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1.
Sci Total Environ ; 866: 161342, 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36603609

RESUMO

Nitrate reduction coupled with arsenic (As) oxidation strongly influences the bioavailability and toxicity of As in anaerobic environments. In the present study, five representative paddy soils developed from different parent materials were used to investigate the universality and characteristics of nitrate reduction coupled with As oxidation in paddy soils. Experimental results indicated that 99.8 % of highly toxic aqueous As(III) was transformed to dissolved As(V) and Fe-bound As(V) in the presence of nitrate within 2-8 d, suggesting that As was apt to be reserved in its low-toxic and nonlabile form after nitrate treatment. Furthermore, nitrate additions also significantly induced the higher abundance of 16S rRNA and As(III) oxidase (aioA) genes in the five paddy soils, especially in the soils developed from purple sand-earth rock and quaternary red clay, which increased by 10 and 3-5 times, respectively, after nitrate was added. Moreover, a variety of putative novel nitrate-dependent As(III)-oxidizing bacteria were identified based on metagenomic analysis, mainly including Aromatoleum, Paenibacillus, Microvirga, Herbaspirillum, Bradyrhizobium, Azospirillum. Overall, all these findings indicate that nitrate reduction coupled with As(III) oxidation is an important nitrogen-As coupling process prevalent in paddy environments and emphasize the significance of developing and popularizing nitrate-based biotechnology to control As pollution in paddy soils and reduce the risk of As compromising food security.

2.
Sci Total Environ ; 854: 158801, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36115399

RESUMO

The environmental behavior of arsenic (As) is commonly affected by the biogeochemical processes of iron (Fe) and nitrogen (N). In this study, field experiments were conducted to explore As uptake in rice and As translation and distribution in As-contaminated iron-rich paddy soils after applying different forms of N fertilizers, including urea (CO(NH2)2), ammonium bicarbonate (NH4HCO3), nitrate of potash (KNO3), and ammonium bicarbonate + nitrate of potash (NH4HCO3 + KNO3). The results indicated that applying nitrate N fertilizer inhibited the reduction and dissolution of As-bearing iron minerals and promoted microbial-mediated As(III) oxidation in flooded soil, thus reducing the soil As bioavailability. The concentrations of total As and inorganic As ratio (iAs/TAs) in rice grain decreased by 32.4 % and 15.4 %, respectively. However, the application of ammonium nitrogen promoted the reductive dissolution of As-bearing iron minerals and stimulated microbial As(V) reduction in flooded soil, leading to the release of As from soil to porewater. The total As concentration and inorganic As uptake ratio in rice grain increased by 20.1 % and 6.2 %, respectively, when urea was applied, and by 29.6 % and 10.5 %, respectively, when ammonium bicarbonate was applied. However, the simultaneous application of NH4+ and NO3- had no significant effect on As concentration in rice grain and its transformation in paddy soils. Ammonium nitrogen enhanced the organic As concentration in rice grain because the increased As(III) promoted As methylation in soil. In contrast, nitrate decreased the organic As uptake by rice grain because the decreased As(III) diminished As methylation in soil. The results provide reasonable N fertilization strategies for regulating the As biogeochemical process and reducing the risk of As contamination in rice.

