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1.
J Ethnobiol Ethnomed ; 17(1): 67, 2021 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-34844607

RESUMO

BACKGROUND: Marketplaces reflect not only the commerce of an area, but also its culture. In Qiandongnan Miao and Dong Autonomous Prefecture with Kaili as its capital, Guizhou Province, China, traditional medicine is thriving in both rural and urban areas. The local people rely extensively on plants for traditional medicines, and these are commonly sold in local specialized markets. The Kaili medicinal market is the biggest in the prefecture. However, ethnobotanical study on herbal plants traded in the traditional market in Kaili has not been performed. The aims of this study are: (1) to document medicinal plants traded in the Kaili traditional market and the associated traditional knowledge; and (2) to analyze the level of agreement among vendors in the purported uses of medicinal plants by using informant consensus (FIC) and the fidelity level (FL). METHODS: Market surveys were conducted in 2014-2019 to collect information about medicinal plants and associated traditional knowledge. Information including vernacular names, preparation methods, and plant uses was obtained by interviewing 116 vendors of herbal plants. Specimens of fresh and dried herbs, collected as vouchers, were identified by the authors and other botanists at the Minzu University of China, and deposited in the herbarium at Minzu University of China. The level of agreement among information provided by different vendors was assessed using the FIC, and the percentage of vendors claiming the use of a certain medicinal plant for the same indication was assessed with the FL. RESULTS: The Miao people comprise 53.4% of all informants in this study of medicinal plants. In total, 237 medicinal plant species traded in the Kaili traditional market were recorded. They belong to 219 genera and 107 families. These plants have been categorized into their purported treatments for 20 medical conditions. The inflammation category showed the highest FIC value of 0.95, showing the best agreement among market vendors claiming its usefulness to treat this condition. The FL index helped to identify 15 culturally important medicinal plant species based on the reported uses by 20 or more vendors in the market. Three medicinal plant species, Eleutherococcus gracilistylus, Sargentodoxa cuneata, and Stephania cepharantha, had an FL > 90%, being used to treat sprains/traumas, rheumatism, and heat/toxins. CONCLUSIONS: The medicinal plants sold in the Kaili market are highly diverse and have unique medicinal characteristics. The Miao people often use traditional herbal plants for disease prevention and thereby prioritize the use of medicinal plants in everyday life. The future of this medicinal marketplace, however, is uncertain since few young people (< 30 years old) are vendors or customers. Therefore, it is urgent to conserve traditional ethnomedicinal culture in local communities and pass on the associated traditional knowledge to future generations in this prefecture. And the next step should include further studies on FL > 90% plants' chemistry, pharmacology, biological activity, and toxicity for potentially developing functional foods or pharmaceutical products.

2.
Reprod Health ; 18(1): 200, 2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34627302

RESUMO

PURPOSE: To explore the hope levels and influencing factors in infertile women undergoing first-time and repeated in vitro fertilization-embryo transfer (IVF-ET) cycles. METHODS: This study was a cross-sectional and convenient sampling study conducted among patients undergoing IVF-ET from January to June 2019. Patients were divided into first-time and repeated groups by the number of IVF-ET cycles, and then a questionnaire survey was administered. The questionnaire included demographic information, Herth hope index (HHI) scale, Locke-Wallace short marital-adjustment test scale and social support rating scale. Multiple linear regression was used to analyse the influencing factors associated with hope levels. RESULTS: A total of 298 IVF-ET patients were recruited for the study, including 150 (50.3%) in the first-time cycle group and 148 (49.7%) in the repeated cycle group. The HHI score of the repeated cycle group was significantly lower than that of the first-time cycle group (34.4 ± 3.5 vs. 37.5 ± 3.7, P < 0.001). Multiple linear regression analysis indicated that repeated IVF-ET and age were independently negatively correlated with HHI, with standardized coefficient ß values of - 0.895 and - 0.223, respectively (both P < 0.001). High education level (P = 0.002), high monthly income (P = 0.020), high degree of short marital-adjustment test (P < 0.001) and social support rating (P < 0.001) were independently positively correlated with HHI. CONCLUSION: Infertile women undergoing repeated IVF-ET have low hope levels. Maintaining a good marriage adjustment and establishing a good social support and relationship network could effectively improve their hope levels.


