Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 967
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
FASEB J ; 34(1): 1169-1181, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914631

RESUMO

BHLHE40, a member of the basic helix-loop-helix transcription factor family, has been reported to play an important role in inflammatory diseases. However, the regulation and function of BHLHE40 in Helicobacter pylori (H pylori)-associated gastritis is unknown. We observed that gastric BHLHE40 was significantly elevated in patients and mice with H pylori infection. Then, we demonstrate that H pylori-infected GECs express BHLHE40 via cagA-ERK pathway. BHLHE40 translocates to cell nucleus, and then binds to cagA protein-activated p-STAT3 (Tyr705). The complex increases chemotactic factor CXCL12 expression (production). Release of CXCL12 from GECs fosters CD4+ T cell infiltration in the gastric mucosa. Our results identify the cagA-BHLHE40-CXCL12 axis that contributes to inflammatory response in gastric mucosa during H pylori infection.

2.
Adv Exp Med Biol ; 1217: 61-78, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31898222

RESUMO

The cullin-RING ubiquitin ligases comprise the largest subfamily of ubiquitin ligases. They control ubiquitylation and degradation of a large number of protein substrates in eukaryotes. p97 is an ATPase domain-containing protein segregase. It plays essential roles in post-ubiquitylational events in the ubiquitin-proteasome pathway. Together with its cofactors, p97 collaborates with ubiquitin ligases to extract ubiquitylated substrates and deliver them to the proteasome for proteolysis. Here we review the structure, functions, and mechanisms of p97 in cellular protein degradation in coordination with its cofactors and the cullin-RING ubiquitin ligases.

3.
Mol Oncol ; 2020 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-31899582

RESUMO

Oncolytic viruses armed with therapeutic transgenes of interest show great potential in cancer immunotherapy. Here, a novel oncolytic adenovirus carrying a signal regulatory protein-α (SIRPα)-IgG1Fc fusion gene (termed SG635-SF) was constructed, which could block the CD47 "don't eat me" signal of cancer cells. A strong promoter sequence (CCAU) was chosen to control the expression of the SF fusion protein, and a 5/35 chimeric fiber was utilized to enhance the efficiency of infection. As a result, SG635-SF was found to specifically proliferate in hTERT-positive cancer cells and largely increased the abundance of the SF gene. The SF fusion protein was effectively detected, and CD47 was successfully blocked in SK-OV3 and HO8910 ovarian cancer cells expressing high levels of CD47. Although the ability to induce cell cycle arrest and cell death was comparable to that of the control empty SG635 oncolytic adenovirus in vitro, the anti-tumor effect of SG635-SF was significantly superior to that of SG635 in vivo. Furthermore, CD47 was largely blocked and macrophage infiltration distinctly increased in xenograft tissues of SK-OV3 cells but not in those of CD47-negative HepG2 cells, indicating that the enhanced antitumor effect of SG635-SF was CD47-dependent. Collectively, these findings highlight a potent anti-tumor effect of SG635-SF in the treatment of CD47-positive cancers.

4.
Support Care Cancer ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31907651

RESUMO

PURPOSE: Exercise is beneficial for prostate cancer patients' physical functioning; however, effects on social and cognitive functioning are inconsistent. This meta-analysis of exercise interventions for prostate cancer patients had two aims: the primary aim was to evaluate the effects of exercise interventions on social functioning; the secondary aim was to consider additional outcomes of cognitive functioning as well as adverse events. METHODS: Electronic databases (Embase, MEDLINE, PubMed, PsycINFO, and the Chinese database Airti Library) were searched for relevant papers (1987-2019), which included hand searching. After careful inspection, 10 relevant randomized controlled trials were analyzed using Comprehensive Meta-Analysis software; pooled means determined social and cognitive functioning. RESULTS: Meta-analysis of summary scores (fixed-effects model) showed an overall beneficial effect of exercise on social functioning (Hedges' g = 0.35, 95% CI [0.193, 0.515], p < 0.001) and cognitive functioning (Hedges' g = 0.35, 95% CI [0.123, 0.575], p < 0.01) in men with prostate cancer when compared to controls. Intervention durations of 12-16 and 24-48 weeks that provided supervised aerobic exercise combined with resistance exercise sessions had a small to medium effect on social functioning compared to controls. One exercise group experienced one serious, but non-fatal, adverse event due to a higher exercise intensity (50-75% VO2max). DISCUSSION AND RECOMMENDATIONS: To the best of our knowledge, this is the first meta-analysis to examine the effects of exercise interventions on cognitive functioning among prostate cancer patients. We suggest further research be conducted to confirm these findings.

