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1.
Mol Cancer ; 19(1): 9, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31941509

RESUMO

BACKGROUND: MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown. METHODS: RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. RESULTS: We observed that lncRNA JPX was upregulated in lung cancer metastatic tissues and was closely correlated with tumor size and an advanced stage. Functionally, JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in vivo. Additionally, JPX upregulated Twist1 by competitively sponging miR-33a-5p and subsequently induced EMT and lung cancer cell invasion. Interestingly, JPX and Twist1 were coordinately upregulated in lung cancer tissues and cells. Mechanically, the JPX/miR-33a-5p/Twist1 axis participated in EMT progression by activating Wnt/ß-catenin signaling. CONCLUSIONS: These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy.

2.
Sci Total Environ ; 703: 135475, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-31767296

RESUMO

Ectomycorrhizal (ECM) fungi are closely related to vegetation compositions, edaphic properties, and site-specific processes. However, the coevolutionary mechanisms underlying the spatial distributions in floristic and ECM fungal composition in the context of biotic adaptations and abiotic variances remain unclear. We combine a total of 25 ECM fungus-associated environmental variables to impose three types of composite scores and then quantify the environmental gradients of geographical site, soil chemical property and vegetation functional trait across 122 grids of 20 m × 20 m in a 25-hm2 forest plot. Significant dissimilarities in vegetational and ECM fungal abundance and composition existed along the above environmental gradients. Specifically, a contrasting floristic distribution (e.g., Betula platyphylla vs. Tilia mandshurica) existed between the northeastern and southwestern areas and was closely related to the nutrient and moisture gradients (with high levels in the west and low levels in the east). Furthermore, the ECM fungal communities were more abundant in the nutrient-poor and low-moisture environments than in the nutrient-rich and high-moisture environments, and the mixed-forest in the middle-gradient sites between the northeastern and southwestern areas harbored the highest ECM fungal diversity. These findings suggest that predictable within-site vegetation succession is closely related to ECM-associated determinants and the natural spatial heterogeneity of edaphic properties at a local scale.

3.
Leukemia ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31776466

RESUMO

Recurrent oncogenic mutations of MyD88 have been identified in a variety of lymphoid malignancies. Gain-of-function mutations of MyD88 constitutively activate downstream NF-κB signaling pathways, resulting in increased cellular proliferation and survival. However, whether MyD88 activity can be aberrantly regulated in MyD88-wild-type lymphoid malignancies remains poorly understood. SPOP is an adaptor protein of CUL3-based E3 ubiquitin ligase complex and frequently mutated genes in prostate and endometrial cancers. In this study, we reveal that SPOP binds to and induces the nondegradative ubiquitination of MyD88 by recognizing an atypical SPOP-binding motif in MyD88. This modification blocks Myddosome assembly and downstream NF-κB activation. SPOP is mutated in a subset of lymphoid malignancies, including diffuse large B-cell lymphoma (DLBCL). Lymphoid malignancies-associated SPOP mutants exhibited impaired binding to MyD88 and suppression of NF-κB activation. The DLBCL-associated, SPOP-binding defective mutants of MyD88 escaped from SPOP-mediated ubiquitination, and their effect on NF-κB activation is stronger than that of wild-type MyD88. Moreover, SPOP suppresses DLBCL cell growth in vitro and tumor xenograft in vivo by inhibiting the MyD88/NF-κB signaling. Therefore, SPOP acts as a tumor suppressor in DLBCL. Mutations in the SPOP-MyD88 binding interface may disrupt the SPOP-MyD88 regulatory axis and promote aberrant MyD88/NF-κB activation and cell growth in DLCBL.

4.
Org Lett ; 21(23): 9598-9602, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31763857

RESUMO

An efficient rhodium-catalyzed redox-neutral annulations of N-phenoxyacetamides and ynones via successive double C-H bond activations has been developed. A series of novel and complex indenols bearing a benzofuran unit were generated with moderate to excellent regioselecetivities under mild conditions. Adding N-ethylcyclohexanamine (CyNHEt) could restrict the formation of the mono C-H bond activation byproduct, which is not the intermediate of the reaction demonstrated via the mechanistic investigations.