Assuntos
Infertilidade Feminina , Estudos Transversais , Transferência Embrionária , Feminino , Fertilização In Vitro , Humanos , Infertilidade Feminina/terapia , Apoio Social
3.
World J Clin Cases ; 9(26): 7717-7728, 2021 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-34621822

RESUMO

BACKGROUND: Non-alcoholic fatty liver disease has become the most common chronic liver disease worldwide, which originates from the accumulation of triglyceride (TG) in the liver. Patients with type 2 diabetes mellitus (T2DM) are considered to have a predisposition to hepatic steatosis. However, the influencing factors for hepatic fat accumulation in T2DM patients remain unclear. AIM: To investigate the influencing factors for hepatic fat accumulation in T2DM patients. METHODS: We enrolled 329 T2DM patients admitted to the Endocrinology Department of the First Affiliated Hospital of Soochow University, who underwent MR mDIXON-Quant examination to quantify the hepatic fat fraction (HFF). According to body mass index (BMI), the patients were divided into normal weight, overweight, and obese groups. The differences in general statistics, biochemical parameters, islet function, and HFF were compared among the three groups. The associations between HFF and other parameters and the influences of various parameters on the severity of hepatic fat accumulation were analyzed. RESULTS: The HFF of T2DM patients gradually increased in the normal weight, overweight, and obese groups (P < 0.05). Spearman correlation analysis showed that in T2DM patients, HFF was negatively correlated with age and high-density lipoprotein cholesterol (P < 0.05), whereas it was positively correlated with BMI, waist-hip ratio, fasting plasma glucose, alanine aminotransferase (ALT), aspartate aminotransferase, bilirubin, glutamyl transpeptidase, lactate dehydrogenase, albumin (ALB), uric acid (UA), total cholesterol, TG, low-density lipoprotein cholesterol (LDL-C), C-reactive protein, free triiodothyronine, fasting insulin, fasting C-peptide, and homeostasis model assessment of insulin resistance (P < 0.05). Multiple linear regression analysis showed significant positive influences of BMI, ALT, LDL-C, UA, and ALB on HFF in T2DM patients (P < 0.05). Binary logistic regression analysis showed that BMI, ALT, ALB, and LDL-C were independent risk factors for moderate to severe fatty liver in T2DM patients, and obesity increased the risk of being complicated with moderate to severe fatty liver by 4.03 times (P < 0.05). CONCLUSION: The HFF of T2DM patients increases with BMI. Higher BMI, ALT, ALB, and LDL-C are independent risk factors for moderate to severe fatty liver in T2DM patients.

4.
World J Gastrointest Oncol ; 13(7): 673-683, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34322196

RESUMO

Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. Among tumor immunotherapies, neoantigen vaccines are in early human clinical trials and have demonstrated substantial efficiency. Compared with more neoantigens in melanoma, the paucity and inefficient identification of effective neoantigens in hepatocellular carcinoma (HCC) remain enormous challenges in effectively treating this malignancy. In this review, we highlight the current development of HCC neoantigens in its generation, screening, and identification. We also discuss the possibility that there are more effective neoantigens in hepatitis B virus (HBV)-related HCC than in non-HBV-related HCC. In addition, since HCC is an immunosuppressive tumor, strategies that reverse immunosuppression and enhance the immune response should be considered for the practical exploitation of HCC neoantigens. In summary, this review offers some strategies to solve existing problems in HCC neoantigen research and provide further insights for immunotherapy.