3.
J Healthc Eng ; 2022: 2004973, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35432838

RESUMO

Objective: To study the effect of apatinib combined with seggio on the expression of serum AFP and CA724 and the long-term survival rate in advanced gastric cancer patients undergoing comfort nursing intervention. Methods: 98 advanced gastric cancer patients were divided into single-drug group and joint group. Both groups of patients were given comfort nursing intervention, the single-drug group was treated with seggio, and the joint group was treated with apatinib and seggio. The clinical efficacy, survival rate, relationship between the tumor markers and the survival time, serum tumor markers levels (CA724 and AFP), inflammatory factors (IL-4, IL-10) levels, quality-of-life scores, and immunity function were measured after treatment. Results: The clinical efficacy in the joint group was better than that in the single-drug group. The three-year survival time in the joint group was upregulated relative to the single-drug group. The patients with high expression of CA724 or AFP had a lower survival time than the patients with low expression of CA724 or AFP. After treatment, IL-10 and IL-4 levels were obviously decreased, and the joint group showed a more obvious decrease compared with the single-drug group. The quality-of-life scores were significantly upregulated after treatment, and compared with the joint group, the scores in the single drug-group were obviously higher. The CD4+/CD8+, CD4+, and CD3+ levels were increased, while CD8+ levels were decreased after treatment, and the changes of each index in the joint group were more significant than those in the single-drug group. The content of CA724 and AFP were significantly decreased after treatment, and the joint group showed a more significant decrease than the single-drug group. Conclusion: Apatinib combined with seggio for advanced gastric cancer patients' treatment based on comfort nursing intervention can improve the clinical efficacy and survival time, reduce inflammatory factors and serum tumor markers levels, enhance patients' immune function, and quality of life.


Assuntos
Interleucina-10 , Neoplasias Gástricas , Biomarcadores Tumorais/uso terapêutico , Humanos , Interleucina-10/uso terapêutico , Interleucina-4/uso terapêutico , Piridinas , Qualidade de Vida , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/patologia , Taxa de Sobrevida , alfa-Fetoproteínas/uso terapêutico
4.
IEEE Trans Vis Comput Graph ; 28(3): 1500-1513, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32833636

RESUMO

During exploratory visual analysis (EVA), analysts need to continually determine which subsequent activities to perform, such as which data variables to explore or how to present data variables visually. Due to the vast combinations of data variables and visual encodings that are possible, it is often challenging to make such decisions. Further, while performing local explorations, analysts often fail to attend to the holistic picture that is emerging from their analysis, leading them to improperly steer their EVA. These issues become even more impactful in the real world analysis scenarios where EVA occurs in multiple asynchronous sessions that could be completed by one or more analysts. To address these challenges, this work proposes ChartSeer, a system that uses machine intelligence to enable analysts to visually monitor the current state of an EVA and effectively identify future activities to perform. ChartSeer utilizes deep learning techniques to characterize analyst-created data charts to generate visual summaries and recommend appropriate charts for further exploration based on user interactions. A case study was first conducted to demonstrate the usage of ChartSeer in practice, followed by a controlled study to compare ChartSeer's performance with a baseline during EVA tasks. The results demonstrated that ChartSeer enables analysts to adequately understand current EVA status and advance their analysis by creating charts with increased coverage and visual encoding diversity.

5.
J Hazard Mater ; 424(Pt B): 127545, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34879531

RESUMO

In this study, Aspergillus sydowii FJH-1 isolated from soil was verified to be a novel triphenyl phosphate (TPhP) degrader. Biodegradation efficiency of TPhP by Aspergillus sydowii FJH-1 exceeded 90% within 6 days under the optimal conditions (pH 4-9, 30 â„ƒ, initial concentration less than 20 mg/L). Proteomics analysis uncovered the proteins perhaps involved in hydrolysis, hydroxylation, methylation and sulfonation of TPhP and the primary intracellular adaptive responses of Aspergillus sydowii FJH-1 to TPhP stress. The expression of carboxylic ester hydrolase along with several thioredoxin- and glutathione-dependent oxidoreductases were induced to withstand the toxicity of TPhP. The presence of TPhP also caused obvious upregulation of proteins concerned with glycolysis, pentose phosphate pathway and tricarboxylic acid cycle. Data from toxicological tests confirmed that the cytotoxicity and phytotoxicity of TPhP was effectively decreased after treatment with Aspergillus sydowii FJH-1. Additionally, bioaugmentation with Aspergillus sydowii FJH-1 was available for promoting TPhP removal in real water and water-sediment system. Collectively, the present study offered a deeper insight into the biodegradation mechanism and pathway of TPhP by a newly screened fungal strain Aspergillus sydowii FJH-1 and validated the feasibility of applying this novel degrader in the bioremediation of TPhP-polluted matrices.


