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1.
Braz. j. biol ; 82: e232805, 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153457

RESUMO

Abstract One of the biological indicators most used to determine the health of a fluvial ecosystem are the benthic macroinvertebrates. The presence of recurrent species in a wide gradient of latitudes, dominates the biogeographic pattern of the benthic macroinvertebrates in Chilean fresh waters, nevertheless the knowledge on the communitarian ecology of these in the Chilean rivers continues to be scarce. Null models became a powerful statistical tool for describing the ecological mechanisms that drive the structure of an ecological community and the underlying patterns of diversity. The objective of this study was to determine the community structure of benthic invertebrates in the Allipén River by describing their composition, richness and abundance of species through richness models and null models based on presence/absence. The results reveal a high family richness and low diversity, three phyla, five classes, 11 orders and 28 families were identified in the study area during the four seasons of the year. The Arthropoda phylum was the most representative in abundance and richness. Regarding to richness, Trichoptera (7 families) and Diptera (6 families) followed by Ephemeroptera (3 families) were the orders that showed the greatest diversity of families, however, a low diversity with a H'≤ 1.5 nit was registered in the study area. We demonstrated through the null models, the randomization in the species associations corresponding to the three analyzed sites. The information provided here contributes to the understanding of the ecological patterns of the invertebrate communities in the Allipén River, establishing the basis for more complex ecological studies.


Resumo Um dos indicadores biológicos mais utilizados para determinar a saúde de um ecossistema fluvial são os macroinvertebrados bentônicos. A presença de espécies recorrentes em um amplo gradiente de latitudes domina o padrão biogeográfico dos macroinvertebrados bentônicos nas águas doces do Chile; no entanto, o conhecimento sobre a ecologia comunitária destes nos rios chilenos continua escasso. Os modelos nulos se tornaram uma poderosa ferramenta estatística para descrever os mecanismos ecológicos que orientam a estrutura de uma comunidade ecológica e os padrões subjacentes da diversidade. O objetivo deste estudo foi determinar a estrutura da comunidade de invertebrados bentônicos no rio Allipén, descrevendo sua composição, riqueza e abundância de espécies através de modelos de riqueza e modelos nulos baseados na presença / ausência. Os resultados revelam alta riqueza de espécies e baixa diversidade, sendo identificados três filos, cinco classes, 11 ordens e 28 famílias na área de estudo durante as quatro estações do ano. O filo de Arthropoda foi o mais representativo em abundância e riqueza. Em relação à riqueza, Trichoptera (7) e Diptera (6) seguidos por Ephemeroptera (3) foram as ordens que mostraram a maior diversidade de famílias, no entanto, uma baixa diversidade com H'≤ 1,5 nit foi registrada na área de estudo. Demonstramos através dos modelos nulos, a randomização nas associações de espécies correspondentes aos três locais analisados. As informações aqui fornecidas contribuem para a compreensão dos padrões ecológicos das comunidades de invertebrados no rio Allipén, estabelecendo a base para estudos ecológicos mais complexos.

2.
Arch Environ Contam Toxicol ; 80(3): 624-633, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33740087

RESUMO

The bioaccumulation of arsenic (As) in the muscle, liver, kidneys, and brain of the shark Sphyrna lewini was measured in 40 juvenile specimens from southeast Gulf of California. Additionally, the biomagnification factor was calculated through prey items from stomach contents of the analyzed specimens. The concentrations of As (mg kg-1, wet weight) were higher in the muscle (10.1 ± 0.3) and liver (9.4 ± 0.5) than in the brain (4.5 ± 0.3) and kidneys (4.2 ± 0.2), which may be attributed to the biological functions of each tissue. Positive correlations were found between the levels of As in muscle and liver with the biological parameters of S. lewini. Hammerhead sharks feed mainly of teleost fishes with low As values (Clupeidae fishes, 1.1 ± 0.5; Sciaenidae fishes, 1.0 ± 0.6; Scomber japonicus, 1.2 ± 0.6; and Etropus crossotus 2.1 ± 0.4) compared with the predator, indicating biomagnification. Inorganic arsenic (Asi) in muscle was estimated as 3% of the total As, although muscle consumption is unlikely to represent a risk (HQ < 1) in humans. Moreover, the probabilities of developing cancer were estimated as low (3.99 × 10-5 to 3.32 × 10-6). To avoid health risks related to As, a weekly ration must not exceed 69.3 and 484.8 g in children and adults, respectively.


