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1.
Fish Shellfish Immunol ; 96: 152-160, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31794843

RESUMO

C-type lectins are Ca2+-dependent carbohydrate-binding proteins containing one or more carbohydrate-recognition domains (CRDs). C-type lectins play crucial roles in innate immunity, including nonself-recognition and pathogen elimination. In the present study, two C-type lectins (designated ReCTL-1 and ReCTL-2) were identified from the shrimp Rimicaris exoculata which dwells in deep-sea hydrothermal vents. The open reading frames of ReCTL-1 and ReCTL-2 encoded polypeptides of 171 and 166 amino acids respectively, which were both composed of a signal peptide and a single CRD. The key motifs determining the carbohydrate binding specificity of ReCTL-1 and ReCTL-2 were respectively Glu-Pro-Ala (EPA) and Gln-Pro-Asn (QPN), which were firstly discovered in R. exoculata. ReCTL-1 and ReCTL-2 displayed similar pathogen-associated molecular pattern (PAMP) binding features and they bound three PAMPs-ß-glucan, lipopolysaccharide and peptidoglycan-with relatively high affinity. In addition, both could efficiently recognize and bind Gram-positive bacteria, Gram-negative bacteria and fungi. However, ReCTL-1 and ReCTL-2 exhibited different microbial agglutination activities: ReCTL-1 agglutinated Staphylococcus aureus and Saccharomyces cerevisiae, while ReCTL-2 agglutinated Micrococcus luteus, Vibrio parahaemolyticus and V. fluvialis. Both ReCTL-1 and ReCTL-2 inhibited the growth of V. fluvialis. All these results illustrated that ReCTL-1 and ReCTL-2 could function as important pattern-recognition receptors with broad nonself-recognition spectra and be involved in immune defense against invaders, but their specificities are not the same. In addition, the two ReCTLs possessed different carbohydrate binding specificities from each other and from the classical pattern: ReCTL-1 with an EPA motif bound d-galactose and l-mannose, while ReCTL-2 with a QPN motif bound d-fucose and N-acetylglucosamine.

2.
Biosci Rep ; 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31825467

RESUMO

Changes in dietary vitamin C intake have been related to the risks of various cancers. However, the association between dietary vitamin C intake and the risk of ovarian cancer has not been fully determined. A meta-analysis was performed to evaluate the relationship between vitamin C intake and ovarian cancer risk. Observational studies that evaluated the association between vitamin C intake and ovarian cancer risk were identified via systematic search of PubMed and Embase databases. A random effect model was used to combine relative risk (RR) with corresponding 95% confidence intervals (CI). As a result, 16 studies (5 cohort studies and 11 case-control studies) with 4,553 cases and 439,741 participants were included. Pooled results showed that dietary vitamin C intake had non-significant association on the risk of ovarian cancer (RR=0.95, 95%CI= 0.81-1.11, I2= 52.1%, Pfor heterogeneity= 0.008). Subgroup analyses according to characteristics including geographic location and study design showed consistent results with the overall result. In summary, findings from this study indicated that dietary vitamin C intake is not associated with the risk of ovarian cancer.

3.
Ther Clin Risk Manag ; 15: 1377-1381, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31819464

RESUMO

Background/Aim: Cesarean scar pregnancy is a long term complication of cesarean section. There is a lot of controversy about the best treatment methods. We retrospectively summarized the clinical characteristics of patients with cesarean scar pregnancy and explored the advantages and disadvantages of fertility-preservation treatment method. Methods: From January 2008 to September 2017, a total of 204 cases of cesarean scar pregnancy were retrospectively reviewed. 145 patients underwent transvaginal clearance, 33 patients underwent endoscopic surgery, and 26 patients underwent uterine artery embolism. The clinical characteristics, diagnosis, various treatment methods, and clinical outcomes were analyzed. Results: There were no significant differences among the three groups in terms of patient age, number of previous cesarean sections, serum human chorionic gonadotropin, and clinical symptoms. The difference in mean gestational sac diameter (23.5±2.1mm vs 31.3±2.4mm vs 30.8± 1.9mm), surgical time (31.4±2.5min vs. 45.8±2.2min vs. 51.4±1.9min), blood loss (53.3± 5.5mL vs. 105.2±3.2mL vs. 75.6 ±3.5mL), blood transfusion (1/145 case vs.3/33 case vs. 0/26 case), discomfort (1/145 case vs.9/33 case vs. 16/26 case), hospital stay (6.1±1.1 day vs. 7.4±0.9 day vs.18.6±1.5 day), fever duration (1.0±0.5 day vs. 2.1±2.8 day vs. 5.7±3.5 day), and hospital expense (¥ 7825.9±234.9 vs. ¥ 10248.3± 312.9 vs. ¥ 18774.9±243.6) in transvaginal pregnancy tissue clearance, endoscopic surgery, and uterine artery embolism groups were significantly different. Conclusion: Transvaginal clearance is an effective and relatively safe treatment option for patients with cesarean scar pregnancy.