Assuntos
Organofosfatos , Rios , Biodegradação Ambiental , Proteômica
6.
Int J Biol Macromol ; 193(Pt A): 347-357, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34695496

RESUMO

It is important to convert waste shrimp shells into chitin and its derivatives, but it still faces disadvantages of pollution and long process. In this research, deep eutectic solvents made from choline chloride (ChCl) and p-toluenesulfonic acid monohydrate (TsOH) were used to convert shrimp shells into chitin or carbon materials through adjustment of temperature with a dissolution-regeneration method in one step. Obtained precipitates and regenerated products gradually changed from chitin to carbon materials with the temperature adjusted to 130 °C and 110 °C, respectively. The α-chitin with the highest purity of 97.9 ± 0.1 wt% was prepared using 15 wt% ChCl/TsOH (1:2, n:n) aqueous solution at 70 °C, and the yield was 59.4 ± 1.9 wt%. Moreover, the carbon materials prepared by ChCl/TsOH (1:4) treatment with water and ethanol as anti-solvent were S/N/O-doped carbon materials, which exhibited potential in the supercapacitor with a specific capacitance of 24.8 and 19.3 F/g at 1 A/g, respectively.


Assuntos
Quitina/química , Colina/química , Crustáceos/metabolismo , Solventes Eutéticos Profundos/química , Animais
7.
J Hazard Mater ; 417: 125983, 2021 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-33975170

RESUMO

So far, no information about the biodegradability of TPhP by white rot fungi has previously been made available, herein, Pycnoporus sanguineus was used as the representative to investigate the potential of white rot fungi in TPhP bioremediation. The results suggested that the biodegradation efficiency of 5 mg/L TPhP by P. sanguineus was 62.84% when pH was adjusted to 6 and initial glucose concentration was 5 g/L. Seven biodegradation products were identified, indicating that TPhP was biotransformed through oxidative cleavage, hydroxylation and methylation. The proteomic analysis revealed that cytochrome P450s, aromatic compound dioxygenase, oxidizing species-generating enzymes, methyltransferases and MFS general substrate transporters might occupy important roles in TPhP biotransformation. Carboxylesterase and glutathione S-transferase were induced to resist TPhP stress. The biotreatment by P. sanguineus contributed to a remarkable decrease of TPhP biotoxicity. Bioaugmentation with P. sanguineus could efficiently promote TPhP biodegradation in the water-sediment system due to the cooperation between P. sanguineus and some putative indigenous degraders, including Sphingobium, Burkholderia, Mycobacterium and Methylobacterium. Overall, this study provided the first insights into the degradation pathway, mechanism and security risk assessment of TPhP biodegradation by P. sanguineus and verified the feasibility of utilizing this fungus for TPhP bioremediation applications.


Assuntos
Organofosfatos , Proteômica , Biodegradação Ambiental , Redes e Vias Metabólicas , Polyporaceae
9.
Carbohydr Polym ; 256: 117580, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-33483074

RESUMO

Hydrogels are widely used in many fields but generally suffer from low mechanical strength and poor self-healing performance. Here, a novel and facile method was developed to prepare a semi-interpenetrating polymer network (semi-IPN) hydrogel with layered structure and improved properties based on sodium alginate (SA) and polyacrylamide (PAM). Systematic characterizations revealed a formation mechanism of layered structure via hydrogen bonds (HBs) promoted self-assembly of SA in the porous PAM matrix. Also, HBs can also display a key role in enhancing self-healing of the hydrogel, by which the hydrogel possesses a self-healing capacity of 99 % with sprayed by a few of water. Moreover, the layered semi-IPN structure makes the tensile strength of PAMSA hydrogel reach 266 kPa. The fabricated PAMSA hydrogel with layered microstructure containing SA provides a protocol to broaden the functionality and variety of the hydrogels.

