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1.
Anal Bioanal Chem ; 2020 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-33164153

RESUMO

A UV-vis spectrometer, as a sort of important analytical instrument, has been widely used to analyze various substances. However, expensive equipment and skilled operators are required, which limits its broad applications for out-of-lab and daily measurements. In this work, a self-designed sensing device based on smart phone was developed as a sensitive, cost-effective, facile, and portable testing tool. The sensing device fabricated by 3D printing was used to lodge a sample solution and produce a light signal, and the optical sensor on a smart phone worked as a transducer. The light source in the device generated wide-wavelength radiation, which passed through an inner filter and only light of a designated wavelength reached the testing solution. The intensity of transmitted light was then measured by an optical sensor internally installed in most smart phones, where the signals were processed as well. The feasibility of our device was verified by detecting four kinds of common heavy metal ions in actual water samples, and the testing results showed good agreement with those obtained from the UV-vis spectrometer. This work is expected to shed some light on the construction of smart phone-based sensors, featuring decent portability, simple operation, low cost, high sensitivity, and good accuracy.

2.
Foods ; 9(11)2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33143312

RESUMO

Salmon is a highly perishable food due to temperature, pH, odor, and texture changes during cold storage. Intelligent monitoring and spoilage rapid detection are effective approaches to improve freshness. The aim of this work was an evaluation of IoT-enabled monitoring system (IoTMS) and electronic nose spoilage detection for quality parameters changes and freshness under cold storage conditions. The salmon samples were analyzed and divided into three groups in an incubator set at 0 °C, 4 °C, and 6 °C. The quality parameters, i.e., texture, color, sensory, and pH changes, were measured and evaluated at different temperatures after 0, 3, 6, 9, 12, and 14 days of cold storage. The principal component analysis (PCA) algorithm can be used to cluster electronic nose information. Furthermore, a Convolutional Neural Networks and Support Vector Machine (CNN-SVM) based algorithm is used to cluster the freshness level of salmon samples stored in a specific storage condition. In the tested samples, the results show that the training dataset of freshness is about 95.6%, and the accuracy rate of the test dataset is 93.8%. For the training dataset of corruption, the accuracy rate is about 91.4%, and the accuracy rate of the test dataset is 90.5%. The overall accuracy rate is more than 90%. This work could help to reduce quality loss during salmon cold storage.

3.
Ann Palliat Med ; 9(5): 3213-3221, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33065783

RESUMO

BACKGROUND: Gumi Bao decoction is effective for the treatment of osteoporosis, but the theoretical and scientific basis is unknown. This study aimed to observe the effect of Gumi Bao decoction on Dickkopf-related protein 1 (DKK1) mRNA, runt-related transcription factor 2 (Runx2) mRNA, and cathepsin K (CTSK) mRNA in glucocorticoid-induced osteoporosis (GIOP) in rats and to investigate the underlying mechanism. METHODS: Sixty Sprague-Dawley (SD) rats were weighed and randomly divided into six groups: the normal control group (NC group), the methylprednisolone group (Met group), the Fosamax group, the low-dose Gumi Bao Decoction group (GBDL group), the medium-dose Gumi Bao Decoction group (GBDM group), and the high-dose Gumi Bao Decoction group (GBDH group). The basic physiological conditions of the rats and the bone morphology of the fourth lumbar vertebra and the left femur of three rats in each group were observed, and the mRNA expressions of bone tissue-related genes were detected. RESULTS: After administration, DKK1 mRNA expression was significantly up-regulated in the Met group compared to the NC group (P<0.001). Meanwhile, DKK1 mRNA was significantly down-regulated in the Fosamax group compared with the Met group (P<0.001). There was a significant difference in the down-regulation of DKK1 mRNA between the GBDM and GBDH groups (P<0.001). Runx2 mRNA was considerably down-regulated in the Met group compared with the NC group (P<0.001). Runx2 mRNA was up-regulated in the GBDM group, and the GBDH group was significantly different compared to the Met group (P<0.001). CTSK mRNA was significantly up-regulated in the Met group compared to the NC group (P<0.001). Compared with the Met group, CTSK mRNA expression was significantly down-regulated in the Fosamax group (P<0.001), as well as in the GBDL, GBDM, and GBDH groups (P<0.001). At 200× and 400×, there were significantly fewer osteoblasts and osteoclasts in the Met group than in the Fosamax, GBDM, and GBDH groups. CONCLUSIONS: The imbalance of bone homeostasis of GIOP is caused by an increase in bone resorption and decreased osteogenesis. Gumi Bao could regulate bone metabolism through the action of DKK1 via the Wnt/ß-catenin signaling pathway, up-regulating Runx2 mRNA and down-regulating CTSK mRNA.