4.
J Clin Lab Anal ; 33(8): e22959, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31241209

RESUMO

BACKGROUND: Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP) has shown to be overexpressed in several human cancers. The purpose of this study was to explore the expression of HPIP in endometrial cancer (EC) and its associated effects on disease. METHODS: A total of 113 EC patients at the Harbin Medical University Cancer Hospital between August 2011 and September 2012 were studied for immunohistochemistry analysis. HPIP expression was detected using real-time reverse transcription PCR, Western blotting, and immunohistochemistry. Prognostic value of HPIP expression was examined using multivariate Cox regression analysis and Kaplan-Meier method. RESULTS: The result of Western blotting indicated that HPIP protein expression is significantly high in normal tissues compared to EC tissues (P < 0.001). The expression of HPIP was significantly associated with FIGO stage (P < 0.001), histological grade (P < 0.001), depth of myometrial invasion (P < 0.001), and lymph node metastasis (P = 0.033). Kaplan-Meier analysis demonstrated that there was a significant difference in overall survival and disease-free survival between the two groups of patients stratified by HPIP expression level (log-rank, both P = 0.002). Patients with HPIP high expression had significantly shorter median survival time than those with HPIP low expression. Moreover, results of the multivariate analysis revealed that HPIP expression was an independent prognostic factor for predicting overall survival (P = 0.015) and disease-free survival (P = 0.017) in patients with EC. CONCLUSION: The present study provides evidence that HPIP predicts EC progression and poor survival, highlighting its potential as a therapeutic target for EC.

5.
BMC Mol Biol ; 20(1): 5, 2019 02 12.
Artigo em Inglês | MEDLINE | ID: mdl-30755162

RESUMO

BACKGROUND: GTPase-activating proteins (GAPs) with a TBC (Tre-2/Bub2/Cdc16) domain architecture serve as negative regulators of Rab GTPases. The related crystal structure has been studied and reported by other members of our research group in 2017 (Chen et al. in Protein Sci 26(4):834-846, 2017). The protein crystal structure and sequencing data accession numbers in Protein structure database (PDB) are 5TUB (Shark TBC1D15 GAP) and 5TUC (Sus TBC1D15 GAP), respectively. In this paper, we analyzed the Rab-GAP specificity of TBC1D15 in the evolution and influence of key amino acid residue mutations on Rab-GAP activity. RESULTS: Sequence alignment showed that five arginine residues of the TBC1D15-GAP domain are conserved among the species Sus/Mus/Homo but have been replaced by glycine or lysine in Shark. A fragment activity assay was conducted by altering the five residues of Shark TBC1D15-GAP to arginine, and the corresponding arginine in TBC1D15 GAP domains from Sus and Homo species were mutated to resemble Shark TBC1D15-GAP. Our data revealed that the residues of G28, K45, K119, K122 and K221 in the Shark TBC1D15-GAP domain had a key role in determining the specificity for Rab7 and Rab11. Mutation of the five residues significantly altered the Shark TBC1D15-GAP activity. CONCLUSIONS: These results revealed that the substrate specificity of TBC1D15 has had different mechanisms across the evolution of species from lower-cartilaginous fish to higher mammals. Collectively, the data support a different mechanism of Shark TBC1D15-GAP in substrate selection, which provides a new idea for the development of Marine drugs.


