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1.
BMC Vet Res ; 17(1): 49, 2021 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-33494772

RESUMO

BACKGROUND: Borrelia burgdorferi is the spirochete that causes Lyme Borreliosis (LB), which is a zoonotic tick-borne disease of humans and domestic animals. Hard ticks are obligate haematophagous ectoparasites that serve as vectors of Borrelia burgdorferi. Studies on the presence of Lyme borreliosis in Egyptian animals and associated ticks are scarce. METHODS: This study was conducted to detect B. burgdorferi in different tick vectors and animal hosts. Three hundred animals (dogs=100, cattle=100, and camels=100) were inspected for tick infestation. Blood samples from 160 tick-infested animals and their associated ticks (n=1025) were collected and examined for the infection with B. burgdorferi by polymerase chain reaction (PCR) and sequencing of the 16S rRNA gene. The identified tick species were characterized molecularly by PCR and sequencing of the ITS2 region. RESULTS: The overall tick infestation rate among examined animals was 78.33% (235/300). The rate of infestation was significantly higher in camels (90%), followed by cattle (76%) and dogs (69%); (P = 0.001). Rhipicephalus sanguineus, Rhipicephalus (Boophilus) annulatus, and both Hyalomma dromedarii and Amblyomma variegatum, were morphologically identified from infested dogs, cattle, and camels; respectively. Molecular characterization of ticks using the ITS2 region confirmed the morphological identification, as well as displayed high similarities of R. sanguineus, H. dromedarii, and A. Variegatu with ticks identified in Egypt and various continents worldwide. Just one dog (1.67%) and its associated tick pool of R. sanguineus were positive for B. burgdorferi infection. The 16S rRNA gene sequence for B. burgdorferi in dog and R. sanguineus tick pool showed a 100% homology. CONCLUSION: Analyzed data revealed a relatively low rate of B. burgdorferi infection, but a significantly high prevalence of tick infestation among domesticated animals in Egypt, which possesses a potential animal and public health risk. Additionally, molecular characterization of ticks using the ITS2 region was a reliable tool to discriminate species of ticks and confirmed the morphological identification.

2.
Vet World ; 13(1): 68-72, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32158153

RESUMO

Background and Aim: One of the most common cutaneous infections seen in veterinary canine practice is canine demodicosis. Demodicosis is a parasitic skin infection with a possible impact on acute-phase proteins (APPs) and oxidant-antioxidant balance. This study aimed to estimate the possible alterations in hematological, biochemical, oxidant-antioxidant, and APP (C-reactive protein [CRP] and albumin) profiles in naturally infected dogs with demodicosis. Materials and Methods: This study enrolled 21 dogs that were divided into two groups: The control group including 7 apparently healthy dogs and the diseased group including 14 dogs with generalized demodicosis. Demodicosis was confirmed through microscopic detection. Blood samples were collected for the estimation of CBC, total protein, albumin, alanine transaminase, aspartate aminotransferase, blood urea nitrogen, creatinine, superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant capacity (TAC), catalase (CAT), malondialdehyde (MDA), and CRP levels. Results: Significant reduction in red blood cells along with significant elevation in white blood cells was recorded in the diseased group compared with the control group. There was also significant elevation in MDA, TAC, SOD, and CRP levels along with significant reduction in GSH-Px and CAT levels in the diseased group. Conclusion: Based on these findings, a relationship between canine generalized demodicosis and oxidant-antioxidant disequilibrium could be suggested. Evidence of this relation manifested in the elevation in MDA and SOD levels and reduction in GPx and CAT levels as a consequence to the release of ROS resulting from Demodex infection. CRP elevation is expected in canine demodicosis.

3.
J Urol ; 202(4): 826, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31172845
4.
Radiol Med ; 121(12): 935-943, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27586132