Assuntos
Arsênico , Tubarões , Animais , California , Criança , Peixes , Humanos , Alimentos Marinhos
3.
Braz J Biol ; 82: e232805, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33787712

RESUMO

One of the biological indicators most used to determine the health of a fluvial ecosystem are the benthic macroinvertebrates. The presence of recurrent species in a wide gradient of latitudes, dominates the biogeographic pattern of the benthic macroinvertebrates in Chilean fresh waters, nevertheless the knowledge on the communitarian ecology of these in the Chilean rivers continues to be scarce. Null models became a powerful statistical tool for describing the ecological mechanisms that drive the structure of an ecological community and the underlying patterns of diversity. The objective of this study was to determine the community structure of benthic invertebrates in the Allipén River by describing their composition, richness and abundance of species through richness models and null models based on presence/absence. The results reveal a high family richness and low diversity, three phyla, five classes, 11 orders and 28 families were identified in the study area during the four seasons of the year. The Arthropoda phylum was the most representative in abundance and richness. Regarding to richness, Trichoptera (7 families) and Diptera (6 families) followed by Ephemeroptera (3 families) were the orders that showed the greatest diversity of families, however, a low diversity with a H'≤ 1.5 nit was registered in the study area. We demonstrated through the null models, the randomization in the species associations corresponding to the three analyzed sites. The information provided here contributes to the understanding of the ecological patterns of the invertebrate communities in the Allipén River, establishing the basis for more complex ecological studies.


Assuntos
Ecossistema , Rios , Animais , Chile , Monitoramento Ambiental , Água Doce , Humanos , Invertebrados
4.
Mar Pollut Bull ; 164: 112102, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33561583

RESUMO

The purpose of this work was to use the deep-water chimaera Hydrolagus colliei to examine the bioaccumulation and availability of Hg and Se in its deep-water habitat; the Se:Hg molar ratio was calculated to establish baseline information of the species and its associated ecosystem. The organisms were collected from northern Gulf of California. Hg levels in muscle were higher than liver and the opposite pattern happened with Se concentrations. Female had total lengths and weights higher than male but there were not found differences between elements concentrations by sex. Hg in muscle was correlated with weight. Molar Se:Hg ratio in muscle was the lowest compared to the liver. It was hypothesized that Hg and Se uptake to H. colliei were by its feeding habits because is a dominant species component of the demersal ecosystem and that play and important functional role in the control of oceanic ecosystem structure and function.


Assuntos
Mercúrio , Selênio , Poluentes Químicos da Água , Animais , California , Ecossistema , Monitoramento Ambiental , Feminino , Masculino , Mercúrio/análise , Oceanos e Mares , Selênio/análise , Poluentes Químicos da Água/análise
5.
Artigo em Inglês | MEDLINE | ID: mdl-33558975

RESUMO

Distribution of arsenic (As) in tissues and gonads of the Indo-Pacific sailfish Istiophorus platypterus and the dolphinfish Coryphaena hippurus from the SE Gulf of California was evaluated. The bioaccumulation patterns of As were the same in the two species. In I. platypterus, As levels (mg kg-1, wet weight) were gonads (7.4 ± 1.1) > liver (3.1 ± 0.1) > kidney (2.7 ± 0.1) > muscle (1.6 ± 0.1); in C. hippurus, As (mg kg-1) levels were gonads (4.3 ± 0.6) > liver (3.2 ± 0.2) > kidney (2.3 ± 0.1) > muscle (1.2 ± 0.1). Differences in As distribution could be attributed to the biological functions of tissues. The hypothesis was confirmed that biomagnification was evidenced by the fact that As levels were lower in prey species than in predators. Intake of muscle from either fish did not represent a risk to humans if recommended portions a week are not exceeded, adults as much as 1802.4 g and 2454.1 g and children 257.5 and 350.6 g, for sailfish and dolphinfish, respectively. In addition, the likelihood of developing cancer due to consumption of edible tissues from either of these top predators was in the acceptable range (6.4 × 10-5 to 27.3 × 10-6 for a population that consumes 50 g of muscle in a week) but if a conservative combined slope factor is used the probabilities to develop bladder and lung cancer increments from 1.1 × 10-3 to 9.1 × 10-5.

6.
Rev. chil. obstet. ginecol. (En línea) ; 85(supl.1): S35-S49, set. 2020. graf
Artigo em Espanhol | LILACS | ID: biblio-1138647

RESUMO

INTRODUCCIÓN: Ante el escenario de pacientes obstétricas diagnosticadas por la nueva enfermedad por coronavirus (COVID-19), se hace imprescindible la necesidad de realizar un seguimiento adecuado tanto desde el punto de vista de obstétrico como del cuadro viral, durante el período de aislamiento domiciliario. Con este objetivo, se desarrolló un programa de seguimiento remoto por la Unidad de Medicina Materno Fetal (MMF) de nuestro centro, desde el diagnóstico de la infección hasta el alta médica según los criterios vigentes del Ministerio de Salud (MINSAL). OBJETIVO: Evaluar la satisfacción del paciente en el seguimiento remoto de embarazadas y puérperas con diagnóstico de infección por SARS-CoV-2. Material y Métodos: Estudio descriptivo mediante la aplicación de una encuesta anónima y voluntaria de satisfacción usuaria, a través de una plataforma online. El instrumento fue una adaptación de la versión en español del "Telehealth Usability Questionnaire" (TUQ) previamente validado. RESULTADOS: Se obtuvo un 94,5% de respuestas a la encuesta. El 86% de las pacientes evaluó como "Muy Bueno" o "Bueno" el control virtual. El 91% no experimentó problemas técnicos. El 14% de las pacientes prefiere un control virtual, el 26% presencial y el 60% un control mixto. El 80% cumplió todas sus necesidades con el control virtual. CONCLUSIONES: En el contexto de pandemia e infección por SARS-CoV-2, el seguimiento remoto ha sido evaluado globalmente en forma positiva con niveles aceptables de satisfacción de las pacientes.