Assuntos
Sequência Conservada , Evolução Molecular , Proteínas Ativadoras de GTPase/química , Tubarões/metabolismo , Proteínas rab de Ligação ao GTP/química , Sequência de Aminoácidos , Animais , Arginina/química , Arginina/genética , Cristalografia por Raios X , Glicina/química , Glicina/genética , Humanos , Lisina/química , Lisina/genética , Camundongos , Mutação , Domínios Proteicos , Alinhamento de Sequência , Tubarões/genética , Especificidade por Substrato , Suínos , Proteínas rab de Ligação ao GTP/genética
6.
Fish Shellfish Immunol ; 84: 704-710, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30359751

RESUMO

Japanese eel (Anguilla japonica) has become a commercially important fish species all over the world. High-density aquaculture has led to congestion and contributed to bacterial infection outbreaks that have caused high mortality. Therefore a 56-days feeding trial was conducted to determine the effects of dietary Bacillus amyloliquefaciens (GB-9) and Yarrowia lipolytica lipase2 (YLL2) on growth performance, digestive enzymes activity, innate immunity and resistance to pathogens of A. japonica. Fish growth performance was significantly affected by dietary YLL2 supplementation but not by GB-9. Fish fed diets with YLL2 at 2.0 g/kg diet in combination of high and low levels of GB-9 (5.0 g/kg and 2.0 g/kg) produced the highest growth. For digestive enzyme, lipase and trypsin activities was promoted by dietary containing YLL2, while amylase activities was increased by dietary containing YLL2, GB-9 single or combination. For innate immunity, the mucus lysozyme activity, leukocytes phagocytosis activity and reactive oxygen species level of skin, peroxidase and lysozyme activity of serum were enhanced in fish fed with GB-9 compared to those in control group (p < 0.05). The highest resistance to Vibrio anguillarum and Aeromonas hydrophila was determined in fish fed with 5.0 g kg-1 GB-9 + 2.0 g/kg YLL2. This study demonstrated that GB-9 and YLL2 enhanced non-specific immune defense system of A. japonica, providing them with higher resistance to pathogens. The present results suggested that the combination of these supplements could be considered as potential biological additives for aquaculture farmed fish.


Assuntos
Anguilla/imunologia , Bacillus amyloliquefaciens/química , Hidrolases de Éster Carboxílico/administração & dosagem , Doenças dos Peixes/imunologia , Proteínas Fúngicas/administração & dosagem , Imunidade Inata/efeitos dos fármacos , Imunidade nas Mucosas/efeitos dos fármacos , Probióticos/farmacologia , Aeromonas hydrophila/fisiologia , Anguilla/crescimento & desenvolvimento , Anguilla/metabolismo , Animais , Dieta/veterinária , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Trato Gastrointestinal/enzimologia , Infecções por Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/veterinária , Distribuição Aleatória , Vibrio/fisiologia , Vibrioses/imunologia , Vibrioses/veterinária
7.
Fish Shellfish Immunol ; 82: 250-257, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30125703

RESUMO

A 12-weeks feeding trial was performed to investigate the possible effects of supplementation of Hybrid sturgeon diet with Bacillus amyloliquefaciens (GB-9) and Yarrowia lipolytica lipase2 (YLL2) single or combined on immune response and growth performance of Hybrid sturgeon (Acipenser schrenkii ♂and Acipenser baeri ♀). For this aim, Hybrid sturgeons were fed with four experimental diets namely: Diet 1 (0-control), Diet 2 (5.0 g/kg GB-9), Diet 3 (4.0 g/kg YLL2), and Diet 4 (5.0 g/kg GB-9 + 4.0 g/kg YLL2), respectively. After fed with varied diets, growth performance, mucosal immune response, leukocytes immune response and serum immunological response were measured. The results indicated that supplementations of GB-9 + YLL2 resulted in a significant increase in final weight, Docosahexaenoic acid (DHA) and Eicosapentenoic acid (EPA) concentration, compared with that of control (p < 0.05). For innate immunity, the results showed that skin mucus lysozyme activity, leukocytes phagocytosis activity and reactive oxygen species level, and serum alternative complement pathway activity, peroxidase and lysozyme activity were significantly higher in supplemented groups compared to the control (p < 0.05). The highest values were recorded in fish fed both YLL2 and GB-9 with respect to the individual application. The present results suggested that the combination of these supplementation could be considered as potential feed-additives for aquaculture farmed fish.