5.
Comput Methods Programs Biomed ; 206: 106140, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33979753

RESUMO

BACKGROUND AND OBJECTIVE: Early hemorrhage enlargement in hypertensive cerebral hemorrhage indicates a poor prognosis. This study aims to predict the early enlargement of cerebral hemorrhage through the intelligent texture analysis of cerebral hemorrhage after segmentation. METHODS: A total of 54 patients with hypertensive intracerebral hemorrhage were selected and divided into enlarged hematoma (enlarged group) and non-enlarged hematoma (negative group). The U-Net Neural network model and contour recognition were used to extract the brain parenchymal region, and Mazda texture analysis software was used to extract regional features. The texture features were reduced by Fisher coefficient (Fisher), classification error probability combined average correlation coefficients (POE + ACC), and mutual information (MI) to select the best feature parameters. B11 module was used to analyze the selected features. The misclassified rate of feature parameters screened by different dimensionality reduction methods was calculated. RESULTS: The neural network based on U-Net can accurately identify the lesion of cerebral hemorrhage. Among the 54 patients, 18 were in the enlarged group and 36 in the negative group. The parameters of gray level co-occurrence matrix and gray level run length matrix can be used to predict the enlargement of intracerebral hemorrhage. Among the features screened by Fisher, POE + ACC and MI, the texture features of MI showed the lowest misclassified rate, which was 0. CONCLUSION: The texture analysis based on U-Net neural network is helpful to predict the early expansion of hypertensive cerebral hemorrhage, and the parameters of gray level co-occurrence matrix and gray level run length matrix under MI dimensionality reduction have the most excellent predictive value.


Assuntos
Hemorragias Intracranianas , Redes Neurais de Computação , Encéfalo , Humanos , Hemorragias Intracranianas/diagnóstico por imagem
6.
PhytoKeys ; 177: 17-24, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33958946

RESUMO

Utricularia lihengiae, a new species from the Dulongjiang region of northwest Yunnan, China, is here described and illustrated. The new species belongs to the section Oligocista and is similar to U. bifida L. and U. scandens Benj., from which it can be easily distinguished by the dark purple stripe on the corolla. The new species also differs in its shorter inflorescence and the shape of the calyx lobes.

7.
Metab Brain Dis ; 36(5): 969-981, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33608831

RESUMO

Intrauterine growth restriction (IUGR) affects brain neural stem cell (NSC) differentiation. In the present study, we investigated whether taurine supplementation may improve NSC differentiation in IUGR fetal rats via the protein kinase A-cyclic adenosine monophosphate (cAMP) response element protein-brain derived neurotrophic factor (PKA-CREB-BDNF) signaling pathway. The IUGR fetal rat model was established with a low-protein diet. Fresh subventricular zone (SVZ) tissue from the fetuses on the 14th day of pregnancy was microdissected and dissociated into single-cell suspensions, then was cultured to form neurospheres. The neurospheres were divided into the control group, the IUGR group, the IUGR+taurine (taurine) group, the IUGR+H89 (H89) group and the IUGR+taurine+H89 (taurine+H89) group. The mRNA and protein expression levels of PKA, CREB and BDNF were measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting (WB). Tuj-1-positive neurons and GFAP-positive glial cells were detected by immunofluorescence. The total number of proliferating NSCs and the percentage of Tuj-1-positive neurons in the IUGR group were lower than those in the control group, but the percentage of GFAP-positive cells was higher in the IUGR group than in the control group. Taurine supplementation increased the total number of neural cells and the percentage of Tuj-1-positive neurons, and reduced the percentage of GFAP-positive cells among differentiated NSCs after IUGR. H89 reduced the total number and percentage of Tuj-1-positive neurons and increased the percentage of GFAP-positive cells. The mRNA and protein levels of PKA, CREB, and BDNF were lower in the IUGR group. The mRNA and protein expression levels of these factors were increased by taurine supplementation but reduced by the addition of H89. Taurine supplementation increased the ratio of neurons to glial cells and prevented gliosis in the differentiation of NSCs in IUGR fetal rats by activating the PKA-CREB-BDNF signaling pathway.