INTRODUCTION: Given the scenario of obstetric patients diagnosed with the new coronavirus disease (COVID-19), the need for proper follow-up both obstetric and of the infection, during the period of home isolation, is essential. A remote monitoring program was developed by the Maternal Fetal Medicine Unit (MFM) of our center from the diagnosis of the infection until medical discharge according to the current criteria of the Ministry of Health (MINSAL). OBJECTIVE: To assess patient satisfaction of remote monitoring of pregnant and postpartum patients diagnosed with SARS-CoV-2 infection. METHODS: This is a descriptive-survey research. We performed a patient satisfaction survey through an online platform to pregnant and postpartum patients with remote monitoring for SARS-CoV-2 infection in our center. The survey was anonymous and voluntary. The instrument for this purpose was the Spanish adapted version of "Telehealth Usability Questionnaire" (TUQ) that has been previously validated. RESULTS: The survey was answered by 94,5% of the patients. The results show that 86% of the patients rated the virtual control as "Very Good" or "Good"; 91% did not experienced technical problems. 14% of the patients prefer virtual control, 26% prefer face-to-face control and 60% both virtual and face-to-face control. 80% fulfilled all their needs with virtual control. CONCLUSIONS: In the context of pandemic and SARS-CoV-2 infection, remote monitoring has been positively evaluated with acceptable levels of patient's satisfaction.


Assuntos
Humanos , Feminino , Gravidez , Pneumonia Viral/psicologia , Pneumonia Viral/terapia , Complicações Infecciosas na Gravidez/psicologia , Complicações Infecciosas na Gravidez/terapia , Infecções por Coronavirus/psicologia , Infecções por Coronavirus/terapia , Período Pós-Parto , Epidemiologia Descritiva , Inquéritos e Questionários , Seguimentos , Satisfação do Paciente , Telemedicina/métodos , Pandemias , Betacoronavirus , Realidade Virtual
7.
Br J Oral Maxillofac Surg ; 57(7): 632-637, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31200980

RESUMO

This epidemiological study was designed to find out the incidence and factors associated with the occurrence of intraosseous lesions diagnosed at a Reference Centre in Brazil. We included all patients diagnosed with intraosseous lesions (cyst, tumour, bone-associated lesion, and periapical disease) during the period 2006-2017, and analysed the association between some sociodemographic and clinical variables and the types of lesion. There was a total of 290 intraosseous lesions, the most common being odontogenic cysts. There was a significant association between age and odontogenic tumours (p=0.001). In relation to the histopathological diagnosis, root cysts were the most common (n=57), followed by dentigerous cysts (n=26). The lesions studied were seen most often in women between the second and fourth decades of life, odontogenic cysts being the most common type found. We know of few publications of similar epidemiological work, either in Brazil or in the rest of the world, so we suggest that more such studies are made.


Assuntos
Cisto Dentígero/patologia , Cistos Odontogênicos/epidemiologia , Tumores Odontogênicos/epidemiologia , Doenças Estomatognáticas/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Brasil/epidemiologia , Criança , Pré-Escolar , Cisto Dentígero/epidemiologia , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Cistos Odontogênicos/patologia , Tumores Odontogênicos/patologia , Estudos Retrospectivos , Doenças Estomatognáticas/patologia , Adulto Jovem
8.
Mar Pollut Bull ; 138: 84-92, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30660317

RESUMO

Selenium and mercury were evaluated in tissues and stomachs of the dolphinfish Coryphaena hippurus from two sites in the Southeastern Gulf of California. Hg levels were consistently low and exhibited the following patterns: muscle (0.14 ±â€¯0.01 µg/g wet weight) followed by kidney (0.12 ±â€¯0.02 µg/g), liver (0.11 ±â€¯0.01 µg/g), and gonads (0.04 ±â€¯0.00 µg/g). The maximum of Se was found in the kidney (5.60 ±â€¯0.40 µg/g) and the minimum in muscle (0.60 ±â€¯0.01 µg/g). All the Se:Hg molar ratios were between 3.7 and 697.1, with the minimum in muscle and the highest in gonads. The results indicate a contrasting behavior biomagnification of Hg and Se; smaller fish (<80 cm FL) did not exhibit biomagnification, in contrast with larger fish (>90 cm FL: 100% for Hg; 65% for Se). These results appear to be related to different feeding habits and availability of prey.