Assuntos
Bacillus amyloliquefaciens/química , Hidrolases de Éster Carboxílico/administração & dosagem , Peixes/crescimento & desenvolvimento , Peixes/imunologia , Proteínas Fúngicas/administração & dosagem , Probióticos/farmacologia , Ração Animal/análise , Animais , Cruzamento , Dieta/veterinária , Suplementos Nutricionais/análise , Imunidade Inata/efeitos dos fármacos , Imunidade nas Mucosas/efeitos dos fármacos , Distribuição Aleatória
8.
AMB Express ; 8(1): 84, 2018 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-29785529

RESUMO

The abuse of antibiotics and following rapidly increasing of antibiotic-resistant pathogens is the serious threat to our society. Natural products from microorganism are regarded as the important substitution antimicrobial agents of antibiotics. We isolated a new strain, Bacillus sp. GFP-2, from the Chiloscyllium plagiosum (Whitespotted bamboo shark) intestine, which showed great inhibitory effects on the growth of both Gram-positive and Gram-negative bacteria. Additionally, the growth of salmon was effectively promoted when fed with inactivated strain GFP-2 as the inhibition agent of pathogenic bacteria. The genes encoding antimicrobial peptides like LCI, YFGAP and hGAPDH and gene clusters for secondary metabolites and bacteriocins, such as difficidin, bacillibactin, bacilysin, surfactin, butirosin, macrolactin, bacillaene, fengycin, lanthipeptides and LCI, were predicted in the genome of Bacillus sp. GFP-2, which might be expressed and contribute to the antimicrobial activities of this strain. The gene encoding ß-1,3-1,4-glucanase was successfully cloned from the genome and this protein was detected in the culture supernatant of Bacillus sp. GFP-2 by the antibody produced in rabbit immunized with the recombinant ß-1,3-1,4-glucanase, indicating that this strain could express ß-1,3-1,4-glucanase, which might partially contribute to its antimicrobial activities. This study can enhance a better understanding of the mechanism of antimicrobial activities in genus Bacillus and provide a useful material for the biotechnology study in antimicrobial agent development.

9.
Fish Shellfish Immunol ; 78: 238-247, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29678793

RESUMO

Galectins are ß-galactoside binding lectins that play crucial roles in innate immunity in vertebrates and invertebrates through their conserved carbohydrate-recognition domains (CRDs). In the present study, single- and four-CRD-containing galectins were identified in oyster Crassostrea gigas (designated CgGal-2 and CgGal-3). The open reading frames (ORFs) of CgGal-2 and CgGal-3 encode polypeptides of 200 and 555 amino acids, respectively. All CRDs of CgGal-3 include two consensus motifs essential for ligand-binding, and a novel motif is present in CgGal-2. Pathogen-associated molecular pattern (PAMP) profiles were determined for recombinant rCgGal-2 and rCgGal-3, and rCgGal-2 displayed low binding affinity for PAMPs, while rCgGal-3 bound various PAMPs including glucan, lipopolysaccharide (LPS), and peptidoglycan (PGN) with relatively high affinity. Furthermore, rCgGal-2 and rCgGal-3 exhibited different microbe binding profiles; rCgGal-2 bound to Gram-negative bacteria (Escherichia coli and Vibrio vulnificus) and fungi (Saccharomyces cerevisiae and Pichia pastoris), while rCgGal-3 bound to these microbes but also to Gram-positive bacteria (Micrococcus luteus). In addition, rCgGal-3 possessed microbial agglutinating activity and coagulation activity against fungi and erythrocytes, respectively, but rCgGal-2 lacked any agglutinating activity. Carbohydrate binding specificity analysis showed that rCgGal-3 specifically bound D-galactose. Furthermore, rCgGal-2 and rCgGal-3 functioned as opsonin participating in the clearance against invaders in C. gigas. Thus, CgGal-2 with one CRD and CgGal-3 with four CRDs are new members of the galectin family involved in immune responses against bacterial infection. Differences in the organisation and amino acid sequences of CRDs may affect their specificity and affinity for nonself substances.