4.
Int Ophthalmol ; 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33078228

RESUMO

OBJECTIVE: The aim of this study is to clone the LpxA gene of Chlamydia trachomatis and analyze its biological characteristics. METHODS: Specific primers were designed according to the sequence of Ct LpxA gene. LpxA gene was amplified by PCR and connected to pMD18-T vectors. Positive clones were selected for PCR and DNA sequencing. Finally, bioinformatics software was used to analyze the biological properties of LpxA protein. RESULTS: The total length of LpxA gene was 840 bp, encoding 280 amino acids. LpxA protein has no signal peptide and was located in bacterial cytoplasm. The prediction of secondary structure showed that the α-helix, extended strand, ß-turn and random coil accounted for 19.6%, 32.8%, 11.4% and 36%, respectively. According to the prediction of tertiary structure, three identical LpxA molecules constituted homologous trimers. It was predicted that there were 11 B cell epitopes in LpxA. CONCLUSION: Ct Lpxa gene was cloned, and LpxA protein structure and function were predicted.

5.
Aging (Albany NY) ; 12(18): 18649-18659, 2020 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-32897242

RESUMO

Glioma is the most common malignant brain tumor. Because of its high degree of malignancy, the effect of surgical treatment, radiotherapy, chemotherapy, or immunotherapy is not ideal. TXNDC9 belongs to thioredoxin domain-containing proteins, which is involved in tumor progression. However, no research associated with TXNDC9 has been reported in glioma. In this study, we found that TXNDC9 was upregulated in glioma. Knockdown of TXNDC9 would prevent proliferation and metastasis, induce the apoptosis rate of glioma cells, and promote the expression Cleaved-caspase3, Cleaved-caspase8, Cleaved-caspase9. Meanwhile, knockdown of TXNDC9 induced autophagy by increasing the level of LC3 and Beclin-1. Cell morphology and expression analysis of GFAP, Vimentin, verified that TXNDC9 could regulate glioma cell differentiation. During this program, the expression of p53 changes dramatically. The apoptosis, autophagy, and cell differentiation program were blocked by p53 inhibitor treatment. In conclusion, the silencing of TXNDC9 induces apoptosis and autophagy in glioma and promotes cell differentiation by controlling p53 and may function as a new mechanism in glioma.

6.
Mikrochim Acta ; 187(9): 496, 2020 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-32803450

RESUMO

A novel electrochemical sensor based on dual-template molecularly imprinted polymer (MIP) with nanoporous gold leaf (NPGL) was established for the simultaneous determination of dopamine (DA) and uric acid (UA). NPGL acts as an enlarged loading platform to enhance sensing capacity, and the MIP layer was synthesized in situ in the presence of monomer and dual templates (DA and UA) to provide specific recognition. Under the optimal conditions, the sensor shows a good linear range of 2.0~180 µM for DA at a working potential of 0.15 V (vs. Ag/AgCl) and 5.0~160 µM for UA at 0.35 V (vs. Ag/AgCl), with the respective detection limit of 0.3 µM and 0.4 µM (S/N = 3). Good selectivity of the sensor to its dual templates was confirmed as the sensing signals are significantly different between templates and interfering species. The responses maintained higher than 96% of the initial values after 30-day storage, and the day-to-day relative standard deviation is less than 3.0%. Real sample simultaneous determination of DA and UA was conducted with bovine serum, and the results were in good agreement with those from high-performance liquid chromatography. It can be concluded that this work offers a reliable, facile, fast, and cost-effective method of simultaneous quantification of two or more chem-/bio-molecules. Graphical abstract.