10.
J Environ Sci (China) ; 100: 90-98, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33279057

RESUMO

Nitrate (NO3-) is known to be actively involved in the processes of mineralization and heavy metal transformation; however, it is unclear whether and how it affects the bioavailability of antimony (Sb) in paddy soils and subsequent Sb accumulation in rice. Here, the effects of NO3- on Sb transformation in soil-rice system were investigated with pot experiments over the entire growth period. Results demonstrated that NO3- reduced Sb accumulation in brown rice by 15.6% compared to that in the control. After amendment with NO3-, the Sb content in rice plants increased initially and then gradually decreased (in roots by 46.1%). During the first 15 days, the soil pH increased, the oxidation of Sb(III) and sulfides was promoted, but the reduction of iron oxide minerals was inhibited, resulting in the release of adsorbed and organic-bound Sb from soil. The microbial arsenite-oxidizing marker gene aoxB played an important role in Sb(III) oxidation. From days 15 to 45, after NO3- was partially consumed, the soil pH decreased, and the reductive dissolution of Fe(III)-bearing minerals was enhanced; consequently, iron oxide-bound Sb was transformed into adsorbed and dissolved Sb species. After day 45, NO3- was completely reduced, Sb(V) was evidently reduced to Sb(III), and green rust was generated gradually. Thus, the available Sb decreased due to its enhanced affinity for iron oxides. Moreover, NO3- inhibited the reductive dissolution of iron minerals, which ultimately caused low Sb availability. Therefore, NO3- can chemically and biologically reduce the Sb availability in paddy soils and alleviate Sb accumulation in rice. This study provides a potential strategy for decreasing Sb accumulation in rice in the Sb-contaminated sites.


Assuntos
Oryza , Poluentes do Solo , Antimônio/análise , Disponibilidade Biológica , Compostos Férricos , Nitratos , Raízes de Plantas/química , Solo , Poluentes do Solo/análise
11.
IEEE Trans Vis Comput Graph ; 27(2): 1063-1072, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33296303

RESUMO

Data visualization is powerful in large part because it facilitates visual extraction of values. Yet, existing measures of perceptual precision for data channels (e.g., position, length, orientation, etc.) are based largely on verbal reports of ratio judgments between two values (e.g., [7]). Verbal report conflates multiple sources of error beyond actual visual precision, introducing a ratio computation between these values and a requirement to translate that ratio to a verbal number. Here we observe raw measures of precision by eliminating both ratio computations and verbal reports; we simply ask participants to reproduce marks (a single bar or dot) to match a previously seen one. We manipulated whether the mark was initially presented (and later drawn) alone, paired with a reference (e.g. a second '100%' bar also present at test, or a y-axis for the dot), or integrated with the reference (merging that reference bar into a stacked bar graph, or placing the dot directly on the axis). Reproductions of smaller values were overestimated, and larger values were underestimated, suggesting systematic memory biases. Average reproduction error was around 10% of the actual value, regardless of whether the reproduction was done on a common baseline with the original. In the reference and (especially) the integrated conditions, responses were repulsed from an implicit midpoint of the reference mark, such that values above 50% were overestimated, and values below 50% were underestimated. This reproduction paradigm may serve within a new suite of more fundamental measures of the precision of graphical perception.

12.
Bioorg Chem ; 116: 105303, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34464815

RESUMO

Eucalyptus is a large genus of the Myrtaceae family with high value in various fields of industry. Recently, attention has been focused on the functional properties of Eucalyptus extracts. These extracts have been traditionally used to combat various infectious diseases, and volatile oils are usually considered to play a major role. But the positive effects of non-volatile acylphloroglucinols, a class of specialized metabolites with relatively high content in Eucalyptus, should not be neglected. Herein, non-volatile acylphloroglucinols from leaves of Eucalyptus robusta were evaluated for their abilities to inhibit Zika virus (ZIKV) which is associated with severe neurological damage and complications. The results showed eucalyprobusone G, a new symmetrical acylphloroglucinol dimer, possessed the significant ability to inhibit ZIKV without inducing cytotoxicity. The EC50 values of eucalyprobusone G against the African lineage (MR766) and Asian lineage (SZ-WIV01) of ZIKV were 0.43 ± 0.08 and 10.10 ± 3.84 µM which were 110 times and 5.8 times better than those of the reference compound ribavirin, respectively. Further action mode research showed that eucalyprobusone G impairs the viral binding and RdRp activity of NS5. The results broaden the functional properties of Eucalyptus robusta and indicate acylphloroglucinol dimers could be developed as anti-ZIKV agents.