5.
Ecol Indic ; 1072019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31478008

RESUMO

Understanding the effects of plant species diversity and trait composition on aboveground biomass is a central focus of ecology and has important implications for biodiversity conservation. However, the simultaneous direct and indirect effects of soil nutrients, species asynchrony, functional trait diversity, and trait composition for explaining the community temporal stability of aboveground biomass remain underrepresented in natural forests. Here, we hypothesized that species asynchrony relative to soil nutrients, functional trait diversity, and trait composition plays a central role in stabilizing the community temporal stability of natural forests. We tested this hypothesis using a structural equation model based on 10-year continuous monitoring data (i.e., three-time repeated forest inventories) in both second-growth and old-growth temperate forests in northeast China. Our results showed that the community temporal stability of aboveground biomass was driven by a strong direct positive effect of species asynchrony in both second-growth and old-growth temperate forests, whereas functional trait diversity and composition (i.e. community-weighted mean of leaf nitrogen content) were of additional importance in an old-growth forest only. Functional trait diversity decreased community-weighted mean of leaf nitrogen content in an old-growth forest, whereas this relationship was non-significant in a second-growth forest. Soil nutrients had non-significant effects on the community temporal stability of both second-growth and old-growth forests. Species asynchrony was the direct determinant of the community temporal stability of aboveground biomass in temperate forests. The direct effect of species asynchrony increased with forest succession, implying that temporal niche differentiation and facilitation increase over time. This study suggests that managing forests with mixtures of both early and late successional species or shade intolerant and tolerant species, not only species diversity, is important for maintaining forest stability in a changing environment. We argue that the species asynchrony effect is crucial to understand the underlying ecological mechanisms for a diversity-biomass relationship in natural forests.

6.
Ultrason Sonochem ; 57: 98-107, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31208623

RESUMO

In this study, we used flotation tests, Fourier transform infrared spectroscopy (FTIR), zeta potential, X-ray photoelectron spectroscopy (XPS), and microcalorimetry measurements to investigate the flotation and possible adsorption mechanisms of the ilmenite surface before and after ultrasonic pre-treatment. Flotation results show that under optimum conditions, the promotion effect of sonication on ilmenite is remarkable. The maximum recovery is 89.54% for ultrasonicated ilmenite at a pH of 4-5. For pH of 8-9, recovery increased again to 66.34%. Microcalorimetry indicates that the adsorption-driven heat release (-Qads) is higher for ultrasonicated ilmenite than for raw one. After pre-treatment, the iso-electric point (IEP) changed from pH 6.2 to pH 4.2. FTIR spectra and zeta potential measurements indicated that metal ions as active sites on the ilmenite surface are probably changed by the ultrasonic treatment. XPS analysis shows that ultrasonic treatment can promotes the oxidation of Fe2+ to Fe3+ and improves the solubilization of Ca2+ and Mg2+ in the pH range of 4-5. Under weakly alkaline condition, ultrasound also can make Ca2+ and Mg2+ re-absorb onto the ilmenite surface as main active sites.

7.
Clin Chim Acta ; 495: 331-337, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31054913

RESUMO

Despite many advances in diagnostics and multimodal treatment (surgery, radiotherapy, chemotherapy), cancer still remains one of the most important public health challenges worldwide because of the associated morbidity and mortality. Liquid biopsy has been developed to detect cancer at an early stage based on minimally invasive and serial body fluid tests with the advantage of following tumor evolution in real time. Circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), circulating cell-free noncoding RNAs (cfRNAs) and circulating exosomes represent the major components of liquid biopsy analysis. Liquid biopsy already has been implemented in cancer management, and most studies thus far are mainly focused on CTCs and ctDNA. In fact, the circulating long noncoding RNAs (lncRNAs) in exosomes have been discovered and confirmed to be closely related to tumorigenesis, metastasis and therapy. Thus this review is mainly focused on the clinical potential of circulating exosomal lncRNAs as a source of liquid biopsy biomarkers in cancer diagnosis, prognosis, and response to treatment, offering novel insights into the precision medicine of oncology.