7.
Mikrochim Acta ; 187(7): 380, 2020 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-32518983

RESUMO

The electrocatalytic activity of metal-organic framework (MOF)-derived NiO@ZnO hollow microspheres was studied for its application to an isoniazid sensor. The MOF-derived NiO@ZnO hollow spheres were synthesized by the coordination reaction of terephthalic acid with Zn2+ and Ni2+ and followed by calcination. Morphology characterization showed that the MOF-derived NiO@ZnO sphere has circular core-shell structure with pores on its surface. Further electrochemical characterization of the prepared sensor by cyclic voltammetry and differential pulse voltammetry proved that the material has good electrical conductivity and strong catalytic ability. Distinct oxidation peaks occur for INZ at potential of 0.22 V (vs. saturated calomel electrode). Under the optimal experimental conditions, the linear range of the sensor for isoniazid determination was 0.8 ~ 800 µM, and the detection limit was 0.25 µM (S/N = 3). In addition, the sensor displayed good stability, repeatability, and reproducibility. The established method was successfully applied for determination of isoniazid in tablets and mouse serum with admirable accuracy and reliability. Graphical abstract Schematic presentation of an electrochemical sensor based on MOF-derived NiO@ZnO hollow microspheres for isoniazid determination.

8.
Research (Wash D C) ; 2020: 7962024, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32566931

RESUMO

Surface-enhanced Raman spectroscopy (SERS) is a powerful sensing technique capable of capturing ultrasensitive fingerprint signal of analytes with extremely low concentration. However, conventional SERS probes are passive nanoparticles which are usually massively applied for biochemical sensing, lacking controllability and adaptability for precise and targeted sensing at a small scale. Herein, we report a "rod-like" magnetic nanomotor-based SERS probe (MNM-SP) that integrates a mobile and controllable platform of micro-/nanomotors with a SERS sensing technique. The "rod-like" structure is prepared by coating a thin layer of silica onto the self-assembled magnetic nanoparticles. Afterwards, SERS hotspots of silver nanoparticles (AgNPs) are decorated as detecting nanoprobes. The MNM-SPs can be navigated on-demand to avoid obstacles and target sensing sites by the guidance of an external gradient magnetic field. Through applying a rotating magnetic field, the MNM-SPs can actively rotate to efficiently stir and mix surrounding fluid and thus contact with analytes quickly for SERS sensing. Innovatively, we demonstrate the self-cleaning capability of the MNM-SPs which can be used to overcome the contamination problem of traditional single-use SERS probes. Furthermore, the MNM-SPs could precisely approach the targeted single cell and then enter into the cell by endocytosis. It is worth mentioning that by the effective mixing of intracellular biocomponents, much more informative Raman signals with improved signal-to-noise ratio can be captured after active rotation. Therefore, the demonstrated magnetically activated MNM-SPs that are endowed with SERS sensing capability pave way to the future development of smart sensing probes with maneuverability for biochemical analysis at the micro-/nanoscale.

9.
Z Naturforsch C J Biosci ; 75(9-10): 313-317, 2020 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-32374296

RESUMO

The purpose of this study is to purify the LpxA protein of Chlamydia trachomatis (Ct) and prepare the polyclonal antibody against LpxA protein, so as to lay a foundation for studying the function of LpxA protein. The LpxA gene was amplified by PCR. The expression plasmid pET28a-LpxA was constructed by using pET28a as the vector. The fusion protein containing 6 histidine tag was induced by IPTG and purified by Ni2+ chromatography gel. The purified His-LpxA protein was used as an immunogen to immunize New Zealand rabbits subcutaneously through the back to prepare polyclonal antibody. Immunoblotting was used to detect the reaction between the antibody and His-LpxA. The determination of polyclonal antibody titer was detected by ELISA. The relative molecular weight of His-LpxA was 32.8 kDa, and it could be expressed in Escherichia coli. The purity of the purified protein was about 95%. After immunizing New Zealand rabbits, the antiserum was able to recognize the recombinant His-LpxA protein with a titer greater than 1:10240. In this study, LpxA protein was successfully purified and antiserum was prepared, which provided an experimental basis for studying the function of LpxA protein.

10.
Int Ophthalmol ; 40(10): 2435-2440, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32399774

RESUMO

PURPOSE: To study the effect of curcumin on proliferation and invasion of the human retinoblastoma cells and its potential mechanism. METHODS: A cell line of retinoblastoma (WERI-Rb-1) was treated with various concentrations of curcumin (0-40 µM). Cell number was counted with CCK8 kit, and cell migration was assessed using the Transwell assay. Immunoblotting was performed to detect the proteins of metalloproteinase-2 (MMP-2), MMP-9 and vascular endothelial growth factor (VEGF) as well as nuclear translocation of nuclear factor-κB (NF-κB, p65). RESULTS: Proliferation and migration of WERI-Rb-1 cells were significantly inhibited by curcumin in a concentration-dependent manner (0-40 µM). Protein expressions of MMP-2, MMP-9 and VEGF in the WERI-Rb-1 cells were also significantly inhibited by curcumin in a concentration-dependent manner (0-40 µM). Furthermore, nuclear translocation of NF-κB (p65) was significantly inhibited by curcumin in time-dependent manner (6-24 h). CONCLUSION: Curcumin inhibited proliferation and migration of WERI-Rb-1 cells, a cell line of human retinoblastoma, which might be through modulating NF-κB and its downstream proteins including VEGF, MMP-2, and MMP-9.