RESUMO

Lung imaging radiopharmaceuticals are helpful agents for measuring pulmonary blood flow and allow detection of pulmonary embolism and lung cancer. The goal of this study was to develop a novel potential radiopharmaceutical for lung imaging. Zolmitriptan (a selective serotonin receptor agonist) was successfully labeled with 99mTc via direct labeling method under reductive conditions studying different factors affecting the labeling efficiency. 99mTc-zolmitriptan was obtained with a maximum labeling yield of 92.5 ± 0.61 % and in vitro stability up to 24 h. Molecular modeling was done to predict the structure of 99mTc-zolmitriptan and ensure that radiolabeling did not affect binding ability of zolmitriptan to its receptor. Biodistribution studies showed that maximum lung uptake of 99mTc-zolmitriptan was 23.89 ± 1.2 % injected dose/g tissue at 15 min post-injection and retention in lungs remained high up to 1 h, whereas the clearance from mice appeared to proceed mainly via the renal pathway. Scintigraphic images confirmed the biodistribution results showing a high resolution lung image with low accumulation of radioactivity in other organs except kidneys and urinary bladder. 99mTc-zolmitriptan is not a blood product and so it is more safe than the currently available 99mTc-MAA, and its lung uptake is higher than that of the recently discovered 123I-IPMPD, 99mTc(CO)5I and 99mTc-DHPM. So, 99mTc-zolmitriptan could be used as a hopeful radiopharmaceutical for lung scintigraphic imaging.


Assuntos
Neoplasias Pulmonares/diagnóstico por imagem , Compostos de Organotecnécio/farmacocinética , Oxazolidinonas/farmacocinética , Cintilografia/instrumentação , Compostos Radiofarmacêuticos/farmacocinética , Triptaminas/farmacocinética , Animais , Concentração de Íons de Hidrogênio , Marcação por Isótopo , Masculino , Camundongos , Estrutura Molecular , Compostos de Organotecnécio/química , Oxazolidinonas/química , Compostos Radiofarmacêuticos/química , Pertecnetato Tc 99m de Sódio/química , Pertecnetato Tc 99m de Sódio/farmacocinética , Temperatura , Triptaminas/química
5.
East Mediterr Health J ; 20(1): 17-23, 2014 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-24932929

RESUMO

The national neonatal screening programme in the United Arab Emirates currently includes 16 disorders: congenital hypothyroidism, sickle-cell diseases, congenital adrenal hyperplasia, biotinidase deficiency and 12 amino acid, organic acid and fatty acid disorders. This paper reports data since the programme started in January 1995 up to December 2011 on the incidence of screened disorders and the molecular basis of positive screened cases. Screening used a combination of tandem mass spectrometry, molecular technologies and biochemical analysis. A total of 750 365 infants were screened and 717 babies saved from associated morbidity and/or mortality. The incidence of screened disorders were 1:1 873 for congenital hypothyroidism, 1:14 544 for phenylketonuria, 1:3 526 for amino acid, organic acid and fatty acid disorders, 1:9 030 for classical congenital adrenal hyperplasia, 1:8 300 for biotinidase deficiency, 1:2 384 for sickle-cell disease and 1:121 for sickle-cell traits. Coverage of neonatal screening in the population reached 95% in 2010.


Assuntos
Doenças do Recém-Nascido/diagnóstico , Programas de Rastreamento/organização & administração , Programas Nacionais de Saúde/organização & administração , Triagem Neonatal/organização & administração , Feminino , Humanos , Recém-Nascido , Doenças do Recém-Nascido/epidemiologia , Masculino , Desenvolvimento de Programas , Emirados Árabes Unidos/epidemiologia
6.
Vet Med Int ; 2014: 270345, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24693460

RESUMO

Background. Canine babesiosis is a clinically important hemoprotozoan parasite affecting dogs. The goal of this present study was to determine the clinical symptoms and to establish its hematological and microscopic detection and compare it with the PCR findings attained from dogs infected with Babesia canis vogeli. Methodology/Principal Findings. 13-PCR confirmed Babesia-infected dogs were examined; seminested PCR was used to discover the precise type of Babesia and Babesia canis vogeli was the only subspecies detected. The most consistent clinical signs were elevated rectal temperature and a pale mucous membrane. Thrombocytopenia, monocytosis, and lymphocytosis, along with a significant reduction in red cell parameters, were the most commonly recorded hematologic alterations. Microscopic examination revealed the presence of typical large merozoites and trophozoites of B. canis in the ratio 76.92%. Conclusions/Significance. The presumptive diagnosis of canine babesiosis should be based on a fever and anemia, while thrombocytopenia is considered the hallmark of the disease; microscopic examination may not be very revealing in the detection at low parasitemia, but it remains the most rapid confirmatory method. Seminested PCR turned out to be a sensitive and accurate method for diagnosis; during the process of differentiation between Babesia subspecies, only B. canis subsp. vogeli was detected.