5.
Sex Transm Infect ; 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31919274

RESUMO

OBJECTIVES: Men who have sex with men (MSM) are at high risk of hepatitis C virus (HCV). This study investigated predictors of first-time HCV testing uptake during a 6-month period among a sample of MSM in Hong Kong. METHODS: Participants were 351 Chinese-speaking MSM who had never received HCV testing. Participants completed two telephone surveys 6 months apart. At baseline, participants reported on sociodemographics, sexual behaviours, risk perception, depressive symptoms and anxiety symptoms. Illness representations, which refers to how people think about HCV, was measured by the Brief Illness Perception Questionnaire (BIPQ) at baseline. The BIPQ assessed identity (identifying symptoms of HCV), timeline (whether HCV is acute/chronic), consequences (severity of HCV), personal control and treatment control (whether HCV is under volitional control), concern, emotions (anger, guilt or shame) and coherence (overall comprehensibility of HCV). Six months later, participants reported on HCV testing uptake. Logistic regression was used to analyse the associations between baseline predictors and HCV testing uptake. RESULTS: Most participants were aged ≤30 years (55.0%) and had attained college education or above (85.2%). Among 242 participants (68.9%) who completed the month 6 follow-up, 12.4% had tested for HCV during the follow-up period. After adjustment for HIV testing and chemsex in the last year, participants who perceived more severe consequences of HCV reported higher HCV testing uptake (adjusted ORs (AOR): 2.22, 95% CI: 1.65 to 3.00). Belief that treatment can control HCV (AOR: 1.75, 95% CI: 1.35 to 2.26) and having negative emotions related to HCV (AOR: 1.59, 95% CI: 1.25 to 2.03) were also positively associated with HCV testing uptake. CONCLUSIONS: Targeted health promotion efforts are needed to increase HCV testing among MSM in Hong Kong. Healthcare workers engaged in HCV-related programming should consider modifying the illness representations of HCV. Integrating HCV and HIV testing services may also be beneficial.

6.
Sensors (Basel) ; 20(2)2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31936499

RESUMO

An environmental thermal comfort model has previously been quantified based on the predicted mean vote (PMV) and the physical sensors parameters, such as temperature, relative humidity, and air speed in the indoor environment. However, first, the relationship between environmental factors and physiology parameters of the model is not well investigated in the smart home domain. Second, the model that is not mainly for an individual human model leads to the failure of the thermal comfort system to fulfill the human's comfort preference. In this paper, a cyber-physical human centric system (CPHCS) framework is proposed to take advantage of individual human thermal comfort to improve the human's thermal comfort level while optimizing the energy consumption at the same time. Besides that, the physiology parameter from the heart rate is well-studied, and its correlation with the environmental factors, i.e., PMV, air speed, temperature, and relative humidity are deeply investigated to reveal the human thermal comfort level of the existing energy efficient thermal comfort control (EETCC) system in the smart home environment. Experimental results reveal that there is a tight correlation between the environmental factors and the physiology parameter (i.e., heart rate) in the aspect of system operational and human perception. Furthermore, this paper also concludes that the current EETCC system is unable to provide the precise need for thermal comfort to the human's preference.

7.
J Am Chem Soc ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31948234

RESUMO

The black crystalline (aza)triangulene-based covalent organic framework (TANG-COF) was synthesized from trinitro-TANG precursor via one-pot-two-step reaction involving Pd-catalyzed hydrogenation and polycondensation with an aromatic dial-dehyde. High crystallinity and permanent porosity of the layered two-dimensional (2D) structure were established. The rigid, electron-rich TANG building block enables strong π-electron interactions manifested in broad absorptions across the visible and NIR region (Eg ~1.2 eV). The high HOMO of TANG-COF (-4.8 eV) level enables facile p-doping resulting in electrical con-ductivity (up to 10-2 S/cm) and room-temperature paramagnetic behavior with spin concentration ~10%. DFT calculations reveal a dispersion of the highest occupied band, both within the 2D polymer layers (0.28 eV) and along their π-stacked di-rection (0.95 eV).