8.
Zhongguo Zhong Yao Za Zhi ; 45(21): 5150-5159, 2020 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-33350230

RESUMO

The study is aimed to reveal the fluctuation of the inorganic elements in the rhizosphere soil of Ligusticum chuanxiong during their whole growth period, and explore the relationship between that fluctuation and the formation of radial striations character in the rhizomes. During the cultivation period of L. chuanxiong, the rhizosphere soil samples were taken regularly, and the content of 26 inorganic elements were determined by X-ray fluorescence spectrometry(XRF). Then the difference between the radial striations and un-radial striations rhizomes were analyzed for their fluctuation of the inorganic elements. The results showed that there were different "key period" and "key elements" in the rhizosphere elements content of L. chuanxiong rhizome with radial and un-radial striations, and different element coordination and antagonistic relationship. The key fluctuation period of rhizosphere elements in un-radial striations group were in 0-60 and 60-150 days, of which 22 elements such as Na, Mg, Al were the key elements in 0-60 days, and 5 elements such as Sr, Hf, Pb, Co, Ce were the key elements in 60-150 days. The key fluctuation period of rhizosphere elements in radial striations group were in 0-60 and 210-270 days, of which four elements such as Na, Co, Ce, As are the key change elements in 0-60 days, and 18 elements such as Mg, Al, Si are the key change elements in 210-270 days. At the same time, the study showed that the fluctuation of inorganic elements in rhizosphere soil coincided with the growth and development process of L. chuanxiong and the key period of the formation of "radial striations rhizome". The key stage which the rapid growth of lateral buds of rhizome affected the formation of radial striations is 60-150 days after planting, while the increase of Sr and Co elements is likely to be an important reason for the expansion of lateral buds of rhizome and the failure to form typical "radial striations rhizome" in un-radial striations group.


Assuntos
Medicamentos de Ervas Chinesas , Ligusticum , Rizoma , Rizosfera , Solo
9.
Antibiotics (Basel) ; 9(12)2020 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-33327385

RESUMO

As the causative agent of Glässer's disease, Glaesserella (Haemophilus) parasuis has led to serious economic losses to the swine industry worldwide. Due to the low cross-protection of vaccines and increasing antimicrobial resistance of G. parasuis, it is important to develop alternative approaches to prevent G. parasuis infection. Defensins are host defense peptides that have been suggested to be promising substitutes for antibiotics in animal production, while porcine ß-defensin 2 (PBD-2) is a potent antimicrobial peptide discovered in pigs. Our previous study generated transgenic (TG) pigs overexpressing PBD-2, which displayed enhanced resistance to Actinobacillus pleuropneumoniae. In this study, the antibacterial activities of PBD-2 against G. parasuis are determined in vitro and in the TG pig model. The concentration-dependent bactericidal activity of synthetic PBD-2 against G. parasuis was measured by bacterial counting. Moreover, after being infected with G. parasuis via a cohabitation challenge model, TG pigs overexpressing PBD-2 displayed significantly milder clinical signs and less severe gross pathological changes than their wild-type (WT) littermates. The TG pigs also exhibited alleviated lung and brain lesions, while bacterial loads in the lung and brain tissues of the TG pigs were significantly lower than those of the WT pigs. Additionally, lung and brain homogenates from TG pigs possessed enhanced antibacterial activity against G. parasuis when compared with those from the WT pigs. Altogether, these proved that overexpression of PBD-2 could also endow pigs with increased resilience to G. parasuis infection, which further confirmed the potential of using the PBD-2 coding gene to develop disease-resistant pigs and provided a novel strategy to combat G. parasuis as well.