7.
Climacteric ; 17(1): 55-9, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23647489

RESUMO

OBJECTIVE: To investigate whether three-dimensional saline-infusion transvaginal sonohysterography can replace hysteroscopy in the detection of intrauterine lesions in women with perimenopausal bleeding. MATERIALS AND METHODS: This study was carried out at the Department of Obstetrics and Gynecology, Menofyia University Hospital in Egypt. Fifty women who presented with perimenopausal bleeding and fulfilled the inclusion criteria were recruited for the study. Three-dimensional sonohysterography was performed followed by hysteroscopy for all women. Results were tabulated and statistically analyzed. The accuracy of both techniques was calculated in terms of sensitivity, specificity, positive and negative predictive values. RESULTS: The mean age of women enrolled was 47.5 ± 5.61 years and the mean body mass index was 28.13 ± 2.91 kg/m(2). Three-dimensional sonohysterography detected no abnormality in 26 women but hysteroscopy only detected 24 with no abnormality. Compared to hysteroscopy, three-dimensional sonohysterography has sensitivities of 100% and 92% in the detection of endometrial polyps and submucous fibroids, respectively. The specificity of sonohysterography for both lesions was 100%. The sensitivity and specificity for other lesions exceeded 95%. CONCLUSIONS: Three-dimensional sonohysterosonography may be considered as an effective alternative to hysteroscopy in the assessment of the uterine cavity for any woman with perimenopausal bleeding.


Assuntos
Histeroscopia , Menopausa , Ultrassonografia/métodos , Hemorragia Uterina/diagnóstico por imagem , Adulto , Índice de Massa Corporal , Feminino , Humanos , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Hemorragia Uterina/diagnóstico
8.
East Mediterr Health J ; 17(4): 266-70, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22259882

RESUMO

To determine the safety and efficacy of 2 consecutive doses of triclabendazole (TCBZ) in the treatment of Schistosoma mansoni infection in human cases infected with both S. mansoni and Fasciola sp., we conducted a field survey involving 6314 individuals from 15 villages. The overall prevalence of schistosomiasis alone was 15.8%, of fascioliasis alone 2.2%, and of combined infection 0.7%. Treatment with 2 doses of TCBZ was given to the 49 cases with combined infection. Eight weeks after treatment, the cure rate was 96% for fascioliasis and was 32.7% for schistosomiasis. All schistosomiasis cases cured had a low intensity infection. Liver function tests done before treatment and 8 weeks after substantiate the safety of 2 doses of TCBZ given to those with combined infection. Administration of TCBZ should precede praziquantel in treatment of combined infection, however TCBZ cannot be recommended for infection with S. mansoni alone.


Assuntos
Anti-Helmínticos/administração & dosagem , Benzimidazóis/administração & dosagem , Fasciolíase/tratamento farmacológico , Esquistossomose/tratamento farmacológico , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Egito , Fasciolíase/complicações , Feminino , Humanos , Testes de Função Hepática , Masculino , Pessoa de Meia-Idade , Contagem de Ovos de Parasitas , Schistosoma mansoni , Esquistossomose/complicações , Resultado do Tratamento , Triclabendazol
9.
East Mediterr Health J ; 16(4): 402-7, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20795424

RESUMO

This study assessed the knowledge and practices about folic acid in pregnancy among pregnant women attending 2 main maternal and child health centres in Abu Dhabi. The majority of the 277 interviewed mothers (79.1%) had heard of folic acid and 46.6% had accurate knowledge about the role of folate in preventing neural tube defects. There were good practices regarding folate supplementation in the current pregnancy; most of the interviewed mothers took it daily and in the recommended dose. However, only a minority took it prior to pregnancy. Education, irrespective of age or parity, was the major factor determining better knowledge of folic acid in pregnancy.