8.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(1): 47-52, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31948524

RESUMO

OBJECTIVE: To study the influence of dasatinib treatment on body height in children with acute myeloid leukemia (AML). METHODS: A retrospective analysis was performed for the clinical data of 86 AML children aged <17 years. According to the treatment regimen, these children were divided into a conventional chemotherapy group and a dasatinib chemotherapy group. The 57 children in the conventional chemotherapy group were given conventional chemotherapy drugs without tyrosine kinase inhibitor, and the 29 children in the dasatinib chemotherapy group were given conventional chemotherapy drugs and dasatinib. The two groups were compared in terms of height standard deviation score (HtSDS) at the beginning of treatment and after treatment, as well as the change in HtSDS after 1 and 2 years of treatment. RESULTS: There was no significant difference in HtSDS between the conventional and dasatinib chemotherapy groups before treatment. Within the first two years of treatment, the dasatinib chemotherapy group had a similar change trend of HtSDS as the conventional chemotherapy group. Four children in the dasatinib chemotherapy group reached the final adult height during follow-up, which was significantly lower than the target height (P=0.044). In the conventional chemotherapy group, there was no significant difference between final adult height and target height. In the dasatinib chemotherapy group, the children in adolescence had a significant change in HtSDS after treatment (P=0.032). CONCLUSIONS: Dasatinib treatment may affect the final height of children with AML, and the use of dasatinib after the beginning of adolescence may lead to growth disorder, but dasatinib treatment has little effect on body height in the short-term treatment.

9.
Zhongguo Fei Ai Za Zhi ; 23(1): 5-14, 2020 Jan 20.
Artigo em Chinês | MEDLINE | ID: mdl-31948532

RESUMO

BACKGROUND: Early investigation suggested patients' level of awareness regarding clinical trials was related with willingness to participation. This study was intended to evaluate the level of awareness of cancer patients regarding clinical trials and related influencing factors, and to compare the differences of awareness between patients who attended clinical trials before and not. METHODS: From Jun, 2018 to April, 2019, standardized question-naires were gathered from cancer patients (attended clinical trials vs not attended clinical trials) in our hospital regarding basic information and 10 other questions about awareness. The level of awareness was evaluated and patients were classified into "low cognition" and "high cognition" groups. Logistic regression analysis was performed to determine whether certain characteristics would predict for awareness. RESULTS: Of the 617 participants, 38.6% have attended clinical trials before. 338 (54.6%) patients had a correct overall understanding of clinical trials, while 44 (7.1%) patients still thought participants were the victim of scientific research. Except for the compensation of medical expenses (51.5% vs 48.7%) and related laws of clinical trials (52.3% vs 45.5%), other parts of understanding were elevated in patients attended clinical trials before comparing with patients who didn't, including significance (86.2% vs 77.6%), risk disclosure (91.2% vs 71.6%), confidentiality (73.2% vs 59.7%), voluntariness (95.8% vs 76.3%), withdrawal (86.6% vs 68.2%) and expenses (62.8% vs 39.2%). The proportion of participants who understand these components did not increase even in 239 patients who had attended clinical trials before. Participants who attended clinical trials before (OR=1.83, 95%CI: 1.11-3.00), unmarried/divorced (OR=5.04, 95%CI: 1.73-14.66), retired (OR=2.53, 95%CI: 1.16-5.50) had a higher level of awareness, while patients who had bad impression with doctors (OR=0.43, 95%CI: 0.26-0.72) had lower awareness. CONCLUSIONS: The current level of awareness for clinical trials of cancer patients in our hospital was relatively low, even in patients who had attended clinical trials before. It's necessary to improve patients' awareness of clinical trial by promoting harmony relationship between patients and doctors, as well as by enhancing related propagation. Strengthening the adequacy and efficacy of informed consent in clinical trials also needs to be achieved in the future.