10.
J Ethnobiol Ethnomed ; 16(1): 61, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33054863

RESUMO

BACKGROUND: The Dulong (Drung) people have used plant materials in traditional beekeeping for many decades. However, there are few studies on the plants used in traditional beekeeping. Furthermore, traditional ecological knowledge (TEK) associated with beekeeping is still poorly understood. TEK and plants associated with beekeeping play an important role in the conservation of native bees and the development of beekeeping. It is therefore very urgent to investigate, record, and study the plants and TEK of Dulong beekeeping. METHODS: Fieldwork was conducted in the Dulong community of Gongshan County, Yunnan Province, China. Six Dulong villages were investigated. Ethnobotanical methods such as free listing, semi-structured interviews, participatory observation, and key informant interviews were used to collect data. A total of 42 Dulong respondents provided information about plants used in traditional beekeeping. TEK related to traditional beekeeping plants was documented. Citation frequency, abundance, and preference ranking of log beehive plant species were used to identify plant resources that are "easier to obtain" and "more preferred." RESULTS: There are two general methods of traditional Dulong beekeeping: living tree beekeeping and log beehive beekeeping. The investigation revealed that 38 species (in 19 families), including 30 tree species, 5 bamboo species, 2 herbaceous species, and 1 liana species, are used in traditional Dulong beekeeping. Different plant parts are used for different purposes. Twenty-seven tree species are used to make log beehives. Species from the family Pinaceae and Fagaceae are the most frequently represented. Seven of the most commonly reported species used to build log beehives were scored by ten beekeepers. Based on this scoring, the beekeepers' most preferred species for making log beehives are Alnus nepalensis, Pinus yunnanensis, and Juglans regia. CONCLUSION: The Dulong people have used various plants for traditional beekeeping and have accumulated rich TEK associated with apiculture. Future research will include a nutritive components analysis of honey from traditional Dulong beekeeping and an ethnobotanical investigation of melliferous species used in traditional Dulong apicultural systems. The application of plants and TEK associated with beekeeping is important for improving livelihoods in local communities, conserving biocultural diversity, and protecting the eco-environment of the Dulongjiang area.


Assuntos
Criação de Abelhas , Grupos Étnicos , Etnobotânica , Conhecimento , Adulto , Idoso , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
12.
Cancer Nurs ; 2020 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-32694281

RESUMO

BACKGROUND: Cancer-related fatigue is a complex, multidimensional, subjective experience that affects patients physically, emotionally, and mentally. The interindividual variability in symptoms of cancer-related fatigue merits further exploration. OBJECTIVE: Our objective was to identify distinct profiles of cancer-related fatigue experienced by breast cancer patients undergoing chemotherapy and to evaluate how subgroups vary demographically in clinical characteristics and in modifiable factors such as physical activity, sleep quality, and exercise self-efficacy. METHODS: Fatigue was assessed with the Chinese Cancer-Related Fatigue Scale, and a latent class analysis was performed to identify subgroups of patients with distinct fatigue profiles. RESULTS: A total of 427 breast cancer patients were included in the data analyses. Five different fatigue profiles were identified: all low-risk fatigue, all high-risk fatigue, high-risk physical fatigue, high-risk emotional fatigue, and high-risk mental fatigue. Patients in different subgroups were characterized by different risk factors. For example, patients in the high-risk emotional fatigue group had less education, lower monthly household incomes, lower exercise self-efficacy scores, less sedentary behavior, poorer sleep, and poorer quality-of-life outcomes compared with those in the all low-risk fatigue group. CONCLUSION: These findings reveal that breast cancer patients undergoing chemotherapy show significant heterogeneity in their experience of cancer-related fatigue. IMPLICATIONS FOR PRACTICE: Characteristics associated with different fatigue profiles, in particular the high-risk profiles, can be used by clinicians to target patients at greater risk of poorer symptom and quality-of-life outcomes to provide interventions tailored to their different needs.

13.
Artif Cells Nanomed Biotechnol ; 48(1): 933-938, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32496828

RESUMO

Background: miR-4293 rs12220909 polymorphism was reported associated with tumorigenesis, but the results are controversial. Thus, we planned to verify and obtain precise results.Methods: Databases were searched and reviewed up to November, 2019. Case-control studies which concern about the association between cancer risks and miR-4293 polymorphisms were all enrolled. The odds ratios (ORs) and 95% confidence intervals (CIs) calculated by the Z test were used to assess the underlying links. We also prospected how the miR-4293 impacts biological process through its target genes.Result: Finally, there are seven independent studies meet the enrolled criteria, along with 5147 cases and 6108 healthy controls. We revealed that there is a significant decrease effect of miR-4293 rs12220909 to cancer risks in heterozygote genetic model (BA vs. AA: OR = 0.857, p = .032), the similar results were also uncovered in PB control group, lung cancer and the studies conform to HWE. Results from GO items and KEGG pathway analysis illustrated that myeloid cell development, transcription factor complex, RNA polymerase II regulatory region DNA binding were regulated by miR-4293.Conclusion: In summary, our meta-analysis chase down heterozygote rs12220909 polymorphism of miR-4293 is a protective factor to cancer initiation, especially for lung cancer.