Assuntos
Crassostrea/genética , Galectina 2/genética , Galectina 3/genética , Regulação da Expressão Gênica/imunologia , Imunidade Inata/genética , Sequência de Aminoácidos , Animais , Crassostrea/imunologia , Fungos/fisiologia , Galectina 2/química , Galectina 2/imunologia , Galectina 3/química , Galectina 3/imunologia , Perfilação da Expressão Gênica , Bactérias Gram-Negativas/fisiologia , Bactérias Gram-Positivas/fisiologia , Filogenia , Alinhamento de Sequência
10.
Oncol Lett ; 15(3): 2801-2806, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29435007

RESUMO

Lung cancers are the most commonly diagnosed malignant tumors, and are one of the leading causes of morbidity and mortality worldwide. Dexamethasone (DEX) serves an important function in the regulation of lung cancer cell proliferation; however, the mechanisms involved still remain unknown. In the present study, the effects of DEX on A549 cell proliferation and apoptosis were examined, in addition to the potential downstream regulatory mechanisms underlying these effects. A549 cells were treated with different concentrations of DEX at 12, 24 and 48 h time points, followed by the addition of SB431542, an inhibitor of the TGF-ß1 receptor, to block the TGF-ß1 signaling pathway. Cell proliferation was analyzed using a 3-(4,5-diethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt. The apoptosis rate was measured by Hoechst 33342 and Annexin V/propidium iodide staining and the expression of transforming growth factor (TGF)-ß1, Smad family member 2 (Smad2) and caspase-3 were assessed by western blot. The results from the present study demonstrated that the proliferation of A549 cells decreased and the apoptosis rate significantly increased following DEX treatment (P<0.05). Furthermore, the expression of TGF-ß1, Smad2 and caspase-3 were significantly increased following DEX stimulation (P<0.05), the effects of which were abrogated by the addition of the TGF-ß1 receptor inhibitor, SB431542 (P<0.05). DEX-induced apoptosis in A549 cells, and this effect was abrogated by SB431542, an inhibitor of TGF-ß1 receptor signaling, which indicated that the TGF-ß1/Smad2 pathway may be associated with this process and SB431542 may function as an antitumor drug in the future.

11.
Biochem Biophys Res Commun ; 460(3): 826-30, 2015 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-25824041

RESUMO

2-Deoxyribose aldolase-catalyzed C-C bond-forming reactions have become 1 more and more important in synthesis of statins and other drug intermediates. Many methods have focused on improving the aldolase properties and harvesting new aldolases, but a good outcome depends on the efficiency of the high-throughput screening system. We have developed a visible green fluorescence probe based on a coumarin derivative, which can be reversibly modulated by a retro-aldol reaction catalyzed by 2-deoxyribose aldolase for selecting aldolase mutants with high activity. This assay system provides a convenient and effective way for high-throughput screening aldolases as the green fluorescence is sensitively detected and daylight-viewable without the need for specialist equipment. We used our probe to successfully harvest aldolase mutants with higher activities than the parent from a random mutagenesis library.


Assuntos
Aldeído Liases/química , Desoxirribose/química , Corantes Fluorescentes/química , Ensaios de Triagem em Larga Escala , Primers do DNA , Ressonância Magnética Nuclear Biomolecular , Reação em Cadeia da Polimerase , Espectrometria de Fluorescência
12.
Neuroreport ; 25(17): 1338-43, 2014 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-25304496

RESUMO

Sodium valproate (VPA) is widely used as an antiepileptic agent and mood stabilizer. In recent years, VPA has been increasingly used as a psychotherapeutic drug to treat depression. In this article, a possible antidepressant mechanism of VPA was investigated by studying the expression and therefore the involvement of tryptophan hydroxylase, serotonin transporter (5-HTT), monoamine oxidase-A (MAO-A), and indoleamine 2, 3-dioxygenase (IDO) in rats exposed to chronic unpredicted stress. Male Sprague-Dawley rats were divided into four groups: the vehicle-treated control group (CG), the VPA-treated control group (VPAC), the vehicle-treated model group (MG), and the VPA-treated model group (VPAM). VPA (300 mg/kg once daily) was administered to VPAC and VPAM rats by means of intragastric gavage while an equivalent volume of vehicle was given to vehicle-treated CG and MG rats. Rat behavior and expression of tryptophan hydroxylase, 5-HTT, MAO-A, and IDO in the hippocampus were determined. A significant reduction in depression-like behaviors was observed with an upregulation of 5-HTT expression and a downregulation of MAO-A and IDO expression in VPAM rats, compared with MG rats. The results may suggest that the antidepressant mechanism of VPA is partly related to elevated serotonin level and its reuse in the vesicles of presynaptic nerve endings.