10.
Zhongguo Fei Ai Za Zhi ; 23(1): 41-49, 2020 Jan 20.
Artigo em Chinês | MEDLINE | ID: mdl-31948537

RESUMO

BACKGROUND: The clinical trials of new anti-tumor drugs are prospering in China. The acceptance of clinical trials in patients is an important factor affecting the speed and quality of clinical trials. Previous studies have investigated the acceptance of clinical trials in those cancer patients, who have never participated in a trial. This study is designed to investigate and compare the acceptance and related causes of clinical trials in cancer patients who have once participated in a clinical trial or not. METHODS: From June 2018 to April 2019, a standardized questionnaire-based survey was conducted among two groups of cancer patients classified by history of clinical trial participation in Cancer hospital, Chinese Academy of Medical Science, mainly focusing on their overall acceptance of clinical trials and related considerations, including the role of attending doctors, as well as group differences between the two participants. RESULTS: A total of 538 patients were enrolled with an average age of 53.5 years old, 51.1% of whom were males, and 43.3% of whom have never participated in a clinical trial. Overall, 502 patients (93.3%) were willing to or recommend their relatives or friends to participate in clinical trials, and patients with history of clinical trial participation had higher willingness (96.6% vs 90.8%, P=0.008). Patients were most likely to be motivated by expectation of optimal treatment (100.0% vs 99.3%) for both those who had once participated in a clinical trial or those not, respectively followed by financial burden reduction (56.0%) and recommendation by attending doctor (43.7%). The main reasons for unwillingness-to-participate for those who had once participated in a clinical trial were abandoning other treatment options, divided into control group or additional visits, while for those who had never participated in a clinical trial, ineffective treatment or serious adverse reactions were their main concerns. In the decision-making of clinical trial participation, 88% patients highly valued the role of recommendation by attending doctors. Among patients without trial participation history, 60.9% of those had no unwillingness-to-participate expressed that recommendation by attending doctors would change their decisions. The study also reported patients' preferences for information and access to clinical trials. CONCLUSIONS: The acceptance of clinical trials in cancer patients in our hospital is generally high, especially in patients who had a history of trial participation. It's of substantial significance to give full play to the role of doctors in improving the acceptance of clinical trials of cancer patients in China.

11.
Dev Comp Immunol ; : 103611, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31953153

RESUMO

In grass carp (Ctenopharyngodon idella), septicemia is a systemic inflammatory response to bacterial infection. Once infected bacteria, a hyper-inflammatory state that could lead to septic shock and death. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. Ctenopharyngodon idella growth arrest and DNA damage-inducible 45 ba and bb (CiGadd45ba and CiGadd45bb) are two subtypes of Gadd45b. In the present study, miR-148 was confirmed to be involved in the inflammatory response after infection with Aeromonas hydrophila. Dual-luciferase reporter assays and miRNA expression profiling confirmed that miR-148 targeted both CiGadd45ba and CiGadd45bb. Transfection with miR148 mimics and inhibitors altered the expression levels of proinflammatory genes, suggesting that miRNAs regulate the immune response in grass carp. Our results provide a theoretical basis for studying the molecular mechanism underlying the regulation of inflammation by miR-148 in grass carp.

12.
FASEB J ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31944405

RESUMO

Mangrove-derived actinobacteria strains are well-known for producing novel secondary metabolites. The polycyclic tetramate macrolactam (PTM), ikarugamycin (IKA) isolated from Streptomyces xiamenensis 318, exhibits antiproliferative activities against pancreatic ductal adenocarcinoma (PDAC) in vitro. However, the protein target for bioactive IKA is unclear. In this study, whole transcriptome-based profiling revealed that the glycolysis pathway is significantly affected by IKA. Metabolomic studies demonstrated that IKA treatment induces a significant drop in glucose-6-phosphate and a slight increase in intracellular glucose level. Analysis of glucose consumption, lactate production, and the extracellular acidification rate confirmed the inhibitory role of IKA on the glycolytic flux in PDAC cells. Surface plasmon resonance (SPR) experiments and docking studies identified the key enzyme of glycolysis, hexokinase 2 (HK2), as a molecular target of IKA. Moreover, IKA reduced tumor size without overt cytotoxicity in mice with PDAC xenografts and increased chemotherapy response to gemcitabine in PDAC cells in vitro. Taken together, IKA can block glycolysis in pancreatic cancer by targeting HK2, which may be a potential drug candidate for PDAC treatment.