Assuntos
Atitude Frente a Saúde , Ácido Fólico/uso terapêutico , Conhecimentos, Atitudes e Prática em Saúde , Defeitos do Tubo Neural/prevenção & controle , Gestantes/psicologia , Complexo Vitamínico B/uso terapêutico , Adulto , Escolaridade , Comportamento Alimentar , Feminino , Humanos , Modelos Logísticos , Paridade , Cuidado Pré-Concepcional , Gravidez , Cuidado Pré-Natal , Automedicação , Inquéritos e Questionários , Emirados Árabes Unidos , População Urbana
10.
East Mediterr Health J ; 16(9): 932-6, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21218718

RESUMO

The present study assessed the schistosomicidal and fasciolicidal actions of the myrrh-derivative Mirazid in an area of low schistosomiasis transmission. A total of 27 patients infected with Schistosoma mansoni and 16 with Fasciola spp. received the maximum recommended dose of Mirazid. Pretreatment egg counts in 4 Kato-Katz slides were compared with similar counts in stool samples collected 1 and 2 months after treatment. Standard procedures and quality control measures were followed. The results revealed that Mirazid used as schistosomicidal or fasciolicidal agent in the maximum recommended dose has a low cure rate and produced a negligible reduction in egg counts. Prescribing such an ineffective drug in Egypt might endanger the achievements of the schistosomiasis control strategy.


Assuntos
Fasciolíase/tratamento farmacológico , Fitoterapia/métodos , Extratos Vegetais/uso terapêutico , Esquistossomose/tratamento farmacológico , Adolescente , Adulto , Egito/epidemiologia , Fasciolíase/epidemiologia , Fasciolíase/parasitologia , Fasciolíase/transmissão , Fezes/parasitologia , Feminino , Seguimentos , Humanos , Masculino , Contagem de Ovos de Parasitas/métodos , Resinas Vegetais , Saúde da População Rural/estatística & dados numéricos , Esquistossomose/epidemiologia , Esquistossomose/parasitologia , Esquistossomose/transmissão , Falha de Tratamento
11.
Indian J Med Microbiol ; 23(2): 95-101, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15928437

RESUMO

PURPOSE: To address the epidemiological characteristics and clinical indices that may predict the prognostic profile of meningitis among children. METHODS: Children admitted to Alexandria fever hospital with clinical diagnosis of meningitis/meningoencephalitis during the period 2002-2003 were recruited for the study. They were subjected to clinical examination as well as CSF bacteriological and serological investigations. RESULTS: Three hundred and ten patients (195 males and 115 females) were included. About 65.2% of them were infected with acute bacterial meningitis (ABM) and 34.8% were infected with aseptic meningitis. In this study, ABM was caused by Haemophilus influenzae (21%), Streptococcus pneumoniae (13.9%), Neisseria meningitidis (14.2%) and other undetermined bacteria (16.1%). ABM showed significant association with age group 1-9 years (66.3%), low socio-economic class (96%), working mother (83.2%), more than two smokers in the family (62.9%) and cold seasons (fall 35.1% and winter 48.5%). Aseptic meningitis showed significant association with age group 3-15 months (100%) and previous immunization (81.5%). The overall case fatality rate was 10.3%; 13.9% for ABM and 3.4% for aseptic meningitis. 7.1% of all survivors developed epileptic attacks. Predictors for death or epilepsy events were high WHO meningitis score (>or=9), decreased CSF glucose level (<10 mg/dL), more smokers in the family, generalised seizures, infancy (<1 year of age) and working mothers. CONCLUSION: This study highlights the importance of several predictors of the outcome of meningitis in children. It is concluded that quick and simple scoring scales, such as the WHO scale, are not only applicable but valuable prognostic tools for meningitis in children.


Assuntos
Haemophilus influenzae/isolamento & purificação , Meningites Bacterianas/diagnóstico , Meningites Bacterianas/epidemiologia , Doença Aguda , Adolescente , Fatores Etários , Biomarcadores/líquido cefalorraquidiano , Criança , Pré-Escolar , Egito , Epilepsia/etiologia , Feminino , Glucose/líquido cefalorraquidiano , Hospitais Urbanos , Humanos , Lactente , Masculino , Meningites Bacterianas/complicações , Meningites Bacterianas/microbiologia , Neisseria meningitidis/isolamento & purificação , Prognóstico , Estudos Prospectivos , Fatores de Risco , Estações do Ano , Classe Social , Streptococcus pneumoniae/isolamento & purificação , Mulheres Trabalhadoras
12.
East Mediterr Health J ; 11(3): 300-7, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-16602448

RESUMO

In January 2002, a pilot programme of neonatal screening for sickle cell disease was launched in the United Arab Emirates (UAE) in 3 districts of Abu Dhabi emirate. This paper reports the incidence of sickle cell diseases, other haemoglobinopathies and haemoglobinopathy carriers over a 12-month period using high performance liquid chromatography as a primary screening method. The overall incidence of sickle cell disease among 22 200 screened neonates was 0.04% (0.07% for UAE citizens and 0.02% for non-UAE citizens). The incidence of sickle cell trait was 1.1% overall (1.5% for UAE citizens and 0.8% for non-UAE citizens). Universal neonatal screening for sickle cell haemoglobin at the national level should be considered.