Assuntos
Conteúdo Gastrointestinal/química , Mercúrio/análise , Perciformes , Selênio/análise , Poluentes Químicos da Água/análise , Animais , Exposição Dietética/efeitos adversos , Feminino , Produtos Pesqueiros/análise , Contaminação de Alimentos/análise , Humanos , Rim/química , Masculino , Mercúrio/farmacocinética , México , Músculos/química , Oceano Pacífico , Medição de Risco , Selênio/farmacocinética , Distribuição Tecidual , Poluentes Químicos da Água/farmacocinética
9.
Arch. endocrinol. metab. (Online) ; 62(6): 623-635, Dec. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-983814

RESUMO

ABSTRACT Objective: Initial diagnosis of medullary thyroid carcinoma (MTC) is frequently associated with advanced stages and a poor prognosis. Thus, the need for earlier diagnoses and detection in relatives at risk for the disease has led to increased use of RET genetic screening. Subjects and methods: We performed RET screening in 247 subjects who were referred to the Brazilian Research Consortium for Multiple Endocrine Neoplasia (BRASMEN) Center in the State of Ceará. Direct genetic sequencing was used to analyze exons 8, 10, 11, and 13-16 in MTC index cases and specific exons in at risk relatives. Afterward, clinical follow-up was offered to all the patients with MTC and their affected relatives. Results: RET screening was performed in 60 MTC index patients and 187 at-risk family members. At the initial clinical assessment of the index patients, 54 (90%) were diagnosed with apparently sporadic disease and 6 (10%) diagnosed with hereditary disease. After RET screening, we found that 31 (52%) index patients had sporadic disease, and 29 (48%) had hereditary disease. Regarding at-risk relatives, 73/187 were mutation carriers. Mutations in RET codon 804 and the rare p.M918V mutation were the most prevalent. Conclusions: Performing RET screening in Ceará allowed us to identify a different mutation profile in this region compared with other areas. RET screening also enabled the diagnosis of a significant number of hereditary MTC patients who were initially classified as sporadic disease patients and benefited their relatives, who were unaware of the risks and the consequences of bearing a RET mutation.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/genética , Mutação em Linhagem Germinativa/genética , Carcinoma Neuroendócrino/diagnóstico , Carcinoma Neuroendócrino/genética , Proteínas Proto-Oncogênicas c-ret/genética , Triagem de Portadores Genéticos/métodos , Fatores de Tempo , Brasil , Neoplasias da Glândula Tireoide/patologia , Imuno-Histoquímica , Transfecção/métodos , Rearranjo Gênico/genética , Reprodutibilidade dos Testes , Fatores de Risco , Fatores Etários , Carcinoma Neuroendócrino/patologia , Medição de Risco , Detecção Precoce de Câncer , Estudos de Associação Genética
10.
Anim Reprod Sci ; 192: 164-170, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29555193

RESUMO

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highest percentage of motility was recorded with salinity 34.6 g L-1 (95 ±â€¯10%) and the longest motility time was obtained with a salinity of 34.8 g L-1 (189 ±â€¯15 s). Variations in the salinity between 30 and 35 g L-1 did not produce any significant alterations in sperm motility; however salinities of 20 and 50 g L-1 produced a significant loss of sperm motility. The highest percentage of motility was obtained at pH 8.5 (93 ±â€¯12%), and the longest motility period at pH 8.7 (218 ±â€¯13 s), while pH lower than or equal to 7 and equal to 10 both produced a significant loss in sperm motility. A positive correlation was found between pH/salinity and the motility percentage (R2 = 0.94 and R2 = 0.97) and motility time (R2 = 0.86 and R2 = 0.98). In seminal and morphometric parameters, statistically significant differences were observed in semen volume, sperm density, plasma membrane integrity and sperm morphometry between the groups studied, showing that the characteristics of the fish have a direct influence on sperm quality. The information generated in this research will be useful for developing biotechnology tools for the effective management of Mugil liza gametes.


Assuntos
Peixes/fisiologia , Salinidade , Espermatozoides/fisiologia , Animais , Animais Selvagens , Aquicultura , Concentração de Íons de Hidrogênio , Masculino , Tolerância ao Sal , Motilidade Espermática/efeitos dos fármacos
11.
Andrologia ; 50(2)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28730739

RESUMO

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus mykiss) in different reproductive ages (2, 3 and 4 years old). Sperm samples of each reproductive age were stored in Storfish® during 10 days at 4°C, and then, motility, viability, mitochondrial function (MMP), superoxide anion (O2-) level and DNA fragmentation (DNAfrag ) were assessed. The results demonstrated that sperm function parameters were affected significantly by the age of the males and the time of storage. Motility, viability and MMP significantly decreased, and DNAfrag and O2- level increased with the age increment and the time of storage. In conclusion, sperm quality of 2 and 3 years old were superior to those of 4 years old, based on higher quality of various sperm functions such as motility, viability, MMP, DNA integrity and level O2- during short-term storage. This information must be considered for optimum utilization of broodstock males in aquaculture.