Assuntos
Biomarcadores Tumorais/sangue , Biópsia Líquida/métodos , Neoplasias/diagnóstico , RNA Longo não Codificante/sangue , DNA Tumoral Circulante/sangue , Exossomos/genética , Humanos , Células Neoplásicas Circulantes/patologia , Prognóstico
8.
Medicine (Baltimore) ; 98(19): e15391, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31083167

RESUMO

RATIONALE: Pleuroperitoneal communication (PPC) has been reported to complicate continuous ambulatory peritoneal dialysis (CAPD). However, cases of patients in whom the results of the methylene blue dye test and peritoneopleural scintigraphy were negative and treatment was thoracoscopic surgery have been rarely reported. PATIENT CONCERNS: A 58-year-old man with end-stage chronic renal failure who underwent CAPD presented with massive right-sided hydrothorax. The pleural fluid glucose level was high. Results of both the methylene blue dye test and peritoneopleural scintigraphy were negative. DIAGNOSIS: The presence of end-stage chronic renal failure and diaphragm defects amenable to repair, which were identified during thoracoscopic surgery, indicated a definite diagnosis of PPC complicating CAPD. INTERVENTIONS AND OUTCOMES: CAPD was performed twice after the defects were repaired during thoracoscopic surgery. There was no evidence that the repaired sites were leaking again, and the patient did not complain of any discomfort during the second CAPD. LESSON: Although special methods such as the methylene blue dye test and peritoneopleural scintigraphy may not be useful in some cases, thoracoscopic surgery is still effective and reliable in diagnosing and repairing diaphragmatic defects.


Assuntos
Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Doenças Peritoneais/cirurgia , Doenças Pleurais/cirurgia , Toracoscopia , Diagnóstico Diferencial , Humanos , Falência Renal Crônica/terapia , Masculino , Pessoa de Meia-Idade , Doenças Peritoneais/diagnóstico , Doenças Peritoneais/etiologia , Doenças Pleurais/diagnóstico , Doenças Pleurais/etiologia , Toracoscopia/métodos
9.
BMC Cancer ; 19(1): 500, 2019 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-31132995

RESUMO

BACKGROUND: To investigate the long-term efficacy of the minimally invasive Ivor Lewis esophagectomy (MIILE) in esophageal squamous cell carcinoma (ESCC) patients, a retrospective comparison of the quality of life (QOL) and survival between patients who underwent MIILE and left transthoracic esophagectomy (Sweet approach) was conducted. METHODS: A detailed database search identified 614 patients who underwent MIILE and 243 patients who underwent Sweet esophagectomy between January 2011 and December 2017. After propensity score matching, 216 paired cases were selected for statistical analysis. Survival was evaluated with Kaplan-Meier curves or Cox models. RESULTS: MIILE was associated with a longer duration, less blood loss and more lymph node dissected than Sweet esophagectomy. MIILE patients suffered from less pain, less frequently developed pneumonia, and had fewer postoperative complications. Additionally, MIILE patients began oral intake earlier and had a shorter postoperative hospital stay, and enhanced recovery of QOL. There was no significant difference between the approaches regarding the recurrence pattern, 2-year and 5-year overall survival (OS) or disease-free survival (DFS), except that patients with tumor-node-metastasis (TNM) stage I in the MIILE group demonstrated superior OS and DFS. Pathological TNM stage and postoperative complications were determined to be independent prognostic factors based on the multivariate analysis. CONCLUSION: MIILE is a safe and feasible approach for treating ESCC patients. MIILE approach may provide more postoperative advantages, enhanced QOL improvement, and more favorable long-term survival in early stage patients than the Sweet procedure.