Assuntos
Predisposição Genética para Doença/genética , MicroRNAs/genética , Neoplasias/genética , Polimorfismo Genético , Estudos de Casos e Controles , Humanos , Razão de Chances
14.
Oncol Rep ; 43(6): 1863-1874, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32236588

RESUMO

Lung cancer has one of the highest mortalities of any cancer worldwide. Triptolide (TP) is a promising tumor suppressor extracted from the Chinese herb Tripterygium wilfordii. Our previous proteomics analysis revealed that TP significantly interfered with the ribosome biogenesis pathway; however, the underlying molecular mechanism remains poorly understood. The aim of the present study was to determine the molecular mechanism of TP's anticancer effect by investigating the association between ribosomal stress and p53 activation. It was found that TP induces nucleolar disintegration together with RNA polymerase I (Pol I) and upstream binding factor (UBF) translocation. TP interrupted ribosomal (r)RNA synthesis through inhibition of RNA Pol I and UBF transcriptional activation. TP treatment increased the binding of ribosomal protein L23 (RPL23) to mouse double minute 2 protein (MDM2), resulting in p53 being released from MDM2 and stabilized. Activation of p53 induced apoptosis and cell cycle arrest by enhancing the activation of p53 upregulated modulator of apoptosis, caspase 9 and caspase 3, and suppressing BCL2. In vivo experiments showed that TP significantly reduced xenograft tumor size and increased mouse body weight. Immunohistochemical assays confirmed that TP significantly increased the p53 level and induced nucleolus disintegration, during which nucleolin distribution moved from the nucleolus to the nucleoplasm, and RPL23 clustered at the edge of the cell membrane. Therefore, it was proposed that TP induces ribosomal stress, which leads to nucleolus disintegration, and inhibition of rRNA transcription and synthesis, resulting in increased binding of RPL23 with MDM2. Consequently, p53 is activated, which induces apoptosis and cell cycle arrest.


Assuntos
Antineoplásicos Alquilantes/administração & dosagem , Diterpenos/administração & dosagem , Neoplasias Pulmonares/tratamento farmacológico , Fenantrenos/administração & dosagem , RNA Ribossômico/metabolismo , Transdução de Sinais/efeitos dos fármacos , Células A549 , Animais , Antineoplásicos Alquilantes/farmacologia , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Diterpenos/farmacologia , Compostos de Epóxi/administração & dosagem , Compostos de Epóxi/farmacologia , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/metabolismo , Masculino , Camundongos , Fenantrenos/farmacologia , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteínas Ribossômicas/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
15.
Food Chem ; 321: 126707, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32244134

RESUMO

Mealiness is one of the most important textural failure of apple fruit and four patterns of mealiness involving five apple cultivars were identified as the rapid, moderate, slow and none, requiring 3, 7, 14, 49 days at 25 °C, respectively. In comparison with the non-mealy 'Fuji' apple, parenchyma cells of mealy apples became detached and remained intact. Highly methyl-esterified homogalacturonan was strongly immunolabeled in the cell wall of slow and non-mealy apples. The mobility of water was enhanced in the cell wall during mealiness. Principal components analysis of FTIR spectra discriminated the cell wall materials (CWM) based on the mealiness progress. Heavy loss of CWM and its water-insoluble fractions but limited increase of water-soluble fractions, and the increase of crystalline micelles of CWM were closely associated with the mealiness progress. Overall, the occurrence of mealiness might attribute to structural, physical and biochemical modifications of CWM during tissue senescence.