Assuntos
Envelhecimento/fisiologia , Aquicultura/métodos , Oncorhynchus mykiss/fisiologia , Refrigeração/métodos , Preservação do Sêmen/métodos , Animais , Cruzamento/métodos , Fragmentação do DNA , Masculino , Sêmen/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Superóxidos/metabolismo
12.
Andrologia ; 50(2)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28758699

RESUMO

Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mechanism. We determined the expression levels of mitochondrial and nuclear OGG1 isoforms in spermatozoa collected from cauda epididymis in rats exposed to chronic hypobaric hypoxia (CHH) for 5, 15 and 30 days. CHH attenuates OGG1 expression in a time-dependent fashion, with a greater reduction in the mitochondrial isoform OGG1-2a (p < .05). Attenuation of the BER system may contribute to DNA damage under hypoxia exposure.


Assuntos
Dano ao DNA/fisiologia , DNA Glicosilases/metabolismo , Reparo do DNA/fisiologia , Estresse Oxidativo/fisiologia , Espermatozoides/metabolismo , Animais , Hipóxia Celular/fisiologia , Núcleo Celular/metabolismo , Isoenzimas/metabolismo , Masculino , Mitocôndrias/metabolismo , Modelos Animais , Ratos , Espermatozoides/citologia
13.
Arch Endocrinol Metab ; 62(6): 623-635, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30624503

RESUMO

OBJECTIVE: Initial diagnosis of medullary thyroid carcinoma (MTC) is frequently associated with advanced stages and a poor prognosis. Thus, the need for earlier diagnoses and detection in relatives at risk for the disease has led to increased use of RET genetic screening. SUBJECTS AND METHODS: We performed RET screening in 247 subjects who were referred to the Brazilian Research Consortium for Multiple Endocrine Neoplasia (BRASMEN) Center in the State of Ceará. Direct genetic sequencing was used to analyze exons 8, 10, 11, and 13-16 in MTC index cases and specific exons in at risk relatives. Afterward, clinical follow-up was offered to all the patients with MTC and their affected relatives. RESULTS: RET screening was performed in 60 MTC index patients and 187 at-risk family members. At the initial clinical assessment of the index patients, 54 (90%) were diagnosed with apparently sporadic disease and 6 (10%) diagnosed with hereditary disease. After RET screening, we found that 31 (52%) index patients had sporadic disease, and 29 (48%) had hereditary disease. Regarding at-risk relatives, 73/187 were mutation carriers. Mutations in RET codon 804 and the rare p.M918V mutation were the most prevalent. CONCLUSIONS: Performing RET screening in Ceará allowed us to identify a different mutation profile in this region compared with other areas. RET screening also enabled the diagnosis of a significant number of hereditary MTC patients who were initially classified as sporadic disease patients and benefited their relatives, who were unaware of the risks and the consequences of bearing a RET mutation.


Assuntos
Carcinoma Neuroendócrino/diagnóstico , Carcinoma Neuroendócrino/genética , Triagem de Portadores Genéticos/métodos , Mutação em Linhagem Germinativa/genética , Proteínas Proto-Oncogênicas c-ret/genética , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/genética , Adolescente , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Brasil , Carcinoma Neuroendócrino/patologia , Detecção Precoce de Câncer , Feminino , Rearranjo Gênico/genética , Estudos de Associação Genética , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Medição de Risco , Fatores de Risco , Neoplasias da Glândula Tireoide/patologia , Fatores de Tempo , Transfecção/métodos , Adulto Jovem
14.
Rehabilitación (Madr., Ed. impr.) ; 51(1): 43-51, ene.-mar. 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-160485

RESUMO

Objetivo. El objetivo de este trabajo es realizar una actualización sobre el tratamiento rehabilitador de la distonía cervical (DC), tanto de su eficacia como de las estrategias utilizadas. Búsqueda y selección de estudios. Se identificaron 21 estudios mediante la búsqueda en bases de datos electrónicas de los artículos científicos publicados tanto en inglés como en castellano entre enero de 2000 y febrero de 2016, con las palabras clave: distonía cervical, tratamiento, rehabilitación, fisioterapia, tortícolis espasmódica. Los criterios de inclusión fueron: ensayos clínicos con pacientes diagnosticados de DC en tratamiento rehabilitador y con una calidad metodológica aceptable según la escala de Jadad. Resultados y conclusiones. Se han seleccionado 5 estudios. En líneas generales se aconseja: ejercicios de relajación, estiramientos, reeducación motora, equilibrio, coordinación y propiocepción. Los ejercicios deben ser inicialmente tutelados y una vez aprendidos deben realizarse en sesiones cortas y frecuentes de autorrehabilitación con el objetivo de inducir neuroplasticidad. Parece claro que la asociación de un programa fisioterápico al tratamiento con toxina botulínica es beneficioso (AU)