Assuntos
Antivirais/farmacologia , Eucalyptus/química , Floroglucinol/farmacologia , Zika virus/efeitos dos fármacos , Animais , Antivirais/química , Antivirais/isolamento & purificação , Linhagem Celular , Chlorocebus aethiops , Relação Dose-Resposta a Droga , Humanos , Testes de Sensibilidade Microbiana , Estrutura Molecular , Floroglucinol/química , Floroglucinol/isolamento & purificação , Folhas de Planta/química , Relação Estrutura-Atividade
13.
IEEE Trans Vis Comput Graph ; 27(2): 475-484, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33048720

RESUMO

We present an integrated approach for creating and assigning color palettes to different visualizations such as multi-class scatterplots, line, and bar charts. While other methods separate the creation of colors from their assignment, our approach takes data characteristics into account to produce color palettes, which are then assigned in a way that fosters better visual discrimination of classes. To do so, we use a customized optimization based on simulated annealing to maximize the combination of three carefully designed color scoring functions: point distinctness, name difference, and color discrimination. We compare our approach to state-of-the-art palettes with a controlled user study for scatterplots and line charts, furthermore we performed a case study. Our results show that Palettailor, as a fully-automated approach, generates color palettes with a higher discrimination quality than existing approaches. The efficiency of our optimization allows us also to incorporate user modifications into the color selection process.

14.
Bioorg Chem ; 103: 104127, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32745755

RESUMO

Eleven new acylphloroglucinols, including six new formylated phloroglucinol-monoterpene meroterpenoids, eucalyprobusals A-F (1-6), one monomeric acylphloroglucinol, eucalyprobusone B (7), and four dimeric acylphloroglucinols, eucalyprobusones C-F (8-11) were purified from the fruits of Eucalyptus robusta. The establishment of the structures of 1-11 was achieved by a combination of NMR and HRESIMS data analyses, electron circular dichroism (ECD), and single-crystal X-ray diffraction. Compounds 6, 8, and an inseparable mixture of 10 and 11 were found to be potent AChE inhibitors with IC50 values of 3.22 ± 0.36, 3.82 ± 0.22, and 2.55 ± 0.28 µΜ, respectively. Possible interaction sites of 6, 8, 10, and 11 with AChE were investigated by means of molecular docking studies, and the results revealed that AChE residues Asn87, Ser125, Thr83, Tyr133, Tyr124, Tyr337, and Tyr341 played crucial roles in the observed activity of the aforementioned compounds.


Assuntos
Acetilcolinesterase/metabolismo , Inibidores da Colinesterase/farmacologia , Eucalyptus/química , Frutas/química , Floroglucinol/farmacologia , Inibidores da Colinesterase/química , Inibidores da Colinesterase/isolamento & purificação , Teoria da Densidade Funcional , Relação Dose-Resposta a Droga , Humanos , Simulação de Acoplamento Molecular , Estrutura Molecular , Floroglucinol/química , Floroglucinol/isolamento & purificação , Relação Estrutura-Atividade
15.
Nat Commun ; 10(1): 4677, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31597915