11.
Nanotechnology ; 31(18): 185303, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31958779

RESUMO

Electromagnetic interference protection in optoelectronic devices is challenging because of the dual requirements of optical transmittance and high shielding effectiveness (SE). Herein, we propose a novel silver nanowire (AgNW)/polyethylene terephthalate (PET) multi-layer mesh pattern structure for transparent electromagnetic shielding obtained via laser marking and transfer printing. A three-layer composite shielding film with an optical transmittance of 67.8% exhibits a SE of 44 dB at 10 GHz, which is superior to most of the reported transparent shielding films composed of AgNWs to date. The newly designed multi-layer composite structure can enhance the transparent shielding properties of the shielding film via optimization of the AgNW distribution and the shielding film structure. It is expected that this multi-layer mesh composite structure will have splendid application prospects in electromagnetic shielding films, which require both light transmittance and high SE.

12.
Electrophoresis ; 41(16-17): 1418-1424, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31797398

RESUMO

Whispering gallery mode (WGM) resonators have received extensive attention because of their nonlinear optical application in lasers and sensors. Optical microcavities are excellent candidates for constructing powerful microlasers and label-free biosensors, owing to their low optical losses and small size. However, most of these microcavity syntheses rely on sophisticated fabrication methods and cannot be manipulated easily. To achieve facile and versatile microcavity fabrication, we present a robust microfluidics method for monodispersed self-coupling optical microcavity fabrication with a fine tunability. The microcavity polydispersity was less than 3%. The optical microcavity size could be varied from 10 to 30 µm with a steady quality factor (Q) of approximately 1000. The lowest laser threshold that we obtained was 0.82 µJ with a microcavity size of 20 µm. The doped fluorescent dye concentration can be tuned precisely from 0.001 to 0.05 wt% to explore an optimized fluorescent background. The experimental results and theoretical simulation match well in terms of Q and the electrometric resonance field intensity. Compared with previous precise and practical fabrication methods, we have demonstrated a facile approach for versatile optical microcavity fabrication. This method can vary the microcavity materials, size, doped fluorescent dye concentration, WGM resonance spectrum, Q factor, and laser threshold easily to adapt to various circumstances and specific applications.

13.
Biosens Bioelectron ; 144: 111663, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31539721

RESUMO

M-nitrophenol (m-NP) is a high priority environmental pollutant and poses a series of threats on human health. Accurate and rapid detection of m-NP in practical samples is very important as this is the key prerequisite for its effective monitoring. Eelectrochemical sensor, though long serving as highly sensitive and fast analytical tool, suffers from the bottleneck problems like low specificity, poor reproducibility, susceptibility to internal and external disturbances, etc. Herein, we developed a ratiometric electrochemical sensor (R-ECS) for m-NP detection, in which nitrogen-doped Mo2C (N-Mo2C) was deployed as the sensing agent and methylene blue (MB) as the internal reference. Full characterization of N-Mo2C was carried out in the aspects of morphology, composition, chemical bonds and electrochemical behavior, and the sensing performance of the easy-to-operate R-ECS was evaluated. Complete separation of the oxidation peaks of m-NP and MB was achieved using the MB/N-Mo2C composite modified electrode and their ratiometric signals were adopted for quantification of m-NP. The linear relation between the electrical signal and the concentration of m-NP is in the range of 1-1500 µM, with the detection limit of 0.256 µM (S/N = 3). The sensor was applied to measure m-NP in real samples from tap water and river. Experimental results demonstrate that it exhibits decent repeatability, reproducibility, stability and selectivity, which proves its great practical potential as an analytical detector.