13.
Ying Yong Sheng Tai Xue Bao ; 31(1): 25-34, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31957377

RESUMO

To examine the effects of management measures on carbon and nitrogen contents, as well as their distribution and structural characteristics of different soil fractions in Moso bamboo plantations, we compared three types of the bamboo forests (undisturbed, extensively managed, and intensively managed) and the control secondary broadleaved evergreen forest using the methods of physical fractionation, chemical and biological analysis and Fourier-transform infrared spectroscopy (FTIR). The results showed that soil total organic carbon (TOC) and total nitrogen (TN) content, as well as free particulate organic carbon and nitrogen, soluble organic carbon and nitrogen (DOC, DON), and mineral-associated organic carbon and nitrogen in the undisturbed and extensively managed Moso bamboo plantations were significantly increased compared with that in the control. The distribution ratio of free particulate organic carbon and nitrogen in the undisturbed Moso bamboo plantation significantly increased, with mineral-associated organic carbon being the largest reservoir of soil organic carbon (67.6%). Intensive management resulted in the decrease of soil organic carbon, total nitrogen storage, and the contents of each component, but significantly increased DOC/TOC, the ratio of microbial biomass nitrogen to TN as well as the ratio of microbial biomass carbon to TOC (microbial quotient). Management measures significantly affected the chemical structure of SOC. Compared with the control, the relative intensities of phenolic and alcoholic-OH, aliphatic methyl and methylene, aromatic C=C, and carbonyl C=O absorption were higher in the SOC of undisturbed and extensively managed Moso bamboo plantations, and soil hydrophobicity was significantly increased. Results from correlation analysis showed that soil hydrophobicity and the content of aliphatic and aromatic groups were negatively correlated with microbial quotient and positively correlated with TOC and TN content. In conclusion, the increased inputs of organic matter residues (such as litter and roots) could contribute to the relative accumulation of chemical resistance compounds with reduced human disturbance, which significantly enhanced chemical stability of soil organic carbon. Soil clay minerals played a key role in protecting soil organic carbon through the formation of mineral-organic compounds, which facilitate the stability of soil carbon storage and the long-term preservation of soil carbon.

14.
Artigo em Inglês | MEDLINE | ID: mdl-31811037

RESUMO

Some chlorine resistant E. coli isolates harbor the locus of heat resistance (LHR), a genomic island conferring heat resistance. In this study, the protective effect of the LHR to cells challenged by chlorine and oxidative stress was quantified. Cloning of the LHR protected against NaClO (32 mM, 5 min), H2O2 (120 mM, 5 min) and peroxyacetic acid (105 mg/L, 5 min) but not against 5.8 mM KIO4, 10 mM acrolein and 75 mg/L allyl isothiocyanate. The lethality of oxidizing treatments against LHR-negative strains of E. coli was about 2 log10 (CFU/mL) higher when compared to LHR-positive strains of E. coli Oxidation of cytoplasmic proteins and membrane lipids was quantified with the fusion probe roGFP2-Orp1 and the fluorescent probe BODIPY581/591, respectively. The fragment of the LHR encoding for heat shock proteins protected cytoplasmic proteins but not membrane lipids against oxidation. The middle fragment of the LHR protected against the oxidation of membrane lipids but not of cytoplasmic proteins. Addition of H2O2, NaClO and peroxyacetic acid also induced GFP expression in the oxidation-sensitive reporter strain E. coli O104:H4Δstx2::gfp::amp r Cloning of pLHR reduced phage induction in E. coli O104:H4Δstx2::gfp::amp r after treatment with oxidizing chemicals. Screening of 160 strains of STEC revealed that none of the 160 strains of STEC harbors the LHR, additionally suggesting that the LHR and Stx-prophages are exclusive. Taken together, the contribution of LHR to the resistance to chlorine and oxidative stress is based on protection of multiple cellular targets by different proteins encoded by the genetic island.Importance. Chlorine treatments are used in water and waste water sanitation; the resistance of Escherichia coli to chlorine is thus of concern to public health. We show that a genetic island termed locus of heat resistance (LHR) not only protects E. coli against heat but also against chlorine and other oxidizing chemicals, adding to our knowledge of the tools used by E. coli to resist stress. Specific detection of the oxidation of different cellular targets in combination with cloning of fragments of the LHR provided insight into mechanisms of protection, and demonstrated that different fragments of the LHR protect different cellular targets. In E. coli, presence of the LHR virtually excluded other virulence factors. It is tempting to speculate that the LHR is maintained by strains of E. coli with an environmental lifestyle but excluded by pathogenic strains that adapted to interact with vertebrate hosts.