Assuntos
Antidepressivos/farmacologia , Transtorno Depressivo/tratamento farmacológico , Transtorno Depressivo/fisiopatologia , Animais , Doença Crônica , Modelos Animais de Doenças , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Indolamina-Pirrol 2,3,-Dioxigenase/metabolismo , Masculino , Monoaminoxidase , RNA Mensageiro/metabolismo , Distribuição Aleatória , Ratos Sprague-Dawley , Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo , Estresse Psicológico , Triptofano Hidroxilase/metabolismo , Ácido Valproico/farmacologia
13.
Eur J Obstet Gynecol Reprod Biol ; 176: 25-30, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24630298

RESUMO

OBJECTIVES: To establish red fluorescent human endometriosis lesions in a nude mouse model and dynamically and non-invasively to compare intraperitoneal and subcutaneous injection models. STUDY DESIGN: Primary cultures of endometrial stromal cells (ESCs) and epithelial cells (EECs) isolated from 24 patients with a normal uterine cavity were transfected with 2.5×10(8) (Group 1) and 1.25×10(8) (Group 2) plaque-forming units (PFU) of adenovirus encoding red fluorescent protein (Ad-RFP). Transfection efficiencies, fluorescence intensity and apoptosis rate of the two types of cells were compared in vitro. A mixture of 2.5×10(8) PFU Ad-RFP-infected approximately 400 EECs cell mass and 2×10(6) ESCs for 36h was injected individually into 24 female nude mice subcutaneously (Group A) or intraperitoneally (Group B). From Day 5 after injection, an in vivo imaging system (IVIS) was used to non-invasively observe and compare the lesions of the two groups every week until Day 33. Specifically, the fluorescent intensity, positive rates, persistence time and lesion weight in the implanted human endometriosis lesions were compared. A parametric Student's t-test and two-way analysis of variance were used for statistical analysis. RESULTS: Compared with 1.25×10(8) PFU RFP, a titre of 2.5×10(8) PFU RFP ESCs and EECs incubated for 36h exhibited higher transfection efficiencies and higher fluorescence intensities in vitro. In vivo imaging of the fluorescent human endometriosis lesions originating from an RFP titre of 2.5×10(8) PFU showed that the intensity and lesion weight in Group A were significantly higher than in Group B. However, the two groups had the same RFP-positive rates and fluorescence persistence. The structure of each lesion was evaluated by immunohistochemistry to confirm its human endometrial origin. CONCLUSIONS: The red fluorescent human endometriosis model established by subcutaneously injecting 2.5×10(8) PFU RFP-transfected stromal cells and epithelial cells into nude mice had a higher fluorescent positive rate from Day 5, higher intensity and weight but the same persistence as the intraperitoneal injection model.


Assuntos
Endometriose/patologia , Proteínas Luminescentes/genética , Adulto , Animais , Células Epiteliais/metabolismo , Feminino , Humanos , Injeções Subcutâneas , Camundongos Nus , Pessoa de Meia-Idade , Células Estromais/metabolismo , Transfecção
14.
Exp Biol Med (Maywood) ; 239(3): 330-6, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24495952

RESUMO

Histone acetylation has been linked to depression, the etiology of which involves many factors such as genetics, environments, and epigenetics. The aim of the present study was to investigate whether it was associated with epigenetic histone modification and gene expression of enzymes responsible for the biosynthesis of norepinephrine and serotonin in rat depression model induced by chronic unpredictable stress (CUS). Eight-week-old male Sprague-Dawley rats were exposed to CUS over 28 days. It was shown that the CUS-induced rats displayed remarked anxiety- and depression-like behavior with weakened locomotor activity in open field test and prolonged immobility in forced swimming test. Western blot revealed that CUS led to significant decrease in acetylation of H3 at Lysine 9 (K9) and H4 at Lysine 12 (K12) with obviously increasing histone deacetylases 5 (HDAC5) expression in hippocampus of CUS-induced rats. Meanwhile, there was an obviously decreased expression of tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH) both at protein and mRNA levels. Administration of sodium valproate (VPA), a histone deacetylase 5 (HDAC5) inhibitor, not only significantly relieved the anxiety- and depression-like behaviors of CUS-induced rats but also clearly blunted decrease of H3(K9) and H4(K12) acetylation and expression of TH and TPH, and prevented increase of HDAC5 expression. The results indicate that there exists possible interrelation between TH and TPH gene expression and epigenetic histone acetylation in CUS-induced depressive rats, which at least partly contributes to the etiology of depression.


