficiency via an increase in the contact area between the materials and targets; (2) The microscopic porous structure of MOF-derived materials can be easily tuned (by controlling the temperature and time during pyrolysis, gas atmosphere, and heating rate), which is conducive to improve the selectivity of sample pretreatment methods; (3) The metal active sites can be evenly distributed. Owing to the ordered distribution of metal ions in the precursor MOFs and a good periodic framework structure, the metal active sites of the derivatives formed can still maintain a corresponding distance. These metal active sites will not form agglomerates and affect the extraction performance; conversely, other porous materials often require extremely complicated processes to achieve a uniform distribution; (4) Heteroatoms such as nitrogen and sulfur can be easily doped on the framework of MOF-derived porous materials. This doping enables the materials to induce additional interactions such as hydrogen bonding and π-π stacking for adsorbing target analytes. The excellent properties of MOF-derived materials make them promising for use in sample pretreatment. Novel sample pretreatment methods that use MOF-derived materials are constantly being developed. However, the use of MOF-derived materials is limited by the complex preparation process and high production cost of MOF precursors, along with difficulties in mass production. Further, the precise design or functionalization of MOF-derived materials according to the characteristics of targets is a new direction with immense challenges as well as application potential. This review summarizes the application of MOF-derived materials in sample pretreatment methods, including dispersive solid phase extraction (dSPE), magnetic solid phase extraction (MSPE), solid phase microextraction (SPME), stir bar sorptive extraction (SBSE), and dispersive micro solid phase extraction (DMSPE). The preparation methods, functional control, and enrichment efficiencies of various MOF-derived materials are also reviewed. Finally, the application prospects of MOF-derived materials in sample pretreatment are discussed to provide a clear outlook and reference for further related research.


Assuntos
Estruturas Metalorgânicas , Íons , Metais , Extração em Fase Sólida , Microextração em Fase Sólida
17.
Sci Rep ; 11(1): 17746, 2021 09 07.
Artigo em Inglês | MEDLINE | ID: mdl-34493780

RESUMO

Bullous pemphigoid (BP), the most frequent blistering dermatosis in the elderly, is associated with increased mortality. The severity of BP can be assessed by detecting the anti-BP180 immunoglobulin G (IgG) concentration, but the lab test is not available in many community clinics. BP patients are usually in a hypercoagulable state with increased levels of D-dimer and fibrin degradation products (FDPs). We aimed to evaluate the use of D-dimer and FDPs in assessing BP severity. We compared the levels of plasma D-dimer, plasma FDPs, eosinophil counts, eosinophil cationic protein, and serum anti-BP180 IgG concentration between 48 typical BP patients and 33 Herpes zoster (HZ) patients (control group). Correlational analyses were conducted to determine the relationships between the lab values and common BP severity markers. The plasma D-dimer and FDP levels were higher in BP patients than in HZ controls (D-dimer: 3297 ± 2517 µg/L vs. 569.70 ± 412.40 µg/L; FDP: 9.74 ± 5.88 mg/L vs. 2.02 ± 1.69 mg/L, respectively, P < 0.0001). Significant positive correlations were found between D-dimer/FDP levels and BP severity markers (i.e. anti-BP180 IgG concentration [D-dimer: r = 0.3928, P = 0.0058; FDP: r = 0.4379, P = 0.0019] and eosinophil counts [D-dimer: r = 0.3625, P = 0.0013; FDP: r = 0.2880, P = 0.0472]) in BP patients. We also found an association between FDP and urticaria/erythema lesions (r = 0.3016, P = 0.0372), but no other BPDAI components. In 19 BP patients with complete remission after systemic glucocorticoid treatment, D-dimer and FDP levels decreased post-therapy (D-dimer: 5559 ± 7492 µg/L vs. 1738 ± 1478 µg/L; P < 0.0001; FDP: 11.20 ± 5.88 mg/L vs. 5.13 ± 3.44 mg/L; P = 0.0003), whereas they did not in BP patients with treatment resistant. Plasma D-dimer and FDP are convenient markers to evaluate BP severity assistant on BPDAI and eosinophil counts. FDP is also helpful for inflammatory lesions in BP patients.