Assuntos
Anemia Falciforme , Testes Genéticos , Hemoglobinopatias , Triagem Neonatal , Anemia Falciforme/diagnóstico , Anemia Falciforme/epidemiologia , Anemia Falciforme/genética , Coeficiente de Natalidade , Cromatografia Líquida de Alta Pressão , Seguimentos , Aconselhamento Genético , Testes Genéticos/métodos , Testes Genéticos/estatística & dados numéricos , Necessidades e Demandas de Serviços de Saúde , Doença da Hemoglobina C/diagnóstico , Doença da Hemoglobina C/epidemiologia , Doença da Hemoglobina C/genética , Hemoglobina E , Hemoglobinopatias/diagnóstico , Hemoglobinopatias/epidemiologia , Hemoglobinopatias/genética , Hemoglobinas Anormais , Heterozigoto , Humanos , Incidência , Recém-Nascido , Triagem Neonatal/métodos , Triagem Neonatal/estatística & dados numéricos , Projetos Piloto , Vigilância da População , Diagnóstico Pré-Natal , Características de Residência/estatística & dados numéricos , Fatores de Risco , Traço Falciforme/diagnóstico , Traço Falciforme/epidemiologia , Traço Falciforme/genética , Talassemia/diagnóstico , Talassemia/epidemiologia , Talassemia/genética , Emirados Árabes Unidos/epidemiologia
13.
Phys Chem Chem Phys ; 7(11): 2333-9, 2005 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-19785118

RESUMO

The present paper deals with the electroreduction of TaF5 in the room temperature ionic liquid 1-buty-1-methyl-pyrrolidinium bis(tri-fluoromethylsulfonyl)imide ([BMP]Tf2N) at different temperatures for the sake of electrodeposition of tantalum. The study was carried out using cyclic voltammetry and chronoamperometry measurements complemented by SEM-EDAX and XRD investigations. In situ scanning tunneling microscopy and I-U tunneling spectroscopy were also utilized for characterization of the electrodeposits. The results show that, in addition to the formation of insoluble compounds, Ta can be electrodeposited in the ionic liquid ([BMP]Tf2N) containing 0.5 M TaF5 at 200 degrees C on polycrystalline Pt and Au(111) electrodes. By addition of LiF to the electrolyte, the quality and the adherence of the electrodeposit were found to be improved. An in situ I-U tunneling spectrum with about 300 nm thickness of the electrodeposit shows metallic behaviour indicating the formation of elemental tantalum. Moreover, the XRD patterns of the electrodeposit, obtained potentiostatically at -1.8 V (vs. Pt) in ([BMP]Tf2N) containing 0.25 M TaF5 and 0.25 M LiF on Pt electrode at 200 degrees C, show the characteristic patterns of crystalline tantalum.

14.
East Mediterr Health J ; 11(4): 690-9, 2005 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16700385

RESUMO

The National Congenital Anomalies Register is a population-based register covering all births in the United Arab Emirates. We evaluated the progress of the register and determined the prevalence of congenital anomalies (CAs) and associated maternal and neonatal risk factors. Total prevalence of CAs for 1999-2001 was 7.89/1000, 10.95/1000 and 7.92/1000 for live births, stillbirths and total births respectively. Rates were comparable to international rates for all districts except Dubai, Fujairah and Ras Al Khaimah. According to the International classification of diseases, the cardiovascular system was the most affected followed by CAs of chromosomal origin and the musculo-skeletal system. Birth defects were more common with older maternal age, grand multiparity, male babies, low-birth-weight babies and premature babies.