Assuntos
Depressão/enzimologia , Epigênese Genética , Estresse Psicológico/complicações , Triptofano Hidroxilase/metabolismo , Tirosina 3-Mono-Oxigenase/metabolismo , Acetilação/efeitos dos fármacos , Animais , Ansiedade/enzimologia , Ansiedade/etiologia , Ansiedade/genética , Depressão/etiologia , Depressão/genética , Modelos Animais de Doenças , Expressão Gênica , Hipocampo/metabolismo , Inibidores de Histona Desacetilases/farmacologia , Histona Desacetilases/biossíntese , Histona Desacetilases/metabolismo , Histonas/metabolismo , Masculino , Norepinefrina/biossíntese , Norepinefrina/genética , RNA Mensageiro/biossíntese , Ratos , Ratos Sprague-Dawley , Serotonina/genética , Triptofano Hidroxilase/biossíntese , Triptofano Hidroxilase/genética , Tirosina 3-Mono-Oxigenase/biossíntese , Tirosina 3-Mono-Oxigenase/genética , Ácido Valproico/farmacologia
15.
Neuroreport ; 25(4): 205-10, 2014 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-24128869

RESUMO

Sodium valproate (VPA) is an antiepileptic drug and mood stabilizer used to treat bipolar disorders. Recently, other psychiatric uses for VPA have been based on its antidepressive and neuroprotective effects. In the current work, the antidepressive mechanism of VPA was investigated by studying the expression of brain-derived neurotrophic factor (BDNF) and hypothalamic-pituitary-adrenal axis function in rats exposed to a protocol of chronic unpredicted stress (CUS). Male Sprague-Dawley rats were divided into a vehicle-treated control group (no CUS+vehicle), a VPA-treated control group (no CUS+VPA), a vehicle-treated model group (CUS+vehicle), and a VPA-treated model group (CUS+VPA). VPA (300 mg/kg once daily) was administered to rats (no CUS+VPA and CUS+VPA) by an intragastric gavage, whereas the same volume of vehicle was administered to rats in the no CUS+vehicle and CUS+vehicle groups. Rat behavior, serum corticosterone level, and expression of BDNF in the hippocampus and corticotrophin-releasing factor in the hypothalamus were determined. Compared with the CUS+vehicle rats, the CUS+VPA rats showed a significant relief in depression-like behaviors and a decrease in the corticosterone level and corticotropin-releasing factor expression with increasing expression of BDNF. The results suggest that the antidepressive effect of VPA is at least partly related to improving hypothalamic-pituitary-adrenal axis function and elevating the expression of BDNF.


Assuntos
Antidepressivos/farmacologia , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Hormônio Liberador da Corticotropina/metabolismo , Transtorno Depressivo/tratamento farmacológico , Transtorno Depressivo/metabolismo , Ácido Valproico/farmacologia , Animais , Corticosterona/sangue , Expressão Gênica/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Hipotálamo/efeitos dos fármacos , Hipotálamo/metabolismo , Masculino , Testes Neuropsicológicos , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Estresse Psicológico/tratamento farmacológico , Estresse Psicológico/metabolismo , Análise e Desempenho de Tarefas
16.
Environ Sci Technol ; 45(10): 4532-9, 2011 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-21500850