Assuntos
Parede Celular/química , Malus/química , Pectinas/química , Frutas/química , Refeições
16.
Virology ; 545: 1-9, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32174453

RESUMO

The emergence of resistant mutants to the wildly used neuraminidase inhibitors (NAIs) makes the development of novel drugs necessary. Favipiravir (T-705) is one of the RNA-dependent RNA polymerase (RdRp) inhibitors developed in recent years. To examine the efficacy of T-705 against influenza B virus infections in vivo, C57BL/6 mice infected with wild-type or oseltamivir-resistant influenza B/Memphis/20/96 viruses were treated with T-705. Starting 2 h post inoculation (hpi), T-705 was orally administered to mice BID at dosages of 50, 150, or 300 mg/kg/day for 5 days. Oseltamivir was used as control. Here, we showed that T-705 protected mice from lethal infection in a dose-dependent manner. T-705 administration also significantly reduced viral loads and suppressed pulmonary pathology. In addition, phenotypic assays demonstrated that no T-705-resistant viruses emerged after T-705 treatment. In conclusion, T-705 can be effective to protect mice from lethal infection with both wild-type and oseltamivir-resistant influenza B viruses.


Assuntos
Amidas/administração & dosagem , Antivirais/administração & dosagem , Farmacorresistência Viral , Vírus da Influenza B/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Oseltamivir/administração & dosagem , Pirazinas/administração & dosagem , Animais , Feminino , Humanos , Vírus da Influenza B/genética , Vírus da Influenza B/fisiologia , Influenza Humana/virologia , Camundongos , Camundongos Endogâmicos C57BL
17.
Artigo em Inglês | MEDLINE | ID: mdl-32167906

RESUMO

The advent of single-cell RNA sequencing (scRNA-seq) techniques opens up new opportunities for studying the cell-specific changes in the transcriptomic data. An important research problem related with scRNA-seq data analysis is to identify cell subpopulations with distinct functions. However, the expression profiles of individual cells are usually measured over tens of thousands of genes, and it remains a difficult problem to effectively cluster the cells based on the high-dimensional profiles. An additional challenge of performing the analysis is that, the scRNA-seq data are often noisy and sometimes extremely sparse due to technical limitations and sampling deficiencies. In this paper, we propose a biclustering-based framework called DivBiclust that effectively identifies the cell subpopulations based on the high-dimensional noisy scRNA-seq data. Compared with nine state-of-the-art methods, DivBiclust excels in identifying cell subpopulations with high accuracy as evidenced by our experiments on ten real scRNA-seq datasets with different size and diverse dropout rates. The supplemental materials of DivBiclust, including the source codes, data, and a supplementary document, are available at https://www.github.com/Qiong-Fang/DivBiclust.

18.
Cancer Manag Res ; 12: 905-912, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32104072

RESUMO

Purpose: We aim to test whether resilience mediates the association of fear of progression (FoP) with quality of life (QoL) among ovarian cancer patients in China. Methods: We collected 230 questionnaires from the First Affiliated Hospital of China Medical University in Liaoning Province, and 209 completed the questionnaire survey. The survey instrument consisted of four questionnaires: a sociodemographic and clinical characteristics questionnaire, the Functional Assessment of Cancer Therapy general instrument, the Fear of Progression Questionnaire-Short Form and the Connor-Davidson Resilience Scale. Hierarchical regression analysis was used to examine the relationship among FoP, resilience, and QoL, including physical well-being, social well-being, emotional well-being, and functional well-being. We used asymptotic and resampling strategies to examine the indirect effect of resilience. Results: FoP was negatively associated with resilience (r=-0.543, P<0.01) and QoL (physical well-being: r=-0.537, P<0.01; social well-being: r=-0.426, P<0.01; emotional well-being: r=-0.487, P<0.01; functional well-being: r=-0.529, P<0.01). Resilience was positively related with QoL (physical well-being: r=0.449, P<0.01; social well-being: r=0.548, P<0.01; emotional well-being: r=0.430, P<0.01; functional well-being: r=0.655, P<0.01). Resilience partly mediated the association between FoP and physical well-being (a×b=-0.05, BCa 95% CI: -0.09, -0.02), social well-being (a×b=-0.21, BCa 95% CI: -0.29, -0.14), emotional well-being (a×b=-0.05, BCa 95% CI: -0.08, -0.02), and functional well-being (a×b=-0.24, BCa 95% CI: -0.32, -0.17). The proportion of the mediating effect accounted for by resilience were 22.57%, 57.22%, 26.02%, 53.42% for physical well-being, social well-being, emotional well-being and functional well-being, respectively. Conclusion: The study showed that resilience could mediate the association between fear of progression and quality of life. It suggests that resilience might provide a potential target for intervention in quality of life with ovarian cancer.