Assuntos
Técnicas Biossensoriais , Técnicas Eletroquímicas , Nitrofenóis/isolamento & purificação , Água/química , Eletrodos , Humanos , Azul de Metileno/química , Nitrogênio/química , Nitrofenóis/química
14.
Nanotechnology ; 30(49): 495302, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31480026

RESUMO

Sintering of metallic nanoparticles (NPs) at low temperature is highly wanted in the manufacturing of flexible electronics. And for ink-jet printing, the metallic NPs after printing usually need thermal or chemical post-treatment to remove stabilizing agents and achieve conductivity. Here, we reported a facile method to realize one-step printed sintering of silver nanoparticle (AgNP) ink at room temperature by using intermediate coated layers composed of oxide NPs and polyvinyl alcohol (PVA) mixture. We found that the detachment of the stabilizer (citrate) from the AgNPs was caused by hydroxyl groups on the surface of the oxide NPs, which enabled the coalescence and sintering of the AgNPs. With the aid of SiO2 NPs based intermediate layer, the patterns showed resistivity as low as 3.45 µΩ cm after sintering. Moreover, the mixed PVA could ensure the forming quality of patterns owing to its adsorption of ink and the high adhesiveness of PVA with substrates. So, we envision that this approach could serve as an adaptive method for sintering of AgNPs based conductive patterns on various substrates at room temperature and promote the manufacture of printed electronics.

15.
Dalton Trans ; 48(22): 7659-7665, 2019 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-31049511

RESUMO

The development of flexible current collectors as an indispensable component in energy storage devices has been in strong demand for the ever-growing market of flexible and wearable electronics. Herein, flexible and conductive paper-based current collectors are fabricated by directly depositing a metallic Ni layer composed of spiny Ni nanospheres of 400 nm diameter on the surface of filter paper via electroless deposition. The metallic paper shows excellent electric and mechanical properties: the sheet resistance is 2.7 Ω cm-2 (R0 = 0.8 Ω cm-2) after 5000 bending cycles and the mass density is only 0.35 g cm-3. MnO2 is selected as an electrode active material to explore the role of flexible and conductive paper-based current collectors in supercapacitors. Electrochemical results reveal that the largest areal specific capacitance is 1095 mF cm-2 at 1 mA cm-2 and the excellent electrochemical performance can be attributed to the hierarchical porous fibre structure of paper and the lower contact resistance between the active material and the current collector. Note that the approach can be applied to an enlarged size of metallic conductive paper or textile, presenting a simple and feasible method to fabricate flexible current collectors in a large scale.

16.
Int J Mol Med ; 43(4): 1635-1642, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30816515

RESUMO

The present study describes the preparation of a dodecapeptide YHWYGYTPQNVI (GE11)­conjugated liposome bound with polyethylene glycol to enhance the therapeutic effect of resveratrol (RSV) in head and neck cancer cells. The results indicated that (RSV)­loaded GE11­conjugated liposomes (RSV­GL) exhibited a high entrapment efficiency of >95%, with an active drug loading level of 19.5% w/w. Release kinetics revealed that RSV was released in a slow and sustained manner from the RSV­GL and RSV­loaded liposome (RSV­L) nanoparticulate systems. The epidermal growth factor receptor (EGFR)­overexpressing squamous cell carcinoma HN cells specifically internalized GE11 surface­conjugated liposome in a manner that was markedly increased compared with that of the non­targeted carrier. Consistently, RSV­GL exhibited a significantly increased cytotoxic effect compared with that of the non­targeted nanoparticles. Notably, RSV­GL induced significantly increased proportions of early (~60%) and late (~10%) apoptotic cells in head and neck cancer cell populations. To the best of our knowledge, the application and development of EGFR­targeted peptide­conjugated liposome system for RSV delivery has not been studied previously in the treatment of head and neck cancer. In addition, RSV­GL exhibited the greatest antitumor efficacy compared with any other group. RSV­GL exhibited a 2­fold decrease in tumor volume compared with the free RSV and a 3­fold decrease in volume compared with the control. Overall, the nanomedicine strategy described in the present study may potentially advance the chemotherapy­based treatment of head and neck cancer, with promising applications in other EGFR­overexpressing tumors.