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

16.
Chemosphere ; 235: 995-1006, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31561316

RESUMO

The influence of Cr(VI) on the degradation of tetrabromobisphenol A (TBBPA) by a typical species of white rot fungi, Pycnoporus sanguineus, was investigated in this study. The results showed that P. sanguineus together with its intracellular and extracellular enzyme could effectively degrade TBBPA. The degradation efficiency of TBBPA by both P. sanguineus and its enzymes decreased significantly when Cr(VI) concentration increased from 0 to 40 mg/L. The subsequent analysis about cellular distribution of TBBPA showed that the extracellular amount of TBBPA increased with the increment of Cr(VI) concentration, but the content of TBBPA inside fungal cells exhibited an opposite variation tendency. The inhibition of TBBPA degradation by P. sanguineus was partly attributed to the increase of cell membrane permeability and the decrease of cell membrane fluidity caused by Cr(VI). In addition, the decline of H+-ATPase and Mg2+-ATPase activities was also an important factor contributing to the suppression of TBBPA degradation in the system containing concomitant Cr(VI). Moreover, the activities of two typical extracellular lignin-degrading enzymes of P. sanguineus, MnP and Lac, were found to descend with ascended Cr(VI) level. Cr(VI) could also obviously suppress the gene expression of four intracellular enzymes implicated in TBBPA degradation, including two cytochrome P450s, glutathione S-transferases and pentachlorophenol 4-monooxygenase, which resulted in a decline of TBBPA degradation efficiency by fungal cells and intracellular enzyme in the presence of Cr(VI). Overall, this study provides new insights into the characteristics and mechanisms involved in TBBPA biodegradation by white rot fungi in an environment where heavy metals co-exist.


Assuntos
Biodegradação Ambiental , Cromo/toxicidade , Poluentes Ambientais/metabolismo , Bifenil Polibromatos/metabolismo , Pycnoporus/metabolismo , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Proteínas Fúngicas/metabolismo , Oxirredução , Pycnoporus/efeitos dos fármacos , Pycnoporus/crescimento & desenvolvimento
17.
Nat Commun ; 10(1): 3748, 2019 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-31444336

RESUMO

Epidemic spreading processes in the real world depend on human behaviors and, consequently, are typically non-Markovian in that the key events underlying the spreading dynamics cannot be described as a Poisson random process and the corresponding event time is not exponentially distributed. In contrast to Markovian type of spreading dynamics for which mathematical theories have been well developed, we lack a comprehensive framework to analyze and fully understand non-Markovian spreading processes. Here we develop a mean-field theory to address this challenge, and demonstrate that the theory enables accurate prediction of both the transient phase and the steady states of non-Markovian susceptible-infected-susceptible spreading dynamics on synthetic and empirical networks. We further find that the existence of equivalence between non-Markovian and Markovian spreading depends on a specific edge activation mechanism. In particular, when temporal correlations are absent on active edges, the equivalence can be expected; otherwise, an exact equivalence no longer holds.

18.
ChemSusChem ; 12(17): 4005-4013, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31291505

RESUMO

The degradation of lignin into aromatic products is very important, but harsh conditions and metal-based catalysts are commonly needed to cleave the inert bonds. Herein, an efficient self-initiated radical photochemical degradation for lignin-derived aryl ethers through ionic liquids (ILs) induction is demonstrated. The C-C/C-O bonds can be cleaved efficiently through free-radical-mediated reaction in the binary-ILs system 1-propenyl-3-methylimidazolium bis[(trifluoromethyl)sulfonyl] imide [PMim][NTf2 ] and the Brønsted acid 1-propylsulfonic-3-methylimidazolium trifluoromethanesulfonate ([PrSO3 HMim][OTf]) under ambient conditions. [PMim][NTf2 ] initiates the reaction by promoting the cleavage of the Cß -H bond, and [PrSO3 HMim][OTf] catalyzes the subsequent C-O-C bond fragmentation. Furthermore, alkyl, hydroxyl, and peroxy radicals are detected, which suggests degradation based on a photochemical free-radical process. Additionally, alkali lignin could also be degraded in the IL system. This work sheds light on sustainable biomass utilization through a self-initiated radical photochemical strategy under metal-free and mild conditions.