19.
Psychol Res Behav Manag ; 13: 97-104, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32095086

RESUMO

Objective: The objective of this study was to explore the association between perceived organizational support (POS) and depressive symptoms, and to further explore whether self-efficacy can act as a moderator between POS and depressive symptoms among Chinese petroleum workers. Methods: There was a cross-sectional study conducted at a petrochemical enterprise in Liaoning Province, China, from July to August 2018. A series of questionnaires were accomplished by 1836 petroleum workers, including the Center for Epidemiologic Studies Depression Scale (CES-D), the Survey of Perceived Organizational Support (SPOS), and the General Self-Efficacy Scale (GSES). Hierarchical regression analysis was used to examine the relationship of SPOS score, GSES score, and SPOS score×GSES score interaction with CES-D score. A simple slope analysis will be carried out if the interaction has statistical significance. Results: Hierarchical regression analysis showed that SPOS score (ß=-0.538, P<0.01) and GSES score (ß=-0.313, P<0.01) played a main influence on CES-D score. The SPOS score×GSES score interaction term significantly explained an extra 9.7% of the variance (F=253.932, adjusted R 2=0.582, ΔR 2=0.097, P<0.01). The interaction term was positively correlated with CES-D score (ß=0.334, P<0.01). The relationship between SPOS score and CES-D score gradually decreased in the low (1 SD below the mean, ß=-0.589, P<0.01), mean (ß=-0.338, P<0.01), and high (1 SD above the mean, ß=-0.087, P<0.01) groups of GSES score. Conclusion: This study showed that POS and self-efficacy played a main influence on depressive symptoms, and the interaction term was positively correlated with depressive symptoms. Self-efficacy could attenuate the association between POS and depressive symptoms. It suggests that appropriate POS and self-efficacy enhancement measures ought to be supplied to relieve depressive symptoms.

20.
Microbiol Res ; 230: 126343, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31539852

RESUMO

Identifying the direct target genes of response regulators (RRs) of a bacterial two-component system (TCS) is critical to understand the roles of TCS in bacterial environmental adaption and pathogenesis. Actinobacillus pleuropneumoniae is an important respiratory bacterial pathogen that causes considerable economic losses to swine industry worldwide. The targets of A. pleuropneumoniae NarP (nitrate/nitrite RR), which is the cognate RR of the nitrate/nitrite sensor histidine kinase NarQ, are still unknown. In the present study, a DNA-affinity-purified sequencing (DAP-Seq) approach was established. The upstream regions of a total of 131 candidate genes from the genome of A. pleuropneumoniae were co-purified with the activated NarP protein. Electrophoretic mobility shift assay (EMSA) results confirmed the interactions of NarP with the promoter regions of five selected target genes, including dmsA, pgaA, ftpA, cstA and ushA. The EMSA-confirmed target genes were significantly up-regulated in the narP-deleted mutant in the presence of additional nitrate, whilst the transcriptional changes were restored in the complemented strain. The NarP binding motif in the upstream regions of the target genes dmsA and ftpA were further identified and confirmed by EMSA using the truncated binding motif. The NarP binding sites were present in a total of 25.2% of the DNA fragments captured by DAP-Seq. These results demonstrated that the established DAP-Seq method is effective for exploring the direct targets of RRs of bacterial TCSs and that the A. pleuropneumoniae NarP could be a repressor in response to nitrate.


Assuntos
Actinobacillus pleuropneumoniae/genética , Proteínas de Bactérias/metabolismo , Proteínas de Ligação a DNA/metabolismo , Análise de Sequência de DNA/métodos , Actinobacillus pleuropneumoniae/metabolismo , Proteínas de Bactérias/genética , Proteínas de Ligação a DNA/genética , Ensaio de Desvio de Mobilidade Eletroforética , Regulação Bacteriana da Expressão Gênica , Nitratos/metabolismo , Nitritos/metabolismo , Regiões Promotoras Genéticas , Ligação Proteica , Proteínas Quinases/genética , Proteínas Quinases/metabolismo
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