Objective. The aim of this study was to provide an update on rehabilitation treatment for cervical dystonia (CD), in terms of both its effectiveness and the strategies used. Study search and selection strategy. A total of 21 studies were identified by a search in electronic database for scientific articles published in English or Spanish from January 2000 to February 2016, with the keywords: cervical dystonia, treatment, rehabilitation, physiotherapy, and spasmodic torticollis. Inclusion criteria were: clinical trials of patients diagnosed with CD receiving rehabilitation treatment and with an adequate methodological quality according to the Jadad scale. Results and conclusions. Five studies were selected. In general, we recommend: relaxation exercises, stretching, motor re-education, balance, coordination and proprioception. The exercises should initially be supervised and, once learned, should be made in short and frequent self-rehabilitation sessions in order to induce neuroplasticity. It seems clear that the combination of a physiotherapeutic program and botulinum toxin treatment is beneficial (AU)


Assuntos
Humanos , Masculino , Feminino , Torcicolo/reabilitação , Torcicolo/terapia , Modalidades de Fisioterapia , Toxinas Botulínicas Tipo A/uso terapêutico , Relaxamento Muscular/fisiologia , Exercícios de Alongamento Muscular/métodos , Resultado do Tratamento , Transtornos das Habilidades Motoras/reabilitação , Propriocepção/fisiologia , Eletromiografia
15.
Andrologia ; 49(5)2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27506323

RESUMO

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our study was to analyse the effect of a sodium alginate-based extender on sperm function in the short-term storage of salmonids semen. Samples of Salmo salar, Oncorhynchus kisutch, and Oncorhynchus mykiss were stored in Storfish® (Ext-C) and Storfish® supplemented with sodium alginate (Ext-A) during 10 days at 4°C. After storage, motility, viability, mitochondrial membrane potential (ΔΨmit), superoxide anion (O2- ) level and DNA fragmentation (DNA Frag) were assessed. Ext-A had positive effect in preservation of sperm motility, viability, ΔΨmit, O2- level and DNA integrity in the three species analysed compared to control samples. In Ext-A, the spermatozoa of S. salar and O. mykiss showed significantly higher motility, viability and ΔΨmit than O. kisutch. However, O. kisutch and O. mykiss had significantly lower O2- level than S. salar, and DNA fragmentation in O. kisutch and S. salar was significantly lower than in samples of O. mykiss (p < 0.05). Dilution of salmonids semen in a sodium alginate-based extender is effective for protecting sperm quality during 10 days of short-term storage.


Assuntos
Alginatos , Salmonidae , Preservação do Sêmen/veterinária , Animais , Sobrevivência Celular , Fragmentação do DNA , Ácido Glucurônico , Ácidos Hexurônicos , Masculino , Potencial da Membrana Mitocondrial , Preservação do Sêmen/métodos , Motilidade Espermática , Superóxidos/análise , Fatores de Tempo
16.
J Fish Biol ; 89(3): 1537-50, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27406003

RESUMO

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium + 1.3M dimethyl sulphoxide + 0.3M glucose + 2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as the treatment (T) and fresh semen as the control (F). Straws of 0·5 ml of sperm suspension were frozen in 4 cm of N2 L. They were thawed in a thermoregulated bath (40° C). After thawing, the percentage of spermatozoa with fragmented DNA [transferase dUTP (deoxyuridine triphosphate) nick-end labelling (TUNEL)], plasma membrane integrity (SYBR-14/PI) and mitochondrial membrane potential (ΔΨMMit, JC-1) were evaluated by flow cytometry and motility was evaluated by optical microscope under stroboscopic light. The fertilization rates of the control and treatment semen were tested at a sperm density of 1·5 × 10(7) spermatozoa oocyte(-1) , by observation of the first cleavages after 16 h incubation at 10° C. In the cryopreserved semen (T), the mean ± s.d. DNA fragmentation was 4·8 ± 2·5%; plasma membrane integrity 75·2 ± 6·3%; mitochondrial membrane potential 51·7 ± 3·6%; motility 58·5 ± 5·3%; curved line velocity (VCL ) 61·2 ± 17·4 µm s(-1) ; average-path velocity (VAP ) 50·1 ± 17·3 µm s(-1) ; straight-line velocity (VSL ) 59·1 ± 18·4 µm s(-1) ; fertilization rate 81·6 ± 1·9%. There were significant differences in the plasma membrane integrity, mitochondrial membrane potential, motility, fertilization rate, VCL , VAP and VSL compared with the controls (P < 0·05). Also the mitochondrial membrane potential correlated with motility, fertilization rate, VCL and VSL (r = 0·75; r = 0·59; r = 0·77 and r = 0·79, respectively; P < 0·05); and the fertilization rate correlated with VCL and VSL (r = 0·59 and r = 0·55, respectively).