Assuntos
Anormalidades Congênitas/epidemiologia , Vigilância da População , Sistema de Registros , Distribuição por Idade , Coeficiente de Natalidade , Peso ao Nascer , Aberrações Cromossômicas/estatística & dados numéricos , Anormalidades Congênitas/classificação , Anormalidades Congênitas/etiologia , Anormalidades Congênitas/prevenção & controle , Consanguinidade , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Classificação Internacional de Doenças , Masculino , Idade Materna , Paridade , Vigilância da População/métodos , Gravidez , Resultado da Gravidez/epidemiologia , Prevalência , Prevenção Primária , Características de Residência , Fatores de Risco , Distribuição por Sexo , Natimorto/epidemiologia , Emirados Árabes Unidos/epidemiologia
15.
Vet Parasitol ; 103(4): 323-32, 2002 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-11777611

RESUMO

Reports on the species of Fasciola present in the Nile Delta, Egypt, appear controversial. Some authors reported the presence of both Fasciola gigantica and Fasciola hepatica, others reported F. gigantica only and mentioned that F. hepatica was found only in imported animals. This study was an attempt to identify the species of Fasciola flukes collected from locally bred animals. Morphologic, morphoanatomic, morphometric, and chemotaxonomic criteria of the fluke isolates were studied. Speciation based on morphologic and morphometric data was not decisive due to overlap in the values of most measurements. Morphoanatomic data proved the presence of both the species, and isoelectric focusing (IEF) of fluke soluble protein confirmed the presence of both F. gigantica and F. hepatica in Egypt.


Assuntos
Búfalos/parasitologia , Doenças dos Bovinos/parasitologia , Fasciola/isolamento & purificação , Fasciolíase/veterinária , Proteínas de Helminto/análise , Animais , Bovinos , Egito , Fasciola/química , Fasciola/classificação , Fasciola hepatica , Fasciolíase/parasitologia , Focalização Isoelétrica/veterinária , Filogenia
16.
East Mediterr Health J ; 8(4-5): 619-25, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-15603045

RESUMO

Intra-specimen and day-to-day variations of Fasciola egg counts in stools were investigated for 16 cases of established fascioliasis. For each case six Kato slides from a single stool sample were examined daily for 5 consecutive days. The results indicated the presence of significant intra-specimen variations in more than one-third of the examined series, while the inter-specimen variation was almost negligible. The sensitivity of the Kato-Katz test for diagnosing Fasciola infection with three Kato slides from the same specimen or on different days ranged from 96.0%-99.1%. The examination of three Kato smears from a single stool specimen, which is more feasible in field studies, would give an accurate diagnosis of fascioliasis. Used as such, the Kato-Katz technique is highly sensitive in the diagnosis of fascioliasis.


Assuntos
Fasciola , Fasciolíase/diagnóstico , Fezes/parasitologia , Contagem de Ovos de Parasitas/métodos , Adulto , Animais , Viés , Criança , Egito/epidemiologia , Fasciola/fisiologia , Fasciolíase/epidemiologia , Fasciolíase/parasitologia , Estudos de Viabilidade , Humanos , Contagem de Ovos de Parasitas/normas , Prevalência , Sensibilidade e Especificidade , Especificidade da Espécie , Fatores de Tempo
17.
J Pharm Pharmacol ; 53(6): 889-94, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11428666

RESUMO

A novel tryptamine analog, 1-methyl-3-[N-(3-indolyl)ethyl]carbamoyl-1,4-dihydropyridine (T-CDS) was synthesized and converted into a stable, solid complex with 2-hydroxypropyl-beta-cyclodextrin. An aqueous solution of the complex was given intravenously to dogs and the concentration of T-CDS and its corresponding quaternary (T-Q+) forms were monitored in the blood for 50 min. The effect of the drug on vital heart parameters was monitored throughout the studies. At the end of the experiment the dogs were sacrificed and the concentration of the quaternary pyridinium form (T-Q+) was determined in the different heart tissues, as well as in the kidney, liver, lung, brain, urine and cerebrospinal fluid. The compound was found to be selectively bound to the heart muscles and showed different concentrations in different heart tissues. The T-Q+ concentrations were much higher in the heart after administration of the dihydro form (T-CDS), than after administering T-Q+ directly. The compound was found to be active on certain vital signs of the cardiovascular system and could be an effective and safe antiarrhythmic agent.