Assuntos
Peptídeos/farmacologia , Resveratrol/farmacologia , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Sequência de Aminoácidos , Animais , Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Liberação Controlada de Fármacos , Feminino , Humanos , Lipossomos , Camundongos Nus , Tamanho da Partícula , Peptídeos/química
17.
Electrophoresis ; 40(11): 1580-1590, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30892714

RESUMO

Microfluidics has made a very impressive progress in the past decades due to its unique and instinctive advantages. Droplet-based microfluidic systems show excellent compatibility with many chemical and biological reagents and are capable of performing variety of operations that can implement microreactor, complex multiple core-shell structure, and many applications in biomedical research such as drug encapsulation, targeted drug delivery systems, and multifunctionalization on carriers. Droplet-based systems have been directly used to synthesize particles and encapsulate many biological entities for biomedicine applications due to their powerful encapsulation capability and facile versatility. In this paper, we review its origin, deviation, and evolution to draw a clear future, especially for droplet-based biomedical applications. This paper will focus on droplet generation, variations and complication as starter, and logistically lead to the numerous typical applications in biomedical research. Finally, we will summarize both its challenge and future prospects relevant to its droplet-based biomedical applications.


Assuntos
Pesquisa Biomédica/métodos , Microfluídica/métodos , Encapsulamento de Células , Sistemas de Liberação de Medicamentos , Microfluídica/instrumentação
18.
BMC Infect Dis ; 19(1): 111, 2019 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-30717689

RESUMO

BACKGROUND: Osteoarticular tuberculosis is a great masquerader presenting in varied forms and in atypical locations, and it is prone to misdiagnosis and missed diagnosis. Isolated acromioclavicular joint tuberculosis has been reported rarely. CASE PRESENTATION: A 19-year-old man presented with a chronic, mild pain, non-healing ulcer in right shoulder. Imaging of the shoulder revealed destruction of the acromioclavicular joint and histopathology confirmed the diagnosis of acromioclavicular tuberculosis. The patient underwent debridement, synovectomy and drainage of the abscess and recovered well with antitubercular therapy postoperatively. CONCLUSIONS: Awareness of this uncommon presentation of osteoarticular tuberculosis may assist in earlier diagnosis. Especially, in endemic countries, osteoarticular tuberculosis should be considered as a differential diagnosis in all atypical presentations to avoid residual problems.


Assuntos
Articulação Acromioclavicular/microbiologia , Tuberculose Osteoarticular/diagnóstico , Abscesso/diagnóstico , Articulação Acromioclavicular/patologia , Antituberculosos/uso terapêutico , Desbridamento , Diagnóstico Diferencial , Drenagem , Humanos , Masculino , Dor de Ombro/diagnóstico , Dor de Ombro/tratamento farmacológico , Dor de Ombro/microbiologia , Dor de Ombro/cirurgia , Tuberculose Osteoarticular/tratamento farmacológico , Tuberculose Osteoarticular/cirurgia , Adulto Jovem
19.
Nanotechnology ; 30(18): 185501, 2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-30673645

RESUMO

Patterned circuits on highly stretchable conductive films are critical in the practical application of next-generation flexible and wearable devices. Currently, most patterned circuits do not exhibit highly stretchable properties, and a lithography process in vacuum is required. In this study, silver nanoparticles (AgNPs) and liquid polydimethylsiloxane (PDMS) are mixed together to form liquid conductive adhesives (CAs). Various stretchable patterned circuits are prepared using this CA to achieve all required functions. Six basic patterns, including rhombus, straight lines, serpentine, triangle, ellipses, and fold line, are studied for their stretchable and electrical properties. The film is found to maintain excellent conductivity after withstanding tensile strain of up to 320% and more than 10 000 stretching-releasing cycles of 0%-150%. More than 86% of visible lights can be penetrated through the film due to the transparent substrates. Functional and wearable devices are manufactured, and devices fabricated from rhombus-pattern circuits are found to exhibit stable electrical conductivity when subjected to very high tensile strains. According to the sensitivity of the straight-line patterned circuit to strain, a repeatable use sensitive strain sensor is studied. Also, two types of artificial electrical skin are demonstrated.

20.
Micromachines (Basel) ; 9(2)2018 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30393358

RESUMO

Self-propelling micro/nano-motors have attracted great attention due to their controllable active motion and various functional attributes. To date, a variety of technologies have been reported for the fabrication of micro/nano-motors. However, there are still several challenges that need to be addressed. One of them is to endow micro/nano-motors with multi-functionalities by a facile fabrication process. Here, we present a universal approach, adopted from the emulsion templating method, for the fabrication of Janus micro-motors. With a one-step process, magnetic nanoparticles and fluorescent dyes are simultaneously embedded into the microparticles. The self-propelled motors can be used as an active label or fluorescent tracer through manipulation of their motion using magnetic guidance.

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