Assuntos
Criopreservação , Salmo salar , Preservação do Sêmen , Animais , Crioprotetores , Fertilização , Masculino , Microscopia Confocal , Oócitos , Compostos Orgânicos , Sêmen , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/fisiologia
17.
Curr Opin Insect Sci ; 15: 131-8, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27436743

RESUMO

Transgenic crops producing Bacillus thuringiensis- (Bt) insecticidal proteins (Bt crops) have provided useful pest management tools to growers for the past 20 years. Planting Bt crops has reduced the use of synthetic insecticides on cotton, maize and soybean fields in 11 countries throughout Latin America. One of the threats that could jeopardize the sustainability of Bt crops is the development of resistance by targeted pests. Governments of many countries require vigilance in measuring changes in Bt-susceptibility in order to proactively implement corrective measures before Bt-resistance is widespread, thus prolonging the usefulness of Bt crops. A pragmatic approach to obtain information on the effectiveness of Bt-crops is directly asking growers, crop consultants and academics about Bt-resistance problems in agricultural fields, first-hand information that not necessarily relies on susceptibility screens performed in laboratories. This type of information is presented in this report. Problematic pests of cotton and soybeans in five Latin American countries currently are effectively controlled by Bt crops. Growers that plant conventional (non-Bt) cotton or soybeans have to spray synthetic insecticides against multiple pests that otherwise are controlled by these Bt crops. A similar situation has been observed in six Latin American countries where Bt maize is planted. No synthetic insecticide applications are used to control corn pests because they are controlled by Bt maize, with the exception of Spodoptera frugiperda. While this insect in some countries is still effectively controlled by Bt maize, in others resistance has evolved and necessitates supplemental insecticide applications and/or the use of Bt maize cultivars that express multiple Bt proteins. Partial control of S. frugiperda in certain countries is due to its natural tolerance to the Bt bacterium. Of the 31 pests targeted and controlled by Bt crops in Latin America, only S. frugiperda has shown tolerance to certain Bt proteins in growers' fields, the most reliable indication of the status of Bt-susceptibility in most of the American continent.


Assuntos
Insetos/fisiologia , Controle Biológico de Vetores/estatística & dados numéricos , Animais , Bacillus thuringiensis/química , Resistência a Inseticidas/genética , América Latina , Plantas Geneticamente Modificadas
18.
Genome Announc ; 4(3)2016 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-27340058

RESUMO

Here, we report the genome sequence of Proteus mirabilis Pr2921, a uropathogenic bacterium that can cause severe complicated urinary tract infections. After gene annotation, we identified two additional copies of ucaA, one of the most studied fimbrial protein genes, and other fimbriae related-proteins that are not present in P. mirabilis HI4320.

19.
Rev. andal. med. deporte ; 9(1): 17-22, mar. 2016. tab
Artigo em Inglês | IBECS | ID: ibc-149385

RESUMO

Objective. The purpose was to investigate the factors related to aerobic fitness levels of adolescents in southern Brazil. Methods. The sample consisted of 1081 students (545 males and 536 females), with mean age of 12.8 (± 1.2) years. This is a cross-sectional epidemiological study. Aerobic fitness was assessed by the 9-min running test. The independent variables analyzed were: age, socioeconomic status, school system (public/private), sedentary behavior, physical activity level, nutritional status and body adiposity. Analyses were stratified by sex. We used the Student 't' test for independent samples and logistic regression to estimate odds ratio (OR) and confidence intervals of 95% (95% CI). Results. The prevalence of low aerobic fitness levels was 92.5%. Boys aged 13–14 years were 2.44 times more likely to have low aerobic fitness levels; poorer boys were 4.31 times more likely to have low aerobic fitness levels. Girls aged 13–14 years were 5.35 times more likely to have low aerobic fitness levels; poorer girls were 10.70 times more likely to have low aerobic fitness levels; girls with sedentary behavior were approximately 4 times more likely to have low aerobic fitness levels. Conclusion. Thus, it was concluded that almost all adolescents show low aerobic fitness levels, which makes necessary effective interventions in the school environment with the promotion of sports and regular practice of physical activity (AU)


Objetivo. El presente estudio tuvo como objetivo investigar los factores relacionados con los niveles de aptitud aeróbica de los adolescentes en el sur de Brasil. Métodos. La muestra estuvo conformada por 1081 alumnos (545 varones y 536 mujeres), con edad media de 12.8 (± 1.2) años. Se trata de un estudio epidemiológico transversal. La condición física aeróbica se evaluó mediante Test de Caminata de 9 minutos. Las variables independientes analizadas fueron: edad, nivel socioeconómico, el sistema escolar (público/privado), el sedentarismo, el nivel de actividad física, el estado nutricional y la adiposidad corporal. Los análisis fueron estratificados por sexo. Se aplicó la prueba de 't' Student para muestras independientes y regresión logística para estimar la odds ratio (OR) y los intervalos de confianza de 95% (IC95%). Resultados. La prevalencia de bajos niveles de aptitud aeróbica fue de 92.5%. Los niños de edades de 13 a 14 años fueron 2.44 veces más propensos a tener bajos niveles de condición física aeróbica; los niños más pobres fueron 4.31 veces más propensos a tener bajos niveles de condición física aeróbica. Las niñas de 13 a 14 años fueron 5.35 veces más propensas, a tener bajos niveles de condición física aeróbica; las niñas más pobres eran 10.70 veces más propensas a tener bajos niveles de condición física aeróbica; las niñas con el comportamiento sedentario, fueron aproximadamente 4 veces más propensas a tener bajos niveles de condición física. Conclusión. Se concluyó que casi todos los adolescentes muestran bajos niveles de condición física aeróbica, lo que hace necesarias intervenciones eficaces en el ámbito escolar para la promoción del deporte y la práctica regular de actividad física (AU)