RESUMO

TiO(2) photocatalytic oxidation (PCO) of As(III) in the normal air-saturated aqueous solutions has been widely studied. Yet no consensus has been achieved on the mechanism whether superoxide is the main oxidant, although many approaches have been taken. (Photo)electrochemical method can minimize changes to TiO(2) surface and could therefore not alter the normal mechanism. In this Article, both this approach and As(III) oxidation kinetic measurements were performed to clarify the disputed mechanism. Under a sufficient cathodic bias potential, the dark oxidation of As(III) by superoxide could occur, but both the reaction rate and the columbic efficiency were rather low, suggesting that it is a weak oxidant. However, under UV light, both the reaction rate and the columbic efficiency were greatly enhanced even at potentials negative enough to eliminate photohole participation, indicating that more efficient oxidants than superoxide were produced. Under UV illumination and enough positive potential where superoxide was absent, the As(III) oxidation was the most highly efficient. The columbic efficiency of photoholes was much higher than that of superoxide. In the normal aerated aqueous solutions and at open circuit, although the total contribution of superoxide and its derivates to the PCO of As(III) was considerably high (up to 43%), it was not more than that of photohole (57%). In addition, the reported various approaches taken to elucidate the mechanism were discussed, and the resulting disputes can be clarified by these findings. It was demonstrated that (photo)electrochemical method could provide direct and undisputed evidence to reveal the truth mechanics issues.


Assuntos
Arsenitos/química , Superóxidos/química , Titânio/química , Poluentes Químicos da Água/química , Água Doce/química , Radical Hidroxila/química , Cinética , Oxirredução , Processos Fotoquímicos
17.
J Am Chem Soc ; 132(40): 14039-41, 2010 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-20849110

RESUMO

A global strategy to prepare a versatile and robust reactive platform for immobilizing molecules on carbon substrates with controlled morphology and high selectivity is presented. The procedure is based on the electroreduction of a selected triisopropylsilyl (TIPS)-protected ethynyl aryldiazonium salt. It avoids the formation of multilayers and efficiently protects the functional group during the electrografting step. After TIPS deprotection, a dense reactive ethynyl aryl monolayer is obtained which presents a very low barrier to charge transfer between molecules in solution and the surface. As a test functionalization, azidomethylferrocene was coupled by "click" chemistry with the modified surface. Analysis of the redox activity highlights a surface concentration close to the maximum possible attachment considering the steric hindrance of a ferrocenyl group.

18.
Huan Jing Ke Xue ; 29(7): 1937-41, 2008 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-18828380

RESUMO

Deactivation of Pt electrode in the process of electroxidation of p-chlorophenol (p-CP) was investigated using linear sweep voltammetry, LC/MS and spectrum analysis techniques. The experiment results indicated that Pt would lose its electro-catalytic activity soon because polymer formed at the electrode surface. The in-site IR spectra of Pt showed two weak absorption bands appeared at 1 200 and 1 800 cm(-1) during the oxidation of p-CP, which are characteristics of aromatic ether and carbonyl group, respectively. Increasing initial concentration of p-CP and pH value of solution would accelerate the deactivation speed of Pt. Acetonitrile lixivium for deactivated Pt was analyzed by LC/MS, and it was found that the polymers formed at the surface of Pt were some mixed compounds. The mechanism of polymerization includes the following ways: coupling reaction of organic radical each other; substituting reaction of organic radical with p-CP (or intermediates or small polymers).


Assuntos
Clorofenóis/química , Eletroquímica/métodos , Platina/química , Poluentes Químicos da Água/química , Eletroquímica/instrumentação , Eletrodos , Oxirredução
19.
Ergonomics ; 46(1-3): 271-84, 2003 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-12554411

RESUMO

A small-scale virtual system has been developed in this study to enhance operators' understanding and operating performance. For this, a computerized graphical interface based on Dynamic Work Causality Equation (DWCE) has been designed to transform the operating procedure into a flowchart. Furthermore, the Programmable Logic Controller (PLC) was installed to connect the signboard (proposed system) with the computerized graphical interface. An experiment was conducted to verify the effect of computerized graphic interface, indicating that the computerized system significantly decreases learning time and improves operational performance.


Assuntos
Sistemas de Computação/normas , Ergonomia , Centrais Elétricas/organização & administração , Gestão da Segurança , Interface Usuário-Computador , Gráficos por Computador , Simulação por Computador , Guias como Assunto , Humanos , Sistemas Homem-Máquina , Pesquisa Operacional , Linguagens de Programação , Desenho de Programas de Computador
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