Assuntos
Antiarrítmicos/farmacologia , Coração/efeitos dos fármacos , Coração/fisiologia , Miocárdio/química , Triptaminas/farmacologia , Animais , Sítios de Ligação , Cães , Feminino , Infusões Intravenosas , Masculino , Distribuição Tecidual , Triptaminas/síntese química , Triptaminas/farmacocinética
18.
AAPS PharmSci ; 3(4): E30, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-12049493

RESUMO

Receptor binding studies were performed on 24 soft anticholinergic agents and 5 conventional anticholinergic agents using 4 cloned human muscarinic receptor subtypes. The measured pK(i) values of the soft anticholinergic agents ranged from 6.5 to 9.5, with the majority being in the range of 7.5 to 8.5. Strong correlation was observed between the pK(i) s determined here and the pA 2 values measured earlier in guinea pig ileum contraction assays. The corresponding correlation coefficients (r2) were 0.80, 0.73, 0.81, and 0.78 for pK(i) (m1), pK(i) (m2), pK(i) (m3), and pK(i) (m4), respectively. Quantitative structure-activity relationship (QSAR) studies were also performed, and good characterization could be obtained for the soft anticholinergics containing at least 1 tropine moiety in their structure. For these compounds, the potency as measured by the pK i values was found to be related to geometric, electronic, and lipophilicity descriptors. A linear regression equation using ovality (O(e)), dipole moment (D), and a calculated log octanol-water partition coefficient (QLogP) gave reasonably good descriptions (r = 0.88) for the pK(i) (m3) values.


Assuntos
Antagonistas Colinérgicos/metabolismo , Receptores Muscarínicos/metabolismo , Animais , Antagonistas Colinérgicos/síntese química , Relação Dose-Resposta a Droga , Estabilidade de Medicamentos , Cobaias , Humanos , Íleo/química , Ligação Proteica , Relação Quantitativa Estrutura-Atividade
19.
East Mediterr Health J ; 6(5-6): 919-25, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-12197349

RESUMO

The detection of IgG avidity in sera is potentially useful in the diagnosis of acute and chronic infection. We studied IgG avidity in 31 patients with fascioliasis, with the aim of evaluating the clinical application of this test to confirm the diagnosis of incubating cases and to distinguish between acute and chronic cases. Of the 31 cases, 13 were incubating and had a mean avidity index of 57.28 +/- 5.79%. The 18 chronic cases had an avidity index of 68.80 +/- 8.92%. The difference was highly significant. We conclude that IgG avidity is a reliable means of identifying the stage of fascioliasis and suggest a cut-off point of 59.90% to distinguish between acute and chronic infection.


Assuntos
Anticorpos Anti-Helmínticos , Afinidade de Anticorpos/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Fasciola/imunologia , Fasciolíase/diagnóstico , Imunoglobulina G , Adolescente , Adulto , Animais , Anticorpos Anti-Helmínticos/imunologia , Ensaio de Imunoadsorção Enzimática/normas , Fasciolíase/classificação , Fasciolíase/imunologia , Fasciolíase/parasitologia , Testes de Hemaglutinação/normas , Humanos , Imunoglobulina G/imunologia , Programas de Rastreamento/métodos , Programas de Rastreamento/normas , Pessoa de Meia-Idade , Contagem de Ovos de Parasitas , Sensibilidade e Especificidade , Índice de Gravidade de Doença , Fatores de Tempo
20.
Trop Med Int Health ; 4(10): 686-90, 1999 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10583902

RESUMO

An IELISA was developed to evaluate the performance of Fasciola E/S antigens in diagnosis and cure assessment of human Fasciola infection. Twenty patients with acute (prepatent) fascioliasis and another 20 with patent infection were enrolled in the study. Patients were treated with TCZ and followed at 1, 3 and 6 months after therapy. At inspection, the sensitivity of the test to diagnose prepatent cases was 100% compared to 70% for patent infections. There was a gradual decrease of antigenaemia over the follow-up period in acute cases. In chronic cases antigen disappeared from 13 cases (65%) at 1 month; this proportion did not change at 3 or 6 months.


Assuntos
Antígenos de Helmintos/análise , Ensaio de Imunoadsorção Enzimática/métodos , Fasciolíase/diagnóstico , Reação de Fase Aguda , Animais , Anti-Helmínticos/uso terapêutico , Antígenos de Helmintos/sangue , Benzimidazóis/uso terapêutico , Doença Crônica , Fasciola/isolamento & purificação , Fasciolíase/tratamento farmacológico , Fasciolíase/imunologia , Seguimentos , Humanos , Testes Sorológicos , Triclabendazol
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