Objetivo. O objetivo foi investigar os fatores relacionados aos níveis de aptidão aeróbia dos adolescentes no Sul do Brasil. Métodos. A amostra foi constituída por 1081 estudantes (545 do sexo masculino e 536 do sexo feminino), com idade média de 12.8 (± 1.2) anos. Este é um estudo epidemiológico transversal. Aptidão aeróbica foi avaliada pelo teste de corrida de 9 minutos. As variáveis independentes analisadas foram: idade, nível socioeconômico, sistema de escola (pública/privada), comportamento sedentário, nível de atividade física, estado nutricional e adiposidade corporal. As análises foram estratificadas por sexo. Foi utilizado o teste de Student 't' para amostras independentes e regressão logística para estimar odds ratio (OR) e intervalos de confiança de 95% (CI95%). Resultados. A prevalência de baixos níveis de aptidão aeróbia foi de 92.5%. Meninos com idades entre 13-14 anos foram 2.44 vezes mais propensos a ter baixos níveis de aptidão aeróbia; meninos mais pobres eram 4.31 vezes mais propensos a ter baixos níveis de aptidão aeróbia. Meninas com idades entre 13-14 anos foram 5.35 vezes mais propensos a ter baixos níveis de aptidão aeróbia; as moças pobres foram 10.70 vezes mais propensas a ter baixos níveis de aptidão aeróbia; Meninas com comportamento sedentário foram aproximadamente 4 vezes mais propensas a ter baixos níveis de aptidão aeróbia. Conclusão. Assim, concluiu-se que quase todos os adolescentes apresentam baixos níveis de aptidão aeróbica, o que faz intervenções eficazes no ambiente escolar com a promoção do desporto e da prática regular de atividade física necessária (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Exercício Físico/psicologia , Técnicas de Exercício e de Movimento/educação , Técnicas de Exercício e de Movimento/psicologia , Brasil , Estilo de Vida , Estado Nutricional/genética , Atividade Motora/genética , Comportamento Sedentário , Desempenho Atlético/educação , Exercício Físico/fisiologia , Técnicas de Exercício e de Movimento/métodos , Técnicas de Exercício e de Movimento/normas , Brasil/etnologia , Estado Nutricional/fisiologia , Atividade Motora/fisiologia , Comportamento Sedentário/etnologia , Desempenho Atlético/psicologia
20.
Rev. chil. obstet. ginecol ; 81(1): 63-85, feb. 2016. ilus, tab
Artigo em Espanhol | LILACS-Express | LILACS | ID: lil-775525

RESUMO

En la actualidad, la selección y uso correcto de los diferentes métodos de imágenes disponibles (ecografía, tomografía axial computada y resonancia magnética) constituye uno de los pilares fundamentales para el adecuado proceso diagnóstico en ginecología. Para la mayoría de las patologías benignas que comprometen al aparato genital femenino, la ecografía transvaginal es una herramienta sensible, de fácil acceso, costo-efectiva y por tanto debiese constituir el método inicial de elección. Sin embargo, su eficacia depende de que el operador sea entrenado y experimentado en su interpretación. El uso de la tomografía computada (TC) y la resonancia magnética (RM) debiese restringirse a condiciones específicas, en especial para caracterizar lesiones que no son completamente evaluables por ultrasonido. La mayor utilidad de la TC está en el diagnóstico diferencial de las urgencias ginecológicas, en evaluar el compromiso extra pelviano de neoplasias anexiales y en el estudio preoperatorio de anomalías de la vía urinaria. Respecto de la RM, su mayor utilidad está en el estudio de lesiones anexiales complejas a la ultrasonografía, endometriosis pélvica profunda y extra pelviana, adenomiosis, malformaciones uterinas, etapificación local de neoplasias endometriales, cervicales y vaginales, y en la evaluación de miomas múltiples o extra pelvianos.


In gynecology, the selection and proper use of the different available imaging methods (ultrasound, computed tomography and magnetic resonance) is paramount for a proper diagnostic process. For most benign conditions affecting the female genital tract, transvaginal ultrasound constitutes an exam with has excellent sensitive, is readily accessible and cost-effective, therefore being the initial method of choice. However, its effectiveness depends on the operator training and expertise in interpretation. The use of computed tomography (CT) and magnetic resonance imaging (MRI) ought to be restricted for specific conditions, especially to characterize lesions not completely evaluated by ultrasound. The main benefit of CT is in the differential diagnosis of gynecological emergencies, in assessing extra pelvic extension of adnexal masses and in the preoperative assessment of urinary tract abnormalities. Regarding MRI it is most useful in the study of complex adnexal lesions, extra pelvic and deep pelvic endometriosis, adenomyosis, uterine malformations, local staging of endometrial, cervical and vaginal neoplasms, and multiple or extra pelvic leiomyomas.

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