Assuntos
Neoplasias Esofágicas/cirurgia , Carcinoma de Células Escamosas do Esôfago/cirurgia , Esofagectomia/efeitos adversos , Esofagectomia/métodos , Procedimentos Cirúrgicos Minimamente Invasivos/efeitos adversos , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Pontuação de Propensão , Idoso , Intervalo Livre de Doença , Ingestão de Alimentos , Feminino , Seguimentos , Humanos , Estimativa de Kaplan-Meier , Laparoscopia/métodos , Tempo de Internação , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/etiologia , Modelos de Riscos Proporcionais , Qualidade de Vida , Estudos Retrospectivos , Resultado do Tratamento
10.
BMC Cancer ; 19(1): 8, 2019 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-30611231

RESUMO

BACKGROUND: In this retrospective study, we aimed to demonstrated that three-port single-intercostal (SIC) thoracoscopic lobectomy is an effective choice for lung cancer by comparing the perioperative outcomes of patients with non-small-cell lung cancer treated with three-port SIC and conventional multiple-intercostal (MIC) thoracoscopic lobectomy. METHODS: From January 2013 to January 2018, 642 non-small-cell lung cancer patients underwent thoracoscopic lobectomy via a three-port SIC or MIC technique. Propensity-matched analysis incorporating preoperative clinical variables was used to compare the perioperative outcomes between the two groups. RESULTS: The first 20 patients were excluded to account for the learning curve effect in the SIC group. Propensity matching yielded 186 patients in each group. A small percentage of patients had major morbidity, including 4.8% in the SIC group and 6.5% in the MIC group; there was no significant difference between the two groups. Although the total number of lymph nodes harvested (25.3 vs. 23.8, p = 0.160) and stations removed (6.5 vs. 6.7, p = 0.368) were similar between the two groups, more subcarinal lymph nodes were removed (6.9 vs. 5.2, p < 0.001) in the SIC group than in the MIC group. Furthermore, other perioperative outcomes in the SIC group were not fewer than those in the MIC group. CONCLUSIONS: Both techniques are acceptable for the treatment of non-small-cell lung cancer. Three-port SIC VATS lobectomy can provide an alternative procedure in thoracoscopic surgery.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/cirurgia , Neoplasias Pulmonares/cirurgia , Pulmão/cirurgia , Pneumonectomia , Adulto , Idoso , Carcinoma Pulmonar de Células não Pequenas/complicações , Carcinoma Pulmonar de Células não Pequenas/patologia , Feminino , Humanos , Tempo de Internação , Pulmão/patologia , Neoplasias Pulmonares/complicações , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/patologia , Cirurgia Torácica Vídeoassistida/efeitos adversos , Cirurgia Torácica Vídeoassistida/métodos , Resultado do Tratamento
11.
Curr Gene Ther ; 19(2): 125-133, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30411680

RESUMO

Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) that structurally form closed loops without 5'-end cap and 3'-end poly(A) tail unlike linear RNAs. CircRNAs are widely present in eukaryotic cells with the capabilities of structural stability, high abundance and cell- /tissue-specific expression. A growing body of researches suggest that the dysregulated circRNAs are intimately relevant to the occurrence and development of cancer. In this review, we mainly discuss the differentially expressed circRNAs in cancer tissues, plasma and exosomes, which makes it possible for clinicians to use certain circRNAs as novel biomarkers for cancer diagnosis and prognosis. In particular, we primarily focus on circRNAs as potential therapeutic targets, which will provide promising applications in cancer gene therapy.

12.
Sci Rep ; 8(1): 16699, 2018 11 12.
Artigo em Inglês | MEDLINE | ID: mdl-30420640

RESUMO

MicroRNAs (MiRNAs) have been found to be dysregulated in lung cancer tissues compared to their matched paracancerous tissues. However, the roles of miRNAs in peripheral blood as potential biomarkers for early diagnosis of lung cancer remain poorly understood. Here we found that miR-33a-5p and miR-128-3p were down-regulated in lung cancer tissues and cell lines. The expression levels of miR-33a-5p and miR-128-3p in lung cancer tissues were significantly correlated to TNM stages. MiR-128-3p in lung cancer tissues was also remarkably related to smoking and tumor size. The relative expression levels of miR-33a-5p and miR-128-3p were positively correlated in lung cancer tissues. Notably, miR-33a-5p and miR-128-3p in whole blood of lung cancer patients or early-stage lung cancer patients (TNM stage I-II) were lowly expressed as compared with that in healthy controls. The receiver operating characteristic curve (ROC) analyses revealed higher area under the ROC curve (AUC) values and higher sensitivity/specificity of miR-33a-5p and miR-128-3p alone and in combination were superior to that of traditional tumor markers (CYFR21-1, NSE and CA72-4). Importantly, both miR-33a-5p and miR-128-3p in whole blood were highly stable even under different harsh conditions. The results demonstrate that tumor suppressor miR-33a-5p/miR-128-3p in whole blood can serve as novel biomarkers for the early detection of lung cancer.