15.
Am J Med Sci ; 358(6): 400-411, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31813467

RESUMO

BACKGROUND: Many studies have investigated the efficacy and safety of lentinan combined with cisplatin versus cisplatin alone for controlling malignant pleural effusion (MPE). This study is a meta-analysis of available evidence. MATERIALS AND METHODS: Seventeen studies reporting lentinan combined with cisplatin versus cisplatin alone for controlling MPE were reviewed. Pooled odds ratios and hazard ratio with 95% confidence intervals were calculated using the fixed effects model of meta-analysis. RESULTS: The overall response rate (ORR) of lentinan combined with cisplatin for controlling MPE was significantly higher than that of cisplatin alone (P < 0.001). In addition, lentinan combined with cisplatin showed a better benefit of quality of life (QOL) compared with cisplatin alone (P < 0.001). The main adverse effects (AEs) found in the control plan were hematological reactions, nausea/vomiting, chest pain and fever. However, the presence of lentinan did not have an extra influence on the incidence of AEs (P > 0.05). CONCLUSIONS: Intrapleural injection of lentinan combined with cisplatin had a better benefit of ORR and QOL for controlling MPE, compared with cisplatin alone. Moreover, lentinan combined with cisplatin had a similar incidence of AEs with cisplatin alone.

16.
Onco Targets Ther ; 12: 9449-9458, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819476

RESUMO

Background: Substantive studies have described the ectopic microRNAs as a determinant of the pathogenesis of endometrial cancer (EC). miR-214-3p has been reported to be significantly downregulated in EC tissues, and its overexpression has been shown to inhibit the proliferation, migration, and invasion of EC cells. Our study sought to explore the molecular mechanism underlying the inhibitory effect of miR-214-3p on metastasis of EC cells. Methods: The expressions of miR-214-3p and TWIST1 in EC tissues and cells were detected by quantitative real-time PCR. Cell migration, invasion, and epithelial-to-mesenchymal transition (EMT) were measured by transwell and Western blot analyses, respectively. The interaction between miR-214-3p and TWIST1 was confirmed by luciferase reporter assay. Xenograft tumor assay was performed to verify the role and underlying mechanism of miR-214-3p in EC in vivo. Results: miR-214-3p was downregulated and TWIST1 was upregulated in EC tissues and cells. miR-214-3p was negatively correlated with TWIST1 expression in EC tissues. Overexpression of miR-214-3p suppressed migration, invasion, and EMT in EC cells. TWIST1 was identified as a target of miR-214-3p in EC cells, and its overexpression significantly restored the inhibitory effects of miR-214-3p on cell migration, invasion, and EMT while its knockdown remarkably abolished miR-214-3p inhibitor-mediated promotion of progression of EC cells. Additionally, addition of miR-214-3p inhibited tumor growth by regulating EMT in vivo. Conclusion: miR-214-3p suppressed the EMT and metastasis of EC cells by targeting TWIST1, providing a novel biomarker for treatment of EC.

17.
Cell Oncol (Dordr) ; 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31792888

RESUMO

The authors would like to correct the following panels in Figures.