Assuntos
Produtos de Degradação da Fibrina e do Fibrinogênio/análise , Penfigoide Bolhoso/sangue , Idoso , Idoso de 80 Anos ou mais , Autoanticorpos/sangue , Autoantígenos/imunologia , Biomarcadores , Estudos Transversais , Proteína Catiônica de Eosinófilo/sangue , Eosinofilia/sangue , Eosinofilia/etiologia , Feminino , Herpes Zoster/sangue , Humanos , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Masculino , Pessoa de Meia-Idade , Colágenos não Fibrilares/imunologia , Penfigoide Bolhoso/complicações , Índice de Gravidade de Doença , Trombofilia/sangue , Trombofilia/etiologia , Urticária/sangue
18.
Front Oncol ; 11: 679634, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34354942

RESUMO

Background: Chronic myeloid leukemia (CML) is an acquired hematopoietic stem malignant disease originating from the myeloid system. Long non-coding RNAs (lncRNAs) have been widely explored in cancer tumorigenesis. However, their roles in CML remain largely unclear. Methods: The peripheral blood mononuclear cells (PBMCs) and CML cell lines (K562, KCL22, MEG01, BV173) were collected for in vitro research. Real-time quantitative polymerase chain reaction was used to determine the mRNA expression levels. Cell viability and apoptosis were analyzed by cell counting kit 8 and flow cytometry assays. The targeting relationships were predicted using Starbase and TargetScan and ulteriorly verified by RNA pull-down and luciferase reporter assays. Western blotting assay was performed to assess the protein expressions. N6-methyladenosine (m6A) modification sites were predicted by SRAMP and confirmed by Methylated RNA immunoprecipitation (MeRIP) assay. Results: LncRNA nuclear-enriched abundant transcript 1 (NEAT1) expression levels were decreased in the CML cell lines and PBMCs of CML patients. Moreover, METTL3-mediated m6A modification induced the aberrant expression of NEAT1 in CML. Overexpression of NEAT1 inhibited cell viability and promoted the apoptosis of CML cells. Additionally, miR-766-5p was upregulated in CML PBMCs and abrogated the effects of NEAT1 on cell viability and apoptosis of the CML cells. Further, CDKN1A was proved to be the target gene of miR-766-5p and was downregulated in the CML PBMCs. Knockdown of CDKN1A reversed the effects of NEAT1. Conclusion: The current research elucidates a novel METTL3/NEAT1/miR-766-5p/CDKN1A axis which plays a critical role in the progression of CML.

19.
Clin Proteomics ; 18(1): 22, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34418970

RESUMO

BACKGROUND: Preeclampsia and gestational hypertension can cause vascular function impairment in offspring. In our previous work, we described the protein expression profiles of umbilical artery tissues from patients with preeclampsia. METHODS: To gain insights into the mechanisms of vascular dysfunction in adult rats born to preeclamptic dams, we analyzed thoracic aorta tissues by using iTRAQ isobaric tags and 2D nano LC-MS/MS. RESULTS: By using the iTRAQ method, we analyzed 1825 proteins, of which 106 showed significantly different expression in the thoracic aortic. Ingenuity pathway analysis (IPA) showed that the majority of differentially expressed proteins (DEPs) were associated with cardiovascular function. Further analysis indicated that glucose-6-phosphate dehydrogenase (G6PD), which is inhibited by miR-423-5p and activated by TP53, had the strongest effect on cardiovascular function. The expression of G6PD was upregulated in thoracic aorta tissues, as confirmed by Western blotting. The expression of two other vascular function-related proteins, cysteine- and glycine-rich protein 2 (CSRP2) and tubulin alpha-4 A (TUBA4A), was upregulated, as demonstrated by mass spectrometry (MS). CONCLUSIONS: Although the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers.

20.
Food Sci Nutr ; 9(8): 4509-4516, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34401098

RESUMO

China has the greatest rice production in the world, but the problem of heavy metal pollution in rice is becoming increasingly serious. The present study examined a microbial immobilization method to remove cadmium (Cd) in rice flour. The study demonstrated that Lactobacillus plantarum (L. plantarum) exhibited the best removal effect, but the microorganisms were difficult to separate from rice flour. Diatomaceous earth coimmobilized microbial pellets (DECIMPs) were prepared using coimmobilized L. plantarum with sodium alginate (SA, 3%), polyvinyl alcohol (PVA, 2%), and diatomaceous earth (DE, 1%). Compared with microbial fermentation, the immobilized pellets had less influence on rice quality, and Cd removal rates of sample 1 (0.459 ± 0.006 mg/kg) and 2 (0.873 ± 0.031 mg/kg) reached 90.01% ± 1.01% (0.051 ± 0.003 mg/kg) and 91.80% ± 0.54% (0.068 ± 0.034 mg/kg), which were significantly higher than free microbial fermentation. In addition, microbial was easily separated. These results show that DECIMPs fermentation is an effective means of removing Cd from rice and could be considered as a strategy for the development of Cd-free rice-based foods.

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