19.
J Environ Sci (China) ; 83: 133-143, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31221376

RESUMO

The degradation of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) by Pycnoporus sanguineus was investigated in order to explore the impact of the heavy metal Cu2+ on BDE-47 decomposition and the subsequent formation of metabolites, as well as to further elucidate the degradation mechanism of BDE-47. An increase in degradation rate from 18.63% to 49.76% in the first four days and its stabilization at (51.26 ±â€¯0.08)% in the following days of BDE-47 incubation were observed. The presence of Cu2+ at 1 and 2 mg/L was found to promote the degradation rate to 56.41% and 60.79%, respectively, whereas higher level of Cu2+ (≥5 mg/L) inhibited the removal of BDE-47. The similar concentration effects of Cu2+ was also found on contents of fungal protein and amounts of metabolites. Both intracellular and extracellular enzymes played certain roles in BDE-47 transportation with the best degradation rate at 27.90% and 27.67% on the fourth and third day, individually. During the degradation of BDE-47, four types of hydroxylated polybrominated diphenyl ethers (OH-PBDEs), i.e., 6'-OH-BDE-47, 5'-OH-BDE-47, 4'-OH-BDE-17, 2'-OH-BDE-28, and two bromophenols, i.e., 2,4-DBP and 4-BP were detected and considered as degradation products. These metabolites were further removed by P. sanguineus at rates of 22.42%, 23.01%, 27.04%, 27.96%, 64.21%, and 40.62%, respectively.


Assuntos
Biodegradação Ambiental , Cobre/metabolismo , Poluentes Ambientais/metabolismo , Éteres Difenil Halogenados/metabolismo , Pycnoporus/metabolismo
20.
J Cell Biochem ; 120(9): 16244-16253, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31081255

RESUMO

BACKGROUND: Wound healing is a complex process in bone development. The aim of this study was to explore the molecular mechanism study of insulin in promoting wound healing. METHODS: Firstly, the acute human monocyte leukemia cell lines were induced to differentiate into macrophages. Secondly, the porphyromonas gingivalis was applied to mix with the differentiated macrophages. Thirdly, the effect of different concentrations of insulin (0 ng/mL, 5 ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL, 500 ng/mL, and 1,000 ng/mL) on the phagocytosis of macrophages and production of reactive oxygen species was investigated. Depending on these experiments, the optimal insulin concentration was used to treat the macrophages at different time points (0 hours and 0.5 hours) to identify the differentially expressed mRNAs. Finally, functional analysis including gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) analysis was carried out to explore the biological function of these differentially expressed mRNAs. RESULTS: The test of phagocytosis function and production of reactive oxygen species showed that 200 ng/mL insulin treatment had a significant influence on antibacterial and production of reactive oxygen species. In RNA sequencing, a total of 415 (245 upregulated and 170 downregulated) differentially expressed mRNAs were identified between different time points. Two important signaling pathways including endocytosis and systemic lupus erythematosus were found in the KEGG enrichment analysis. In the PPI network, several hub proteins encoded by differentially expressed mRNA including ALB, HIP1R, RAB5A, HIST1H2BJ, HIST1H3G, and HIST1H2BO were identified. CONCLUSION: Our work demonstrated that several differentially expressed mRNAs, such as EGR1, RAB34, ALB, HIP1R, RAB5A, HIST1H2BJ, HIST1H3G, and HIST1H2BO and two important signaling pathways including endocytosis and systemic lupus erythematosus may play important roles in the bone wound healing.


Assuntos
Perfilação da Expressão Gênica/métodos , Redes Reguladoras de Genes , Glucose/farmacologia , Insulina/farmacologia , Macrófagos/citologia , Diferenciação Celular , Linhagem Celular Tumoral , Ontologia Genética , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Porphyromonas gingivalis/efeitos dos fármacos , Mapas de Interação de Proteínas , Espécies Reativas de Oxigênio/metabolismo , Análise de Sequência de RNA , Cicatrização
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