Assuntos
Biomarcadores Tumorais/genética , Detecção Precoce de Câncer/métodos , Neoplasias Pulmonares/genética , MicroRNAs/genética , Linhagem Celular , Linhagem Celular Tumoral , Humanos , Curva ROC , Reação em Cadeia da Polimerase em Tempo Real
13.
PLoS One ; 13(11): e0208200, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30496254

RESUMO

BACKGROUND: China adopted family doctor (FD) to help achieve "Healthy China 2030" through providing continuous, comprehensive, and life-cycle contract services. However, there is a disparity between actual and targeted FD use, as residents continue to visit specialists in large hospitals. The government implemented initiatives to improve residents' willingness to sign up with and visit their FDs. Factors that influence contract behavior are therefore significant for frontier policy research. METHODS: Two survey waves were conducted in Shanghai (2013 and 2016). The first wave included 2754 people and the second 1995 people. Exploratory factor analysis was used to synthesize "satisfaction" as a predictor of contract behavior. Pearson's chi-square, pooled and logistic regression models were used to estimate associations between influencing factors and contract behavior, and clarify variations in factors across the two waves. RESULTS: Four factors were extracted from 15 satisfaction items: "Treatment Environment," "Medical Technology," "Service Specification" and "Service Attitude". Consistent with descriptive analysis, longitudinal analysis showed sociodemographic characteristics (age, education, marital status, and hukou) were significant predictors of contract behavior. The odds ratio of non-communicable diseases (NCD) patients for contract behavior was 2.218 times that of residents without NCD. Contract behavior was positively correlated with awareness of FD services (OR = 21.674, 95%CI = 15.043-31.229), satisfaction with Service Attitude (OR = 1.210, 95%CI = 1.009-1.451), and visit compliance (OR = 1.959, 95%CI = 1.564-2.452). Over time, the odds ratios of the married, Shanghai hukou, NCD, and awareness of FD services declined from 0.456, 1.795, 2.492, 28.690 to 0.443, 1.678, 1.910 and 14.031 respectively, while those of age, and visit compliance increased from 1.027, 1.521 to 1.041 and 2.305 respectively. In 2016, an education-contract gradient had formed (the higher the education level, the higher probability of signing with a FD), whereas high school education had the highest odds ratio (OR = 1.163,95%CI = 0.740-1.827) in 2013. Service Attitude was the only significant satisfaction-related predictor (OR = 1.358, 95%CI = 1.001-1.842) in 2016, compared with "Treatment Environment" (OR = 1.224, 95%CI = 1.001-1.496) and "Service Specification" in 2013(OR = 1.270, 95%CI = 1.040-1.552). CONCLUSIONS: Except for the socio-demographic variables, NCD, awareness of FD services, satisfaction and visit compliance were significant predictors of contract behavior with FDs. The effect of visit compliance had increased over time while NCD and awareness of FD services were losing impact over time. Significant satisfaction factors had also changed from "Treatment Environment" and "Service Specification" to "Service Attitude".


Assuntos
Serviços de Saúde Comunitária , Medicina de Família e Comunidade , Adolescente , Adulto , Idoso , China , Serviços de Saúde Comunitária/métodos , Serviços Contratados , Medicina de Família e Comunidade/métodos , Feminino , Pesquisas sobre Serviços de Saúde , Humanos , Modelos Logísticos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Satisfação Pessoal , Adulto Jovem
14.
Biomed Res Int ; 2018: 6062520, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30246024

RESUMO

Ubiquitin and ubiquitin like proteins (UBLs) play key roles in eukaryotes. These proteins are attached to their target proteins through an E1-E2-E3 cascade and modify the functions of these proteins. Since the discovery of ubiquitin, several UBLs have been identified, including Nedd8, SUMO, ISG15, and Atg8. Ubiquitin and UBLs share a similar three-dimensional structure: ß-grasp fold and an X-X-[R/A/E/K]-X-X-[G/X]-G motif at the C-terminus. We have previously reported that ubiquitin, Nedd8, and SUMO mimicking peptides which all contain the conserved motif X-X-[R/A/E/K]-X-X-[G/X]-G still retained their reactivity toward their corresponding E1, E2, and E3 enzymes. In our current study, we investigated whether such C-terminal peptides could still be transferred onto related pathway enzymes to probe the function of these enzymes when they are fused with a protein. By bioinformatic search of protein databases, we selected eight proteins carrying the X-X-[R/A/E/K]-X-X-[G/X]-G motif at the C-terminus of the ß-grasp fold. We synthesized the C-terminal sequences of these candidates as short peptides and found that three of them showed significant reactivity with the ubiquitin E1 enzyme Ube1. We next fused the three reactive short peptides to three different protein frames, including their respective native protein frames, a ubiquitin frame and a peptidyl carrier protein (PCP) frame, and measured the reactivities of these peptide-fused proteins with Ube1. Peptide-fused proteins on ubiquitin and PCP frames showed obvious reactivity with Ube1. However, when we measured E2 UbcH7 transfer, we found that the PCP-peptide fusions lost their reactivity with UbcH7. Taken together, these results suggested that the recognition of E2 enzymes with peptide-fused proteins depended not only on the C-terminal sequences of the ubiquitin-mimicking peptides, but also on the overall structures of the protein frames.


Assuntos
Peptídeos/metabolismo , Enzimas Ativadoras de Ubiquitina/metabolismo , Enzimas de Conjugação de Ubiquitina/metabolismo , Proteínas , Ubiquitina , Ubiquitinas
15.
Clin Chim Acta ; 486: 26-33, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30025752

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) have been proven to be involved in carcinogenesis and to be released into peripheral blood. Our objective was to develop a circulating lncRNA as a novel biomarker to predict lung cancer. METHODS: We analyzed the lncRNA expression profile in lung cancer patients by lncRNA array and identified lncRNA XLOC_009167 as a circulating biomarker using qRT-PCR in whole blood of lung cancer patients. The diagnostic value of was analyzed by area under curve (AUC) and the receiver operating characteristic (ROC) test. RESULTS: LncRNA XLOC_009167 was screened as a candidate biomarker for lung cancer and was up-regulated in both lung cancer tissues and cell lines. Notably, lncRNA XLOC_009167 in whole blood of lung cancer patients was highly expressed as compared with that in healthy controls or in patients with pneumonia. The values of AUC of lung cancer vs. healthy controls, and that of lung cancer vs. pneumonia were 0.7398 (95%CI = 0.6493 to 0.8303) and 0.7005 (95%CI = 0.5771 to 0.8240), respectively. Intriguingly, the ROC showed lncRNA XLOC_009167 was a better diagnostic potential compared to the traditional biomarkers (CYFR21-1, NSE and CA72-4), and the circulating lncRNA XLOC_009167 was found to be stable in whole blood under different conditions. CONCLUSIONS: LncRNA XLOC_009167 could serve as a novel diagnostic biomarker to distinguish lung cancer from benign lung disease and healthy controls.


Assuntos
Biomarcadores Tumorais/sangue , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/diagnóstico , RNA Longo não Codificante/sangue , Biomarcadores Tumorais/genética , Feminino , Humanos , Neoplasias Pulmonares/genética , Masculino , Pessoa de Meia-Idade , RNA Longo não Codificante/genética , RNA Longo não Codificante/isolamento & purificação
16.
Water Sci Technol ; 77(9-10): 2509-2516, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29893740

RESUMO

The effects of Mn(II) on Fenton system to treat papermaking wastewater and the mechanism of Mn(II) enhanced Fenton reaction were investigated in this study. The chemical oxygen demand (COD) removal efficiency was enhanced in the presence of Mn(II), which increased by 19% compared with that of the Fenton system alone. The pseudo-first order reaction kinetic rate constant of Mn(II)/Fenton system was 2.11 times higher than that of Fenton system. 67%-81% COD were removed with the increasing Mn(II) concentration from 0 to 0.8 g/L. COD removal efficiency was also enhanced in a wider pH range (3-7), which indicated the operation parameters of Fenton technology could be broadened to a milder condition. The study of the mechanism showed that Mn(II) participated in the oxidation and coagulation stages in Fenton system. In the oxidation stage, Mn(II) promotes the production of HO2•/ O2•-, then HO2•/ O2•- reacts with Fe(III) to accelerate the formation of Fe(II), and finally accelerates the production of HO•. Meantime MnMnO3 and Fe(OH)3 forms in the coagulation stage, facilitating the removal of suspended substances and a large amount of COD, which enhances the overall COD removal of papermaking wastewater. This study provided a detailed mechanism to improve practical applications of Fenton technology.


Assuntos
Manganês/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/química , Purificação da Água/métodos , Análise da Demanda Biológica de Oxigênio , Peróxido de Hidrogênio/química , Resíduos Industriais , Ferro/química , Cinética , Oxirredução , Papel
17.
Mol Genet Genomics ; 293(5): 1191-1204, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29869696

RESUMO

Cellulose is a major component of plant cell walls and is necessary for plant morphogenesis and biomass. COBL (COBRA-Like) proteins have been shown to be key regulators in the orientation of cell expansion and cellulose crystallinity status. To clarify the role of a cotton COBL gene, GhCOBL9A, we conducted the ectopic expression and functional analysis in Arabidopsis. Previous study showed that GhCOBL9A was preferentially expressed during secondary cell wall biosynthesis in cotton fibers, and showed a significant co-expression pattern with cellulose synthase genes. Here, we detected that overexpression of GhCOBL9A induced the up-regulation of genes related to cellulose synthesis and enhanced the cellulose deposition. As a result, GhCOBL9A transgenic plants displayed increased hypocotyl and root lengths in early development, and cell wall thickening at the SCW stage. Notably, overexpression of GhCOBL9A led to an erect, robust-stature phenotype and brought higher biomass in mature plants. In addition, overexpression of GhCOBL9A in Arabidopsis AtCOBL4 mutants, a paralogous gene of GhCOBL9A, also led to a stronger growth potential, but the Atcobl4 mutant phenotype could not be rescued, implying the functional divergence of GhCOBL9A and AtCOBL4 paralogs. Taken together, these results suggest that overexpression of GhCOBL9A contributes to plant cell elongation and thickening, and increased biomass, which provides references for further utilizing GhCOBL9A to improve yield and quality traits in cotton and other species.


Assuntos
Arabidopsis/crescimento & desenvolvimento , Parede Celular/metabolismo , Gossypium/crescimento & desenvolvimento , Desenvolvimento Vegetal , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Celulose/metabolismo , Regulação da Expressão Gênica de Plantas , Glicosilfosfatidilinositóis/metabolismo , Gossypium/genética , Gossypium/metabolismo , Proteínas de Plantas/genética , Caules de Planta/genética , Caules de Planta/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Xilema/genética , Xilema/metabolismo
18.
Ann Thorac Surg ; 106(3): 888-894, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29763594

RESUMO

BACKGROUND: Paravertebral block (PVB) has been proven to be an efficient way to control postoperative pain in patients who have undergone a thoracotomy. This study explored whether the use of a patient-controlled PVB can provide benefits over intravenous patient-controlled analgesia (PCA) for 3-port single-intercostal video-assisted thoracic surgery. METHODS: From May 2015 to December 2016, patients who had solitary pulmonary nodules or spontaneous pneumothorax and underwent single-intercostal video-assisted thoracic surgery were randomly allocated to receive patient-controlled PVB or intravenous PCA. Intramuscular dezocine (10 mg) was used as a rescue medication. None of the surgeons, patients, or investigators assessing outcomes or analyzing the data were blinded to the group assignments. Pain level was measured by the visual analog score. RESULTS: There were 86 patients assigned to the PVB group and 85 patients assigned to the PCA group. The difference in the mean visual analog score between these two groups was not significant (p = 0.115). For patients who needed rescue medication, the cumulative dezocine dose in the PVB group was significantly lower than that in the PCA group (21.7 mg vs 30.9 mg, p = 0.001) throughout the 4 postoperative days. The frequencies of severe vomiting (p = 0.003) and hypotension (p = 0.005) were significantly lower in the PVB group. CONCLUSIONS: PVB, which resulted in lower cumulative dezocine doses and produced fewer side effects than PCA, can provide effective pain relief for patients undergoing video-assisted thoracic surgery.


Assuntos
Analgesia Controlada pelo Paciente/métodos , Raquianestesia/métodos , Neoplasias Pulmonares/cirurgia , Medição da Dor , Nódulo Pulmonar Solitário/cirurgia , Cirurgia Torácica Vídeoassistida/métodos , Anestésicos Intravenosos , Feminino , Seguimentos , Humanos , Neoplasias Pulmonares/patologia , Masculino , Bloqueio Nervoso/métodos , Manejo da Dor/métodos , Satisfação do Paciente/estatística & dados numéricos , Pneumotórax/patologia , Pneumotórax/cirurgia , Estudos Prospectivos , Medição de Risco , Nódulo Pulmonar Solitário/patologia , Estatísticas não Paramétricas , Toracotomia/métodos , Resultado do Tratamento
19.
Chem Sci ; 9(5): 1375-1384, 2018 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-29675186

RESUMO

The development of highly efficient, low-cost and stable electrocatalysts for overall water splitting is highly desirable for the storage of intermittent solar energy and wind energy sources. Herein, we show for the first time that nickel can be extracted from NiFe-layered double hydroxide (NiFe-LDH) to generate an Ni2P@FePO x heterostructure. The Ni2P@FePO x heterostructure was converted to an Ni2P@NiFe hydroxide heterostructure (P-NiFe) during water splitting, which displays high electrocatalytic performance for both the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) in 1.0 M KOH solution, with an overpotential of 75 mV at 10 mA cm-2 for HER, and overpotentials of 205, 230 and 430 mV at 10, 100 and 1000 mA cm-2 for OER, respectively. Moreover, it could afford a stable current density of 10 mA cm-2 for overall water splitting at 1.51 V in 1.0 M KOH with long-term durability (100 h). This cell voltage is among the best reported values for bifunctional electrocatalysts. The results of theoretical calculations demonstrate that P-NiFe displays optimized adsorption energies for both HER and OER intermediates at the nickel active sites, thus dramatically enhancing its electrocatalytic activity.

20.
Adv Colloid Interface Sci ; 256: 340-351, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29530273

RESUMO

Anisotropic surface properties of minerals play an important role in a variety of fields. With a focus on the two most intensively investigated silicate minerals (i.e., phyllosilicate minerals and pegmatite aluminosilicate minerals), this review highlights the research on their anisotropic surface properties based on their crystal structures. Four surface features comprise the anisotropic surface chemistry of minerals: broken bonds, energy, wettability, and charge. Analysis of surface broken bond and energy anisotropy helps to explain the cleavage and growth properties of mineral crystals, and understanding surface wettability and charge anisotropy is critical to the analysis of minerals' solution behavior, such as their flotation performance and rheological properties. In a specific reaction, the anisotropic surface properties of minerals are reflected in the adsorption strengths of reagents on different mineral surfaces. Combined with the knowledge of mineral crushing and grinding, a thorough understanding of the anisotropic surface chemistry properties and the anisotropic adsorption behavior of minerals will lead to the development of effective relational models comprising their crystal structure, surface chemistry properties, and targeted reagent adsorption. Overall, such a comprehensive approach is expected to firmly establish the connection between selective cleavage of mineral crystals for desired surfaces and designing novel reagents selectively adsorbed on the mineral surfaces. As tools to characterize the anisotropic surface chemistry properties of minerals, DLVO theory, atomic force microscopy (AFM), and molecular dynamics (MD) simulations are also reviewed.

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