18.
Mol Plant ; 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31794845

RESUMO

In plants, high disease resistance often results in a fitness penalty to plant growth. Therefore, breeding crops with a balanced yield and disease resistance has become a major challenge. Recently, microRNA (miRNA)-mediated R gene turnover has been shown to be a protective mechanism for plants to prevent autoimmunity in the absence of pathogens. However, whether these miRNAs play a role in plant growth and how miRNA-mediated R gene turnover responds to pathogen infection have been rarely explored. Here, we identified the Brassica miRNA, miR1885, targets both immune receptor gene and development-related gene for negative regulation through distinct modes of action. MiR1885 directly silences a TIR-NBS-LRR class of R gene BraTNL1 but represses the expression of photosynthesis-related gene BraCP24 through Trans-Acting Silencing (TAS) gene BraTIR1-mediated silencing. We found that, under natural conditions, miR1885 was kept in low levels to maintain normal development and basal immunity but peaked during the floral transition to promote flowering. Interestingly, upon Turnip mosaic virus (TuMV) infection, miR1885-dependent trans-acting silencing of BraCP24 was enhanced to speed up floral transition, whereas miR1885-mediated R gene turnover was overwhelmed by TuMV-induced BraTNL1 expression, reflecting an integrative regulation of the arms race between plants and pathogens. Collectively, our results demonstrate that a single Brassica miRNA dynamically regulates both innate immunity and plant growth and responds to viral infection, therefore demonstrating an integrative strategy for Brassica in modulating the interplay between growth, immunity and pathogen infection.

19.
J Hematol Oncol ; 12(1): 129, 2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31801559

RESUMO

Histone demethylase LSD1 plays key roles during carcinogenesis, targeting LSD1 is becoming an emerging option for the treatment of cancers. Numerous LSD1 inhibitors have been reported to date, some of them such as TCP, ORY-1001, GSK-2879552, IMG-7289, INCB059872, CC-90011, and ORY-2001 currently undergo clinical assessment for cancer therapy, particularly for small lung cancer cells (SCLC) and acute myeloid leukemia (AML). This review is to provide a comprehensive overview of LSD1 inhibitors in clinical trials including molecular mechanistic studies, clinical efficacy, adverse drug reactions, and PD/PK studies and offer prospects in this field.

20.
J Pain Res ; 12: 2999-3012, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31807057

RESUMO

Purpose: The purpose of this study was to explore the central analgesia mechanism of moxibustion for chronic inflammatory visceral pain (CIVP). Methods: A CIVP rat model was established by 2,4,6-trinitrobenzene sulfonic acid (TNBS) plus 50% ethanol via enema. The analgesic effect of moxibustion was evaluated using the abdominal withdrawal reflex (AWR), mechanical withdrawal threshold (MWT), and thermal withdrawal latency (TWL). The expression profile of phosphorylated proteins of the mitogen-activated protein kinase (MAPK) signaling pathway in the spinal cord was assayed by protein microarray. The differentially expressed proteins were examined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) for functional clusters and corresponding signaling pathways. Results: Moxibustion exerted a significant analgesic effect for CIVP rats, mainly presenting as a decrease in the AWR score (all P<0.01) under different levels of distending pressure and an increase in MWT and TWL thresholds (all P<0.05). Compared with the normal group, 76 proteins were upregulated while 15 were downregulated, and MAPK signaling pathway was activated in the model group. Compared with the model group, there were 53 downregulated and 38 upregulated proteins in the moxibustion group, and MAPK signaling pathway was inhibited. Fold change (FC)>1.3 or <0.77 was taken as the screening standard to define the differentially expressed proteins. Fifteen differentially expressed proteins upregulated in the model group were downregulated in the moxibustion group. GO analysis showed that the differentially expressed proteins mainly controlled cellular metabolism regulation, transportation, and stress reactions. KEGG analysis revealed that these differentially expressed proteins were mostly involved in the ERK, JNK, and p38 pathways, and the ERK pathway was predominant. Conclusion: Moxibustion mitigates CIVP in rats and inhibits the phosphorylation of proteins in the spinal MAPK signaling pathway. The analgesic effect of moxibustion may be associated with the regulation of the spinal MAPK signaling pathway.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA